CN108693370B

CN108693370B - Sample injection module for microfluidic chip and NC membrane multi-flux detection

Info

Publication number: CN108693370B
Application number: CN201810410726.0A
Authority: CN
Inventors: 许行尚; 杰弗瑞·陈; 应家树; 张前军
Original assignee: Nanjing Lanyu Biological Technology Co Ltd
Current assignee: Nanjing Lanyu Biological Technology Co Ltd
Priority date: 2018-05-02
Filing date: 2018-05-02
Publication date: 2023-06-16
Anticipated expiration: 2038-05-02
Also published as: CN108693370A

Abstract

The invention discloses a sample injection module, which is internally provided with a chip sample injection clamping groove and an NC film sample injection clamping groove which are mutually independent and are respectively used for inserting a chip or an NC film clamping strip; the sample injection module is also provided with a liquid path and gas path module; a chip sample injection shifting hand is arranged on the side surface of the chip sample injection clamping groove and fixedly connected to a first transmission connecting block, and the first transmission connecting block is driven by a chip sample injection shifting hand force motor to move back and forth through a synchronous belt; the NC film sample introduction clamping groove is provided with an NC film sample introduction hand on the side face, the NC film sample introduction hand is fixedly connected to a second transmission connecting block, and the second transmission connecting block is driven by the NC film sample introduction hand power motor to move back and forth through a synchronous belt. The liquid path gas path module in the sample injection module is used for ensuring that the chip reaction bin is full of samples and reagents for reaction; the technology application scene of the micro-fluidic chip and NC film detection is considered, and the simultaneous sample injection of two measuring platforms of the micro-fluidic chip and the NC film detection is satisfied.

Description

Sample injection module for microfluidic chip and NC membrane multi-flux detection

Technical Field

The invention belongs to the technical field of medical equipment, and particularly relates to a sample injection module for multi-flux detection of a microfluidic chip and an NC (numerical control) membrane.

Background

Microfluidic chip (microfluidic chip) is a hotspot field for the development of current micro total analysis systems (Miniaturized Total Analysis Systems). The microfluidic chip analysis uses a chip as an operation platform, simultaneously uses analytical chemistry as a basis, relies on a micro-electromechanical processing technology, uses a micro-pipe network as a structural feature, uses life science as a main application object at present, and is an important point of development in the field of the current micro-total analysis system. Its goal is to integrate the functions of the whole laboratory, including sampling, dilution, reagent addition, reaction, separation, detection, etc., on a microchip. The micro-fluidic chip is a main platform for micro-fluidic technology realization. The device is characterized by its effective structure (channels, reaction chambers and other certain functional components) for containing fluids, which is at least on a micrometer scale in one dimension. Due to the micro-scale structure, the fluid exhibits and produces therein specific properties that differ from those of the macro-scale. Thus developing unique analytical properties. Characteristics and development advantages of the microfluidic chip: the microfluidic chip has the characteristics of controllable liquid flow, extremely small consumption of samples and reagents, ten times or hundreds times higher analysis speed and the like, can simultaneously analyze hundreds of samples in a few minutes or even shorter, and can realize the whole pretreatment and analysis processes of the samples on line. The generated application purpose is to realize the final target of the micro total analysis system, namely a chip laboratory, and the key application field of the current work and development is the life science field.

Current international research status: innovation is focused on the aspects of separation and detection systems; the problems of how to introduce the analysis of the actual sample on the chip, such as the sample introduction, sample changing, pretreatment and other related researches, are very weak. Its development depends on the development of multidisciplinary crossover.

NC membrane is a detection technology commonly used in biomedical inspection at present, and the technology is to perform qualitative and quantitative detection by utilizing the characteristic that an analyte generates fluorescence under the excitation of light with a specific wavelength. Because the fluorescence immunoassay technology has a series of advantages of high sensitivity, strong specificity, high detection speed, safety, stability and the like, the fluorescence immunoassay technology is widely applied to clinical detection, and has wide application prospects in the detection fields of endocrinopathy detection, infectious disease detection, gynecological disease detection, tumor marker detection, genetic disease detection, blood and cytology detection and the like.

In chinese patent document CN107843583a, a microfluidic chemiluminescent analyzer and a method of use are disclosed, comprising a liquid path gas path module, a chip loading module, and an ultrasonic module, which are sequentially arranged. The invention integrates a plurality of devices, can sweep the code of each chip and determine personalized detection items; the ultrasonic module is used for fully and uniformly mixing the sample, the reaction reagent and the luminous substrate, and finally, optical detection is carried out, data are read, so that the whole detection program is standardized and automated, the detection sensitivity is high, and the result is reliable. On the basis of ensuring various detection functions, the invention has the advantages of greatly simplified structure, smaller volume, low manufacturing cost and simple and convenient operation, is suitable for being immediately tested at the side of a patient, and can quickly obtain the detection result.

However, the technical scheme does not have the technical application scene of being capable of considering both the micro-fluidic chip and the NC film detection, the micro-fluidic chip detection has the characteristics of high precision, high speed and higher detection cost, is suitable for the detection of accurate medical links, and the NC film detection has the characteristics of high speed, low precision and low detection cost and is generally used for screening detection. Therefore, a new sample injection module for multi-flux detection of a microfluidic chip and an NC membrane needs to be researched.

Disclosure of Invention

The invention aims to solve the technical problem of providing a sample injection module for multi-flux detection of a microfluidic chip and an NC membrane, which is used for simultaneously injecting samples of the microfluidic chip and the NC membrane by taking the technical application scene of the microfluidic chip and the NC membrane detection into consideration.

In order to solve the technical problems, the technical scheme adopted by the invention is that a chip sample injection clamping groove and an NC film sample injection clamping groove which are mutually independent are arranged in the sample injection module and are respectively used for inserting a chip or an NC film clamping strip; the sample injection module is also provided with a liquid path and gas path module;

a chip sample injection shifting hand is arranged on the side surface of the chip sample injection clamping groove and fixedly connected to a first transmission connecting block, and the first transmission connecting block is driven by a chip sample injection shifting hand force motor to move back and forth through a synchronous belt; the NC film sample introduction clamping groove is provided with an NC film sample introduction hand on the side face, the NC film sample introduction hand is fixedly connected to a second transmission connecting block, and the second transmission connecting block is driven by the NC film sample introduction hand power motor to move back and forth through a synchronous belt.

The liquid path gas path module in the sample injection module is used for ensuring that the chip reaction bin is full of samples and reagents for reaction; the chip is inserted into a chip sample introduction clamping groove, the chip sample introduction clamping groove enters the device of the integrated machine and reaches the position, namely, the chip is moved into a liquid path gas path module in a sample introduction module, and the liquid path gas path module processes the chip added with the sample; the chip sample injection shifting hand is arranged on the side face of the chip sample injection clamping groove, so that the chip sample injection shifting hand is driven by the chip sample injection shifting hand to shift the chip into the chip incubation groove for constant-temperature incubation; inserting NC film clamping strips into NC film sample introduction clamping grooves, enabling the NC film sample introduction clamping grooves to enter the device of the all-in-one machine to reach the position, and enabling an NC film sample introduction pulling manual motor to drive an NC film sample introduction pulling hand to pull the NC film clamping strips into an NC film incubation groove for constant-temperature incubation.

Preferably, a first transmission sliding bar is arranged below the first transmission connecting block; a second transmission sliding bar is arranged below the second transmission connecting block; the chip sample injection clamping groove is controlled by a chip sample injection clamping groove control motor, the NC film sample injection clamping groove is controlled by an NC film sample injection clamping groove control motor, and the liquid path gas path module is controlled by a liquid path gas path module control motor to act.

The transmission connecting block moves back and forth along the transmission sliding bar, so that the position is accurate, the movement is stable, and shaking cannot occur; the chip sample feeding clamping groove is adopted to control the motor, the NC film sample feeding clamping groove is adopted to control the motor, the independence between the chip sample feeding clamping groove and the NC film sample feeding clamping groove is kept, and the chip and NC film clamping strips are more convenient and faster to enter; the liquid path gas path module is controlled by the motor controlled by the liquid path gas path module to press down, so that the chip is processed.

Preferably, the liquid path gas path module comprises an upper sliding block, a lower sliding block, a sample injection gas path column, a liquid level rod and a valve pressing rod; the end part of the sample injection gas path column is provided with a gas path sealing piece, and the gas path sealing piece is provided with a sealing ring; the valve pressing rod is controlled by a valve control motor, and the valve pressing rod control motor and the liquid path gas path module control motor are both arranged above the liquid path gas path module.

The upper sliding block and the lower sliding block are arranged to ensure that the direction and the position of the liquid path gas path module for pressing down the chip are accurate, the sample injection gas path column is used for blowing up and pushing samples added into the chip to reach a reaction area under positive pressure, the gas path sealing piece is used for ensuring the sealing reliability of the matched part of the device (the liquid path gas path module) and the chip, and the integrated sealing mode is adopted to maximally meet the sample injection and cleaning sealing requirements of the microfluidic chip; the liquid path column is used for sealing a cleaning liquid port on the chip and preventing the cleaning liquid port from ventilation; the liquid level rod is used for sensing whether the sample reaches and fills the reaction zone; the valve pressing rod is used for immediately making a valve closing response by the valve control motor when the liquid level rod senses that the sample reaches and fills the reaction area, and the valve pressing rod presses down the valve to press down the valve, so that the chip reaction bin is ensured to be full of the sample and the reagent for reaction; the liquid level rod is adopted for liquid level sensing, so that the liquid flow condition inside the microfluidic chip can be accurately sensed, the volumes of samples and cleaning liquid in the microfluidic chip can be mastered in time, and the blocking condition of a pipeline of the microfluidic chip can be automatically judged.

Preferably, a multi-channel rib bridge structure is adopted on the semicircular arc of the sealing ring; the multi-channel rib bridge is arranged, so that the multi-channel rib bridge is easier to be extruded and deformed, and the sealing effect is started.

Preferably, the liquid path gas path module is provided with an upper bracket; the valve pressing rod control motor and the liquid path module control motor are both arranged on the upper bracket; the lower bracket is movably connected with the upper bracket through the upper and lower sliding blocks, wherein the sample injection gas circuit column, the liquid circuit column and the liquid level rod are all arranged on the lower bracket; compact structure, convenient installation and operation.

Preferably, the liquid path gas path module control motor is a screw rod motor, and a screw rod nut matched with a screw rod on the liquid path gas path module control motor is fixedly arranged on the lower support to drive the lower support to slide up and down.

Preferably, the lower bracket is divided into two layers, namely an upper end surface layer and a lower end surface layer, wherein the upper end surface layer is used for installing the screw nut, and a notch is further formed in the upper end surface layer; the valve compression bar is movably inserted in the lower end surface layer, the liquid path column is also connected with a liquid pump, and the sample injection gas path column is also connected with an air pump.

Preferably, the upper bracket is also provided with a lightening hole.

The technical problem to be solved by the invention is to provide a technical application scene which gives consideration to the detection of a microfluidic chip and an NC (numerical control) film, and a device for a microfluidic chip and NC film multi-flux detection integrated machine, which can meet the requirement of simultaneous sample injection of two measurement platforms of the microfluidic chip and the NC film detection.

In order to solve the technical problems, the technical scheme adopted by the invention is that the device for the microfluidic chip and NC membrane multi-flux detection integrated machine comprises a base, wherein a sample injection module, an incubation module, a cleaning module, an NC membrane detection module and a chip detection module are arranged on the base;

the sample injection module is provided with a chip sample injection clamping groove and an NC film sample injection clamping groove which are mutually independent and are respectively used for inserting a chip or an NC film clamping strip; the sample injection module is also provided with a liquid path and gas path module;

the incubation module is provided with a chip incubation groove and an NC membrane incubation groove which are mutually independent, and the sample injection module, the cleaning module and the NC membrane detection module are arranged along the side face of the incubation module in the length direction; and sending the cleaned chips in the cleaning module to the chip detection module for detection.

The liquid path gas path module in the sample injection module is used for ensuring that the chip reaction bin is full of samples and reagents for reaction; chip Wen Yocao, which is used for incubating the chip at constant temperature; similarly, an NC membrane incubation groove is used for incubating NC membrane clamping strips at constant temperature; by adopting the technical scheme, each module component is integrated on the base to form the whole equipment, the structure is integrated, and the technical application scene of the micro-fluidic chip and NC film detection can be considered, wherein the micro-fluidic chip detection has the characteristics of high precision, high speed and higher detection cost, is suitable for the detection of a precise medical link, and the NC film detection has the characteristics of high speed, low precision and low detection cost and is generally used for screening detection; the method can avoid the problem of excessive consumption of medical resources caused by detecting any item by using a high-precision methodology, has the advantages of two technologies, can reasonably detect by combining the actual situation of an application scene, achieves the organic unification of social benefit and economic benefit, can solve the item with low sensitivity requirement by using an NC film lateral chromatography method, can reduce cost and can improve flux; however, the sensitivity requirement and the stability requirement are very high, and the high-difficulty item can be detected by a microfluidic method, so that the sensitivity can be improved, and the accuracy can be achieved. In addition, in the technical scheme of the invention, the microfluidic chip and the NC film are mutually independent and do not interfere, so that simultaneous sample injection of two measurement platforms can be met, the matching requirement of two methods in the system detection process of the POCT detection process can be met, and the detection efficiency can be improved.

Preferably, in the incubation module, the chip incubation groove and NC membrane incubation groove are arranged one above the other; the chip incubation slot has at least one slot for placement of a chip, the chip incubation slot being controlled by a chip incubation slot control motor such that the chip Wen Yocao slides freely along a chip Wen Yocao polish rod; the NC membrane incubation groove has at least one groove position for placing an NC membrane card stripe, and is controlled by an NC membrane incubation groove control motor such that the NC membrane Wen Yocao slides freely along an NC membrane incubation groove polish rod.

The chip incubation groove and the NC film incubation groove are arranged in an up-down overlapping way, so that the structure is compact, the independence of the chip incubation groove and the NC film incubation groove is reflected, and the chip incubation groove and the NC film incubation groove are not interfered; the chip Wen Yocao freely slides along the polish rod of the chip Wen Yocao, and the sample injection module, the cleaning module and the chip detection module are arranged along the lateral surface of the incubation module in the length direction, so that the chip in the sample injection module is conveniently shifted into the chip incubation groove, and the chip in the chip incubation groove is also conveniently shifted into the cleaning module; similarly, the NC film Wen Yocao freely slides along the polished rod of the NC film incubation groove, and the sample injection module, the cleaning module and the NC film detection module are arranged along the lateral surface of the length direction of the incubation module, so that NC film clamping strips can be conveniently poked into the NC film incubation groove, and NC film clamping strips in the NC film incubation groove can also be conveniently poked into the NC film detection module; the groove position can be provided with a plurality of chips or NC membrane card strips for synchronous detection, so that the synchronous transmission of the chips and NC membrane card strips in the system can be met, and the requirements of the detection system on flux and efficiency are met.

Preferably, the cleaning module is provided with a chip cleaning tank, the chip cleaning tank is fixedly connected with a chip cleaning transmission connecting block, the chip cleaning transmission connecting block is fixedly arranged on a synchronous belt driven by a chip cleaning tank transmission control motor, and the chip cleaning tank moves back and forth on a linear guide rail; the cleaning module is controlled by a cleaning liquid path control motor, and the cleaning liquid path control motor is arranged above the cleaning module.

In the invention, the cleaning module is in a specific structure, the cleaning module is the same as the liquid path gas path module, the cleaning module is controlled by the cleaning liquid path control motor to press down, the cleaning module adopts the same structural design (gas path sealing piece) as the liquid path gas path module to ensure the air tightness, the cleaning is carried out by pumping the cleaning liquid at positive pressure by a liquid pump, and the cleaning liquid is pumped for a plurality of times each time, and the cleaning liquid in the reaction area of the chip is emptied by pumping the positive pressure gas by an air pump until the cleaning is completed; the gas-liquid same-way mode is adopted, and a sample inlet and valve pump control system is utilized, and the special requirement of the microfluidic chip on gas-liquid sample injection can be met by combining a microcomputer control time sequence.

Preferably, the NC film detection module includes an NC film detection light path, an NC film detection card slot is disposed below the NC film detection light path, and the NC film detection light path is controlled to move above the NC film detection card slot by an NC film detection control motor; the side of NC membrane detection draw-in groove is provided with spacing post, is controlled by abandoning card strip control motor NC membrane detection draw-in groove removes.

After NC film clamping strips are shifted into NC film detection clamping grooves from NC film incubation grooves, an NC film detection control motor controls NC film detection light paths to move forwards and backwards above the NC film detection clamping grooves to collect signals, analysis is carried out, after detection is finished, the NC film detection clamping grooves are controlled by a discarding clamping strip control motor to move rightwards, limiting columns are arranged on the side faces of the NC film detection clamping grooves to limit the NC film clamping strips to move rightwards, and when the NC film detection clamping grooves move to a certain position, the NC film clamping strips drop the NC film detection clamping grooves to finish the action of discarding the clamping strips.

Preferably, the NC film card strip is pulled from the NC film incubation groove into the NC film detection card groove by an NC film sample pulling hand on the NC film sample pulling detection member.

Preferably, the chip detection module comprises an excitation light module, an emission light receiving port is arranged below the excitation light module, a light shielding plate is driven by a light shielding plate driving motor to move, and the light shielding plate is arranged below the emission light receiving port; a photomultiplier PMT is also arranged at the bottom of the chip detection module; the shielding plate is arranged to prevent the interference of the side light source on detection and improve the sensitivity of optical detection.

Preferably, the output shaft of the light shielding plate driving motor is connected with a rotary table, the rotary table is provided with a poking column, and the poking column is inserted into the groove of the light shielding plate.

The rotation of the turntable drives the poking column on the turntable to move, so that the light shielding plate moves left and right.

Drawings

The following is a further detailed description of embodiments of the invention with reference to the accompanying drawings:

FIG. 1 is a schematic diagram of the device structure of the integrated machine for multi-flux detection of a microfluidic chip and an NC membrane;

FIG. 2 is a schematic diagram of the sample injection module in FIG. 1;

FIG. 3 is a schematic diagram of a rear view of the sample injection module in FIG. 2;

FIG. 4 is a schematic diagram of a liquid path and gas path module structure in the present invention;

FIG. 5 is a schematic view of the gas circuit seal of FIG. 4;

FIG. 6 is a schematic view of the structure of the incubation module of FIG. 1;

FIG. 7 is a schematic view of the cleaning module of FIG. 1;

FIG. 8 is a schematic diagram of the NC film detection module in FIG. 1;

FIG. 9 is a schematic diagram of a chip detection module according to the present invention;

FIG. 10 is an exploded view of FIG. 2;

FIG. 11 is an exploded view of FIG. 3;

FIG. 12 is an exploded view of FIG. 4;

wherein: 1-a base; 2-a sample injection module; 201-a chip sample introduction clamping groove; 202-NC membrane sample introduction clamping groove; 203-chip sample introduction shifting; 204-a first drive connection block; 205-chip sample injection manual force motor; 206-NC film sample injection shifting; 207-a second drive connection block; 208-NC film sample injection shifting manual motor; 209-a first drive slide; 2010-a second drive slide; 2011-chip sample injection clamping groove control motor; 2012-NC membrane sample feeding clamping groove control motor; 3-incubation module; 301-chip Wen Yocao; 302-NC membrane incubation tank; 303-NC membrane incubation tank control motor; 304-chip incubation tank control motor; 305-chip Wen Yocao polish rod; 306-NC membrane incubation groove polish rod; 307-drive slide; 308-a bracket; 309-slide out; 4-a cleaning module; 401-chip cleaning tank; 402-chip cleaning transmission connection block; 403-chip cleaning tank transmission control motor; 404-linear guide rail; 405-a cleaning fluid path control motor; a 5-NC film detection module; 501-NC film detection light path; 502-NC film detection clamping groove; 503-NC film detection control motor; 504-a limit column; 505-discard card strip control motor; 506-a detection platform; 507-a blocking piece; 508-NC film card strip placement area; 509-NC membrane card strips fall into the port; 5010—a surrounding edge; 5011-a limit column hole; 5012-NC film detection slide block; 6-a chip detection module; 601-an excitation light module; 602-connecting an emission light port; 603-a visor drive motor; 604-a mask; 605-photomultiplier PMT; 606-an output shaft; 607-turntable; 608-toggle the column; 609-groove; 7, a liquid path gas path module; 701-a liquid path gas path module controls a motor; 702-upper and lower sliders; 703-sample injection gas circuit column; 704-a liquid path column; 705—a liquid level bar; 706-a valve plunger; 707-gas path seal; 708-a seal ring; 709-valve control motor; 7010-upper rack; 7011-a lower rack; 7012. 7018-a lead screw nut; 7013-an upper facing layer; 7014-a lower facing layer; 7015-notch; 7016. 7017-a screw rod; 7019-lightening holes; 7020, a guide rail; an 8-NC membrane sample-out detection member; 801-NC film sample-discharging hand.

Detailed Description

The device for the microfluidic chip and NC membrane multi-flux detection integrated machine is shown in FIG. 1, and is structurally schematic, and comprises a base 1, wherein a sample injection module 2, an incubation module 3, a cleaning module 4, an NC membrane detection module 5 and a chip detection module 6 are arranged on the base 1;

wherein, the sample injection module 2 is provided with a chip sample injection clamping groove 201 and an NC film sample injection clamping groove 202 which are mutually independent and are respectively used for inserting a chip or NC film clamping strip; the sample injection module 2 is also provided with a liquid path gas path module 7;

the incubation module 3 is provided with a chip Wen Yocao and an NC membrane incubation groove 302 which are mutually independent, and the sample injection module 2, the cleaning module 4 and the NC membrane detection module 5 are arranged along the side surface of the incubation module 3 in the length direction; the chip cleaned in the cleaning module 4 is sent to the chip detection module 6 for detection.

As shown in fig. 2, 3, 10 and 11, a chip sample introduction pulling hand 203 is arranged on the side surface of the chip sample introduction clamping groove 201, the chip sample introduction pulling hand 203 is fixedly connected to a first transmission connection block 204, and the first transmission connection block 204 is driven by a chip sample introduction pulling hand force motor 205 to move back and forth through a synchronous belt; an NC film sample introduction hand 206 is disposed on the side of the NC film sample introduction card slot 202, the NC film sample introduction hand 206 is fixedly connected to a second transmission connection block 207, and the second transmission connection block 207 is driven by the NC film sample introduction hand power motor 208 to move back and forth through a synchronous belt.

A first transmission sliding bar 209 is arranged below the first transmission connecting block 204; a second transmission slide 2010 is arranged below the second transmission connecting block 207; the chip sample feeding clamping groove 201 is controlled by a chip sample feeding clamping groove control motor 2011, the NC film sample feeding clamping groove 202 is controlled by an NC film sample feeding clamping groove control motor 208, and the liquid path gas path module 7 is controlled by a liquid path gas path module control motor 701 to act.

As shown in fig. 4, the liquid path module 7 includes an upper slider 702, a lower slider 702, a sample injection gas path column 703, a liquid path column 704, a liquid level rod 705, and a valve pressure rod 706; the end of the sample injection gas path column 703 is provided with a gas path sealing member 707, the gas path sealing member 707 is provided with a sealing ring 708 (as shown in fig. 5), in this embodiment, the sealing ring 708 is made of rubber, so as to realize soft contact, a semicircular arc of the sealing ring 708 adopts a multi-channel rib bridge structure, and the rib bridge is extruded and deformed, so that a sealing effect is started; the valve pressing rod 706 is controlled by a valve control motor 709, and the valve pressing rod control motor 709 and the liquid path gas path module control motor 701 are both arranged above the liquid path gas path module 7.

In this embodiment, the end of the liquid column 704 is also provided with a gas path sealing member 707, and a sealing ring 708 is also installed on the gas path sealing member 707; in addition, regarding the liquid path gas path module 7 of the embodiment, specifically, an upper bracket 7010, a valve pressure lever control motor 709 and a liquid path gas path module control motor 701 are all arranged on the upper bracket 7010, a lower bracket 7011 is movably connected with the upper bracket 7010 through an upper sliding block 702 and a lower sliding block 702, and a guide rail 7020 matched with the upper sliding block 702, wherein, a sample injection gas path column 703, a liquid path column 704 and a liquid level rod 705 are all arranged on the lower bracket 7011, the liquid path gas path module control motor 701 is a screw rod motor, a screw rod nut 7012 matched with a screw rod 7016 on the liquid path gas path module control motor 701 is fixedly arranged on the lower bracket 7011, the lower bracket 7011 is driven to slide up and down, the lower bracket is divided into two layers, namely an upper end surface layer 7013 and a lower end surface layer 7014, the upper end surface layer 7013 is used for installing the screw rod nut 7012, a notch 7015 is also arranged on the upper end surface layer 7013, the screw rod nut 7018 matched with the valve control motor 709 is arranged at a mounting position, the valve pressure lever 706 is movably inserted in the lower end surface layer 7011, the sample injection gas path column is also connected with the liquid path column 703, and the sample injection gas path column is connected with the liquid path column 70703; fig. 12 is an exploded view of the above-mentioned liquid path and gas path module 7, and it can be seen from the figure that the liquid path and gas path module 7 has a more detailed structure, and the upper bracket 7010 is further provided with a weight-reducing hole 7019.

In the incubation module 3, as shown in fig. 6, the chip Wen Yocao 301 and NC film incubation groove 302 are arranged one above the other; the chip Wen Yocao has at least one slot for placement of a chip (in this embodiment, 8 slots), the chip Wen Yocao is controlled by a chip incubation slot control motor 304 such that the chip Wen Yocao is free to slide along the chip Wen Yocao polish rod 305; the NC membrane incubation groove 302 has at least one groove position for placing NC membrane cassettes (in this embodiment, 8 groove positions as well), and the NC membrane incubation groove 302 is controlled by an NC membrane incubation groove control motor 303 such that the NC membrane incubation groove 302 freely slides along an NC membrane incubation groove polish rod 306.

In the incubation module 3, a bracket 308 is provided, an NC film incubation groove 302 is positioned above, two NC film incubation groove polished rods 306 are provided, a synchronous belt installation space is reserved between the two NC film incubation groove polished rods 306, an NC film incubation groove control motor 303 is connected with a synchronous belt, a transmission sliding block 307 is arranged below the NC film incubation groove 302, and the transmission sliding block 307 is fixedly connected with the synchronous belt driven by the NC film incubation groove control motor 303; the chip Wen Yocao is located below the NC membrane incubation groove 302, and has the same structure as the NC membrane incubation groove 302, and in addition, a slide outlet 309 is formed on the right side of the bracket 308 and at a position corresponding to the chip Wen Yocao 301, so that the chip with the incubation time in the chip Wen Yocao 301 can be conveniently moved into the chip washing groove 401.

The cleaning module 4 is provided with a chip cleaning tank 401, the chip cleaning tank 401 is fixedly connected with a chip cleaning transmission connection block 402, the chip cleaning transmission connection block 402 is fixedly arranged on a synchronous belt driven by a chip cleaning tank transmission control motor 403, and the chip cleaning tank 401 moves back and forth on a linear guide rail 404; the cleaning module 4 is controlled by a cleaning solution path control motor 405, and the cleaning solution path control motor 405 is disposed above the cleaning module 4, as shown in fig. 7.

As shown in fig. 8, the NC film detection module 5 according to the present invention includes an NC film detection light path 501, an NC film detection card slot 502 is disposed below the NC film detection light path 501, and the NC film detection light path 501 is controlled to move above the NC film detection card slot 502 by an NC film detection control motor 503; the side of the NC film detection card slot 502 is provided with a limit post 504, and the movement of the NC film detection card slot 502 is controlled by a discard card bar control motor 505.

Specifically, the NC film detection module 5 includes a detection platform 506, the edge of the detection platform 506 is provided with a blocking piece 507, and the blocking piece 507 and the NC film detection card slot 502 enclose an NC film card strip placement area 508; an NC film card strip falling port 509 is arranged below the NC film card strip placing area 508 and positioned on the detection platform 506; the surrounding edge 5010 on the NC film detection clamping groove 502 is provided with a limit column hole 5011; the NC film detection light path 501 is fixedly connected with an NC film detection slider 5012, and the NC film detection slider 5012 is controlled to move by an NC film detection control motor 503, so as to drive the NC film detection light path 501 to move above the NC film detection card slot 502.

In fig. 1, we can also see that NC film cassettes are set from the NC film incubation slots 302 into the NC film detection cassettes 502 by NC film sample setting hands 801 on NC film sample setting detection members 8.

Fig. 9 shows a chip detection module 6, which includes an excitation light module 601, an emission receiving light port 602 is disposed below the excitation light module 601, a light shielding plate 604 is driven by a light shielding plate driving motor 603 to move, the light shielding plate 604 is disposed below the emission receiving light port 602, different optical filters can be mounted on the light shielding plate 604, and the light shielding plate driving motor 603 moves the light shielding plate 604 to receive different optical wavelengths; a photomultiplier tube PMT605 is also arranged at the bottom of the chip detection module 6, and a chip is arranged between the excitation light module 601 and the emission light receiving port 602; the output shaft 606 of the light shielding plate driving motor 603 is connected with a turntable 607, the turntable 607 is provided with a stirring column 608, and the stirring column 608 is inserted into the groove 609 of the light shielding plate 604.

The specific use is as follows:

(1) Detecting NC film card strips: inserting an NC film card strip into an NC film sample injection clamping groove 202, controlling the NC film sample injection clamping groove 202 to enter the device by an NC film sample injection clamping groove control motor 2012, reaching a preset position, driving an NC film sample injection hand 206 by an NC film sample injection hand power motor 208 to dial the NC film card strip into an NC film incubation groove 302 for constant temperature incubation, wherein the incubation time is completed, driving the NC film incubation groove control motor 303 to move the NC film incubation groove 302 to a specified position, dialing the NC film card strip from the groove position of the NC film incubation groove 302 into an NC film detection clamping groove 502 in an NC film detection module 5 by an NC film sample discharge hand 801, controlling an NC film detection light path 501 to move back and forth to acquire a signal, analyzing, discarding the card strip, controlling the NC film detection clamping groove 502 to move rightwards by a limiting post 504 through a limiting post hole 5011 in a surrounding edge 5010 of the NC film detection clamping groove 502, limiting the NC film card strip to move rightwards, and dropping the NC film card strip from the NC film detection clamping groove 508 into an NC film detection clamping groove 509 after the detection is completed;

(2) Detecting the micro-fluidic chip: the chip with the added sample is inserted into the chip sample injection clamping groove 201, the chip sample injection clamping groove control motor 2011 controls the chip sample injection clamping groove 201 to enter the device to reach a preset position, the liquid path module control motor 701 controls the liquid path module 7 to be pressed down, sealing reliability of the matching position of the device (the liquid path module 7) and the chip is guaranteed, the liquid path column 704 seals a cleaning liquid port on the chip, the sample injection gas path column 703 presses a sample injection port on the chip, then the sample injection gas path column 703 blows up at positive pressure of the sample injection port to push the sample to reach a reaction area, at the moment, the liquid level rod 705 senses that the sample reaches and fills the reaction area, the valve control motor 709 immediately makes a closing valve response, the valve pressing rod 706 presses down the pressure dead valve to ensure that the sample and the reagent react in the chip reaction bin; the ultrasonic module (existing) at the bottom of the sample injection module 2 is contacted with the chip upwards for ultrasonic mixing, so that the full reaction of the reagent and the sample is ensured; the chip sample introduction pulling hand power motor 205 drives the chip sample introduction pulling hand 203 to pull the chip into the chip Wen Yocao for constant temperature incubation, and the incubation time is completed; the chip incubation groove control motor 304 drives the chip Wen Yocao to move to a designated position, the chip is moved into the chip cleaning groove 401, the chip cleaning groove transmission control motor 403 indirectly controls the chip cleaning groove 401 to move to the position right below the cleaning liquid path module, the cleaning liquid path control motor 405 controls the cleaning module 4 to press down, the cleaning module 4 adopts the same structural design as the liquid path gas path module 7 to ensure the air tightness, then, the liquid pump pumps the cleaning liquid to clean the chip in a positive pressure manner, the cleaning liquid is pumped for a plurality of times, each time the cleaning liquid is pumped, the air pump controls the blowing of the cleaning liquid in the positive pressure gas evacuation chip reaction area, the cleaning is completed, the chip cleaning groove 401 moves back to the chip detection module 6 to perform optical path collection processing (the chip is placed between the excitation optical module 601 and the emission light receiving port 602), and the chip is thrown away after the data collection is completed.

The two detection steps can be carried out independently or simultaneously; in the embodiment, a micro-fluidic chip detection flow monitoring system can be adopted to monitor the real-time reaction process and detection time of the micro-fluidic chip in real time, so that the user can know the detection progress, the system can automatically distribute the detection management files of the chip at the first time when the chip enters an instrument to start detection, and the chip two-dimensional code is used to know the chip items and the parameter curves, so that the chip measurement time and the residual detection time are displayed, and the user can conveniently master the detection progress in real time; consumable materials generated in the detection process of the NC film and the microfluidic chip are automatically discarded separately and stored in a classified manner; the double-area sampling mode improves the detection precision and stability, and skillfully meets the requirements of the micro-fluidic chip on the sampling precision and stability.

The foregoing has outlined and described the basic principles, features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the foregoing embodiments, which have been described in the foregoing and disclosure merely as illustrative of the principles of the invention, and various changes and modifications may be made in the location of various components mounted on a base without departing from the spirit and scope of the invention, and such changes and modifications fall within the scope of the invention as hereinafter claimed. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. The sample injection module for the multi-flux detection of the microfluidic chip and the NC membrane is characterized in that a chip sample injection clamping groove and an NC membrane sample injection clamping groove which are mutually independent are arranged in the sample injection module and are respectively used for inserting a chip or an NC membrane clamping strip; the sample injection module is also provided with a liquid path and gas path module;

a chip sample injection shifting hand is arranged on the side surface of the chip sample injection clamping groove and fixedly connected to a first transmission connecting block, and the first transmission connecting block is driven by a chip sample injection shifting hand force motor to move back and forth through a synchronous belt; an NC film sample introduction hand is arranged on the side surface of the NC film sample introduction clamping groove and fixedly connected to a second transmission connecting block, and the second transmission connecting block is driven by an NC film sample introduction hand power motor to move back and forth through a synchronous belt;

a first transmission sliding bar is arranged below the first transmission connecting block; a second transmission sliding bar is arranged below the second transmission connecting block; the chip sample injection clamping groove is controlled by a chip sample injection clamping groove control motor, the NC film sample injection clamping groove is controlled by an NC film sample injection clamping groove control motor, and the liquid path gas path module is controlled by a liquid path gas path module control motor to act;

the liquid path gas path module comprises a sample injection gas path column, a gas path sealing piece is arranged at the end part of the sample injection gas path column, a sealing ring is arranged on the gas path sealing piece, and the sealing ring is made of rubber.

2. The sample injection module for the multi-flux detection of the micro-fluidic chip and the NC membrane according to claim 1, wherein the liquid path gas path module further comprises an upper sliding block, a lower sliding block, a liquid path column, a liquid level rod and a valve pressing rod; the valve pressing rod is controlled by a valve control motor, and the valve pressing rod control motor and the liquid path gas path module control motor are both arranged above the liquid path gas path module.

3. The sample injection module for multi-flux detection of the microfluidic chip and the NC membrane according to claim 2, wherein a multi-channel rib bridge structure is adopted on the semicircular arc of the sealing ring.

4. The sample injection module for multi-flux detection of a microfluidic chip and an NC membrane according to claim 3, wherein the liquid path gas path module has an upper bracket; the valve pressing rod control motor and the liquid path module control motor are both arranged on the upper bracket; the lower support is movably connected with the upper support through the upper and lower sliding blocks, wherein the sample injection gas circuit column, the liquid circuit column and the liquid level rod are all arranged on the lower support.

5. The sample injection module for the multi-flux detection of the micro-fluidic chip and the NC membrane according to claim 4, wherein the liquid path gas path module control motor is a screw rod motor, and a screw rod nut matched with a screw rod on the liquid path gas path module control motor is fixedly arranged on the lower bracket to drive the lower bracket to slide up and down.

6. The sample injection module for multi-flux detection of a microfluidic chip and an NC membrane according to claim 5, wherein the lower support is divided into two layers, an upper end surface layer and a lower end surface layer, the upper end surface layer is used for mounting the screw nut, and a notch is further formed on the upper end surface layer; the valve compression bar is movably inserted in the lower end surface layer, the liquid path column is also connected with a liquid pump, and the sample injection gas path column is also connected with an air pump.

7. The sample injection module for multi-flux detection of a microfluidic chip and an NC membrane according to claim 6, wherein the upper bracket is further provided with a lightening hole.