CN109342741A - A kind of push-in blotting membrane clamping device - Google Patents
A kind of push-in blotting membrane clamping device Download PDFInfo
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- CN109342741A CN109342741A CN201811441575.1A CN201811441575A CN109342741A CN 109342741 A CN109342741 A CN 109342741A CN 201811441575 A CN201811441575 A CN 201811441575A CN 109342741 A CN109342741 A CN 109342741A
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- 239000012528 membrane Substances 0.000 title claims abstract description 53
- 239000000758 substrate Substances 0.000 claims abstract description 48
- 238000000429 assembly Methods 0.000 claims abstract description 20
- 230000000712 assembly Effects 0.000 claims abstract description 20
- 230000033228 biological regulation Effects 0.000 claims abstract description 17
- 238000003119 immunoblot Methods 0.000 claims description 12
- 238000003556 assay Methods 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 6
- 238000013519 translation Methods 0.000 claims description 4
- 238000000465 moulding Methods 0.000 claims description 3
- 239000000126 substance Substances 0.000 claims description 3
- 239000012085 test solution Substances 0.000 abstract description 20
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 238000001179 sorption measurement Methods 0.000 abstract description 3
- 238000010586 diagram Methods 0.000 description 13
- 230000009286 beneficial effect Effects 0.000 description 9
- 238000002474 experimental method Methods 0.000 description 6
- 230000007246 mechanism Effects 0.000 description 6
- 238000001262 western blot Methods 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 230000028993 immune response Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000010363 phase shift Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 230000035484 reaction time Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 102000018697 Membrane Proteins Human genes 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 238000005213 imbibition Methods 0.000 description 1
- 230000002998 immunogenetic effect Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
- G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Pathology (AREA)
- Microbiology (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Sampling And Sample Adjustment (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The present invention provides a kind of push-in blotting membrane clamping device, it include: aiding support component and clamp assemblies, aiding support component includes bottom plate and push plate, and clamp assemblies include clamping mainboard, top board and clamping piece, forms the regulation slot for inserting clamping piece between top board and clamping mainboard；Clamping piece includes substrate portion, flexible joint and the press keeping section being sequentially connected, blotting membrane is adsorbed on substrate portion surface by the adsorption capacity monolithic stability of moisture film, blotting membrane upper end is clamped between substrate portion and press keeping section, the part that blotting membrane enters test solution can be detached from substrate portion and sufficiently be reacted with test solution, and blotting membrane is detached from after test solution and meeting monolithic stability is adsorbed on substrate portion surface.Clamping piece manufacturing cost is greatly saved using being integrally formed in clamping piece；Putting need to only push after film push plate by one end push-in regulation slot of clamping piece, and the press keeping section of clamping piece is pressurized and by blotting membrane upper end stable holding, swift and convenient to operate, improve conventional efficient.
Description
Technical field
The present invention relates to a kind of experiment auxiliary appliance, especially a kind of push-in blotting membrane clamping device.
Background technique
Western blot test is also known as Western Blot Assay (Western Blot).It is biochemistry and immunogenetics
In a kind of common experimental method, be that the cell or tissue gross protein after electrophoretic separation is transferred to solid phase carrier from gel
On blotting membrane, then using on blotting membrane protein or polypeptide as antigen, play immune response with corresponding antibody, then with enzyme or same
The secondary antibody of position element label reacts, and the specific mesh of electrophoretic separation is finally detected by substrate colour developing or autoradiograph
Gene expression protein ingredient.In entire immune response experiment, need to carry out blotting membrane the cleaning-of more test solution multifrequencies time
Incubation-cleaning operation.
Western blot test is usually that sample is placed in reaction utensil in laboratory at present, in experimentation, by test solution
It is added in reaction utensil, test solution is individually sucked out by imbibition rifle again after reaction and is cleaned, in the primary antibody examination being rejected
In liquid and secondary antibody test solution includes the antibody not being consumed largely, causes the serious waste of primary antibody and secondary antibody, and primary antibody and two
Anti- price costly, results in higher experimental cost.Therefore a kind of blotting membrane of the market demand can be actively between each test solution
Mobile immunoblotting assay device, such primary antibody and secondary antibody test solution can reuse, but wherein how realize blotting membrane
Fast and stable clamping be primary urgent need to resolve project.
Summary of the invention
In order to overcome the above-mentioned deficiencies of the prior art, the object of the present invention is to provide a kind of push-in blotting membrane clamping dresses
It sets.
In order to achieve the above objectives, the technical solution adopted by the present invention to solve the technical problems is: a kind of push-in trace
Film clamping device, comprising:
Aiding support component comprising bottom plate and push plate, the plate upper surface include the first bearing surface and the second bearing
Face, the push plate are arranged on the first bearing surface of the bottom plate and can make anterior-posterior translation along the first bearing surface；
Clamp assemblies comprising the of the bottom plate is arranged in clamping mainboard, top board and clamping piece, the clamping mainboard
On two bearing surfaces, the top board is fixed on the clamping mainboard, is formed between the top board and clamping mainboard for inserting
Fill the regulation slot of the clamping piece；The clamping piece includes substrate portion, flexible joint and the press keeping section being sequentially connected, the pressure
The portion of holding can be in contact and separate with the substrate portion around flexible joint, and the press keeping section has the arc for generating tensile force
Curved sections, the clamping piece are made up of Shooting Technique integrated molding, and blotting membrane upper end is clamped in the substrate portion of the clamping piece
Between press keeping section, the bottom surface at substrate portion both ends is posted by the push plate upper surface and clamping mainboard surface respectively.
Compared to the prior art, blotting membrane is adsorbed on substrate portion surface by the adsorption capacity monolithic stability of moisture film to the present invention, prints
Mark film upper end is clamped between substrate portion and press keeping section, the part that blotting membrane enters test solution can be detached from substrate portion sufficiently and test solution into
Row reaction, blotting membrane is detached from after test solution and meeting monolithic stability is adsorbed on substrate portion surface；Clamping piece is using being integrally formed, greatly
Clamping piece manufacturing cost has been saved greatly；When putting film, the substrate subordinate bottom surface both ends of clamping piece are sticked on respectively in same plane
Push plate upper surface and clamping mainboard surface on, not will cause the deformation of blotting membrane, only push plate need to be pushed one end of clamping piece
It is pushed into regulation slot, the press keeping section of clamping piece is pressurized blotting membrane stable holding, and operation is more efficient and convenient, improves experiment effect
Rate；After the completion of blotting membrane clamping, clamp assemblies are removed from auxiliary support assemblies, are hanging to immunoblotting assay equipment
It on movable plate, is moved between each test solution slot by the reaction time, carries out cleaning-incubation-cleaning of multifrequency time, reduce antibody examination
The waste of liquid, has saved experimental cost.
Further, the both ends of the clamping mainboard are equipped with locating piece, and the bottom plate is equipped with and the locating piece phase
The locating slot matched, the lower surface of locating piece positioning column with outward extension, on the bottom plate positioning trench bottom be equipped with
The matched location hole of positioning column.
Using the above preferred scheme, clamp assemblies are improved and is placed into the position precision on aiding support component, clamping group
The pick-and-place of part is also very convenient, improves experimental implementation efficiency.
Further, the substrate portion of the clamping piece is equipped with the first guide groove of strip, and the clamping mainboard is equipped with
Guiding rib corresponding with the first guide groove；The top board is equipped with the second guide groove, sets on the press keeping section of the clamping piece
There is guide post corresponding with the second guide groove.
Using the above preferred scheme, cooperated by the first guide groove and guiding rib, stablize substrate portion in regulation slot
Translation；Cooperated by the second guide groove and guide post, makes press keeping section stably translational in regulation slot, it is ensured that clamping piece is steady by blotting membrane
Clamp is held.
Further, the press keeping section of the clamping piece face opposite with substrate portion is equipped with the limit extended to substrate portion
Column has the limit hole to match with the limited post in the substrate portion.
Using the above preferred scheme, when press keeping section pressurizes downwards, limited post and limit hole cooperation, it is ensured that press keeping section
Position of the head end in substrate portion keeps stablizing, and prevents moving for blotting membrane.
Further, the root of the second guide groove of the top board is equipped with the hooking hole being recessed to bottom surface, in the folder
When holding piece and plugging regulation slot root, the guide post enters in the hooking hole.
Using the above preferred scheme, after clamping piece plugs together in place, guide post enters in hooking hole, can prevent clamping piece
Deviate from easily, improves the clamping stable degree of blotting membrane.
Further, the hooking hole penetrates into the upper surface of the top board, and the hooking hole is in top board upper surface
Oral area at indent circular conical surface.
Using the above preferred scheme, it after the completion of immunoblot experiment, when needing to take out clamping piece and blotting membrane, only needs
It to press guide post downwards from top board upper surface, then can easily extract clamping piece out of regulation slot out；Indent circular conical surface side
Just operator directly presses with finger, and improves operation ease.
Further, magnetic substance is embedded on the binding face of the clamping mainboard and bottom plate.
Using the above preferred scheme, keep certain the suction-combining force between clamping mainboard and the bottom plate, prevent slightly by
Phase shift both after power；It slightly firmly can be taken off clamp assemblies after blotting membrane clamping, improve operation ease.
Further, the push plate is equipped with positioning pin, and the head in clamping piece substrate portion is equipped with to be matched with positioning pin
Semicircular opening.
Using the above preferred scheme, the one end in clamping piece substrate portion is cooperated by the first guide groove and guiding rib, another
End improves the smoothness in clamping piece moving process by semicircular opening and detent fit.
Further, it is additionally provided on the clamping mainboard for the clamp assemblies to be hanging to immunoblotting assay equipment
On suspention ear, the suspention ear be equipped with mounting hole.
Using the above preferred scheme, clamp assemblies are improved and is installed to the convenience in immunoblotting assay equipment.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with
It obtains other drawings based on these drawings.
Fig. 1 is the structural schematic diagram of one embodiment of the present invention；
Fig. 2 is the structural schematic diagram of one embodiment of the present invention；
Fig. 3 is the structural schematic diagram of aiding support component another embodiment of the present invention；
Fig. 4 is the structural schematic diagram of present invention clamping mainboard and top board bonding state；
Fig. 5 is a kind of present invention clamping structural schematic diagram of embodiment of mainboard；
Fig. 6 is a kind of structural schematic diagram of embodiment of top board of the present invention；
Fig. 7 is the structural schematic diagram of top board another embodiment of the present invention；
Fig. 8 is a kind of structural schematic diagram of embodiment of clamping piece of the present invention；
Fig. 9 is a kind of structural schematic diagram of embodiment of clamping piece of the present invention；
Figure 10 is a kind of structural schematic diagram of embodiment of clamping piece of the present invention；
Figure 11 is the structural schematic diagram of immunoblotting assay equipment.
The title of number and corresponding component represented by letter in figure:
1- aiding support component；11- bottom plate；The first bearing surface of 111-；The second bearing surface of 112-；113- locating slot；114-
Location hole；12- push plate；121- positioning pin；2- clamp assemblies；21- clamps mainboard；211- regulation slot；212- locating piece；213- is fixed
Position column；214- guiding rib；215- mounting hole；22- top board；The second guide groove of 221-；222- hooking hole；223- indent circular cone
Face；23- clamping piece；231- substrate portion；232- flexible joint；233- press keeping section；The first guide groove of 234-；235- guide post；
236- limited post；237- limit hole；238- semicircular opening；3- is moved forward and backward mechanism；4- reciprocating mechanism；5- movable plate；6-
Test solution box.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
As Figure 1-10 shows, a kind of push-in blotting membrane clamping device, comprising:
A kind of push-in blotting membrane clamping device, comprising:
Aiding support component 1 comprising bottom plate 11 and push plate 12,11 upper surface of bottom plate include the first bearing surface 111 and the
Two bearing surfaces 112, push plate 12 are arranged on the first bearing surface 111 of bottom plate 11 and can make anterior-posterior translation along the first bearing surface 111；
Clamp assemblies 2 comprising clamping mainboard 21, top board 22 and clamping piece 23, clamping mainboard 21 are arranged in bottom plate 11
The second bearing surface 112 on, top board 22 is fixed on clamping mainboard 21, is formed and is used between top board 22 and clamping mainboard 21
Insert the regulation slot 211 of clamping piece 23；Clamping piece 23 includes substrate portion 231, flexible joint 232 and the press keeping section being sequentially connected
233, press keeping section 233 can be in contact and separate with substrate portion 231 around flexible joint 232, and press keeping section 233 has to be opened for generating
The arc-shaped curved surface section of clamp force, clamping piece 23 are made up of Shooting Technique integrated molding, and blotting membrane upper end is clamped in clamping piece 23
Between substrate portion 231 and press keeping section 233, the bottom surface at 231 both ends of substrate portion is posted by 12 upper surface of push plate and clamping master respectively
21 surface of plate.
Beneficial effect by adopting the above technical scheme is: blotting membrane is adsorbed on substrate portion by the adsorption capacity monolithic stability of moisture film
231 surfaces, blotting membrane upper end are clamped between substrate portion 231 and press keeping section 233, and the part that blotting membrane enters test solution can be detached from base
Piece portion is sufficiently reacted with test solution, and blotting membrane is detached from after test solution and meeting monolithic stability is adsorbed on substrate portion surface；Clamping piece 23
Using being integrally formed, clamping piece manufacturing cost is greatly saved；When putting film, the 231 bottom surface both ends of substrate portion of clamping piece
It is sticked on conplane push plate upper surface and clamping mainboard surface respectively, not will cause the deformation of blotting membrane, only needed
Push push plate 12 by one end push-in regulation slot of clamping piece 23, the press keeping section of clamping piece is pressurized blotting membrane stable holding, Fig. 1
Clamping piece 23 is shown and is pushed into preceding status diagram, the status diagram after being pushed into Fig. 2 shows clamping piece 23, side swift to operate
Just, conventional efficient is improved.As shown in figure 11, a kind of immunoblotting assay equipment, including be moved forward and backward mechanism 3, move up and down
Mechanism 4, movable plate 5 and multiple test solution boxes 6 move up clamp assemblies 2 from aiding support component 1 after the completion of blotting membrane clamping
Out, stabilization is hanging on the movable plate 5 of immunoblotting assay equipment, is moved forward and backward mechanism 3 and reciprocating mechanism 4 matches band
Blotting membrane on dynamic clamp assemblies is moved between each test solution slot by the reaction time, carries out cleaning-incubation-cleaning of multifrequency time,
The waste for reducing antibody test solution, has saved experimental cost.
As shown in Fig. 3,5, in other embodiments of the invention, the both ends of clamping mainboard 21 are equipped with locating piece 212,
Bottom plate 11 is equipped with the locating slot 113 to match with locating piece 212, the positioning with outward extension of the lower surface of locating piece 212
Column 213,113 bottom of locating slot is equipped with and the matched location hole 114 of positioning column 213 on bottom plate.Having by adopting the above technical scheme
Beneficial effect is: it improves clamp assemblies and is placed into the position precision on aiding support component, the pick-and-place of clamp assemblies is also very convenient,
Improve experimental implementation efficiency.
As shown in Fig. 5,6,9,10, in other embodiments of the invention, the substrate portion 231 of clamping piece 23 is equipped with length
First guide groove 234 of bar shaped, clamping mainboard 21 are equipped with guiding rib 214 corresponding with the first guide groove 234；Top board 22
It is equipped with the second guide groove 221, the press keeping section 233 of clamping piece is equipped with guide post 235 corresponding with the second guide groove 221.It adopts
It is with the beneficial effect of above-mentioned technical proposal: is cooperated by the first guide groove 234 and guiding rib 214, make substrate portion in regulation slot
Interior stably translational；Cooperated by the second guide groove 221 and guide post 235, make press keeping section stably translational in regulation slot, it is ensured that clamping
Piece is by blotting membrane stable holding.
As shown in Fig. 8,10, in other embodiments of the invention, the press keeping section 233 of clamping piece is opposite with substrate portion
Face be equipped with the limited post 236 that extends to substrate portion, substrate portion 231 is equipped with the limit hole to match with limited post 236
237.Beneficial effect by adopting the above technical scheme is: when press keeping section pressurizes downwards, limited post and limit hole cooperation, it is ensured that pressure
It holds position of the head end in portion in substrate portion to keep stablizing, prevents moving for blotting membrane.
As shown in fig. 7, the root of second guide groove 221 of top board 22 is set in other embodiments of the invention
The hooking hole 222 of oriented bottom surface recess, when clamping piece plugs regulation slot root, guide post 235 enters in hooking hole 222.It adopts
Be with the beneficial effect of above-mentioned technical proposal: after clamping piece plugs together in place, guide post enters in hooking hole, can prevent clamping piece
Deviate from easily, improves the clamping stable degree of blotting membrane.
As shown in fig. 7, hooking hole 222 penetrates into the upper surface of top board 22 in other embodiments of the invention,
Hooking hole 222 22 upper surface of top board oral area at indent circular conical surface 223.Beneficial effect by adopting the above technical scheme is:
After the completion of immunoblot experiment, when needing to take out clamping piece and blotting membrane, it is only necessary to press downwards and lead from top board upper surface
Then column can easily extract clamping piece out out of regulation slot；Indent circular conical surface facilitates operator directly to press with finger, and mentions
High operation ease.
In other embodiments of the invention, magnetic substance is embedded on the binding face of clamping mainboard 21 and bottom plate 11.
Beneficial effect by adopting the above technical scheme is: keeping certain the suction-combining force between clamping mainboard and bottom plate, prevents slight stress
The two phase shift afterwards；It slightly firmly can be taken off clamp assemblies after blotting membrane clamping, improve operation ease.
As shown in Fig. 3,9, in other embodiments of the invention, push plate 12 is equipped with positioning pin 121, clamps chip base
The head in piece portion 231 is equipped with and the matched semicircular opening 238 of positioning pin 121.Beneficial effect by adopting the above technical scheme is: folder
The one end for holding piece substrate portion is cooperated by the first guide groove 234 and guiding rib 214, and the other end passes through semicircular opening 238 and positioning
Pin 121 cooperates, and improves the smoothness in clamping piece moving process.
As shown in figure 5, in other embodiments of the invention, clamps and be additionally provided on mainboard 21 for by clamp assemblies
2 are hanging to the suspention ear in immunoblotting assay equipment, and the suspention ear is equipped with mounting hole 215.By adopting the above technical scheme
Beneficial effect be: improve clamp assemblies and be installed to convenience in immunoblotting assay equipment.
The above embodiments merely illustrate the technical concept and features of the present invention, and its object is to allow ordinary skill people
Member can understand the contents of the present invention and be implemented, it is not intended to limit the scope of the present invention, it is all according to the present invention
Equivalent change or modification made by Spirit Essence, should be covered by the scope of protection of the present invention.
Claims (9)
1. a kind of push-in blotting membrane clamping device characterized by comprising
Aiding support component comprising bottom plate and push plate, the plate upper surface include the first bearing surface and the second bearing surface, institute
Push plate is stated to be arranged on the first bearing surface of the bottom plate and anterior-posterior translation can be made along the first bearing surface；
Clamp assemblies comprising second of the bottom plate is arranged in clamping mainboard, top board and clamping piece, the clamping mainboard
In bearing surface, the top board is fixed on the clamping mainboard, is formed between the top board and clamping mainboard for inserting
The regulation slot of clamping piece is stated, the clamping piece includes substrate portion, flexible joint and the press keeping section being sequentially connected, the press keeping section
It can be in contact and separate with the substrate portion around flexible joint, the press keeping section has the arc-shaped curved surface for generating tensile force
Section, the clamping piece are made up of Shooting Technique integrated molding, and blotting membrane is integrally adsorbed on substrate portion surface, blotting membrane upper end folder
It holds between substrate portion and press keeping section.
2. push-in blotting membrane clamping device according to claim 1, which is characterized in that the both ends of the clamping mainboard are set
There is locating piece, the bottom plate is equipped with the locating slot to match with the locating piece, and the lower surface of the locating piece is equipped with outside
The positioning column of extension, positioning trench bottom is equipped with and the matched location hole of the positioning column on the bottom plate.
3. push-in blotting membrane clamping device according to claim 1, which is characterized in that the substrate portion of the clamping piece is set
There is the first guide groove of strip, the clamping mainboard is equipped with guiding rib corresponding with the first guide groove；The top board
It is equipped with the second guide groove, the press keeping section of the clamping piece is equipped with guide post corresponding with the second guide groove.
4. push-in blotting membrane clamping device according to claim 3, which is characterized in that the press keeping section of the clamping piece with
The opposite face in substrate portion is equipped with the limited post extended to substrate portion, has the limit to match with the limited post in the substrate portion
Position hole.
5. push-in blotting membrane clamping device according to claim 4, which is characterized in that the second guiding of the top board
The root of slot is equipped with the hooking hole being recessed to bottom surface, and when the clamping piece plugs regulation slot root, the guide post enters institute
It states in hooking hole.
6. push-in blotting membrane clamping device according to claim 5, which is characterized in that the hooking hole penetrates into described
The upper surface of top board, the hooking hole top board upper surface oral area at indent circular conical surface.
7. push-in blotting membrane clamping device according to claim 1, which is characterized in that the clamping mainboard and bottom plate
Magnetic substance is embedded on binding face.
8. push-in blotting membrane clamping device according to claim 7, which is characterized in that the push plate is equipped with positioning
The head of pin, clamping piece substrate portion is equipped with and the matched semicircular opening of positioning pin.
9. push-in blotting membrane clamping device according to claim 8, which is characterized in that be additionally provided on the clamping mainboard
Suspention ear for being hanging to the clamp assemblies in immunoblotting assay equipment, the suspention ear are equipped with mounting hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811441575.1A CN109342741B (en) | 2018-11-29 | 2018-11-29 | Quick-insertion type imprinting film clamping device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811441575.1A CN109342741B (en) | 2018-11-29 | 2018-11-29 | Quick-insertion type imprinting film clamping device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109342741A true CN109342741A (en) | 2019-02-15 |
| CN109342741B CN109342741B (en) | 2024-02-23 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811441575.1A Active CN109342741B (en) | 2018-11-29 | 2018-11-29 | Quick-insertion type imprinting film clamping device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109900912A (en) * | 2019-03-19 | 2019-06-18 | 莫纳（苏州）生物科技有限公司 | A kind of clamping type immunoblotting assay instrument and its control system |
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