CN110650804A

CN110650804A - Biochemical reaction device and sleeve mechanism thereof

Info

Publication number: CN110650804A
Application number: CN201780090968.XA
Authority: CN
Inventors: 李俊明; 林清格; 蔡汮龙; 李珮瑜; 苏城; 张晓芬; 李馥君
Original assignee: Genereach Biotechnology Corp
Current assignee: Genereach Biotechnology Corp
Priority date: 2017-09-19
Filing date: 2017-09-19
Publication date: 2020-01-03
Also published as: WO2019056166A1

Abstract

A biochemical reaction device and a casing mechanism (10) thereof, the casing mechanism (10) comprises: the device comprises a first carrier (11), wherein at least one first through hole (111) is formed in the first carrier (11); at least one connecting piece (12), each connecting piece (12) is arranged corresponding to each first through hole (111), each connecting piece (12) is provided with a second through hole (123), the second through holes (123) are communicated with or overlapped with the first through holes (111) in a penetrating way, and each connecting piece (12) is also provided with a joint part (121); and at least one sleeve (13) having opposite ends, one of which is an open nose end (131), each sleeve being detachably connectable by the nose end (131) to the engagement portion (121) of the connector (12).

Description

Biochemical reaction device and sleeve mechanism thereof

Technical Field

The present disclosure relates to a biochemical reaction device, and more particularly, to a casing mechanism capable of conveniently replacing a casing and preventing operational contamination, and a biochemical reaction device including the casing mechanism.

Background

Biochemical reactions commonly used in biotechnology include nucleic acid extraction, Polymerase Chain Reaction (PCR), complementary nucleic acid hybridization, or immunological binding. By using the techniques and reactions, nucleic acid can be extracted from cells, viruses, bacteria or other biological tissues, and then a specific or to-be-detected target nucleic acid sequence is amplified by using a polymerase chain reaction, and the amplified nucleic acid product can be subjected to a nucleic acid complementary binding reaction by using a primer connected with an identification marker in the amplification process, or subjected to a hybridization reaction with a complementary nucleic acid sequence connected with the identification marker, so that whether the target nucleic acid sequence or the amplified nucleic acid product exists or the quantity of the target nucleic acid sequence or the amplified nucleic acid product can be determined. In addition, for the identification of protein or oligopeptide, the purpose of qualitative or quantitative determination can be achieved by utilizing the immune reaction of the antibody with specific binding property and also by the identification mark connected with the antibody.

However, in the operation of these reactions, if the number of samples to be processed is large, the operator will repeatedly add or remove the reaction solution or product from each sample, which is not only a heavy burden, but also makes it easier to mistake the sample, add a wrong solution, add more or less solution during the operation process, or pollute the sample due to many operation steps, so that the reaction solution has operation errors, and the accuracy of the reaction result is affected.

Therefore, in order to overcome and reduce the operational errors, many automated biochemical reaction devices have been manufactured and applied, such as an automated PCR device, an automated nucleic acid extraction device, etc. The biochemical reaction devices perform the steps of adding and pipetting the reaction solution through automatic mechanisms such as mechanical arms or displacement mechanisms, and adjust the reaction conditions such as temperature, reaction time and the like through temperature control components, thereby reducing the burden of operators and improving the accuracy of the reaction. However, even with an automated device, it is currently not possible to automate each step, and manual intervention is still required in some of the steps. For example: before the reaction operation, reaction tubes for reaction are installed to inject the required reaction solution into the reaction tubes for subsequent nucleic acid amplification reaction, or the reaction tubes are used in conjunction with other magnetic attraction mechanisms to perform the extraction reaction of nucleic acid. In general, in the aforesaid automatic reaction device, in order to operate and react multiple samples together, a plurality of parallel actuating mechanisms are usually provided, and the reaction tubes used for the reaction are generally made of a plurality of tubes connected with the reaction tubes, and in order to install the tubes, the operating arm of the biochemical device is provided with corresponding sliding grooves for the tubes to be inserted and slid to be positioned

Although the arrangement of the calandria can achieve the purpose of installing a plurality of reaction tubes simultaneously, in the manual installation process, the calandria is often polluted to affect the subsequent reaction. Similarly, after the reaction is finished, the calandria must be manually pulled out or replaced, which is also likely to cause the problem of cleaning the inside of the device. In addition, about eight or twelve reaction tubes are usually connected to the rack pipe, and if the number of samples is not large, one unit of rack pipe still needs to be used, so that the required number of reaction tubes cannot be installed as required, and the material cost is relatively increased.

Disclosure of Invention

The present disclosure is directed to a sleeve mechanism of a biochemical reaction apparatus, which does not require manual installation and removal of a reaction tube, so that the reaction tube can be automatically installed in the biochemical reaction apparatus, thereby preventing contamination of a sample due to manual installation.

Another objective of the present disclosure is to provide a sleeve mechanism of a biochemical reaction device capable of easily engaging with a reaction tube, wherein the sleeve mechanism can easily engage with the reaction tube by a special engaging structure corresponding to the connecting member and the reaction tube or/and a gap formed on the reaction tube, so as to simultaneously position the engaging position of the reaction tube and avoid the disadvantage of difficulty in disengaging or failing to disengage when the mechanism in the related art retracts.

It is another object of the present disclosure to provide a sleeve mechanism of a biochemical reaction apparatus, which is applicable to different reaction tube specifications, wherein the connecting member can be detachably disposed, and the corresponding connecting member can be replaced according to the specification of the reaction tube, so that the sleeve mechanism can be applied to the reaction of various reaction tubes or reaction plates.

Another objective of the present disclosure is to provide a casing mechanism of a biochemical reaction apparatus capable of individually installing a desired number of reaction tubes according to requirements without installing a rack tube as in the related art, thereby reducing the cost of reaction tube consumables.

In order to achieve the above object, the present disclosure provides a casing mechanism of a biochemical reaction apparatus, including: the first carrying piece is provided with at least one first through hole; at least one connecting piece, each connecting piece is arranged corresponding to each first through hole, each connecting piece is provided with a second through hole, the second through holes are communicated with or overlapped with the first through holes in a penetrating way, and each connecting piece is provided with a joint part; and at least one sleeve (reaction tube) having opposite ends, one of which is an open nose end, each of the sleeves being detachably connectable by the nose end to the junction of the connector.

In an embodiment of the disclosure, the sleeve mechanism of the biochemical reaction device may further include a locking portion that is locked with the corresponding engaging portion of the connecting member when the sleeve is connected. In the embodiment of the present disclosure, the engaging portion is an annular protruding structure disposed on an outer edge of the connecting member, and the engaging portion is a corresponding groove structure, but not limited thereto. The joint part and the clamping part can be interchanged in the structure, namely, the joint part can be a groove structure arranged on the outer edge of the connecting piece, and the clamping part is a corresponding annular protruding structure; or other structures which can be temporarily connected and separated and have corresponding clamping, tight fitting and embedding.

In an embodiment of the disclosure, in the casing mechanism of the biochemical reaction apparatus, each of the connecting members may be detachably mounted below the first carrier corresponding to each of the first through holes, and the second through hole of the connecting member is communicated with the first through hole.

In the embodiment of the disclosure, each of the connecting elements may also be detachably embedded in the first through hole, and the joint portion is exposed below the first carrier, at this time, the second through hole of the connecting element overlaps with the first through hole.

In the embodiment of the disclosure, the connecting pieces can be replaced by corresponding connectable structures according to the specification of the connected sleeve.

In an embodiment of the disclosure, the casing mechanism of the biochemical reaction apparatus, wherein the first carrier may further include a cover plate for limiting the connecting members, and the cover plate may further include an inserting hole corresponding to and communicating with the first through hole.

In an embodiment of the disclosure, the sleeve mechanism of the biochemical reaction device, wherein the sleeve is further provided with a rib at an outer edge below the latching portion, so that the sleeves can be placed on a sleeve rack or a reaction disk in advance, and then automatically connected to the connecting member by the sleeve mechanism of the embodiment of the disclosure.

In the embodiment of the present disclosure, the sleeve has at least one gap formed at an end edge of the tube opening end, and the gap may be formed approximately parallel to the axial direction of the sleeve, but is not limited thereto.

In another embodiment of the present disclosure, there is provided a biochemical reaction apparatus for reacting a solution in a reaction disk, including: the magnetic attraction mechanism comprises a second carrying piece, at least one reaction rod is arranged on the second carrying piece, and the reaction rod is arranged corresponding to the first through hole; the sleeve mechanism and the magnetic attraction mechanism are respectively connected with a longitudinal displacement mechanism, when the magnetic attraction mechanism longitudinally displaces downwards, the reaction rods arranged on the magnetic attraction mechanism can penetrate into the sleeve through the first through holes, and a top end of each reaction rod moves to a preset position in the sleeve.

In an embodiment of the disclosure, after the reaction rod moves to the predetermined position, the magnetic attraction mechanism may operate together with the sleeve mechanism, so that the sleeve and the reaction rod penetrating therethrough move into or out of at least one hole groove of the reaction disk together for reaction.

In an embodiment of the present disclosure, the reaction rod may be a magnetic rod, which may be a permanent magnet or an electromagnet, but not limited thereto.

According to the sleeve mechanism and the biochemical reaction device provided by the disclosure, the sleeves can be prepared according to the required number and then automatically connected by the sleeve mechanism, so that the cost problem of sleeve use can be improved, and the pollution problem caused by manual operation can be avoided. In addition, through this disclosure, can make the sleeve pipe cup joint on the connecting piece easily to reach the purpose of location and easy withdrawal of tubes. Meanwhile, the connecting piece can be detachably arranged, so that the sleeve pipe joint can be applied to sleeve pipes with different specifications.

The following examples are presented to illustrate the present disclosure and not to limit the scope of the disclosure, which is to be determined by the appended claims as may be amended by those skilled in the art without departing from the scope and spirit of the present disclosure.

Drawings

FIG. 1 is an assembled schematic view of an embodiment of a biochemical reaction device including an embodiment of a cannula mechanism of the present disclosure.

Fig. 2 is a schematic perspective view and a partially enlarged view of an embodiment of a cannula mechanism of the present disclosure.

FIG. 3 is a schematic perspective view of another angle of an embodiment of a cannula mechanism of the present disclosure.

FIG. 4 is a schematic diagram of the actuation of the sleeve mechanism and the magnetic attraction mechanism according to the embodiment of the disclosure.

FIG. 5 is a schematic side view of the sleeve mechanism and the magnetic attraction mechanism according to the embodiment of the disclosure.

FIG. 6 is a schematic view of an embodiment of a magnetic attraction mechanism of the present disclosure with a reaction rod inserted into a cannula mechanism.

Detailed Description

Referring to FIG. 1, the assembly of the biochemical reaction apparatus including the cannula mechanism according to the embodiment of the present disclosure is shown. The biochemical reaction device that this disclosed embodiment provided includes: the device comprises a sleeve mechanism 10, a magnetic attraction mechanism 20, a reaction disc 30, a base 40 and a displacement mechanism 50. The sleeve mechanism 10 and the magnetic attraction mechanism 20 are respectively connected to a longitudinal displacement mechanism (not shown) disposed on the base 40, and can be vertically displaced relative to the base 40. The reaction tray 30 can be horizontally and laterally displaced on the bottom platform of the susceptor 40 by a lateral displacement mechanism 50. By the operation of the sleeve mechanism 10, the sleeve 13 as a reaction tube can be connected to the sleeve mechanism 10 under the automatic operation, and then the sleeve 13 can be automatically moved into or out of the hole 31 of the reaction tray 30 according to the setting by the operating mechanism of the sleeve mechanism 10 and the magnetic attraction mechanism 20. In this embodiment, the automatic nucleic acid extracting apparatus is taken as an example, and the magnetic beads and the magnetic attraction principle are used to extract nucleic acids, so the magnetic attraction mechanism 20 is provided to operate, and if the reaction solution is simply added to the casing to perform, for example, a nucleic acid amplification or hybridization reaction, the magnetic attraction mechanism 20 may not be provided.

Referring to fig. 2 and fig. 3, fig. 2 and fig. 3 are schematic perspective views of the sleeve mechanism according to the embodiment of the present disclosure. The sleeve mechanism 10 comprises a first carrier 11, a connecting member 12 and a sleeve 13. The connecting element 12 is mounted on the first carrier element 11 and is connected to the sleeve 13.

The first carrier 11 is an elongated plate in this embodiment, but the structure is not particularly limited. The first carrier 11 is provided with a plurality of first through holes 111, and the number of the first through holes 111 can be set according to the number of the holes in a row of the common reaction disk. The first carrier 11 can be connected to a first extension 14 and connected to a longitudinal displacement mechanism (not shown) through a first connection 15 provided thereon, or directly connected to the longitudinal displacement mechanism through a first connection 15 provided thereon. The first connecting portion 15 connects and fixes only the first carriage 11 to the longitudinal displacement mechanism, and is not particularly limited, and may be detachably connected by a screw, or may be connected by welding, clamping, and then adhering.

The connecting members 12 can be directly mounted under the first carrier 11 corresponding to the first through holes 111, or by using a embedding method, after a step-shaped embedding groove (not shown) is formed in the first through holes 111, the connecting members 12 are respectively inserted into the first through holes 111 for embedding. Each connecting element 12 is provided with a second through hole 123, if the connecting element 12 is installed below the first carrier 11, the second through hole 123 and the first through hole 111 penetrate and can be communicated with each other, if the connecting element 12 is installed in the first through hole 111 in a embedding manner, at this time, the second through hole 123 is overlapped and arranged in the first through hole 111 and extends out of the first through hole 111. In the embodiment, the connecting element 12 is installed in an embedding manner, in order to limit the connecting element 12 in the first through hole 111, a cover plate 112 may be further installed on the first carrier 11, the cover plate 112 is further provided with an inserting hole 113 corresponding to the first through hole 111/the second through hole 123, and the inserting hole 113 and the second through hole 123 are communicated with each other. The connecting member 12 may be detachably assembled on the first carrier 11, such as by clipping, locking, and embedding, without any particular limitation.

The connecting member 12 has a joint portion 121 and a base portion 122 on the outer peripheral surface. The connecting part 12, no matter being installed under the first carrier 11 or embedded in the first through hole 111, needs to be exposed under the first carrier 11 to connect with the sleeve 13. The engaging portion 121 is an annular protruding structure in the embodiment, but not limited thereto, and may also be a circular convex structure, a circular concave groove, a circular concave structure, or a snap structure corresponding to the latching portion 132. The base portion 122 is disposed above the engaging portion 121, is located between the engaging portion 121 and the first carrier 11, and can be used as a limiting structure for the end of the sleeve 13 to abut against after being connected with the sleeve 13.

The sleeve 13, i.e. the reaction tube for reaction, can be used as a test tube for containing the reaction solution, or as one of the reaction mechanisms of the magnetic attraction mechanism 20 in this embodiment. In this embodiment, one end of the sleeve 13 is open, and the opposite end is a closed tube structure, and the opposite end may be open according to the requirement, so the present invention is not limited thereto; the length and the pipe diameter of the pipe body can be selected according to the requirement of the reaction volume and the specification of the hole groove 31 arranged on the reaction disc 30. The open end of the sleeve 13 is a tube opening end 131, and a locking portion 132 is disposed on the inner edge of the sleeve 13 near the tube opening end 131. The latch 132 is connected to the engaging portion 121 when the sleeve 13 is connected to the connecting member 12. Therefore, the locking portions 132 are connected to each other corresponding to the engaging portions 121, and may be a groove, an annular groove structure, a circular protrusion, a bump, an annular protrusion structure, or a locking structure corresponding to the engaging portions 121. The opening end 131 of the sleeve 13 may be opened with at least one gap 1311, which is similar to a groove in shape but not limited thereto, from the opening end 131 as in this embodiment, so that when the connecting member 12 is pushed against the opening end 131 to be sleeved, the opening end 131 can be slightly expanded due to the gap 1311, and the connecting member 12 can more easily pass through the opening end 131 to be sleeved in the sleeve 13. The gaps 1311 may be formed approximately parallel to the axial direction of the sleeve 13, but the number of the gaps is not limited thereto, and may be adjusted according to the pipe diameter or the hardness of the material of the sleeve. The sleeve 13 may further have a rib 133 at the outer edge under the latch 132 for placing the sleeve 13 on the placing hole (not shown) of the reaction disk 30 or the sleeve frame, and then connecting with the sleeve mechanism 10. The rib 133 may be annularly provided, or may be provided in plurality and arranged in an annular distribution, and the shape thereof is not particularly limited.

When the sleeve 13 is connected, the reaction disk 30 or the sleeve frame (in which the sleeve 13 is placed on the hole 31 of the reaction disk 30 through the rib 133 and is not reacted or on the placing hole of the sleeve frame, and then is sealed and packaged) with the required number of sleeves is prepared, the sleeve 13 is placed on the transverse displacement mechanism 50 after being torn, and the transverse displacement mechanism 50 and the longitudinal displacement mechanism connected with the sleeve mechanism 10 are operated at the same time, so that the connecting piece 12 of the sleeve mechanism 10 is displaced to the upper side of the pipe orifice end 131 of the sleeve 13, and then is further displaced downwards to abut against, so that the joint part 121 on the connecting piece 12 is engaged with the engaging part 132 of the sleeve 13, and subsequent reaction action can be performed after engagement. When the sleeve 13 is sleeved on the connecting member 12 in a sleeving manner, the end of the pipe orifice end 131 of the sleeve 13 may be made of plastic or other materials with slightly deforming characteristics, and may be slightly deformed when the connecting member 12 abuts against, so that the connecting member 12 can further extend into the sleeve 13, and the engaging portion 121 is connected with the locking portion 132.

The automatic nucleic acid extractor with magnetic attraction reaction is to add modified magnetic micro beads or magnetically attracted micro beads into the treated sample hole, the modified micro beads can adhere to the nucleic acid released from the sample after reaction, then pass through a sleeve pipe, install a magnetic rod in the sleeve pipe, the two are immersed in the solution with micro beads, the micro beads adhered with nucleic acid are adsorbed on the outer wall of the sleeve pipe by magnetic attraction, then the sleeve pipe with adsorbed micro beads is moved to another row or several rows of hole grooves for cleaning reaction, the impurities except nucleic acid are cleaned and removed, then the sleeve pipe is moved to another row of hole grooves to separate micro beads from nucleic acid, thus obtaining purified nucleic acid, and the micro beads adsorbed on the sleeve pipe can be recovered for reuse after the magnetic rod is removed.

Referring to fig. 4 and 5 in conjunction with fig. 1, fig. 4 and 5 are schematic views illustrating the operation of the sleeve mechanism and the magnetic attraction mechanism according to the embodiment of the present disclosure. In order to achieve the above mentioned nucleic acid extraction reaction by magnetic attraction, the biochemical reaction apparatus of the present disclosure is further provided with a magnetic attraction mechanism 20, which includes: a second carrier 21 and a reaction rod 22. The second carrier 21 is an elongated plate/block in this embodiment, but the structure thereof is not particularly limited. The second carrier 21 is provided with a plurality of reaction rods 22 thereunder. The reaction rods 22 are provided in numbers and positions corresponding to the insertion holes 113 (or the first through holes 111/the second through holes 123) in the casing mechanism 10. The second carrier 21 can be connected to a second extension 23 and connected to a longitudinal displacement mechanism (not shown) via a second connection 24 provided thereon, or directly connected to the longitudinal displacement mechanism via a second connection 24 provided thereon. The second connecting portion 24 is only for connecting and fixing the second carrier 21 to the longitudinal displacement mechanism, and is not particularly limited, and may be detachably connected by a screw, or may be connected by welding, clamping, and adhering. The reaction rod 22 is a magnetic rod in this embodiment, and may be a permanent magnet or electromagnet type rod.

Please refer to fig. 6, which is a schematic diagram of a magnetic attraction mechanism according to an embodiment of the present disclosure, wherein a reaction rod is inserted into a sleeve mechanism. The sleeve mechanism 10 and the magnetic attraction mechanism 20 are respectively connected to a longitudinal displacement mechanism (not shown), which can be individually or simultaneously operated according to the setting. Before the magnetic attraction, the magnetic attraction mechanism 20 moves downward to make the reaction rod 22 penetrate into the operation cavity 130 of the sleeve 13 through the insertion hole 113 (or the first through hole 111/the second through hole 123), and move the top end 221 of the end of the reaction rod 22 to a predetermined position in the operation cavity 130, where the predetermined position can be selected according to the configuration of the top end 221 and the configuration of the bottom 134 of the operation cavity 130, and there is no special limitation, and generally, a predetermined position close to the bottom 134 but not yet touched is preferred, so that on one hand, a better magnetic attraction force can be provided for the bottom of the sleeve 13, and on the other hand, the sleeve 13 can be prevented from being loosened from the connecting member 12 due to the touch of the reaction rod 22.

When the reaction rod 22 of the magnetic attraction mechanism 20 is displaced to a predetermined position, the magnetic attraction mechanism 20 and the sleeve mechanism 10 are actuated together, and together with the transverse displacement mechanism 50, the sleeve 13 together with the reaction rod 22 inserted therein is displaced to a position above the hole array groove 31 to be magnetically attracted (see fig. 1 at the same time), and then moves down into the hole array groove 31 together, and adsorbs the microbeads adhered with nucleic acids in the solution onto the outer edge tube wall of the sleeve 13, and then moves up away from the hole array groove 31 together. After moving up, the reaction tray 30 is displaced by a row of hole-groove distance by the transverse displacement mechanism 50, so that the sleeve 13/the reaction rod 22 is positioned above the next row of hole grooves, and then moves down to enter the next row of hole grooves 31 for cleaning reaction, the cleaning reaction can be performed by setting different rows of cleaning solution according to requirements, the cleaning steps of the rows are repeated, and finally, the reaction tray enters the hole grooves containing the solution capable of separating the nucleic acid from the microbeads, and the nucleic acid can be recovered and purified.

By the above-mentioned sleeve mechanism 10 and the operation of the magnetic attraction mechanism 20, the sleeve 13 can be automatically and easily connected to the connector 12 by the operation of the device in the initial step, so as to avoid the problem of contamination of the outer edge of the wall of the sleeve 13 caused by manual installation of the sleeve 13, and the nucleic acid extraction procedure can greatly reduce the intervention of contamination of external samples, so that the reaction result can be more accurate or the generation of false positive can be reduced during the subsequent PCR reaction. On the other hand, if the number of samples to be tested or reacted is small, the corresponding number of sleeves can be prepared according to the requirement, so that the purpose of reducing the cost of consumable materials can be achieved.

Claims

A casing mechanism of a biochemical reaction apparatus, comprising:

the first carrying piece is provided with at least one first through hole;

at least one connecting piece, each connecting piece is arranged corresponding to each first through hole, each connecting piece is provided with a second through hole, the second through holes are communicated with or overlapped with the first through holes in a penetrating way, and each connecting piece is provided with a joint part; and

at least one sleeve with two opposite ends, wherein one end is an open pipe end, each sleeve is provided with a clamping part, and the pipe end can be detachably clamped with the corresponding joint part on the connecting piece;

the connecting part is an annular protruding structure arranged on the outer edge of the connecting piece, and the clamping part is a corresponding groove structure, or the connecting part is a groove structure arranged on the outer edge of the connecting piece, and the clamping part is a corresponding annular protruding structure.
The casing mechanism for biochemical reaction apparatus according to claim 1, wherein each of the connecting members is detachably mounted below the first carrier corresponding to each of the first through holes, and the second through hole of the connecting member is in through communication with the first through hole.
The casing mechanism for biochemical reaction apparatus according to claim 1, wherein each of the connecting members is detachably embedded in the first through hole such that the engaging portion is exposed below the first carrier, and the second through hole of the connecting member overlaps with the first through hole.
The casing mechanism of biochemical reaction apparatus according to claim 3, wherein the first carrier is further provided with a cover plate for limiting the connecting members, the cover plate is provided with an inserting hole corresponding to and communicating with the first through hole.
The biochemical reaction device according to claim 1, wherein the sleeve is further provided with a rib at an outer edge below the detent.
The casing mechanism of biochemical reaction apparatus according to claim 1, wherein the casing has at least one gap at an end edge of the nozzle end.
A biochemical reaction apparatus for reacting a solution in a reaction plate, comprising:

a cannula device according to any of claims 1 to 6; and

the magnetic suction mechanism comprises a second carrying piece, at least one reaction rod is arranged on the second carrying piece, and the reaction rod is arranged corresponding to the first through hole;

the sleeve mechanism and the magnetic attraction mechanism are respectively connected with a longitudinal displacement mechanism, when the magnetic attraction mechanism longitudinally displaces downwards, the reaction rods arranged on the magnetic attraction mechanism can penetrate into the sleeve through the first through holes, and the top ends of the reaction rods move to preset positions in the sleeve.
The biochemical reaction apparatus according to claim 7, wherein after the reaction rod moves to the predetermined position, the magnetic attraction mechanism can be actuated together with the sleeve mechanism to move the sleeve and the reaction rod inserted therein into or out of the at least one hole of the reaction tray for reaction.
The biochemical reaction apparatus according to claim 7 or 8, wherein the reaction rod is a rod body that can generate magnetism.
The biochemical reaction device according to claim 9, wherein the reaction rod is a permanent magnet rod body or an electromagnet rod body.