CN201269877Y - Multifunctional high flux immunity detection kit - Google Patents
Multifunctional high flux immunity detection kit Download PDFInfo
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- CN201269877Y CN201269877Y CNU2008200199528U CN200820019952U CN201269877Y CN 201269877 Y CN201269877 Y CN 201269877Y CN U2008200199528 U CNU2008200199528 U CN U2008200199528U CN 200820019952 U CN200820019952 U CN 200820019952U CN 201269877 Y CN201269877 Y CN 201269877Y
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Abstract
The utility model provides a multifunctional high-flux immunodetection reagent kit, which relates to the technical field of apparatus utilizing an immunolabelling technique for detection. The reagent kit comprises a bottom board 1, a point template 2, a point template 3, a cover plate 4, a cover plate 5, a silica gel plate 6, a nitrocellulose membrane 7, a water accepting layer 8, a microcontent spotting needle, a gold-labeled antibody, an enzyme labeled antibody, an enzyme substrate, confining liquid and washing liquid. Different combinations of the reagent kit can be applied to detection in many ways and application methods thereof are as follows: combination 1, immunogold filtration detection; combination 2, dot immunity enzyme-linked detection; combination 3, immunoblot detection; and combination 4, immunoblot gold filtration detection. The reagent kit is characterized in that one kit has multiple purposes and can detect the high flux of samples and the microcontent of the samples and reagents, with intelligible results, convenient use as well as economy and practicality. The point template 3 can be applied to the simultaneous detection of detected samples and checked samples by the same reagent. The immunoblot gold filtration detection has the detection speed faster than that of the conventional immunoblot detection by over 10 times.
Description
Technical field:
The utility model relates to the utensil technical field of utilizing immunolabelling technique to detect, and concrete is exactly multi-functional high flux immunity detection reagent.
Background technology:
At present, the clinical immunolabelling technique detection method of utilizing commonly used has: the immuno-enzymatic joint inspection is surveyed, immune-gold labeled detection, Western blotting etc.Different detection methods has own different utensil, and this brings certain to the user is economically burden, because of different appliance expensive; In addition every kind of utensil all has own different using method, thus use each utensil all need relearn and train.Do not reach the many usefulness of a utensil, simple, efficient.
Summary of the invention:
The present invention is with the clinical immunolabelling technique detection method of utilizing commonly used: the immuno-enzymatic joint inspection is surveyed, and immune-gold labeled detection, the detection of Western blotting are melted in a kind of appliance, promptly multi-functional high flux immunity detection reagent.Its technical scheme is: by base plate 1, and point template 2, point template 3, cover plate 4, cover plate 5 and silica gel plate 6, nitrocellulose filter 7, water accepting layer 8, the micro-sampling pen, golden labeling antibody, enzyme labelled antibody, the substrate of enzyme, confining liquid, cleaning fluid is formed, and it is characterized by: when this kit uses, it stacks order: base plate 1, water accepting layer 8, nitrocellulose filter 7, point template 2 or point template 3, cover plate 4 or cover plate 5, after the assembling, utensils such as available screw are fixing up and down with it, then reagent added in the hole on the cover plate.
Described cover plate 4: be 96 holes, up/down perforation, its arrangement mode and sign 96 well culture plates together, the floorage in hole is 25-55mm
2
Described point template 2: be 96 holes, up/down perforation, its arrangement mode is with 96 well culture plates, and the floorage in hole is 5-10mm
2, the position in each hole on it should be arranged in hole corresponding on the described cover plate 4 of claim 2.
Described point template 3: be 192 holes, up/down perforation, the floorage in hole is 3-4mm
2, per two Kong Weiyi group on it, the position in this group hole should be arranged in hole corresponding on the described cover plate 4 of claim 2.
Described cover plate 5: hole is a strip hole on it, up/down perforation, and the floorage in hole is 20-40mm
2
Described base plate 1, point template 2, point template 3, cover plate 4, cover plate 5: all leave the fixedly pin hole of nitrocellulose filter and water accepting layer on four jiaos of each plate, by this hole with pin fixedly nitrocellulose filter and water accepting layer, make it when changing cover plate and point template, the holding position is constant.
Described silica gel plate, totally 5, on its four jiaos, do not have the pin hole of fixing nitrocellulose filter or water accepting layer, its shape respectively with base plate 1, point template 2, point template 3, cover plate 4, cover plate 5 is identical, and respectively with the supporting use of above each plate, place it between nitrocellulose filter and each plate, prevent the effect of liquid seepage around the hole in each plate hole, if ne-leakage then can.
Described micro-sampling pen: be kapillary.
Described nitrocellulose filter, its membrane aperture are 0.2-0.8 μ m.
Described kit after its assembling, is used screw, or spring clip, or the spring clasp is fixed.
Described golden labeling antibody, enzyme labelled antibody, the substrate of enzyme, confining liquid, cleaning fluid all prepare by known method.
More than each assembly carry out different combinations and can carry out different detections.(1) combination one is used for immune golden diafiltration and detects, and combination comprises: base plate 1, point template 2 or point template 3, cover plate 4, nitrocellulose filter 7, water accepting layer 8, micro-sampling pen, golden labeling antibody.(2) combination two is used for the joint inspection of spot immune enzyme and surveys, and combination comprises: base plate 1, point template 2 or point template 3, cover plate 4, nitrocellulose filter 7, micro-sampling pen, enzyme labelled antibody, the substrate of enzyme.(3) combination three, Western blotting detects: base plate 1, cover plate 4, cellulose nitrate 7, enzyme labelled antibody, the substrate of enzyme.(4) combination four, the diafiltration of Western blotting gold detects, and combination comprises: base plate 1, cover plate 4, nitrocellulose filter 7, water accepting layer 8, golden labeling antibody.Silica gel plate, confining liquid, cleaning fluid are applicable to above various combination.
The advantage of this utility model is: the many usefulness of (1) one box, and can be used for immune golden diafiltration and detect, the joint inspection of spot immune enzyme is surveyed, and Western blotting detects and the diafiltration of Western blotting gold detects, and is easy, economical and practical; (2) application of point template and its matching used micro-sampling pen is that point sample reaches traceization.Point template 2 can be used for the detection of sample, and point template can 3 be used for same reagent test sample and control sample being detected simultaneously, and visual result.(3) cover plate 4,96 micropores design makes and detects sample high flux, detectable traceization; (4) combination four, the diafiltration of Western blotting gold detects, but goes out the result in the 10min, compares with traditional Western blotting 3h detection time, is significantly improved.
Description of drawings:
Further specify in conjunction with the accompanying drawings:
Fig. 1 is combination one assembling stereogram; Fig. 2 is combination two assembling stereograms; Fig. 3 is combination three assembling stereograms; Fig. 4 is combination four assembling stereograms.
Embodiment:
(1) combination one is used for immune golden diafiltration and detects, the 1st step: fix each plate by assembling from the bottom to top, base plate 1+ water accepting layer 8+ nitrocellulose filter 7+ point template 2 or point template 3, with the micro-sampling pen by the hole on the point template with sample spot on nitrocellulose filter.Dry sample.The 2nd goes on foot: point template is taken down changed cover plate 4, sample spot is arranged in the hole of cover plate 4 correspondences just, fixes each plate, then, in the hole of cover plate 4, by successively, add confining liquid and infiltrate, add golden labeling antibody and infiltrate, add cleaning fluid and infiltrate, the 3rd step: observations: spottiness is positive, otherwise negative.
(2) combination two, be used for the joint inspection of spot immune enzyme and survey, the 1st step: fix each plate, base plate 1+ nitrocellulose filter 7+ point template 2 or point template 3 by assembling from the bottom to top, with the micro-sampling pen by the hole on the point template with sample spot on nitrocellulose filter, dry sample.The 2nd goes on foot: point template is taken down changed cover plate 4, sample spot is arranged in hole corresponding on the cover plate 4 just, fixes each plate, then, in the hole of cover plate 4, by successively, adds confining liquid, enzyme labelled antibody, cleaning fluid, the substrate of enzyme, colour developing.(above each step all is to outwell after a last reagent uses up to add next reagent again) the 3rd step: observations: spottiness is positive, otherwise negative.
(3) combination three, Western blotting detects: the 1st step: fix each plate, the nitrocellulose filter 7+ cover plate 5 after the base plate 1+ transfer printing by assembling from the bottom to top.The 2nd step: in the hole of cover plate 5,, add confining liquid, enzyme labelled antibody, cleaning fluid, the substrate of enzyme, colour developing by successively.(above each step all is to outwell after a last reagent uses up to add next reagent again) the 3rd step: observations: coloured lines are represented purpose antigen in the test sample, otherwise does not have purpose antigen.
(4) combination four, the diafiltration of Western blotting gold detects: the 1st step: fix each plate, the nitrocellulose filter 7+ cover plate 5 after the base plate 1+ water accepting layer 8+ transfer printing by assembling from the bottom to top.The 2nd step: in the hole of cover plate 5, by successively, add confining liquid and infiltrate, add golden labeling antibody and infiltrate, add cleaning fluid and infiltrate, the 3rd step: observations: coloured lines are represented purpose antigen in the test sample, otherwise does not have purpose antigen.
Annotate: above enforcement combination one to four, if liquid is around the hole during seepage in point template or the cover board hole, will and its supporting silica gel plate be added between nitrocellulose membrane and above each plate, with antiseep.
Claims (10)
1, multi-functional high flux immunity detection reagent, by base plate (1), point template (2), point template (3), cover plate (4), cover plate (5) and silica gel plate (6), nitrocellulose filter (7), water accepting layer (8), micro-sampling pen, the gold labeling antibody, enzyme labelled antibody, the substrate of enzyme, confining liquid, cleaning fluid is formed, it is characterized by: when this kit used, it stacked order and is from the bottom to top: base plate (1), water accepting layer (8), nitrocellulose filter (7), point template (2) or point template (3), cover plate (4) or cover plate (5) are after the assembling, utensils such as available screw are fixing up and down with it, then reagent added in the hole on the cover plate.
2, multi-functional according to claim 1 high flux immunity detection reagent is characterized by: described cover plate (4): be 96 holes, and up/down perforation, its arrangement mode and sign 96 well culture plates together, the floorage in hole is 25-55mm
2
3, multi-functional according to claim 1 high flux immunity detection reagent is characterized by: described point template (2): be 96 holes, and up/down perforation, its arrangement mode is with 96 well culture plates, and the floorage in hole is 5-10mm
2, the position in each hole on it should be arranged in cover plate (4) and go up corresponding hole.
4, multi-functional according to claim 1 high flux immunity detection reagent is characterized by: described point template (3): be 192 holes, and up/down perforation, the floorage in hole is 3-4mm
2, per two Kong Weiyi group on it, the position in this group hole should be arranged in cover plate (4) and go up corresponding hole.
5, multi-functional according to claim 1 high flux immunity detection reagent is characterized by: described cover plate (5): hole is a strip hole on it, up/down perforation, and the floorage in hole is 20-40mm
2
6, multi-functional according to claim 1 high flux immunity detection reagent, it is characterized by: described base plate (1), point template (2), point template (3), cover plate (4), cover plate (5): all leave the fixedly pin hole of nitrocellulose filter and water accepting layer on four jiaos of each plate, by this hole with pin fixedly nitrocellulose filter and water accepting layer, make it when changing cover plate and point template, the holding position is constant.
7, multi-functional according to claim 1 high flux immunity detection reagent, it is characterized by: described silica gel plate,, do not have on its four jiaos the pin hole of fixing nitrocellulose filter or water accepting layer totally by 5, its shape respectively with base plate (1), point template (2), point template (3), cover plate (4), cover plate (5) is identical, and respectively with the supporting use of above each plate, place it between nitrocellulose filter and each plate, prevent the effect of liquid seepage around the hole in each plate hole.
8, multi-functional according to claim 1 high flux immunity detection reagent is characterized by: described micro-sampling pen: be kapillary.
9, multi-functional according to claim 1 high flux immunity detection reagent is characterized by: described nitrocellulose filter, its membrane aperture are 0.2-0.8 μ m.
10, multi-functional according to claim 1 high flux immunity detection reagent is characterized by: described kit, after its assembling, use screw, or spring clip, or the spring clasp is fixed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008200199528U CN201269877Y (en) | 2008-04-03 | 2008-04-03 | Multifunctional high flux immunity detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008200199528U CN201269877Y (en) | 2008-04-03 | 2008-04-03 | Multifunctional high flux immunity detection kit |
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| Publication Number | Publication Date |
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| CN201269877Y true CN201269877Y (en) | 2009-07-08 |
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| CNU2008200199528U Expired - Lifetime CN201269877Y (en) | 2008-04-03 | 2008-04-03 | Multifunctional high flux immunity detection kit |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102053154A (en) * | 2009-11-09 | 2011-05-11 | 深圳市宏信生物技术有限公司 | Kit for detecting autoimmune antibodies at throughput |
| CN102053153A (en) * | 2010-11-25 | 2011-05-11 | 西安微通生物技术有限公司 | Dot immuno gold directed infiltration detection kit and application thereof |
| CN103048472A (en) * | 2011-10-14 | 2013-04-17 | 深圳太太基因工程有限公司 | Using method of follicle-stimulating hormone colloidal gold assay kit |
| CN103091483A (en) * | 2013-01-11 | 2013-05-08 | 苏州浩欧博生物医药有限公司 | Production method of membrane diagnostic products |
| CN103399149A (en) * | 2013-08-22 | 2013-11-20 | 青岛中仁药业有限公司 | Family-planning four-item combined kit employing enzyme linked immunosorbent spot assay |
| CN109633153A (en) * | 2019-01-28 | 2019-04-16 | 浙江大学 | A kind of spot immune immunoblot method based on immune colloid gold of detection rice stripe mosaic virus and its application |
-
2008
- 2008-04-03 CN CNU2008200199528U patent/CN201269877Y/en not_active Expired - Lifetime
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102053154A (en) * | 2009-11-09 | 2011-05-11 | 深圳市宏信生物技术有限公司 | Kit for detecting autoimmune antibodies at throughput |
| CN102053153A (en) * | 2010-11-25 | 2011-05-11 | 西安微通生物技术有限公司 | Dot immuno gold directed infiltration detection kit and application thereof |
| CN103048472A (en) * | 2011-10-14 | 2013-04-17 | 深圳太太基因工程有限公司 | Using method of follicle-stimulating hormone colloidal gold assay kit |
| CN103091483A (en) * | 2013-01-11 | 2013-05-08 | 苏州浩欧博生物医药有限公司 | Production method of membrane diagnostic products |
| CN103399149A (en) * | 2013-08-22 | 2013-11-20 | 青岛中仁药业有限公司 | Family-planning four-item combined kit employing enzyme linked immunosorbent spot assay |
| CN109633153A (en) * | 2019-01-28 | 2019-04-16 | 浙江大学 | A kind of spot immune immunoblot method based on immune colloid gold of detection rice stripe mosaic virus and its application |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: YANTAI TUOPUBANG BIO-TECH CO., LTD. Free format text: FORMER OWNER: LUDONG UNIVERSITY Effective date: 20130130 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| C53 | Correction of patent for invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: Li Fenglian Inventor before: Wang Xiaojie |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 264025 YANTAI, SHANDONG PROVINCE TO: 264670 YANTAI, SHANDONG PROVINCE Free format text: CORRECT: INVENTOR; FROM: WANG XIAOJIE TO: LI FENGLIAN |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20130130 Address after: Three road 264670 in Shandong Province, Yantai high tech Zone Management Committee Wei No. 35 office building room 112 Patentee after: YANTAI TUOPUBANG BIOTECHNOLOGY CO., LTD. Address before: 264025 Hongqi Road, Zhifu District, Shandong, China, No. 186, No. Patentee before: Ludong University |
|
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20090708 |