CN218637406U - Multi-sample extraction device for nucleic acid extractor - Google Patents

Abstract

The utility model discloses a multi-sample extraction device for a nucleic acid extractor, which comprises a shell, a multi-channel manual pipettor, a lifting driving mechanism, a lifting plate, a clamping seat, a pressing mechanism, an electric sliding table and a transverse moving seat; the pressing mechanism comprises a linear guide rail, a mounting plate, a second linear driver, a push-pull type electromagnet and a pressing plate. The loading of waiting to examine the sample's sample pipe support through electronic slip table drive, the sample pipe support of loading the centrifuging tube, the suction head loading frame of loading the suction head, the interpolation reagent container of loading proteinase K reagent, the interpolation reagent container of loading buffer solution R1, the suction head abandons the box and moves to the multichannel manual pipettor directly under in proper order, descend through the manual pipettor of first sharp driver drive multichannel, press control button or unload the button through pressing means, realize accurate imbibition, tapping, unload the function of suction head and installation suction head, reduce the error probability of artificial operation, and the work efficiency is improved.

Description

Multi-sample extraction device for nucleic acid extractor

Technical Field

The utility model relates to a nucleic acid extraction technical field, especially a many samples extraction element are used to nucleic acid extraction appearance.

Background

A Nucleic Acid Extraction System (Nucleic Acid Extraction System) is an instrument which automatically finishes the Extraction of sample Nucleic Acid by using matched Nucleic Acid Extraction reagents. The method is widely applied to various fields of disease control center, clinical disease diagnosis, blood transfusion safety, forensic medicine identification, environmental microorganism detection, food safety detection, animal husbandry, molecular biology research and the like.

The centrifugal column method nucleic acid extractor mainly adopts a method of combining a centrifugal machine and an automatic pipetting device, and the flux is generally 1-12 samples. The conventional automatic liquid-transfering device has the advantages of almost the same operation time for extracting a sample as that of manual extraction, incapability of improving the actual working efficiency, high price, large occupied space and suitability for large laboratories with sufficient expenditure. Therefore, it is necessary to develop an economical and small-sized nucleic acid sample extraction device to solve this problem.

The above description is included in the technical knowledge of the inventors, and does not necessarily constitute a prior art.

SUMMERY OF THE UTILITY MODEL

In view of the above, it is necessary to provide a multi-sample extraction apparatus for a nucleic acid extractor.

In order to achieve the above object, the utility model provides a many samples extraction device are used to nucleic acid extraction appearance, include:

a housing;

the top of the multi-channel manual liquid transfer device is provided with a control button for controlling liquid suction and liquid discharge and a discharging button for discharging a suction head;

a lifting driving mechanism installed in the casing;

the lifting plate is connected with the lifting driving mechanism and driven to lift by the lifting driving mechanism;

the clamping seat is fixedly arranged on the lifting plate and is used for clamping with the multi-channel manual pipettor;

a pressing mechanism mounted on the lifting plate for selectively pressing the control button or the unloading button;

the electric sliding table is arranged on the inner wall of the bottom end of the machine shell;

the transverse moving seat is arranged on a sliding seat of the electric sliding table, a plurality of grooves are formed in the top of the transverse moving seat along the length direction of the electric sliding table, and all the grooves can be moved to the position under the clamping seat under the driving of the electric sliding table.

Preferably, the lifting driving mechanism comprises a first linear driver, a guide rod and a guide sleeve, the first linear driver and the guide sleeve are fixedly connected with the casing, the output end of the first linear driver is connected with the lifting plate, the guide rod is slidably mounted in the guide sleeve, and one end of the guide rod is fixedly connected with the lifting plate.

Preferably, the pressing mechanism comprises a linear guide rail, a mounting plate, a second linear driver and a push-pull electromagnet, the linear guide rail and the push-pull electromagnet are fixedly mounted on the lifting plate, the mounting plate is fixedly mounted on a sliding block of the linear guide rail, the second linear driver is fixedly mounted on the mounting plate, and the output end of the push-pull electromagnet is fixedly connected with the mounting plate.

Preferably, the output end of the second linear actuator is provided with a pressing plate for pressing the control button and the unloading button.

Preferably, a fastening handle is installed on the clamping seat in a threaded mode.

Preferably, a sample tube rack, a container for adding reagents, a suction head loading frame and a suction head discarding box are respectively arranged in the grooves.

Preferably, the front end of the casing is provided with an operation opening.

The beneficial effects of the technical scheme are as follows: the device selects a multi-channel manual pipettor for pipetting, a sample pipe frame loaded with a sample to be inspected, a sample pipe frame loaded with a centrifugal pipe, a suction head loading frame loaded with a suction head, a pipe frame loaded with a proteinase K reagent, an additive reagent container loaded with a buffer solution R1 and a suction head discarding box are sequentially moved to the position under the multi-channel manual pipettor by driving the multi-channel manual pipettor to descend by a first linear driver, and a control button or a discharging button is pressed by a pressing mechanism, so that the functions of accurately sucking liquid, discharging the suction head and mounting the suction head are realized, the error probability of manual operation is reduced, and the working efficiency is improved; and the device has small volume and is suitable for small laboratories.

Drawings

Fig. 1 is a schematic structural diagram of an embodiment of the present invention;

fig. 2 is a front view of an embodiment of the present invention;

fig. 3 is a schematic view of another state of the present invention;

in the figure, 1, a housing; 2. a multi-channel manual pipettor; 3. a control button; 4. a suction head; 5. a release button; 6. a lifting plate; 7. a card holder; 8. an electric sliding table; 9. a traversing seat; 10. a groove; 11. a first linear driver; 12. a guide bar; 13. a guide sleeve; 14. a linear guide rail; 15. mounting a plate; 16. a second linear actuator; 17. a push-pull electromagnet; 18. a pressing plate; 19. tightening the handle; 20. a sample pipe frame; 21. a container for adding a reagent; 22. a suction head loading frame; 23. a suction head discarding box; 24. and an operation port.

Detailed Description

In order to make the above objects, features and advantages of the present invention more comprehensible, embodiments of the present invention are described in detail below with reference to the accompanying drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein, as those skilled in the art will be able to make similar modifications without departing from the spirit and scope of the present invention.

Referring to fig. 1 to 3, an embodiment of the present application provides a multi-sample extraction device for a nucleic acid extractor, including:

a housing 1; an operation port 24 is arranged at the front end of the casing 1, and the internal condition of the casing can be observed conveniently through the operation port 24, so that the following multi-channel manual liquid transfer device 2 can be installed conveniently;

a multi-channel manual liquid shifter 2, the top of which is provided with a control button 3 for controlling liquid absorption and liquid discharge and a discharging button 5 for discharging a suction head 4; the multichannel manual pipettor 2 can be a Dalong MicroPette manual 12-channel adjustable pipettor, so that pipetting is facilitated;

a lifting driving mechanism installed in the cabinet 1; the lifting driving mechanism comprises a first linear driver 11, a guide rod 12 and a guide sleeve 13, the first linear driver 11 and the guide sleeve 13 are fixedly connected with the machine shell 1, the output end of the first linear driver 11 is connected with the lifting plate 6, the guide rod 12 is slidably arranged in the guide sleeve 13, and one end of the guide rod 12 is fixedly connected with the lifting plate 6;

the lifting plate 6 is connected with the lifting driving mechanism and driven to lift by the lifting driving mechanism;

the clamping seat 7 is fixedly arranged on the lifting plate 6 and is used for being clamped with the multi-channel manual liquid transfer device 2; in order to further fix the multi-channel manual pipettor 2, a fastening handle 19 is installed on the clamping seat 7 in a threaded manner, and after the multi-channel manual pipettor 2 is clamped in the clamping seat 7, the multi-channel manual pipettor 2 can be tightly pressed and fixed in the clamping seat 7 through the fastening handle 19;

a pressing mechanism mounted on the lifting plate 6 for selectively pressing the control button 3 or the unloading button 5; the pressing mechanism comprises a linear guide rail 14, a mounting plate 15, a second linear driver 16 and a push-pull electromagnet 17, the linear guide rail 14 and the push-pull electromagnet 17 are both fixedly mounted on the lifting plate 6, the mounting plate 15 is fixedly mounted on a sliding block of the linear guide rail 14, the second linear driver 16 is fixedly mounted on the mounting plate 15, the output end of the push-pull electromagnet 17 is fixedly connected with the mounting plate 15, and the output end of the second linear driver 16 is provided with a pressing plate 18 for pressing the control button 3 and the unloading button 5;

the electric sliding table 8 is arranged on the inner wall of the bottom end of the machine shell 1;

and the transverse moving seat 9 is installed on a sliding seat of the electric sliding table 8, as shown in fig. 3, a plurality of grooves 10 are formed in the top of the transverse moving seat along the length direction of the electric sliding table 8, and each groove 10 can be moved to the position under the clamping seat 7 under the driving of the electric sliding table 8. The sample tube rack 20, the addition reagent container 21, the tip loading rack 22, and the tip discard cassette 23 are placed in the recess 10, respectively. Two sample tube racks 20 are provided, one of which is loaded with a sample to be tested, and the other is loaded with a centrifuge tube rack; the tip loading rack 22 is loaded with tips 4; when the lysis mixture is prepared, the required additional reagents include proteinase K reagent and buffer solution R1, and then the container loaded with the proteinase K reagent and the container loaded with the buffer solution R1 are respectively placed in the designated groove 10 in sequence.

The preparation of the lysis mixture is the first step of nucleic acid extraction, and the working steps in the preparation of the lysis mixture in this example are as follows:

s1, clamping a multi-channel manual pipettor 2 in a clamping seat 7, and tightly pressing and fixing the multi-channel manual pipettor 2 in the clamping seat 7 through a fastening handle 19;

s2, respectively placing a sample tube rack 20 loaded with a sample to be detected, the sample tube rack 20 loaded with a centrifugal tube, a suction head loading rack 22 loaded with a suction head 4, an additive reagent container 21 loaded with a proteinase K reagent, the additive reagent container 21 loaded with a buffer solution R1 and a suction head discarding box 23 in sequence into a designated groove 10;

s3, driving the transverse moving seat 9 to transversely move by the electric sliding table 8, enabling the suction head loading frame 22 to move to the position right below the suction head 4 mounting end of the multi-channel manual liquid transfer machine 2, driving the lifting plate 6 to descend by the first linear driver 11, driving the multi-channel manual liquid transfer machine 2 to descend by the lifting plate 6, enabling the suction head 4 to be clamped into the suction head 4 mounting end of the manual liquid transfer machine, and mounting the suction head 4;

s4, the lifting plate 6 is driven to ascend by the first linear driver 11, the electric sliding table 8 drives the transverse moving seat 9 to transversely move, so that the sample pipe frame 20 loaded with a sample to be detected moves to the position right below the suction head 4 of the multichannel manual pipettor 2, the pressing plate 18 is driven to descend by the second linear driver 16, the control button 3 is pressed by the pressing plate 18 to a lower stop point, and the lifting plate 6 and the multichannel manual pipettor 2 are driven to descend by the first linear driver 11 so that the suction head 4 extends into the sample pipe; the second linear driver 16 drives the pressing plate 18 to ascend, the control button 3 automatically ascends and resets, and the suction head 4 sucks a sample;

s5, driving the lifting plate 6 and the multi-channel manual pipettor 2 to ascend by the first linear driver 11, driving the transverse moving seat 9 to transversely move by the electric sliding table 8, so that the sample tube rack 20 loaded with the centrifugal tube moves to be right below a suction head 4 of the multi-channel manual pipettor 2, and driving the lifting plate 6 and the multi-channel manual pipettor 2 to descend by the first linear driver 11 so that the suction head 4 extends into the centrifugal tube; the second linear driver 16 drives the pressing plate 18 to descend, the pressing plate 18 presses the control button 3 to a lower stop point, and the sample is discharged into the centrifuge tube;

s6, the first linear driver 11 drives the lifting plate 6 and the multi-channel manual liquid transfer device 2 to ascend, the electric sliding table 8 drives the transverse moving seat 9 to transversely move, the suction head discarding box 23 is made to move right below a suction head 4 of the multi-channel manual liquid transfer device 2, the push-pull type electromagnet 17 is electrified, the telescopic end extends out of the push mounting plate 15 to transversely move, the second linear driver 16 is made to move right above the unloading button 5, the second linear driver 16 drives the pressing plate 18 to descend, the pressing plate 18 presses and unloads the suction head 4, and the suction head 4 is automatically unloaded and falls into the suction head discarding box 23;

s7, driving the transverse moving seat 9 to transversely move by the electric sliding table 8, so that the suction head loading frame 22 loaded with the suction heads 4 moves to the position right below the suction heads 4 of the multi-channel manual liquid moving device 2, and automatically installing the suction heads 4 in the same way as the step S3;

s8, the electric sliding table 8 drives the additive reagent container 21 loaded with the proteinase K reagent to move under the suction head 4 of the multi-channel manual liquid transfer device 2, and the suction head 4 sucks the proteinase K reagent according to the same principle as the S4;

s9, driving the lifting plate 6 to ascend by the first linear driver 11, driving the transverse moving seat 9 to transversely move by the electric sliding table 8, moving the sample pipe frame 20 loaded with the sample to be detected to be right below the suction head 4 of the multi-channel manual liquid shifter 2, and discharging a proteinase K reagent into a centrifugal pipe in the same principle as the S5;

s10, the first linear driver 11 drives the lifting plate 6 and the multi-channel manual liquid transfer device 2 to ascend, the electric sliding table 8 drives the transverse moving seat 9 to transversely move, so that the suction head discarding box 23 moves right below the suction head 4 of the multi-channel manual liquid transfer device 2, the suction head 4 is automatically unloaded and falls into the suction head discarding box 23 in the same principle as the S6;

s11, driving the transverse moving seat 9 to transversely move by the electric sliding table 8, so that the suction head loading frame 22 loaded with the suction heads 4 moves to the position right below the suction heads 4 of the multi-channel manual liquid transfer device 2, and automatically installing the suction heads 4 in the same way as the step S3;

s12, the electric sliding table 8 drives the additive reagent container 21 loaded with the buffer solution R1 to move under the suction head 4 of the multi-channel manual pipette 2, and the suction head 4 sucks the buffer solution R1 according to the same principle as the S4;

s13, driving the lifting plate 6 to ascend by the first linear driver 11, driving the transverse moving seat 9 to transversely move by the electric sliding table 8, moving the sample pipe frame 20 loaded with the sample to be detected to be right below the suction head 4 of the multi-channel manual liquid shifter 2, and discharging the buffer solution R1 into a centrifuge tube in the same principle as the S5;

and S14, taking out the centrifuge tube prepared in the S13, oscillating, incubating in a greenhouse, and preparing a sample lysis mixed solution.

After the sample cracking mixed liquid is prepared, the subsequent nucleic acid extraction operation can be carried out.

The technical features of the above embodiments can be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the above embodiments are not described, but should be considered as the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above embodiments only represent several embodiments of the present invention, and the description thereof is specific and detailed, but not to be construed as limiting the scope of the present invention. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several variations and modifications can be made, which are within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", and the like, indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless explicitly defined otherwise.

In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

Claims (7)

1. A multi-sample extraction device for a nucleic acid extractor, comprising:

a housing (1);

the top of the multi-channel manual liquid shifter (2) is provided with a control button (3) for controlling liquid suction and liquid discharge and a discharging button (5) for discharging a suction head (4);

it is characterized by also comprising:

a lifting drive mechanism installed in the casing (1);

a lifting plate (6) which is connected with the lifting driving mechanism and is driven by the lifting driving mechanism to lift;

the clamping seat (7) is fixedly arranged on the lifting plate (6) and is used for clamping with the multi-channel manual liquid transfer device (2);

a pressing mechanism mounted on the lifting plate (6) for selectively pressing the control button (3) or the unloading button (5);

the electric sliding table (8) is arranged on the inner wall of the bottom end of the machine shell (1);

the transverse moving seat (9) is arranged on a sliding seat of the electric sliding table (8), a plurality of grooves (10) are formed in the top of the transverse moving seat along the length direction of the electric sliding table (8), and all the grooves (10) can be moved to the position under the clamping seat (7) under the driving of the electric sliding table (8).

2. The multi-sample extraction device for the nucleic acid extractor according to claim 1, wherein the lifting drive mechanism comprises a first linear driver (11), a guide rod (12) and a guide sleeve (13), the first linear driver (11) and the guide sleeve (13) are fixedly connected with the housing (1), the output end of the first linear driver (11) is connected with the lifting plate (6), the guide rod (12) is slidably mounted in the guide sleeve (13), and one end of the guide rod (12) is fixedly connected with the lifting plate (6).

3. The multi-sample extraction device for the nucleic acid extractor according to claim 1, wherein the pressing mechanism comprises a linear guide (14), a mounting plate (15), a second linear actuator (16), and a push-pull electromagnet (17), the linear guide (14) and the push-pull electromagnet (17) are both fixedly mounted on the lifting plate (6), the mounting plate (15) is fixedly mounted on a slide block of the linear guide (14), the second linear actuator (16) is fixedly mounted on the mounting plate (15), and an output end of the push-pull electromagnet (17) is fixedly connected with the mounting plate (15).

4. The multiple-sample isolation apparatus for nucleic acid isolation instrument according to claim 3, wherein a pressing plate (18) for pressing the control button (3) and the removal button (5) is attached to the output end of the second linear actuator (16).

5. The multi-sample extraction device for the nucleic acid extractor as claimed in claim 1, wherein the cartridge (7) is threadedly mounted with a fastening handle (19).

6. The multiple-specimen collecting device for nucleic acid isolation instrument according to claim 1, wherein a sample tube holder (20), an additive reagent container (21), a tip loading holder (22), and a tip discarding cassette (23) are respectively placed in said recess (10).

7. The multi-sample extraction device for a nucleic acid isolation instrument according to claim 1, wherein an operation port (24) is provided at a front end of the housing (1).

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