CN215116313U - Thyroid function joint detection kit - Google Patents
Thyroid function joint detection kit Download PDFInfo
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- CN215116313U CN215116313U CN202121102031.XU CN202121102031U CN215116313U CN 215116313 U CN215116313 U CN 215116313U CN 202121102031 U CN202121102031 U CN 202121102031U CN 215116313 U CN215116313 U CN 215116313U
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Abstract
The utility model discloses a thyroid function joint detection kit, relating to the technical field of biological monitoring; the kit comprises an integrated detection test strip capable of realizing two tests simultaneously, and comprises a support plate, a glass cellulose membrane, a nitrocellulose membrane, a water absorption pad and a sample pad, wherein the support plate is of a long strip structure and is positioned at the bottommost part, the water absorption pad is arranged at the middle position above the support plate, and then the two ends of the water absorption pad are sequentially provided with the nitrocellulose membrane, the glass cellulose membrane and the sample pad respectively. The detection kit is used for simultaneously detecting the detection results of the five thyroid function markers, has no dependence on any experimental instrument, environment or operator, and is simple and convenient to operate, short in detection period and easy to interpret; in addition, special instruments and equipment are not needed, professional training is not needed, the adaptability is strong, and the monitoring and the inspection at any time and any place are convenient.
Description
Technical Field
The utility model relates to a biological detection field, concretely relates to thyroid function joint detection kit.
Background
Thyroid Stimulating Hormone (TSH) is a hormone secreted by the pituitary gland, which secretes thyroid stimulating hormone, which is on the one hand, influenced by the stimulation of thyroid stimulating hormone releasing hormone (TRH) secreted by the hypothalamus and on the other hand, influenced by the inhibition of the feedback of thyroid hormone, which are antagonistic to each other, and which constitute the hypothalamic-adenohypophysis-thyroid axis. Thyroid stimulating hormone is mainly responsible for regulating the proliferation of thyroid cells, thyroid blood supply, and synthesis and secretion of thyroid hormone, and plays the most important regulatory role in maintaining normal thyroid function. Diseases of the pituitary itself can directly affect the synthesis and release of TSH. Secretion of pituitary TSH and serum TSH levels can also be affected when thyroid gland itself causes abnormalities in thyroid hormone synthesis and secretion. Similarly, hypothalamic disease affects TRH secretion and affects pituitary TSH secretion and serum TSH levels. TSH is one of the hormones secreted by the anterior pituitary, and its main function is to control and regulate the activity of the thyroid gland. The measurement of thyroid stimulating hormone in serum (plasma) is one of the important indicators for the diagnosis and treatment of hyperthyroidism and hypothyroidism and for the study of the hypothalamic-pituitary-thyroid axis. The kit is an indispensable tool in diagnosing thyroid hypofunction, and differentially diagnosing primary and secondary (hypothalamic or prolapsing) hypothyroidism and the like. TSH can be used as index for determining therapeutic effect in treating hyperthyroidism and hypothyroidism. In addition, it can be used to observe the storage function of pituitary TSH, and further distinguish between hypothalamic and pituitary lesions. TSH testing is a preliminary screening test to ascertain thyroid function. Small changes in free thyroid concentration will result in significant adjustment of TSH concentration in the opposite direction.
1. Serum TSH elevation: it is commonly seen in primary hypothyroidism, TSH secretory tumor, iodine deficiency endemic goiter, thyroid hormone resistance syndrome, etc.
2. Serum TSH reduction: it is commonly seen in primary hyperthyroidism, TSH gene mutation, various pituitary diseases (such as pituitary adenoma, pituitary inflammation, pituitary hemorrhagic disease or traumatic disease) affecting TSH cell function, various damage stages of thyroiditis, and clinical application of large dose of glucocorticoid
T3 also known as triiodothyronine
Thyroxine (T4) also known as tetraiodothyronine
They are a group of iodized tyrosines, which are synthesized in thyroid gland cells by using iodine and tyrosine as raw materials.
When thyroid gland is acted by TSH to release thyroid hormone, glandular epithelial cells firstly swallow thyroglobulin in follicular cavity into glandular cells by means of swallowing action, under the action of lysosome proteolytic enzyme the thyroglobulin is decomposed, and the released T4 and T3 have small molecules and can be passed through capillary to make blood circulation. The amount of T4 on the thyroglobulin molecule is far more than that of T3, so that the secreted hormone contains about 90% of T4, and T3 has a smaller secretion amount but 5 times as high activity as T4. The total amount of T4 secreted per day was about 96. mu.g, and T3 was about 30. mu.g. After T4 is released into blood, one part is combined with plasma protein, the other part is transported in blood in free state, the two parts can be mutually converted, and dynamic balance of T4 and T3 in blood is maintained, because only free type can enter cells to play a role. T3 is mainly present in free form after release into the blood because of its low affinity for plasma proteins. About 50% of the daily deiodination of T4 is converted to T3, so the effect of T3 is not negligible.
Thus, there is a need for a method for diagnosing thyroid function in an early, rapid, simple and reliable manner.
SUMMERY OF THE UTILITY MODEL
In order to solve the technical problem, the utility model provides a thyroid function joint detection kit, its concrete scheme is as follows:
the utility model provides a thyroid function joint detection kit, including an integration test paper strip that can realize two surveys simultaneously in the thyroid function joint detection kit, including backup pad, glass cellulose membrane, nitrocellulose membrane, the pad that absorbs water and sample pad, wherein, the backup pad is rectangular structure, is located the bottommost the intermediate position of backup pad top is provided with the pad that absorbs water, then the pad both ends that absorb water have set gradually respectively nitrocellulose membrane glass cellulose membrane with the sample pad.
Preferably, the top end of the sample pad presses and adheres to the bottom end of the glass cellulose membrane, the top end of the glass cellulose membrane presses and adheres to the bottom end of the nitrocellulose membrane, and the top end of the nitrocellulose membrane is pressed and adheres to the bottom end of the water-absorbing filter paper.
Preferably, the integrated detection test strip is divided into a first test strip and a second test strip which share a water absorption pad, wherein three detection lines and one quality control line are arranged on the nitrocellulose membrane on the first test strip, two detection lines and one quality control line are arranged on the nitrocellulose membrane on the second test strip, the detection lines are sequentially arranged on one side close to the glass cellulose membrane, and the quality control strip is arranged on one side close to the water absorption pad.
Preferably, the three detection lines and the quality control line of the first test strip are all spaced by 6-8mm, preferably 7mm, and the three detection lines respectively comprise a Thyroid Stimulating Hormone (TSH) antibody, a triiodothyronine (T3) -BSA antigen and a thyroxine (T4) -BSA antigen; the quality control line comprises a goat anti-chicken IgY polyclonal antibody.
Preferably, the two detection lines and the quality control line of the second test strip are separated by 9-10 mm; the two detection lines respectively comprise a T3-BSA antigen and a T4-BSA antigen; the quality control line comprises a goat anti-chicken IgY polyclonal antibody.
Preferably, the length of the water absorption pad is 40-45mm, and the overlapping length of the water absorption pad and the nitrocellulose membrane which are overlapped and stuck together is 1 mm;
the length of the glass cellulose membrane is 6-8mm, preferably 7mm, and the overlapping length of the overlapping and sticking the glass cellulose membrane box and the nitrocellulose membrane together is 1-2mm, preferably 1.5 mm.
The length of the sample pad is 25-27mm, preferably 26mm, and the overlapping length of the sample pad and the glass cellulose membrane which are overlapped and stuck together is 1-2mm, preferably 1.5 mm.
Preferably, the glass cellulose membrane contains a TSH \ T3\ T4 antibody which is quantum dot labeled by adopting a sodium carboxymethyl cellulose system.
Preferably, the support plate is a plastic plate.
Preferably, thyroid function joint detection kit still includes a test paper strip shell, the integration test paper strip is installed the test paper strip shell, be provided with application of sample mouth and visual window on the shell, the application of sample mouth is located the sample pad at integration test paper strip both ends is the application of sample mouth A on the first test paper strip and the application of sample mouth B on the second test paper strip respectively, visual window is located directly over the nitrocellulose of integration test paper strip.
The method for detecting by adopting the thyroid function joint detection kit comprises the following steps:
(1) pretreating a sample to be detected by using a diluent and a dissociation solution respectively;
(2) dripping the sample treated by the diluent on a sample application port A of the detection kit; dropwise adding the sample treated by the dissociation solution to a sample application port B of the detection kit; the sample moves towards the water absorption pad along the glass cellulose membrane and the nitrocellulose membrane on the support plate simultaneously;
(3) if the sample contains thyroid function markers, triiodothyronine, thyroxine, free triiodothyronine, free thyroxine and serum thyrotropin in the markers are captured by a mixture of triiodothyronine, thyroxine, free triiodothyronine, free thyroxine and serum thyrotropin antibodies marked in the glass cellulose membrane to form antigen-antibody conjugates, and then the antigen-antibody conjugates are specifically bound with antigens on each detection line when passing through the nitrocellulose membrane, so that a fluorescence effect is generated; the TSH fluorescence is increased and decreased along with the increase of the concentration of the marker in the sample, TT3, TT4, FT3 and FT4 are decreased along with the increase of the concentration of the corresponding marker, and the fluorescence is increased along with the decrease of the concentration of the marker.
The utility model has the advantages as follows:
the utility model provides a thyroid function joint detection kit can simplify the detection flow of thyroid function in the blood sample. The detection kit is used for simultaneously detecting the detection results of the five thyroid function markers, has no dependence on any experimental instrument, environment or operator, and is simple and convenient to operate, short in detection period and easy to interpret; in addition, special instruments and equipment are not needed, professional training is not needed, the adaptability is strong, and the monitoring and the inspection at any time and any place are convenient.
The utility model provides a detection method easy operation is convenient, do not rely on higher laboratory condition, be used for short-term test thyroid gland function.
Drawings
Fig. 1 is a schematic structural diagram of a test paper strip in a thyroid function joint detection kit provided by the present invention;
FIG. 2 is a top view of the casing structure of the thyroid function joint detection kit provided in the present invention;
wherein: 1. a sample end; 2. a support plate; 3. a glass cellulose membrane; 4. a nitrocellulose membrane; 5. a water absorption end; 6. a TSH antibody detection line; 7 is the FT3 detection line; 8 is the FT4 test line; 9 is a quality control line, 10 is a TT3 detection line, 11 is a TT4 detection line, 12 is a first test strip visual window area, 13 is a second test strip visual window area, 14 is a sample application port A, and 15 is a sample application port B.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings. It should be understood that the detailed description and specific examples, while indicating the invention, are given by way of illustration only.
Example 1
This embodiment provides a thyroid function joint detection kit, including an integration test paper strip and the test paper strip shell that can realize two surveys simultaneously in the thyroid function joint detection kit, wherein integration test paper strip is installed inside the test paper strip shell, integration test paper strip includes backup pad, glass cellulose membrane, nitrocellulose membrane, water absorption pad and sample pad, wherein, the backup pad is rectangular structure, is located the bottommost intermediate position of backup pad top is provided with the water absorption pad, then water absorption pad both ends have set gradually respectively nitrocellulose membrane the glass cellulose membrane with the sample pad, as shown in fig. 1. Be provided with application of sample mouth and visual window on the shell, as shown in figure 2, the application of sample mouth is located the sample pad at integration test paper strip both ends is the application of sample mouth A on the first test paper strip and the application of sample mouth B on the second test paper strip respectively, the visual window is located directly over the nitrocellulose of integration test paper strip, be respectively first test paper strip visual window region and second test paper strip visual window region.
In this embodiment, the top end of the sample pad presses and adheres to the bottom end of the glass cellulose membrane, the top end of the glass cellulose membrane presses and adheres to the bottom end of the nitrocellulose membrane, and the top end of the nitrocellulose membrane is pressed and adheres to the bottom end of the water-absorbent filter paper. The length of the water absorption pad is 40-45mm, and the overlapping length of the water absorption pad and the nitrocellulose membrane which are overlapped and stuck together is 1 mm; the length of the glass cellulose membrane is 6-8mm, most preferably 7mm, and the overlapping length of the overlapping and sticking the glass cellulose membrane box and the nitrocellulose membrane together is 1-2mm, most preferably 1.5 mm. The length of the sample pad is 25-27mm, most preferably 26mm, and the overlapping length of the sample pad and the glass cellulose membrane which are overlapped and stuck together is 1-2mm, preferably 1.5 mm.
In this embodiment, three detection lines and one quality control line are disposed on the nitrocellulose membrane of the first test strip, two detection lines and one quality control line are disposed on the nitrocellulose membrane of the second test strip, the detection lines are sequentially disposed on a side close to the glass cellulose membrane, and the quality control strip is disposed on a side close to the absorbent pad.
In this embodiment, the three detection lines and the quality control line of the first test strip are all spaced by 6-8mm, preferably 7mm, and each of the three detection lines respectively comprises a Thyroid Stimulating Hormone (TSH) antibody, triiodothyronine (T3) -BSA antigen, and thyroxine (T4) -BSA antigen; the quality control line comprises a goat anti-chicken IgY polyclonal antibody.
In the embodiment, the two detection lines and the quality control line of the second test strip are separated by 9-10 mm; the two detection lines respectively comprise a T3-BSA antigen and a T4-BSA antigen; the quality control line comprises a goat anti-chicken IgY polyclonal antibody.
The glass cellulose membrane in the embodiment contains a TSH \ T3\ T4 antibody which is quantum dot labeled by adopting a sodium carboxymethylcellulose system.
Preferably, the support plate is a plastic plate.
The utility model provides a method for detecting by applying thyroid function joint detection kit, comprising the following steps:
(1) pretreating a sample to be detected by using a diluent and a dissociation solution respectively;
(2) dripping the sample treated by the diluent on a sample application port A of the detection kit; dropwise adding the sample treated by the dissociation solution to a sample application port B of the detection kit; the sample moves towards the water absorption pad along the glass cellulose membrane and the nitrocellulose membrane on the support plate simultaneously;
(3) if the sample contains thyroid function markers, triiodothyronine, thyroxine, free triiodothyronine, free thyroxine and serum thyrotropin in the markers are captured by a mixture of triiodothyronine, thyroxine, free triiodothyronine, free thyroxine and serum thyrotropin antibodies marked in the glass cellulose membrane to form antigen-antibody conjugates, and then the antigen-antibody conjugates are specifically bound with antigens on each detection line when passing through the nitrocellulose membrane, so that a fluorescence effect is generated; the TSH fluorescence is increased and decreased along with the increase of the concentration of the marker in the sample, TT3, TT4, FT3 and FT4 are decreased along with the increase of the concentration of the corresponding marker, and the fluorescence is increased along with the decrease of the concentration of the marker.
Through adopting the utility model discloses an above-mentioned technical scheme has obtained following profitable effect:
the utility model provides a thyroid function joint detection kit can simplify the detection flow of thyroid function in the blood sample. The detection kit is used for detecting the thyroid function, has no dependence on any experimental instrument, environment or operator, and has the advantages of simple and convenient operation, short detection period and easy interpretation; in addition, special instruments and equipment are not needed, professional training is not needed, the adaptability is strong, and the monitoring and the inspection at any time and any place are convenient.
The utility model provides a detection method easy operation is convenient, do not rely on higher laboratory condition, be used for short-term test thyroid gland function.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of improvements and decorations can be made without departing from the principle of the present invention, and these improvements and decorations should also be viewed as the protection scope of the present invention.
Claims (7)
1. A thyroid function joint detection kit is characterized in that the thyroid function joint detection kit comprises an integrated detection test strip capable of realizing two detections, and the integrated detection test strip comprises a support plate, a glass cellulose membrane, a nitrocellulose membrane, a water absorption pad and a sample pad, wherein the support plate is of a long strip structure and is positioned at the bottommost part, the water absorption pad is arranged at the middle position above the support plate, and then the nitrocellulose membrane, the glass cellulose membrane and the sample pad are sequentially arranged at two ends of the water absorption pad;
the integrated detection test strip is divided into a first test strip and a second test strip which share a water absorption pad, wherein the first test strip and the second test strip share the water absorption pad
The detection line is sequentially arranged on one side close to the glass cellulose membrane, and the quality control line is arranged on one side close to the water absorption pad.
2. The kit for the combined detection of thyroid function according to claim 1, wherein the top end of the sample pad is pressed against and adhered to the bottom end of the glass cellulose membrane, the top end of the glass cellulose membrane is pressed against and adhered to the bottom end of the nitrocellulose membrane, and the top end of the nitrocellulose membrane is pressed against and adhered to the bottom end of the absorbent pad.
3. The thyroid function joint detection kit according to claim 1, wherein the three detection lines and the quality control line of the first test strip are all spaced by 6-8mm, and each of the three detection lines comprises a Thyroid Stimulating Hormone (TSH) antibody, triiodothyronine (T3) -BSA antigen and thyroxine (T4) -BSA antigen; the quality control line comprises a goat anti-chicken IgY polyclonal antibody.
4. The thyroid function joint detection kit according to claim 1, wherein the two detection lines and the quality control line of the second test strip are separated by 9-10 mm; the two detection lines respectively comprise a T3-BSA antigen and a T4-BSA antigen; the quality control line comprises a goat anti-chicken IgY polyclonal antibody.
5. The thyroid function joint detection kit according to claim 1, wherein the absorbent pad has a length of 40-45mm, and the absorbent pad and the nitrocellulose membrane are stuck together in an overlapping manner and have an overlapping length of 1 mm;
the length of the glass cellulose membrane is 6-8mm, and the overlapping length of the overlapping and sticking of the glass cellulose membrane box and the nitrocellulose membrane is 1-2 mm;
the length of the sample pad is 25-27mm, and the overlapping length of the sample pad and the glass cellulose membrane which are overlapped and stuck together is 1-2 mm.
6. The kit for the combined detection of thyroid function according to claim 1, wherein the support plate is a plastic plate.
7. The kit for thyroid function combination detection according to claim 1, further comprising a test strip housing, wherein the integrated test strip is mounted on the test strip housing, the housing is provided with a sample application port and a visual window, the sample application port is located on the sample pad at two ends of the integrated test strip, and the visual window is located directly above the nitrocellulose of the integrated test strip.
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| CN202121102031.XU CN215116313U (en) | 2021-05-21 | 2021-05-21 | Thyroid function joint detection kit |
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| CN202121102031.XU CN215116313U (en) | 2021-05-21 | 2021-05-21 | Thyroid function joint detection kit |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN118050505A (en) * | 2024-04-15 | 2024-05-17 | 南京佰抗生物科技有限公司 | Multiple combined inspection fluorescence immunochromatography detection card |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118050505A (en) * | 2024-04-15 | 2024-05-17 | 南京佰抗生物科技有限公司 | Multiple combined inspection fluorescence immunochromatography detection card |
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Address after: 102629 No.25 Yongxing Road, Daxing biomedical industrial base, Zhongguancun Science Park, Daxing District, Beijing Patentee after: Beijing Easysweet Biotechnology Co.,Ltd. Patentee after: Fanjing Biotechnology (Shandong) Co.,Ltd. Address before: 102629 No.25 Yongxing Road, Daxing biomedical industrial base, Zhongguancun Science Park, Daxing District, Beijing Patentee before: Beijing Easysweet Biotechnology Co.,Ltd. Patentee before: Xintai Fanjing Biotechnology Co.,Ltd. |
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Effective date of registration: 20220909 Address after: No. 1111, Fuqian Street, Qingyun Street, Xintai City, Tai'an City, Shandong Province, 271000 Patentee after: Fanjing Biotechnology (Shandong) Co.,Ltd. Address before: 102629 No.25 Yongxing Road, Daxing biomedical industrial base, Zhongguancun Science Park, Daxing District, Beijing Patentee before: Beijing Easysweet Biotechnology Co.,Ltd. Patentee before: Fanjing Biotechnology (Shandong) Co.,Ltd. |
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