CN1390953A - Biochip compatible with 96 pore plate operating system - Google Patents
Biochip compatible with 96 pore plate operating system Download PDFInfo
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- CN1390953A CN1390953A CN 02112228 CN02112228A CN1390953A CN 1390953 A CN1390953 A CN 1390953A CN 02112228 CN02112228 CN 02112228 CN 02112228 A CN02112228 A CN 02112228A CN 1390953 A CN1390953 A CN 1390953A
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- solid substrate
- biochip
- operating system
- holes
- plate operating
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Abstract
A biochip compatible with the automatic operating system of 96-hole enzyme-linked board is composed of solid substrate and perforated frame structure. Said solid substrate is a glass plate, which has been aldehydized or ammonified and treated by biotin and chain avidin for binding biologic moleculae or biologic tissue blocks. Said perforated frame has the sizes same as those of 96-hole enzyme-linked board.
Description
Technical field
The biochip of the present invention and 96 hole enzyme label plate operating system compatibilities relates to a kind of biochip that can carry out processing such as application of sample, reaction, cleaning in 96 orifice plate operating systems.Biochip be a kind of on solid substrate the biological sample of ordered arrangement, to realize biomolecules or to organize the device of parallel detection, identification, evaluation and diagnosis.Can be applicable to biology, medical science and association area.
Background technology
The research of biochip, exploitation have become the hot subject of domestic and international biology and medical research, how can realize that Highgrade integration, parallelization, automatization, little biological respinse of finishing quantitatively are the problems that especially causes concern in this problem.
The many biochips of development and application mainly are directly to carry out point sample, processing on solid-state upholder at present, and a chip once can only react with a non-solid sample; Though multiple-sample microarray biochip once can with several even dozens of example reaction, but on the previous chip of order the reality parallel work-flow at most only be more than 30.What is more important though above-mentioned biochip has improved efficient greatly than single operation, to application of sample, reaction, cleaning, still needs a large amount of repetitive operations from the preparation of sample, elapsed time not only, the error that also has human factor to cause.Though the Affymetrics company of the U.S. has developed the reaction workstation, only limit to the gene chip of our company exploitation, one of this chip only can be surveyed a sample, and the preparation of sample needs manual operations fully equally, and the costing dearly of chip and operating system.
At present a lot of laboratories, especially all there is the operating system of 96 orifice plates the inspection center of hospital, possesses the advantage of automatization, parallelization, if integrated, little quantized advantage that can have in conjunction with biochip itself is just can advance the efficient and the accuracy of experiment greatly.
Summary of the invention
The object of the present invention is to provide a kind of biochip that can in 96 hole enzyme label plate operating system, operate,, parallelization integrated, automatization, little biological respinse of finishing quantitatively with further realization.
Purpose of the present invention can realize by following technical proposal: a kind of biochip of and 96 orifice plate operating system compatibilities, form by solid substrate and skeleton construction with holes, described solid substrate is to handle through aldehyde radicalization or amination, vitamin H, streptavidin isoreactivity group, but the sheet glass of binding biomolecules or biological tissue's piece; Described skeleton construction with holes is consistent with the distance at length, pitch of holes and the width between centers edge, hole of 96 orifice plates.
Described solid substrate is 11 to 12.5 centimetres of 4 7.5cm * 2.5cm slide glass arranged side by side or lengths of a film, wide 7.5 to 8 centimetres sheet glass.
On the basis of technique scheme, framework with holes of the present invention can be made of polystyrene, and profile is consistent with 96 orifice plates, and the hole can be for circular or square, 0.3 to 1.5 centimetre of hole depth, the no end.Base of frame with holes and glass tight joint are closely glued together as cementing agent and glass, and described being glued at when experimental implementation finishes can remove maybe and can not remove.
On the basis of technique scheme, framework with holes of the present invention can also be by supporting part and grill portion to form, the described part of supporting is that the length that is made of polystyrene is consistent with 96 orifice plates, the structure of hollow, apart from the top 0.3 to 1.5 centimeters have can chimeric described solid substrate support, described grill portion is made of materials such as polystyrene or rubber, size is consistent with solid substrate, band circular port or square opening, one-side band glue, glue-bondable in solid substrate, gummed back pitch of holes and hole width between centers support that the distance of part edge is consistent with 96 orifice plates.
The biochip of the present invention and 96 orifice plate operating system compatibilities is through behind the point sample, can directly place 96 orifice plates application of sample, react, wash automation operating systems such as plate and operate, place special scanning system to read the result whole 96 orifice plate chips behind the EO and analyze, perhaps solid substrate is separated and read the result in the chip scanner that is placed on specialty and analyze.
The present invention is as follows with the superiority of the biochip of 96 hole enzyme label plate operating system compatibilities: the reaction pattern of biochip and the automation operating system of 96 hole enzyme plates are combined, can carry out the parallel detection of many indexs, multiple sample simultaneously.The index number depends on counting of each aperture, and sample number can reach 96.What is more important owing to can be used for the operating system of automatization, has been liberated manpower, has improved the accuracy and the stability of reaction; And, because this biochip reaction pattern and existing clinical instrumentation coupling need not be acquired expensive machines equipment, offer convenience for the marketing after the commercialization.
Description of drawings
Accompanying drawing 1 is with the biochip vertical view of 96 hole enzyme label plate operating system compatibilities.
Accompanying drawing 2 is with the side sectional view of the biochip of 96 hole enzyme label plate operating system compatibilities.
Wherein: 1, framework with holes; 2, circular aperture; 3, solid substrate, i.e. sheet glass.
Embodiment
Embodiment one:
As accompanying drawing 1 and accompanying drawing 2, the biochip of the present invention and 96 hole enzyme label plate operating system compatibilities is made up of solid substrate 3 and skeleton construction with holes 1, described solid substrate is to handle through aldehyde radicalization or amination, vitamin H, streptavidin isoreactivity group, but the sheet glass of binding biomolecules or biological tissue's piece; Described skeleton construction with holes is consistent with the distance at length, pitch of holes and the width between centers edge, hole of 96 orifice plates.Solid substrate and framework with holes be common to form the aperture 2 of separating mutually but can seepage.
In the present embodiment, solid substrate is the sheet glass of 0.096 centimetre of 12 cm x, 8 cm x.Framework with holes is made of polystyrene, and profile is consistent with 96 orifice plates, 1 centimetre of hole depth, and the no end,, the aperture number is 12 * 8 totally 96.Base of frame with holes is closely glued together by cementing agent and glass, and described being glued at when experimental implementation finishes can remove maybe and can not remove.
Chip is through behind the point sample, can directly place 96 orifice plates application of sample, react, wash automation operating system such as plate and operate, behind the EO, place special scanning system to read the result entire chip and analyze.
Embodiment two:
Element such as embodiment one, Frame Design with holes is a square opening, 0.9 centimetre of the aperture length of side.
Embodiment three:
Solid substrate is made of 4 standard slide glasss, 0.096 centimetre of every 7.5 cm x 2.5 cm x.
Framework with holes is by supporting part and grill portion to form, the described part of supporting is that the length that is made of polystyrene is consistent with 96 orifice plates, the structure of hollow, apart from the top 0.3 to 1.5 centimeters have can chimeric described solid substrate support, described grill portion is made of polystyrene or rubber, size is consistent with solid substrate, band circular port or square opening, one-side band glue, glue-bondable in solid substrate, gummed back pitch of holes and hole width between centers support that the distance of part edge is consistent with 96 orifice plates.
The making and use method of present embodiment is as follows: at first with grill portion and solid substrate gummed, place the support corresponding site of framework support part with holes, behind the point sample, can directly place 96 orifice plates application of sample, react, wash automation operating systems such as plate and operate, behind the EO, take out the grill portion and the solid substrate of gummed, solid substrate is separated read the result in the chip scanner that is placed on specialty and analyze.
Claims (7)
1, a kind of biochip of and 96 hole enzyme label plate operating system compatibilities, form by solid substrate and skeleton construction with holes, it is characterized in that, described solid substrate is to handle through a kind of active group in aldehyde radicalization or amination, vitamin H, the streptavidin, but the sheet glass of binding biomolecules or biological tissue's piece; Described skeleton construction with holes is consistent with the distance at length, pitch of holes and the width between centers edge, hole of 96 orifice plates.
2, the described biochip with 96 hole enzyme label plate operating system compatibilities of claim 1 is characterized in that, described solid substrate is 11 to 12.5 centimetres of 4 7.5cm * 2.5cm slide glass arranged side by side or lengths of a film, wide 7.5 to 8 centimetres sheet glass.
3, the described biochip with 96 orifice plate operating system compatibilities of claim 1 is characterized in that described framework with holes is made of polystyrene, and profile is consistent with 96 orifice plates, and the hole can be a circle or square, 0.3 to 1.5 centimetre of hole depth, the no end.
4, the described biochip with 96 orifice plate operating system compatibilities of claim 3 is characterized in that described base of frame with holes and glass tight joint.
5, the described biochip with 96 orifice plate operating system compatibilities of claim 1, it is characterized in that, described framework with holes is by supporting part and grill portion to form, described support part is the length that is made of polystyrene, wide, high consistent with 96 orifice plates, the structure of hollow, apart from the top 0.5 to 1.5 centimeters have can chimeric described solid substrate support, described grill portion is made of materials such as polystyrene or rubber, size is consistent with solid substrate, band circular port or square opening, one-side band glue, glue-bondable in solid substrate, gummed back pitch of holes and hole width between centers support that the distance of part edge is consistent with 96 orifice plates.
6, the making method of the described biochip with 96 orifice plate operating system compatibilities of claim 5 is; At first, place the support corresponding site of framework support part with holes, carry out point sample grill portion and solid substrate gummed.
7, the using method of the described biochip with 96 orifice plate operating system compatibilities of claim 1, it is characterized in that, behind the point sample of described biochip solid substrate surface, can directly place 96 orifice plates application of sample, react, wash the affected systems of automatization such as plate and operate, behind the EO, place special scanning system to read the result whole 96 orifice plate chips and analyze, perhaps solid substrate is separated and read the result in the chip scanner that is placed on specialty and analyze.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02112228 CN1390953A (en) | 2002-06-25 | 2002-06-25 | Biochip compatible with 96 pore plate operating system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02112228 CN1390953A (en) | 2002-06-25 | 2002-06-25 | Biochip compatible with 96 pore plate operating system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1390953A true CN1390953A (en) | 2003-01-15 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 02112228 Pending CN1390953A (en) | 2002-06-25 | 2002-06-25 | Biochip compatible with 96 pore plate operating system |
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| CN (1) | CN1390953A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102043043A (en) * | 2010-12-10 | 2011-05-04 | 张会生 | Multi-project biochemical test analysis integrated kit and method thereof |
-
2002
- 2002-06-25 CN CN 02112228 patent/CN1390953A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102043043A (en) * | 2010-12-10 | 2011-05-04 | 张会生 | Multi-project biochemical test analysis integrated kit and method thereof |
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