CN106596993A - Press-type ELISA (enzyme-linked immunosorbent assay) sample loading device - Google Patents
Press-type ELISA (enzyme-linked immunosorbent assay) sample loading device Download PDFInfo
- Publication number
- CN106596993A CN106596993A CN201710056570.6A CN201710056570A CN106596993A CN 106596993 A CN106596993 A CN 106596993A CN 201710056570 A CN201710056570 A CN 201710056570A CN 106596993 A CN106596993 A CN 106596993A
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- CN
- China
- Prior art keywords
- sample loading
- loading
- pipe
- type elisa
- sleeve pipe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1065—Multiple transfer devices
- G01N35/1067—Multiple transfer devices for transfer to or from containers having different spacing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1065—Multiple transfer devices
- G01N35/1074—Multiple transfer devices arranged in a two-dimensional array
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N2035/1027—General features of the devices
- G01N2035/1034—Transferring microquantities of liquid
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a press-type ELISA (enzyme-linked immunosorbent assay) sample loading device. The press-type ELISA sample loading device comprises a plurality of sample loading tubes and an external holder. The external holder is provided with a plurality of hollowed-out holes in matrix arrangement and in correspondence with a 96-well plate. The front end of every sample loading tube is embedded into the corresponding hollowed-out hole. Every sample loading tube is provided with a plunger, and a user presses the plungers simultaneously through a pressing plate, so that adding samples into multiple holes simultaneous is achieved, sample loading time is shortened, consistency in reaction time of all the holes is guaranteed maximally, and experimental efficiency and accuracy are improved greatly. The press-type ELISA sample loading device has the advantages of simplicity in manufacture, low cost, convenience and durability and is applicable to scientific research application and commercial popularization.
Description
Technical field
The present invention relates to the device of ELISA is applied to during a kind of scientific research, more particularly to a kind of push type ELISA loadings dress
Put.
Background technology
Enzyme-linked immunosorbent assay (ELISA), is one of common molecular biology experiment, is widely used in school and scientific research
Mechanism, its principle is, in surface of solid phase carriers, to make the antigen-antibody reaction that enzyme is marked solid by known antigen or antibody absorption
Phase surface is carried out, and the technology can be used to detect macromolecular antigen and specific antibody etc., easy with quick, sensitive, easy, carrier
The advantages of standardization.Conventional ELISA kit mostly is 96 orifice plates, when large sample is used for the experiment to be detected, from loading
First hole takes longer, error increase to last hole, cannot more ensure that the reaction time in every hole is consistent, greatly interference
Experimental result；In addition to content added by the every hole of standard items and sample is different, a remaining anti-working solution, enzyme labelled antibody work
Liquid, substrate working solution and last terminate liquid, are the content liquid such as every hole equivalent.Therefore, this device is designed, realizes same content
Deng quantity of fluid while add, greatly shorten loading time, be more beneficial for reducing the experimental error that causes of manual operation, it is right
The efficiency and accuracy for improving experiment plays strong impetus.
The content of the invention
The purpose of the present invention is the problems such as having unstability, longer consuming time, waste of resource for existing operation, is carried
For a kind of push type ELISA sampling devices.
The purpose of the present invention is achieved through the following technical solutions：A kind of push type ELISA sampling devices, the device bag
Include multiple loading pipes and outrigger；Outrigger is provided with multiple and loading pipe front end size identical hollow hole, and hollow hole is arranged in matrix,
It is corresponding with 96 orifice plates；The front end of loading pipe is embedded in hollow hole；The loading pipe includes external sleeve pipe, piston-in-sleeve, set
Head base, check valve sheet；Piston-in-sleeve one end is located in external sleeve pipe, is coordinated with external sleeve interference, and the other end is extended to
Outside external sleeve pipe；Piston-in-sleeve extension in all loading pipes is highly consistent；External casing part is provided with check valve sheet, single
It is the accommodation space for treating loading liquid for storage to valve block lower section.External its lower end is sealed by pullover base.
Further, also including pressing plate, pressing plate is placed in piston-in-sleeve top.
The invention has the beneficial effects as follows：Carry out method of operating when ELISA is tested for current scientific research to be integrated and weighed
New design, is assembled using current scientific and technological achievement, and efficiency and uniformity can be improved in process of scientific research, and is saved into
The waste of sheet and reduction resource, it is adaptable to which each universities and colleges and scientific research institution carry out experimental implementation, it is easy to promote.
Description of the drawings
Fig. 1 is the structural representation of loading pipe；
Fig. 2 is the loading schematic diagram of the present invention；
Fig. 3 is the structural representation of outrigger；
In figure, external sleeve pipe 1, piston-in-sleeve 2, pullover base 4, check valve sheet 5, loading liquid 6, pressing plate 8,96 are treated
Orifice plate 9, outrigger 10, hollow hole 11.
Specific embodiment
As Figure 1-3, a kind of push type ELISA sampling devices, the device is made up of multiple loading pipes and outrigger 10；Institute
Loading pipe is stated including external sleeve pipe 1, piston-in-sleeve 2, pullover base 4, check valve sheet 5；Outrigger 10 is provided with multiple and loading pipe
Front end size identical hollow hole 11, hollow hole 11 is arranged in matrix, corresponding with 96 orifice plates etc.；The front end of loading pipe is embedded in engraves
In emptying aperture 11.The one end of piston-in-sleeve 2 is located in external sleeve pipe 1, and with the interference fit of external sleeve pipe 1, the other end extends to external
Outside sleeve pipe 1；The extension of piston-in-sleeve 2 highly consistent (position in external sleeve pipe 1 is identical) in all loading pipes.External set
The bottom of pipe 1 is provided with check valve sheet 5, and the lower section of check valve sheet 5 is the accommodation space for treating loading liquid 6 for storage.External sleeve pipe 1
Lower end is sealed by pullover base 4.
Said apparatus can pass through conventional plate and all piston-in-sleeves 2 are pressed, it is also possible to arrange supporting pressing
Pressing plate 8.
The device course of work is following (by taking 96 orifice plates as an example)：
Outrigger 10 is placed in into the top of 96 orifice plate 9, external sleeve pipe 1 is embedded in hollow hole 11 and is fixed, then put pressing plate 8
On external sleeve pipe 1, the pullover base 4 of often pipe is removed, then alignment loading hole firmly presses pressing plate 8, piston-in-sleeve 2
Push, open pipe internal check valve piece 5, treat that loading liquid injects in loading hole simultaneously.
Situation in prevent the inwhole hand-holes of individual fluid occurs, and can also manually press do not use up liquid
Piston-in-sleeve 2, it is ensured that in stand-by liquid whole hand-hole.
Claims (2)
1. a kind of push type ELISA sampling devices, it is characterised in that the device includes multiple loading pipes and outrigger (10)；Outrigger
(10) multiple hollow holes (11) are provided with, hollow hole (11) is arranged in matrix, corresponding with 96 orifice plates；The front end of loading pipe is embedded in engraves
In emptying aperture (11)；The loading pipe includes external sleeve pipe (1), piston-in-sleeve (2), pullover base (4), check valve sheet (5)；Set
Pipe inner carrier (2) one end is located in external sleeve pipe (1), and with external sleeve pipe (1) interference fit, the other end extends to external sleeve pipe
(1) outward；Piston-in-sleeve (2) extension in all loading pipes is highly consistent；External sleeve pipe (1) bottom is provided with check valve sheet
(5), check valve sheet (5) lower section is the accommodation space for treating loading liquid (6) for storage.External sleeve pipe (1) lower end passes through pullover
Base (4) is sealed.
2. push type ELISA sampling devices according to claim 1, it is characterised in that also including pressing plate (8), pressing
Plate (8) is placed in piston-in-sleeve (2) top.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710056570.6A CN106596993A (en) | 2017-01-25 | 2017-01-25 | Press-type ELISA (enzyme-linked immunosorbent assay) sample loading device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710056570.6A CN106596993A (en) | 2017-01-25 | 2017-01-25 | Press-type ELISA (enzyme-linked immunosorbent assay) sample loading device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106596993A true CN106596993A (en) | 2017-04-26 |
Family
ID=58585288
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710056570.6A Pending CN106596993A (en) | 2017-01-25 | 2017-01-25 | Press-type ELISA (enzyme-linked immunosorbent assay) sample loading device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106596993A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109856392A (en) * | 2019-02-22 | 2019-06-07 | 武汉巴菲尔生物技术服务有限公司 | The device for fast detecting and method of interaction of biomacromolecules |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3568735A (en) * | 1968-06-26 | 1971-03-09 | Cooke Eng Co | Laboratory microtitration dispensing apparatus |
| US6374683B1 (en) * | 1999-01-29 | 2002-04-23 | Genomic Instrumentation Services, Inc. | Pipetter |
| CN201543431U (en) * | 2009-08-06 | 2010-08-11 | 上海精睿医疗器械有限公司 | Multi-channel micropipettor |
| DE102012207898A1 (en) * | 2011-05-11 | 2012-11-15 | Umwelt-Geräte-Technik GmbH | Piston stroke pipette for determination device for determining particle size distribution in soil samples to classify agricultural soil, has non-return valve arranged in flow path between cylinder and aperture of suction pipe |
| CN203551564U (en) * | 2013-08-30 | 2014-04-16 | 上海市儿童医院 | Manual sample feeding auxiliary device of ELISA (Enzyme Linked Immunosorbent Assay) micro-porous plate |
-
2017
- 2017-01-25 CN CN201710056570.6A patent/CN106596993A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3568735A (en) * | 1968-06-26 | 1971-03-09 | Cooke Eng Co | Laboratory microtitration dispensing apparatus |
| US6374683B1 (en) * | 1999-01-29 | 2002-04-23 | Genomic Instrumentation Services, Inc. | Pipetter |
| CN201543431U (en) * | 2009-08-06 | 2010-08-11 | 上海精睿医疗器械有限公司 | Multi-channel micropipettor |
| DE102012207898A1 (en) * | 2011-05-11 | 2012-11-15 | Umwelt-Geräte-Technik GmbH | Piston stroke pipette for determination device for determining particle size distribution in soil samples to classify agricultural soil, has non-return valve arranged in flow path between cylinder and aperture of suction pipe |
| CN203551564U (en) * | 2013-08-30 | 2014-04-16 | 上海市儿童医院 | Manual sample feeding auxiliary device of ELISA (Enzyme Linked Immunosorbent Assay) micro-porous plate |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109856392A (en) * | 2019-02-22 | 2019-06-07 | 武汉巴菲尔生物技术服务有限公司 | The device for fast detecting and method of interaction of biomacromolecules |
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Application publication date: 20170426 |