CN207408417U - A kind of Western blotting exposure box - Google Patents
A kind of Western blotting exposure box Download PDFInfo
- Publication number
- CN207408417U CN207408417U CN201721432540.2U CN201721432540U CN207408417U CN 207408417 U CN207408417 U CN 207408417U CN 201721432540 U CN201721432540 U CN 201721432540U CN 207408417 U CN207408417 U CN 207408417U
- Authority
- CN
- China
- Prior art keywords
- clamp plate
- plate
- lower clamp
- magazine
- western blotting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Abstract
The utility model discloses a kind of Western blotting exposure box, including：Magazine, including a magazine body, the magazine cover being covered at magazine body openings；Fluorescent marker clamping plate, the fluorescent marker clamping plate is built in magazine body, including upper clamp plate, lower clamp plate, connecting portion；The upper clamp plate arranges that the one side of the upper clamp plate and lower clamp plate is hinged with connecting portion with lower clamp plate up and down, and the upper clamp plate is transparent panel, and the lower clamp plate upper surface is provided with fluorescence scales line；Pressing plate, the pressing plate are overlaid on upper clamp plate upper surface.When the utility model is exposed protein solid phase carrier, protein solid phase carrier is placed between the upper and the lower plywood, and developed film is placed on upper clamp plate upper surface, avoids protein solid phase carrier and developed film adhesion；The lower clamp plate upper surface is provided with fluorescence scales line, and graduation mark and protein can be exposed on developed film after having developed, consequently facilitating determining the position of albumen development and size on developed film.
Description
Technical field
The utility model is related to a kind of biotechnological device field, more particularly, to a kind of Western blotting exposure box.
Background technology
Protein immunoblot experiment is than a kind of more conventional experimental method in current laboratory, and secondary antibody shines after being incubated
It is a very crucial step to identify this step, directly affects the quality of experiment picture.A kind of full-automatic Western blotting is provided on the market
Instrument, but its price is not low, and the laboratory for shortage of funding is simultaneously undesirable.Usual laboratory can be pressed using a kind of tabletting magazine
Piece, although cost is very low, this tabletting magazine in use, protein solid phase carrier always with cohesion of film together with, such as：
Nitrocellulose filter (NC films) or polyvinylidene fluoride film (pvdf membrane) always with cohesion of film together with, it is extremely inconvenient when removing and
And the movement of film can be also caused, cause the error of experimental result.In addition on film egg is not can determine that after having developed without reference to object
The size of informal voucher band.
Utility model content
The purpose of the utility model is to overcome above-mentioned technical deficiencies, propose a kind of Western blotting exposure box, solve existing
There is the tabletting magazine in technology in use, nitrocellulose filter (NC films) or polyvinylidene fluoride film (pvdf membrane) are always glued with film
It connects together, it is extremely inconvenient when removing but also the movement of film can be caused, cause the error of experimental result.In addition do not have on film
Object of reference, the technical issues of not can determine that the size of protein band after having developed.
To reach above-mentioned technical purpose, the technical solution of the utility model provides a kind of Western blotting exposure box, including：
Magazine, including a magazine body, the magazine cover being covered at magazine body openings；
Fluorescent marker clamping plate, the fluorescent marker clamping plate is built in magazine body, including upper clamp plate, lower clamp plate, connection
Portion；The upper clamp plate arranges that the one side of the upper clamp plate and lower clamp plate is hinged with connecting portion, the upper folder with lower clamp plate up and down
Plate is transparent panel, and the lower clamp plate upper surface is provided with fluorescence scales line；
Pressing plate, the pressing plate are overlaid on upper clamp plate upper surface.
Compared with prior art, the beneficial effects of the utility model include：The Western blotting exposure box, to protein
When solid phase carrier is exposed, protein solid phase carrier is placed between the upper and the lower plywood, and developed film is placed on upper clamp plate upper table
Face so as to which protein solid phase carrier, developed film are separated, avoids protein solid phase carrier and developed film adhesion；It is described
Lower clamp plate upper surface is provided with fluorescence scales line, and graduation mark and protein can be exposed on developed film after having developed, so as to
Convenient for determining the position of albumen development and size on developed film.
Description of the drawings
Fig. 1 is the volume rendering schematic diagram of the utility model.
Fig. 2 is the schematic front perspective view of the utility model.
Fig. 3 is the close-up schematic view of Fig. 2.
Fig. 4 is the fluorescent marker clamping plate dimensional structure diagram of the utility model.
Specific embodiment
In order to make the purpose of the utility model, technical solutions and advantages more clearly understood, below in conjunction with attached drawing and implementation
Example, is further elaborated the utility model.It should be appreciated that specific embodiment described herein is only used to explain
The utility model is not used to limit the utility model.
~4 are please referred to Fig.1, the utility model provides a kind of Western blotting exposure box, including magazine 1, fluorescent marker
Clamping plate 2, pressing plate 3.
Magazine 1, the magazine 1 is a black plastic quadrangle box, including a magazine body 11, magazine cover 12；Institute
State the upper opening that magazine cover 12 is fastened on magazine body 11.
Fluorescent marker clamping plate 2, the fluorescent marker clamping plate 2 is square, and is built in magazine body 11, including upper clamp plate
21st, lower clamp plate 22, connecting portion 23；The one side of the upper clamp plate 21 and lower clamp plate 22 is hinged by connecting portion 23, specifically, described
Upper clamp plate 21, lower clamp plate 22, connecting portion 23 are bendable material and integrated molding, and the upper clamp plate 21, lower clamp plate 22 are with connecting
The link position of socket part 23 is both provided with to folding line, and the bending part 23 is circular arc type, is exposed to protein solid phase carrier 4
Light time, protein solid phase carrier 4 are placed between the upper and the lower plywood, and developed film 5 is placed on 21 upper surface of upper clamp plate, so as to egg
It is separated between white matter solid phase carrier 4 and developed film 5 by upper clamp plate 21, avoids protein solid phase carrier 4 and developed film 5 is viscous
Even；The upper clamp plate 21 is transparent panel, and 22 upper surface of lower clamp plate is provided with fluorescence scales line 22a, the fluorescence scales line
22a include a first graduation mark 22a1 arranged along 22 length direction of lower clamp plate, one arranged along 22 width of lower clamp plate the
Two graduation mark 22a2, graduation mark and protein can be exposed on developed film 5 after having developed, according to graduation mark, convenient for determining
The position and size that albumen develops on developed film 5, specifically, the first graduation mark 22a1, the second graduation mark 22a2 distinguish
Long side, broadside arrangement close to lower clamp plate 22.
Pressing plate 3, the pressing plate 3 are provided with the square glass plate of arc chord angle for seamed edge, and the pressing plate 3 is built in magazine body
11, and 21 upper surface of upper clamp plate is overlaid on, make developed film 5 and fluorescent marker clamping plate 2 and its protein solid phase carrier 4 of inside
It mutually compresses, ensures exposure effect.
The operation principle of the utility model：During using the Western blotting exposure box, user needs that fluorescent marker is first unfolded
Clamping plate 2, being positioned over 22 upper surface of lower clamp plate by the processed protein solid phase carrier 4 of developer solution and do not cover fluorescence scales
Line 22a, then reversion fastens upper clamp plate 21, then pressing plate 3 is overlaid on 21 upper surface of upper clamp plate, makes developed film 5 and fluorescence mark
Remember that the protein solid phase carrier 4 of clamping plate 2 and its inside mutually compresses, magazine cover 12 is finally fastened on the upper of magazine body 11
Portion's opening, develops；Between being placed on the upper and the lower plywood due to protein solid phase carrier 4, developed film 5 is covered in upper clamp plate
21 upper surfaces, so as to avoid 5 adhesion of protein solid phase carrier 4 and developed film；22 upper surface of lower clamp plate is provided with glimmering
Light graduation mark 22a, graduation mark and protein can be exposed on developed film 5 after having developed, consequently facilitating determining developed film 5
The position of upper albumen development and size.
Specific embodiment of the present utility model described above does not form the restriction to scope of protection of the utility model.
The various other corresponding changes and deformation that any technical concept according to the present utility model is made, should be included in this practicality
In new scope of the claims.
Claims (6)
1. a kind of Western blotting exposure box, including：
Magazine, including a magazine body, the magazine cover being covered at magazine body openings；
Fluorescent marker clamping plate, the fluorescent marker clamping plate is built in magazine body, including upper clamp plate, lower clamp plate, connecting portion；Institute
It states upper clamp plate and arranges that the one side of the upper clamp plate and lower clamp plate is hinged with connecting portion, and the upper clamp plate is up and down with lower clamp plate
Transparent panel, the lower clamp plate upper surface are provided with fluorescence scales line；
Pressing plate, the pressing plate are overlaid on upper clamp plate upper surface.
2. Western blotting exposure box according to claim 1, which is characterized in that the upper clamp plate, lower clamp plate, connecting portion
It is bendable material and integrated molding, the link position of the upper clamp plate, lower clamp plate and connecting portion is both provided with to folding line.
3. Western blotting exposure box according to claim 2, which is characterized in that the bending part is circular arc type.
4. Western blotting exposure box according to claim 1, which is characterized in that the magazine, fluorescent marker clamping plate, pressure
Plate is square；The pressing plate is a glass plate, and the seamed edge of pressing plate is provided with arc chord angle.
5. Western blotting exposure box according to claim 4, which is characterized in that the fluorescence scales line includes one under
First graduation mark of clamping plate length direction arrangement, second graduation mark arranged along lower clamp plate width.
6. Western blotting exposure box according to claim 5, which is characterized in that first graduation mark, the second scale
Long side, broadside arrangement of the line respectively close to lower clamp plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721432540.2U CN207408417U (en) | 2017-10-31 | 2017-10-31 | A kind of Western blotting exposure box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721432540.2U CN207408417U (en) | 2017-10-31 | 2017-10-31 | A kind of Western blotting exposure box |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207408417U true CN207408417U (en) | 2018-05-25 |
Family
ID=62409267
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721432540.2U Expired - Fee Related CN207408417U (en) | 2017-10-31 | 2017-10-31 | A kind of Western blotting exposure box |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207408417U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109342741A (en) * | 2018-11-29 | 2019-02-15 | 莫纳(连云港)生物科技有限公司 | A kind of push-in blotting membrane clamping device |
| WO2020192330A1 (en) * | 2019-03-28 | 2020-10-01 | 上海易孛特光电技术有限公司 | Photoelectric conversion detection device and packaging method for same |
| CN114326321A (en) * | 2021-12-13 | 2022-04-12 | 复旦大学附属中山医院 | Auxiliary exposure device for western blot strip |
-
2017
- 2017-10-31 CN CN201721432540.2U patent/CN207408417U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109342741A (en) * | 2018-11-29 | 2019-02-15 | 莫纳(连云港)生物科技有限公司 | A kind of push-in blotting membrane clamping device |
| CN109342741B (en) * | 2018-11-29 | 2024-02-23 | 莫纳(武汉)生物科技有限公司 | Quick-insertion type imprinting film clamping device |
| WO2020192330A1 (en) * | 2019-03-28 | 2020-10-01 | 上海易孛特光电技术有限公司 | Photoelectric conversion detection device and packaging method for same |
| CN114326321A (en) * | 2021-12-13 | 2022-04-12 | 复旦大学附属中山医院 | Auxiliary exposure device for western blot strip |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180525 Termination date: 20181031 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |