CN205974498U - Visual leakproofness nucleic acid amplifys and test paper detection device - Google Patents
Visual leakproofness nucleic acid amplifys and test paper detection device Download PDFInfo
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- CN205974498U CN205974498U CN201620979560.0U CN201620979560U CN205974498U CN 205974498 U CN205974498 U CN 205974498U CN 201620979560 U CN201620979560 U CN 201620979560U CN 205974498 U CN205974498 U CN 205974498U
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Abstract
The utility model discloses a visual leakproofness nucleic acid amplifys and test paper detection device, including upper cover (1), nucleic acid test test paper strip (2) and the pipe (3) that amplifys, top center department in the upper cover is equipped with clamping part (11), the upper end and the clamping part joint of nucleic acid test test paper strip, the lower extreme of nucleic acid test test paper strip is equipped with the portion of absorbing water (21) that is used for absorbing nucleic acid increase reagent, be equipped with snap ring (12) of evagination on the lateral wall of upper cover, be equipped with draw -in groove (31) that match with the snap ring on the inside wall of increase pipe upper end, center department of increase socle portion is equipped with recessed nucleic acid increase reagent storage unit (32). The utility model discloses need not uncap after simple structure, easy, with low costs, the easy operation of preparation, nucleic acid amplify with constant temperature and direct test paper strip detects, be suitable for the witnessed inspections and grassroots staff uses.
Description
Technical field
This utility model is related to the fast qualitative detection technical field of gene isothermal amplification, close particularly to a kind of visualization
Envelope property nucleic acid amplification and detection paper device.
Background technology
With scientific and technical progress, detection of nucleic acids is because the spy such as specificity is good, sensitivity is high, detection speed is relatively fast
Put and be widely used in the fields such as medical diagnosiss, food safety detection.Due to conventional detection of nucleic acids need to use more
Expensive, precision instrument（As nucleic acid augmentative instrument, electrophresis apparatuses etc.）, detection time is relatively long, be unfavorable for device miniaturization exploitation
Be applied to Site Detection.Therefore, in recent years, some new isothermal amplification technologies have developed rapidly, such as cross primer constant-temperature amplification
Technology（CPA）, loop-mediated isothermal amplification technology（LAMP）, strand displacement amplification（SDA）, rely on unwindase amplification（HDA）Deng.This
A little constant temperature nucleic acid amplification technology have started to be applied to field of fast detection, in combination with emerging non-optical detection method such as electrification
Method, colorimetry, nephelometry, and immune colloid gold nucleic acid test strip is it is achieved that quick detection to target nucleic acid, these
The requirement to device of constant-temperature amplification and detection technique is greatly simplified it is only necessary to water bath with thermostatic control or metal bath can be achieved with nucleic acid expansion
Increase, the operation but needs that have of these methods are uncapped, be easy to cause Aerosol Pollution, some detection sensitivities are not high, result is not
Easily judge.Therefore, develop airtight, miniaturization, portable nucleic acid detection apparatus, it is combined with existing detection method aobvious
Obtain more and more important.
The nucleic acid Rapid detection test strip of gold colloidal formula is because of its detection specificity, high sensitivity and simply portable characteristic
Receive significant attention, field quick detection generally will coordinate disposable detection means to use when using.But it is business-like at present
The design of Rapid detection test strip device is relative complex, and production cost is of a relatively high, is unfavorable for popularization and application.Therefore develop easy to be easy
OK, nucleic acid amplification with low cost and ELISA test strip integrated apparatus, very necessary.
Content of the invention
The purpose of this utility model is to provide that a kind of structure is simple, make easily, low cost, simple to operate, nucleic acid permanent
Do not need to uncap after temperature amplification and direct ELISA test strip, be suitable for the visualization sealing that Site Detection and basic staff use
Nucleic acid amplification and detection paper device.
This utility model solves its technical problem and be employed technical scheme comprise that：
A kind of visualization sealing nucleic acid amplification and detection paper device, including on cover（1）, nucleic acid detection test strip（2）
And amplification pipe（3）, at the top center in described upper lid, it is provided with clamping part（11）, the upper end of described nucleic acid detection test strip and folder
Hold portion's clamping, the lower end of described nucleic acid detection test strip is provided with the water-holding portion for absorbing nucleic acid amplification agents（21）, described on
The lateral wall of lid is provided with the snap ring of evagination（12）, the medial wall of described amplification pipe upper end is provided with the draw-in groove matching with snap ring
（31）, described amplification bottom of the tube center is provided with recessed nucleic acid amplification agents storage part（32）.
Preferably, described upper lid includes top cover（13）And lower extending part（14）, it is straight that the upper diameter of lower extending part is more than bottom
Footpath, lower extending part is in integrally inverted convex.
Preferably, described snap ring includes snap ring and lower snap ring, upper snap ring is located at the top of lower extending part, and lower snap ring is located at
The bottom of lower extending part.
Preferably, described amplification pipe includes pipe portion（33）And down leg（34）, upper pipe portion with diameter greater than down leg,
Described nucleic acid amplification agents storage part is connected with the bottom of down leg.
Preferably, described draw-in groove includes draw-in groove, middle draw-in groove and lower bayonet slot, upper draw-in groove is located at the upper end of upper pipe portion, in
Draw-in groove is located at the lower end of upper pipe portion, and lower bayonet slot is located at the upper end of down leg.
Preferably, described clamping part is made up of two panels Plastic clamping piece, the gap of two panels Plastic clamping piece is 1mm.Because big
The sheet thickness about 1-1.5mm of partial immune colloid gold nucleic acid detection test strip, such immune colloid gold detection of nucleic acids reagent paper
Bar can directly be clamp-oned two panels Plastic clamping piece and fix test strips.
Preferably, a diameter of 6-7mm of described nucleic acid amplification agents storage part, depth is 5mm.Because usual nucleic acid expands
Liquid required for increasing is considerably less, and in 10-100uL, therefore, nucleic acid amplification agents storage part portion is arranged to will than amplification pipe diameter
Little reactive site.The reagent of nucleic acid amplification, such as enzyme, probe, dNTP etc. all deposits in nucleic acid amplification agents with vitrification form
Storage part, amount is generally in 5-20uL.
Preferably, when upper snap ring is engaged with upper draw-in groove, the bottom of nucleic acid detection test strip and nucleic acid amplification agents are deposited
The top putting portion flushes.At this point it is possible to put into this utility model carry out constant-temperature amplification in water bath with thermostatic control or constant-temperature metal bath.On
Snap ring acts primarily as spacing and sealing function with upper draw-in groove when engaging, now, the water-holding portion of nucleic acid detection test strip lower end is just not
Enter in nucleic acid amplification agents storage part it is ensured that in constant-temperature amplification, nucleic acid amplification agents will not be drawn onto.
Preferably, engage with middle draw-in groove when upper snap ring, when lower snap ring is engaged with lower bayonet slot, the suction of nucleic acid detection test strip
Water portion is located in nucleic acid amplification agents storage part.When being detected after the completion of constant-temperature amplification step, firmly cover in pressing again, when
When upper snap ring is engaged with middle draw-in groove, lower snap ring is engaged with lower bayonet slot, now, the water-holding portion of nucleic acid detection test strip puts entrance nucleic acid
Amplifing reagent storage part, water-holding portion is totally submerged in nucleic acid amplification agents.
Preferably, described amplification pipe is made up of transparent polystyrene plastics or polyester plasticses.Amplification pipe adopts full impregnated
Bright, higher temperature resistant polystyrene or polyester material plastics it is ensured that during nucleic acid test strip detection colour developing detection line and nature controlling line from
High-visible, the constant temperature in conventional nucleic acid constant-temperature amplification technology simultaneously in outside（Generally 60-70oC）Lower stable, do not interfere with
Amplification and transparency etc..
The beneficial effects of the utility model are：Structure is simple, make easy, low cost, simple to operate, amplification process
In hermetically sealed, the detection after amplification does not need to uncap only needs directly to press lid operation, does not have Aerosol, inspection
Test agent does not leak, and can focus on, and does not result in the pollution of environment.
Brief description
Fig. 1 is a kind of side view of upper lid of the present utility model.
Fig. 2 is a kind of top view of upper lid of the present utility model.
Fig. 3 is a kind of side view of amplification pipe of the present utility model.
Fig. 4 be of the present utility model assembling after expand when a kind of topology view.
Fig. 5 be of the present utility model assembling after detect when a kind of topology view.
In figure：1st, Shang Gai, 11, clamping part, 12, snap ring, 13, top cover, 14, lower extending part, 2, nucleic acid detection test strip, 21,
Water-holding portion, 3, amplification pipe, 31, draw-in groove, 32, nucleic acid amplification agents storage part, 33, upper pipe portion, 34, down leg.
Specific embodiment
Below by specific embodiment, and combine accompanying drawing, the technical solution of the utility model is made further specifically
Bright.
Embodiment：
A kind of visualization sealing nucleic acid amplification and detection paper device, including on lid 1, nucleic acid detection test strip 2 and expand
Increase pipe 3, described amplification pipe is made up of transparent polystyrene plastics or polyester plasticses.Described upper lid includes top cover 13 and lower extending part
14, the upper diameter of lower extending part is more than lower diameter, and lower extending part is in integrally inverted convex；At top cap central in described upper lid
It is provided with clamping part 11, clamping part is made up of two panels Plastic clamping piece, the gap of two panels Plastic clamping piece is 1mm, nucleic acid detection test strip
Upper end directly clamp-on the gap of two panels Plastic clamping piece, thus with clamping part clamping.The lower end of described nucleic acid detection test strip sets
There is the water-holding portion 21 for absorbing nucleic acid amplification agents, the lateral wall of described upper lid is provided with the snap ring 12 of evagination, described amplification
The medial wall of pipe upper end is provided with the draw-in groove 31 matching with snap ring.Described amplification pipe includes pipe portion 33 and down leg 34, upper pipe
Portion with diameter greater than down leg, be provided with recessed nucleic acid amplification agents storage part 32 at down leg bottom centre, described nucleic acid expands
Increase a diameter of 6-7mm in reagent storage portion, depth is 5mm, described nucleic acid amplification agents storage part is connected with the bottom of down leg.
Described snap ring includes snap ring and lower snap ring, and upper snap ring is located at the top of lower extending part, and lower snap ring is located at lower extending part
Bottom.Described draw-in groove includes draw-in groove, middle draw-in groove and lower bayonet slot, and upper draw-in groove is located at the upper end of upper pipe portion, and middle draw-in groove is located at upper pipe
The lower end in portion, lower bayonet slot is located at the upper end of down leg.When upper snap ring is engaged with upper draw-in groove, the bottom of nucleic acid detection test strip with
The top of nucleic acid amplification agents storage part flushes.Engage with middle draw-in groove when upper snap ring, when lower snap ring is engaged with lower bayonet slot, nucleic acid is examined
The absorbent section of test paper slip is in nucleic acid amplification agents storage part.
The use of this utility model patent taking cow genome detection of nucleic acids is as a example described.
Nucleic acid amplification agents storage part 32 is used for the polymerase of cow genome detection of nucleic acids, probe, primer, dNTP containing 5uL
Deng CPA（Cross primer constant-temperature amplification）Reagent, and existed with vitrification form.Add 15uL toward nucleic acid amplification agents storage part 32
Redissolve buffer, then Deca 20uL paraffin oil, room temperature stands 2-3 minute makes glassing reagent fully dissolve.By cow genome nucleic acid
Test strip upper end is forcibly inserted in the gap of clamping part 11 two panels Plastic clamping piece, standby.Deposit toward nucleic acid amplification agents
Portion 32 adds the nucleic acid extraction liquid of 5uL sample to be determined, after being mixed with pipettor piping and druming, will be with cow genome detection of nucleic acids reagent paper
The upper lid 1 of bar is inserted into amplification pipe, firmly slightly covers in pressure, makes the upper snap ring of lid enter into the upper draw-in groove engaging of amplification pipe, this
When amplification pipe be in air-tight state.Now, amplification pipe is placed in constant-temperature amplification 60min in 60 DEG C of water-baths.After amplification terminates, take out
Device, firmly cover in pressing again, so that lid is moved down, and the upper snap ring of upper lid is engaged with the middle draw-in groove expanding pipe, upper lid
Lower snap ring is engaged with the lower bayonet slot of amplification pipe, and now, the water-holding portion 21 of cow genome nucleic acid detection test strip enters into nucleic acid amplification
Reagent storage portion 32, and be immersed in amplification liquid, specific nucleic acid fragment up can be moved together with liquid, when running into inspection
In test paper slip during the specific antibody of embedding, it is combined with each other, presents a red line, display is positive.If do not had in sample
Bovine nucleic acid fragment, then do not have amplified reaction, finally would not be combined with the specific antibody of embedding in test strip,
Also will not show red, display is negative.
Embodiment described above is one kind of the present utility model preferably scheme, not this utility model is made any
Pro forma restriction, also has other variants and remodeling on the premise of without departing from the technical scheme described in claim.
Claims (10)
1. a kind of visualization sealing nucleic acid amplification and detection paper device it is characterised in that：Including on cover（1）, detection of nucleic acids
Test strips（2）And amplification pipe（3）, at the top center in described upper lid, it is provided with clamping part（11）, described nucleic acid detection test strip
Upper end and clamping part clamping, the lower end of described nucleic acid detection test strip is provided with the water-holding portion for absorbing nucleic acid amplification agents
（21）, the lateral wall of described upper lid is provided with the snap ring of evagination（12）, the medial wall of described amplification pipe upper end is provided with and snap ring
The draw-in groove matching（31）, described amplification bottom of the tube center is provided with recessed nucleic acid amplification agents storage part（32）.
2. a kind of visualization sealing nucleic acid amplification according to claim 1 and detection paper device it is characterised in that：Institute
State lid and include top cover（13）And lower extending part（14）, more than lower diameter, lower extending part is in integrally to be inverted to the upper diameter of lower extending part
Convex.
3. a kind of visualization sealing nucleic acid amplification according to claim 2 and detection paper device it is characterised in that：Institute
State snap ring and include snap ring and lower snap ring, upper snap ring is located at the top of lower extending part, lower snap ring is located at the bottom of lower extending part.
4. a kind of visualization sealing nucleic acid amplification according to claim 1 and detection paper device it is characterised in that：Institute
State amplification pipe and include pipe portion（33）And down leg（34）, upper pipe portion with diameter greater than down leg, described nucleic acid amplification agents are deposited
Portion of putting is connected with the bottom of down leg.
5. a kind of visualization sealing nucleic acid amplification according to claim 4 and detection paper device it is characterised in that：Institute
State draw-in groove and include draw-in groove, middle draw-in groove and lower bayonet slot, upper draw-in groove is located at the upper end of upper pipe portion, middle draw-in groove is located under upper pipe portion
End, lower bayonet slot is located at the upper end of down leg.
6. a kind of visualization sealing nucleic acid amplification according to claim 1 or 4 and detection paper device, its feature exists
In：Described clamping part is made up of two panels Plastic clamping piece, and the gap of two panels Plastic clamping piece is 1mm.
7. a kind of visualization sealing nucleic acid amplification according to claim 1 or 4 and detection paper device, its feature exists
In：A diameter of 6-7mm of described nucleic acid amplification agents storage part, depth is 5mm.
8. a kind of visualization sealing nucleic acid amplification according to claim 3 or 5 and detection paper device, its feature exists
In：When upper snap ring is engaged with upper draw-in groove, the bottom of nucleic acid detection test strip is flushed with the top of nucleic acid amplification agents storage part.
9. a kind of visualization sealing nucleic acid amplification according to claim 3 or 5 and detection paper device, its feature exists
In：Engage with middle draw-in groove when upper snap ring, when lower snap ring is engaged with lower bayonet slot, the absorbent section of nucleic acid detection test strip expands in nucleic acid
Increase in reagent storage portion.
10. a kind of visualization sealing nucleic acid amplification according to claim 1 or 4 and detection paper device, its feature exists
In：Described amplification pipe is made up of transparent polystyrene plastics or polyester plasticses.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620979560.0U CN205974498U (en) | 2016-08-29 | 2016-08-29 | Visual leakproofness nucleic acid amplifys and test paper detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620979560.0U CN205974498U (en) | 2016-08-29 | 2016-08-29 | Visual leakproofness nucleic acid amplifys and test paper detection device |
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| CN205974498U true CN205974498U (en) | 2017-02-22 |
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| CN201620979560.0U Active CN205974498U (en) | 2016-08-29 | 2016-08-29 | Visual leakproofness nucleic acid amplifys and test paper detection device |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107964504A (en) * | 2017-10-28 | 2018-04-27 | 深圳职业技术学院 | A kind of closed sample integrity detection device and detection method |
| CN113604346A (en) * | 2021-09-02 | 2021-11-05 | 浙江仅一医疗科技有限公司 | Detection device with reagent and method for detecting nucleic acid by using detection device |
| CN115322889A (en) * | 2022-10-17 | 2022-11-11 | 南京迪飞医疗器械有限公司 | Multi-section solvent reaction tube for biological nucleic acid detection and detection method thereof |
-
2016
- 2016-08-29 CN CN201620979560.0U patent/CN205974498U/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107964504A (en) * | 2017-10-28 | 2018-04-27 | 深圳职业技术学院 | A kind of closed sample integrity detection device and detection method |
| CN107964504B (en) * | 2017-10-28 | 2023-10-27 | 深圳职业技术学院 | Closed sample integrated detection device and detection method |
| CN113604346A (en) * | 2021-09-02 | 2021-11-05 | 浙江仅一医疗科技有限公司 | Detection device with reagent and method for detecting nucleic acid by using detection device |
| CN113604346B (en) * | 2021-09-02 | 2023-11-17 | 浙江仅一医疗科技有限公司 | Detection device containing reagent and method for detecting nucleic acid using detection device |
| CN115322889A (en) * | 2022-10-17 | 2022-11-11 | 南京迪飞医疗器械有限公司 | Multi-section solvent reaction tube for biological nucleic acid detection and detection method thereof |
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