CN206638678U - Multi objective time-resolved fluoroimmunoassay for postoperative patient monitoring chromatographs kit - Google Patents
Multi objective time-resolved fluoroimmunoassay for postoperative patient monitoring chromatographs kit Download PDFInfo
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- CN206638678U CN206638678U CN201720450273.5U CN201720450273U CN206638678U CN 206638678 U CN206638678 U CN 206638678U CN 201720450273 U CN201720450273 U CN 201720450273U CN 206638678 U CN206638678 U CN 206638678U
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Abstract
It the utility model is related to a kind of multi objective time-resolved fluoroimmunoassay chromatography kit for postoperative patient monitoring, including detection card and selectable fluorescence immunoassay quantitative analysis instrument, detection card includes loading pad, pad, detecting pad with detection line and nature controlling line, sample suction pad and bottom plate, the index includes Procalcitonin, the gelatinase-associated lipocalin protein of neutrophil leucocyte and N-terminal brain natriuretic peptide, the pad is included with reference to pad body and coated on the time-resolved fluorescence microballoon labelled antibody coating combined on pad body, antibody in the time-resolved fluorescence microballoon labelled antibody coating is the antibody of the index；The risk of inflammation, renal failure and heart failure after openheart surgery is monitored using tri- kinds of PCT, NGAL, NT proBNP labels, while can be effectively, the kit diagnostic sensitivity is high, high specificity, and the accuracy rate of clinical monitoring is up to more than 95%.
Description
Technical field
The utility model is related to biotechnology diagnostic field, and in particular to it is a kind of for postoperative patient monitoring multi objective when
Between resolved fluorometric immune chromatography reagent kit.
Background technology
Patient's postoperative complication is more, and the lighter has heating, increased heart rate, shortness of breath, poor appetite, nausea, leucocyte to increase
Height, severe one may occur in which that low blood pressure, expiratory dyspnea, oliguresis, stupor are even dead.Operation wound, ooze out increase, foreign matter in heart
The factor such as implantation, antibiotic misapplication, Post operation immunity degradation, is easily caused operative site or the infection at other positions.
Acute renal failure is a severe complication of after cardiac surgery, up to 30%, once acute renal failure occurs, suffer from
Person's postoperative death rate drastically raises, up to 60%.Especially postoperative low blood pressure, the time, which has been grown, is easy to that acute renal failure occurs, just
Phase shows as oliguresis, anuria, and serum creatinine, urea nitrogen are gradually increasing therewith, and it is overweight potassemia, Water l oad occur, such as not in time
Processing, often makes the further exhaustion of heart function, pulmonary edema occurs, the state of an illness is very dangerous.
Procalcitonin (Procalcitonin, PCT) be calcitonin C T precursor substance, be one kind without hormonal activity sugar egg
In vain, be made up of 116 amino acid, molecular weight 13kD, be made up of N-terminal-calcitonin-C-terminal three parts, its Stability Analysis of Structures not by
The influence of hormone in vivo level.PCT is as a kind of clinical infection mark, in systemic bacterial infections patient and sepsis patient
Content raises rapidly in serum, directly proportional to the order of severity of inflammation, in system inflammation response syndrome, pyemia, serious purulence
PCT concentration has notable difference in toxication and septic shock patient.PCT can be detected after 2 hours in infection, 12- after infection
Peak within 24 hours, do not influenceed by clinical application, for systemic bacterial infection and pyemia etc. have high susceptibility and
Specificity.Clinical research shows that PCT detections have very high value in diagnosis of the different medical domains to a variety of diseases and treatment,
It is the important symbol thing for distinguishing bacterium and non-bacterial infection.Research points out that complication patient's art occurs for after cardiac surgery early stage
24h, 48h, 72h PCT concentration are higher than postoperative no complication patient afterwards.PCT is that a kind of early stage, Sensitivity and Specificity are splendid
High-risk monitoring index；Measure PCT contributes to the clinical high-risk patient for screening after cardiac surgery infection.
The gelatinase-associated lipocalin protein of neutrophil leucocyte (NGAL) is a kind of small-molecular-weight secreted protein, by
Kjeldsedn et al. had found in 1993, was the newcomer of Lipocalin families.Under physiological condition, NCAL synthesizes in bone on a small quantity
The epithelial cell of multiple systems such as marrow neutrophil leucocyte and renal tubule, inflammatory reaction and malignant tumour can lure that it is being organized more into
Largely generated in (such as uterus, prostate, salivary gland, lung, kidney, air flue and alimentary canal epithelium), can reaches in several hours
To peak value, produce speed and be significantly faster than that other relevant biomarkers things.NGAL has just expressed in acute tubular damage early stage,
Reflect that renal tubular function is disorderly, renal damage is prompted in the rise of its concentration, and the rise of traditional index serum creatinine then needs 24
More than hour.The child study that acute injury of kidney occurs postoperative to 20 Shunts such as Mishra is found, is made when with 50ug/l
During to diagnose cut off value, the Sensitivity and Specificity of urine NGAL detection acute injury of kidney is respectively 100% and 98%, and subject works
Feature（ROC）TG-AUC is 99.8%.Dent etc. to children's blood plasma NGAL of 120 customary cardiopulmonary bypass surgeries after monitoring
It was found that if acute injury of kidney occurs, 2h can raise 3 times to blood NGAL after surgery, and serum creatinine raises for 2-3 days after surgery.
NGAL is the biomarker of current early stage AKI diagnosis.
NT-proBNP（N-terminal brain natriuretic peptide）, it is the product after proBNP (proBNP) is cut by endopeptidase, containing N-terminal 76
Individual amino acid.When heart failure occurs, BNP is discharged into blood, is then degraded to NT-proBNP and BNP, is detected in blood
NT-proBNP and BNP content, the degree of heart failure can be monitored.Because NT-proBNP half-life period is longer (120min),
Vitro stability is strong, and the concentration in heart failure patient is high compared with BNP, is more beneficial for the diagnosis of heart failure, therefore NT-
Sensitive indicators of the proBNP as diagnosing patients with heart failure, and the evaluation of cardiac function infringement order of severity.U.S. FDA approval in 2002
The ElecsysproBNP listings of Roche companies, detect the NT-proBNP in blood.American Heart Association (AHA), U.S. clinical
In " kit for diagnosing heart failure and treatment guidelines " and " application guide of cardiac marker " that biochemical institute (NACP) is formulated, NT-
Diagnosis and prognostic indicator of the proBNP as heart failure.
Detection PCT, NGAL or NT-proBNP diagnostic products are clinically applied in different forms at present, are made
Principle and operation sequence are different, and compatibility is poor, it is difficult in a short time simultaneously, be quickly detected from the knot of three
Fruit, the timely diagnosis to the cardiorenal function of patient and infection is greatly affected, so as to causing many patients because of delay treatment
Sb.'s illness took a turn for the worse even results in death.The utility model kit can realize inflammation, heart failure, the synchronization of renal failure, fast and accurately
Detection.
In addition, it is used for the detection cartoon of the kit of postoperative patient monitoring in the prior art often by loading pad, pad, tool
It is made up of the detecting pad and sample suction pad of detection line and nature controlling line, this kind detection card is adapted only to blood serum sample, and blood plasma and whole blood
Product can not detect, it is necessary to can be detected after centrifugal treating, the limitations affect convenience of instant detection.
The content of the invention
Technical problem to be solved in the utility model is to provide a kind of multi objective time point for postoperative patient monitoring
Fluorescence immune chromatography kit is distinguished, can Quantitative detection calcium element be former simultaneously, the gelatinase-associated lipocalin of neutrophil leucocyte
Three indexs of albumen and N-terminal brain natriuretic peptide.
To solve above technical problem, a kind of multi objective time point for postoperative patient monitoring provided by the utility model
Distinguish that fluorescence immune chromatography kit protocol is as follows：Kit includes detection card and selectable fluorescence immunoassay quantitative analysis instrument, inspection
Surveying card includes loading pad, pad, detecting pad, sample suction pad and the bottom plate with detection line and nature controlling line, and Testing index includes drop
Calcium element is former（PCT）, the gelatinase-associated lipocalin protein of neutrophil leucocyte（NGAL）With N-terminal brain natriuretic peptide（NT-proBNP）,
Pad is included with reference to pad body and coated on the time-resolved fluorescence microballoon labelled antibody coating combined on pad body, the time
Antibody in resolved fluorometric microballoon labelled antibody coating is the antibody of the index；Detection line has three, this three detections line phase
Mutually separation and the pairing antibody or antigen that can respectively be specifically bound with time-resolved fluorescence microballoon labelled antibody are formed
Coating.
According to one side of the present utility model, it is coated with detecting pad corresponding with PCT, NGAL, NT-proBNP respectively
Monoclonal antibody, polyclonal antibody, or corresponding antigen form detection line and sheep anti-mouse igg, goat-anti chicken IgY or goat-anti rabbit
The nature controlling line that the antibody such as IgG are formed.
The utility model also provides a kind of multi objective time-resolved fluoroimmunoassay chromatography reagent for postoperative patient monitoring
The scheme of box, the kit include detection card and selectable fluorescence immunoassay quantitative analysis instrument, and detection card includes loading pad, combined
Pad, detecting pad, sample suction pad and the bottom plate with detection line and nature controlling line, index include Procalcitonin, neutrophil leucocyte gelatinase
Correlation lipocalin protein and N-terminal brain natriuretic peptide, kit also include fluorescent labeled antibody solution, wherein time-resolved fluorescence
Antibody in microballoon labelled antibody coating is the antibody of the index；Detection line has three, this three detections line be separated from each other and
The coating that the pairing antibody or antigen that can be respectively specifically bound with time-resolved fluorescence microballoon labelled antibody are formed.
According to a specific aspect of the present utility model, detection blocks by loading pad, pad, detecting pad and sample suction pad successively
Interlaced be pasted onto on bottom plate is formed.
According to a preferred aspect of the present utility model, detection card also includes hemofiltration film, and hemofiltration film is covered on loading pad,
Loading pad, pad, detecting pad and sample suction pad are interlaced successively to be pasted onto on bottom plate.
According to another preferred aspect of the present utility model, detection card also includes boosting pad, and detects card by loading pad, combination
Interlaced be pasted onto on bottom plate is formed successively for pad, boosting pad, detecting pad and sample suction pad.
According to an also preferred aspect of the present utility model, detection card also includes hemofiltration film and boosting pad, and hemofiltration film is covered in
On loading pad, loading pad, pad, boosting pad, detecting pad and sample suction pad is interlaced successively is pasted onto on bottom plate.
Preferably, the material of boosting pad is glass fibre element film or non-woven fabrics.
According to a preferred aspect of the present utility model, the detecting pad only has one, the three detections line, nature controlling line
It is parallel to each other and is successively set on along the length direction of the detecting pad on the detecting pad.
Further, the detection line in three detections line close to pad applies for the pairing antibody or antigen of Procalcitonin
Layer, the detection line close to sample suction pad are the pairing antibody or antigen coating of N-terminal brain natriuretic peptide, and middle detection line is that neutral grain is thin
The pairing antibody or antigen coating of the gelatinase-associated lipocalin protein of born of the same parents, nature controlling line are located at the close sample suction pad of detecting pad
End.
According to another preferred aspect of the present utility model, the detecting pad includes three detection point pads, three detections
Line is respectively distributed on three detection point pads.Nature controlling line is respectively arranged with each detection point pad.PCT、NGAL、NT-proBNP
Corresponding monoclonal antibody, polyclonal antibody, or corresponding antigen are coated on three detecting pads respectively, on every detecting pad
Be arranged in parallel nature controlling line, and coating includes but is not limited to sheep anti-mouse igg, goat-anti chicken IgY or goat anti-rabbit igg antibody, or only to combining
Pad is handled by glass treatment fluid, and it is mono- to prepare the PCT monoclonal antibodies comprising fluorescent microsphere mark, the NGAL of fluorescent microsphere mark
The fluorescent liquid of the NT-proBNP monoclonal antibodies of clonal antibody and fluorescent microsphere mark.
According to another preferred aspect of the present utility model, detection card includes three independent detections point card, loading pad, combines
Pad, sample suction pad and bottom plate have three respectively, and each loading pad, pad, detection point pad, sample suction pad and bottom plate are assembled into one
Detection point card.
Preferably, nature controlling line includes but is not limited to the shapes such as sheep anti-mouse igg, goat-anti chicken IgY or goat anti-rabbit igg by coating
Into.
Preferably, detecting pad is nitrocellulose filter and is 5~12um of aperture porous spline structure film；Loading pad, combine
The material of pad is glass fibre element film or non-woven fabrics, and the material of sample suction pad is absorbent filter.
Fluorescent microsphere TIME RESOLVED TECHNIQUE and immuno-chromatographic assay technology are known.Time described in the utility model point
Distinguish that fluorescent microsphere labelled antibody can use method well known in the art to prepare.
Due to the implementation of above technical scheme, the utility model has the following advantages that compared with prior art：
The utility model kit includes quick detection inflammation, card is surveyed in renal failure and three joint inspections of heart failure, its use PCT,
Tri- kinds of labels of NGAL, NT-proBNP, the risk of inflammation, renal failure and heart failure after openheart surgery is monitored while can be effectively.The examination
Agent box diagnostic sensitivity is high, high specificity, and the accuracy rate of clinical monitoring is up to more than 95%, make postoperative patient inflammation, renal failure and
The at-once monitor of heart failure is possibly realized, and preventing patient in time, sb.'s illness took a turn for the worse.
Further, the utility model kit is by increasing hemofiltration film, suitable for whole blood test, after being easy to patient discharge
Cardiorenal function is carried out in Community health service and infection detects, and mitigation goes to hospital to register, is lined up, chemically examining, taking the cumbersome mistakes such as report
Journey, mitigate the pressure of public medical mechanism, be relatively beneficial to the daily monitoring of patient.
Further, the utility model adds boosting pad in structure, can effectively remove ambient interferences, so as to enter
One step improves diagnostic sensitivity height and the degree of accuracy, and boosting pad additionally aids quickening detection sample flow in addition, improves detection efficiency,
Shorten detection time.
Brief description of the drawings
Fig. 1 is the main structure diagram blocked according to the detection of embodiment 1 of the present utility model；
Fig. 2 is the main structure diagram blocked according to the detection of embodiment 2 of the present utility model；
Fig. 3 is the dimensional structure diagram blocked according to the detection of embodiment 3 of the present utility model；
Fig. 4 is the main structure diagram according to the detection point card of embodiment 4 of the present utility model（Detection line containing T1）；
Fig. 5 is the main structure diagram according to the detection point card of embodiment 4 of the present utility model（Detection line containing T2）；
Fig. 6 is the main structure diagram according to the detection point card of embodiment 4 of the present utility model（Detection line containing T3）；
Wherein：1,1 ', 1 ", loading pad；2,2 ', 2 ", gold conjugation pad；20,20 ', 20 ", with reference to pad body；21,
21 ', 21 ", colloidal gold labeled monoclonal antibody coating；3rd, detecting pad；30,30 ', 30 ", detection point pad；4,4 ', 4 ", sample suction pad；5,5’,
5 ", bottom plate；6,6 ', 6 ", hemofiltration film；7,7 ', 7 ", boosting pad；T1, T2, T3, detection line；C, C ', C ", nature controlling line.
Embodiment
Embodiment 1
As shown in figure 1, the multi objective time-resolved fluoroimmunoassay for postoperative patient monitoring chromatographs kit, it includes inspection
Card, fluorescence immunoassay quantitative analysis instrument are surveyed, detection card includes loading pad 1, pad 2, has detection line and nature controlling line C detecting pad
3rd, sample suction pad 4 and bottom plate 5, hemofiltration film 6 and boosting pad 7, hemofiltration film 6 are covered on loading pad 1, loading pad 1, pad 2, detection
Pad 3 and sample suction pad 4 is interlaced successively is pasted onto on bottom plate 5.Wherein, the material of boosting pad 7 is glass fibre element film or nonwoven
Cloth.Detecting pad 3 is nitrocellulose filter and is 5~12um of aperture porous spline structure film；Loading pad 1, the material of pad 2 are
Glass fibre element film or non-woven fabrics, the material of sample suction pad 4 is absorbent filter.
Further, detection card can detect the gelatinase-associated lipocalin egg of Procalcitonin, neutrophil leucocyte simultaneously
White and three kinds of indexs of N-terminal brain natriuretic peptide, pad 2 are included with reference to pad body 20 and coated on the time point combined on pad body 20
Fluorescent microsphere labelled antibody coating 21 is distinguished, the antibody in the time-resolved fluorescence microballoon labelled antibody coating 21 is the index
Antibody.The detecting pad 3 be one, have three detections line T1, T2, T3 thereon, this three detections line T1, T2, T3 be separated from each other and
The coating that the pairing antibody or antigen of respectively three kinds of marks are formed, the pairing antibody or antigen of three kinds of marks are able to
Specifically bound with time-resolved fluorescence microballoon labelled antibody coating.Three detections line T1, T2, T3, nature controlling line C be parallel to each other and
Set gradually along the length direction of detecting pad 3.Detection line T1 in three detections line T1, T2, T3 close to pad 2 is drop calcium
Plain former pairing antibody or antigen coating, the detection line T3 close to sample suction pad 4 are that the pairing antibody of N-terminal brain natriuretic peptide or antigen apply
Layer, middle detection line T2 be the gelatinase-associated lipocalin protein of neutrophil leucocyte pairing antibody or antigen coating, matter
Control line C is located at the end of the close sample suction pad 4 of detecting pad 3.Nature controlling line C includes but is not limited to sheep anti-mouse igg, goat-anti by coating
The formation such as chicken IgY or goat anti-rabbit igg.
Each part in this example is so assemblied together, and in hothouse, 20~25 DEG C of temperature, humidity is less than
40%, bottom plate 5 is taken, loading pad 1, pad 2, boosting pad 7, detecting pad 3 and sample suction pad 4, each several part are sequentially bonded on bottom plate 5
Successively with and be only in contact and partly overlap with adjacent regions, by coated detecting pad 3 be placed on bottom plate 5 middle part paste,
Pad is cut into suitable width, in detecting pad 3 close to detection line side overlap joint boosting pad 7, pad 2 is cut into conjunction
Suitable width, it is pasted onto at the 1/4 of boosting pad 7, is overlapped in the opposite side of pad 2 and paste loading pad 1, take pad 2 1/3 is viscous
Patch；The covering of hemofiltration film 6 is pasted onto on loading pad 1；Sample suction pad 4 is overlapped in the close nature controlling line side of detecting pad 3, takes sample suction pad 4
1/10 paste.The bottom plate posted is finally cut into the wide test strips of 3~5mm with cutter, loads and inspection is formed in plastic clip
Survey card.
The detection method of this example kit is as follows（Dry method loading）：
(1) detection reagent and sample are balanced to room temperature, takes out detection card, keep flat；
(2) sample to be tested is drawn, adds in the centrifuge tube of cleaning, is diluted with Sample dilution, is fully mixed；
(3) sample after drawing dilution with liquid-transfering gun is added in the sample aperture of loading pad 1, after 15 minutes, according to pre-
The standard curve for being first arranged on time-resolved fluoroimmunoassay quantitative readout instrument is calculated and shown to PCT, NGAL and BNP concentration
Show result.
The Cleaning Principle of this example detection card is as follows：
After sample is added to sample well, PCT, NGAL or NT-proBNP antigen and fluorescent microsphere mark that contain in sample
PCT, NGAL or NT-proBNP antibody binding of note, by capillarity, the antibody-antigen immune compound edge formed
Nitrocellulose filter is chromatographed to detection line, is then occurred with PCT, NGAL and NT-pro BNP antibody being solidificated in detection line high
The immune response of specificity and high-affinity, thus immune complex is enriched with or is trapped in the detection band of chromatographic material,
Uncombined immune complex then continues chromatography and captured to nature controlling line by sheep anti-mouse igg, goat-anti chicken IgY or goat anti-rabbit igg antibody.
After the completion of reaction, Eu in detection band3 +The content of chelate has certain corresponding relation with the concentration of target checking matter.Such as
Checking matter is free of in fruit testing sample solution, then does not just have Eu in detection band3 +Chelate.Due to lanthanide ion chelate
Fluorescence decay time length, be conventional fluorescent 103—106Times.When measuring its fluorescence with time-resolved fluorescence instrument, in pulsed light
After source excitation, it can postpone to measure again for a period of time, now the short-half-life fluorescence of other compositions has decayed, and only deposits
In Eu3+The specificity fluorescent of label, by measuring after immune response Eu on ELISA test strip band3 +The content of chelate,
Reference standard concentration curve can quantitatively obtains corresponding to the concentration of antigen in testing sample.
After reaction terminates, by analysis, according to being set in advance in time-resolved fluoroimmunoassay quantitative readout instrument
Standard curve to PCT, NGAL and NT-proBNP concentration carries out that result is calculated and be shown.
Embodiment 2
As shown in Fig. 2 this example provides a kind of multi objective time-resolved fluoroimmunoassay chromatography examination for postoperative patient monitoring
Agent box, its substantially with embodiment 1, unlike, its pad does not have time-resolved fluorescence microballoon labelled antibody coating, reagent
Box still further comprises time-resolved fluorescence microballoon labelled antibody solution.
The detection method of this example kit is as follows（Wet method loading）：
(1) detection reagent and sample are balanced to room temperature, takes out detection card, keep flat；
(2) sample to be tested is drawn, is sufficiently mixed with time-resolved fluorescence microballoon labelled antibody solution；
(3) draw mixed liquor with liquid-transfering gun to be added in the sample aperture of loading pad 1, after 15 minutes, according to having pre-set
PCT, NGAL and NT-proBNP concentration are calculated and shown in the standard curve of time-resolved fluoroimmunoassay quantitative readout instrument
Show result.
Embodiment 3
As shown in figure 3, this example provides a kind of multi objective time-resolved fluoroimmunoassay chromatography examination for postoperative patient monitoring
Agent box, its substantially with embodiment 1, unlike, its detecting pad divides pad 30,30 ', 30 " including three detections being arranged side by side, often
Individual detection divides on pad 30,30 ', 30 " forms a detection line T1 respectively, T2, T3 and nature controlling line C, C ', C ".
Preparation, detection and the Cleaning Principle of this example kit are the same as embodiment 1.
Embodiment 4
As shown in Fig. 4, Fig. 5, Fig. 6, this example provides a kind of multi objective time-resolved fluorescence for postoperative patient monitoring and exempted from
Epidemic disease chromatographs kit, its substantially with embodiment 1, unlike, detection card includes three independent detections point and blocked, hemofiltration film 6,
6 ', 6 ", loading pad 1,1 ', 1 ", pad 2,2 ', 2 ", boosting pad 7,7 ', 7 ", detection divide pad 30,30 ', 30 ", sample suction pad 4,
4 ', 4 " and bottom plate 5,5 ', 5 " have three respectively, each hemofiltration film 6,6 ', 6 ", loading pad 1,1 ', 1 ", pad 2,2 ', 2 ",
Boosting pad 7,7 ', 7 ", detection divide pad 30,30 ', 30 ", sample suction pad 4,4 ', 4 " and bottom plate 5,5 ', 5 " to be assembled into a detection point
Card, the detection that each detection point blocks divides on pad 30,30 ', 30 " forms a detection line T1, T2, T3 and nature controlling line C respectively,
C’,C”。
Preparation, detection and the Cleaning Principle of this example kit are the same as embodiment 1.
To sum up, the utility model is established using PCT, NGAL, NT-proBNP in serum, blood plasma or whole blood as purpose thing
Fluorescent microsphere time resolution immunochromatography detection method, combine time-resolved fluorescence microballoon high sensitivity, high specific and exempt from
Epidemic disease chromatographic technique can carry out single part of Samples detection, simple and rapid advantage, monitor while can be effectively inflammation after openheart surgery,
Renal failure and the risk of heart failure, simple to operate, 15 min can draw experimental result；It is supporting fluorescence detector economy, small-sized, more
Add suitable grass-roots unit；Both it is beneficial to larger scale clinical sample census screening and the monitoring of conditions of patients, again can single part operation.It is glimmering
Light microballoon time resolution immunochromatography is as a kind of sensitive, quick, economic, easy PCT, NGAL, NT-proBNP detection side
Method, there is extraordinary application value.
The utility model kit, suitable for whole blood test, is defended by increasing hemofiltration film after being easy to patient discharge in community
Life structure carries out cardiorenal function and infection detects, and mitigation goes to hospital to register, is lined up, chemically examining, taking the complicated processes such as report, mitigates public
The pressure of medical institutions altogether, is relatively beneficial to the daily monitoring of patient.
Further, the utility model adds boosting pad in structure, can effectively remove ambient interferences, so as to enter
One step improves diagnostic sensitivity and the degree of accuracy, and boosting pad additionally aids quickening detection sample flow in addition, improves detection efficiency, contracting
Short detection time.
The utility model is described in detail above, its object is to allow the personage for being familiar with this art can be much of that
Solve content of the present utility model and be carried out, the scope of protection of the utility model can not be limited with this, it is all according to this practicality
The equivalent change or modification that new Spirit Essence is made, it should all cover in the scope of protection of the utility model.
Claims (10)
1. a kind of multi objective time-resolved fluoroimmunoassay chromatography kit for postoperative patient monitoring, it includes detection card and can
The fluorescence immunoassay quantitative analysis instrument of selection, the detection card include loading pad（1）, pad（2）, there is detection line and nature controlling line
（C）Detecting pad（3）, sample suction pad（4）And bottom plate（5）, it is characterised in that：The index includes Procalcitonin, neutrophil leucocyte
Gelatinase-associated lipocalin protein and N-terminal brain natriuretic peptide, the pad（2）Including with reference to pad body（20）With coated on
Time-resolved fluorescence microballoon labelled antibody coating on the combination pad body（21）, wherein the time-resolved fluorescence microballoon mark
Remember the antibody that the antibody in antibody coating is the index；The detection line has three, this three detections line（T1、T2、T3）Phase
Mutually separation and the pairing antibody or antigen shape that can respectively be specifically bound with the time-resolved fluorescence microballoon labelled antibody
Into coating.
2. a kind of multi objective time-resolved fluoroimmunoassay chromatography kit for postoperative patient monitoring, it includes detection card and can
The fluorescence immunoassay quantitative analysis instrument of selection, the detection card include loading pad（1）, pad（2）, there is detection line and nature controlling line
（C）Detecting pad（3）, sample suction pad（4）And bottom plate（5）, it is characterised in that：The index includes Procalcitonin, neutrophil leucocyte
Gelatinase-associated lipocalin protein and N-terminal brain natriuretic peptide, the kit also include fluorescent labeled antibody solution, wherein institute
State the antibody that the antibody in time-resolved fluorescence microballoon labelled antibody coating is the index；The detection line has three, and this three
Bar detection line（T1、T2、T3）It is separated from each other and can be respectively tied with time-resolved fluorescence microballoon labelled antibody specificity
The coating that the pairing antibody or antigen of conjunction are formed.
3. the multi objective time-resolved fluoroimmunoassay chromatography reagent according to claim 1 or 2 for postoperative patient monitoring
Box, it is characterised in that：The detection card also includes hemofiltration film（6）, the hemofiltration film（6）It is covered in the loading pad（1）On, institute
State loading pad（1）, pad（2）, detecting pad（3）And sample suction pad（4）It is interlaced successively to be pasted onto bottom plate（5）On.
4. the multi objective time-resolved fluoroimmunoassay chromatography reagent according to claim 1 or 2 for postoperative patient monitoring
Box, it is characterised in that：The detection card also includes boosting pad（7）, and the detection card is by the loading pad（1）, pad
（2）, boosting pad（7）, detecting pad（3）And sample suction pad（4）It is interlaced successively to be pasted onto the bottom plate（5）Upper composition, it is described to help
Pushing pad（7）Material be glass fibre element film or non-woven fabrics.
5. the multi objective time-resolved fluoroimmunoassay chromatography reagent according to claim 1 or 2 for postoperative patient monitoring
Box, it is characterised in that：The detection card also includes hemofiltration film（6）With boosting pad（7）, the hemofiltration film（6）It is covered on described
Sample pad（1）On, the loading pad（1）, pad（2）, boosting pad（7）, detecting pad（3）And sample suction pad（4）It is interlaced successively
It is pasted onto bottom plate（5）On, the boosting pad（7）Material be glass fibre element film or non-woven fabrics.
6. the multi objective time-resolved fluoroimmunoassay chromatography reagent according to claim 1 or 2 for postoperative patient monitoring
Box, it is characterised in that：The detecting pad only has one, the three detections line（T1、T2、T3）, nature controlling line（C）Be parallel to each other and
Along the detecting pad（3）Length direction be successively set on the detecting pad.
7. the multi objective time-resolved fluoroimmunoassay chromatography kit according to claim 6 for postoperative patient monitoring,
It is characterized in that：The three detections line（T1、T2、T3）In close to pad（2）Detection line（T1）For matching somebody with somebody for Procalcitonin
To antibody or antigen coating, close to sample suction pad（4）Detection line（T3）For the pairing antibody or antigen coating of N-terminal brain natriuretic peptide,
Middle detection line（T2）It is described for the pairing antibody or antigen coating of the gelatinase-associated lipocalin protein of neutrophil leucocyte
Nature controlling line（C）Positioned at the detecting pad（3）The close sample suction pad（4）End.
8. the multi objective time-resolved fluoroimmunoassay chromatography reagent according to claim 1 or 2 for postoperative patient monitoring
Box, it is characterised in that：The detecting pad（3）Including three detection point pads (30,30 ', 30 "), the three detections line（T1、T2、
T3）It is respectively distributed on three detection point pads (30,30 ', 30 ").
9. the multi objective time-resolved fluoroimmunoassay chromatography kit according to claim 8 for postoperative patient monitoring,
It is characterized in that：The detection card includes three independent detections point card, the loading pad（1,1 ', 1 ' '）, pad（2,2’,
2’’）, sample suction pad（4,4 ', 4 ' '）And bottom plate（5,5 ', 5 ' '）There are three respectively, each loading pad（1,1 ', 1 ' '）, pad
（2,2’,2’’）, sample suction pad（4,4 ', 4 ' '）And bottom plate（5,5 ', 5 ' '）It is assembled into the detection point card described in one.
10. the multi objective time-resolved fluoroimmunoassay chromatography reagent according to claim 1 or 2 for postoperative patient monitoring
Box, it is characterised in that：The nature controlling line（C）Formed by being coated with sheep anti-mouse igg, goat-anti chicken IgY or goat anti-rabbit igg, the inspection
Survey pad（3）For nitrocellulose filter and be 5~12um of aperture porous spline structure film；The loading pad（1）, pad（2）'s
Material is glass fibre element film or non-woven fabrics, the sample suction pad（4）Material be absorbent filter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109387640A (en) * | 2018-12-17 | 2019-02-26 | 江苏苏博生物医学科技南京有限公司 | A kind of time-resolved fluoroimmunoassay chromatograph test strip and preparation method thereof that primary dcreening operation can be carried out to meningitis type |
| CN109613273A (en) * | 2019-01-23 | 2019-04-12 | 博奥赛斯（天津）生物科技有限公司 | A kind of five indexes of hepatitis b time-resolved fluoroimmunoassay measurement in chromatography kit |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109387640A (en) * | 2018-12-17 | 2019-02-26 | 江苏苏博生物医学科技南京有限公司 | A kind of time-resolved fluoroimmunoassay chromatograph test strip and preparation method thereof that primary dcreening operation can be carried out to meningitis type |
| CN109387640B (en) * | 2018-12-17 | 2024-04-05 | 江苏苏博生物医学科技南京有限公司 | Time-resolved fluorescence immunochromatography test strip capable of carrying out primary screening on meningitis types and preparation method thereof |
| CN109613273A (en) * | 2019-01-23 | 2019-04-12 | 博奥赛斯（天津）生物科技有限公司 | A kind of five indexes of hepatitis b time-resolved fluoroimmunoassay measurement in chromatography kit |
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