CN207502542U - A kind of detection kit for colloidal gold method - Google Patents
A kind of detection kit for colloidal gold method Download PDFInfo
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- CN207502542U CN207502542U CN201721287625.6U CN201721287625U CN207502542U CN 207502542 U CN207502542 U CN 207502542U CN 201721287625 U CN201721287625 U CN 201721287625U CN 207502542 U CN207502542 U CN 207502542U
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- colloidal gold
- groove body
- upper cartridge
- detection
- lower box
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Abstract
The utility model discloses a kind of detection kits for colloidal gold method, including lower box body, the upper cartridge body being fixedly connected with the lower box body, it is opened in the well and the first observation window of the upper surface of upper cartridge body successively, the inside of the lower box body from left to right offers the first groove body successively, third groove body for the second groove body for placing chemical test paper and for placing immune detection card, first partition is provided between first groove body and the second groove body, second partition is provided between second groove body and third groove body, the second partition is extended with and the integrally formed slope of the second partition towards the third groove body.Adhesive layer is posted on inclined-plane described in the utility model, the PVC board of the bottom of the immune detection card is bonded and fixed on the inclined-plane, it is placed since the immune detection is stuck in the third groove body inner inclination, it can promote to detect liquid (such as blood of human body) quickening chromatography, and then improve the detection rates of colloidal gold chromatography.
Description
Technical field
The utility model belongs to immunology applied technical field, and in particular to a kind of detection reagent for colloidal gold method
Box.
Background technology
The detection method of hepatopathy, some gastrointestinal diseases and malignant tumour etc. mainly has immunoturbidimetry, enzyme to be immunized point at present
Analysis method, radio immunoassay (Radioimmunoassay, RIA), Electrochemiluminescince and it is suitble to application in emergency treatment
The other detection (such as colloidal gold method, fluorescence immune chromatography method) of bed.
Immunoturbidimetry：When antigen and antibody react and suitable (the general provision antibody of ratio in special dilution system
It is excessive) when, under the action of the poly- agent of rush (polyethylene glycol etc.) of the soluble immune complex of formation in dilution system, from liquid phase
It is precipitated, forms particle, reaction solution is made turbidity occur.When antibody concentration is fixed, the amount of the immune complex of formation is with sample
The increase of middle amount of antigen and increase, the turbidity of reaction solution is consequently increased.By the turbidity and series of standards that measure reaction solution
Product compare, you can calculate the content of antigen in sample.Immunoturbidimetry analysis carries out on full-automatic immunoturbidimetry analyzer,
High degree of automation suitable for the processing of clinical high-volume sample, but cannot be used for the detection of whole blood sample.
Noncompetitive enzyme immunoassay (EIA)：Microwell plate is coated with the specific antibody antibody of purifying, solid phase antibody is made, toward packet
Specific antibody antigen, then the specific antibody antibody knot with horseradish peroxidase HRP labels are sequentially added in the micropore of monoclonal antibody
It closes, forms antibody-antigene-hrp-antibody complex, after thoroughly washing plus substrate TMB develops the color.TMB is in the catalysis of HRP enzymes
Lower conversion au bleu, and it is converted to final yellow under the action of an acid.Specific antibody antigen in the depth and sample of color
It is proportionate.Enzyme immunoassay (EIA) measure takes 45min, takes longer, unsuitable clinical practice.
Radio immunoassay：Radioimmunoassay technique is the principle with competitive binding, utilizes radioactive isotope
(Ag*) and non-labeled antigen (Ag) is marked to react the competitive binding of specific antibody (Ab), is combined by measuring Ag-Ab
The radioactivity of object judges the amount of non-labeled antigen.Radiommunoassay has radiocontamination, radionuclide decay and not
The shortcomings that stablizing.
Chemiluminescence immunoassay：It will be with the immune anti-of highly sensitive chemical luminescent detecting technology and high specific
The technology that should be combined is improved on the basis of radiommunoassay and ELISA.It is pressed from both sides using one step of double antibody
Heart EIA enzyme immunoassay using chemiluminescent agent as substrate, is solid phase carrier with monoclonal antibody coating magnetic particle, increases absorption
Surface area can detach with liquid in magnetic field, simplify operating procedure.Electrochemiluminescince detection range is wide, precision is high, steady
It is qualitative good, but need expensive instrument.The other detection of bed is then easy to operate, detection time is short, result is also relatively reliable.
Immune colloidal gold technique (Immune colloidal gold technique) is using colloidal gold as tracer label
Object is applied to a kind of novel immunolabelling technique of antigen-antibody, and english abbreviation is：GICT.The technical operation is easy, detection
Quickly, outside the advantages of portability is strong, the accurate quantification of testing result is realized.
Immunochromatography colloidal gold technique is novel and relatively simple diagnostic techniques, is quite widely applied, base
Present principles are：Using a kind of antigen of colloid gold label or antibody, corresponding pairing antigen or antibody are coated on the NC films of reagent,
During detection when containing corresponding specific antibody or antigen in sample, ligand is combined to be formed in colloid gold label particle and sample
Then compound chromatographs on NC films, then coated antigen or antibody paving are obtained, and form macroscopic detection (T) line, pass through inspection
The presence or absence of survey line realizes the judgement to result.With easy to operate, rapid reaction, sensibility height, high specificity, it is suitble to scene inspection
The advantages that surveying and be economical and practical.
Existing immune test paper is typically all to lie in immunity detection reagent, and detection liquid is in the immune test paper
The speed of upper chromatography is slower, and in detection applied technical field on a large scale, this often makes troubles to detection, meanwhile, it is existing
Detection kit is inconvenient to hold, also without moisture-proof effect.
Utility model content
The utility model devises a kind of detection kit for immunochromatography colloidal gold method to solve the above-mentioned problems,
The detection kit have easy to operate, rapid reaction, sensibility height, high specificity, convenient for it is hand-held, be suitble to scene on a large scale
The advantages that detecting and be economical and practical.
The purpose of this utility model is achieved through the following technical solutions：
A kind of detection kit for colloidal gold method, including lower box body, the upper cartridge body being fixedly connected with the lower box body,
The upper surface of the upper cartridge body is equipped with well and the first observation window, and the inside of the lower box body from left to right offers the successively
One groove body, the third groove body for the second groove body for placing chemical test paper and for placing immune detection card, the immune inspection
It surveys card and is equipped with detection line and nature controlling line, first partition, second slot are provided between first groove body and the second groove body
Second partition is provided between body and third groove body, the slope of the third groove body extension is provided on the second partition.
Further, the immune detection card is fixed on the inclined-plane on the slope, when the lower box body and the upper box
When body is fixedly connected, first observation window is located exactly at the top of the detection line and nature controlling line.
Further, it offers to observe the chemistry examination on the upper surface of the upper cartridge body above the second groove body
Second observation window of paper color.
Further, when the lower box body is fixedly connected with the upper cartridge body, the second partition is located exactly at described
The lower section at the middle part of well, close to the equal position of left end of the right end and immune detection card of the chemical test paper of the second partition
In the underface of the well.
Further, it is posted on the bottom surface of second groove body and the inclined-plane on the slope and is respectively used to bond describedization
Learn the adhesive layer of test paper and immune detection card.
Further, the right side of the upper cartridge body extends to form to the right lug, and the upper surface of the lug offers recessed
Slot, is neatly provided with the anti-skidding salient point of multiple upper surface flush with the upper cartridge body in the groove, the lug, groove and
Anti-skidding salient point together constitutes the grip part of the detection kit.
Further, the left end of the upper surface of the upper cartridge body is equipped with label adhering part.
Further, the label adhering part label and/or Quick Response Code.
Further, the lower box body is snapped connection with the upper cartridge body.
Further, the inner wall of opposite two side of the lower box body has been respectively and fixedly connected with multiple jacks, the upper box
The inner wall of corresponding two sides of body has been respectively fixedly connected with multiple slotting axis, and the upper cartridge body is with the lower box body by inserting
The mode that axis is inserted into jack snaps connection together.
Compared with prior art, the utility model has at least the following advantages：
1st, the second partition of the detection kit of the colloidal gold method is extended with and described second towards the third groove body
Adhesive layer is posted on the inclined-plane in partition board integrally formed slope, and the PVC board of the bottom of the immune detection card is bonded and fixed at
On the inclined-plane, placed since the immune detection is stuck in the third groove body inner inclination, can promote to detect liquid (such as people
Body blood etc.) accelerate chromatography, and then improve the detection rates of colloidal gold chromatography.
2nd, chemical test paper is provided in second groove, the chemistry test paper receives simultaneously with the immune detection card
The inspection of antigenic substance, it is described chemistry test paper testing result whether the testing result of the immune detection card is accurately played it is auxiliary
Card acts on.
3rd, the right side of the upper cartridge body of the detection kit of the colloidal gold method forms lug to the right, the lug it is upper
Side face opens up fluted, and the anti-skidding salient point of multiple upper surface flush with the upper cartridge body, institute are neatly provided in the groove
State the grip part that lug, groove and anti-skidding salient point together constitute the detection kit.The lug is convenient for staff's hand
It holds, the anti-skidding salient point can be semicircle or triangle, play anti-skidding effect.
4th, the left end of the upper surface of the upper cartridge body of the colloidal gold method is equipped with label adhering part, and the label adhering part posts
Label and/or Quick Response Code.The label or Quick Response Code, which are spent, distinguishes different labels, and the information of especially Quick Response Code is held
Carrying capacity is big, and available for recording other relevant informations of the detection kit, the detection kit is convenient for detecting on a large scale
Middle use
Description of the drawings
Fig. 1 is the structure diagram of the upper cartridge body of detection kit described in the utility model；
Fig. 2 is the structure diagram of the lower box body of detection kit described in the utility model；
Fig. 3 is the structure diagram of immune detection card described in the utility model.
Wherein, 1- upper cartridge bodies；2- lower box bodies；The first observation windows of 3-；4- wells；The second observation windows of 5-；6- labels paste
Portion；7- lugs；8- grooves；9- anti-skidding salient points；10- first partitions；11- second partitions；12- slopes；13- jacks；14-PVC plates；
15- sample pads；16- detection lines；17- nature controlling lines.
Specific embodiment
The utility model is described in further detail with reference to the accompanying drawings and examples, following embodiment be it is descriptive,
It is not limited, it is impossible to which the scope of protection of the utility model is limited with this.
A kind of detection kit for colloidal gold method, the upper box being fixedly connected including lower box body 2, with the lower box body 2
Body 1, the two can form the accommodation space for accommodating chemical test paper and immune detection card, be opened in institute successively between being fixedly connected afterwards
4 and first observation window 3 of well of the upper surface of upper cartridge body 1 is stated, the inside of the lower box body 2 from left to right offers successively
One groove body, the third groove body for the second groove body for placing chemical test paper and for placing immune detection card, first slot
First partition 10 is provided between body and the second groove body, second partition 11 is provided between second groove body and third groove body,
The second partition 11 is extended with and the 11 integrally formed slope 12 of second partition towards the third groove body.
It in the present embodiment, is posted on the inclined-plane viscous for fixing the immune detection card on the inclined-plane on the slope 12
Layer is tied, the PVC board 14 of the bottom of the immune detection card is bonded and fixed on the inclined-plane, and the immune detection card is equipped with inspection
Survey line and nature controlling line are placed since the immune detection is stuck in the third groove body inner inclination, can promote to detect liquid (such as
Blood of human body etc.) accelerate chromatography, and then improve the detection rates of colloidal gold chromatography.
When it is implemented, be fixed with the immune detection card on the inclined-plane on the slope 12, when the lower box body 2 with it is described
When upper cartridge body 1 is fixedly connected, first observation window 3 is located exactly at the detection line 16 of the immune detection card and nature controlling line 17
Top.Testing staff judges patient's illness by the discoloration of detection line 16 and nature controlling line 17.
When it is implemented, it is further opened with seeing positioned at the upper surface of the upper cartridge body 1 on the top of second groove body
The second observation window 5 of the chemical test paper color is examined, the bottom surface of second groove body posts to bond the chemical test paper
Adhesive layer, the chemistry test paper are pasted on the adhesive layer.
When it is implemented, when the lower box body 2 is fixedly connected with the upper cartridge body 1, the 11 lucky position of second partition
In the lower section at the middle part of the well 4, close to the chemical right end of test paper and immune detection card of the second partition 11
Left end be respectively positioned on the underface of the well.After manually more drop detection liquid are instilled by well 4, liquid is detected in part
Body is in contact with the chemical test paper, and other part detection liquid is in contact simultaneously quick with the sample pad 15 of the immune detection card
Infiltration, about 15min or so are judged by observing the color change of detection line 16 on immune detection card and nature controlling line 17
Testing result, while 10% hydrogenperoxide steam generator is added dropwise to the chemical test paper, the color change of the chemical test paper is observed,
Final testing result is judged finally by the result of two kinds of detection methods.
When it is implemented, the right side of the upper cartridge body 1 extends to form lug 7 to the right, the upper surface of the lug 7 is opened
Equipped with groove 8, the anti-skidding salient point 9 of multiple upper surface flush with the upper cartridge body 1 is neatly provided in the groove 8, it is described
Lug 7, groove 8 and anti-skidding salient point 9 together constitute the grip part of the detection kit.The lug 7 is convenient for staff
Hand-held, the anti-skidding salient point 9 can be semicircle or triangle, play anti-skidding effect.
When it is implemented, the left end of the upper surface of the upper cartridge body 1 is equipped with label adhering part 6, the label adhering part 6 pastes
There are label and/or Quick Response Code.The label or Quick Response Code, which are spent, distinguishes different labels, especially the information of Quick Response Code
Bearing capacity is big, and available for recording other relevant informations of the detection kit, the detection kit is convenient for examining on a large scale
It is used in survey.
When it is implemented, the lower box body 2 is snapped connection with the upper cartridge body 1.
When it is implemented, the inner wall of opposite two side of the lower box body 2 has been respectively and fixedly connected with multiple jacks 13, it is described
The inner wall of corresponding two sides of upper cartridge body 1 has been respectively fixedly connected with multiple slotting axis, the upper cartridge body 1 and the lower box body
2 are snapped connection together by way of inserting axis and being inserted into jack 13.
More than, the only preferable specific embodiment of the utility model, but the protection domain of utility model is not limited to
This, in the technical scope that any one skilled in the art discloses in the utility model, the variation that can readily occur in
Or replace, it should be covered within the scope of the utility model.Therefore, the scope of protection of the utility model should be with right
Subject to the protection domain of claim.
Claims (10)
1. a kind of detection kit for colloidal gold method, including lower box body, the upper cartridge body being fixedly connected with the lower box body, institute
State upper cartridge body upper surface be equipped with well and the first observation window, which is characterized in that the inside of the lower box body from left to right according to
It is secondary to offer the first groove body, the third groove body for the second groove body for placing chemical test paper and for placing immune detection card,
The immune detection card is equipped with detection line and nature controlling line, and first partition is provided between first groove body and the second groove body,
Second partition is provided between second groove body and third groove body, the third groove body is provided on the second partition and is prolonged
The slope stretched.
2. the detection kit according to claim 1 for colloidal gold method, which is characterized in that on the inclined-plane on the slope
The immune detection card is fixed with, when the lower box body is fixedly connected with the upper cartridge body, the lucky position of the first observation window
In the detection line and the top of nature controlling line.
3. the detection kit according to claim 1 or 2 for colloidal gold method, which is characterized in that on the second groove body
The second observation window for observing the chemical test paper color is offered on the upper surface of the upper cartridge body of side.
4. the detection kit according to claim 3 for colloidal gold method, which is characterized in that when the lower box body and institute
When stating upper cartridge body and being fixedly connected, the second partition is located exactly at the lower section at the middle part of the well, close to described second every
The chemical right end of test paper and the left end of immune detection card of plate are respectively positioned on the underface of the well.
5. the detection kit according to claim 2 for colloidal gold method, which is characterized in that the bottom of second groove body
The adhesive layer for being respectively used to bond the chemical test paper and immune detection card is posted on the inclined-plane on face and the slope.
6. the detection kit according to claim 1 for colloidal gold method, which is characterized in that the right side of the upper cartridge body
Extend to form lug towards the right side, the upper surface of the lug open up it is fluted, be neatly provided in the groove it is multiple with it is described
The anti-skidding salient point of the upper surface flush of upper cartridge body, the lug, groove and anti-skidding salient point together constitute the detection kit
Grip part.
7. the detection kit according to claim 1 or 2 for colloidal gold method, which is characterized in that the upper cartridge body
The left end of upper surface is equipped with label adhering part.
8. the detection kit according to claim 7 for colloidal gold method, which is characterized in that the label adhering part patch
There are label and/or Quick Response Code.
9. the detection kit according to claim 1 for colloidal gold method, which is characterized in that the lower box body with it is described
Upper cartridge body snaps connection.
10. the detection kit according to claim 9 for colloidal gold method, which is characterized in that the phase of the lower box body
To the inner walls of two sides be respectively and fixedly connected with multiple jacks, the inner wall of corresponding two sides of the upper cartridge body is solid respectively
Surely multiple slotting axis are connected with, the upper cartridge body snaps connection together with the lower box body by way of inserting axis and being inserted into jack.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721287625.6U CN207502542U (en) | 2017-09-30 | 2017-09-30 | A kind of detection kit for colloidal gold method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721287625.6U CN207502542U (en) | 2017-09-30 | 2017-09-30 | A kind of detection kit for colloidal gold method |
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| Publication Number | Publication Date |
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| CN207502542U true CN207502542U (en) | 2018-06-15 |
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| CN201721287625.6U Active CN207502542U (en) | 2017-09-30 | 2017-09-30 | A kind of detection kit for colloidal gold method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109100505A (en) * | 2018-08-22 | 2018-12-28 | 宁波奥丞生物科技有限公司 | A kind of immunofluorescent reagent box of quantitative detection blood vessel endothelium marker CD105 |
| CN113624961A (en) * | 2020-03-03 | 2021-11-09 | 坚石生物科技有限公司 | Lateral flow diagnostic test device |
-
2017
- 2017-09-30 CN CN201721287625.6U patent/CN207502542U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109100505A (en) * | 2018-08-22 | 2018-12-28 | 宁波奥丞生物科技有限公司 | A kind of immunofluorescent reagent box of quantitative detection blood vessel endothelium marker CD105 |
| CN113624961A (en) * | 2020-03-03 | 2021-11-09 | 坚石生物科技有限公司 | Lateral flow diagnostic test device |
| EP3907505A3 (en) * | 2020-03-03 | 2022-01-26 | Pebble-i Inc. | Lateral flow diagnostic testing apparatus |
| US11486832B2 (en) | 2020-03-03 | 2022-11-01 | Pebble-i Inc. | Lateral flow diagnostic testing apparatus |
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