CN102043043A - Multi-project biochemical test analysis integrated kit and method thereof - Google Patents
Multi-project biochemical test analysis integrated kit and method thereof Download PDFInfo
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- CN102043043A CN102043043A CN2010105840708A CN201010584070A CN102043043A CN 102043043 A CN102043043 A CN 102043043A CN 2010105840708 A CN2010105840708 A CN 2010105840708A CN 201010584070 A CN201010584070 A CN 201010584070A CN 102043043 A CN102043043 A CN 102043043A
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Abstract
The invention discloses a multi-project biochemical test analysis integrated kit and a method thereof. The integrated kit comprises a kit body, wherein the kit body is provided with a plurality of containing cups, multiple reagents for detection are previously placed in the containing cups, and the reagents are respectively sealed in the containing cups by one overlay film. By using the multi-project biochemical test analysis integrated kit and the method thereof, operating personnel only need to add the reagents in the containing cups to samples to be detected because the biochemical detection reagents for multiple projects are previously filled in the containing cups of the kit. When being matched with a biochemical analyzer, the invention simplifies the operating processes of the operating personnel and can realize fast biochemical analysis and detection without any requirement for professional knowledge levels of the operating personnel.
Description
Technical field
The present invention relates to a kind of external biochemistry detection in analyzing kit device commonly used with and analyzing detecting method, in particular a kind of integrated kit and analytical approach when needing multiple biochemical indicator to analyze simultaneously at certain disease.
Background technology
The common every biochemical project inspection of hospital laboratory has become the indispensable diagnostic means of clinician.These common projects mainly comprise following a few class according to the clinical diagnosis meaning.
The project that for example reflects patient's liver function, glutamic-pyruvic transaminase (ALT), glutamic-oxalacetic transaminease (AST), alkaline phosphatase (ACP), the gamma glutamyl transpeptidase (γ-GT) that comprises serum, total bilirubin (T-BIL), bilirubin direct (D-BIL), albumin (ALB), total protein (TP) etc.According to statistics, the hepatitis B incidence of disease in the whole nation in 2007 is 89.00/10 ten thousand, does not also comprise the hepatitis carrier of One's name is legion.Hepatitis B patient need regularly carry out the liver function item inspection, is converted into cirrhosis and liver cancer with prevention hepatitis.
For example the diabetes project comprises serum blood sugar (GLU), carbohydrate tolerance test (OGTT) again, fructosamine (FA), glycosylated hemoglobin (HbA1c) etc.Average attack rate according to data China l type diabetes of IDF (IDF) issue be (0.59/10 ten thousand), and the incidence of disease is the trend of increase year by year.Diabetic needs the concentration of glucose in the periodic monitoring serum to avoid blood sugar level too high or too low.
For example the lipid examination project comprises serum triglyceride (TG), T-CHOL (TCHO), high density cholesterol (HDL-C), low density cholesterol (LDL-C) etc. again.Along with China's resident living level improves, obese people quantity is also increasing sharply, the blood fat that obesity causes raises not only can cause the generation of angiocardiopathy, and can bring out type II diabetes, and now a lot of people are with the essential project of lipids detection as health examination.
The project that also for example reflects patient's renal function comprises serum urea nitrogen (BUN), creatinine (CR), uric acid (UA) etc.Patients such as ephritis, renal diabetes, renal hypertension need regularly to carry out renal function and detect.
As seen, these biochemical investigation projects not only have most important effect in clinical diagnosis, also with the prevention and the control of a lot of diseases inseparable relation are arranged.Measure these projects at present and all must use some special-purpose analytical instrument, as automatic clinical chemistry analyzer, semi-automatic biochemical analyzer.
In the big-and-middle-sized hospital of China, its better economic benefit, generally all use automaticity height, test speed fast, measure flux height, expensive, the bulky automatic clinical chemistry analyzer of price.
And for some small medical mechanisms such as She Kang center, city, villages and towns in rural areas commune hospital etc., owing to funds and place reason, use the semi-automatic biochemical analyzer that price is low, volume is less mostly, but it is loaded down with trivial details relatively, high to operating personnel's operative skill and specialty requirement that automanual analyser is operated, and the instrument user of service of these small medical mechanisms does not generally pass through good professional training, degree of accuracy is very poor as a result to use automanual instrument to measure it, often can't obtain satisfied result.
Present biochemical reagents are generally held in the big Packaging Bottle that is used for many person-portions uses, operating personnel are during at multinomial range estimation amount, need to use several different reagent, to obtain different measurement data, like this, just need earlier a certain detected sample to be put into a plurality of reaction cup successively, use filling apparatus from the Packaging Bottle of correspondence, to draw respectively required different reagent then, add in the reaction cup respectively one by one again, this operating process is very complicated, and requires operating personnel to have higher professional standards.
In addition, the packaged reagent of many people also has weak point: reagent all has certain use term of validity after opening bag, if reagent does not exhaust before the deadline, remaining reagent will lose efficacy, and caused reagent waste, and then increases inspection cost.
With the liver function six (glutamic-pyruvic transaminase, glutamic-oxalacetic transaminease, total bilirubin, bilirubin direct, albumin, total proteins) of measuring a sample is example, and the loaded down with trivial details of its process is described.The checked operation personnel at first need to prepare 6 test tubes, add a certain amount of blood sample earlier in each test tube, add reagent corresponding separately then respectively in each test tube; Adding finishes, and puts into incubator again and makes its reaction regular hour; And then, take in the semi-automatic biochemical analyzer respectively and detect the invisible spectro reaction product of difference.This process is very complicated, and consuming time.
Because semi-automatic biochemical analyzer complex operation, require very high to operating personnel's operative skill, and automatic clinical chemistry analyzer costs an arm and a leg, volume is big, in the system of the She Kang center in city, all be every day sample to be put together to deliver to the large hospital that it belongs to generally at present, centralab by large hospital carries out Measurement and analysis with full automatic instrument, the result is delivered to the She Kang center then in second day again.But the drawback of this working method is the doctor at She Kang center can't in time obtain patient's testing result, and has prolonged the time that patient goes to a doctor.In order to obtain accurately and testing result timely, a lot of patients do not stint to seek something far and wide when it is within reach and go to large hospital to go to a doctor, and this has just caused overstaffed and She Kang center and the health clinics in towns and townships phenomenon that you can catch sparrows on the doorstep of each big general hospital of China.This prescription on individual diagnosis pattern had both been wasted the resource of large hospital, had also increased patient's unnecessary economy spending, caused the unbalanced of medical resource use.
Therefore, develop a kind of convenience and simplify the operation, operative skill is required low kit and detection method, can carry out common biochemical project inspections such as liver function, renal function, blood sugar, blood fat in places such as She Kang center, city and villages and towns in rural areas commune hospitals, have very important significance.
Summary of the invention
The object of the present invention is to provide a kind of multinomial order biochemical investigation to analyze integrated kit and method thereof, a kind of multinomial range estimation quantity set is set becomes kit, some the project reagent that will measure are packed in advance according to certain amount in the integrated kit, the user only needs to add quantified sample in use, then this kit is put into the biochemical analysis surveying instrument, automatically according to the time of reagent requirement reagent is carried out incubation and measurement by this surveying instrument, so just can remove operating personnel from need be to not being both the trouble of adding reagent in the reaction cup.
Technical scheme of the present invention is as follows:
Integrated kit is analyzed in a kind of multinomial order biochemical investigation, it comprises a box body, and wherein, described box body is provided with several and holds cup, be placed with the plurality of reagents required in the cup in advance in described holding, and described reagent be enclosed in described holding in the cup respectively by a coverlay according to detection.
Described integrated kit, wherein, described a plurality of cups that hold are according to the line spread setting.
Described integrated kit, wherein, described a plurality of cups that hold are arranged setting according to camber line.
Described integrated kit, wherein, check is provided with described kit at liver function, and described reagent comprises the reagent of glutamic-pyruvic transaminase ALT, the glutamic-oxalacetic transaminease AST, alkaline phosphatase ACP, gamma glutamyl transpeptidase γ-GT, total bilirubin T-BIL, bilirubin direct D-BIL, albumin A LB and the total protein TP that measure serum.
Described integrated kit, wherein, check is provided with described kit at diabetes, and described reagent comprises the reagent of measuring serum blood sugar GLU, carbohydrate tolerance test OGTT, fructosamine FA and glycosylated hemoglobin HbA1c.
Described integrated kit, wherein, described kit is provided with at the lipid examination project, and described reagent comprises the reagent of measuring serum triglyceride TG, T-CHOL TCHO, high density cholesterol HDL-C and low density cholesterol LDL-C.
Described integrated kit, wherein, check is provided with described kit at the renal function project, and described reagent comprises the reagent of measuring serum urea nitrogen BUN, creatinine CR and uric acid UA.
The method that a kind of multinomial order biochemical investigation is analyzed, it may further comprise the steps:
A, several are set hold cup, and one is arranged and is provided with;
B, place in advance in the cup according to detecting required plurality of reagents, and described reagent is enclosed in described holding in the cup respectively by coverlay in described holding;
C, the detected material sample added to hold in the cup according to aequum react;
D, use biochemical analyzer carry out biochemical analysis to the reaction afterproduct.
Useful technique effect of the present invention is as follows: integrated kit and method thereof are analyzed in a kind of multinomial order biochemical investigation provided by the present invention, because employing is encapsulated in a plurality of of kit in advance with multinomial purpose biochemistry detection reagent and holds in the cup, only the reagent that holds in the cup is added testing sample, cooperate biochemical analyzer, simplified operating personnel's operating process, need not the operator have professional level, can realize quick biochemical analysis, patient can carry out some routines, the regular project that needs inspection nearby on the one hand; On the other hand, the doctor can in time obtain patient's testing result, formulates the proper treatment scheme, alleviates the phenomenon of China's difficulty and high cost of getting medical, the high cost of getting medical treatment to a certain extent.
In addition, because integrated kit of the present invention is single packaged use, overcome the reagent waste that many person-portions kit can not exhaust before the deadline and cause in the prior art after opening bag, both made things convenient for the preservation of reagent to use, reduce the waste of reagent again, further reduced inspection cost.
Description of drawings
Fig. 1 analyzes the synoptic diagram of integrated kit preferred embodiment for the multinomial order biochemical investigation of the present invention.
Fig. 2 is the A-A cross section view of integrated kit shown in Fig. 1.
Fig. 3 analyzes the synoptic diagram of integrated another preferred embodiment of kit for the multinomial order biochemical investigation of the present invention.
Fig. 4 is the schematic flow sheet of the method for the multinomial order biochemical investigation analysis of the present invention.
Embodiment
Below preferred embodiment of the present invention is described in detail.
In the integrated kit of the present invention, be placed with in advance in the cup 120 according to detecting required plurality of reagents 130 in described holding, each holds cup and places needed a kind of reagent respectively, and by a coverlay 121 described reagent 130 is enclosed in described holding in the cup 120, as shown in Figure 2 respectively.
The present invention is used for the integrated kit of biochemical analysis, and its production run can robotization, according to the production of finalizing the design respectively of the project of required detection; Transportation after the production is also very convenient, according to preservation condition different packing cases can be set, and for example meets temperature and illuminance requirement etc.Daily can the storage in the corresponding packing case when using, taken out a corresponding integrated kit, earlier coverlay torn, and detected sample joined successively hold in the cup, and hold cup this moment also is reaction cup simultaneously.So also reduce the waste that many person-portions package kit lost efficacy and causes because of reagent, and then reduced the detection cost.
In the preferred embodiment, can also be provided with an identified areas on the integrated kit of the present invention, be used for showing some information of this kit, for example be used for which kind of detection, storage conditions or the like, identified areas can be arranged on the lateral wall of described integrated kit, also can be arranged on the coverlay of described integrated kit.
When check is provided with at liver function, need to be provided with described reagent in the integrated kit of the present invention, can include but not limited to measure the reagent of glutamic-pyruvic transaminase ALT, glutamic-oxalacetic transaminease AST, alkaline phosphatase ACP, gamma glutamyl transpeptidase γ-GT, total bilirubin T-BIL, bilirubin direct D-BIL, albumin A LB and the total protein TP of serum.
When check was provided with the integrated kit of the present invention at diabetes, described reagent can include but not limited to measure the reagent of serum blood sugar GLU, carbohydrate tolerance test OGTT, fructosamine FA and glycosylated hemoglobin HbA1c.
When at the lipid examination project kit of the present invention being set, described reagent can include but not limited to measure the reagent of serum triglyceride TG, T-CHOL TCHO, high density cholesterol HDL-C and low density cholesterol LDL-C.
When check was provided with kit of the present invention at the renal function project, described reagent can include but not limited to measure the reagent of serum urea nitrogen BUN, creatinine CR and uric acid UA.
The method that the present invention also provides a kind of multinomial order biochemical investigation to analyze simultaneously, as described in Figure 4, it may further comprise the steps:
Step 101: several is set holds cup, and one is arranged in row or column, can straight line or camber line arrange;
Step 102: place the plurality of reagents required in the cup in advance in described holding, and described reagent is enclosed in described holding in the cup respectively by coverlay according to detection;
Step 103: the detected material sample added to hold in the cup according to aequum react;
Step 104: use biochemical analyzer that the reaction afterproduct is carried out biochemical analysis.
The analytic process of described biochemical analyzer is known by prior art, does not therefore repeat them here.
Integrated kit of the present invention and method have made things convenient for manipulating of operator, and operator's professional requirement is very low, and running program is simply easy, and have reduced the possibility of maloperation.
Should be understood that, for those of ordinary skills, can be improved according to the above description or conversion, and all these improvement and conversion all should belong to the protection domain of claims of the present invention.
Claims (8)
1. integrated kit is analyzed in a multinomial order biochemical investigation, it comprises a box body, it is characterized in that, described box body is provided with several and holds cup, be placed with the plurality of reagents required in the cup in advance in described holding, and described reagent be enclosed in described holding in the cup respectively by a coverlay according to detection.
2. integrated kit according to claim 1 is characterized in that, described a plurality of cups that hold are arranged setting according to camber line.
3. integrated kit according to claim 1 is characterized in that, described a plurality of cups that hold are according to the line spread setting.
4. integrated kit according to claim 1 is characterized in that, check is provided with described kit at liver function, and described reagent comprises glutamic-pyruvic transaminase ALT, the glutamic-oxalacetic transaminease AST that measures serum, the reagent of gamma glutamyl transpeptidase γ-GT.
5. integrated kit according to claim 1 is characterized in that, check is provided with described kit at diabetes, and described reagent comprises the reagent of measuring serum blood sugar GLU and glycosylated hemoglobin HbA1c.
6. integrated kit according to claim 1, it is characterized in that, described kit is provided with at the lipid examination project, and described reagent comprises the reagent of measuring serum triglyceride TG, T-CHOL TCHO, high density cholesterol HDL-C and low density cholesterol LDL-C.
7. integrated kit according to claim 1 is characterized in that, check is provided with described kit at the renal function project, and described reagent comprises the reagent of measuring serum urea nitrogen BUN, creatinine CR and uric acid UA.
8. method that multinomial order biochemical investigation is analyzed, it may further comprise the steps:
A., several are set hold cup, and one is arranged setting;
B. place the plurality of reagents required in the cup in advance in described holding, and described reagent is enclosed in described holding in the cup respectively by coverlay according to detection;
C. the detected material sample is added to hold in the cup according to aequum and react;
D. use biochemical analyzer that the reaction afterproduct is carried out biochemical analysis.
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| CN2010105840708A CN102043043A (en) | 2010-12-10 | 2010-12-10 | Multi-project biochemical test analysis integrated kit and method thereof |
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| CN2010105840708A CN102043043A (en) | 2010-12-10 | 2010-12-10 | Multi-project biochemical test analysis integrated kit and method thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105807040A (en) * | 2016-05-31 | 2016-07-27 | 四川金域医学检验中心有限公司 | Reagent device for biochemical index detection |
| CN106018768A (en) * | 2016-05-31 | 2016-10-12 | 四川金域医学检验中心有限公司 | Multi-project biochemical examination and analysis integrated kit |
| CN106053866A (en) * | 2016-05-31 | 2016-10-26 | 四川金域医学检验中心有限公司 | Multi-item biochemical detection analysis method |
| CN106066396A (en) * | 2016-05-31 | 2016-11-02 | 四川金域医学检验中心有限公司 | A kind of kit being easy to Analysis of Biochemical |
| CN113164110A (en) * | 2019-09-17 | 2021-07-23 | 诺尔生物医药有限公司 | System and method for measuring liver enzyme levels in blood |
| CN114295850A (en) * | 2021-12-31 | 2022-04-08 | 沈阳医陆生物科技有限公司 | Sample detection system, sample detection method and kit preparation method |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105807040A (en) * | 2016-05-31 | 2016-07-27 | 四川金域医学检验中心有限公司 | Reagent device for biochemical index detection |
| CN106018768A (en) * | 2016-05-31 | 2016-10-12 | 四川金域医学检验中心有限公司 | Multi-project biochemical examination and analysis integrated kit |
| CN106053866A (en) * | 2016-05-31 | 2016-10-26 | 四川金域医学检验中心有限公司 | Multi-item biochemical detection analysis method |
| CN106066396A (en) * | 2016-05-31 | 2016-11-02 | 四川金域医学检验中心有限公司 | A kind of kit being easy to Analysis of Biochemical |
| CN113164110A (en) * | 2019-09-17 | 2021-07-23 | 诺尔生物医药有限公司 | System and method for measuring liver enzyme levels in blood |
| CN114295850A (en) * | 2021-12-31 | 2022-04-08 | 沈阳医陆生物科技有限公司 | Sample detection system, sample detection method and kit preparation method |
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Application publication date: 20110504 |