CN106226540A - Full-automatic protein chip analyzer - Google Patents
Full-automatic protein chip analyzer Download PDFInfo
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- CN106226540A CN106226540A CN201610789335.5A CN201610789335A CN106226540A CN 106226540 A CN106226540 A CN 106226540A CN 201610789335 A CN201610789335 A CN 201610789335A CN 106226540 A CN106226540 A CN 106226540A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/21—Polarisation-affecting properties
- G01N21/211—Ellipsometry
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/0099—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor comprising robots or similar manipulators
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/025—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a carousel or turntable for reaction cells or cuvettes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
- G01N2035/00099—Characterised by type of test elements
- G01N2035/00158—Elements containing microarrays, i.e. "biochip"
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
- G01N2035/0401—Sample carriers, cuvettes or reaction vessels
- G01N2035/0437—Cleaning cuvettes or reaction vessels
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Abstract
The invention provides a full-automatic protein chip analyzer, which belongs to the field of research equipment and comprises a rack, a sample container, an observation system, a reaction system, an electric control system and a motion system, wherein the observation system, the reaction system, the motion system and the electric control system are all connected with the rack, the sample container is used for containing a sample, the sample is used for contacting with a reactor and reacting, the rack comprises a workbench, the reaction system comprises a reaction table, the reaction table is connected with the workbench, the motion system and the reaction system are all electrically connected with the electric control system, the reaction system comprises the reactor, the observation system is used for observing the reaction condition of the reactor, the motion system comprises a mechanical arm used for carrying out experimental operation, the mechanical arm is used for transferring the reactor to the reaction table, and the mechanical arm is also used for grabbing a disposable suction head and adding the sample into the reactor. The device changes the steps of protein analysis into automation, reduces the probability of cross infection in the test, and improves the efficiency and accuracy of the test.
Description
Technical field
The present invention relates to study clothing arts, in particular to full-automatic ProteinChip Analysis instrument.
Background technology
At present, the detection technique in medical institutions is divided mainly by euzymelinked immunosorbent assay (ELISA), Full-automatic chemiluminescence immunity
The traditional immunization determination techniques of analysis method carries out single index detection.Enzyme linked immunological law technology mainly by adding model, ovum educates device, enzyme mark
Instrument, wash trigger, data processing module composition.The ultimate principle of this technology is: 1. make antigen or antibodies carry to certain solid phase
Surface, and keep its immunocompetence.2. making antigen or antibody and certain enzyme connect into enzyme-labelled antigen or antibody, this enzyme mark resists
Former or antibody had both retained its immunocompetence, retained again the activity of enzyme.Measure time, by examine specimen (measure antibody therein or
Antigen) and enzyme-labelled antigen or antibody react by antigen or the antibody of different steps with surface of solid phase carriers.Side with washing
Method make on solid phase carrier formed antigen antibody complex separate with other materials, the enzyme amount being finally combined on solid phase carrier and
In specimen, the amount of tested substance becomes certain ratio.After adding the substrate of enzyme reaction, substrate is become color products by enzyme catalysis, produces
The amount of thing is directly related with the amount of tested substance in specimen, therefore qualitatively or quantitatively can divide according to the depth of color reaction
Analysis.Chemiluminescent ultimate principle is molecule (reactant, product, intermediate or the fluorescence of certain material in reaction system
Matter) absorb energy that reaction discharged and by ground state transition to excited state, return ground state from excited state the most again, simultaneously by energy
Amount discharges with the form of light radiation, produces chemiluminescence, typically by sample disk, reagent disc, incubation reaction dish, electrochemistry inspection
Examining system, computer control system form.
Inventor finds under study for action, and existing technology is complicated to the requirement of reagent due to labelling, and complex operation step is fixed
Amount difficulty, its labelling easily causes cross-contamination, and multinomial marker detection then needs long loaded down with trivial details work, and higher taking
With, therefore be difficult to promote.
Summary of the invention
The invention provides a kind of full-automatic ProteinChip Analysis instrument, one of its purpose is, reduces protein analysis
The manual operation of experiment；The two of its purpose are, it is to avoid the cross-contamination produced in experimentation；The three of its purpose are, contracting
The time of short protein analysis experiment, improve the efficiency of experiment.
The present invention is achieved in that
A kind of full-automatic ProteinChip Analysis instrument, including frame, shuttle, viewing system, response system, automatically controlled system
Uniting and motor system, described viewing system, response system, motor system and electric-control system are all connected with described frame, described sample
Product container is used for accommodating sample, and described sample is for contacting with described reactor and reacting, and described frame includes workbench,
Described response system includes that reaction bench, described reaction bench are connected with described workbench, described motor system and described response system
All electrically connecting with described electric-control system, described response system includes that reactor, described viewing system are used for observing described reactor
Response situation, described motor system includes the mechanical arm for carrying out experimental implementation, and described mechanical arm is for by described reaction
Described reaction bench transferred to by device, and described frame also includes that suction nozzle box, described suction nozzle box are used for placing disposable tip, described machinery
Arm is additionally operable to capture described disposable tip and add described sample in described reactor.
In a preferred embodiment of the invention, described frame includes that guide rail, described mechanical arm are connected with described slide,
Described electric-control system is used for controlling described mechanical arm along described slide.
In a preferred embodiment of the invention, described mechanical arm includes first and second, described first with described
Slide connects, and described first includes X direction guiding rail, and described X direction guiding rail is arranged along the short transverse of described frame, and described the
Two are slidably connected with described X direction guiding rail, and described electric-control system is additionally operable to drive described second to slide along described X direction guiding rail.
In a preferred embodiment of the invention, described analyser also includes rotating disk and suction nozzle box, described suction nozzle box and institute
Stating workbench or described frame connects, described suction nozzle box has deposited multiple disposable tip, described rotating disk and described workbench
Being rotationally connected, the top of described rotating disk is placed with at least one described shuttle and at least one reactor, described automatically controlled
System is additionally operable to drive described rotating disk to rotate, and described mechanical arm is additionally operable to capture described disposable tip to extract described sample
Product, and described sample is transferred to described reactor, the direction, rotary centerline place of described rotating disk and the height of described frame
Degree direction is parallel.
In a preferred embodiment of the invention, described workbench is additionally provided with for concentrating losing of the disposable tip used up
Abandoning mouth, described frame also includes discarded collecting box, and described discarded collecting box is positioned at the bottom of described workbench, described disposable suction
Head can fall into described discarded collecting box from the described mouth that abandons.
In a preferred embodiment of the invention, described frame also includes benchmark table top and matter storage lattice, and described matter storage lattice is arranged
In the bottom of described benchmark table top, described workbench is arranged on the top of described benchmark table top.
In a preferred embodiment of the invention, described reactor includes chip and the reactor body removably connected, institute
The surface configuration stating chip has active organism probe, described active organism probe to be set to multiple array, each biological activity
The all corresponding protein array micro unit of probe, described active organism probe is for combining target molecule and generates biomolecule
Complex, described viewing system is for observing the change procedure of active organism probe.
In a preferred embodiment of the invention, described analyser also includes the turning device for overturning described chip, institute
Stating turning device to be connected with described reaction bench, described turning device includes rotating movable part, in the rotation of described movable part
Heart line level, described chip can rotate 180 ° by described turning device.
In a preferred embodiment of the invention, described viewing system includes observation platform, oval thickness assembly and CCD shooting
Machine, described observation platform is connected with described frame, described observation platform reactor after placing response, described oval thickness assembly
For extending polarized lightwave and irradiating described reactor, described ccd video camera is due to the sample letter being loaded with in detection reflecting light
Breath also stores to generate the form of digital picture.
In a preferred embodiment of the invention, described analyser also includes the clear of the surface residue for purge reactor
Clean system, described cleaning systems electrically connect with described electric-control system, and described cleaning systems include rinsing table, nitrogen machine, conduit, note
Penetrating pump and soda liquor container, one end of described conduit connects with described soda liquor container, and the other end of described conduit points to described
Rinsing table, described syringe pump for moving the cleanout fluid in described soda liquor container and rinse described rinsing table, institute along conduit
Stating rinsing table to be connected with described workbench, described nitrogen machine is for drying up the reactor being positioned on described observation platform, described machinery
Arm is additionally operable to move to reacted reactor the roof of described rinsing table and is rinsed, and will rinse after reactor be placed in
Described observation platform.
The invention has the beneficial effects as follows: utilize electric-control system to drive motor system 200 to carry out experimental implementation, in motor system
Mechanical arm replace manually testing, and by viewing system, reactor is studied, alleviates artificial work.Number
The control of controlization can effectively reduce the mistake that manual operation error may be brought, and improves the accuracy of experimental result, accelerates real
The efficiency tested.
Accompanying drawing explanation
In order to be illustrated more clearly that the technical scheme of the embodiment of the present invention, below by embodiment required use attached
Figure is briefly described, it will be appreciated that the following drawings illustrate only certain embodiments of the present invention, and it is right to be therefore not construed as
The restriction of scope, for those of ordinary skill in the art, on the premise of not paying creative work, it is also possible to according to this
A little accompanying drawings obtain other relevant accompanying drawings.
The overall schematic of a kind of full-automatic ProteinChip Analysis instrument that Fig. 1 provides for the embodiment of the present invention 1；
Fig. 2 is the front view of Fig. 1；
Fig. 3 is the rearview of Fig. 1；
Fig. 4 is the top view of Fig. 1；
The front view of the reactor that Fig. 5 provides for the embodiment of the present invention 2；
Fig. 6 is the upward view of Fig. 5；
Fig. 7 is the sectional view of Fig. 5；
Fig. 8 is the partial schematic diagram of Fig. 7；
The structural representation of the reactor that Fig. 9 provides for the embodiment of the present invention 3；
Figure 10 is the left view of Fig. 9；
The structural representation of the reactor that Figure 11 provides for the embodiment of the present invention 4；
The structural representation of the reactor that Figure 12 provides for the embodiment of the present invention 5.
Reference collects:
Reactor 100；Chip carrier 101；Chip 102；First reaction film 103；Reactor body 104；Projection 105；Second
Reaction film 106；Capture hole 107；Drainage lumens 109；Feed pathway 110；Liquid outlet channel 111, the first feeding-passage 112；Second is anti-
Answer passage 113；Reaction bench 114；Peristaltic pump 115；Shuttle 200；Sample 210；Viewing system 300；Observation platform 310；Ellipse partially
Optical module 320；Ccd video camera 330；Frame 400；Guide rail 410；Benchmark table top 420；Matter storage lattice 430；Workbench 440；Abandon
Mouth 441；Discarded collecting box 450；Electric-control system 500；Motor system 600；Mechanical arm 610；First 611；X direction guiding rail 612；The
Two 613；Rotating disk 700；Suction nozzle box 800；Disposable tip 810；Turning device 900；Movable part 901；Cleaning systems 1000；
Clean hatch 1010.
Detailed description of the invention
Existing technology is complicated to the requirement of reagent due to labelling, and complex operation step, difficult quantitation, its labelling is easily made
Becoming cross-contamination, multinomial marker detection then needs long loaded down with trivial details work, and higher expense, therefore is difficult to promote.
In consideration of it, the invention provides a kind of full-automatic ProteinChip Analysis instrument, each by Automatic Control is
System, eliminates manual operation, improves the efficiency of experiment.
It should be noted that in the case of not conflicting, the embodiment in the present invention and the feature in embodiment can phases
Combine mutually.
For making the purpose of the embodiment of the present invention, technical scheme and advantage clearer, below in conjunction with the embodiment of the present invention
In accompanying drawing, the technical scheme in the embodiment of the present invention is clearly and completely described, it is clear that described embodiment is
The a part of embodiment of the present invention rather than whole embodiments.Generally implement with the present invention illustrated described in accompanying drawing herein
The assembly of example can be arranged with various different configurations and design.Therefore, reality to the present invention provided in the accompanying drawings below
The detailed description executing example is not intended to limit the scope of claimed invention, but is merely representative of the selected enforcement of the present invention
Example.Based on the embodiment in the present invention, those of ordinary skill in the art are obtained under not making creative work premise
Every other embodiment, broadly falls into the scope of protection of the invention.
In describing the invention, it is to be understood that term " " center ", " longitudinally ", " laterally ", " length ", " width ",
" thickness ", " on ", D score, "front", "rear", "left", "right", " vertically ", " level ", " top ", " end " " interior ", " outward ", " up time
Pin ", the orientation of the instruction such as " counterclockwise " or position relationship be based on orientation shown in the drawings or position relationship, or this invention
The orientation usually put or position relationship when product uses, or the orientation that usually understands of those skilled in the art or position close
System, be for only for ease of the present invention rather than instruction or the equipment of hint indication described or element must have specific orientation,
With specific azimuth configuration and operation, therefore it is not considered as limiting the invention.
Additionally, term " first ", " second " are only used for describing purpose, and it is not intended that instruction or hint relative importance
Or the implicit quantity indicating indicated technical characteristic.Thus, define " first ", the feature of " second " can express or
Implicitly include one or more this feature.In describing the invention, " multiple " are meant that two or more,
Unless otherwise expressly limited specifically.
In the present invention, unless otherwise clearly defined and limited, term " install ", " being connected ", " connection ", " fixing " etc.
Term should be interpreted broadly, and connects for example, it may be fixing, it is also possible to be to removably connect, or integral；Can be that machinery connects
Connect, it is also possible to be electrical connection；Can be to be joined directly together, it is also possible to be indirectly connected to by intermediary, in can being two elements
The connection in portion or the interaction relationship of two elements.For the ordinary skill in the art, can be according to concrete feelings
Condition understands above-mentioned term concrete meaning in the present invention.
In the present invention, unless otherwise clearly defined and limited, fisrt feature second feature it " on " or D score
Can include that the first and second features directly contact, it is also possible to include that the first and second features are not directly contact but by it
Other characterisation contact between.And, fisrt feature second feature " on ", " top " and " above " include that first is special
Levy directly over second feature and oblique upper, or be merely representative of fisrt feature level height higher than second feature.Fisrt feature exists
Second feature " under ", " lower section " and " below " include that fisrt feature immediately below second feature and obliquely downward, or is merely representative of
Fisrt feature level height is less than second feature.
Embodiment 1
Referring to Fig. 1-Fig. 4, the embodiment of the present invention 1 provides a kind of full-automatic protein chip 102 analyser, including machine
Frame 400, shuttle 200, rotating disk 700, turning device 900, cleaning systems 1000, viewing system 300, response system, electricity
Ore-controlling Role 500 and motor system 600.
Viewing system 300, cleaning systems 1000, rotating disk 700, turning device 900, response system, motor system 600 and
Electric-control system 500 is all connected with frame 400.
Frame 400 includes workbench 440, and response system includes that reaction bench 114, reaction bench 114 are connected with workbench 440,
Motor system 600 and response system all electrically connect with electric-control system 500, and response system includes reactor 100, viewing system 300
For the response situation of observing response device 100, motor system 600 includes the mechanical arm 610 for carrying out experimental implementation, mechanical arm
610 for transferring to reaction bench 114 by reactor 100, and frame 400 also includes suction nozzle box 800, and suction nozzle box 800 is for placement one
Secondary property suction nozzle 810, shuttle 200 is used for accommodating sample 210, and sample 210 is used for contacting with reactor 100 and reacting,
Mechanical arm 610 is additionally operable to capture disposable tip 810 and add sample 210 in reactor 100.
Frame 400 includes that guide rail 410, mechanical arm 610 are slidably connected with guide rail 410, and electric-control system 500 is used for controlling machinery
Arm 610 slides along guide rail 410.
In order to realize the motion of mechanical arm 610 in the vertical direction, the mechanical arm 610 in the present embodiment includes first
611 and second 613, first 611 is slidably connected with guide rail 410, and first 611 includes X direction guiding rail 612, X direction guiding rail 612
Short transverse along frame 400 is arranged, and second 613 is slidably connected with X direction guiding rail 612, and electric-control system 500 is additionally operable to drive the
Slide along X direction guiding rail 612 for two 613.
Suction nozzle box 800 is connected with workbench 440 or frame 400, and multiple disposable tip 810 deposited by suction nozzle box 800, rotation
Rotating disk 700 is rotationally connected with workbench 440, the top of rotating disk 700 be placed with at least one shuttle 200 and at least one
Reactor 100, electric-control system 500 is additionally operable to drive rotating disk 700 to rotate, and mechanical arm 610 is additionally operable to capture disposable tip 810
To extract sample 210, and sample 210 is transferred to reactor 100, the direction, rotary centerline place of rotating disk 700 and frame
The short transverse of 400 is parallel.
Utilize the rotational motion of rotating disk 700, the position of the shuttle 200 being positioned in rotating disk 700 can be adjusted.Logical
Cross the manner, multiple shuttle 200 can be provided above in rotating disk 700.Owing to mechanical arm 610 slides along guide rail 410,
Therefore, the mobile route of mechanical arm 610 is fixed.When arranging many bottles of shuttles 200 in rotating disk 700, can be by automatically controlled
System 500 drives rotating disk 700 to rotate so that shuttle 200 moves the lower section to mechanical arm 610, in order to mechanical arm 610 carries
Take sample 210.
Workbench 440 be additionally provided with for concentrate use up disposable tip 810 abandon mouth 441, frame 400 also includes
Discarded collecting box 450, discarded collecting box 450 is positioned at the bottom of workbench 440, and disposable tip 810 can fall from abandoning mouth 441
Enter discarded collecting box 450.
Frame 400 also includes benchmark table top 420 and matter storage lattice 430, and matter storage lattice 430 is arranged on the bottom of benchmark table top 420,
Workbench 440 is arranged on the top of benchmark table top 420.Matter storage lattice 430 can be used not only for storing the article that experiment is relevant, also profit
With the space of frame 400, improve benchmark table top 420 and the height of workbench 440, also provide space to recovery system.
Reactor 100 includes chip 102 and the reactor body 104 removably connected, and the surface configuration of chip 102 has life
Thing active probe, active organism probe is set to multiple array, all corresponding protein array of each active organism probe
Micro unit, active organism probe is for combining target molecule and generates bimolecular complexes, and viewing system 300 is used for observing life
The change procedure of thing active probe.Chip 102 can be separated by mechanical arm 610 with reactor body 104.
When sensitive surface contacts with the solution containing target molecule, if the target molecule in solution and joining on probe
Base molecule occurs specific binding, will generate bimolecular complexes, and surface of solids biomolecule surface density changes, this
Change can be observed by oval thickness imaging system high-resolution.
Turning device 900 is for turning-over of chip 102, and turning device 900 is connected with reaction bench 114, and turning device 900 includes
Rotating movable part 901, the rotary centerline level of movable part 901, chip 102 can be rotated by turning device 900
180°。
Turning device 900 turning-over of chip 102 so that the end face of the multielement protein chip 102 of tabular and bottom surface exchange.Upset
Meaning be, virtual condition when chip 102 is separated from reactor 100 and the chip 102 reason on observation platform 310
Thinking that state is different, without through upset, the information that viewing system 300 can be caused to obtain is inaccurate.Inventor is the most directly
Change reaction environment and the layout of viewing system 300 of reactor 100, but convert many by the turning device 900 added
The angle of unit's protein chip 102, in order to observing response result.
In a preferred embodiment of the invention, viewing system 300 includes observation platform 310, oval thickness assembly 320 and CCD
Video camera 330, observation platform 310 is connected with frame 400, the observation platform 310 reactor 100 after placing response, oval thickness
Assembly 320 is used for extending polarized lightwave and irradiating reactor 100, and ccd video camera 330 is due to the sample being loaded with in detection reflecting light
Product 210 information also stores to generate the form of digital picture.
Ccd video camera 330 refers to the video camera with CCD chip 102.CCD refers to charge-coupled image sensor.Ellipse by imaging
The inclined technology of circle, research worker is capable of the ellipsometry analysis under extra small piece of film analysis, in situ ellipsometry, various liquid environment
And can realize being combined with multiple technologies.When being utilized to the combination studying albumen, experimenter is without as fluorescent immune method, enzyme
Linked immunosorbent assay and radioimmunology use the second antibody reagent of labelling like that to the biomolecule detected, and divide biology to be measured
Son activity undisturbed and damage.Additionally, detection is highly sensitive, the biological membranous layer change of 0.1nm can be identified.
In the present embodiment, cleaning systems 1000 electrically connect with electric-control system 500, cleaning systems 1000 include rinsing table,
Nitrogen machine, conduit, syringe pump and soda liquor container, one end of conduit connects with soda liquor container, and the other end of conduit points to clear
Washing platform, syringe pump is for moving by the cleanout fluid in soda liquor container along conduit and rinse rinsing table, rinsing table and workbench 440
Connecting, nitrogen machine is for drying up the reactor 100 being positioned on observation platform 310, and mechanical arm 610 is additionally operable to reacted reactor
100 roofs moving to rinsing table are rinsed, and will rinse after reactor 100 be placed in observation platform 310.
Additionally, further, in order to avoid the cross infection in experimentation, shuttle 200, disposable tip 810
It is disposable product with reactor 100.
During this device busy, first driven whether all parts unit meets testing conditions by electric-control system 500, then electricity
Ore-controlling Role 500 drives motor system 600 to work, and reactor 100 is transferred to reaction bench 114 by mechanical arm 610.Then mechanical arm
610 capture disposable tip 810 again and draw quantitative sample 210, are assigned to by sample 210 in enzyme connection box, then will disposably inhale
810 lose into abandoning mouth 441.Again PBST and deionized water are separately added in reactor 100.Response system includes peristaltic pump
115, electric-control system 500 drives response system to work, and the core of reactor 100 delivered to by sample 210 by peristaltic pump 115 as power source
On sheet 102.After chip 102 reacts, electric-control system 500 pioneer's motivation mechanical arm 610 by chip 102 from reactor 100 individually
Remove and be transferred to rinsing table, then drive cleaning systems 1000 to work, rinse out the residue on chip 102 surface, and by chip
102 dry up.Electric-control system 500 drives mechanical arm 610 to operate, and chip 102 is transferred to turning device 900.Electric-control system 500 drives
Dynamic turning device 900 motion upset 180 ° so that the end face of chip 102 and bottom surface exchange.After exchange completes, mechanical arm 610 is again
Chip 102 is moved to observation platform 310, viewing system 300 chip 102 is analyzed and data process.Finally, mechanical arm
The reactor body 104 of chip 102 and reactor 100 is abandoned to abandoning mouth 441 by 610, completes experiment or continues to start next
Secondary experiment.
As shown in Figure 4, in whole experimentation, complete the transfer of chip 102, turning device for the ease of mechanical arm 610
900, rotating disk 700, abandon mouth 441, reaction bench 114, observation platform 310, suction nozzle box 800 and clean hatch 1010 and be all located at mechanical arm
610 along the vertical lower section of the motion path of guide rail 410.
Embodiment 2
Referring to Fig. 5-Fig. 8, the embodiment of the present invention 2 provides a kind of reactor, including chip carrier the 101, first reaction film
103, reactor body 104 and for detecting the chip 102 of course of reaction.
Chip 102 is fixed on the bottom of chip carrier 101, and the roof of the first reaction film 103 contacts with the diapire of chip 102,
The roof of reactor body 104 and the diapire contact of the first reaction film 103, the main body of reactor is provided with feed pathway 110 He
Liquid outlet channel 111, feed pathway 110 and liquid outlet channel 111 all extend vertically through reactor body 104, the end of feed pathway 110
Portion for contacting with reactant liquor, the first reaction film 103 be provided with drainage lumens 109, feed pathway 110 and liquid outlet channel 111 all with
Drainage lumens 109 connects.
The liquid being carried out reactant liquor by the feed pathway 110 arranged and liquid outlet channel 111 is circulated, and the power of imbibition can
To be solved by external peristaltic pump.The drainage lumens 109 formed between reaction film with reactor body 104 is led to as connecting feed liquor
Road 110 and the exclusive path of liquid outlet channel 111 so that the reactant liquor flowing through drainage lumens 109 all can connect with the first reaction film 103
Touch so that produce reaction between the first reaction film 103 and reactant liquor, it is simple to observe, additionally, the stable reactant liquor introduced can make
The reaction obtained in drainage lumens 109 persistently occurs, it is simple to analyze.
In the preferred embodiment of the present invention, reactor also includes the second reaction film 106, the second reaction film 106 and reaction
The diapire laminating of device main body 104, liquid outlet channel 111 connects with the second reaction film 106.
Second reaction film 106 is used for carrying out another single step reaction so that this device can carry out many experiments, and second step
Reactant liquor is the reacted liquid through the first step.The mode of multistep reaction makes the in hgher efficiency of this device.
The first reaction film 103 and the second reaction film 106 in the present embodiment can be all PDMS film.PDMS material cost
Low, use simple, with having good adhesiveness between silicon chip, and there is good chemical inertness.
Reactor body 104 also includes that projection 105, projection 105 are arranged on the bottom of reactor body 104, feed pathway
110 run through projection 105.
The setting of projection 105 makes the bottom port of feed pathway 110 there is height with the bottom port of liquid outlet channel 111
Difference on direction, and then make when drawing reactant liquor, reactant liquor will not directly contact with the second reaction film 106, it is simple to rule
The path of model reactant liquor.Additionally, the projection 105 protruded also allows for being connected the container of feed pathway 110 with reactant liquor place.
Further, feed pathway 110 is 24, liquid outlet channel 111, drainage lumens 109 and the number of feed pathway 110 three
Amount is consistent, and each drainage lumens 109 connects a liquid outlet channel 111 and a feed pathway 110 simultaneously.The quantity of projection 105 is 4
Individual and 2 group of bumps of composition, each group of bumps all includes two projections 105, between two projections 105 in same group of bumps all
It is provided with second reaction film 106.
Improved by structure, improve the line density of reactant liquor, and make will not phase between different reactant liquor paths
Interference mutually, it is to avoid cross-contamination.In this case, the reaction efficiency of the reactant liquor under the control of this device is greatly improved.
The quantity of projection 105 increases, and the quantity of feed pathway 110 also is able to increase further.Controlling the second reaction film
Higher utilization rate can also be had under the quantity of 106.
Additionally, the total quantity of the quantity of group of bumps and feed pathway 110 can also be changed, carry out according to practical situation
Change.The situation of only one of which projection 105 or only one of which feed pathway 110 also is able to realize the purpose of the present invention.
In the present embodiment, chip 102 is micro-fluidic chip.Further, chip 102 is quartz or glass-chip, draws
The table wall in stream chamber is provided with the decorative layer by coupling agent bonding by organic polymer or protein.
Micro-fluidic chip has that liquid flowing is controlled, it is few to consume sample and reagent, analyze speed tenfold hundreds of times ground
Carrying high, it can be analyzed while carrying out up to a hundred samples within the most shorter time a few minutes, and can be
Line realizes the pretreatment of sample and analyzes overall process.Its target is the function whole laboratory, including sampling, dilute, adding
Reagent, react, separate, detection etc. is integrated on microchip, and can be used for multiple times.
Organic polymer mainly has polyvinyl alcohol, polyvinylacetate, hydroxyalkyl vinyl cellulose, Polyethylene Glycol, polyacrylamide
Amine, polysaccharide etc.；Protein such as bovine serum albumin.Decorative layer can promote the accuracy of chip detection reaction.
Additionally, chip 102 can also be plastic chip.In order to preferably cater to the demand of this device, chip can be carried out
Cement Composite Treated by Plasma, photo-irradiation treatment, hydrophilic process or hydrophobicity processes.
By this device, assembling and the association reaction of specificity one's share of expenses for a joint undertaking of aglucon can be carried out, implement sample transport, surface
The series of experiments steps such as formatting, aglucon assembling, surface clean, surface-closed and proteins react.
Reactor in the present embodiment is single use, is independent functional unit, can enter according to particular detection sample
Row targeted design, it is achieved detect while nine indexs of blood examination and tumor-marker, possesses reference cell and recessive comparison unit
Deng high flux reaction member.
Embodiment 3
Referring to Fig. 9 and Figure 10, the embodiment of the present invention 3 provides a kind of reactor, reacts including chip carrier 101, first
Film 103, reactor body 104, capture hole 107, peristaltic pump and for detecting the chip 102 of course of reaction.
Chip 102 is fixed on the bottom of chip carrier 101, and the roof of the first reaction film 103 contacts with the diapire of chip 102,
The roof of reactor body 104 and the diapire contact of the first reaction film 103.The main body of reactor is provided with feed pathway 110 He
Liquid outlet channel 111, feed pathway 110 and liquid outlet channel 111 all extend vertically through reactor body 104, the end of feed pathway 110
Portion for contacting with reactant liquor, the first reaction film 103 be provided with drainage lumens 109, feed pathway 110 and liquid outlet channel 111 all with
Drainage lumens 109 connects.The sidewall of chip carrier 101 and the sidewall of reactor body 104 are provided with crawl hole 107, chip carrier 101
Capture hole 107 for being connected with support member.In the preferred embodiment of the present invention, reactor also includes that second is anti-
Answer film 106, the diapire laminating of the second reaction film 106 and reactor body 104, liquid outlet channel 111 and the second reaction film 106 phase
Connect.
Peristaltic pump is connected with liquid outlet channel 111.Peristaltic pump provides the power of reactant liquor flowing, it is ensured that reactant liquor is according to rule
The path flowing drawn.
Capturing hole 107 and be used for fixing the position of this device, chip carrier 101 and reactor body 104 are provided with crawl hole
107 so that active force can be provided when fixing to keep the relative position between chip carrier 101 and reactor body 104, keep away
Exempt to loosen between chip carrier 101 and reactor body 104, and then make the path generation of reactant liquor obscure.
Embodiment 4
The present embodiment provides a kind of one-time reaction liquid to draw reactor, and it is inhaled with the one-time reaction liquid in embodiment 2
The difference taking reactor is that feeding-passage is different with the set-up mode of tapping channel, and remaining content refers to embodiment 2,
This repeats no more.
Concrete, in the present embodiment, feeding-passage includes first feeding-passage 112 and second feeding-passage 113 two parts,
Wherein, the first feeding-passage 112 is for operator's sample introduction, and the second feeding-passage 113 is vertically arranged and with tapping channel 111 also
Row is arranged.
As shown in figure 11, in the present embodiment, the second feeding-passage 113 is four passages being parallel to each other, the arrow in Figure 11
Head represents the flow direction of reactant liquor.First reactant liquor adds from the first feeding-passage 112, is being arranged at tapping channel thereafter
Under the power of 111 ends drives, the reactant liquor in the first feeding-passage 112 enters in the second feeding-passage 113, and anti-with first
Answer film 103 to react, be drawn out of thereafter by tapping channel 111.In the present embodiment, tapping channel 111 is similarly 4, and every goes out
Material passage 111 is corresponding with second feeding-passage 113.
Embodiment 5
The present embodiment provides a kind of one-time reaction liquid to draw reactor, and it is inhaled with the one-time reaction liquid in embodiment 2
The difference taking reactor is that feeding-passage 110 is different with the set-up mode of tapping channel 111, and remaining content refers to embodiment 2
, do not repeat them here.
Concrete, in the present embodiment, as shown in figure 12, in figure, the direction of arrow represents the reactant liquor flow direction in the reactor,
Wherein, the first reaction film 103 is arranged at reactor body 104 upper end, and the second reaction film is arranged at reactor body 104 lower end,
Feeding-passage 110 and tapping channel 111 are the most back and forth arranged in reactor body 104, and the reciprocal fluid passage arranged
Two ends contact with the first reaction film 103 and the second reaction film 106 respectively up and down, and such reactant liquor is at by tapping channel 111
Power drive under in fluid passage between feeding-passage 110 and tapping channel 111 the most reciprocal flowing and with the first reaction
Film 103 and the second reaction film 106 haptoreaction, can be greatly improved reactant liquor and the first reaction film 103 and the second reaction film 106
Between contact area and the response time, improve reaction effect.
Unless specifically stated otherwise, the parts, step, numerical expression and the numerical value that illustrate the most in these embodiments are also
Do not limit the scope of the invention.
The device that the embodiment of the present invention is provided, it realizes principle and the technique effect of generation and preceding method embodiment phase
With, for briefly describing, the not mentioned part of device embodiment part, refer to corresponding contents in preceding method embodiment.
In all examples being illustrated and described herein, any occurrence should be construed as merely exemplary, and not
Being as restriction, therefore, other examples of exemplary embodiment can have different values.
It should also be noted that similar label and letter represent similar terms, therefore, the most a certain Xiang Yi in following accompanying drawing
Individual accompanying drawing is defined, then need not it be defined further and explains in accompanying drawing subsequently.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for the skill of this area
For art personnel, the present invention can have various modifications and variations.All within the spirit and principles in the present invention, that is made any repaiies
Change, equivalent, improvement etc., should be included within the scope of the present invention.
Claims (10)
1. a full-automatic ProteinChip Analysis instrument, it is characterised in that include frame, shuttle, viewing system, reaction system
System, electric-control system and motor system, described viewing system, response system, motor system and electric-control system are all with described frame even
Connecing, described response system includes that reactor, described shuttle are used for accommodating sample, and described sample is for connecing with described reactor
Touching and react, described frame includes that workbench, described response system include reaction bench, described reaction bench and described workbench
Connecting, described motor system and described response system all electrically connect with described electric-control system, and described viewing system is used for observing institute
Stating the response situation of reactor, described motor system includes the mechanical arm for carrying out experimental implementation, and described mechanical arm is used for will
Described reactor transfers to described reaction bench, and described frame also includes that suction nozzle box, described suction nozzle box are used for placing disposable tip,
Described mechanical arm is additionally operable to capture described disposable tip and add described sample in described reactor.
Analyser the most according to claim 1, it is characterised in that described frame includes guide rail, and described mechanical arm is with described
Slide connects, and described electric-control system is used for controlling described mechanical arm along described slide.
Analyser the most according to claim 2, it is characterised in that described mechanical arm includes first and second, described
First is connected with described slide, and described first includes X direction guiding rail, and described X direction guiding rail is along the height side of described frame
To setting, described second is slidably connected with described X direction guiding rail, described electric-control system be additionally operable to drive described second along described
X direction guiding rail slides.
Analyser the most according to claim 1, it is characterised in that described analyser also includes rotating disk and suction nozzle box, institute
Stating suction nozzle box to be connected with described workbench or described frame, described suction nozzle box has deposited multiple disposable tip, described rotating disk
Being rotationally connected with described workbench, the top of described rotating disk is placed with at least one described shuttle and at least one reacts
Device, described electric-control system is additionally operable to drive described rotating disk to rotate, described mechanical arm be additionally operable to capture described disposable tip with
Extract described sample, and described sample is transferred to described reactor, the direction, rotary centerline place of described rotating disk and institute
The short transverse stating frame is parallel.
Analyser the most according to claim 4, it is characterised in that described workbench is additionally provided with used up for concentration
Secondary property suction nozzle abandon mouth, described frame also includes discarded collecting box, and described discarded collecting box is positioned at the bottom of described workbench,
Described disposable tip can fall into described discarded collecting box from the described mouth that abandons.
Analyser the most according to claim 1, it is characterised in that described frame also includes benchmark table top and matter storage lattice, institute
Stating matter storage lattice and be arranged on the bottom of described benchmark table top, described workbench is arranged on the top of described benchmark table top.
Analyser the most according to claim 1, it is characterised in that described reactor includes that the chip removably connected is with anti-
Device main body, the surface configuration of described chip is answered to have active organism probe, described active organism probe to be set to multiple array, each
The all corresponding protein array micro unit of individual active organism probe, described active organism probe is for combining target molecule life
Becoming bimolecular complexes, described viewing system is for observing the change procedure of active organism probe.
Analyser the most according to claim 7, it is characterised in that described analyser also includes for overturning described chip
Turning device, described turning device is connected with described reaction bench, and described turning device includes rotating movable part, described activity
The rotary centerline level of part, described chip can rotate 180 ° by described turning device.
Analyser the most according to claim 1, it is characterised in that described viewing system includes observation platform, oval thickness group
Part and ccd video camera, described observation platform is connected with described frame, described observation platform reactor after placing response, described
Oval thickness assembly is used for extending polarized lightwave and irradiating described reactor, and described ccd video camera is due in detection reflecting light
The sample message that is loaded with also stores to generate the form of digital picture.
Analyser the most according to claim 9, it is characterised in that described analyser also includes for purge reactor
The cleaning systems of surface residue, described cleaning systems electrically connect with described electric-control system, described cleaning systems include rinsing table,
Nitrogen machine, conduit, syringe pump and soda liquor container, one end of described conduit connects with described soda liquor container, described conduit
The other end points to described rinsing table, and described syringe pump is for moving the cleanout fluid in described soda liquor container along conduit and rinsing
Described rinsing table, described rinsing table is connected with described workbench, and described nitrogen machine is for drying up be positioned on described observation platform anti-
The roof answering device, described mechanical arm to be additionally operable to move to reacted reactor described rinsing table is rinsed, and will rinse
After reactor be placed in described observation platform.
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| CN201610789335.5A CN106226540B (en) | 2016-08-31 | 2016-08-31 | Full-automatic protein chip analyzer |
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| CN201610789335.5A CN106226540B (en) | 2016-08-31 | 2016-08-31 | Full-automatic protein chip analyzer |
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| CN113399304A (en) * | 2021-06-21 | 2021-09-17 | 上海金东唐科技有限公司 | Chip detection equipment |
| CN113433039A (en) * | 2021-06-25 | 2021-09-24 | 国家纳米科学中心 | Nanoparticle synthesis and detection integrated equipment and use method thereof |
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| CN106226540B (en) | 2018-09-14 |
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