CN219260002U - Magnetic bead adsorption mechanism for DNA plasmid purification - Google Patents
Magnetic bead adsorption mechanism for DNA plasmid purification Download PDFInfo
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- CN219260002U CN219260002U CN202223110041.5U CN202223110041U CN219260002U CN 219260002 U CN219260002 U CN 219260002U CN 202223110041 U CN202223110041 U CN 202223110041U CN 219260002 U CN219260002 U CN 219260002U
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Abstract
The utility model relates to the technical field of DNA plasmid magnetic bead purification and extraction, in particular to a magnetic bead adsorption mechanism for DNA plasmid purification, which comprises magnetic rods and rod sleeves which are arranged in a one-to-one correspondence manner, wherein the magnetic rods and the rod sleeves are respectively lifted and lowered along the vertical direction through lifting driving components, and the magnetic rods conduct magnetic bead adsorption work through the rod sleeves; the bottom of the lifting driving component is provided with a horizontal driving component which drives the magnetic rod and the rod sleeve to synchronously perform horizontal movement switching, the lifting driving component is utilized to drive the magnetic rod and the rod sleeve to perform lifting movement respectively, so that the magnetic rod and the rod sleeve are independent in lifting adjustment, and meanwhile, the magnetic rod and the rod sleeve can be smoothly inserted and separated, so that the magnetic beads can be adsorbed, and the magnetic beads can be separated rapidly.
Description
Technical Field
The utility model relates to the technical field of DNA plasmid magnetic bead purification and extraction, in particular to a magnetic bead adsorption mechanism for DNA plasmid purification.
Background
The magnetic bead is characterized in that the surface of the magnetic bead micro-bead is coated with a layer of material capable of undergoing centrifugal exchange, such as DEAE, COOH and the like, so as to achieve the aim of adsorbing nucleic acid. The purification principle corresponding to magnetic bead microbeads with different properties is inconsistent. The great advantage of using the magnetic bead method for purifying nucleic acids is automation. The magnetic beads can be gathered or dispersed under the magnetic field condition, so that the manual operation procedures required by centrifugation and the like can be thoroughly eliminated.
The only magnetic bead purification equipment on the market at present is a Kingfisher magnetic bead protein automatic purification instrument which is proposed by Thermo Scientific company, and the magnetic bead method is utilized to purify the protein by inserting a magnetic rod into a container. However, the maximum sample volume capable of being purified is only 5ml, the liquid transfer treatment needs to be manually participated, and the pollution cannot be controlled by adopting an open working station, so that the requirements of customers on realizing sample treatment of tens of milliliters and one hundred milliliters automatically cannot be met.
The Chinese patent with the patent application number of 201811009399.4 discloses a full-automatic immunomagnetic bead purification instrument, which comprises a complete machine shell, a start-stop button, a touch screen, a base, an electric box arranged on the base, a magnetic rod assembly, a magnetic rod sleeve assembly, a test tube strip placing assembly, an integral lifting assembly, an integral translation assembly and a sleeve lifting assembly, and high-efficiency purification and enrichment of samples are realized through interaction among different structures.
Although the above-mentioned patent documents disclose a combination structure of the magnetic rod and the magnetic rod sleeve, the solution described in the above-mentioned patent documents still has a problem that the lifting adjustment of the magnetic rod and the rod sleeve is not independent.
Disclosure of Invention
According to the magnetic bead adsorption mechanism for purifying the DNA plasmids, the lifting driving assembly is utilized to drive the magnetic rod and the rod sleeve to lift and move respectively, so that the lifting adjustment of the magnetic rod and the rod sleeve is independent, and meanwhile, the magnetic rod and the rod sleeve can be inserted and separated smoothly, so that the magnetic beads can be adsorbed and can be separated rapidly.
In order to achieve the above purpose, the present utility model provides the following technical solutions:
a magnetic bead adsorption mechanism for DNA plasmid purification, comprising:
the magnetic rods and the rod sleeves are arranged in a one-to-one correspondence manner, the magnetic rods and the rod sleeves are respectively lifted and arranged along the vertical direction through a lifting driving assembly, and the magnetic rods conduct the adsorption work of the magnetic beads through the rod sleeves;
the bottom of the lifting driving component is provided with a horizontal driving component for driving the magnetic rod and the rod sleeve to synchronously perform horizontal movement switching.
As an improvement, the magnetic rod comprises a rod body and a magnetic suction head;
the rod body is connected with the lifting driving assembly through a mounting seat arranged at the top;
the magnetic suction head is positioned at the bottom of the rod body and has magnetic force.
As an improvement, a plurality of rod sleeves which are arranged in a straight line are integrally arranged, and each rod sleeve comprises a sleeve body and a connecting sheet.
As an improvement, the rod sleeve is inserted on a mounting plate through a connecting sheet, and the mounting plate is connected with the lifting driving assembly in a mounting way.
As an improvement, the edge of the two sides of the connecting sheet in the length direction is provided with a step-shaped inserting part for inserting connection.
As an improvement, a drawing end part for drawing the rod sleeve is arranged at one end part of the connecting sheet in the length direction.
As an improvement, the lifting driving assembly comprises two groups of parallel first driving units and second driving units which are vertically arranged;
the first driving unit and the second driving unit comprise motors, screw rod groups and connecting plates, and the motors drive the screw rod groups to operate and drive the corresponding magnetic rods or rod sleeves to lift and move through the connecting plates.
As an improvement, the horizontal driving assembly comprises an electric sliding table unit and a sliding table;
the electric sliding table unit is horizontally arranged;
the sliding table is driven by the electric sliding table unit to slide reciprocally along the horizontal direction, and the lifting driving assembly is arranged on the sliding table.
As an improvement, the electric sliding table unit synchronously drives the anti-drip plate to move through the sliding table.
As an improvement, the anti-drip plate moves relative to the sliding table through an independent driving unit, and the independent driving unit comprises a belt transmission module, a moving block, a sliding rail module, a positioning sensor and a positioning plate;
the belt of the belt transmission module drives the moving block to move, so that the drip-proof plate is driven to independently move;
the slide rail module is used for guiding and supporting the drip-proof plate;
the positioning inductors are arranged at two ends of the belt transmission module;
the positioning plate moves along with the anti-drip plate independently and moves to the corresponding positioning sensor to prompt the anti-drip plate to move in place.
The utility model has the beneficial effects that:
(1) According to the utility model, the lifting driving assembly is utilized to drive the magnetic rod and the rod sleeve to lift and move respectively, so that the lifting adjustment of the magnetic rod and the rod sleeve is independent, and meanwhile, the magnetic rod and the rod sleeve can be smoothly inserted and separated, so that the magnetic beads can be adsorbed and can be quickly separated;
(2) According to the utility model, the rod sleeves which are arranged in a straight line are arranged into a whole, so that the rod sleeves can be directly penetrated on the mounting plate, the mounting data of the rod sleeves are more accurate, the direct matching precision of the rod sleeves and the magnetic rods can be more accurate, and the mounting is more convenient;
(3) The utility model also provides the anti-drip plate, and the anti-drip plate is utilized to shield the container which is not subjected to magnetic bead purification, so that the other containers can not be polluted when the magnetic rod and the rod sleeve are combined to adsorb the magnetic beads.
In conclusion, the utility model has the advantages of high automation degree, simple separation and purification process, low pollution, high matching precision and the like, and is particularly suitable for the technical field of DNA plasmid magnetic bead purification and extraction.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the present utility model;
FIG. 2 is a schematic diagram of a lift driving assembly;
FIG. 3 is a second schematic diagram of the lift driving assembly;
FIG. 4 is a schematic diagram of a connection structure of a magnetic rod and a mounting base;
FIG. 5 is a schematic view of the connection structure of the rod sleeve and the mounting plate;
FIG. 6 is a schematic view of the overall structure of the stick sleeve;
FIG. 7 is a schematic diagram of a horizontal drive assembly;
FIG. 8 is a schematic diagram of the structure of an independent drive unit;
FIG. 9 is a schematic diagram of the connection structure of the drip plate and the independent driving unit.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
In the description of the present utility model, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings are merely for convenience in describing the present utility model and simplifying the description, and do not indicate or imply that the apparatus or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model.
Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present utility model, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.
Embodiment one:
as shown in fig. 1 to 9, a magnetic bead adsorption mechanism for DNA plasmid purification comprises:
the magnetic rods 1 and the rod sleeves 2 are arranged in a one-to-one correspondence manner, the magnetic rods 1 and the rod sleeves 2 are respectively lifted and arranged along the vertical direction through a lifting driving assembly 3, and the magnetic rods 1 adsorb magnetic beads through the rod sleeves 2;
the bottom of the lifting driving component 3 is provided with a horizontal driving component 4 which drives the magnetic rod 1 and the rod sleeve 2 to synchronously perform horizontal movement switching.
Further, the magnetic rod 1 comprises a rod body 11 and a magnetic suction head 12;
the rod body 11 is connected with the lifting driving assembly 3 through a mounting seat 13 arranged at the top;
the magnetic head 12 is located at the bottom of the barrel 11, and the magnetic head 12 has a magnetic force.
Further, a plurality of rod sleeves 2 are integrally arranged in a straight line, and the rod sleeves 2 comprise sleeve bodies 21 and connecting pieces 22.
Wherein, stick cover 2 inserts through connection piece 22 and locates mounting panel 23, and this mounting panel 23 is connected with lifting drive assembly 3 installation.
As shown in fig. 6, the two side edges of the connecting piece 22 in the length direction are respectively provided with a step-shaped inserting portion 221 for inserting connection, and the inserting portion 221 is matched with a slot on the mounting plate 23, so that the purpose of quickly mounting the rod sleeve is achieved.
In addition, for convenience in installation, a drawing end 222 for drawing the rod sleeve 2 is provided at one end of the connecting piece 22 in the longitudinal direction.
It should be noted that, through utilizing lift drive assembly 3 to drive bar magnet 1, stick cover 2 respectively and go up and down to remove for bar magnet 1 and stick cover 2 lift adjustment are independent, simultaneously, bar magnet 1 and stick cover 2 can be smooth and easy interlude and break away from, make the magnetic bead both can be adsorbed, also can make the magnetic bead break away from fast.
Further, the rod sleeves 2 are arranged in a straight line, so that when the rod sleeves 2 are installed, a plurality of groups of rod sleeves can be installed at one time, the installation efficiency is improved, and the installation accuracy of the rod sleeves is ensured.
As a preferred embodiment, the lifting driving assembly 3 includes two sets of parallel first driving units 31 and second driving units 32 disposed vertically;
the first driving unit 31 and the second driving unit 32 each comprise a motor 301, a screw rod set 302 and a connecting plate 303, and the motor 301 drives the screw rod set 302 to operate and drive the corresponding magnetic rod 1 or rod sleeve 2 to lift through the connecting plate 303.
Further, as shown in fig. 7, the horizontal driving assembly 4 includes an electric sliding table unit 41 and a sliding table 42;
the electric slipway unit 41 is horizontally arranged;
the sliding table 42 is driven by the electric sliding table unit 41 to slide reciprocally in the horizontal direction, and the lifting driving assembly 3 is mounted on the sliding table 42.
When the magnetic rod 1 and the rod sleeve 2 are matched, the magnetic rod can be used for adsorbing magnetic beads or releasing the magnetic beads, but the magnetic rod 1 and the rod sleeve 2 are required to be moved and switched in the horizontal direction because different containers are required to be switched, and the magnetic rod 1, the rod sleeve 2 and the lifting driving assembly 3 are synchronously adjusted in a sliding mode in the horizontal direction by the driving of the horizontal driving assembly 4.
In order to avoid pollution during purification, the electric sliding table unit 41 synchronously drives the anti-drip plate 5 to move through the sliding table 42.
Further, as shown in fig. 8 and 9, the drip-preventing plate 5 moves relative to the sliding table 42 by an independent driving unit 51, and the independent driving unit 51 includes a belt transmission module 511, a moving block 512, a sliding rail module 513, a positioning sensor 514 and a positioning plate 515;
the belt of the belt transmission module 511 drives the moving block 512 to move, so as to drive the drip-proof plate 5 to independently move;
the slide rail module 513 guides and supports the drip-proof plate 5;
the positioning sensors 514 are arranged at two ends of the belt transmission module 511;
the positioning plate 515 moves independently with the drip-preventing plate 5, and moves to the corresponding positioning sensor 514 to prompt the drip-preventing plate 5 to move in place.
It should be noted that, in order to avoid bar magnet and stick cover in removing the lift in-process, the solution that is stained with drops at will in other containers, leads to in DNA plasmid purification process, the circumstances of pollution appear, and this application shelters from the container through setting up antidrip liquid board 5 for when bar magnet 1 carries out the purification work of DNA plasmid to a set of container, the container that is close to the bar magnet is all the other is sheltered from, can not drop into solution.
Moreover, the drip-proof plate 5 in the present application can move along with the electric sliding table unit 41, and can also be moved and switched by the provided independent driving unit 51, so that the drip-proof plate is more independent and adjustable.
The foregoing description of the preferred embodiments of the utility model is not intended to be limiting, but rather is intended to cover all modifications, equivalents, and alternatives falling within the spirit and principles of the utility model.
Claims (8)
1. A magnetic bead adsorption mechanism for DNA plasmid purification, comprising:
the magnetic rods (1) and the rod sleeves (2) are arranged in a one-to-one correspondence mode, the magnetic rods (1) and the rod sleeves (2) are respectively lifted along the vertical direction through a lifting driving assembly (3), and the magnetic rods (1) conduct magnetic bead adsorption work through the rod sleeves (2);
the bottom of the lifting driving component (3) is provided with a horizontal driving component (4) for driving the magnetic rod (1) and the rod sleeve (2) to synchronously perform horizontal movement switching;
the rod sleeves (2) are integrally arranged in a straight line, and the rod sleeves (2) comprise sleeve bodies (21) and connecting sheets (22);
the two side edges of the connecting sheet (22) in the length direction are provided with ladder-shaped inserting parts (221) for inserting connection.
2. The magnetic bead adsorbing mechanism for purifying a DNA plasmid according to claim 1, wherein:
the magnetic rod (1) comprises a rod body (11) and a magnetic suction head (12);
the rod body (11) is connected with the lifting driving assembly (3) in a mounting way through a mounting seat (13) arranged at the top;
the magnetic suction head (12) is positioned at the bottom of the rod body (11), and the magnetic suction head (12) has magnetic force.
3. The magnetic bead adsorbing mechanism for purifying a DNA plasmid according to claim 1, wherein:
the rod sleeve (2) is inserted on a mounting plate (23) through a connecting sheet (22), and the mounting plate (23) is connected with the lifting driving assembly (3) in a mounting way.
4. The magnetic bead adsorbing mechanism for purifying a DNA plasmid according to claim 1, wherein:
one side end part of the connecting sheet (22) in the length direction is provided with a drawing end part (222) for drawing the rod sleeve (2).
5. The magnetic bead adsorbing mechanism for purifying a DNA plasmid according to claim 1, wherein:
the lifting driving assembly (3) comprises two groups of parallel first driving units (31) and second driving units (32) which are vertically arranged;
the first driving unit (31) and the second driving unit (32) comprise motors (301), screw rod groups (302) and connecting plates (303), and the motors (301) drive the screw rod groups (302) to operate and drive the corresponding magnetic rods (1) or rod sleeves (2) to lift through the connecting plates (303).
6. The magnetic bead adsorbing mechanism for purifying a DNA plasmid according to claim 1, wherein:
the horizontal driving assembly (4) comprises an electric sliding table unit (41) and a sliding table (42);
the electric sliding table unit (41) is horizontally arranged;
the sliding table (42) is driven by the electric sliding table unit (41) to slide reciprocally along the horizontal direction, and the lifting driving assembly (3) is arranged on the sliding table (42).
7. The magnetic bead adsorbing mechanism for purifying a DNA plasmid according to claim 6, wherein:
the electric sliding table unit (41) synchronously drives the anti-drip plate (5) to move through the sliding table (42).
8. The magnetic bead adsorbing mechanism for purifying a DNA plasmid according to claim 7, wherein:
the anti-drip plate (5) moves relative to the sliding table (42) through an independent driving unit (51), and the independent driving unit (51) comprises a belt transmission module (511), a moving block (512), a sliding rail module (513), a positioning sensor (514) and a positioning plate (515);
the belt of the belt transmission module (511) drives the moving block (512) to move, so that the drip-proof plate (5) is driven to move independently;
the slide rail module (513) guides and supports the anti-drip plate (5);
the positioning inductors (514) are arranged at two ends of the belt transmission module (511);
the positioning plate (515) moves along with the anti-drip plate (5) independently and moves to the corresponding positioning sensor (514) to prompt the anti-drip plate (5) to move in place.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
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| CN2022222465720 | 2022-08-25 | ||
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| CN219260002U true CN219260002U (en) | 2023-06-27 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116751674A (en) * | 2023-08-22 | 2023-09-15 | 广州凯普医药科技有限公司 | Magnetic bead method DNA draws and DNA methylation conversion purification device |
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- 2022-11-23 CN CN202223110041.5U patent/CN219260002U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116751674A (en) * | 2023-08-22 | 2023-09-15 | 广州凯普医药科技有限公司 | Magnetic bead method DNA draws and DNA methylation conversion purification device |
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