CN205374466U - Ration jointly detects kit of miocardial infarction - Google Patents
Ration jointly detects kit of miocardial infarction Download PDFInfo
- Publication number
- CN205374466U CN205374466U CN201521060175.8U CN201521060175U CN205374466U CN 205374466 U CN205374466 U CN 205374466U CN 201521060175 U CN201521060175 U CN 201521060175U CN 205374466 U CN205374466 U CN 205374466U
- Authority
- CN
- China
- Prior art keywords
- antibody
- myoglobin
- ctni
- fabp
- troponin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The utility model relates to a ration jointly detects kit of miocardial infarction, can while quantitative determination myoglobin (Myo), heart type fatty acid binding protein (h -FABP), four myocardium indexs of creatine kinase isoenzyme (CK -MB) and troponin I (cTnI), contain and detect card and reading appearance, it constitutes with 2 colloidal gold test paper strips arranging inside in wherein to detect the plastic jam who blocks by the outside, article one, be myoglobin (Myo), heart type fatty acid binding protein (h -FABP) test paper strip, another is creatine kinase isoenzyme (CK -MB), troponin I (cTnI) test paper strip. The utility model discloses the kit has and detects fastly, and the operation is succinct, and is with low costs, good reproducibility, characteristics that the suitability is strong.
Description
Technical field
This utility model belongs to associating quick detection reagent field, is specially the test kit of the quantitative joint-detection of a kind of myocardial infarction.
Background technology
Myoglobin (Myo) be a kind of oxygen in conjunction with hemoprotein, be distributed mainly on cardiac muscle and skeletal muscle tissue.When acute myocardial injury, Myoglobin is released in blood at first, occurs that about after 2-3 hour, in blood, Myoglobin can exceed upper limits of normal, within 9-12 hour, reaches peak value in clinical symptoms, recovers normal after 24-36 hour.Measure serum myoglobin and can diagnose index the sensitiveest in early days as acute myocardial infarction (AMI).But its poor specificity, when diseases such as Skeletal muscle injury, wound, renal failures, all may result in it and raises.Though the Myo positive can not make a definite diagnosis AMI, but can as the important indicator of early stage eliminating AMI.
Myocardium enzyme, myocardial damage or after bad plug some enzyme have increasing in various degree, wherein CK-MB specificity is the highest.CK-MB is primarily present in cardiac muscle, within after being typically in myocardial damage 3～8 hours, raises, within 9～30 hours, reaches peak, within 48～72 hours, recovers normal.And its peak time of occurrence has certain relation with prognosis degree, peak occurs that morning person's prognosis is better.The nineties in 20th century CK-MB be considered as diagnosis AMI goldstandard.
Cardic fatty acid binding protein (h-FABP) be in heart rich in the novel little cytoplasmic protein of one.It has height heartspecific (namely main expression in heart tissue).After myocardial ischemic injury occurs, h-FABP can be found for 1-3 hour after episode in blood, within 6-8 hour, reaches peak value, and blood plasma level recovered normal in 24-30 hour.The feature of h-FABP is similar to Myoglobin, but more has heartspecific due to h-FABP, and it is considered as a kind of to myocardial damage diagnosis index more accurately due to its biological characteristics.
The integrity of cardiac muscle cells film is damaged, cTnI first discharges in endochylema, therefore, in serum, cTnI level quickly raises, and the cTnI combined is big due to relative molecular mass, from the release that myocardial ultramicrostructure albumen is slowly lasting, after myocardial damage, 2～8h is released into blood, reaches summit at 12～24h, and high level often maintains 7～10d.Owing to cTnI relatively early occurs in blood, and the persistent period is long, and Sensitivity and Specificity is strong, and the diagnosis for myocardial damage is very valuable.CTnI be unanimously chosen as by US and European heart disease association be the high specific of diagnosing acute myocardial infarction and hypersensitivity make a definite diagnosis mark.
Mainly single Testing index or three link detection reagent kits of current Clinical practice, including Myoglobin, creatine kinase isozyme, individually detection or the joint-detection of troponin, there is an obvious defect in these three detections, Myo can show high sensitivity at the early stage 1-6h that myocardial infarction occurs, but its poor specificity, cTnI high specificity, but the sensitivity within 6h is not high, and add the accuracy that the detection of h-FABP can be improved in 6h diagnosis of myocardial damage, this utility model is detection by quantitative Myoglobin simultaneously, creatine kinase isozyme, cardic fatty acid binding protein, the level of troponin (I), can be more accurate, more comprehensively, judge the degree of myocardial damage more quickly.
Summary of the invention
The purpose of this utility model is in that a kind of quantitative combined detection kit that can be used in acute myocardial infarction diagnosis of exploitation, reaches accurate, to diagnose myocardial infarction comprehensively, rapidly developmental stage.
For achieving the above object, this utility model is by the following technical solutions:
The test kit of a kind of quantitative joint-detection myocardial infarction, comprises detection card and readout instrument, and wherein detection card is got stuck by upper and lower two blocks of plastics and is placed in 2 internal colloidal gold strips and forms.And 2 the structure of colloidal gold strip is identical, as shown in Figure 1.Colloidal gold strip is by sample pad (1), gold-marking binding pad (2), NC film (3), base plate (4), absorbent paper (5) forms, described gold-marking binding pad, NC film, absorbent paper are fixed on below base plate so that horizontal direction is connected in order, and gold-marking binding pad, NC film, absorbent paper adjacent part are respectively arranged with the overlapping region of 1-2mm, and above-mentioned gold-marking binding pad is stained with sample pad.Upper plastics get stuck and are provided with 2 wells (9) and 2 observation windows (10), and lower plastic housing is provided with 2 locating grooves being used for fixing 2 colloidal gold strips.
Described 2 one of them draw-in grooves of locating groove place Myoglobin (Myo) and cardic fatty acid binding protein (h-FABP) colloidal gold strip, another draw-in groove places creatine kinase isozyme (CK-MB) and Troponin I (cTnI) colloidal gold strip, article two, arranged in parallel being placed in of colloidal gold strip is got stuck, and position is as shown in Figure 2.
2 described colloidal gold strips have respectively correspondence well and observation window, and upper plastics get stuck detection line position place indicate each detection purpose title.
From sample pad direction to absorbent paper direction on the NC film of described Myoglobin (Myo) and cardic fatty acid binding protein (h-FABP) colloidal gold strip, be sequentially provided with detection line 1(7), detection line 2(6) and nature controlling line (8).Described detection line 1(7) coated be Myoglobin antibody, detect line 2(6) coated be cardic fatty acid binding protein, nature controlling line (8) is coated is that sheep anti mouse two resists；Myoglobin (Myo) antibody and cardic fatty acid binding protein (h-FABP) antibody being marked with on gold-marking binding pad and detect line pairing.
The detection line 1(7 of the NC film of described creatine kinase isozyme (CK-MB) and Troponin I (cTnI) colloidal gold strip) on be coated creatine kinase isozyme (CK-MB) antibody, detection line 2(6) on be coated Troponin I (cTnI) antibody, nature controlling line (8) is coated sheep anti mouse two and resists；Creatine kinase isozyme (CK-MB) antibody and Troponin I (cTnI) antibody being marked with on gold-marking binding pad and detect line pairing.
The beneficial effects of the utility model are:
1. multinomial detection.This utility model detection kit can simultaneous quantitative detection Myoglobin (Myo), cardic fatty acid binding protein (h-FABP), creatine kinase isozyme (CK-MB), Troponin I (cTnI) four indices level, it is possible to more accurately more fully grasp the development of myocardial infarction.
2. qualitative, quantitative is all applicable.This utility model test kit can qualitatively judge the yin and yang attribute result of four indices, it is possible in conjunction with the level of readout instrument Accurate Determining four indices, applied range, uses more convenient.
3. easy and simple to handle, detection is quickly.This utility model detection kit is applicable to measure serum and blood plasma, and after point sample, sentence read result in 20 minutes, easy and simple to handle, fast.
Accompanying drawing illustrates:
Fig. 1 is the side structure schematic diagram of colloidal gold strip in this utility model detection card.Wherein 1 is sample pad, and 2 is gold-marking binding pad, and 3 is NC film, and 4 is base plate, and 5 is absorbent paper, and 6 is nature controlling line for detection line 2,7 for detection line 1,8.
Fig. 2 is this utility model detection card top view, and 9 is well, and 10 is observation window.
Detailed description of the invention:
Below in conjunction with accompanying drawing, embodiment of the present utility model is elaborated.The present embodiment premised on technical solutions of the utility model under be carried out, give detailed embodiment and concrete operating process, but specific embodiment be only used for explaining this utility model rather than restriction this utility model.
2 colloidal gold strips respectively Myoglobin (Myo), cardic fatty acid binding protein (h-FABP) colloidal gold strip and creatine kinase isozyme (CK-MB), Troponin I (cTnI) colloidal gold strip in this utility model, article two, during test strips is placed in side by side and gets stuck, and the structure composition of two test strips is identical with manufacturing process.
Embodiment:
1. the preparation of gold colloidal
By 0.01% chlorauric acid solution heated and boiled, it is rapidly added the trisodium citrate 950uL of 2% afterwards；
The color of solution is become dark blue from light blue, continues heating and cherry-red occurs, continues heating 10min；Transparent cherry-red occurs, stops heating, continue to stir to room temperature.Thus it is prepared for the colloidal gold solution of 40nm.The quality of colloidal gold solution is prepared, it is ensured that the gold grain of preparation is in the same size, uniform, and particle diameter is at about 40nm with Electronic Speculum microscopy.
2. the preparation of Myoglobin (Myo), cardic fatty acid binding protein (h-FABP) colloidal gold strip
Take 1mL colloidal gold solution, be added thereto to appropriate 0.2M solution of potassium carbonate, concussion mixing, measure pH and pH value is adjusted to about 7.8；Then Myoglobin (Myo) the antibody 10uL adding 1mg/mL mixes, and vibrate 30min on agitator；Adding the 20%BSA of 10uL, mixing, vibrate 15min；10000rpm in centrifuge, centrifugal 10min, absorb supernatant gently；By the resuspended gold colloidal precipitation of 1mL cleaning mixture, inhale gently with pipettor and beat several times；10000rpm in centrifuge, centrifugal 10min, absorb supernatant gently；Wash again once, centrifugal, remove supernatant.By same method by cardic fatty acid binding protein (h-FABP) antibody labeling on colloid gold particle surface, then two kinds of gold colloidal immune complexs are mixed in the ratio of 1:1, again with multiple solution dilution, making the colloid gold immune complex after dilution at 530nm place OD(absorbance) value is 1.2-1.5；Point sample on non-woven fabrics, even spread, form glue gold pad, vacuum freeze-drying 3 hours, make gold-marking binding pad.
Respectively the antibody matched with Myoglobin (Myo) antibody of labelling, cardic fatty acid binding protein (h-FABP) antibody of 1mg/mL and 1.5mg/mL is drawn on the detection line (T line) of nitrocellulose filter, 1.0mg/mL sheep anti mouse polyclonal antibody is drawn on nature controlling line (C line).Then nitrocellulose filter is placed in baking oven 37 DEG C dry 2 days.
The gold-marking binding pad prepared and the nitrocellulose filter being coated being assembled by Fig. 1, the reagent paper batten that will paste above, carry out cutting with cutting cutter, bar width is set to 3.5mm.
3. creatine kinase isozyme (CK-MB), Troponin I (cTnI) colloidal gold strip
To prepare creatine kinase isozyme (CK-MB), Troponin I (cTnI) colloidal gold strip with Myoglobin (Myo), method that cardic fatty acid binding protein (h-FABP) colloidal gold strip is identical.Wherein the labelled amount of creatine kinase isozyme (CK-MB) antibody is 10ug/ml, and the labelled amount of Troponin I (cTnI) antibody is 20ug/ml；The package amount of creatine kinase isozyme (CK-MB) antibody is 1.5mg/ml, and the package amount of Troponin I (cTnI) antibody is 2mg/ml.
4. assemble
By the Myoglobin (Myo) prepared, cardic fatty acid binding protein (h-FABP) colloidal gold strip and creatine kinase isozyme (CK-MB), Troponin I (cTnI) colloidal gold strip, it is respectively charged into the correspondence position in getting stuck, the detection card that will assemble, loads aluminium foil bag and puts into desiccant sealing preservation simultaneously.
5. the use of test strips and result interpretation
Before detection, first sample serum/plasma and detection are placed under room temperature and balance 1 hour.
Then detection card keep flat, sample is added dropwise to detection card well in, add 3-4 drip sample, due to capillary effect, liquid is chromatography upwards, with the antibody being fixed on T line, C line and two anti-bindings and be trapped, colour developing.After 20min, judge yin and yang attribute result according to the colour developing situation of T, C line or coordinate readout instrument Accurate Determining result.When using readout instrument measurement result, after being set by the relevant parameter of instrument, test strips being put into reading position and is measured, instrument would indicate that sample concentration measurement result.
Above this utility model is done detailed description, its objective is man skilled in the art scholar can more fully be understood content of the present utility model and be carried out, not the restriction to embodiment of the present utility model.
Claims (5)
1. the test kit of a quantitative joint-detection myocardial infarction, it includes detection card and readout instrument, wherein detection card is got stuck by plastics and is placed side by side two colloidal gold strips therein and forms, plastics front of getting stuck is provided with two wells and two observation windows, article two, colloidal gold strip wherein one be Myoglobin (Myo) and cardic fatty acid binding protein (h-FABP) test strips, other one is creatine kinase isozyme (CK-MB) and Troponin I (cTnI) test strips, article 2, test strips is by sample pad, gold-marking binding pad, NC film and absorbent filter connected in order pasting successively forms on base plate.
2. the test kit of a kind of quantitative joint-detection myocardial infarction according to claim 1, it is characterised in that the solid phase colloid gold particle of Myoglobin (Myo) antibody and cardic fatty acid binding protein (h-FABP) antibody labeling on the gold-marking binding pad of described Myoglobin (Myo) and cardic fatty acid binding protein (h-FABP) test strips.
3. the test kit of a kind of quantitative joint-detection myocardial infarction according to claim 1, it is characterized in that on the nitrocellulose filter of Myoglobin (Myo) and cardic fatty acid binding protein (h-FABP) test strips, the coated antibody concentration matched with Myoglobin (Myo) antibody is 1.0mg/mL, the antibody concentration matched with cardic fatty acid binding protein (h-FABP) antibody is 1.5mg/mL.
4. the test kit of a kind of quantitative joint-detection myocardial infarction according to claim 1, it is characterised in that the solid phase colloid gold particle of creatine kinase isozyme (CK-MB) antibody and Troponin I (cTnI) antibody labeling on the gold-marking binding pad of creatine kinase isozyme (CK-MB) and Troponin I (cTnI) test strips.
5. the test kit of a kind of quantitative joint-detection myocardial infarction according to claim 1, it is characterized in that on the nitrocellulose filter of creatine kinase isozyme (CK-MB) and Troponin I (cTnI) test strips, the coated antibody concentration matched with creatine kinase isozyme (CK-MB) antibody is 1.5mg/mL, the antibody concentration matched with Troponin I (cTnI) antibody is 2.0mg/mL.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521060175.8U CN205374466U (en) | 2015-12-18 | 2015-12-18 | Ration jointly detects kit of miocardial infarction |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521060175.8U CN205374466U (en) | 2015-12-18 | 2015-12-18 | Ration jointly detects kit of miocardial infarction |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205374466U true CN205374466U (en) | 2016-07-06 |
Family
ID=56258399
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201521060175.8U Active CN205374466U (en) | 2015-12-18 | 2015-12-18 | Ration jointly detects kit of miocardial infarction |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205374466U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107870238A (en) * | 2016-09-28 | 2018-04-03 | 上海仪电分析仪器有限公司 | Troponin I in a kind of quantitative measurment human serum（cTnI）Method |
| CN108593919A (en) * | 2018-03-13 | 2018-09-28 | 深圳市第二人民医院 | A kind of colloidal gold immune chromatography test and its preparation method and application |
| CN109870327A (en) * | 2019-03-28 | 2019-06-11 | 李亚峰 | Glass microballoon quantitative sampling device |
| CN111208062A (en) * | 2020-02-28 | 2020-05-29 | 中国人民解放军陆军军医大学第二附属医院 | Colloidal gold type heart-shaped fatty acid and myoglobin detection device |
-
2015
- 2015-12-18 CN CN201521060175.8U patent/CN205374466U/en active Active
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107870238A (en) * | 2016-09-28 | 2018-04-03 | 上海仪电分析仪器有限公司 | Troponin I in a kind of quantitative measurment human serum（cTnI）Method |
| CN107870238B (en) * | 2016-09-28 | 2023-12-26 | 上海仪电分析仪器有限公司 | Method for quantitatively measuring troponin I (cTnI) in human serum |
| CN108593919A (en) * | 2018-03-13 | 2018-09-28 | 深圳市第二人民医院 | A kind of colloidal gold immune chromatography test and its preparation method and application |
| CN109870327A (en) * | 2019-03-28 | 2019-06-11 | 李亚峰 | Glass microballoon quantitative sampling device |
| CN109870327B (en) * | 2019-03-28 | 2024-01-05 | 李亚峰 | Glass bead quantitative sampling device |
| CN111208062A (en) * | 2020-02-28 | 2020-05-29 | 中国人民解放军陆军军医大学第二附属医院 | Colloidal gold type heart-shaped fatty acid and myoglobin detection device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108254562B (en) | Detect time-resolved fluoroimmunoassay chromatograph test strip, the kit and preparation method thereof of MYO | |
| CN108254550B (en) | Detect time-resolved fluoroimmunoassay chromatograph test strip, the kit and preparation method thereof of CK-MB | |
| CN108254563B (en) | Detect time-resolved fluoroimmunoassay chromatograph test strip, the kit and preparation method thereof of cTnI | |
| CN205374466U (en) | Ration jointly detects kit of miocardial infarction | |
| CN201087837Y (en) | Human myohemoglobin/creatine kinase isoenzyme/myocardium calcium protein I diagnosis test paper | |
| JP5012507B2 (en) | Analysis apparatus and analysis method | |
| CA1137410A (en) | Double tagged immunoassay | |
| CN104714008B (en) | A kind of immuno-chromatographic test paper strip and preparation method thereof and detection method | |
| CN108398562A (en) | Cystatin C fluorescent micro-ball immune chromatography quantitative testing test paper item and test card | |
| CN105891508A (en) | TRF (time-resolved fluorescence) immunochromatography reagent for rapidly and quantitatively detecting H-FABP (heart fatty acid-binding protein) and preparation method | |
| CN106153927A (en) | A kind of fast quantification detects time-resolved fluoroimmunoassay chromatography reagent and the preparation method of cTnI, CKMB, Myo simultaneously | |
| CN106248958A (en) | The fluorescence immune chromatography reagent of a kind of detection by quantitative cTnI and preparation method | |
| CN104345149A (en) | Immunochromatography test strip for detecting glycosylated hemoglubin and preparation method thereof | |
| JP4562854B2 (en) | Chromatographic measurement method | |
| WO2014067033A1 (en) | Method and kit for detection of haemoglobin in cervix uteri exudate | |
| CN108593919A (en) | A kind of colloidal gold immune chromatography test and its preparation method and application | |
| CN108398563A (en) | A kind of double labelling magnetic bead time-resolved fluoroimmunoassay quantitatively detects cTnI and H-FABP kits | |
| CN113156105A (en) | A type botulinum toxin rapid quantitative detection card | |
| CN201087838Y (en) | Myocardium calcium protein I color particle diagnosis test paper | |
| CN205484371U (en) | Tumour mark detect reagent box | |
| CN102707056A (en) | Immunofluorescence test strip component for quickly and quantitatively detecting myocardial creatine kinase isozyme, detection card component comprising same and preparation method | |
| CN101738476B (en) | Rapid diagnosis kit for pre-S1 antigens of hepatitis B viruses and method for preparing same | |
| CN108918865A (en) | Fluorescence immune chromatography test paper bar and reagent card | |
| CN109655625A (en) | A kind of colloidal gold strip and its preparation method and application detecting glycosylated hemoglobin | |
| CN209264735U (en) | A kind of high pass amount detecting device for two-step method immunochromatography |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |