CN206638683U - Multi objective time-resolved fluoroimmunoassay for the monitoring of diabetes patient's cardiorenal function chromatographs kit - Google Patents

Abstract

It the utility model is related to a kind of time-resolved fluoroimmunoassay chromatography kit for the monitoring of diabetes patient's cardiorenal function, including detection card and selectable fluorescence immunoassay quantitative analysis instrument, detection card includes loading pad, pad, detecting pad with detection line and nature controlling line, sample suction pad and bottom plate, the index includes cardiac troponin, N-terminal brain natriuretic peptide and the gelatinase-associated lipocalin protein of neutrophil leucocyte, pad is included with reference to pad body and coated on the time-resolved fluorescence microballoon labelled antibody coating combined on pad body, antibody in the time-resolved fluorescence microballoon labelled antibody coating is the antibody of the index；Changed by the index for detecting CTnI, NT proBNP and NGAL simultaneously, timely, simplicity, Quantitative Monitoring intuitively, efficiently is carried out to heart failure, renal failure caused by diabetes, required sample size is few, high sensitivity, detection background signal is low, high specificity, the range of linearity is wide, easy to operate, reliable results.

Description

Multi objective time-resolved fluoroimmunoassay chromatography for the monitoring of diabetes patient's cardiorenal function Kit

Technical field

It the utility model is related to biotechnology diagnostic field, and in particular to a kind of to be used for what diabetes patient's cardiorenal function monitored Multi objective time-resolved fluoroimmunoassay chromatographs kit.

Background technology

Diabetes are the metabolic diseases characterized by chronic hyperglycemia.Counted according to the World Health Organization, it is diabetes complicated Disease is up to kind more than 100, and angiocardiopathy and nephrosis are the major complications of diabetes patient, and has a strong impact on diabetes patient's Life.According to statistics, caused by Diabetes Death person has more than half to be cardiovascular and cerebrovascular, caused by 10% is renal lesions.Clinical data shows Show, 10 years or so after onset diabetes, a kind of complication can at least be occurred into for the patient that have 30%~40%, and complication is once produced Raw, drug therapy is difficult to reverse, especially heart infarction and nephrosis.Therefore, the cardiovascular injury of early stage at-once monitor diabetes patient and Kidney injury risk, the generation of major disease such as heart infarction, end-stage renal disease can be prevented, save the life of patient in time.

CTnI is one of three subunits (cTnI, cTnC and cTnT) of cardiac troponin (cTn), is that musculature is received The regulatory protein of contracting, in the case of myocardial cell membrane is complete, cTnI can not appear cell membrane and enter blood circulation.Cardiac muscle cells When degeneration necrosis occurs because of ischemic, anoxic, cTnI is released into blood by damaged cell membrane.Because cTnI molecular weight is smaller and continues Escaped out of degeneration of cells, therefore after AMI occurs, occur in blood relatively early, and continue for quite a long time.With other sero-enzyme phases Than cTnI is the more special label of myocardial damage, while it is also very sensitive, can be performed the operation with Diagnosing Cardiac（Such as：Calcified Aortic stenosis and coronary bypass）There is 4h AMI in the small infarct and ischemic of surrounding.CTnI is in normal person's blood Content is very low in liquid, about 20.4pg/ml.cTnI >20.4pg/ml, just there is diagnostic significance to myocardial damage.Patient is occurring The minor myocardial damage of pectoralgia symptom a few days ago can be detected with cTnI.2.2-6.8 hours, it dense occurs in AMI Degree starts to raise, and about reaches peak value 195.9ng/ml at 11.2 hours, and the duration is long, can be down to after 5~10 days normal. CTnI has advantages below：Time of occurrence is early in blood, is more easy to be released into blood because molecular weight is small compared with CK-MB, after myocardial damage；Spirit Sensitivity is high；Specific high, Skeletal muscle injury does not influence the judgement of result, can speculate the type of surgery pair of non-cardiac surgery patient yet CTnI has no significant effect；Duration is grown, thus can myocardial damage early stage and later stage by detecting cTnI clarifying a diagnosis and Judge Myocardial injury degree.There is important clinical value in acute myocardial infarction diagnosis, treatment and prognosis.

NT-proBNP（N-terminal brain natriuretic peptide）, it is the product after proBNP (proBNP) is cut by endopeptidase, containing N-terminal 76 Individual amino acid.When heart failure occurs, BNP is discharged into blood, is then degraded to NT-proBNP and BNP, is detected in blood NT-proBNP and BNP content, the degree of heart failure can be monitored.Because NT-proBNP half-life period is longer (120min), Vitro stability is strong, and the concentration in heart failure patient is high compared with BNP, is more beneficial for the diagnosis of heart failure, therefore NT- Sensitive indicators of the proBNP as diagnosing patients with heart failure, and the evaluation of cardiac function infringement order of severity.U.S. FDA approval in 2002 The ElecsysproBNP listings of Roche companies, detect the NT-proBNP in blood.American Heart Association (AHA), U.S. clinical In " kit for diagnosing heart failure and treatment guidelines " and " application guide of cardiac marker " that biochemical institute (NACP) is formulated, NT- Diagnosis and prognostic indicator of the proBNP as heart failure.

The gelatinase-associated lipocalin protein of neutrophil leucocyte (NGAL) is a kind of small-molecular-weight secreted protein, by Kjeldsedn et al. had found in 1993, was the newcomer of Lipocalin families.Under physiological condition, NCAL synthesizes in bone on a small quantity The epithelial cell of multiple systems such as marrow neutrophil leucocyte and renal tubule, inflammatory reaction and malignant tumour can lure that it is being organized more into Largely generated in (such as uterus, prostate, salivary gland, lung, kidney, air flue and alimentary canal epithelium), can reaches in several hours To peak value, produce speed and be significantly faster than that other relevant biomarkers things.NGAL has just expressed in acute tubular damage early stage, Reflect that renal tubular function is disorderly, renal damage is prompted in the rise of its concentration, and the rise of traditional index serum creatinine then needs 24 More than hour.NGAL is also the preferred index thing of current new A KI diagnosis.

In the prior art, the Testing index of diabetes is based on blood glucose, although diabetic complication has a strong impact on patient's Quality of life, but diabetes patient and clinician often lay particular emphasis on the management of blood glucose, ignore cardiovascular and kidney injury prison Survey.On the one hand, prior art is single index detection mostly, and as pectoralgia detects enzymes, hematuria just goes detection kidney index； On the other hand, existing detection technique is not perfect enough, and platform technology is not general, such as CTnI, NGAL or NT-proBNP detection Reagent is clinically applied in different forms, and producing principle and operation sequence are different, and compatibility is poor；Third, early stage Monitoring index application is not popularized, and patient has been the late period of heart infarction or renal failure when often finding, be greatly affected to patient's Accurate Diagnosis, so as to cause many patients because of delay treatment and sb.'s illness took a turn for the worse even results in death.

The defects of to overcome above-mentioned technology, the utility model, which provides one kind, can realize heart injury of kidney synchronously, quickly, i.e. When the kit that monitors, realize that diabetes patient detects whenever and wherever possible in family, home for destitute, community sanitary institute, emergency treatment etc., avoid disliking The generation of property heart injury of kidney.

The content of the invention

Technical problem to be solved in the utility model is to provide a kind of time for the monitoring of diabetes patient's cardiorenal function Resolved fluorometric immune chromatography reagent kit.

To solve above technical problem, the utility model adopts the following technical scheme that：

A kind of multi objective time-resolved fluoroimmunoassay chromatography kit for the monitoring of diabetes patient's cardiorenal function, it includes Detection card and selectable fluorescence immunoassay quantitative analysis instrument, detection card include loading pad, pad, have detection line and nature controlling line Detecting pad, sample suction pad and bottom plate, Testing index includes cardiac troponin（CTnI）, N-terminal brain natriuretic peptide（NT-proBNP) and The gelatinase-associated lipocalin protein of neutrophil leucocyte（NGAL）, pad is including with reference to pad body and coated on reference to advance capital for Time-resolved fluorescence microballoon labelled antibody coating on body, the antibody in the time-resolved fluorescence microballoon labelled antibody coating is institute State the antibody of index；Detection line has three, and this three detections line is separated from each other and respectively can be with time-resolved fluorescence microballoon The coating that the pairing antibody or antigen of labelled antibody specific binding are formed.

Monoclonal antibody corresponding with CTnI, NGAL, NT-proBNP respectively, polyclonal antibody are coated with detecting pad, Or the nature controlling line that the antibody such as the detection line and sheep anti-mouse igg of antigen composition, goat-anti chicken IgY or goat anti-rabbit igg is formed accordingly.

The utility model also provides another time-resolved fluoroimmunoassay chromatography for being used for the monitoring of diabetes patient's cardiorenal function The scheme of kit, the kit include detection card and selectable fluorescence immunoassay quantitative analysis instrument, detection card include loading pad, Pad, detecting pad, sample suction pad and the bottom plate with detection line and nature controlling line, index include cardiac troponin, N-terminal brain sodium Peptide and the gelatinase-associated lipocalin protein of neutrophil leucocyte, kit also include fluorescent labeled antibody solution, wherein time Antibody in resolved fluorometric microballoon labelled antibody coating is the antibody of the index；Detection line has three, this three detections line phase Mutually separation and the pairing antibody or antigen that can respectively be specifically bound with time-resolved fluorescence microballoon labelled antibody are formed Coating.

According to a specific aspect of the present utility model, detection blocks by loading pad, pad, detecting pad and sample suction pad successively Interlaced be pasted onto on bottom plate is formed.

According to a preferred aspect of the present utility model, detection card also includes hemofiltration film, and hemofiltration film is covered on loading pad, Loading pad, pad, detecting pad and sample suction pad are interlaced successively to be pasted onto on bottom plate.

According to another preferred aspect of the present utility model, detection card also includes boosting pad, and detects card by loading pad, combination Interlaced be pasted onto on bottom plate is formed successively for pad, boosting pad, detecting pad and sample suction pad.

According to an also preferred aspect of the present utility model, detection card also includes hemofiltration film and boosting pad, and hemofiltration film is covered in On loading pad, loading pad, pad, boosting pad, detecting pad and sample suction pad is interlaced successively is pasted onto on bottom plate.

Preferably, the material of boosting pad is glass fibre element film or non-woven fabrics.

According to a preferred aspect of the present utility model, the detecting pad only has one, the three detections line, nature controlling line It is parallel to each other and is successively set on along the length direction of the detecting pad on the detecting pad.

Further, the detection line of close pad is the pairing antibody or antigen of cardiac troponin in three detections line Coating, the detection line close to sample suction pad apply for the pairing antibody or antigen of the gelatinase-associated lipocalin protein of neutrophil leucocyte Layer, middle detection line are the pairing antibody or antigen coating of N-terminal brain natriuretic peptide, and nature controlling line is located at the close sample suction pad of detecting pad End.

According to another preferred aspect of the present utility model, the detecting pad includes three detection point pads, three detections Line is respectively distributed on three detection point pads.Nature controlling line is respectively arranged with each detection point pad.CTnI、NT-proBNP、NGAL Corresponding monoclonal antibody, polyclonal antibody, or corresponding antigen are coated on three detecting pads respectively, on every detecting pad Be arranged in parallel nature controlling line, the antibody such as coating sheep anti-mouse igg, goat-anti chicken IgY or goat anti-rabbit igg, or only passes through glass to pad Treatment fluid processing, prepare the CTnI monoclonal antibodies comprising fluorescent microsphere mark, the NT-proBNP monoclonals of fluorescent microsphere mark The fluorescent liquid of the NGAL monoclonal antibodies of antibody and fluorescent microsphere mark.

According to another preferred aspect of the present utility model, detection card includes three independent detections point card, loading pad, combines Pad, sample suction pad and bottom plate have three respectively, and each loading pad, pad, detection point pad, sample suction pad and bottom plate are assembled into one Detection point card.

Preferably, nature controlling line includes but is not limited to the shapes such as sheep anti-mouse igg, goat-anti chicken IgY or goat anti-rabbit igg by coating Into.

Preferably, detecting pad is nitrocellulose filter and is 5~12um of aperture porous spline structure film；Loading pad, combine The material of pad is glass fibre element film or non-woven fabrics, and the material of sample suction pad is absorbent filter.

Fluorescent microsphere TIME RESOLVED TECHNIQUE and immuno-chromatographic assay technology are known.Time described in the utility model point Distinguish that fluorescent microsphere labelled antibody can use method well known in the art to prepare.

Due to the implementation of above technical scheme, the utility model has the following advantages that compared with prior art：This practicality is new The innovative point of type is by while detects CTnI, NT-proBNP and NGAL index change, timely, easy, directly perceived, quick Quantitative Monitoring is synchronized to the generation of heart failure and renal failure caused by the major diseases such as angiocarpy, diabetes, development, can realize Family detects and Site Detection, and required sample size is few, high sensitivity, and detection background signal is low, and high specificity, the range of linearity is wide, It is easy to operate, reliable results.

The utility model detection card allows it conveniently, simply, to be accurately applied to painstaking effort by increasing hemofiltration film The generation of heart failure and renal failure, development synchronize Quantitative Monitoring caused by the major diseases such as pipe, diabetes, suitable for family, support The simple and crude community sanitary institute of old institute, condition.Its application method is also only to be bled by patient or family members' acupuncture one to realize to painstaking effort The generation of heart failure and renal failure, development synchronize Quantitative Monitoring caused by the major diseases such as pipe, diabetes, and mitigation goes to hospital to hang Number, be lined up, chemically examine, taking the complicated processes such as report, mitigate the pressure of public medical mechanism, be relatively beneficial to the daily monitoring of patient.

Further, the utility model adds boosting pad in structure, can effectively remove ambient interferences, so as to enter One step improves diagnostic sensitivity height and the degree of accuracy, and boosting pad additionally aids quickening detection sample flow in addition, improves detection efficiency, Shorten detection time.

Brief description of the drawings

Fig. 1 is the main structure diagram blocked according to the detection of embodiment 1 of the present utility model；

Fig. 2 is the main structure diagram blocked according to the detection of embodiment 2 of the present utility model；

Fig. 3 is the dimensional structure diagram blocked according to the detection of embodiment 3 of the present utility model；

Fig. 4 is the main structure diagram according to the detection point card of embodiment 4 of the present utility model（Detection line containing T1）；

Fig. 5 is the main structure diagram according to the detection point card of embodiment 4 of the present utility model（Detection line containing T2）；

Fig. 6 is the main structure diagram according to the detection point card of embodiment 4 of the present utility model（Detection line containing T3）；

Wherein：1,1 ', 1 ", loading pad；2,2 ', 2 ", gold conjugation pad；20,20 ', 20 ", with reference to pad body；21, 21 ', 21 ", colloidal gold labeled monoclonal antibody coating；3rd, detecting pad；30,30 ', 30 ", detection point pad；4,4 ', 4 ", sample suction pad；5,5’, 5 ", bottom plate；6,6 ', 6 ", hemofiltration film；7,7 ', 7 ", boosting pad；T1, T2, T3, detection line；C, C ', C ", nature controlling line.

Embodiment

Embodiment 1

As shown in figure 1, this example provides a kind of time-resolved fluoroimmunoassay chromatography for the monitoring of diabetes patient's cardiorenal function Kit, it includes detection card, fluorescence immunoassay quantitative analysis instrument, detection card includes loading pad 1, pad 2, with detection line and Nature controlling line C detecting pad 3, sample suction pad 4 and bottom plate 5, hemofiltration film 6 and boosting pad 7, hemofiltration film 6 are covered on loading pad 1, loading Pad 1, pad 2, detecting pad 3 and sample suction pad 4 are interlaced successively to be pasted onto on bottom plate 5.Wherein, the material of boosting pad 7 is glass Glass cellulose membrane or non-woven fabrics.Detecting pad 3 is nitrocellulose filter and is 5~12um of aperture porous spline structure film；Loading pad 1st, the material of pad 2 is glass fibre element film or non-woven fabrics, and the material of sample suction pad 4 is absorbent filter.

Further, detection card can detect cardiac troponin, N-terminal brain natriuretic peptide and neutrophil leucocyte gelatinase simultaneously Three kinds of indexs of correlation lipocalin protein, pad 2 include with reference to pad body 20 and coated on combine pad body 20 on when Between resolved fluorometric microballoon labelled antibody coating 21, the antibody in the time-resolved fluorescence microballoon labelled antibody coating 21 is the finger Target antibody.The detecting pad 3 is one, has three detections line T1, T2, T3 thereon, this three detections line T1, T2, T3 mutually divide From and respectively three kinds of marks the coating that is formed of pairing antibody or antigen, the pairing antibody or antigen difference of three kinds of marks It can be specifically bound with time-resolved fluorescence microballoon labelled antibody coating.Three detections line T1, T2, T3, nature controlling line C are mutually flat Go and set gradually along the length direction of detecting pad 3.The detection line T1 of close pad 2 is in three detections line T1, T2, T3 The pairing antibody or antigen coating of cardiac troponin, the detection line T3 close to sample suction pad 4 are related for neutrophil leucocyte gelatinase Property lipocalin protein pairing antibody or antigen coating, middle detection line T2 is the pairing antibody or anti-of N-terminal brain natriuretic peptide Former coating, nature controlling line C are located at the end of the close sample suction pad 4 of detecting pad 3.Nature controlling line C includes but is not limited to goat-anti by coating The formation such as mouse IgG, goat-anti chicken IgY or goat anti-rabbit igg.

Each part in this example is so assemblied together, and in hothouse, 20~25 DEG C of temperature, humidity is less than 40%, bottom plate 5 is taken, the middle part that coated detecting pad 3 is placed on to bottom plate 5 is pasted, and pad is cut into suitable width, In detecting pad 3 close to detection line side overlap joint boosting pad 7, pad 2 is cut into suitable width, is pasted onto boosting pad 7 At 1/4, overlapped in the opposite side of pad 2 and paste loading pad 1, take pad 2 1/3 is pasted；The loading pad that hemofiltration film 6 is covered in On 1；Sample suction pad 4 is overlapped in the close nature controlling line side of detecting pad 3, take sample suction pad 4 1/10 is pasted.It will finally be pasted with cutter Good bottom plate is cut into the wide test strips of 3~5mm, loads and detection card is formed in plastic clip.

The detection method of this example kit is as follows（Dry method loading）：

(1) detection reagent and sample are balanced to room temperature, takes out detection card, keep flat；

(2) sample to be tested is drawn, adds in the centrifuge tube of cleaning, is diluted with Sample dilution, is fully mixed；

(3) sample after drawing dilution with liquid-transfering gun is added in the sample aperture of loading pad 1, after 15 minutes, according to pre- The standard curve for being first arranged on time-resolved fluoroimmunoassay quantitative readout instrument is carried out to CTNI, NGAL and NT-proBNP concentration Result is calculated and be shown.

The Cleaning Principle of this example detection card is as follows：

After sample is added to sample well, CTnI, NT-proBNP or NGAL antigen and fluorescent microsphere mark that contain in sample CTnI, NT-proBNP or NGAL antibody binding of note, by capillarity, the antibody-antigen immune compound edge formed Nitrocellulose filter is chromatographed to detection line, is then occurred with CTnI, NT-proBNP and NGAL antibody being solidificated in detection line high The immune response of specificity and high-affinity, thus immune complex is enriched with or is trapped in the detection band of chromatographic material, Uncombined immune complex then continues chromatography and caught to nature controlling line by antibody such as sheep anti-mouse igg goat-anti chicken IgY or goat anti-rabbit igg Obtain.After the completion of reaction, Eu in detection band ^{3 +}The content of chelate has certain corresponding relation with the concentration of target checking matter.Such as Checking matter is free of in fruit testing sample solution, then does not just have Eu in detection band^{3 +}Chelate.Due to lanthanide ion chelate Fluorescence decay time length, be conventional fluorescent 10³—10⁶Times.When measuring its fluorescence with time-resolved fluorescence instrument, in pulsed light After source excitation, it can postpone to measure again for a period of time, now the short-half-life fluorescence of other compositions has decayed, and only deposits In Eu³⁺The specificity fluorescent of label, by measuring after immune response Eu on ELISA test strip band ^{3 +}The content of chelate, Reference standard concentration curve can quantitatively obtains corresponding to the concentration of antigen in testing sample.

After reaction terminates, analyzed, according to being set in advance in time-resolved fluoroimmunoassay quantitative readout instrument Standard curve to CTnI, NT-proBNP and NGAL concentration carries out that result is calculated and be shown.

Embodiment 2

As shown in Fig. 2 this example provides a kind of time-resolved fluoroimmunoassay chromatography for the monitoring of diabetes patient's cardiorenal function Kit, its substantially with embodiment 1, unlike, its pad have time-resolved fluorescence microballoon labelled antibody coating, tries Agent box still further comprises time-resolved fluorescence microballoon labelled antibody solution.

The detection method of this example kit is as follows（Wet method loading）：

(1) detection reagent and sample are balanced to room temperature, takes out detection card, keep flat；

(2) sample to be tested is drawn, is sufficiently mixed with time-resolved fluorescence microballoon labelled antibody solution；

(3) draw mixed liquor with liquid-transfering gun to be added in the sample aperture of loading pad 1, after 15 minutes, according to having pre-set CTnI, NT-proBNP and NGAL concentration are calculated simultaneously in the standard curve of time-resolved fluoroimmunoassay quantitative readout instrument Show result.

Embodiment 3

As shown in figure 3, this example provides a kind of time-resolved fluoroimmunoassay chromatography for the monitoring of diabetes patient's cardiorenal function Kit, its substantially with embodiment 1, unlike, its detecting pad 3 divides pad 30,30 ' including three detections being arranged side by side, 30 ", each detection divides on pad 30,30 ', 30 " forms a detection line and nature controlling line respectively.

Preparation, detection and the Cleaning Principle of this example kit are the same as embodiment 1.

Embodiment 4

As shown in Fig. 4, Fig. 5, Fig. 6, this example provides a kind of time-resolved fluorescence for the monitoring of diabetes patient's cardiorenal function Immune chromatography reagent kit, its substantially with embodiment 1, unlike, detection card includes three independent detections point and blocked, hemofiltration film 6, 6 ', 6 ", loading pad 1,1 ', 1 ", pad 2,2 ', 2 ", boosting pad 7,7 ', 7 ", detection divide pad 30,30 ', 30 ", sample suction pad 4, 4 ', 4 " and bottom plate 5,5 ', 5 " have three respectively, each hemofiltration film 6,6 ', 6 ", loading pad 1,1 ', 1 ", pad 2,2 ', 2 ", Boosting pad 7,7 ', 7 ", detection divide pad 30,30 ', 30 ", sample suction pad 4,4 ', 4 " and bottom plate 5,5 ', 5 " to be assembled into a detection point Card, the detection that each detection point blocks divides on pad 30,30 ', 30 " forms a detection line and nature controlling line respectively.

Preparation, detection and the Cleaning Principle of this example kit are the same as embodiment 1.

To sum up, innovative point of the present utility model is by while detects the index change of CTnI, NT-proBNP and NGAL sum Change, in time, it is easy, directly perceived, efficiently to the generation of heart failure and renal failure caused by the major diseases such as angiocarpy, diabetes, develop Quantitative Monitoring is synchronized, family's detection and Site Detection can be realized, required sample size is few, high sensitivity, detects background signal Low, high specificity, the range of linearity is wide, easy to operate, reliable results, and the accuracy rate of clinical monitoring is up to more than 95%.

The utility model detection card allows it conveniently, simply, to be accurately applied to diabetes by increasing hemofiltration film The generation of caused heart failure and renal failure, development synchronize Quantitative Monitoring, suitable for the simple and crude community of family, home for destitute, condition Health-center.Its application method be also only bled by patient or family members' acupuncture one realize to the generation of heart failure and renal failure, develop into The synchronous Quantitative Monitoring of row, mitigation go to hospital to register, are lined up, chemically examining, taking the complicated processes such as report, mitigate the pressure of public medical mechanism Power, it is relatively beneficial to the daily treatment of patient.

Further, the utility model adds boosting pad in structure, can effectively remove ambient interferences, so as to enter One step improves diagnostic sensitivity and the degree of accuracy, and boosting pad additionally aids quickening detection sample flow in addition, improves detection efficiency, contracting Short detection time.

The utility model is described in detail above, its object is to allow the personage for being familiar with this art can be much of that Solve content of the present utility model and be carried out, the scope of protection of the utility model can not be limited with this, it is all according to this practicality The equivalent change or modification that new Spirit Essence is made, it should all cover in the scope of protection of the utility model.

Claims (10)

1. a kind of multi objective time-resolved fluoroimmunoassay chromatography kit for the monitoring of diabetes patient's cardiorenal function, it includes inspection Card and selectable fluorescence immunoassay quantitative analysis instrument are surveyed, the detection card includes loading pad（1）, pad（2）, there is detection line And nature controlling line（C）Detecting pad（3）, sample suction pad（4）And bottom plate（5）, it is characterised in that：The index includes heart flesh calcium egg In vain, N-terminal brain natriuretic peptide and the gelatinase-associated lipocalin protein of neutrophil leucocyte, the pad（2）Including with reference to advance capital for Body（20）With coated on the time-resolved fluorescence microballoon labelled antibody coating on the combination pad body（21）, wherein the time Antibody in resolved fluorometric microballoon labelled antibody coating is the antibody of the index；The detection line has three, this three detections Line（T1、T2、T3）It is separated from each other and can respectively matches somebody with somebody with what the time-resolved fluorescence microballoon labelled antibody was specifically bound The coating formed to antibody or antigen.

2. a kind of multi objective time-resolved fluoroimmunoassay chromatography kit for the monitoring of diabetes patient's cardiorenal function, it includes inspection Card and selectable fluorescence immunoassay quantitative analysis instrument are surveyed, the detection card includes loading pad（1）, pad（2）, there is detection line And nature controlling line（C）Detecting pad（3）, sample suction pad（4）And bottom plate（5）, it is characterised in that：The index includes heart flesh calcium egg In vain, N-terminal brain natriuretic peptide and the gelatinase-associated lipocalin protein of neutrophil leucocyte, the kit also resist including fluorescence labeling Liquid solution, wherein the antibody in the time-resolved fluorescence microballoon labelled antibody coating is the antibody of the index；The detection Line has three, this three detections line（T1、T2、T3）It is separated from each other and can be respectively marked with the time-resolved fluorescence microballoon The coating that the pairing antibody or antigen that antibody specificity combines are formed.

3. the multi objective time-resolved fluoroimmunoassay according to claim 1 or 2 for the monitoring of diabetes patient's cardiorenal function Chromatograph kit, it is characterised in that：The detection card also includes hemofiltration film（6）, the hemofiltration film（6）It is covered in the loading pad （1）On, the loading pad（1）, pad（2）, detecting pad（3）And sample suction pad（4）It is interlaced successively to be pasted onto bottom plate（5） On.

4. the multi objective time-resolved fluoroimmunoassay according to claim 1 or 2 for the monitoring of diabetes patient's cardiorenal function Chromatograph kit, it is characterised in that：The detection card also includes boosting pad（7）, and the detection card is by the loading pad（1）、 Pad（2）, boosting pad（7）, detecting pad（3）And sample suction pad（4）It is interlaced successively to be pasted onto the bottom plate（5）Upper composition, The boosting pad（7）Material be glass fibre element film or non-woven fabrics.

5. the multi objective time-resolved fluoroimmunoassay according to claim 1 or 2 for the monitoring of diabetes patient's cardiorenal function Chromatograph kit, it is characterised in that：The detection card also includes hemofiltration film（6）With boosting pad（7）, the hemofiltration film（6）Cover In the loading pad（1）On, the loading pad（1）, pad（2）, boosting pad（7）, detecting pad（3）And sample suction pad（4）Successively It is interlaced to be pasted onto bottom plate（5）On, the boosting pad（7）Material be glass fibre element film or non-woven fabrics.

6. the multi objective time-resolved fluoroimmunoassay according to claim 1 or 2 for the monitoring of diabetes patient's cardiorenal function Chromatograph kit, it is characterised in that：The detecting pad only has one, the three detections line（T1、T2、T3）, nature controlling line（C）Phase It is mutually parallel and along the detecting pad（3）Length direction be successively set on the detecting pad.

7. the multi objective time-resolved fluoroimmunoassay chromatography according to claim 6 for the monitoring of diabetes patient's cardiorenal function Kit, it is characterised in that：The three detections line（T1、T2、T3）In close to pad（2）Detection line（T1）For heart flesh The pairing antibody or antigen coating of calcium albumen, close to sample suction pad（4）Detection line（T3）It is gelatinase-associated for neutrophil leucocyte The pairing antibody or antigen coating of lipocalin protein, middle detection line（T2）Pairing antibody or anti-for N-terminal brain natriuretic peptide Former coating, the nature controlling line（C）Positioned at the detecting pad（3）The close sample suction pad（4）End.

8. the multi objective time-resolved fluoroimmunoassay according to claim 1 or 2 for the monitoring of diabetes patient's cardiorenal function Chromatograph kit, it is characterised in that：The detecting pad（3）Including three detection point pads（30,30’,30”）, three detections Line（T1,T2,T3）It is respectively distributed to three detections point pad（30,30 ', 30 "）On.

9. the multi objective time-resolved fluoroimmunoassay chromatography according to claim 8 for the monitoring of diabetes patient's cardiorenal function Kit, it is characterised in that：The detection card includes three independent detections point card, the loading pad（1,1’,1”）, pad （2,2’,2”）, sample suction pad（4,4’,4”）And bottom plate（5,5’,5”）There are three respectively, each loading pad（1,1’,1”）, combine Pad（2,2’,2”）, detection point pad（30,30’,30”）, sample suction pad（4,4’,4”）And bottom plate（5,5’,5”）It is assembled into described in one Detection point card.

10. the multi objective time-resolved fluoroimmunoassay according to claim 1 or 2 for the monitoring of diabetes patient's cardiorenal function Chromatograph kit, it is characterised in that：The nature controlling line（C）By being coated with sheep anti-mouse igg, goat-anti chicken IgY or goat anti-rabbit igg shape Into the detecting pad（3）For nitrocellulose filter and be 5~12um of aperture porous spline structure film；The loading pad（1）, knot Close pad（2）Material be glass fibre element film or non-woven fabrics, the sample suction pad（4）Material be absorbent filter.