CN210222038U - Metabolite detection pretreatment automation equipment - Google Patents
Metabolite detection pretreatment automation equipment Download PDFInfo
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- CN210222038U CN210222038U CN201920630185.2U CN201920630185U CN210222038U CN 210222038 U CN210222038 U CN 210222038U CN 201920630185 U CN201920630185 U CN 201920630185U CN 210222038 U CN210222038 U CN 210222038U
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Abstract
The utility model discloses an automatic device for the pretreatment of metabolite detection, which comprises a base; the device also comprises a reagent rack for placing pretreatment reagents; the oscillation incubator is used for oscillating and incubating a 96-hole reaction plate, a temperature control module and an oscillation incubation module are arranged on the oscillation incubator, and a heat cover module is arranged on the side of the oscillation incubator; a plate sealing molding machine for sealing the 96-hole reaction plate or the determination plate, wherein the plate sealing molding machine is provided with a plate sealing machine module; the liquid transferring device is used for transferring the reagent and is provided with a liquid transferring module and a liquid transferring gun; a moving rail platform for supporting a pipetting device; the host is used for controlling the oscillation incubation module, the liquid transfer module and the plate closing machine module to operate; the reagent rack is arranged in the base. The device puts into reagent frame to interior label reagent and extraction reagent, and the 96 hole reaction plates and the survey board of making the mark are placed and can full automatic operation on liquid-transfering device, and whole unmanned on duty has reduced the contaminated probability of waiting to examine the thing, has reduced operating personnel's intensity of labour simultaneously.
Description
Technical Field
The utility model relates to a metabolite detects technical field, specifically is a metabolite detects pretreatment automation equipment.
Background
The mass spectrometry detection technology is a novel detection technology which is developed in the last decade, and the mass spectrometer which is the mainstream in the market is mainly provided by Waters, ABsciex, Agilent and the like, and any mass spectrometer firstly requires to extract metabolites from a biological sample before detection, and then introduces the metabolites and internal standard substances in an extraction reagent into the mass spectrometer through a sample introduction device for detection.
The conventional mass spectrometry detection pretreatment comprises the steps of preparing an extraction working solution containing an internal standard, extracting a metabolite to be detected, transferring the metabolite solution to be detected, sealing a detection plate and the like.
Preparing an extraction working solution containing an internal standard: the internal standard is an important internal standard substance for completing mass spectrum detection, the internal standard substances are usually prepared respectively according to attributes and need to be combined together during experimental treatment, and because the concentrated internal standard solution has few samples, the manual preparation process is complex, the internal standard solution is easy to overturn or sample adding errors, the probability of experimental failure can be increased, and the process generally needs about 30 minutes.
Extraction of metabolites to be detected: adding the prepared internal standard working solution into 96-hole micropores pre-punched with 3mm blood spots according to 100 microliters/hole, sealing the 96-hole reaction plate, wherein the sealing aims at preventing the volatilization of the extraction solution, the mutual interference among different micropores and the possible pollution, placing the 96-hole reaction plate on an oscillation incubator for oscillation incubation, and carrying out oscillation incubation at the temperature of 45 ℃ for 30-60 minutes to complete the extraction of the metabolites to be detected, wherein the process takes about 60-90 minutes.
Transfer of the metabolite solution to be tested: taking a 96-well plate for determination, marking, corresponding to the extraction plate, and transferring the upper-layer extraction solution in the extraction micro-well plate to the 96-well plate for determination according to the one-to-one correspondence of the positions, wherein the process takes about 10 minutes.
Sealing of the assay plate: the purpose of using the aluminium foil shrouding, shrouding is in order to prevent that the extraction solution from volatilizing, and this process needs about 2 minutes, and the membrane that the position corresponds to be covered in the puncture of above-mentioned shrouding advances a sample can not have the glue material.
To the complicated experimental steps, some instruments replace manual operation in the market, and as the mass spectrometry technology is a brand new technology in the market at present, single equipment for each step is provided, for example, pipetting can be performed by various pipetting workstations, shaking incubation can be completed by a shaking incubator, and sealing of a plate can be completed manually or by a plate closing instrument. The pipetting station can dispense liquid or transfer in an 8/12/96 well format. The operator needs to put the interior label working solution into the liquid transfer groove after diluting and preparing, manually transfer liquid or set up the procedure and move liquid, use in order to wait for the follow-up experiment after marking the board. Metabolite pretreatment, the extraction working solution that contains the interior label is prepared, treat the metabolite extraction, the transfer that treats the analyte solution can be realized by moving liquid workstation, but the sealed needs of 96 orifice plates seal the membrane, work cooperation such as dyestripping, a plurality of steps need artifical mark, although some work is accomplished by the workstation, whole still needs artifical intervention, can't realize whole process unmanned on duty, in this process, can increase and treat the contaminated probability of analyte, and increased operating personnel's intensity of labour.
Disclosure of Invention
In order to overcome the defect among the above-mentioned prior art, the utility model provides a metabolite detects preceding automation equipment, this automation equipment degree of automation is high, can realize the full-automatic of metabolite detection preceding processing, prepare including the extraction working solution that contains interior label, treat the extraction of metabolite (including the extraction working solution distribution that contains interior label, the reaction plate is sealed, the reaction plate oscillation is hatched), treat the transfer of metabolite solution, survey the sealed of board, the end product can directly be used for detecting, whole unmanned on duty, the contaminated probability of waiting to examine the thing has been reduced, operating personnel's intensity of labour has been reduced simultaneously, be favorable to this automation equipment in popularization and application in this field.
In order to realize the aim, the utility model adopts the following technical proposal that the automatic device for the metabolite detection pretreatment comprises a base; the device also comprises a reagent rack for placing pretreatment reagents; the oscillation incubator is used for oscillating and incubating a 96-hole reaction plate, a temperature control module and an oscillation incubation module are arranged on the oscillation incubator, and a heat cover module is arranged on the side of the oscillation incubator; a plate sealing molding machine for sealing a 96-well reaction plate or a determination plate, the plate sealing molding machine being provided with a plate sealing machine module; a pipetting device for transferring reagents, said pipetting device being provided with a pipetting module and a pipetting gun; a moving rail platform for supporting the pipetting device; the host is used for controlling the oscillation incubation module, the liquid transfer module and the plate closing machine module to operate; the reagent is arranged in the base.
As an optimized proposal of the utility model, the lower end of the reagent rack is provided with a reagent rack base for placing the reagent rack.
As a preferred scheme of the utility model, reagent frame is equipped with the reagent groove.
As an optimized proposal of the utility model, the reagent grooves are a plurality of and are distributed at equal intervals.
As an optimized proposal of the utility model, the base is provided with a gun head storage tank for placing the liquid-transfering gun.
As a preferred proposal of the utility model, the base is provided with a reaction plate storage position for placing a 96-hole reaction plate.
As an optimized scheme of the utility model, the base is equipped with the incubator installation position that is used for placing the vibration incubator.
As an optimized proposal of the utility model, the base is provided with a storage position for placing the hot cover module.
As a preferred embodiment of the present invention, the incubator mounting position is lower than the surface of the base.
As an optimized proposal of the utility model, the base is provided with a closed cover outside.
As an optimized proposal of the utility model, the gun head of the liquid-transfering gun is made of plastic material.
The utility model has the advantages of it is following: the utility model provides a metabolite detects preceding processing automation equipment, full-automatic metabolite detects the full-automatic preceding processing that has realized, prepare including the extraction working solution that contains the interior label, treat the metabolite extraction, treat the transfer of detection metabolite solution, the steps such as sealed of survey board, full-automatic unmanned on duty, whole process manual operation time is short, put into interior label reagent and extraction reagent and reagent frame, 96 hole reaction plates and survey board of making the mark place can full-automatic operation on moving the liquid device, whole unmanned on duty, the contaminated probability of waiting to examine the thing has been reduced, operating personnel's intensity of labour has been reduced simultaneously, be favorable to this automation equipment in popularization and application in this field.
Drawings
FIG. 1 is a schematic structural view of an automated apparatus for pretreatment of metabolite detection according to an embodiment of the present invention;
FIG. 2 is a schematic illustration of the position of the pipette.
Reference numerals: 1. a gun head storage tank; 2. a reagent tank; 3. a reaction plate storage position; 4. an incubator mounting site; 5. closing a plate die machine; 6. a liquid transferring gun; 7. moving the rail platform; 8. a base.
Detailed Description
The following describes embodiments of the present invention in detail with reference to the accompanying drawings.
Example (b): as shown in fig. 1 to 2, an automatic metabolite detection pretreatment device includes a base 8, and further includes a reagent rack for holding pretreatment reagents; the oscillation incubator is used for oscillating and incubating a 96-hole reaction plate, a temperature control module and an oscillation incubation module are arranged on the oscillation incubator, and a heat cover module is arranged on the side of the oscillation incubator; a plate sealing molding machine 5 for sealing the 96-well reaction plate or the assay plate, said plate sealing molding machine 5 being provided with a plate sealing machine module; a pipetting device for transferring reagents, said pipetting device being provided with a pipetting module and a pipetting gun 6; a moving guide platform 7 for supporting the pipetting device; the host is used for controlling the oscillation incubation module, the liquid transfer module and the plate closing machine module to operate; the reagent is mounted in the base 8.
In this embodiment, the internal standard reagent and the extraction reagent are placed in the reagent tank 2 on the reagent rack, and the 96-well reaction plate and the assay plate which are marked are placed on the liquid transfer device to run fully automatically, so that the whole process is unattended, the probability of pollution of the object to be detected is reduced, the labor intensity of operators is reduced, and the popularization and the application of the automatic device in the field are facilitated.
With reagent frame, vibration incubator, shrouding mould machine 5, move the liquid device and all set up on base 8 to through the lug connection between the different experimental steps that the removal guide rail platform 7 realized, reduced the sample mark work that a plurality of links need, accelerated whole test speed greatly.
The automation device is provided with modules for mixing, pipetting, temperature control, sealing and the like, so the device can be developed again according to actual needs, other operation steps are added, the expansibility is large, the 96-hole reaction plate is used as a minimum operation unit for operation, but the amplification can be realized by loading the oscillation incubator module, and the high-throughput operation can be flexibly realized.
Still can set up the mixing module on the reagent frame, then need not manual operation and can realize treating the mixing of detecting liquid, the later stage of being convenient for is treated and is detected detecting liquid, and automatic mixing is more effectual than artifical mixing, and the error of production is low.
In order to ensure the stability of the liquid to be detected in the moving process and reduce the probability of liquid sprinkling, liquid leakage and the like, a reagent rack base used for placing a reagent rack is arranged at the lower end of the reagent rack.
Reagent frame still is equipped with reagent groove 2, sets up a plurality of reagent grooves 2 as required, and the equidistance distributes between reagent groove 2 and the reagent groove 2, avoids reagent and reagent each other because the contact causes reagent alternately, and then reduces the testing result.
The base 8 is equipped with the rifle head hold up tank 1 that is used for placing above-mentioned liquid-transfering gun 6, avoids liquid-transfering gun 6 to contact other equipment, guarantees the cleanliness of the rifle head of liquid-transfering gun 6.
The base 8 is equipped with the reaction plate that is used for placing 96 hole reaction plates and deposits position 3, and the reaction plate is deposited position 3 and is deposited the looks adaptation of 96 hole reaction plates, and caves in 8 upper surfaces of base, guarantees 96 hole reaction plates at the stability of removal in-process, avoids its aversion.
The base 8 is equipped with the incubator installation position 4 that is used for placing the oscillation incubator, and incubator installation position 4 is less than base 8 upper surface, guarantees the stability of oscillation incubator in the use.
The electric auxiliary heating module is additionally arranged on the oscillation incubator, so that the temperature on the oscillation incubator can be guaranteed within a reasonable range, the probability of condensation caused by temperature difference after the oscillation incubation is finished is further reduced, and the safe transfer of the detected metabolites is guaranteed.
The sealing cover is arranged outside the base 8, so that the automatic device can operate in a closed system, the influence of organic solution volatilization on operators is avoided, and the mutual pollution among samples is avoided.
The liquid-transfering gun 6 is made of plastic material, so that the weight is light and the taking is convenient.
The whole automatic device for metabolite detection pretreatment is operated at room temperature.
The detection method of the automatic device for the metabolite detection pretreatment comprises the following steps:
the device is used for carrying out experimental treatment operation on 1000 newborn blood spot samples, parallel control is carried out with manual operation experimental treatment, detection and analysis are respectively completed on the same equipment, data results are analyzed through a spread software, no significant difference exists between every two data results for clinical analysis (P is less than 0.05), and precision indexes are obviously superior to manual experimental operation (8% VS 10%) by adopting the device.
Reagent materials: a non-derivatization multi-amino acid, carnitine and succinylacetone determination kit;
sample experimental treatment
1. The newborn blood spot specimens are sequentially placed into a 96-well reaction plate (provided by a kit) in a 3.2mm size according to the experimental requirement, marked (optional bar codes) are made, and the 96-well assay plate is taken and marked (optional bar codes) are made.
2. Respectively placing the amino acid internal standard solution, the carnitine internal standard solution and the succinylacetone treatment solution on corresponding positions of a reagent tank, adding an extraction solution with a proper volume to a corresponding scale line of the reagent tank, and respectively placing a reaction plate loaded with newborn blood spots and a corresponding determination plate on a loading frame. (the internal standard solution, the extraction solution, the reaction plate and the measurement plate are provided by a kit)
And the computer work site hit running program automatically runs the program.
The metabolite detects preceding automation equipment in this embodiment, full-automatic metabolite detects preceding full-automatic realization, including the extraction working solution preparation that contains the interior label, treat the metabolite extraction, treat the transfer of metabolite solution, the steps such as sealed of survey board, full-automatic unmanned on duty, whole process manual operation time is short, put interior label reagent and extraction reagent into the reagent frame, 96 hole reaction plates and survey boards of well-done mark place can full-automatic operation on the liquid-transfering device, whole unmanned on duty, the contaminated probability of the metabolite of waiting to detect has been reduced, operating personnel's intensity of labour has been reduced simultaneously, be favorable to this automation equipment popularization and application in this field.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention; thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Although reference numeral 1, the lance tip storage tank, is used more herein; 2. a reagent tank; 3. a reaction plate storage position; 4. an incubator mounting site; 5. closing a plate die machine; 6. a liquid transferring gun; 7. moving the rail platform; 8. base, etc., but does not exclude the possibility of using other terms. These terms are used merely to more conveniently describe and explain the nature of the present invention; they are to be construed in a manner that is inconsistent with the spirit of the invention.
Claims (10)
1. An automatic device for metabolite detection pretreatment comprises a base (8); the method is characterized in that: the device also comprises a reagent rack for placing pretreatment reagents; the oscillation incubator is used for oscillating and incubating a 96-hole reaction plate, a temperature control module and an oscillation incubation module are arranged on the oscillation incubator, and a heat cover module is arranged on the side of the oscillation incubator; a plate sealing molding machine (5) for sealing a 96-well reaction plate or a measurement plate, wherein the plate sealing molding machine (5) is provided with a plate sealing machine module; a pipetting device for transferring reagents, said pipetting device being provided with a pipetting module and a pipetting gun (6); a moving guide platform (7) for supporting the pipetting device; the host is used for controlling the oscillation incubation module, the liquid transfer module and the plate closing machine module to operate; the reagent is arranged in the base (8).
2. The automatic metabolite detection pretreatment device according to claim 1, wherein: the lower end of the reagent rack is provided with a reagent rack base for placing the reagent rack.
3. The automatic metabolite detection pretreatment device according to claim 2, wherein: the reagent shelf is provided with a reagent groove (2).
4. The automatic metabolite detection pretreatment device according to claim 3, wherein: the reagent grooves (2) are distributed at equal intervals.
5. The automatic metabolite detection pretreatment device according to claim 1, wherein: the base (8) is provided with a gun head storage groove (1) for placing the pipette gun (6).
6. The automatic metabolite detection pretreatment device according to claim 1, wherein: the base (8) is provided with a reaction plate storage position (3) for placing a 96-hole reaction plate.
7. The automatic metabolite detection pretreatment device according to claim 1, wherein: the base (8) is provided with an incubator mounting position (4) for placing an oscillation incubator.
8. The automatic metabolite detection pretreatment device according to claim 1, wherein: the base (8) is provided with a storage position for placing the hot cover module.
9. The automatic metabolite detection pretreatment device according to claim 7, wherein: the incubator mounting position (4) is lower than the surface of the base (8).
10. The automatic metabolite detection pretreatment device according to claim 1, wherein: and a closed cover is arranged outside the base (8).
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| Application Number | Priority Date | Filing Date | Title |
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| CN201920630185.2U CN210222038U (en) | 2019-05-05 | 2019-05-05 | Metabolite detection pretreatment automation equipment |
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| CN201920630185.2U CN210222038U (en) | 2019-05-05 | 2019-05-05 | Metabolite detection pretreatment automation equipment |
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| CN210222038U true CN210222038U (en) | 2020-03-31 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111493892A (en) * | 2020-04-22 | 2020-08-07 | 睿科生化科技(广州)有限公司 | Automatic input system and method for blood sampling card samples |
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2019
- 2019-05-05 CN CN201920630185.2U patent/CN210222038U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111493892A (en) * | 2020-04-22 | 2020-08-07 | 睿科生化科技(广州)有限公司 | Automatic input system and method for blood sampling card samples |
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