CN208060389U - A kind of multi objective time-resolved fluoroimmunoassay for myocardial infarction Quantitative detection chromatographs kit - Google Patents
A kind of multi objective time-resolved fluoroimmunoassay for myocardial infarction Quantitative detection chromatographs kit Download PDFInfo
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Abstract
The utility model is related to a kind of multi objective time-resolved fluoroimmunoassay for myocardial infarction Quantitative detection to chromatograph kit, including detection card and selectable fluorescence immunoassay quantitative analysis instrument, the Testing index includes cardiac troponin, creatine kinase isozyme and myoglobins；The utility model detects the index variation of cTnI, CK-MB and Myo using time-resolved fluoroimmunoassay chromatography simultaneously, Quantitative Monitoring more accurately is carried out to the generation of myocardial infarction, it can realize family's detection and Site Detection, required sample size is few, high sensitivity, high specificity, the range of linearity are wide, it is easy to operate, as a result reliably.
Description
Technical field
The utility model is related to biotechnology diagnostic fields, and in particular to a kind of for myocardial infarction Quantitative detection
Multi objective time-resolved fluoroimmunoassay chromatographs kit.
Background technology
Myocardial infarction is called myocardial infarction, and acute myocardial infarction (AMI) seriously threatens human health, is caused in global range
One of principal disease that is dead and disabling, quick diagnosis early stage acute myocardial infarction and in time treatment are to reduce the pass of patient death rate
Key.For no typical chest pain and the unconspicuous myocardial infarction patient of ECG change, electrocardiogram, echocardiogram are only relied on, it is difficult
With Accurate Diagnosis.Therefore, detection serum cardiac marker is the necessary foundation for diagnosing AMI.
Applications of Cardiac Markers refers to a series of biochemicals that can be determined in blood, they have cardiac muscle special
Property.It is largely discharged into blood circulation when myocardial tissue damage, by detecting its blood concentration variation diagnostic myocardial damage situation,
Therefore it can be used as the screening, diagnosis, the monitoring mark for evaluating prognosis and therapeutic effect of myocardial damage.It is sent out successively in clinical practice
The marker of existing a variety of reflecting myocardium tissue damages, includes the marker of reflecting myocardium ischemic injuries, as ischemia modified albumin IMA,
Myeloperoxidase etc.；The determination marker of myocardial tissue damage necrosis, such as cardiac troponin.Applications of Cardiac Markers is clinic
It provides a convenient, the ability of antidiastole, evaluation prognosis is made to greatly improve.
Applications of Cardiac Markers is mainly to assist AMI early diagnosis by the form of kit.Such existing kit exist with
Lower deficiency：Because the marker of selection is single or the scientific poor and kit technology restriction of marker combination, lead to specificity
Difference, detection sensitivity is low, the range of linearity is narrow etc., influences the efficiency and accuracy of diagnosis；It sues and labours to be difficult to realize make a definite diagnosis as early as possible
Purpose.
Therefore, it is badly in need of that a kind of detection sensitivity higher, the detection range of linearity be wider, the more accurate myocardial infarction of testing result
Marker detects product immediately.
Utility model content
Technical problem to be solved by the utility model is to provide a kind of sensitivity higher, the detection range of linearity is wider, examines
Survey the more accurate myocardial infarction rapid quantitative detection reagent box of result.Specifically it is to provide a kind of multi objective time-resolved fluoroimmunoassay
Kit is chromatographed, the index by detecting Blood Center flesh troponin, creatine kinase isozyme and myoglobins simultaneously becomes
Change, accurately detects early stage myocardial damage.Have the characteristics that high specificity, accuracy are high, with one bleed realize family detection and
Site Detection is conducive to carry out symptomatic treatment early, avoids the further generation of myocardial infarction, to protecting the lives and properties of patient
Safety is of great significance, while also having high social benefit.
In order to solve the above technical problems, the utility model provides a kind of multi objective for myocardial infarction Quantitative detection
Time-resolved fluoroimmunoassay chromatographs kit, and specific technical solution is as follows：
The kit includes detection card and selectable fluorescence immunoassay quantitative analysis instrument.The detection card includes loading
Pad, bonding pad, the detecting pad with detection line and nature controlling line, sample suction pad and bottom plate.Testing index includes cardiac troponin, flesh
Acid kinase isodynamic enzyme and myoglobins.Bonding pad includes in conjunction with pad body and coated on the time resolution on the combination pad body
Fluorescent microsphere labelled antibody coating, the antibody in the time-resolved fluorescence microballoon labelled antibody coating are the antibody of the index；
The detection line has three, which is separated from each other and is that can mark to resist with the time-resolved fluorescence microballoon respectively
The coating that the pairing antibody or antigen that the antibody specificity of body coating combines are formed.
The detection card further includes hydrophobic enclosure, there is well and inspection window on shell.
Above-mentioned cardiac troponin is preferably CTnI.CTnI is three subunits of cardiac troponin (cTn)
One of (cTnI, cTnC and cTnT) is the regulatory protein of contraction of muscle tissue, cTnI complete in myocardial cell membrane
Cell membrane cannot be appeared and enter blood circulation.When because of ischemic, anoxic degeneration necrosis occurs for cardiac muscle cells, cTnI passes through damaged cell
Film is released into blood.Since cTnI molecular weight is smaller and continues to escape out of degeneration of cells, therefore after AMI occurs, occur in blood compared with
It is early, and continue for quite a long time.Compared with other sero-enzymes, cTnI is the more special marker of myocardial damage, while it
It is very sensitive, the small infarct for the surrounding that can be performed the operation with Diagnosing Cardiac.CTnI contents in normal human blood are very low, about 20.4pg/
ml.cTnI>20.4pg/ml just has diagnostic significance to myocardial damage.Is there is the small cardiac muscle damage of pectoralgia symptom a few days ago in patient
Wound can be detected with cTnI.CTnI has the following advantages：Time of occurrence is early in blood；High sensitivity；It is specific high, bone
Injury of muscle does not influence the judgement of result, can speculate that the type of surgery of non-cardiac surgery patient has no significant effect cTnI yet；Continue
Time is long, therefore can be clarified a diagnosis in myocardial damage early stage and later stage by detecting cTnI and judge Myocardial injury degree.?
Acute myocardial infarction diagnosis, treatment and prognosis have important clinical value.
Myoglobins (Myo), is present in the striated muscle of heart bone.When myocardial damage, 1~3h of myoglobins
There is abnormal increase.Although Myo Cardiac-specifics are not high, promptly released from the cardiac muscle of necrosis after myocardial infarction,
Sensibility with height, Myo feminine genders are remarkably contributing to exclude the diagnosis of AMI.
Creatine kinase isozyme described in the utility model refers to Creatine Kinase MB (CK-MB), is creatine kinase
(CK) one of four kinds of isoenzymes forms (muscularity-MM, brain type-BB, hydridization type-MB and Mitochondrial form-MiMi).It is primarily present in
In cardiac muscle cell, when myocardial damage, CK-MB is released into blood circulation at once, and 3~4h starts to increase after myocardial damage,
It is down to normal range (NR) within 48~72 hours.
Three cardiac troponin, creatine kinase isozyme and myoglobins Testing index joint examining for myocardial infarction
It is disconnected, it is greatly improved the specificity and accuracy of detection.
The kit of the utility model, detection sensitivity higher, it is similar that detection line can reach existing above-mentioned marker
The 10-30% of product just can truly understand physical condition.
The fluorescent microsphere is selected from the polystyrene microsphere of modified, and the chelate of inside filling lanthanide series is selected from
Include one kind in europium (Eu), terbium (Tb), samarium (Sm), neodymium (Nd) or dysprosium (Dy).
Monoclonal antibody corresponding with cTnI, CK-MB and Myo, polyclonal antibody or phase respectively are coated on detecting pad
The nature controlling line that the detection line and mouse IgG antibody that the antigen answered is constituted are constituted.
Preferably, nature controlling line includes but not limited to the antibody such as sheep anti-mouse igg, goat-anti chicken IgY or goat anti-rabbit igg by coating
It is formed.
The utility model also provides a kind of multi objective time-resolved fluoroimmunoassay for myocardial infarction Quantitative detection
The scheme of kit is chromatographed, which includes detection card and selectable fluorescence immunoassay quantitative analysis instrument, the detection Ka Bao
Include loading pad, bonding pad, the detecting pad with detection line and nature controlling line, sample suction pad and bottom plate；Testing index includes myocardium myo calcium
Albumen, creatine kinase isozyme and myoglobins；The kit further includes time-resolved fluorescence microballoon labelled antibody solution, this when
Between antibody in resolved fluorometric microballoon labelled antibody solution be the index antibody；The detection line has three, this three inspections
Survey line be separated from each other and be respectively the pairing antibody that can be specifically bound with the time-resolved fluorescence microballoon labelled antibody or
The coating that antigen is formed.The bonding pad of the program is merely through the processing of glass treatment fluid, uncoated time-resolved fluorescence microballoon label
Antibody coating, it is another to prepare the cTnI monoclonal antibodies comprising fluorescent microsphere label, the CK-MB monoclonal antibodies of fluorescent microsphere label
With the fluorescent liquid of the Myo monoclonal antibodies of fluorescent microsphere label.
A specific aspect according to the present utility model, detection block by loading pad, bonding pad, detecting pad and sample suction pad successively
Interlaced be pasted onto on bottom plate is constituted.
A preferred aspect according to the present utility model, detection card further includes hemofiltration film, which is covered in loading pad
On, loading pad, bonding pad, detecting pad and sample suction pad are interlaced successively to be pasted onto on bottom plate.
Another preferred aspect according to the present utility model, detection card further includes boosting pad, and detects card by loading pad, combination
Interlaced be pasted onto on bottom plate is constituted successively for pad, boosting pad, detecting pad and sample suction pad.
An also preferred aspect according to the present utility model, detection card further includes hemofiltration film and boosting pad, and hemofiltration film is covered in
On loading pad, loading pad, bonding pad, boosting pad, detecting pad and sample suction pad is interlaced successively is pasted onto on bottom plate.
A preferred aspect according to the present utility model, the detecting pad only have one, the three detections line, a matter
Control line is mutually parallel and is successively set on the detecting pad along the length direction of the detecting pad.
Further, the nature controlling line is located at the end of the close sample suction pad of the detecting pad；Three detections
Close to the pairing antibody or antigen coating that the detection line of bonding pad is cardiac troponin in line, the detection line close to nature controlling line is
The pairing antibody or antigen coating of myoglobins, intermediate detection line are that the pairing antibody of creatine kinase isozyme or antigen apply
Layer.
Another preferred aspect according to the present utility model, the detecting pad include three detection point pads, three detections
Line is respectively distributed on three detection point pads.It is respectively arranged with nature controlling line on each detection point pad.CTnI, CK-MB and Myo are opposite
The monoclonal antibody answered, polyclonal antibody, or corresponding antigen are coated on respectively on three detection point pads, on every detection point pad
One of nature controlling line, the antibody such as coating sheep anti-mouse igg, goat-anti chicken IgY or goat anti-rabbit igg is arranged in parallel.
Another preferred aspect according to the present utility model, detection card include three independent detection point cards, and loading pad combines
Pad, sample suction pad and bottom plate respectively have three, and each loading pad, bonding pad, detection point pad, sample suction pad and bottom plate are assembled into an inspection
Survey a point card.Or, hemofiltration film, loading pad, bonding pad, boosting pad, sample suction pad and bottom plate respectively have three, each hemofiltration film, loading
Pad, bonding pad, boosting pad, boosting pad, detection point pad, sample suction pad and bottom plate are assembled into a detection point card.
Preferably, detecting pad is nitrocellulose filter, the porous spline structure film of further preferably 5~12um of aperture；On
Sample pad, bonding pad material be glass fibre element film or non-woven fabrics, the material of sample suction pad is absorbent filter, and the material of bottom plate is preferred
For plastics.
Fluorescent microsphere TIME RESOLVED TECHNIQUE and immuno-chromatographic assay technology are known, the time described in the utility model point
Distinguish that fluorescent microsphere labelled antibody can be used method well known in the art and prepare.
Due to the implementation of above technical scheme, the utility model has the following advantages that compared with prior art：
The innovative point of the utility model is, detected simultaneously using time-resolved fluoroimmunoassay chromatography cTnI, CK-MB and
The index of Myo changes, and more accurately carries out Quantitative Monitoring to the generation of myocardial infarction, can realize family's detection and scene inspection
It surveys, required sample size is few, and high sensitivity, high specificity, the range of linearity is wide, easy to operate, as a result reliably.
The utility model detection card passes through the kit that can be only applied to hospital laboratory originally by technological innovation
Increase hemofiltration film so that it can which the convenient, fast and accurate generation being applied to myocardial infarction, development synchronize quantitative prison
It surveys, is suitable for family, home for destitute, community sanitary institute, application method is bled by patient or family members' needle thorn one to realize
Self detection of myocardial infarction, improves the possibility of real-time self-monitoring, removes body from and slightly do not go to hospital to register, arrange in due course
Team chemically examines, takes the complicated processes such as report, mitigates the pressure of public medical mechanism, is relatively beneficial to the daily treatment of patient.
Further, the utility model increases boosting pad in structure, can effectively remove background interference, thus into
One step improves diagnostic sensitivity height and accuracy, and boosting pad additionally aids quickening detection sample flow in addition, improves detection efficiency,
Shorten detection time.
Description of the drawings
Fig. 1 is the main structure diagram of the detection card of the embodiments of the present invention 1；
Fig. 2 is the main structure diagram of the detection card of the embodiments of the present invention 2；
Fig. 3 is the dimensional structure diagram of the detection card of the embodiments of the present invention 3；
Fig. 4 is the main structure diagram (detection line containing T1) of the detection point card of the embodiments of the present invention 4；
Fig. 5 is the main structure diagram (detection line containing T2) of the detection point card of the embodiments of the present invention 4；
Fig. 6 is the main structure diagram (detection line containing T3) of the detection point card of the embodiments of the present invention 4.
Reference numeral：
1,1 ', 1 "-loading pad 2,2 ', 2 "-bonding pad, 3-detecting pad
30,30 ', 30 "-detections divide 4,4 ', 4 "-sample suction pad of pad 5,5 ', 5 "-bottom plate
6,6 ', 6 "-hemofiltration film, 7,7 ', 7 "-boosting pads T1, T2, T3-detection line
C, C ', C "-nature controlling lines 20,20 ', 20 "-combine pad body
21,21 ', 21 "-time-resolved fluorescence microballoon labelled antibody coatings
Specific implementation mode
Embodiment 1
As shown in Figure 1, the present embodiment provides a kind of multi objective time resolution for myocardial infarction Quantitative detection is glimmering
Light immune chromatography reagent kit comprising detection card, fluorescence immunoassay quantitative analysis instrument, detection card include loading pad 1, bonding pad 2, tool
There are detecting pad 3, sample suction pad 4, bottom plate 5, hemofiltration film 6 and the boosting pad 7 of detection line T and nature controlling line C, hemofiltration film 6 to be covered in loading
On pad 1, loading pad 1, bonding pad 2, boosting pad 7, detecting pad 3 and sample suction pad 4 are interlaced successively to be pasted onto on bottom plate 5.Wherein,
Detecting pad 3 be nitrocellulose filter and be 5~12um of aperture porous spline structure film；Loading pad 1, bonding pad 2 and boosting pad 7
Material is glass fibre element film or non-woven fabrics, and the material of sample suction pad 4 is absorbent filter.
Further, detection card can detect three kinds of cardiac troponin, creatine kinase isozyme and myoglobins simultaneously
Index, bonding pad 2 include in conjunction with pad body 20 and coated on the time-resolved fluorescence microballoon labelled antibody combined on pad body 20
Coating 21, the antibody in the time-resolved fluorescence microballoon labelled antibody coating 21 are cardiac troponin, creatine kinase isozyme
With the antibody of myoglobins.The detecting pad 3 is one, has three detections line T1, T2 and T3 thereon, this three detections line is mutually divided
From and respectively three kinds of markers the coating that is formed of pairing antibody or antigen, the pairing antibody or antigen difference of three kinds of markers
It can be specifically bound with time-resolved fluorescence microballoon labelled antibody coating.Three detections line T1, T2, T3 and nature controlling line C are mutual
It is parallel and set gradually along the length direction of detecting pad 3.Nature controlling line C is located at the end of the close sample suction pad 4 of detecting pad 3；Three
Close to the pairing antibody or antigen coating that the detection line T1 of bonding pad 2 is cardiac troponin in detection line T1, T2, T3, lean on
The detection line T3 of nearly nature controlling line C is the pairing antibody or antigen coating of myoglobins, and intermediate detection line T2 is that creatine kinase is same
The pairing antibody or antigen coating of work enzyme.Nature controlling line C is formed by being coated with mouse IgG antibody.
Each component in this example is assemblied together in this way：In hothouse, 20~25 DEG C of temperature, humidity is less than
40%, bottom plate 5 is taken, the middle part that coated detecting pad 3 is placed on to bottom plate 5 is pasted, and is overlapped by detection line side in detecting pad 3
Boosting pad 7 is pasted, bonding pad 2 is pasted onto the other side of boosting pad 7, and the other side overlap joint of bonding pad 2 pastes loading pad 1；Hemofiltration
Film 6 is covered on loading pad 1；Sample suction pad 4 is overlapped in the nature controlling line side of detecting pad 3.The bottom plate that will finally be posted with cutter
Be cut into the test strips of 3~5mm wide, be packed into plastics get stuck in form detection and block.
The detection method of this example kit is following (dry method loading)：
(1) detection reagent and sample are balanced to room temperature, takes out detection card, keeps flat；
(2) sample to be tested is drawn, is added in clean centrifuge tube, is diluted, is mixed well with Sample dilution；
(3) after the sample after drawing dilution with liquid-transfering gun is added to loading pad 1,15 minutes by sample aperture, according in advance
The standard curve being first arranged in time-resolved fluoroimmunoassay chromatography readout instrument calculates simultaneously the concentration of cTnI, CK-MB and Myo
Show result.
The testing principle of this example detection card is as follows：
After sample is added to sample well, cTnI, CK-MB and Myo antigen and the fluorescent microsphere label that contain in sample
CTnI, CK-MB and Myo antibody combine, and by capillarity, are formed by antibody-antigen immune compound along cellulose nitrate
Plain film layer is analysed to detection line, and high specific and height parent then occur with cTnI, CK-MB and Myo antibody being solidificated in detection line
With the immune response of property, thus immune complex is enriched with or is trapped in the detection of chromatographic material and taken, and unbonded is immune multiple
Conjunction object then continues chromatography and is captured to nature controlling line by sheep anti-mouse igg antibody.After the completion of reaction, detection takes containing for Eu 3+ chelates
Amount and the concentration of target checking matter have certain correspondence.If being free of checking matter in testing sample solution, then detection takes
Just there is no Eu 3+ chelates.It is 103-106 times of conventional fluorescent since the fluorescence decay time of lanthanide ion chelate is long.With
When time-resolved fluorescence instrument measures its fluorescence, after light-pulse generator excitation, it can postpone to measure again for a period of time, at this time
The short-half-life fluorescence of other compositions has decayed, and only exists the specificity fluorescent of Eu3+ markers, by measuring immune response
Afterwards on ELISA test strip band Eu 3+ chelates content, reference standard concentration curve can quantify obtain it is right in sample to be tested
Answer the concentration of antigen.
When after reaction, being analyzed, according to being set in advance in time-resolved fluoroimmunoassay quantitative readout instrument
Standard curve to the concentration of cTnI, CK-MB and Myo carries out that result is calculated and be shown.
Embodiment 2
As shown in Fig. 2, the present embodiment provides a kind of multi objective time resolution for myocardial infarction Quantitative detection is glimmering
Light immune chromatography reagent kit, substantially with embodiment 1, the difference is that, bonding pad 2 is marked without time-resolved fluorescence microballoon
Antibody coating 21, kit still further comprise time-resolved fluorescence microballoon labelled antibody solution.
The detection method of this example kit is following (wet method loading)：
(1) detection reagent and sample are balanced to room temperature, takes out detection card, keeps flat；
(2) sample to be tested is drawn, is sufficiently mixed with time-resolved fluorescence microballoon labelled antibody solution；
(3) it is drawn after mixed liquor is added to loading pad 1,15 minutes by sample aperture with liquid-transfering gun, according to having pre-set
The concentration of cTnI, CK-MB and Myo are carried out that knot is calculated and be shown in the standard curve of time-resolved fluoroimmunoassay quantitative readout instrument
Fruit.
Embodiment 3
As shown in figure 3, the present embodiment provides a kind of multi objective time resolution for myocardial infarction Quantitative detection is glimmering
Light immune chromatography reagent kit, substantially with embodiment 1, the difference is that, detecting pad 3 includes three detections arranged side by side point pad
30,30 ', 30 ", each detection, which divides on pad 30,30 ', 30 ", is respectively formed a detection line and nature controlling line C.
Preparation, detection and the testing principle of this example kit are the same as embodiment 1.
Embodiment 4
As shown in Fig. 4, Fig. 5, Fig. 6, when the multi objective that the present embodiment provides a kind of for myocardial infarction Quantitative detection
Between resolved fluorometric immune chromatography reagent kit, substantially with embodiment 1, unlike, detection card includes that three independent detections divide
Card, hemofiltration film 6, loading pad 1, bonding pad 2, boosting pad 7, detection divide pad 30, sample suction pad 4 and bottom plate 5 to have three respectively, each
Hemofiltration film 6,6 ', 6 ", loading pad 1,1 ', 1 ", bonding pad 2,2 ', 2 ", boosting pad 7,7 ', 7 ", detection divide pad 30,30 ', 30 ", inhale
Sample pad 4,4 ', 4 " and bottom plate 5,5 ', 5 " are assembled into a detection point card, and the detection on each detection point card divides pad 30,30 ', 30 "
On be respectively formed a detection line and a nature controlling line.
Preparation, detection and the testing principle of this example kit are the same as embodiment 1.
Embodiment 5
The present embodiment provides a kind of multi objective time-resolved fluoroimmunoassay chromatographies for myocardial infarction Quantitative detection
Kit, substantially with embodiment 1, the difference is that, without boosting pad 7.Bonding pad 2, which is directly pasted with detecting pad 3, to be connected.This example
The detection of kit and testing principle are the same as embodiment 1.
The utility model is described in detail above, its object is to allow the personage for being familiar with this field technology can be much of that
It solves the content of the utility model and is implemented, the scope of protection of the utility model can not be limited with this, it is all according to this practicality
Equivalent change or modification made by novel Spirit Essence should all cover within the protection scope of the present utility model.
Claims (10)
1. a kind of multi objective time-resolved fluoroimmunoassay for myocardial infarction Quantitative detection chromatographs kit, the kit
Including detection card and selectable fluorescence immunoassay quantitative analysis instrument, the detection card includes loading pad (1), bonding pad (2), has
The detecting pad (3) of detection line and nature controlling line, sample suction pad (4) and bottom plate (5), it is characterised in that：The index includes myocardium myo calcium
Albumen, creatine kinase isozyme and myoglobins, the bonding pad (2) include in conjunction with pad body (20) and being coated on the combination
Time-resolved fluorescence microballoon labelled antibody coating (21) on pad body (20), wherein time-resolved fluorescence microballoon label is anti-
Antibody in body coating (21) is the antibody of the index；The detection line has three, which is separated from each other and divides
It is not the pairing antibody that can be combined with the antibody specificity of the time-resolved fluorescence microballoon labelled antibody coating (21) or anti-
Original shape at coating.
2. a kind of multi objective time-resolved fluoroimmunoassay for myocardial infarction Quantitative detection chromatographs kit, the kit
Including detection card and selectable fluorescence immunoassay quantitative analysis instrument, the detection card includes loading pad (1), bonding pad (2), has
The detecting pad (3) of detection line and nature controlling line, sample suction pad (4) and bottom plate (5), it is characterised in that：The index includes myocardium myo calcium
Albumen, creatine kinase isozyme and myoglobins；The kit further includes time-resolved fluorescence microballoon labelled antibody solution, should
Antibody in time-resolved fluorescence microballoon labelled antibody solution is the antibody of the index；The detection line has three, this three
Detection line is separated from each other and is the pairing antibody that can be specifically bound with the time-resolved fluorescence microballoon labelled antibody respectively
Or the coating that antigen is formed.
3. multi objective time-resolved fluoroimmunoassay according to claim 1 or 2 chromatographs kit, it is characterised in that：It is described
Detection card further includes hemofiltration film (6), which is covered on the loading pad (1)；The loading pad (1), bonding pad
(2), detecting pad (3) and sample suction pad (4) is interlaced successively is pasted onto on bottom plate (5).
4. multi objective time-resolved fluoroimmunoassay according to claim 1 or 2 chromatographs kit, it is characterised in that：It is described
Detection card further includes boosting pad (7), and detection card is by the loading pad (1), bonding pad (2), boosting pad (7), detecting pad
(3) and sample suction pad (4) interlaced be pasted onto on the bottom plate (5) is constituted successively, the material of the boosting pad (7) is glass fibers
The plain film of dimension or non-woven fabrics.
5. multi objective time-resolved fluoroimmunoassay according to claim 1 or 2 chromatographs kit, it is characterised in that：It is described
Detection card further includes hemofiltration film (6) and boosting pad (7), and the hemofiltration film (6) is covered on the loading pad (1), the loading
Pad (1), bonding pad (2), boosting pad (7), detecting pad (3) and sample suction pad (4) is interlaced successively is pasted onto on bottom plate (5), institute
The material for stating boosting pad (7) is glass fibre element film or non-woven fabrics.
6. multi objective time-resolved fluoroimmunoassay according to claim 1 or 2 chromatographs kit, it is characterised in that：It is described
Detecting pad (3) only has one, and the three detections line and nature controlling line are mutually parallel and along the length directions of the detecting pad (3)
It is successively set on the detecting pad (3).
7. multi objective time-resolved fluoroimmunoassay according to claim 6 chromatographs kit, it is characterised in that：The Quality Control
Line is located at end of the detecting pad (3) close to the sample suction pad (4)；Close to the inspection of bonding pad (2) in the three detections line
Survey line is the pairing antibody or antigen coating of cardiac troponin, and the detection line close to nature controlling line is the pairing antibody of myoglobins
Or antigen coating, intermediate detection line are the pairing antibody or antigen coating of creatine kinase isozyme.
8. multi objective time-resolved fluoroimmunoassay according to claim 1 or 2 chromatographs kit, it is characterised in that：It is described
Detecting pad (3) includes three detection point pads (30,30 ', 30 "), and the three detections line is respectively distributed to three detections
Divide on pad (30,30 ', 30 ").
9. multi objective time-resolved fluoroimmunoassay according to claim 8 chromatographs kit, it is characterised in that：The detection
Card includes three independent detection point cards, the loading pad (1,1 ', 1 "), bonding pad (2,2 ', 2 "), sample suction pad (4,4 ', 4 ")
Respectively have three with bottom plate (5,5 ', 5 "), each loading pad (1,1 ', 1 "), bonding pad (2,2 ', 2 "), detection point pad (30,
30 ', 30 "), sample suction pad (4,4 ', 4 ") and bottom plate (5,5 ', 5 ") are assembled into the detection described in one point card.
10. multi objective time-resolved fluoroimmunoassay according to claim 1 or 2 chromatographs kit, it is characterised in that：It is described
Nature controlling line is formed by being coated with sheep anti-mouse igg, goat-anti chicken IgY or goat anti-rabbit igg antibody, and the detecting pad (3) is cellulose nitrate
Plain film and the porous spline structure film for being 5~12um of aperture；The loading pad (1), bonding pad (2) material be glass fibre element film
Or non-woven fabrics, the material of the sample suction pad (4) is absorbent filter.
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| CN201820626259.0U CN208060389U (en) | 2018-04-28 | 2018-04-28 | A kind of multi objective time-resolved fluoroimmunoassay for myocardial infarction Quantitative detection chromatographs kit |
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| CN201820626259.0U CN208060389U (en) | 2018-04-28 | 2018-04-28 | A kind of multi objective time-resolved fluoroimmunoassay for myocardial infarction Quantitative detection chromatographs kit |
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| CN111896745A (en) * | 2020-07-29 | 2020-11-06 | 泰安京泰生物技术有限公司 | Kit for jointly detecting cardiac marker by colloidal gold method and preparation method and application thereof |
| CN111896745B (en) * | 2020-07-29 | 2021-05-25 | 泰安京泰生物技术有限公司 | Kit for jointly detecting cardiac marker by colloidal gold method and preparation method and application thereof |
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