CN109342741B - Quick-insertion type imprinting film clamping device - Google Patents
Quick-insertion type imprinting film clamping device Download PDFInfo
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- CN109342741B CN109342741B CN201811441575.1A CN201811441575A CN109342741B CN 109342741 B CN109342741 B CN 109342741B CN 201811441575 A CN201811441575 A CN 201811441575A CN 109342741 B CN109342741 B CN 109342741B
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- 238000003780 insertion Methods 0.000 title claims abstract description 11
- 238000003825 pressing Methods 0.000 claims abstract description 55
- 239000000758 substrate Substances 0.000 claims abstract description 52
- 239000012528 membrane Substances 0.000 claims abstract description 18
- 238000001746 injection moulding Methods 0.000 claims abstract description 6
- 238000003119 immunoblot Methods 0.000 claims description 14
- 238000004458 analytical method Methods 0.000 claims description 11
- 230000037431 insertion Effects 0.000 claims 2
- 239000012085 test solution Substances 0.000 abstract description 19
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 238000001179 sorption measurement Methods 0.000 abstract description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 description 9
- 238000010586 diagram Methods 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 5
- 238000000034 method Methods 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 230000035484 reaction time Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000001262 western blot Methods 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 238000000376 autoradiography Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000002998 immunogenetic effect Effects 0.000 description 1
- 230000036046 immunoreaction Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000000696 magnetic material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 235000004252 protein component Nutrition 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
- G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
Abstract
The invention provides a quick-insertion type imprinting film clamping device, which comprises: the auxiliary supporting assembly comprises a bottom plate and a push plate, the clamping assembly comprises a clamping main plate, an upper pressing plate and a clamping piece, and a regulation groove for inserting the clamping piece is formed between the upper pressing plate and the clamping main plate; the clamping piece comprises a substrate part, a flexible connecting part and a pressing part which are sequentially connected, the imprinting film is integrally and stably adsorbed on the surface of the substrate part by the adsorption force of a water film, the upper end of the imprinting film is clamped between the substrate part and the pressing part, the part of the imprinting film, which enters the test solution, can be separated from the substrate part to fully react with the test solution, and the imprinting film can be integrally and stably adsorbed on the surface of the substrate part after being separated from the test solution. The clamping piece is integrally molded by injection molding, so that the manufacturing cost of the clamping piece is greatly saved; after the membrane is placed, only the pushing plate is pushed to push one end of the clamping piece into the regulation groove, the pressing part of the clamping piece is pressed and stably clamps the upper end of the imprinting membrane, so that the operation is quick and convenient, and the experimental efficiency is improved.
Description
Technical Field
The invention relates to an experiment auxiliary device, in particular to a fast-inserting type imprinting film clamping device.
Background
Immunoblotting is also known as Western Blot (Western Blot). The method is a commonly used experimental method in biochemistry and immunogenetics, which is to transfer the total protein of cells or tissues after electrophoresis separation from gel to a solid carrier blotting membrane, take the protein or polypeptide on the blotting membrane as antigen, react with a corresponding antibody, react with an enzyme or isotope labeled secondary antibody, and finally detect the protein component expressed by the specific target gene after electrophoresis separation through substrate color development or autoradiography. Throughout the immunoreaction experiment, multiple wash-incubation-wash operations with multiple reagents were required for the blotting membrane.
At present, in a laboratory, a sample is generally placed in a reaction vessel, a test solution is added into the reaction vessel in the experimental process, the test solution is sucked out and cleaned through a liquid suction gun after a single reaction is finished, and a large amount of unconsumed antibodies are contained in the discarded primary and secondary antibody test solutions, so that serious waste of the primary and secondary antibodies is caused, and the primary and secondary antibodies are relatively expensive, so that higher experimental cost is caused. Therefore, there is a need in the market for an immunoblotting analysis device with a blotting membrane capable of actively moving between each test solution, such that the first-antibody and the second-antibody can be reused, but how to realize the rapid and stable clamping of the blotting membrane is a primary topic to be solved.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention aims to provide a quick-insertion type imprinting film clamping device.
In order to achieve the above purpose, the technical scheme adopted by the invention for solving the technical problems is as follows: a quick-insertion blotting membrane clamping device, comprising:
an auxiliary support assembly comprising a base plate and a push plate, wherein the upper surface of the base plate comprises a first support surface and a second support surface, and the push plate is arranged on the first support surface of the base plate and can translate back and forth along the first support surface;
the clamping assembly comprises a clamping main plate, an upper pressing plate and a clamping piece, wherein the clamping main plate is arranged on a second supporting surface of the bottom plate, the upper pressing plate is fixed on the clamping main plate, and a regulation groove for inserting the clamping piece is formed between the upper pressing plate and the clamping main plate; the clamping piece comprises a substrate part, a flexible connecting part and a pressing part which are sequentially connected, the pressing part can be contacted with and separated from the substrate part around the flexible connecting part, the pressing part is provided with an arc-shaped curved surface section for generating tension force, the clamping piece is integrally formed through an injection molding process, the upper end of a imprinting film is clamped between the substrate part of the clamping piece and the pressing part, and the lower bottom surfaces of the two ends of the substrate part are respectively attached to the upper surface of the push plate and the surface of the clamping main board.
Compared with the prior art, the imprinting film is integrally and stably adsorbed on the surface of the substrate part by the adsorption force of the water film, the upper end of the imprinting film is clamped between the substrate part and the pressing part, the part of the imprinting film entering the test solution can be separated from the substrate part to fully react with the test solution, and the imprinting film is integrally and stably adsorbed on the surface of the substrate part after being separated from the test solution; the clamping piece is integrally molded by injection molding, so that the manufacturing cost of the clamping piece is greatly saved; when the film is placed, two ends of the bottom surface of the substrate part of the clamping piece are respectively attached to the upper surface of the push plate and the surface of the clamping main plate which are positioned on the same plane, the imprinting film is not deformed, only the push plate is pushed to push one end of the clamping piece into the regulation groove, the pressing part of the clamping piece is pressed to stably clamp the imprinting film, the operation is quicker and more convenient, and the experimental efficiency is improved; after the imprinting membrane is clamped, the clamping assembly is moved out from the auxiliary supporting assembly and hung on a moving plate of the immunoblotting analysis equipment, and the immunoblotting analysis equipment moves among all the test solution tanks according to the reaction time, so that the multi-frequency cleaning-incubation-cleaning is performed, the waste of antibody test solutions is reduced, and the experimental cost is saved.
Further, positioning blocks are arranged at two ends of the clamping main board, positioning grooves matched with the positioning blocks are formed in the bottom plate, positioning columns extending outwards are arranged on the lower surface of the positioning blocks, and positioning holes matched with the positioning columns are formed in the bottoms of the positioning grooves in the bottom plate.
By adopting the preferable scheme, the position accuracy of the clamping assembly on the auxiliary supporting assembly is improved, the clamping assembly is convenient to take and place, and the experimental operation efficiency is improved.
Further, a strip-shaped first guide groove is formed in the substrate part of the clamping piece, and a guide rib corresponding to the first guide groove is arranged on the clamping main board; the upper pressing plate is provided with a second guide groove, and the pressing part of the clamping piece is provided with a guide post corresponding to the second guide groove.
By adopting the preferable scheme, the substrate part is stably translated in the regulation groove through the cooperation of the first guide groove and the guide rib; through the cooperation of second guide way and guide pillar, make the pressure hold portion stable translation in the regulation inslot, ensure that the grip tab will print the stable centre gripping of membrane.
Further, a limiting column extending towards the substrate part is arranged on the surface, opposite to the substrate part, of the pressing part of the clamping piece, and a limiting hole matched with the limiting column is formed in the substrate part.
By adopting the preferable scheme, when the pressing part presses downwards, the limit column is matched with the limit hole, so that the position of the head end of the pressing part on the substrate part is ensured to be stable, and the imprinting film is prevented from moving.
Further, a hooking hole which is sunken towards the bottom surface is formed in the root of the second guide groove of the upper pressing plate, and the guide pillar enters the hooking hole when the clamping piece is inserted into the root of the regulation groove.
By adopting the preferable scheme, after the clamping piece is inserted in place, the guide pillar enters the hooking hole, so that the clamping piece can be prevented from easily falling out, and the clamping stability of the imprinting film is improved.
Further, the hooking hole penetrates through the upper surface of the upper pressing plate, and the opening of the hooking hole on the upper surface of the upper pressing plate forms an inward concave conical surface.
By adopting the preferable scheme, when the clamping piece and the imprinting film need to be taken out after the immunoblotting experiment is finished, the clamping piece can be easily pulled out of the regulation groove only by downwards pressing the guide post from the upper surface of the upper pressing plate; the concave conical surface is convenient for operators to directly press with fingers, and the operation convenience is improved.
Further, a magnetic body is embedded on the joint surface of the clamping main board and the bottom board.
By adopting the preferable scheme, a certain suction force is kept between the clamping main board and the bottom board, so that the clamping main board and the bottom board are prevented from being shifted after slight stress; after the imprinting film is clamped, the clamping assembly can be taken out with a little force, so that the operation convenience is improved.
Further, a locating pin is arranged on the push plate, and a semicircular opening matched with the locating pin is arranged at the head of the substrate part of the clamping piece.
By adopting the preferable scheme, one end of the substrate part of the clamping piece is matched with the guide rib through the first guide groove, and the other end of the substrate part of the clamping piece is matched with the positioning pin through the semicircular opening, so that the stability of the clamping piece in the moving process is improved.
Further, the clamping main board is further provided with a hanging lug for hanging the clamping assembly on the immunoblotting analysis equipment, and the hanging lug is provided with a mounting hole.
By adopting the preferable scheme, the convenience of the clamping assembly to be installed on the immunoblotting analysis equipment is improved.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of an embodiment of the present invention;
FIG. 2 is a schematic diagram of the structure of an embodiment of the present invention;
FIG. 3 is a schematic view of another embodiment of the auxiliary support assembly of the present invention;
FIG. 4 is a schematic view showing the structure of the combination of the clamping main plate and the upper pressing plate according to the present invention;
FIG. 5 is a schematic diagram illustrating the structure of an embodiment of a clamping motherboard according to the present invention;
FIG. 6 is a schematic view of the structure of an embodiment of the upper platen of the present invention;
FIG. 7 is a schematic view of another embodiment of an upper platen of the present invention;
FIG. 8 is a schematic view of the structure of one embodiment of the clamping plate of the present invention;
FIG. 9 is a schematic view of the structure of one embodiment of the clamping plate of the present invention;
FIG. 10 is a schematic view of the structure of one embodiment of the clamping plate of the present invention;
fig. 11 is a schematic structural diagram of an immunoblot analysis apparatus.
Names of the corresponding parts indicated by numerals and letters in the drawings:
1-an auxiliary support assembly; 11-a bottom plate; 111-a first bearing surface; 112-a second bearing surface; 113-positioning grooves; 114-positioning holes; 12-pushing plate; 121-a locating pin; 2-a clamping assembly; 21-clamping the main board; 211-regulation groove; 212-positioning blocks; 213-positioning columns; 214-guiding ribs; 215-mounting holes; 22-upper press plate; 221-a second guide groove; 222-hooking holes; 223-concave conical surface; 23-clamping pieces; 231-substrate portion; 232-a flexible connection; 233-a press-holding part; 234—a first guide slot; 235-guide posts; 236-a limit column; 237-limiting holes; 238-semicircular opening; 3-a forward and backward movement mechanism; 4-an up-down moving mechanism; 5-moving plate; 6-a test solution box.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
As shown in fig. 1-10, a quick-insertion type blotting membrane clamping device includes:
a quick-insertion blotting membrane clamping device, comprising:
the auxiliary supporting assembly 1 comprises a bottom plate 11 and a push plate 12, wherein the upper surface of the bottom plate 11 comprises a first supporting surface 111 and a second supporting surface 112, and the push plate 12 is arranged on the first supporting surface 111 of the bottom plate 11 and can translate back and forth along the first supporting surface 111;
the clamping assembly 2 comprises a clamping main plate 21, an upper pressing plate 22 and a clamping piece 23, wherein the clamping main plate 21 is arranged on the second supporting surface 112 of the bottom plate 11, the upper pressing plate 22 is fixed on the clamping main plate 21, and a regulation groove 211 for inserting the clamping piece 23 is formed between the upper pressing plate 22 and the clamping main plate 21; the clamping piece 23 comprises a substrate part 231, a flexible connection part 232 and a pressing part 233 which are sequentially connected, the pressing part 233 can be contacted with and separated from the substrate part 231 around the flexible connection part 232, the pressing part 233 is provided with an arc-shaped curved surface section for generating tension, the clamping piece 23 is integrally formed through an injection molding process, the upper end of a imprinting film is clamped between the substrate part 231 and the pressing part 233 of the clamping piece 23, and the lower bottom surfaces of the two ends of the substrate part 231 are respectively attached to the upper surface of the push plate 12 and the surface of the clamping main plate 21.
The beneficial effects of adopting above-mentioned technical scheme are: the imprinting film is integrally and stably adsorbed on the surface of the substrate 231 by the adsorption force of the water film, the upper end of the imprinting film is clamped between the substrate 231 and the pressing part 233, the part of the imprinting film entering the test solution can be separated from the substrate to fully react with the test solution, and the imprinting film is integrally and stably adsorbed on the surface of the substrate after being separated from the test solution; the clamping piece 23 is integrally molded by injection molding, so that the manufacturing cost of the clamping piece is greatly saved; when the film is put, the two ends of the lower bottom surface of the substrate 231 of the clamping piece are respectively attached to the upper surface of the push plate and the surface of the clamping main plate which are positioned on the same plane, so that the deformation of the imprinting film is not caused, the push plate 12 is pushed to push one end of the clamping piece 23 into the regulation groove, the imprinting film is stably clamped by the pressing part of the clamping piece under pressure, the state schematic diagram before the clamping piece 23 is pushed in is shown in fig. 1, the state schematic diagram after the clamping piece 23 is pushed in is shown in fig. 2, the operation is rapid and convenient, and the experimental efficiency is improved. As shown in fig. 11, an immunoblotting analysis device comprises a front-back moving mechanism 3, an up-down moving mechanism 4, a moving plate 5 and a plurality of test solution boxes 6, wherein after the blotting membrane is clamped, a clamping assembly 2 is moved out of an auxiliary supporting assembly 1 and stably hung on the moving plate 5 of the immunoblotting analysis device, and the blotting membrane on the clamping assembly is moved between the test solution boxes by the cooperation of the front-back moving mechanism 3 and the up-down moving mechanism 4 according to the reaction time, so that the cleaning-incubation-cleaning of multiple frequencies is performed, the waste of antibody test solutions is reduced, and the experimental cost is saved.
As shown in fig. 3 and 5, in other embodiments of the present invention, positioning blocks 212 are disposed at two ends of the clamping main board 21, positioning slots 113 matched with the positioning blocks 212 are disposed on the bottom board 11, positioning columns 213 extending outwards are disposed on the lower surface of the positioning blocks 212, and positioning holes 114 matched with the positioning columns 213 are disposed at the bottom of the positioning slots 113 on the bottom board. The beneficial effects of adopting above-mentioned technical scheme are: the position accuracy of the clamping assembly on the auxiliary supporting assembly is improved, the clamping assembly is quite convenient to take and place, and the experimental operation efficiency is improved.
As shown in fig. 5, 6, 9 and 10, in other embodiments of the present invention, the substrate portion 231 of the holding piece 23 is provided with a first elongated guide groove 234, and the holding main plate 21 is provided with guide ribs 214 corresponding to the first guide groove 234; the upper pressing plate 22 is provided with a second guiding groove 221, and the pressing part 233 of the clamping piece is provided with a guide pillar 235 corresponding to the second guiding groove 221. The beneficial effects of adopting above-mentioned technical scheme are: the substrate part stably translates in the regulation groove by the cooperation of the first guide groove 234 and the guide rib 214; through the cooperation of the second guide groove 221 and the guide pillar 235, the pressing part stably translates in the regulation groove, and the clamping piece is ensured to stably clamp the imprinting film.
As shown in fig. 8 and 10, in other embodiments of the present invention, a surface of the holding portion 233 of the holding piece opposite to the substrate portion is provided with a stopper post 236 extending toward the substrate portion, and the substrate portion 231 is provided with a stopper hole 237 matching the stopper post 236. The beneficial effects of adopting above-mentioned technical scheme are: when the pressing part presses downwards, the limiting column is matched with the limiting hole, so that the position of the head end of the pressing part on the substrate part is ensured to be stable, and the imprinting film is prevented from moving.
In other embodiments of the present invention, as shown in fig. 7, the root of the second guiding groove 221 of the upper pressing plate 22 is provided with a hooking hole 222 recessed toward the bottom surface, and the guide post 235 enters the hooking hole 222 when the clamping piece is inserted into the root of the regulating groove. The beneficial effects of adopting above-mentioned technical scheme are: after the clamping piece is inserted in place, the guide post enters the hooking hole, so that the clamping piece can be prevented from easily falling out, and the clamping stability of the imprinting film is improved.
In other embodiments of the present invention, as shown in fig. 7, the hooking hole 222 is formed through the upper surface of the upper platen 22, and the hooking hole 222 is formed as a concave conical surface 223 at the mouth of the upper surface of the upper platen 22. The beneficial effects of adopting above-mentioned technical scheme are: after the immunoblotting experiment is finished, when the clamping piece and the blotting membrane are required to be taken out, the clamping piece can be easily pulled out of the regulation groove only by downwards pressing the guide post from the upper surface of the upper pressing plate; the concave conical surface is convenient for operators to directly press with fingers, and the operation convenience is improved.
In other embodiments of the present invention, a magnetic material is embedded in the contact surface between the clamping main board 21 and the bottom board 11. The beneficial effects of adopting above-mentioned technical scheme are: a certain suction force is kept between the clamping main board and the bottom board, so that the clamping main board and the bottom board are prevented from being shifted after slight stress; after the imprinting film is clamped, the clamping assembly can be taken out with a little force, so that the operation convenience is improved.
In other embodiments of the present invention, as shown in fig. 3 and 9, the pusher plate 12 is provided with a positioning pin 121, and the head of the clamping plate substrate 231 is provided with a semicircular opening 238 matching the positioning pin 121. The beneficial effects of adopting above-mentioned technical scheme are: one end of the substrate part of the clamping piece is matched with the guide rib 214 through the first guide groove 234, and the other end of the substrate part of the clamping piece is matched with the positioning pin 121 through the semicircular opening 238, so that the stability of the clamping piece in the moving process is improved.
In other embodiments of the present invention, as shown in fig. 5, a suspension lug for suspending the clamping assembly 2 to the immunoblot analysis apparatus is further provided on the clamping main plate 21, and a mounting hole 215 is provided on the suspension lug. The beneficial effects of adopting above-mentioned technical scheme are: the convenience of the clamping assembly to be installed on the immunoblotting analysis equipment is improved.
The above embodiments are only for illustrating the technical concept and features of the present invention, and are intended to enable those skilled in the art to understand the content of the present invention and implement the same, but not limit the scope of the present invention, and all equivalent changes or modifications made according to the spirit of the present invention should be included in the scope of the present invention.
Claims (4)
1. Quick-insertion type imprinting film clamping device is characterized by comprising:
an auxiliary support assembly comprising a base plate and a push plate, wherein the upper surface of the base plate comprises a first support surface and a second support surface, and the push plate is arranged on the first support surface of the base plate and can translate back and forth along the first support surface;
the clamping assembly comprises a clamping main plate, an upper pressing plate and a clamping piece, wherein the clamping main plate is arranged on a second supporting surface of the bottom plate, the upper pressing plate is fixed on the clamping main plate, a regulation groove for inserting the clamping piece is formed between the upper pressing plate and the clamping main plate, the clamping piece comprises a substrate part, a flexible connecting part and a pressing part which are sequentially connected, the pressing part can be contacted with and separated from the substrate part around the flexible connecting part, the pressing part is provided with an arc-shaped curved surface section for generating tension force, the clamping piece is integrally formed through an injection molding process, a imprinting film is integrally adsorbed on the surface of the substrate part, and the upper end of the imprinting film is clamped between the substrate part and the pressing part;
positioning blocks are arranged at two ends of the clamping main board, positioning grooves matched with the positioning blocks are formed in the bottom plate, positioning columns extending outwards are arranged on the lower surface of the positioning blocks, and positioning holes matched with the positioning columns are formed in the bottoms of the positioning grooves on the bottom plate;
the substrate part of the clamping piece is provided with a strip-shaped first guide groove, and the clamping main board is provided with guide ribs corresponding to the first guide groove; the upper pressing plate is provided with a second guide groove, and the pressing part of the clamping piece is provided with a guide post corresponding to the second guide groove;
a limiting column extending towards the substrate part is arranged on the surface, opposite to the substrate part, of the pressing part of the clamping piece, and a limiting hole matched with the limiting column is formed in the substrate part;
the root of the second guide groove of the upper pressing plate is provided with a hooking hole which is concave towards the bottom surface, and when the clamping piece is inserted into the root of the regulation groove, the guide pillar enters the hooking hole;
the hooking hole penetrates through the upper surface of the upper pressing plate, and the opening of the upper surface of the upper pressing plate forms an inward concave conical surface.
2. The rapid insertion type blotting membrane clamping device according to claim 1, wherein a magnetic body is embedded on the joint surface of the clamping main board and the bottom board.
3. The rapid insertion type blotting membrane clamping device according to claim 2, wherein the push plate is provided with a positioning pin, and the head of the substrate part of the clamping piece is provided with a semicircular opening matched with the positioning pin.
4. A quick-insertion blotting membrane clamping device according to claim 3, wherein the clamping main board is further provided with a hanging lug for hanging the clamping assembly on the immunoblotting analysis equipment, and the hanging lug is provided with a mounting hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811441575.1A CN109342741B (en) | 2018-11-29 | 2018-11-29 | Quick-insertion type imprinting film clamping device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811441575.1A CN109342741B (en) | 2018-11-29 | 2018-11-29 | Quick-insertion type imprinting film clamping device |
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| Publication Number | Publication Date |
|---|---|
| CN109342741A CN109342741A (en) | 2019-02-15 |
| CN109342741B true CN109342741B (en) | 2024-02-23 |
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| CN201811441575.1A Active CN109342741B (en) | 2018-11-29 | 2018-11-29 | Quick-insertion type imprinting film clamping device |
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| WO2016058338A1 (en) * | 2014-10-14 | 2016-04-21 | 杭州浙大迪迅生物基因工程有限公司 | Western blot test board reaction device |
| CN204964522U (en) * | 2015-09-28 | 2016-01-13 | 中国人民解放军第四军医大学 | Protein immunoblotting antibody board |
| CN205620419U (en) * | 2016-02-16 | 2016-10-05 | 张新化 | Western blotting determine module |
| CN107525841A (en) * | 2017-06-02 | 2017-12-29 | 吴荣荣 | A kind of novel protein trace instrument and its test method |
| CN207408417U (en) * | 2017-10-31 | 2018-05-25 | 长江大学 | A kind of Western blotting exposure box |
| CN209215403U (en) * | 2018-11-29 | 2019-08-06 | 莫纳(连云港)生物科技有限公司 | A kind of push-in blotting membrane clamping device |
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| CN109342741A (en) | 2019-02-15 |
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