CN203224492U - Test strip and kit for in-vitro joint inspection and diagnosis - Google Patents
Test strip and kit for in-vitro joint inspection and diagnosis Download PDFInfo
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- CN203224492U CN203224492U CN 201320105257 CN201320105257U CN203224492U CN 203224492 U CN203224492 U CN 203224492U CN 201320105257 CN201320105257 CN 201320105257 CN 201320105257 U CN201320105257 U CN 201320105257U CN 203224492 U CN203224492 U CN 203224492U
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- joint inspection
- external joint
- sample pad
- test paper
- test strip
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Abstract
The utility model discloses a test strip for in-vitro joint inspection and diagnosis. The test strip comprises a plurality of immunochromatographic assay test strips, wherein the plurality of immunochromatographic assay test strips share a sample pad; the sample pad forms a radiation shape. The utility model also discloses a kit for in-vitro joint inspection and diagnosis. The kit comprises a shell, wherein the test strip for in-vitro joint inspection and diagnosis is fixedly arranged inside the shell; a sampling hole is formed in the area corresponding to the sample pad on the shell; the shell is also provided with an observation window for reading detection results of the immunochromatographic assay test strips. The test strip and the kit disclosed by the utility model can achieve simultaneous detection of a plurality of items; a user does not need a lot of repeated work. Thus, the labor intensity can be reduced; in addition, the dosage of the sample also can be reduced.
Description
Technical field
The utility model relates to immunochromatography and detects articles for use, particularly relates to a kind of external joint inspection diagnosis test paper and kit.
Background technology
In recent decades, worldwide irritated crowd is increasing.Irritated this concept typically refers to I type hypersensitivity (type), causes symptom in contact allergy after former 30 minutes usually.The anaphylactogen that causes the hypersensitivity of I type is the protein from physical environment mostly, as plant pollen, animal skin, food, dirt mite and insect venom etc.The distinguishing feature of I type hypersensitivity is that anaphylactogen combines (sIgE) with specific immune globulin E, therefore detects the important means that sIgE becomes present anaphylodiagnosis.
Immunochromatographic method (immunochromatography) is a kind of quick diagnosis technology of abroad rising in recent years, its principle is a certain district band that special antibody is fixed in nitrocellulose filter earlier, after this dry cellulose nitrate one end immerses sample (urine or serum etc.), because capillarity, sample will move forward along this film, when moving to when antibody regional fixedly arranged, corresponding antigen namely is combined with this antibody generation specificity in the sample, if can make this zone show certain color with immune colloid gold or immuno-enzymatic dyeing, thereby realize specific immunodiagnosis.
In recent years, providing reliable, instant testing result is one of developing direction of laboratory medicine.This demand is impelled various can constantly the release at medical science detection (POCT) product that the patient carries out at one's side.The golden label immunoassay technology that early 1990s grows up has obtained using widely clinically, and the test item that relates to comprises infectious diseases antigen, antibody, tumor markers, the detection of hormone and medicine.
Existing anaphylactogen diagnostic method mainly is the detection at single anaphylactogen, as dirt mite, pollen, cat hair etc.Existing latex beads allergenic specific IgE testing product also can only detect single anaphylactogen.But only do the explanation patient's body situation that a detection can not be complete, will do multinomial detection in clinical, therefore the work that not only needs to consume a large amount of serum but also need to do a lot of repetitions demand developing a kind of product that can detect multinomial anaphylactogen simultaneously urgently.
The utility model content
The purpose of this utility model provides a kind of external joint inspection diagnosis test paper and paper box that can carry out multinomial detection simultaneously, makes the user need not much to repeat work, reduces labour intensity.
For achieving the above object, the utility model adopts following technical scheme:
A kind of external joint inspection diagnosis test paper comprises many immunochromatographydetecting detecting test strips, and described many immunochromatographydetecting detecting test strips share a sample pad.
Further, described sample pad radially, described immunochromatographydetecting detecting test strip also comprises by mark zone, nitrocellulose filter detection zone and absorbent filter district and is connected the workspace of forming successively, and each radiation band of described sample pad is connected with each bar workspace respectively.
Further, described sample pad is almost fan, and protrudes a plurality of bands in a side direction external radiation of fan-shaped arc.
Further, described sample pad one side also is provided with the pad ear of being convenient to take.
Further, the nitrocellulose filter detection zone of described many immunochromatographydetecting detecting test strips is coated with different anaphylactogens respectively, and the mark zone is coated with the anti-IgE antibodies of trace particle mark respectively.
Further, described trace particle is latex beads.
Further, described immunochromatographydetecting detecting test strip is 5.
The utility model also provides a kind of external joint inspection diagnostic kit, comprise housing, be set with aforesaid external joint inspection diagnosis test paper in the described housing, the zone of counter sample pad has well on the described housing, and also is provided with the watch window be used to the testing result that reads described immunochromatographydetecting detecting test strip on the described housing.
Further, described housing comprises loam cake and the lower cover of working in coordination, and described loam cake and/or lower cover are provided with groove or the rib for fixing described external joint inspection diagnosis test paper.
Because adopt technique scheme, the utility model has the following advantages at least:
(1) owing to adopt the version that shares sample pad, can enough realize multinomial order joint inspection, the user need not much to repeat work, has reduced user's labour intensity.
(2) owing to improved the shape of sample pad, make it radially, almost fan structure especially, multinomial visual inspection can reduce the consumption of sample when surveying.
(3) because the nitrocellulose filter detection zone of many immuno-chromatographic test paper strips is coated with different anaphylactogens respectively, can fast detecting go out the specific IgE antibody that whether contains in serum, blood plasma or the whole blood at certain anaphylactogen.
(4) owing to adopt new kit, be used for loading above-mentioned external joint inspection diagnosis test paper, a step simple to operate finishes, visual result as seen, and preserve convenient.
Description of drawings
Above-mentioned only is the general introduction of technical solutions of the utility model, and for can clearer understanding technological means of the present utility model, the utility model is described in further detail below in conjunction with accompanying drawing and embodiment.
Fig. 1 is the sample pad structural representation.
Fig. 2 is the immunochromatographydetecting detecting test strip side view.
Fig. 3 is a kind of external joint inspection diagnostic kit structural representation of the present utility model.
Embodiment
Immunochromatographydetecting detecting test strip of the prior art as shown in Figure 1, by base plate 1 and be connected sample pad 2, the trace particle bond pad 3(mark zone be arranged on the base plate 1 successively), nitrocellulose filter detection zone 4 and absorbent filter 5 form.The test strips of this kind structure only can be carried out individual event and be detected, and in the time of will doing multinomial detection in clinical, need do the work of a lot of repetitions.
On the basis of said structure, the utility model adopts many immunochromatographydetecting detecting test strips to share the version of a sample pad, can address the above problem.
In the above-described embodiments, sample pad can be radially, and each radiation band of sample pad is connected with the workspace of each bar immunochromatographydetecting detecting test strip (be connected successively be made up of mark zone, nitrocellulose filter detection zone and absorbent filter district) respectively, can reduce the consumption of sample.
As shown in Figure 2, sample pad is preferably almost fan, and protrudes a plurality of bands 21 in a side direction external radiation of fan-shaped arc, is 5 among the figure.Sample pad one side also is provided with the pad ear of being convenient to take 22.
Following mask body is that example is elaborated with a kind of immunochromatographydetecting detecting test strip that can detect multiple anaphylactogen simultaneously.
On the basis of said structure, the nitrocellulose filter detection zone of many immunochromatographydetecting detecting test strips is coated with different anaphylactogens respectively and (makes up as mite, cat fur bits, the dog skin bits, Alternaria, the branch spore is mould etc.), and the mark zone is coated with the anti-IgE antibodies of trace particle mark respectively, is connected with anti human IgE antibody as selecting the blue polystyrene microsphere of diameter 0.3-0.5um in the present embodiment.
Wherein, microballoon and the method for attachment of anti human IgE antibody are as follows:
1. anti human IgE antibody is dissolved in the deionized water, makes its concentration reach 1mM.
2. with microballoon storage liquid concuss 20s, take out 5.0 * 10
6Microballoon in the Eppendorf centrifuge tube.
3.8000g the centrifugal 1-2min of above rotating speed.
4. add 50ul100mM MES(PH:4.5 after removing supernatant), concuss 20s.、
5. prepare 1:10 dilution antibody, and get 2ul and place the centrifuge tube that microballoon is housed.
6. add the EDC of the freshly prepared 10mg/ml of 2.5ul, concuss mixes it.
7. 30min is hatched in the room temperature darkroom.
8. add 1.0ml0.02%Tween-20 to coupling microballoon.
9.8000g the centrifugal 1-2min of above rotating speed.
10. remove supernatant, the microballoon of coupling is dissolved in 1.0ml0.1%SDS, concuss.
11.8000g the centrifugal 1-2min of above rotating speed.
12. remove supernatant, make it be dissolved in the PBS(PH:8.0 of 100mM), concuss 20s.
13. placing 4 degree refrigerators then preserves.
As shown in Figure 3, a kind of external joint inspection diagnostic kit of the present utility model, comprise housing 6, be set with aforesaid external joint inspection diagnosis test paper in the housing 6, the zone of counter sample pad has well 7 on the housing 6, and also is provided with the watch window 8 be used to the testing result that reads described immunochromatographydetecting detecting test strip on the housing 6.The anaphylactogen name information (the mite combination, cat fur bits, the dog skin bits, Alternaria, the branch spore is mould etc.) can be labeled on the correspondence position of housing 6.
The utility model can fast detecting go out the specific IgE antibody that whether contains in serum, blood plasma or the whole blood at anaphylactogen, makes the user need not much to repeat work, reduces labour intensity.
The above; it only is preferred embodiment of the present utility model; be not that the utility model is done any pro forma restriction, those skilled in the art utilize the technology contents of above-mentioned announcement to make a little simple modification, equivalent variations or modification, all drop in the protection domain of the present utility model.
Claims (9)
1. an external joint inspection diagnosis test paper is characterized in that, comprises many immunochromatographydetecting detecting test strips, and described many immunochromatographydetecting detecting test strips share a sample pad.
2. a kind of external joint inspection diagnosis test paper according to claim 1, it is characterized in that, described sample pad radially, described immunochromatographydetecting detecting test strip also comprises by mark zone, nitrocellulose filter detection zone and absorbent filter district and is connected the workspace of forming successively, and each radiation band of described sample pad is connected with each bar workspace respectively.
3. a kind of external joint inspection diagnosis test paper according to claim 2 is characterized in that described sample pad is almost fan, and protrudes a plurality of bands in a side direction external radiation of fan-shaped arc.
4. a kind of external joint inspection diagnosis test paper according to claim 3 is characterized in that, described sample pad one side also is provided with the pad ear of being convenient to take.
5. according to claim 2,3 or 4 described a kind of external joint inspection diagnosis test papers, it is characterized in that, the nitrocellulose filter detection zone of described many immunochromatographydetecting detecting test strips is coated with different anaphylactogens respectively, and the mark zone is coated with the anti-IgE antibodies of trace particle mark respectively.
6. a kind of external joint inspection diagnosis test paper according to claim 5 is characterized in that described trace particle is latex beads.
7. a kind of external joint inspection diagnosis test paper according to claim 5 is characterized in that described immunochromatographydetecting detecting test strip is 5.
8. external joint inspection diagnostic kit, it is characterized in that, comprise housing, fixedly install each described external joint inspection diagnosis test paper just like claim 1-7 in the described housing, the zone of counter sample pad has well on the described housing, and also is provided with the watch window be used to the testing result that reads described immunochromatographydetecting detecting test strip on the described housing.
9. a kind of external joint inspection diagnostic kit according to claim 8 is characterized in that described housing comprises loam cake and the lower cover of working in coordination, and described loam cake and/or lower cover are provided with groove or the rib for fixing described external joint inspection diagnosis test paper.
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CN 201320105257 CN203224492U (en) | 2013-03-08 | 2013-03-08 | Test strip and kit for in-vitro joint inspection and diagnosis |
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
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CN105675603A (en) * | 2015-05-16 | 2016-06-15 | 贾晓轻 | Clinical symptom screening kit |
CN106018768A (en) * | 2016-05-31 | 2016-10-12 | 四川金域医学检验中心有限公司 | Multi-project biochemical examination and analysis integrated kit |
CN106526178A (en) * | 2016-12-08 | 2017-03-22 | 刘宏川 | Continuous quantitative readable detection device adopting immunochromatography |
CN107247139A (en) * | 2017-08-06 | 2017-10-13 | 潘荣兰 | It is a kind of can quick detection contain the chip of transgene component crop |
WO2019109365A1 (en) * | 2017-12-06 | 2019-06-13 | 深圳市易瑞生物技术股份有限公司 | Immunochromatography detection device for high-flux agricultural veterinary drug residues |
CN116359498A (en) * | 2023-02-23 | 2023-06-30 | 兰州兽研生物科技有限公司 | Immune chromatography test paper card for joint inspection of foot-and-mouth disease, african swine fever, swine fever and porcine reproductive and respiratory syndrome virus antibodies |
-
2013
- 2013-03-08 CN CN 201320105257 patent/CN203224492U/en not_active Expired - Fee Related
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105675603A (en) * | 2015-05-16 | 2016-06-15 | 贾晓轻 | Clinical symptom screening kit |
CN105675603B (en) * | 2015-05-16 | 2018-07-06 | 乔爱军 | A kind of clinical symptoms kit for screening |
CN106018768A (en) * | 2016-05-31 | 2016-10-12 | 四川金域医学检验中心有限公司 | Multi-project biochemical examination and analysis integrated kit |
CN106526178A (en) * | 2016-12-08 | 2017-03-22 | 刘宏川 | Continuous quantitative readable detection device adopting immunochromatography |
CN107247139A (en) * | 2017-08-06 | 2017-10-13 | 潘荣兰 | It is a kind of can quick detection contain the chip of transgene component crop |
WO2019109365A1 (en) * | 2017-12-06 | 2019-06-13 | 深圳市易瑞生物技术股份有限公司 | Immunochromatography detection device for high-flux agricultural veterinary drug residues |
CN116359498A (en) * | 2023-02-23 | 2023-06-30 | 兰州兽研生物科技有限公司 | Immune chromatography test paper card for joint inspection of foot-and-mouth disease, african swine fever, swine fever and porcine reproductive and respiratory syndrome virus antibodies |
CN116359498B (en) * | 2023-02-23 | 2024-06-11 | 兰州兽研生物科技有限公司 | Immune chromatography test paper card for joint inspection of foot-and-mouth disease, african swine fever, swine fever and porcine reproductive and respiratory syndrome virus antibodies |
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C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131002 Termination date: 20140308 |