CN217910484U - Substrate surface modification device for gene sequencing chip - Google Patents
Substrate surface modification device for gene sequencing chip Download PDFInfo
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- CN217910484U CN217910484U CN202221389905.9U CN202221389905U CN217910484U CN 217910484 U CN217910484 U CN 217910484U CN 202221389905 U CN202221389905 U CN 202221389905U CN 217910484 U CN217910484 U CN 217910484U
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- gene sequencing
- sequencing chip
- pressing plate
- substrate
- fixing clamp
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Abstract
The utility model provides a basement surface modification device for gene sequencing chip relates to the chemical reaction device field, include: the device comprises an upper pressing plate, a gene sequencing chip substrate, a lower pressing plate and a fixing clamp; the gene sequencing chip substrate is clamped between the upper pressing plate and the lower pressing plate; the gene sequencing chip substrate is hermetically connected with the upper pressing plate, and a flow channel is formed between the gene sequencing chip substrate and the upper pressing plate; the gene sequencing chip substrate, the upper pressing plate and the lower pressing plate are all fixed in the fixing clamp; a channel is arranged in the fixing clamp and communicated with the outside, and the channel is communicated with the flow channel. The utility model discloses a cooperation of top board, gene sequencing chip basement and holding down plate three forms the closed flow way, helps isolated air pollution, helps convenient operation, split facility to help improving the efficiency of experiment.
Description
Technical Field
The utility model relates to a chemical reaction device field specifically relates to a basement surface modification device for gene sequencing chip.
Background
With the development of molecular biology and the more and more intensive research of people on life science, gene testing chips are widely applied to laboratories, biochemical experiments have higher and higher requirements on devices related to chemical reactions, and gene sequencing chip substrate surface modification devices are continuously perfected.
The prior Chinese patent with publication number CN214439153U discloses a microfluidic chip surface modification device, which comprises a bottom support, a PDMS cover plate and an upper cover, wherein a connecting device is correspondingly arranged between the bottom support and the upper cover, the PDMS cover plate is clamped between the bottom support and the upper cover, a groove is arranged on one side surface of the PDMS cover plate, which is in contact with the microfluidic chip, a sample inlet and a sample outlet for the modification solution to enter and exit are correspondingly arranged on the PDMS cover plate and the upper cover, and the sample inlet and the sample outlet are both communicated with the groove.
The inventor thinks that in order to detect gene information, biochemical reaction sequencing reagents are generally injected into a gene sequencing chip in sequence to carry out necessary biochemical reaction in the sequencing process in the prior art, the preparation of the gene sequencing chip substrate has extremely high requirement on cleanliness, and when the gene sequencing chip is prepared in a laboratory, the experimental conditions are not ideal and are easily influenced by air pollutants. Therefore, it is a technical problem to be solved by those skilled in the art to design a substrate surface modification device for gene sequencing chip preparation, which can isolate air pollution, has a simple structure and is convenient to operate.
SUMMERY OF THE UTILITY MODEL
To overcome the drawbacks of the prior art, the present invention provides a substrate surface modification device for gene sequencing chip.
According to the utility model provides a pair of a basement surface modification device for gene sequencing chip, include: the device comprises an upper pressing plate, a gene sequencing chip substrate, a lower pressing plate and a fixing clamp; the gene sequencing chip substrate is clamped between the upper pressing plate and the lower pressing plate; the gene sequencing chip substrate is hermetically connected with the upper pressing plate, and a flow channel is formed between the gene sequencing chip substrate and the upper pressing plate; the gene sequencing chip substrate, the upper pressing plate and the lower pressing plate are all fixed in the fixing clamp; a channel is arranged in the fixing clamp and communicated with the outside, and the channel is communicated with the flow channel.
Preferably, the lower surface of the upper pressure plate is provided with a runner groove.
Preferably, both ends of the upper pressure plate are respectively provided with a liquid inlet and a liquid outlet, and the liquid inlet and the liquid outlet are both communicated with the runner groove.
Preferably, the both ends of mounting fixture upper surface are provided with the inlet connector respectively and go out the liquid joint, the inlet connector with the inlet corresponds the setting and communicates, go out the liquid joint with the liquid outlet corresponds the setting and communicates.
Preferably, the liquid inlet joint is connected with a liquid inlet conduit, and the liquid outlet joint is connected with a liquid outlet conduit.
Preferably, both sides of the bottom of the upper pressure plate are provided with splitting notches.
Preferably, one side of the fixing clamp is provided with an inward-recessed fixing groove, and a limiting step is arranged in the fixing groove.
Preferably, a fastening screw is arranged on the lower surface of the fixing clamp.
Preferably, the upper pressure plate comprises a transparent anti-corrosion rigid plate, and the lower surface of the upper pressure plate is smooth and flat; the lower pressing plate comprises a transparent rigid anticorrosive plate, and the upper surface of the transparent rigid anticorrosive plate is smooth and flat; the fixing clamp comprises an anti-corrosion rigid clamp.
Preferably, a base is arranged at the bottom of the fixing clamp.
Compared with the prior art, the utility model discloses following beneficial effect has:
1. the utility model discloses a cooperation of top board, gene sequencing chip basement and holding down plate three forms and seals the runner, helps isolated air pollution, helps convenient operation, split facility to help improving the efficiency of experiment.
2. The utility model discloses an it realizes the sealed of liquid in the runner to exert pressure to top board and holding down plate, helps the simplified operation, through set up the fixed slot on mounting fixture, helps the split convenient.
3. The utility model discloses a top board lower surface is provided with the runner groove, and the interior volume of runner is little, helps saving reactant.
Drawings
Other features, objects and advantages of the invention will become more apparent upon reading of the detailed description of non-limiting embodiments with reference to the following drawings:
FIG. 1 is a schematic diagram showing the overall structure of a substrate surface modification apparatus for a gene sequencing chip according to the present invention;
fig. 2 is a schematic view of the overall structure of the upper platen according to the present invention;
FIG. 3 is a perspective view showing the overall structure of the apparatus for modifying the surface of a substrate for a gene sequencing chip according to the present invention;
fig. 4 is a schematic view of the present invention mainly showing the upward state of the opening of the fixing groove.
Reference numerals:
Go out liquid joint 4 mounting fixture 5 top board 6
Gene sequencing chip basement 7 holding down plate 8 holding screw 9
The liquid inlet 201 of the base 10 is provided with a split notch 202
Detailed Description
The present invention will be described in detail with reference to the following embodiments. The following examples will assist those skilled in the art in further understanding the present invention, but are not intended to limit the invention in any way. It should be noted that various changes and modifications can be made by one skilled in the art without departing from the spirit of the invention. These all belong to the protection scope of the present invention.
As shown in fig. 1, the present invention provides a substrate surface modification apparatus for gene sequencing chip, comprising: an upper pressing plate 6, a gene sequencing chip substrate 7, a lower pressing plate 8 and a fixing clamp 5; the gene sequencing chip substrate 7 is clamped between the upper pressing plate 6 and the lower pressing plate 8; the gene sequencing chip substrate 7 is hermetically connected with the upper pressing plate 6, and a flow channel is formed between the gene sequencing chip substrate and the upper pressing plate; the gene sequencing chip substrate 7, the upper pressing plate 6 and the lower pressing plate 8 are all fixed in the fixing clamp 5; a channel is arranged in the fixing clamp 5, the channel is communicated with the outside, and the channel is communicated with the flow channel.
Two ends of the upper pressure plate 6 are respectively provided with an overflowing hole, the two overflowing holes are respectively a liquid inlet 201 and a liquid outlet 203, the lower surface of the upper pressure plate 6 is provided with a runner groove 204, and the liquid inlet 201 and the liquid outlet 203 are both communicated with the runner groove 204. The middle of the lower surface of the upper pressure plate 6 is formed with a channel groove 204 by machining technology, and the inner wall of the channel groove 204 is flame polished to store the necessary volume of biochemical reaction reagent.
The gene sequencing chip substrate 7 is clamped between the upper pressing plate 6 and the lower pressing plate 8 and is fixed in the fixing clamp 5, the gene sequencing chip substrate 7 is in rigid contact with the upper pressing plate 6, and a closed flow channel is formed between the flow channel groove 204 of the upper pressing plate 6 and the gene sequencing chip substrate 7 under the action of pressure so as to ensure the sealing property and be used for biochemical reaction reagent circulation. The surface modification chemical reaction of the gene sequencing chip substrate 7 only occurs in the flow channel, the volume in the flow channel is small, and the reaction reagent can be fully saved.
The left end and the right end of the upper surface of the fixing clamp 5 are respectively provided with an overflowing joint, the two overflowing joints are respectively a liquid inlet joint 3 and a liquid outlet joint 4, the liquid inlet joint 3 is correspondingly arranged and communicated with the liquid inlet 201, and the liquid outlet joint 4 is correspondingly arranged and communicated with the liquid outlet 203. The liquid inlet joint 3 is connected with a liquid inlet conduit 1, and the liquid outlet joint 4 is connected with a liquid outlet conduit 2. The guide pipe is made of Teflon pipe and can bear the corrosiveness of solvents such as concentrated sulfuric acid.
The biochemical reaction reagent enters the liquid inlet 201 from the liquid inlet conduit 1 through the liquid inlet joint 3, and then flows into the flow channel groove 204, and the surface modification chemical reaction of the gene sequencing chip substrate 7 occurs in the flow channel. After the reaction is finished, the reaction liquid flows out from the liquid outlet 203 through the liquid outlet joint 4 and the liquid outlet conduit 2.
Furthermore, the overflowing connector is installed through threads, a hole of the overflowing connector is aligned with an overflowing hole of the upper pressure plate 6, the overflowing connector is screwed to achieve sealing with the upper pressure plate 6, and meanwhile downward pressure can be formed on the upper pressure plate 6.
Both sides all are provided with split breach 202 around top board 6 bottom, use tweezers to prize out gene sequencing chip basement 7 after the reaction of being convenient for, have improved the convenience of splitting after the operation.
The lower surface of the fixing clamp 5 is provided with a set screw 9. By screwing the fastening screw 9, upward pressure can be formed on the lower pressing plate 8, and the lower pressing plate can be firmly attached to the gene sequencing chip substrate 7.
The front side of the fixing clamp 5 is provided with an inward-concave fixing groove for fixing a combination of an upper pressing plate 6, a gene sequencing chip substrate 7 and a lower pressing plate 8. A limiting step 301 is arranged in the fixing groove to ensure that the upper pressure plate 6 cannot move when being pressed.
Two overflow connectors are arranged on the fixing clamp 5, downward pressure can be applied to the upper pressure plate 6 through aligning and screwing, three fastening screws 9 are arranged below the overflow connectors, upward pressure can be formed on the lower pressure plate 8 through screwing the fastening screws 9, and accordingly the flow channel sealing performance can be guaranteed.
The bottom of the fixing clamp 5 is provided with a base for stably placing the device.
The upper press plate 6 comprises a transparent anti-corrosion rigid plate, and the lower surface of the upper press plate is smooth and flat. The lower press plate 8 comprises a transparent rigid corrosion-resistant plate, and the upper surface of the lower press plate is smooth and flat. Preferably, the material is quartz glass. The fixing clamp 5 is an anti-corrosion rigid clamp.
Because the lower surface of the upper pressing plate 6, the upper surface of the lower pressing plate 8 and the upper and lower surfaces of the gene sequencing chip substrate 7 are flat and smooth enough, the sealing of the liquid in the channel groove 204 can be realized under the action of pressure. Because the chemical reaction only takes place in the runner, and the interior volume of runner is little, so use this device can save reaction reagent.
As shown in FIG. 4, after the surface modification biochemical reaction is completed, the opening of the fixing groove can be placed upwards to form a small pool capable of containing liquid, and the fixing groove is filled with volatile pure common pure solvents such as absolute ethyl alcohol, so that the upper and lower surface pressures of the gene sequencing chip substrate 7 are balanced with the pressure in the solvent, and the gene sequencing chip substrate can be taken out easily. Since the injected solvent is volatile and pure, an operator can blow dry or dry the gene sequencing chip substrate 7 with high-purity nitrogen gas to keep the surface of the gene sequencing chip substrate 7 clean.
This application can completely cut off air pollution, and simple structure, convenient operation, split are convenient simultaneously, make and avoid the test result to receive air contaminant or other uncertain factor influences when laboratory preparation gene sequencing chip to improve the efficiency of experiment.
Principle of operation
The gene sequencing chip substrate 7 is clamped between the upper pressing plate 6 and the lower pressing plate 8 and is fixed in the fixing clamp 5, and a closed flow channel is formed between the flow channel groove 204 of the upper pressing plate 6 and the gene sequencing chip substrate 7 under the action of pressure and is used for the circulation of biochemical reaction reagents. The biochemical reaction reagent enters the liquid inlet 201 from the liquid inlet conduit 1 through the liquid inlet joint 3, and then flows into the flow channel groove 204, and the surface modification chemical reaction of the gene sequencing chip substrate 7 occurs in the flow channel. After the reaction is finished, the reaction liquid flows out from the liquid outlet 203 through the liquid outlet joint 4 and the liquid outlet conduit 2.
In the description of the present application, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience in describing the present application and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present application.
The foregoing description of the specific embodiments of the invention has been presented. It is to be understood that the present invention is not limited to the specific embodiments described above, and that various changes or modifications may be made by one skilled in the art within the scope of the appended claims without departing from the essential spirit of the invention. The embodiments and features of the embodiments of the present application may be combined with each other arbitrarily without conflict.
Claims (10)
1. A substrate surface modification apparatus for a gene sequencing chip, comprising: an upper pressing plate (6), a gene sequencing chip substrate (7), a lower pressing plate (8) and a fixing clamp (5);
the gene sequencing chip substrate (7) is clamped between the upper pressing plate (6) and the lower pressing plate (8);
the gene sequencing chip substrate (7) is hermetically connected with the upper pressing plate (6), and a flow channel is formed between the gene sequencing chip substrate and the upper pressing plate;
the gene sequencing chip substrate (7), the upper pressing plate (6) and the lower pressing plate (8) are all fixed in the fixing clamp (5);
and a channel is arranged in the fixing clamp (5), the channel is communicated with the outside, and the channel is communicated with the flow channel.
2. The apparatus for modifying the surface of a substrate for a gene sequencing chip according to claim 1, wherein a flow channel groove (204) is provided on the lower surface of the upper platen (6).
3. The apparatus for modifying the surface of a substrate for a gene sequencing chip according to claim 2, wherein the upper platen (6) is provided at both ends thereof with a liquid inlet (201) and a liquid outlet (203), respectively, and both the liquid inlet (201) and the liquid outlet (203) are communicated with the channel groove (204).
4. The apparatus for modifying the surface of a substrate for a gene sequencing chip according to claim 3, wherein a liquid inlet joint (3) and a liquid outlet joint (4) are respectively disposed at two ends of the upper surface of the fixing clamp (5), the liquid inlet joint (3) is disposed corresponding to and communicated with the liquid inlet (201), and the liquid outlet joint (4) is disposed corresponding to and communicated with the liquid outlet (203).
5. The apparatus for modifying the surface of a substrate for a gene sequencing chip according to claim 4, wherein the liquid inlet connector (3) is connected to a liquid inlet conduit (1), and the liquid outlet connector (4) is connected to a liquid outlet conduit (2).
6. The substrate surface modification apparatus for a gene sequencing chip according to claim 1, wherein the upper platen (6) is provided with a detachment notch (202) at both sides of the bottom.
7. The apparatus for modifying a substrate surface for a gene sequencing chip of claim 1, wherein a fixing groove recessed inward is provided at one side of the fixing jig (5), and a position-limiting step (301) is provided in the fixing groove.
8. The apparatus for modifying the surface of a substrate for a gene sequencing chip of claim 1, wherein a set screw (9) is provided on the lower surface of the fixing jig (5).
9. The apparatus for modifying the surface of a substrate for a gene sequencing chip according to claim 1, wherein the upper platen (6) comprises a transparent antiseptic rigid plate having a smooth and flat lower surface;
the lower pressing plate (8) comprises a transparent rigid corrosion-resistant plate, and the upper surface of the transparent rigid corrosion-resistant plate is smooth and flat;
the fixing clamp (5) comprises an anti-corrosion rigid clamp.
10. The apparatus for modifying the surface of a substrate for a gene sequencing chip according to claim 1, wherein a base (10) is provided at the bottom of the fixing jig (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221389905.9U CN217910484U (en) | 2022-06-06 | 2022-06-06 | Substrate surface modification device for gene sequencing chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221389905.9U CN217910484U (en) | 2022-06-06 | 2022-06-06 | Substrate surface modification device for gene sequencing chip |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217910484U true CN217910484U (en) | 2022-11-29 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221389905.9U Active CN217910484U (en) | 2022-06-06 | 2022-06-06 | Substrate surface modification device for gene sequencing chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN217910484U (en) |
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2022
- 2022-06-06 CN CN202221389905.9U patent/CN217910484U/en active Active
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