CN109856392A - The device for fast detecting and method of interaction of biomacromolecules - Google Patents
The device for fast detecting and method of interaction of biomacromolecules Download PDFInfo
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- CN109856392A CN109856392A CN201910134366.0A CN201910134366A CN109856392A CN 109856392 A CN109856392 A CN 109856392A CN 201910134366 A CN201910134366 A CN 201910134366A CN 109856392 A CN109856392 A CN 109856392A
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Abstract
The present invention provides the device for fast detecting and method of a kind of interaction of biomacromolecules, including multiple detection pipes, multiple reaction tubes and outrigger；Outrigger is provided with multiple hollow holes, and hollow hole is arranged in matrix, corresponding with 96 orifice plates；The front end of detection pipe is embedded in the reaction tube in hollow hole；The detection pipe includes external casing, piston-in-sleeve, check valve sheet, sensing chamber；Piston-in-sleeve one end is located in external casing, cooperates with external sleeve interference, and the other end extends to outside external casing；Piston-in-sleeve extension height in all detection pipes is consistent；External casing part is equipped with check valve sheet, is the sensing chamber for storage reaction thing liquid body above check valve sheet, and it is detection pipe suction nozzle below check valve sheet, for drawing reactant liquid from the reaction tube that, which there is tool magnetic metal detection line in detection interior,.
Description
Technical field
The present invention relates to field of biotechnology, more particularly, to a kind of quick detection of interaction of biomacromolecules
Device and method.
Background technique
Enzyme-linked immunosorbent assay (Enzyme-Linked Immunosorbent Assays, ELISA) was from quilt in 1971
Since Engvall and Perlman is reported, it has been widely used in immunology and biochemical field.Not with method
Disconnected improvement, the continuous renewal of microplate reader, in the context of detection of antigen and antibody, ELISA is still in current laboratory testing analysis
One of most common technology.The basic principle of ELISA method is antigen with after antibody molecule covalent bond, does not influence respective exempt from
Epidemiology activity.Developed the color by antigen coat, closing plus primary antibody plus ELIAS secondary antibody, substrate and etc., determine that antigen and antibody are anti-
The specificity answered determines the concentration of antibody or antigen by the depth of color reaction.Such chromogenic reaction can be detected by ELISA
Instrument (microplate reader) is quantitative determined, and ELISA method is made to become a kind of not only special but also sensitive detection method.However, ELISA is anti-
It should develop the color from antigen coat to substrate, need multiple steps, need even stay overnight in time within 4-6 hours.It is commercialized at present
ELISA detection kit, it is general using the ELISA Plate containing pre-coated antigen or antibody, but when detecting between on there is still a need for 2-
4 hours as long as.
Therefore, current detection technique needs solved the problems, such as quickly to detect reaction from the time.For this purpose, immune colloid gold layer
Analysis technology starts to come out.Chromatography detection technique comes across 1988 earliest, is that Unipath company utilizes nano-colloid gold particle
A kind of pregnant inspection chromatograph test strip of development and production can go out testing result using prefabricated test strips 3-5 minutes.Hereafter, it is immunized
Colloidal gold is widely used and develops in quick detection reagent.But colloidal gold technique needs different nano particles
The equipment such as colloidal gold production, metal spraying, point sample, and chromatograph to react often to have various reactions with ELISA and cause.It can go out in ELISA
Existing antigen and antibody response, does not occur but in colloid gold chromatographic test paper strip.
Summary of the invention
The present invention provides a kind of a kind of large biological molecule for overcoming the above problem or at least being partially solved the above problem
The device for fast detecting and method of interaction, suitable for ELISA principle detection all biomolecule, have it is efficient, simple and direct,
Quickly, conveniently, be applicable in the features such as wide.
First aspect according to an embodiment of the present invention provides a kind of quick detection dress of interaction of biomacromolecules
It sets, including multiple detection pipes, multiple reaction tubes and outrigger；Outrigger is provided with multiple hollow holes, and hollow hole is arranged in matrix, with 96 holes
Plate is corresponding；The front end of detection pipe is embedded in the reaction tube in hollow hole；The detection pipe includes external casing, living in casing
Plug, check valve sheet, sensing chamber；Piston-in-sleeve one end is located in external casing, cooperates with external sleeve interference, and the other end extends
To outside external casing；Piston-in-sleeve extension height in all detection pipes is consistent；External casing part is equipped with check valve sheet,
It is the sensing chamber for storage reaction thing liquid body above check valve sheet, there is the magnetic metal detection line of tool in detection interior, unidirectionally
It is detection pipe suction nozzle below valve block, for drawing reactant liquid from the reaction tube.
Preferably, further including pull ring, the pull ring is placed in above piston-in-sleeve.
Preferably, the reaction tube, detection pipe are plastics, ceramics or polyethylene inertia material.
Preferably, the multiple reaction tube is placed in parallel in hollow hole.
The second aspect according to an embodiment of the present invention provides a kind of quick detection side of interaction of biomacromolecules
Method, comprising:
The first large biological molecule is coupled on immunomagnetic beads, substance to be detected and at least one mark are then sequentially added
Second of large biological molecule of note；It completes that reaction tube is added in reactant liquid after mixing in test tube, or successively to reaction
Reactant liquid is added in pipe；
The first described large biological molecule is any one in protein, polysaccharide, polypeptide, nucleic acid or chemicals, institute
It states substance to be detected and second of large biological molecule is any in protein, polypeptide, polysaccharide, toxin, chemicals or nucleic acid
Second of large biological molecule of one kind, the label can be in conjunction with substance to be detected；Label for second of large biological molecule
It is any one in colloidal gold, fluorescent material, chemiluminescent material or upper forwarding luminescent material；
Piston-in-sleeve is pulled up, makes the reactant liquid flow detection room in reaction tube, and will by check valve sheet
It is indoor that reactant liquid is retained in detection；If the first large biological molecule being coupled on magnetic bead, substance to be detected, label
Second of large biological molecule is interacted, and reactant liquid can be adsorbed in detection line in detection interior；By glimmering
Photodetector, chemiluminescence detector or upper forwarding photodetector detect the color change of metal detection line.
The present invention proposes the device for fast detecting and method of a kind of interaction of biomacromolecules, is suitable for ELISA principle
Detection all biomolecule, have the characteristics that it is efficient, simple and direct, quick, conveniently, be applicable in it is wide.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is this hair
Bright some embodiments for those of ordinary skill in the art without creative efforts, can be with root
Other attached drawings are obtained according to these attached drawings.
Fig. 1 is the device for fast detecting schematic diagram according to the interaction of biomacromolecules of the embodiment of the present invention.
Specific embodiment
With reference to embodiment, the embodiment of the present invention is furthur described in detail.Following embodiment is used for
Illustrate the present invention, but is not intended to limit the scope of the invention.
A kind of device for fast detecting of interaction of biomacromolecules, as shown in Figure 1, including multiple detection pipes 1, multiple anti-
It should pipe 2 and outrigger；Outrigger is provided with multiple hollow holes, and hollow hole is arranged in matrix, corresponding with 96 orifice plates；The front end of detection pipe 1 is embedding
Enter in the reaction tube 2 in hollow hole；The detection pipe includes external casing 11, piston-in-sleeve 12, check valve sheet 14, detection
Room 13；12 one end of piston-in-sleeve is located in external casing 11, is interference fitted with external casing 11, and the other end extends to external set
Outside pipe 11；12 extension height of piston-in-sleeve in all detection pipes 1 is consistent；External 11 lower part of casing is equipped with check valve sheet
14, it is the sensing chamber 13 for storage reaction thing liquid body above check valve sheet 14, there is the magnetic metal inspection of tool in sensing chamber 13
Survey line 15 is detection pipe suction nozzle 16 below check valve sheet 14, for drawing reactant liquid from the reaction tube 2.
Preferably, further including pull ring 17, the pull ring 17 is placed in 12 top of piston-in-sleeve.
Preferably, the reaction tube 2, detection pipe 1 are plastics, ceramics or polyethylene inertia material.
Preferably, the multiple reaction tube 2 is placed in parallel in hollow hole.
The second aspect according to an embodiment of the present invention provides a kind of quick detection side of interaction of biomacromolecules
Method, comprising:
The first large biological molecule is coupled on immunomagnetic beads, substance to be detected and at least one mark are then sequentially added
Second of large biological molecule of note；It completes that reaction tube is added in reactant liquid after mixing in test tube, or successively to reaction
Reactant liquid is added in pipe；
The first described large biological molecule is any one in protein, polysaccharide, polypeptide, nucleic acid or chemicals, institute
It states substance to be detected and second of large biological molecule is any in protein, polypeptide, polysaccharide, toxin, chemicals or nucleic acid
Second of large biological molecule of one kind, the label can be in conjunction with substance to be detected；Label for second of large biological molecule
It is any one in colloidal gold, fluorescent material, chemiluminescent material or upper forwarding luminescent material；
Piston-in-sleeve is pulled up, makes the reactant liquid flow detection room in reaction tube, and will by check valve sheet
It is indoor that reactant liquid is retained in detection；If the first large biological molecule being coupled on magnetic bead, substance to be detected, label
Second of large biological molecule is interacted, and reactant liquid can be adsorbed in detection line in detection interior；By glimmering
Photodetector, chemiluminescence detector or upper forwarding photodetector detect the color change of metal detection line.
The present invention proposes the device for fast detecting and method of a kind of interaction of biomacromolecules, is suitable for ELISA principle
Detection all biomolecule, have the characteristics that it is efficient, simple and direct, quick, conveniently, be applicable in it is wide.
Finally, it should be noted that the above various embodiments is only to illustrate the technical solution of the embodiment of the present invention, rather than it is right
It is limited；Although the embodiment of the present invention is described in detail referring to foregoing embodiments, the ordinary skill of this field
Personnel are it is understood that it is still possible to modify the technical solutions described in the foregoing embodiments, or to part
Or all technical features are equivalently replaced；And these are modified or replaceed, it does not separate the essence of the corresponding technical solution
The range of each embodiment technical solution of the embodiment of the present invention.
Claims (5)
1. a kind of device for fast detecting of interaction of biomacromolecules, which is characterized in that including multiple detection pipes, multiple reactions
Pipe and outrigger；Outrigger is provided with multiple hollow holes, and hollow hole is arranged in matrix, corresponding with 96 orifice plates；The front end of detection pipe is embedded in
In reaction tube in hollow hole；The detection pipe includes external casing, piston-in-sleeve, check valve sheet, sensing chamber；It is living in casing
Plug one end is located in external casing, cooperates with external sleeve interference, and the other end extends to outside external casing；In all detection pipes
Piston-in-sleeve extension height is consistent；External casing part is equipped with check valve sheet, is for storage reaction above check valve sheet
There are the magnetic metal detection line of tool in the sensing chamber of thing liquid body, detection interior, are detection pipe suction nozzle below check valve sheet, for from
Reactant liquid is drawn in the reaction tube.
2. the device for fast detecting of interaction of biomacromolecules according to claim 1, which is characterized in that further include drawing
Ring, the pull ring are placed in above piston-in-sleeve.
3. the device for fast detecting of interaction of biomacromolecules according to claim 1, the reaction tube, detection pipe are equal
For plastics, ceramics or polyethylene inertia material.
4. the device for fast detecting of interaction of biomacromolecules according to claim 1, the multiple reaction tube is being engraved
It is placed in parallel in emptying aperture.
5. a kind of rapid detection method of interaction of biomacromolecules characterized by comprising
The first large biological molecule is coupled on immunomagnetic beads, substance to be detected and at least one label are then sequentially added
Second of large biological molecule；It completes that reaction tube is added in reactant liquid after mixing in test tube, or successively into reaction tube
Reactant liquid is added；
The first described large biological molecule is any one in protein, polysaccharide, polypeptide, nucleic acid or chemicals, it is described to
Detection substance and second of large biological molecule are any one in protein, polypeptide, polysaccharide, toxin, chemicals or nucleic acid,
Second of large biological molecule of the label can be in conjunction with substance to be detected；Label for second of large biological molecule is colloid
Gold, fluorescent material, chemiluminescent material or any one upper forwarded in luminescent material；
Piston-in-sleeve is pulled up, makes the reactant liquid flow detection room in reaction tube, and will react by check valve sheet
It is indoor that thing liquid body is retained in detection；If the first large biological molecule being coupled on magnetic bead, substance to be detected, label second
Kind large biological molecule is interacted, and reactant liquid can be adsorbed in detection line in detection interior；It is examined by fluorescence
Survey the color change that device, chemiluminescence detector or upper forwarding photodetector detect metal detection line.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201910134366.0A CN109856392A (en) | 2019-02-22 | 2019-02-22 | The device for fast detecting and method of interaction of biomacromolecules |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910134366.0A CN109856392A (en) | 2019-02-22 | 2019-02-22 | The device for fast detecting and method of interaction of biomacromolecules |
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| CN109856392A true CN109856392A (en) | 2019-06-07 |
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| CN201910134366.0A Pending CN109856392A (en) | 2019-02-22 | 2019-02-22 | The device for fast detecting and method of interaction of biomacromolecules |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6374683B1 (en) * | 1999-01-29 | 2002-04-23 | Genomic Instrumentation Services, Inc. | Pipetter |
| CN103439506A (en) * | 2013-08-12 | 2013-12-11 | 杭州戈登生物科技有限公司 | Rapid detection device and method for interaction of biomacromolecules |
| CN106596994A (en) * | 2017-01-25 | 2017-04-26 | 浙江中医药大学 | Pressing type ELISA sample adding apparatus capable of being recycled |
| CN106596993A (en) * | 2017-01-25 | 2017-04-26 | 浙江中医药大学 | Press-type ELISA (enzyme-linked immunosorbent assay) sample loading device |
| CN106596995A (en) * | 2017-01-25 | 2017-04-26 | 浙江中医药大学附属第三医院 | Reusable pneumatic ELISA sample loading apparatus |
| CN106596996A (en) * | 2017-01-25 | 2017-04-26 | 浙江中医药大学附属第三医院 | Pneumatic ELISA sample loading device |
| CN106932569A (en) * | 2015-12-29 | 2017-07-07 | 上海馨馨生物科技有限公司 | A kind of device for fast detecting of interaction of biomacromolecules |
-
2019
- 2019-02-22 CN CN201910134366.0A patent/CN109856392A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6374683B1 (en) * | 1999-01-29 | 2002-04-23 | Genomic Instrumentation Services, Inc. | Pipetter |
| CN103439506A (en) * | 2013-08-12 | 2013-12-11 | 杭州戈登生物科技有限公司 | Rapid detection device and method for interaction of biomacromolecules |
| CN106932569A (en) * | 2015-12-29 | 2017-07-07 | 上海馨馨生物科技有限公司 | A kind of device for fast detecting of interaction of biomacromolecules |
| CN106596994A (en) * | 2017-01-25 | 2017-04-26 | 浙江中医药大学 | Pressing type ELISA sample adding apparatus capable of being recycled |
| CN106596993A (en) * | 2017-01-25 | 2017-04-26 | 浙江中医药大学 | Press-type ELISA (enzyme-linked immunosorbent assay) sample loading device |
| CN106596995A (en) * | 2017-01-25 | 2017-04-26 | 浙江中医药大学附属第三医院 | Reusable pneumatic ELISA sample loading apparatus |
| CN106596996A (en) * | 2017-01-25 | 2017-04-26 | 浙江中医药大学附属第三医院 | Pneumatic ELISA sample loading device |
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Application publication date: 20190607 |