CN214585479U - Fluorescent microsphere lateral chromatography detection card for detecting novel coronavirus antibody - Google Patents
Fluorescent microsphere lateral chromatography detection card for detecting novel coronavirus antibody Download PDFInfo
- Publication number
- CN214585479U CN214585479U CN202120852295.0U CN202120852295U CN214585479U CN 214585479 U CN214585479 U CN 214585479U CN 202120852295 U CN202120852295 U CN 202120852295U CN 214585479 U CN214585479 U CN 214585479U
- Authority
- CN
- China
- Prior art keywords
- novel coronavirus
- box body
- detection card
- detecting
- chromatography detection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The utility model discloses a detect fluorescence microballon side direction chromatography detection card of novel coronavirus antibody aims at improving the sensitivity that new coronavirus antibody detected, its technical scheme: the kit comprises a bottom plate, wherein a sample pad, a combination pad, a nitrocellulose membrane and an absorption pad are sequentially connected to the bottom plate from left to right, and the combination pad is loaded with staphylococcal protein A marked by fluorescent microspheres; the nitrocellulose membrane is parallelly provided with a detection line and a quality control line, the detection line is coated with a novel coronavirus N protein, and the quality control line is coated with chicken IgY, and belongs to the technical field of detection.
Description
Technical Field
The utility model belongs to the technical field of detect, more specifically, relate to a detect fluorescence microballon side direction chromatography detection card of novel coronavirus antibody.
Background
The clinical symptoms of the novel coronavirus pneumonia are fever, hypodynamia, dry cough and gradual dyspnea, and severe patients show acute respiratory distress syndrome, septic shock, metabolic acidosis and blood coagulation dysfunction which are difficult to correct. The latent period of the disease has the characteristics of infectivity, strong interpersonal infectivity and the like.
The invention discloses a new coronavirus antibody detection card and a manufacturing method thereof, wherein the detection card comprises a test strip packaged in a shell, and the shell comprises an observation hole and a sample adding hole; the test strip comprises a bottom plate, a sample pad, a gold label pad, a nitrocellulose membrane and a water absorption membrane; the gold label pad is sprayed with a colloidal gold-SPA solution and a colloidal gold-mouse anti-human IgG solution; the nitrocellulose membrane is provided with detection lines and quality control lines at intervals in parallel, the detection lines are coated with novel coronavirus S protein and N protein, and the quality control lines are coated with rabbit anti-sheep secondary antibody.
In the scheme, the novel coronavirus antibody is detected by a colloidal gold immunochromatography technology, and the sensitivity is low.
Therefore, the technical problems to be solved by the application are as follows: how to improve the sensitivity of the detection of the new coronavirus antibody.
SUMMERY OF THE UTILITY MODEL
The main object of the present invention is to provide a fluorescent microsphere lateral chromatography detection card for detecting novel coronavirus antibodies, which aims to improve the sensitivity of detecting novel coronavirus antibodies.
According to the first aspect of the utility model, the utility model provides a fluorescent microsphere lateral chromatography detection card for detecting novel coronavirus antibody, which comprises a bottom plate, wherein a sample pad, a combination pad, a nitrocellulose membrane and an absorption pad are sequentially connected on the bottom plate from left to right, and the combination pad is loaded with a staphylococcal protein A marked by fluorescent microsphere; the nitrocellulose membrane is provided with a detection line and a quality control line in parallel, the detection line is coated with a novel coronavirus N protein, and the quality control line is coated with chicken IgY.
In a specific embodiment of the present invention, the utility model further comprises a box body, wherein the bottom plate is fixed in the box body;
the box body is also provided with a sample adding hole corresponding to the sample pad and an observation window corresponding to the detection line and the quality control line on the nitrocellulose membrane.
In a specific embodiment of the present invention, the sample adding hole is provided with a cover plate which can be opened and closed; the observation window is provided with a transparent panel.
In a specific embodiment of the present invention, a sliding groove is formed on the box body, the cover plate is slidably connected in the sliding groove, and the cover plate slides in the sliding groove to open and close the sample adding hole.
In a specific embodiment of the present invention, a concave portion is disposed on a side of an end of the chute away from the sample adding hole, and an elastic shifting piece is disposed in the concave portion and extends obliquely in a direction away from the sample adding hole; a concave cavity matched with the elastic shifting piece is arranged on the side wall of the cover plate;
the cover plate slides, the elastic shifting piece is tightly propped against the side edge of the cover plate to generate deformation, and the shape of the elastic shifting piece is recovered and extends into the concave cavity after meeting the concave cavity.
In a specific embodiment of the present invention, a side of the cover plate away from the box body is provided with an anti-slip structure.
In a particular embodiment of the invention, a detachable straw is provided on one side of the box body.
In a specific embodiment of the present invention, an elastic snap ring is disposed on one side of the box body, an opening for the straw to enter is disposed on the elastic snap ring, and the straw is detachably connected to the elastic snap ring.
In a specific embodiment of the present invention, a limiting groove for limiting the position of the straw is further disposed on one side of the box body, which is provided with the elastic snap ring, and the limiting groove is provided with an opening portion for the straw to enter;
the elastic clamping ring and the limiting groove are respectively arranged at two ends of the box body in the length direction.
In a specific embodiment of the present invention, the snap ring and the limiting groove are both disposed on a mounting plate, the box body is provided with a mounting groove for mounting the mounting plate, one end of the mounting groove is for supplying the mounting plate with an inserted open end, and the mounting plate is detachably connected to the mounting groove.
The utility model discloses a technical scheme has following advantage or one of beneficial effect at least among the above-mentioned technical scheme:
in the utility model, the combination pad is loaded with staphylococcus protein A marked by fluorescent microspheres; the nitrocellulose membrane is provided with a detection line and a quality control line in parallel, the detection line is coated with a novel coronavirus N protein, and the quality control line is coated with chicken IgY;
the fluorescent microsphere lateral chromatography technology is used for detecting the new coronavirus antibody, so that the detection sensitivity is effectively improved.
Drawings
The present invention will be further described with reference to the accompanying drawings and examples;
fig. 1 is a structural view of a base plate of a first embodiment of the present invention;
fig. 2 is a top view structural diagram of the cartridge body according to the first embodiment of the present invention;
FIG. 3 is a top view of the first embodiment of the present invention with the cover removed;
fig. 4 is an exploded view of the mounting groove of the first embodiment of the present invention in cooperation with the mounting plate.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the accompanying drawings are exemplary only for the purpose of explaining the present invention, and should not be construed as limiting the present invention.
In the description of the present invention, it should be understood that the orientation or positional relationship indicated with respect to the orientation description, such as up, down, front, rear, left, right, etc., is based on the orientation or positional relationship shown in the drawings, and is only for convenience of description and simplification of description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the term "connected" is to be interpreted broadly, and may be, for example, a fixed connection or a movable connection, a detachable connection or a non-detachable connection, or an integral connection; may be mechanically connected, may be electrically connected or may be in communication with each other; they may be directly connected or indirectly connected through intervening media, or may be connected through one or more other elements or indirectly connected through one or more other elements or in an interactive relationship between two elements.
The following disclosure provides many different embodiments or examples for implementing different aspects of the invention.
Referring to fig. 1 to 4, a fluorescent microsphere lateral chromatography detection card for detecting a novel coronavirus antibody comprises a bottom plate 1, wherein a sample pad 11, a binding pad 12, a nitrocellulose membrane 13 and an absorption pad 14 are sequentially connected to the bottom plate 1 from left to right, and staphylococcal protein a121 marked by a fluorescent microsphere is loaded on the binding pad 12; the nitrocellulose membrane 13 is provided with a detection line T and a quality control line C in parallel, the detection line T is coated with a novel coronavirus N protein, and the quality control line C is coated with chicken IgY.
The fluorescent microsphere lateral chromatography is a novel quantitative detection technology which is developed and innovated by combining an immunofluorescence technology and a traditional immunochromatography technology, the technology realizes accurate quantification of a detection result through a fluorescent tracing enhancement technology besides the advantages of simple and convenient operation, quick detection and strong portability of a colloidal gold immunochromatography technology, and compared with the performance of the traditional quick detection technology, the technology has the advantages of higher sensitivity, wider detection range, lower price and the like.
In the embodiment, the fluorescent microsphere lateral chromatography technology is used for detecting the new coronavirus antibody, so that the detection sensitivity is effectively improved.
Specifically, the box body 2 is further included, and the bottom plate 1 is fixed in the box body 2;
the box body 2 is also provided with a sample adding hole 21 corresponding to the sample pad 11 and an observation window 22 corresponding to the detection line T and the quality control line C on the nitrocellulose membrane 13.
When detecting the new coronavirus antibody, sucking 10 mul of sample to be detected, adding the sample to the sample diluent, and uniformly mixing. Sucking 100 mul of mixed sample, adding the sample into a sample adding hole 21 of the detection card, stably inserting the sample into a card slot of the instrument (the end of the sample adding hole 21 faces to the inlet of the card slot), pushing to the end, clicking a 'measurement' option, starting detection, displaying and printing a result. Judging the result that the antibody is negative when the antibody is less than 10TU/ml, and judging the antibody is positive otherwise;
the fluorescent microsphere lateral chromatography technology is used for detecting the new coronavirus antibody, so that detection result data can be stored, and the batch export is convenient to return to consideration;
the detection card detects inactivated serum of 6 novel coronavirus confirmed patients in different periods, including early serum, middle serum and late serum of the patients, and the result shows that the early serum of the 6 confirmed patients cannot be detected, part of the middle serum is detected, and all the late serum is detected, so that the detection card is proved to have good reactivity with the new coronavirus positive serum.
Meanwhile, the detection card is used for detecting the coronavirus and the porcine coronavirus of the rat and the mouse, the results are negative, the specificity is good, and no cross reaction is caused between the detection card and the coronavirus and the porcine coronavirus;
the detection process and the result show that the detection card is suitable for detecting serum antibodies of multiple species such as human, monkey, pig, rabbit, mouse and the like, and has the potential of being put into large-scale multi-species antibody screening work in new crown traceability research.
In this embodiment, the sample adding hole 21 is provided with an openable cover plate 3, when the detection card is not used, the cover plate 3 closes the sample adding hole 21 to prevent foreign matters from entering the box body 2 from the sample adding hole 21, so as to prevent the detection card from being polluted; when the detection card is required to be used for detection, the cover plate 3 is opened, and a sample is added from the sample adding hole 21.
Be equipped with a transparent panel on observation window 22, play the effect of closed observation window 22 when guaranteeing to observe detection line T and quality control line C, can prevent that the foreign matter from getting into in the box body 2.
Specifically, the cover plate 3 may be a flip cover, a screw cover, or a sliding cover to open and close the sample adding hole 21, which is not limited in this embodiment.
As a specific implementation of this embodiment, a sliding groove 23 is provided on the box body 2, the cover plate 3 is slidably connected in the sliding groove 23, and the cover plate 3 slides in the sliding groove 23 to open and close the sample adding hole 21.
One end of the sliding groove 23 corresponds to the sample adding hole 21, when the sample adding hole 21 is closed, the cover plate 3 is positioned at one end of the sliding groove 23 corresponding to the sample adding hole 21, when the detection card is used, the cover plate 3 is slid to the other end of the sliding groove 23,
preferably, a concave part is arranged on the side edge of one end of the sliding chute 23 away from the sample adding hole 21, an elastic shifting piece 4 is arranged in the concave part, and the elastic shifting piece 4 extends obliquely towards the direction away from the sample adding hole 21; a concave cavity 31 matched with the elastic shifting piece 4 is arranged on the side wall of the cover plate 3;
the cover plate 3 slides, the elastic shifting sheet 4 is tightly propped against the side edge of the cover plate 3 to generate deformation, and the shape of the elastic shifting sheet 4 is recovered and extends into the concave cavity 31 after meeting the concave cavity 31, so that the elastic shifting sheet 4 fixes the cover plate 3;
when the cover plate 3 is positioned at one end of the sliding groove 23 corresponding to the sample adding hole 21, the elastic shifting piece 4 is in contact with the end part of the cover plate 3, and the elastic shifting piece 4 plays a role in limiting and fixing the cover plate 3, so that the cover plate 3 is ensured not to freely slide under the action of no external force;
the elastic shifting piece 4 is in a sheet shape and made of plastic, but in this embodiment, it is not excluded that the elastic shifting piece 4 is made of elastic material such as rubber.
More preferably, an anti-slip structure 32 is arranged on one side of the cover plate 3 away from the box body 2, so that friction is increased, and the cover plate 3 is conveniently pushed;
the anti-slip structure 32 may be an anti-slip pattern, an anti-slip bump, etc., which is not limited in this embodiment.
When using this detection card, need use the straw 5 to absorb the sample and drip it from application of sample hole 21 on sample pad 11, in this embodiment, one side of box body 2 is equipped with detachable straw 5, the convenience when improving the use.
The detachable connection of the suction pipe 5 can be realized by inserting the suction pipe 5 into a jack arranged on the box body 2, can also be realized by a magnetic suction structure and can also be realized by other detachable structures, and the embodiment does not limit the detachable connection.
As a specific implementation of this embodiment, an elastic snap ring 6 is disposed on one side of the box body 2, an opening for the straw 5 to enter is disposed on the elastic snap ring 6, the straw 5 is detachably connected to the elastic snap ring 6, and the straw 5 is clamped to the elastic snap ring 6;
the elastic clamping ring 6 can generate deformation after the suction pipe 5 is clamped and has a force of recovering the original shape, so that the suction pipe 5 is clamped;
the elastic snap ring 6 may be made of rubber, plastic, or the like, which is not limited in this embodiment.
More preferably, a limiting groove 7 for limiting the suction pipe 5 is further formed in one side, provided with the elastic clamping ring 6, of the box body 2, and the limiting groove 7 is provided with an opening part for the suction pipe 5 to enter;
When the detection card is used, the box body 2 needs to be inserted into a peripheral detection device, more preferably, the elastic snap ring 6 and the limiting groove 7 are both arranged on a mounting plate 8, the box body 2 is provided with a mounting groove 24 for mounting the mounting plate 8, one end of the mounting groove 24 is an open end for inserting the mounting plate 8, the mounting plate 8 and the mounting groove 24 are in abutting contact, the mounting plate 8 is ensured to be detachable, meanwhile, the mounting plate 8 is prevented from automatically sliding down without the action of external force, and the mounting stability is ensured;
when the box body 2 needs to be inserted into the peripheral equipment, the mounting plate 8 is detached from the mounting groove 24, and the box body 2 can be smoothly inserted into the clamping groove of the peripheral detection equipment.
While embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that: various changes, modifications, substitutions and alterations can be made to the embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the claims and their equivalents.
Claims (10)
1. A fluorescent microsphere lateral chromatography detection card for detecting a novel coronavirus antibody comprises a bottom plate (1) and is characterized in that a sample pad (11), a binding pad (12), a nitrocellulose membrane (13) and an absorption pad (14) are sequentially connected to the bottom plate (1) from left to right, and staphylococcal protein A (121) marked by fluorescent microspheres is loaded on the binding pad (12); the nitrocellulose membrane (13) is provided with a detection line (T) and a quality control line (C) in parallel, the detection line (T) is coated with a novel coronavirus N protein, and the quality control line (C) is coated with chicken IgY.
2. The fluorescent microsphere lateral chromatography detection card for detecting the novel coronavirus antibody according to claim 1, further comprising a box body (2), wherein the bottom plate (1) is positioned in the box body (2);
the box body (2) is also provided with a sample adding hole (21) corresponding to the sample pad (11) and an observation window (22) corresponding to the detection line (T) and the quality control line (C) on the nitrocellulose membrane (13).
3. The lateral chromatography detection card of fluorescent microspheres for detecting novel coronavirus antibody of claim 2, wherein the sample application hole (21) is provided with a cover plate (3) which can be opened and closed; the observation window (22) is provided with a transparent panel.
4. The lateral chromatography detection card of fluorescent microspheres for detecting novel coronavirus antibody of claim 3, wherein the box body (2) is provided with a sliding chute (23), the cover plate (3) is slidably connected in the sliding chute (23), and the cover plate (3) slides in the sliding chute (23) to open and close the sample adding hole (21).
5. The lateral chromatography detection card of fluorescent microspheres for detecting novel coronavirus antibodies of claim 4, wherein a concave part is arranged on the side of one end of the chute (23) far away from the sample adding hole (21), an elastic shifting sheet (4) is arranged in the concave part, and the elastic shifting sheet (4) extends obliquely towards the direction far away from the sample adding hole (21); a concave cavity (31) matched with the elastic shifting piece (4) is arranged on the side wall of the cover plate (3);
the cover plate (3) slides, the elastic shifting sheet (4) is tightly propped against the side edge of the cover plate (3) to generate deformation, and the shape of the elastic shifting sheet (4) is recovered and extends into the concave cavity (31) after encountering the concave cavity (31).
6. The lateral chromatography detection card of fluorescent microspheres for detecting novel coronavirus antibodies of claim 4, wherein the side of the cover plate (3) far away from the box body (2) is provided with an anti-slip structure (32).
7. The fluorescent microsphere lateral chromatography detection card for detecting the novel coronavirus antibody in claim 2, wherein a detachable pipette (5) is arranged on one side of the box body (2).
8. The fluorescent microsphere lateral chromatography detection card for detecting the novel coronavirus antibody as claimed in claim 7, wherein an elastic snap ring (6) is arranged on one side of the box body (2), an opening for the straw (5) to enter is arranged on the elastic snap ring (6), and the straw (5) is detachably connected to the elastic snap ring (6).
9. The fluorescent microsphere lateral chromatography detection card for detecting the novel coronavirus antibody according to claim 8, wherein one side of the box body (2) provided with the elastic snap ring (6) is further provided with a limiting groove (7) for limiting the straw (5), and the limiting groove (7) is provided with an open part for the straw (5) to enter;
the elastic clamping ring (6) and the limiting groove (7) are respectively arranged at two ends of the box body (2) in the length direction.
10. The lateral chromatography detection card of fluorescent microspheres for detecting novel coronavirus antibodies of claim 9, wherein the elastic snap ring (6) and the limiting groove (7) are both disposed on a mounting plate (8), the box body (2) is provided with a mounting groove (24) for mounting the mounting plate (8), one end of the mounting groove (24) is an open end for inserting the mounting plate (8), and the mounting plate (8) is detachably connected to the mounting groove (24).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120852295.0U CN214585479U (en) | 2021-04-23 | 2021-04-23 | Fluorescent microsphere lateral chromatography detection card for detecting novel coronavirus antibody |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120852295.0U CN214585479U (en) | 2021-04-23 | 2021-04-23 | Fluorescent microsphere lateral chromatography detection card for detecting novel coronavirus antibody |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214585479U true CN214585479U (en) | 2021-11-02 |
Family
ID=78327893
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202120852295.0U Active CN214585479U (en) | 2021-04-23 | 2021-04-23 | Fluorescent microsphere lateral chromatography detection card for detecting novel coronavirus antibody |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN214585479U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115032404A (en) * | 2022-06-10 | 2022-09-09 | 杨富松 | Vaccine antibody detection card, detection system and detection method thereof |
-
2021
- 2021-04-23 CN CN202120852295.0U patent/CN214585479U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115032404A (en) * | 2022-06-10 | 2022-09-09 | 杨富松 | Vaccine antibody detection card, detection system and detection method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2344544A1 (en) | Analytical test device and method of use | |
| CN205449995U (en) | Magnetic particle chemiluminescence micro -fluidic chip | |
| CN104714026A (en) | Separating detection composition of alpha-fetoprotein variant, system and application thereof | |
| CN214585479U (en) | Fluorescent microsphere lateral chromatography detection card for detecting novel coronavirus antibody | |
| CN101551397A (en) | Reagent box for detecting nature of third type hepatitis virus antibody by using chemiluminescence method | |
| CN104897907A (en) | Kit for detecting glycosylated hemoglobin and detection method thereof | |
| JP4157924B2 (en) | Glycated protein separation / detection device | |
| CN105572353A (en) | Antibody chip reagent kit for detecting hepatoma marker | |
| CN204422547U (en) | A kind of separation detection kit of alpha-fetoprotein variant and device | |
| CN109239326A (en) | Based on the micro-fluidic immuno-chip analysis method of magnetic particle nano enzyme and application | |
| CN206074619U (en) | One kind is occulted blood quick diagnosis chromatographic test paper | |
| CN206930677U (en) | A kind of cervical carcinoma quick detection kit using Eag1 potassium-channel proteins as target | |
| CN208856070U (en) | A kind of kit for alpha-fetoprotein detection | |
| CN202362304U (en) | Kit | |
| JP2005505755A5 (en) | ||
| CN220438354U (en) | Three-pad type detection test paper and detection kit thereof | |
| CN210427593U (en) | Kit for measuring hepatitis C virus NS3 antigen by magnetic bead chemiluminescence method | |
| CN215575181U (en) | Novel rapid detection card for coronavirus antibody | |
| CN215116313U (en) | Thyroid function joint detection kit | |
| CN220893921U (en) | Kit for immunohistochemical staining | |
| CN212111448U (en) | Detection reagent strip and kit for rapidly determining microalbuminuria | |
| WO1999053288A2 (en) | Systems and methods for interpreting immunoblot test results | |
| CN211627586U (en) | Human B cell maturation antigen detection kit | |
| CN214201199U (en) | Chemiluminescence detection reagent strip | |
| CN205484360U (en) | Test paper area of sxemiquantitative short -term test rabies viruses antibody |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |