CN107656050B - Blood sample immunochromatography diagnosis test paper and kit - Google Patents
Blood sample immunochromatography diagnosis test paper and kit Download PDFInfo
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- CN107656050B CN107656050B CN201710926294.4A CN201710926294A CN107656050B CN 107656050 B CN107656050 B CN 107656050B CN 201710926294 A CN201710926294 A CN 201710926294A CN 107656050 B CN107656050 B CN 107656050B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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Abstract
A kind of blood sample immunochromatography diagnosis test paper and kit, comprising: substrate；Sample pad, for inputting detection blood sample；The colloidal gold pad is set to the end of the sample pad, antigen or antibody with colloid gold label；Nitrocellulose membrane is arranged detection line and nature controlling line, is used to indicate the testing result of blood sample；First water absorption pad, is set to the other side of substrate, and end and nitrocellulose membrane overlap；Second water absorption pad is set between the first water absorption pad and the substrate；Waterproof isolation film is set between the first water absorption pad and the second water absorption pad, can be extracted out from test strips.Blood sample immunochromatography diagnosis test paper through the invention can accelerate immunochromatography diagnosis speed, while improve detection sensitivity.
Description
Technical field
The present invention relates to immunoassays, more particularly, to a kind of blood sample immunochromatography diagnosis test paper and reagent
Box.
Background technique
Immunolabelling technique refers to fluorescein, radioactive isotope, enzyme, ferritin, colloidal gold and chemistry (or biology) hair
Photo etching etc. is used as the antigen-antibody reaction of tracer, labelled antibody or antigen progress, and by means of fluorescence microscope, radionetric survey
The precision instruments such as instrument, enzyme mark detector, electron microscope and luminescent immunoassay instrument, using various liquid phases and solid-phase immunoassay
Method carries out qualitative and quantitative determination method to haptens, antigen or the antibody in body fluid.
Immunochromatography technique basic principle is exactly to migrate measuring samples solution on chromatography strip, migration
Coated detectable substance (antigen or antibody) carries out affine specific recognition to measuring samples on chromatographic material in the process, carries out immune knot
It closes, object to be checked is made to be enriched in the specific region of chromatography strip, then pass through enzyme reaction or intuitive chromogenic label (such as colloidal gold)
And macroscopic reaction compound is obtained, completely, residual ionization marker can cross detection zone, most for detectable substance consumption to be coated with
After migrate to quality control region and form intuitive colour developing with immune combine of another detectable substance again.
Colloidal gold immunochromatographimethod be built upon enzyme-linked immunosorbent assay, latex agglutination test, monoclonal antibody technique and
In immuno-gold labeling technical foundation using colloidal gold as marker, using special antigen-antibody reaction come iodine,
By directly observing the new technology it is determined that result.It is simple, quick, accurate and pollution-free that the technology has many advantages, such as,
Clinical medicine detection, hormone test, food safety detection, medicament residue and drugs quickly detect many diagnostic fields and send out rapidly
Exhibition.
Colloidal gold immunochromatographimethod diagnosis test paper is according to a kind of detection instrument made of colloidal gold immunochromatographimethod technology, no
Directly pass through naked eyes judging result by professional detecting instrument, can determine whether according to color negative or positive.In the prior art, glue
Body gold immunochromatography diagnosis test paper generallys use the mode unidirectionally chromatographed, and sample arrives water absorption pad along water absorption pad direction, chromatography
In the process and antigen-antibody reaction, presentation T line, C line, unreacted substance continue chromatography to blotting paper due to water sorption.It is single
Shorter to the chromatography reaction time, detection sensitivity is lower.
201110225454.5 patent of invention propose a kind of reciprocation type immunochromatography diagnosis test paper, by sample
Product entrance side setting by can be extracted out waterproof isolation film be isolated blotting paper, chromatograph in test sample to nature controlling line, by waterproof every
Extracted out from film, sample reversely chromatographed, the antigen or antibody in sample on response line antibody or antigen it is anti-again
It answers, to increase the sensitivity of detection.
It, only can be in the sample inlet of test paper but in order to enable sample reversely to chromatograph in 201110225454.5 inventions
Blotting paper is arranged in side, is unable to that blotting paper is arranged in the other side relative to sample inlet side of test paper, causes sample just
It is slower to speed when chromatographing, cause detection time to spend longer；Meanwhile sample is in reversed chromatography, by again with nature controlling line
Antigen-antibody reaction causes reversely to chromatograph to the sample on response line and reduce.
Summary of the invention
The present invention is cited in full text as 201110225454.5 improvement in this, is proposed a kind of blood sample and is exempted from
Epidemic disease chromatographs diagnosis test paper and kit, is able to solve the above problem of the prior art.
As one aspect of the present invention, a kind of blood sample immunochromatography diagnosis test paper is provided, comprising: substrate, sample
Product pad, colloidal gold pad, nitrocellulose membrane and the first water absorption pad；The sample pad is for inputting detection blood sample, setting
In substrate side；The colloidal gold pad is set to the end of the sample end, antigen or antibody with colloid gold label；Nitre
Sour tunica fibrosa is set to one end of the colloidal gold pad, detection line and nature controlling line is arranged, respectively coated antibody or antigen,
It is used to indicate the testing result of blood sample；First water absorption pad, is set to the other side of substrate, and end is taken with nitrocellulose membrane
It connects；It further include the second water absorption pad and waterproof isolation film；Second water absorption pad is set to first water absorption pad and the base
Between bottom, the other end opposite with the one end in the base of nitrocellulose membrane is extended to from one end of substrate；The anti-water segregation
Film is on-fixed isolation film, can be extracted out from test strips, be set to first water absorption pad and second water absorption pad it
Between, the length of slightly longer than described second water absorption pad, the waterproof isolation film described will be prevented after the nature controlling line colour developing when detection
Water segregation film is extracted out from test strips.
Preferably, the liquid absorption amount of second water absorption pad is greater than the absorption liquid measure of the first water absorption pad.
As another aspect of the present invention, a kind of blood sample immunochromatography diagnosis test paper is provided, comprising: base
Bottom, sample pad, colloidal gold pad, nitrocellulose membrane and the first water absorption pad；The sample pad detects blood sample for inputting,
It is set to substrate side；The colloidal gold pad is set to the end of the sample end, the antigen or anti-with colloid gold label
Body；Nitrocellulose membrane, is set to one end of the colloidal gold pad, and detection line and nature controlling line is arranged, respectively coated antibody or
Antigen is used to indicate the testing result of blood sample；First water absorption pad, is set to the other side of substrate, and end and nitric acid are fine
Tie up film overlap joint；Further include: the second water absorption pad, the first waterproof isolation film and the second waterproof isolation film；Second water absorption pad is set
It is placed between first water absorption pad and the substrate, extends to the slightly longer than detection line of nitrocellulose membrane from one end of substrate
Position；The first waterproof isolation film is on-fixed isolation film, can be extracted out from test strips, and first water suction is set to
Between pad and second water absorption pad, the length in substrate is slightly longer than the length of first water absorption pad；Second waterproof
Isolation film is fixed isolation film, is set between second water absorption pad and the nitrocellulose membrane, open region is arranged thereon
Domain, the open area correspond to detection line position；Drawable isolation membrane component is set on the open area.
Preferably, when detection, the first waterproof isolation film is after nature controlling line colour developing, by the described first anti-water segregation
Film is extracted out from test strips.
Preferably, the liquid absorption amount of second water absorption pad is greater than the absorption liquid measure of the first water absorption pad.
Preferably, the drawable isolation membrane component can be extracted out from horizontal plane perpendicular to the first waterproof isolation film
It extracts out in the direction in direction.
As another aspect of the present invention, a kind of detection side of blood sample immunochromatography diagnosis test paper is provided
Method includes the following steps: that blood sample is loaded onto sample pad by (1)；(2) sample passes through colloidal gold pad, quilt after nitrocellulose membrane
It is adsorbed onto the first water absorption pad；(3) after nature controlling line colour developing, the first waterproof isolation film is extracted out from test strips；(4) sample is from
One water absorption pad is further discharged into the second water absorption pad；(5) drawable isolation membrane component is detached from test strips；(6) observation detection
The color of line and nature controlling line determines the testing result of blood sample.
As another aspect of the present invention, a kind of blood sample immunochromatography diagnostic kit is provided comprising on
State blood sample immunochromatography diagnosis test paper.
Detailed description of the invention
Fig. 1 is the blood sample immunochromatography diagnosis test paper of one embodiment of the invention.
Fig. 2 is the blood sample immunochromatography diagnosis test paper of a preferred embodiment of the invention.
Specific embodiment
In order to illustrate more clearly of technical solution of the present invention, embodiment will be used simply to be situated between the present invention below
Continue, it should be apparent that, be described below in be only one embodiment of the present of invention, for those of ordinary skill in the art come
It says, without any creative labor, other technical solutions can also be obtained according to these embodiments, also belonged to
Disclosure of the invention range.
First embodiment
The blood sample immunochromatography diagnosis test paper of first embodiment of the invention, referring to Fig. 1, including substrate 10, sample
Pad 20, colloidal gold pad 30, nitrocellulose membrane 40, the first water absorption pad 50, waterproof isolation film 60 and the second water absorption pad 70.Substrate 10
Substrate 10 is prepared for such as PVC in plastics bonding sheet, can be used.Each composition of blood sample immunochromatography diagnosis test paper
Part or all of position is close on the adhesive surface of substrate 10.
Sample pad 20 is set to one end of substrate 10, and sample input port is arranged thereon, for inputting detection blood sample.Blood
Liquid sample can be whole blood, serum, blood plasma etc..Sample pad 20 can be such as glass fibre, cellulose, poly terephthalic acid
The preparation of the materials such as glycol ester, polyurethane, poly-vinegar acid esters, cellulose acetate, nitrocellulose, nylon, cotton.
Colloidal gold pad 30 is set to the end of sample pad 20, is made of glass fibre.Colloidal gold pad 30 has colloidal gold mark
The antibody or antigen for analyte of note.
Nitrocellulose membrane 40 is set to one end of colloidal gold pad 30, and the blood sample for will test is chromatographed.Nitre
Upper detection line 41 and nature controlling line 42 is arranged in sour tunica fibrosa 40, and coated antibody or antigen, are used to indicate the inspection of blood sample respectively
Survey result.
First water absorption pad 50, is set to the other side of substrate 10, and end and nitrocellulose membrane 40 overlap.First water absorption pad
50 can be used blotting paper preparation, for guiding sample in the chromatography of nitrocellulose membrane 40.
Second water absorption pad 70 is set between the first water absorption pad 50 and substrate 10, extends to nitric acid fibre from one end of substrate 10
Tie up the other end opposite with one end of the substrate 10 of film 40.Second absorption pad 70 in blood sample for chromatographing to nature controlling line 42
Afterwards, it is reversely chromatographed.Material identical with the first water absorption pad 50 can be used in second water absorption pad 70.Preferably, second
The material that water absorbing capacity is greater than the first water absorption pad 50 can be used in water absorption pad 70.
It is set between the first water absorption pad 50 and the second water absorption pad 60 waterproof isolation film 60, and one end stretches out substrate 10
Outside, the length of the other end on the substrate 10 is slightly longer than the length of the second water absorption pad 70.Waterproof isolation film 60 is on-fixed isolation film,
It can be extracted out from test strips, when detection chromatographs in blood sample to nature controlling line 42, by the first waterproof isolation film 60 from test paper
It is extracted out in item, to make sample reverse movement to detection line 41.
The detection method of the blood sample immunochromatography diagnosis test paper of the present embodiment, includes the following steps: (1) by blood
Sample pipetting volume is in sample pad；(2) sample passes through colloidal gold pad, is adsorbed to the first water absorption pad after nitrocellulose membrane；(3) in Quality Control
After line colour developing, waterproof isolation film is extracted out from test strips；(4) sample is further discharged into the second water suction from the first water absorption pad
Pad；(5) color for observing detection line and nature controlling line, determines the testing result of blood sample.
Above-described embodiment through the invention can make the blood sample immunochromatography diagnosis test paper of two-way flow, just
To can also be guided by blotting paper when flowing, to accelerate the detection speed of blood sample immunochromatography diagnosis test paper.
Second embodiment
The preferred embodiment of the invention, referring to fig. 2, including substrate 10, sample pad 20, colloidal gold pad 30, nitrocellulose membrane
40, the first water absorption pad 50, the first waterproof isolation film 60, the second water absorption pad 70 and the second waterproof isolation film 80.
Substrate 10 is that such as PVC preparation substrate 10 can be used in plastics bonding sheet.The diagnosis of blood sample immunochromatography
Part or all of each constituting parts of test strips is close on the adhesive surface of substrate 10.
Sample pad 20 is set to one end of substrate 10, and sample input port is arranged thereon, for inputting detection blood sample.Blood
Liquid sample can be whole blood, serum, blood plasma etc..Sample pad 20 can be such as glass fibre, cellulose, poly terephthalic acid
The preparation of the materials such as glycol ester, polyurethane, poly-vinegar acid esters, cellulose acetate, nitrocellulose, nylon, cotton.
Colloidal gold pad 30 is set to the end of sample pad 20, is made of glass fibre.Colloidal gold pad 30 has colloidal gold mark
The antibody or antigen for analyte of note.
Nitrocellulose membrane 40 is set to one end of colloidal gold pad 30, and the blood sample for will test is chromatographed.Nitre
Upper detection line 41 and nature controlling line 42 is arranged in sour tunica fibrosa 40, and coated antibody or antigen, are used to indicate the inspection of blood sample respectively
Survey result.
First water absorption pad 50, is set to the other side of substrate 10, and end and nitrocellulose membrane 40 overlap.First water absorption pad
50 can be used blotting paper preparation, for guiding sample in the chromatography of nitrocellulose membrane 40.
Second water absorption pad 70 is set between the first water absorption pad 50 and substrate 10, is extended to slightly longer than from one end of substrate 10
The position of the detection line 41 of nitrocellulose membrane 40.Second absorption pad 70 in blood sample for chromatographing to nature controlling line 42, by it
Reversely chromatographed.Material identical with the first water absorption pad 50 can be used in second water absorption pad 70.Preferably, the second water absorption pad 70
The material that water absorbing capacity is greater than the first water absorption pad 50 can be used.
It is set between the first water absorption pad 50 and the second water absorption pad 60 first waterproof isolation film 60, and one end stretches out substrate
Outside 10, the length of the other end on the substrate 10 is slightly longer than the length of the first water absorption pad 50.Waterproof isolation film 60 is on-fixed isolation
Film can be extracted out from test strips, to make sample reverse movement to detection line 41.
Second waterproof isolation film 80 is fixed isolation film, is disposed adjacent in the first waterproof isolation film 60, is located at second and inhales
Between water cushion 70 and nitrocellulose membrane 40.The length that second waterproof isolation film 80 extends on the substrate 10 is slightly longer than the second water absorption pad
70。
Open area 81 is set on second waterproof isolation film 80, and open area 81 corresponds to the position of detection line 41.Opening
Drawable isolation membrane component 82 is set on region 81.Drawable isolation membrane component 82 can be from horizontal plane perpendicular to the first waterproof
It extracts out in the direction of 60 extraction direction of isolation film.
The detection method of the blood sample immunochromatography diagnosis test paper of the present embodiment, includes the following steps: (1) by blood
Sample pipetting volume is in sample pad；(2) sample passes through colloidal gold pad, is adsorbed to the first water absorption pad after nitrocellulose membrane；(3) in Quality Control
After line colour developing, the first waterproof isolation film is extracted out from test strips；(4) sample is further discharged into the second suction from the first water absorption pad
Water cushion；(5) drawable isolation membrane component is detached from test strips；(6) color for observing detection line and nature controlling line, determines blood
The testing result of sample.
Setting in preferred embodiment through the invention, on the one hand, blood sample immunochromatography diagnosis test paper can be accelerated
On the other hand the detection speed of item makes sample in reverse flow, no longer with the antigen-antibody reaction of nature controlling line, further mentions
High detection sensitivity.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the scope of the present invention.This hair
Relative size in bright attached drawing needs and is arranged merely to showing, is not specifically limited it.Such as prevent in the present invention
The thickness of water segregation film can be very thin relative to other elements.Specific features described in the present invention, structure, material
Or feature may be combined in any suitable manner in any one or more of the embodiments or examples.In addition, not conflicting
In the case of, those skilled in the art can by different embodiments or examples described in this specification and different embodiment or
Exemplary feature is combined.Any modification done within the spirit and principles of the present invention, changes equivalent replacement
Into etc., it is included within the scope of protection of the present invention.
Claims (4)
1. a kind of blood sample immunochromatography diagnosis test paper, comprising: substrate, sample pad, colloidal gold pad, nitrocellulose membrane and
First water absorption pad；The sample pad is set to substrate side for inputting detection blood sample；The colloidal gold pad is set to
The end of the sample pad, antigen or antibody with colloid gold label；Nitrocellulose membrane is set to the colloidal gold pad
One end, detection line and nature controlling line are set, and coated antibody or antigen, are used to indicate the testing result of blood sample respectively；The
One water absorption pad, is set to the other side of substrate, and end and nitrocellulose membrane overlap；It is characterized by also including the second water suctions
Pad and waterproof isolation film；Second water absorption pad is set between first water absorption pad and the substrate, from the one of substrate
End extends to the other end opposite with one end of the substrate of nitrocellulose membrane；The waterproof isolation film is on-fixed isolation film,
It can extract out, be set between first water absorption pad and second water absorption pad, slightly longer than described second from test strips
The length of water absorption pad, when detection the waterproof isolation film after nature controlling line colour developing, by the waterproof isolation film from test strips
Middle extraction.
2. blood sample immunochromatography diagnosis test paper according to claim 1, it is characterised in that: second water absorption pad
Liquid absorption amount be greater than the first water absorption pad absorption liquid measure.
3. a kind of detection method of blood sample immunochromatography diagnosis test paper according to claim 1, including walk as follows
It is rapid: (1) blood sample to be loaded onto sample pad；(2) sample passes through colloidal gold pad, is adsorbed to the first water suction after nitrocellulose membrane
Pad；(3) after nature controlling line colour developing, waterproof isolation film is extracted out from test strips；(4) sample is further discharged from the first water absorption pad
To the second water absorption pad；(5) color for observing detection line and nature controlling line, determines the testing result of blood sample.
4. a kind of blood sample immunochromatography diagnostic kit comprising blood sample described in one of claim 1-2 is immune
Chromatograph diagnosis test paper.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710926294.4A CN107656050B (en) | 2017-10-07 | 2017-10-07 | Blood sample immunochromatography diagnosis test paper and kit |
| CN201811410566.6A CN109187962A (en) | 2017-10-07 | 2017-10-07 | Blood sample immunochromatography diagnosis test paper, kit and detection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710926294.4A CN107656050B (en) | 2017-10-07 | 2017-10-07 | Blood sample immunochromatography diagnosis test paper and kit |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811410566.6A Division CN109187962A (en) | 2017-10-07 | 2017-10-07 | Blood sample immunochromatography diagnosis test paper, kit and detection method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107656050A CN107656050A (en) | 2018-02-02 |
| CN107656050B true CN107656050B (en) | 2019-06-04 |
Family
ID=61116405
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811410566.6A Withdrawn CN109187962A (en) | 2017-10-07 | 2017-10-07 | Blood sample immunochromatography diagnosis test paper, kit and detection method |
| CN201710926294.4A Expired - Fee Related CN107656050B (en) | 2017-10-07 | 2017-10-07 | Blood sample immunochromatography diagnosis test paper and kit |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811410566.6A Withdrawn CN109187962A (en) | 2017-10-07 | 2017-10-07 | Blood sample immunochromatography diagnosis test paper, kit and detection method |
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| Country | Link |
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| CN (2) | CN109187962A (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108982495A (en) * | 2018-10-19 | 2018-12-11 | 医芯（厦门）科技有限公司 | Drawing and pulling type detects pen, detection platform and detection method |
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| WO1992021977A1 (en) * | 1991-05-29 | 1992-12-10 | Smithkline Diagnostics, Inc. | Assay device |
| US6007999A (en) * | 1991-07-31 | 1999-12-28 | Idexx Laboratories, Inc. | Reversible flow chromatographic binding assay |
| CN1277357A (en) * | 1999-06-14 | 2000-12-20 | 普莱克斯生物系统股份有限公司 | Two-way sidestream test strip |
| CN1397021A (en) * | 2000-01-28 | 2003-02-12 | 基因实验室诊断私人有限公司 | Immunochromatographic assay devices with separators |
| WO2005069007A1 (en) * | 2004-01-14 | 2005-07-28 | British Biocell International Limited | Improvements in or relating to lateral flow assay devices |
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| CN103743900A (en) * | 2014-01-24 | 2014-04-23 | 厦门为正生物科技有限公司 | Immunochromatography detection device and method by adopting two-step method |
| CN205484358U (en) * | 2016-01-20 | 2016-08-17 | 江苏衡谱分析检测技术有限公司 | Combined two -way colloidal gold immunoassay chromatography test paper strip |
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| US5877028A (en) * | 1991-05-29 | 1999-03-02 | Smithkline Diagnostics, Inc. | Immunochromatographic assay device |
| US6767710B2 (en) * | 2001-03-30 | 2004-07-27 | Praxsys Biosystems, Llc | Prewetting stop flow test strip |
| EP1787121B1 (en) * | 2004-07-29 | 2010-08-25 | Relia Diagnostic Systems, LLC | Lateral flow system and assay |
| US20060068501A1 (en) * | 2004-09-30 | 2006-03-30 | Nj International Llc | Apparatus and methods for steroid hormone testing |
| CN1786712A (en) * | 2004-12-07 | 2006-06-14 | 盛进生物工程(深圳)有限公司 | Method and system for quantitatively fast level testing flow |
| CN203688562U (en) * | 2014-01-24 | 2014-07-02 | 厦门为正生物科技有限公司 | Two-step method immunochromatography testing device |
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2017
- 2017-10-07 CN CN201811410566.6A patent/CN109187962A/en not_active Withdrawn
- 2017-10-07 CN CN201710926294.4A patent/CN107656050B/en not_active Expired - Fee Related
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1992021977A1 (en) * | 1991-05-29 | 1992-12-10 | Smithkline Diagnostics, Inc. | Assay device |
| US6007999A (en) * | 1991-07-31 | 1999-12-28 | Idexx Laboratories, Inc. | Reversible flow chromatographic binding assay |
| CN1277357A (en) * | 1999-06-14 | 2000-12-20 | 普莱克斯生物系统股份有限公司 | Two-way sidestream test strip |
| CN1397021A (en) * | 2000-01-28 | 2003-02-12 | 基因实验室诊断私人有限公司 | Immunochromatographic assay devices with separators |
| WO2005069007A1 (en) * | 2004-01-14 | 2005-07-28 | British Biocell International Limited | Improvements in or relating to lateral flow assay devices |
| CN102778558A (en) * | 2011-05-10 | 2012-11-14 | 北京伊康纳斯生物医药科技有限公司 | Lateral reciprocating immunochromatography method and diagnosis test strip and device based on lateral reciprocating immunochromatography method |
| CN103743900A (en) * | 2014-01-24 | 2014-04-23 | 厦门为正生物科技有限公司 | Immunochromatography detection device and method by adopting two-step method |
| CN205484358U (en) * | 2016-01-20 | 2016-08-17 | 江苏衡谱分析检测技术有限公司 | Combined two -way colloidal gold immunoassay chromatography test paper strip |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107656050A (en) | 2018-02-02 |
| CN109187962A (en) | 2019-01-11 |
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