CN219861289U - Nucleic acid extraction tray combination - Google Patents

Abstract

A nucleic acid extraction tray assembly comprises an extraction reagent well tray and an anti-fouling barrier disposed above the extraction reagent well tray. The extraction reagent well plate is provided with a plurality of well groups for performing nucleic acid extraction operations of a plurality of samples, each well group comprises a plurality of well grooves which are arranged in a straight line, and each well groove contains a reagent required by the nucleic acid extraction operations. The anti-fouling baffle comprises a plurality of containing holes, a plurality of anti-fouling baffles and a plurality of shielding laminates. The accommodating hole accommodates the magnetic rod sleeve. The antifouling baffle separates a plurality of spaces respectively corresponding to a plurality of hole groove groups above the extraction reagent hole tray. The shielding layer plate is arranged in each space in the direction perpendicular to the antifouling baffle. During the nucleic acid extraction operation, the end of the magnetic rod sleeve to which the reagent is attached moves within the space, and the joint of the opposite ends of the magnetic rod sleeve moves over the shielding layer.

Description

Nucleic acid extraction tray combination

Technical Field

The present disclosure relates to a nucleic acid extraction tray assembly, and more particularly to a nucleic acid extraction tray assembly capable of preventing cross contamination.

Background

In the prior art, one of the common methods used for nucleic acid extraction is the magnetic bead method. The nucleic acid extraction technology of the magnetic bead method utilizes the specific recognition and efficient combination of magnetic bead particles and nucleic acid molecules to achieve nucleic acid extraction, and has the advantages of easy automation realization, simple flow, simple and convenient operation, short time consumption, safety, no toxicity, high purity of purified nucleic acid and the like. Because the magnetic beads can be gathered or dispersed under the magnetic field condition, the manual operation flow required by centrifugation and the like can be thoroughly eliminated, and the automatic nucleic acid extraction device developed on the market is mostly based on the principle of a magnetic bead method.

Generally, an automated nucleic acid extraction device has a plurality of magnetic rods disposed on a magnetic rod rack, which works together with an extraction reagent well plate (including an extraction well plate and an included nucleic acid extraction reagent) and a magnetic rod sleeve to extract and purify nucleic acids. The working principle is that magnetic beads adsorbed with nucleic acid are moved into different reagent hole grooves by utilizing a magnetic rod, reagent liquid is repeatedly and rapidly stirred by utilizing a magnetic rod sleeve sleeved on the outer layer of the magnetic rod, so that the reagent liquid and the magnetic beads are uniformly mixed, and the high-purity nucleic acid is finally obtained through the steps of cell lysis, nucleic acid adsorption, cleaning, elution and the like.

In order to ensure the extraction efficiency, the magnetic rod sleeve can vibrate up and down in the corresponding hole slots of the extraction reagent hole tray with certain intensity and frequency, but the method can generate aerosol and cause cross contamination of samples between the adjacent hole slots, so how to achieve the aim of reducing or avoiding cross contamination between samples while ensuring the nucleic acid extraction efficiency is really a problem to be solved. In addition, the existing automatic nucleic acid extraction device needs to be provided with a magnetic rod sleeve manually before use, which particularly increases the labor cost when detecting a large number of samples, so how to reduce the manual operation steps and the labor cost is a problem to be solved for improving the automatic operation efficiency.

Therefore, there is a need to develop a nucleic acid extraction tray assembly that avoids cross-contamination and reduces labor costs.

Disclosure of Invention

The present disclosure provides a nucleic acid extraction tray assembly that can be used in an automated nucleic acid extraction apparatus to ensure nucleic acid extraction efficiency while also avoiding cross-contamination between samples.

Another object of the present utility model is to provide a nucleic acid extraction tray assembly, which reduces the labor cost of installing and removing the magnetic rod sleeve by using a novel magnetic rod sleeve structure and presetting the magnetic rod sleeve in an anti-fouling baffle, effectively improves the automation efficiency, and simultaneously reduces the probability of pollution of the magnetic rod sleeve connector during the nucleic acid extraction operation, and further reduces the labor required for cleaning.

To achieve the above objective, the present utility model provides a nucleic acid extraction tray assembly, which comprises an extraction reagent well tray and an anti-fouling baffle. The extraction reagent well plate is provided with a plurality of well groups for performing nucleic acid extraction operation of a plurality of samples, wherein each well group comprises a plurality of well grooves which are arranged in a straight line, and each well groove is used for accommodating a reagent required by the nucleic acid extraction operation. The antifouling baffle is arranged above the extraction reagent hole tray and comprises a plurality of containing holes, a plurality of antifouling baffles and a plurality of shielding laminates. The plurality of holding holes are used for respectively holding a magnetic bar sleeve, the plurality of antifouling baffles are used for separating a plurality of spaces respectively corresponding to the plurality of hole groove groups above the extraction reagent hole tray, and the plurality of shielding laminates are correspondingly arranged in each space in the direction perpendicular to the plurality of antifouling baffles. Wherein the end portion of the bar magnet cover to which the reagent is attached moves within the space during the nucleic acid extraction operation, and the joint portion of the bar magnet cover with respect to the end portion moves above the shielding layer.

In one embodiment, the shielding layer is constructed such that the space is narrowed inward in a cross section perpendicular to the contamination barrier to form a through hole, and the through hole is for the end of the magnetic rod sleeve to pass through and move during the nucleic acid extraction operation.

In one embodiment, the shielding layer plate divides the space into a first space located below and a second space located above, and the joint of the magnetic rod sleeve moves in the second space during the nucleic acid extraction operation.

In one embodiment, the plurality of anti-fouling baffles are respectively corresponding to the groove walls between each adjacent hole groove group in the plurality of hole groove groups so as to form a plurality of spaces.

In an embodiment, the positions of the anti-fouling baffles corresponding to the plurality of anti-fouling baffles have a first height, and the anti-fouling baffles have a second height on one side of the plurality of anti-fouling baffles, and the second height is smaller than the first height.

In an embodiment, the engaging portion of the magnetic rod sleeve is used for being combined with the magnetic rod sleeve connecting piece, and a plurality of engaging pieces are arranged on the inner side of the engaging portion so as to be engaged with the engaging portions on the magnetic rod sleeve connecting piece.

In one embodiment, a plurality of protruding pieces are arranged on the outer side of the joint part of the magnetic rod sleeve.

In an embodiment, the magnetic rod sleeve further comprises a pipe body connecting the joint portion and the end portion, and the diameter of the joint portion is larger than the diameter of the pipe body and the diameter of the end portion.

In one embodiment, the sum of the lengths of the tube body and the end is equal to the sum of the height of the well and the distance between the shielding plate and the top edge of the extraction reagent well plate.

In an embodiment, the anti-fouling blocking member further includes a plurality of protection sleeves respectively disposed below each of the accommodating holes to protect the magnetic rod sleeve disposed in the accommodating hole.

In an embodiment, the extraction reagent well plate further includes at least one through groove for correspondingly disposing a plurality of protection sleeves and magnetic rod sleeves respectively disposed in the plurality of protection sleeves.

In one embodiment, the extraction reagent well plate has a plurality of first fasteners and the anti-fouling shield has a plurality of second fasteners, and the anti-fouling shield is fixed above the extraction reagent well plate by the first fasteners and the second fasteners being fastened to each other.

In one embodiment, the depth of the well used to perform an elution step is less than the depths of the other wells of the plurality of wells.

In one embodiment, the well has an inlet, a receiving space, and a bottom portion, and the bottom portion has a smaller cross-section in the longitudinal direction of the well than the other portions of the well.

In an embodiment, the anti-fouling device further comprises a protecting cover, and the protecting cover is arranged above the anti-fouling baffle.

In one embodiment, the extraction reagent well plate further comprises at least one sealing membrane for sealing the plurality of wells of the extraction reagent well plate.

Drawings

FIG. 1A is a schematic diagram of a nucleic acid extraction tray assembly according to an embodiment of the disclosure;

FIG. 1B is an exploded view of a nucleic acid isolation tray assembly according to an embodiment of the present disclosure;

FIG. 1C is a cross-sectional view of a nucleic acid isolation tray assembly according to an embodiment of the present disclosure;

FIG. 2A shows a top view of an extraction reagent well plate of an embodiment of the present disclosure;

FIG. 2B is a schematic diagram of an extraction reagent well plate according to another embodiment of the present disclosure;

FIG. 3 shows a top view of an anti-fouling barrier of an embodiment of the present disclosure;

FIG. 4 is a schematic diagram showing a nucleic acid extraction tray assembly according to another embodiment of the present disclosure;

FIG. 5 is a schematic diagram showing a combination of a magnetic rod sleeve, a magnetic rod sleeve connecting piece and a magnetic rod according to an embodiment of the present disclosure;

fig. 6 shows a schematic diagram of a combination of a magnetic rod sleeve and a magnetic rod sleeve connector according to an embodiment of the present disclosure.

[ symbolic description ]

1: nucleic acid extraction tray combination

11: extraction reagent hole tray

111: through groove

112: hole and groove group

113. 113a, 113b, 113c, 113d, 113e: hole groove

1131: an inlet

1132: accommodating space

1133: bottom portion

114: first fastener

115: marking position

116: sealing film

12: antifouling baffle

121: antifouling baffle

122: side wall

123: space of

124: shielding laminate

125: through hole

126: accommodation hole

127: protective sleeve

128: second fastener

13: protective cover

131: positioning concave part

2: magnetic rod sleeve

21: joint part

211: clamping piece

212: protruding piece

22: pipe body

23: end portion

3: magnetic rod sleeve connecting piece

31: engagement portion

32: channel

4: magnetic bar

H1: first height of

H2: second height

And H3: third height

D: distance of

Detailed Description

Some exemplary embodiments that exhibit the features and advantages of the present disclosure are described in detail in the following description. It will be understood that the utility model is capable of various modifications in various aspects, all without departing from the scope of the utility model, and that the description and illustrations herein are intended to be by way of illustration only and not to be construed as limiting the utility model.

Please refer to fig. 1A-1C, fig. 2A-2B, fig. 3, fig. 4, and fig. 5. Fig. 1A shows a schematic view of a nucleic acid extraction tray assembly according to an embodiment of the present disclosure, fig. 1B shows an exploded schematic view of a nucleic acid extraction tray assembly according to an embodiment of the present disclosure, fig. 1C shows a cross-sectional view of a nucleic acid extraction tray assembly according to an embodiment of the present disclosure, fig. 2A shows a top view of an extraction reagent well tray according to an embodiment of the present disclosure, fig. 2B shows a schematic view of an extraction reagent well tray according to another embodiment of the present disclosure, fig. 3 shows a top view of an anti-fouling shield according to another embodiment of the present disclosure, fig. 4 shows a schematic view of a nucleic acid extraction tray assembly according to another embodiment of the present disclosure, and fig. 5 shows a schematic view of a combination pattern of a magnetic bar sleeve, a magnetic bar sleeve connector, and a magnetic bar according to an embodiment of the present disclosure. The nucleic acid extraction disk assembly 1 includes an extraction reagent well plate 11 and an anti-fouling shield 12. The extraction reagent well plate 11 is a main container for performing nucleic acid extraction, and comprises a through groove 111 and a plurality of well groove groups 112, wherein the through groove 111 is a space for presetting the magnetic rod sleeve 2, and extraction reagents required for performing nucleic acid extraction are respectively pre-contained in the plurality of well groove groups 112, and each well groove group 112 comprises a plurality of well grooves 113 which are arranged in a straight line and respectively contain reagents required for performing nucleic acid extraction. For example, if the nucleic acid extraction operation requires 5 steps, the plurality of wells 113 in each well group 112 are further subdivided into five wells 113a, 113b, 113c, 113d, and 113e to accommodate reagents required for each step, such as lysate, washing solution, eluent, etc., and one well may be reserved to accommodate magnetic beads. Here, each well group 112 is used for a nucleic acid extraction process of one sample, so the number of well groups 112 provided on the extraction reagent well plate 11 is the number of samples on the extraction reagent well plate 11 that can be subjected to the nucleic acid extraction process simultaneously, and correspondingly, the automatic nucleic acid extraction device is also provided with an equal number of magnetic bars 4 to perform all the nucleic acid extraction operations on the same extraction reagent well plate 11 in batches. The number of the wells 113 of the extraction reagent well plate 11 may be varied according to the actual requirement, for example, 24 wells, 48 wells, 96 wells, etc., which is not limited by the embodiment of the extraction reagent well plate 11 in which only 96 wells are drawn in the drawings. In addition, since the reagents used for nucleic acid extraction are reagents which are conventional in the market, the reagents in the wells 113 can be preset therein when shipped from the factory, and the sealing film is used for sealing to ensure that the reagents are not mixed with each other, so that the labor and time for filling the reagents can be reduced, and the possible occurrence of reagent filling errors can be avoided. In one embodiment, as shown in FIG. 2B, after the reagent is filled into the extraction reagent well plate 11, the sealing film 116 is first used to seal the extraction reagent well plate to avoid leakage of the reagent, and the sealing film 116 is then torn off when the extraction reagent well plate is to be put on-machine.

The anti-fouling baffle 12 is disposed above the extraction reagent well plate 11, and the anti-fouling baffle 12 and the extraction reagent well plate 11 can be combined by, but not limited to, fastening. For example, the extraction reagent well plate 11 and the anti-fouling baffle 12 are respectively provided with a plurality of corresponding first fasteners 114 and a plurality of corresponding second fasteners 128, which can be fastened to each other, so as to arrange and fix the anti-fouling baffle 12 above the extraction reagent well plate 11. In one embodiment, the first fastening member 114 and the second fastening member 128 can be corresponding recesses and hooks, but not limited thereto.

The anti-fouling baffle 12 includes a plurality of anti-fouling baffles 121 and a plurality of sidewalls 122 intersecting the anti-fouling baffles 121 to isolate a plurality of spaces 123 corresponding to the plurality of hole groove sets 112 in the anti-fouling baffle 12, wherein each anti-fouling baffle 121 is disposed between adjacent hole groove sets 112, for example, just corresponding to the walls of the groove connected between two adjacent hole groove sets 112, and then the plurality of sidewalls 122 are matched, so that a plurality of spaces 123 just corresponding to the plurality of hole groove sets 112 can be enclosed by every two adjacent anti-fouling baffles 121 and the sidewalls 122. In other words, when the anti-fouling baffle 12 is fastened above the extraction reagent well plate 11, the anti-fouling baffles 121 extend upward corresponding to the walls of the well plates connected between the well plates 112, and more specifically, the operating space of each well plate 112 in the extraction reagent well plate 11 is provided by the anti-fouling baffles 121 and the side walls 122 of the anti-fouling baffle 12 extending upward to the spaces 123.

The anti-fouling barrier 12 also includes a plurality of shielding plates 124 disposed in each of the spaces 123. As shown in fig. 1C and 3, the shielding layer 124 is disposed in the space 123 in a direction substantially perpendicular to the anti-fouling baffle 121. By the arrangement of the shielding layer 124, the space 123 is further divided into a first space 1231 below and a second space 1232 above, and the cross section of the space 123 perpendicular to the anti-fouling baffle 121 and the side wall 122 is further reduced from the periphery to the through hole 125 due to the arrangement of the shielding layer 124. In a preferred embodiment, the through-hole 125 may have an oblong shape and span the aperture slots 113a-113e for the magnetic rod sleeve 2 to pass through and perform nucleic acid extraction operations.

The anti-fouling baffle 12 is further provided with a plurality of accommodating holes 126 corresponding to the through slots 111 of the extraction reagent hole tray 11, and each accommodating hole 126 is provided with a protecting sleeve 127 in an extending manner below each accommodating hole 126, so as to provide a function of protecting the magnetic rod sleeve 2, for example, to avoid the situation that the magnetic rod sleeve 2 is inadvertently polluted due to exposure when the anti-fouling baffle 12 is separated from the extraction reagent hole tray 11. In one embodiment, the length of the protective sleeve 127 is preferably such that the bar magnet sleeve 2 is completely shielded so that the bar magnet sleeve 2 is not exposed. More specifically, in this case, the magnetic rod sleeve 2 is preset in the anti-fouling blocking member 12, and moves along with the anti-fouling blocking member 12, and the positions of the magnetic rod sleeve 2 and the protection sleeve 127 corresponding to the extraction reagent hole tray 11 are through grooves 111 without separation, so that the efficiency of loading the magnetic rod sleeve 2 into the extraction reagent hole tray 11 can be effectively improved. In addition, since the accommodating hole 126 is designed corresponding to a single magnetic rod sleeve, the magnetic rod sleeve 2 is preferably designed in a single package, but is not limited thereto.

Furthermore, in order to provide protection during transportation and movement, as shown in FIG. 4, the nucleic acid extraction tray assembly 1 may further comprise a protection cover 13 disposed over the anti-fouling barrier 12 for isolating the nucleic acid extraction tray assembly 1 from external contact; in addition, the function of preventing the magnet bar cover 2 from falling off is provided when the anti-fouling shield 12 is temporarily removed from the extraction reagent well plate 11 in order to remove the sealing film 116 before the nucleic acid extraction operation is performed. The protecting cover 13 is provided with a plurality of positioning concave parts 131 which can be correspondingly clamped into the space 123 surrounded by the anti-fouling baffle plate 121 and the side wall 122 when the cover is arranged above the anti-fouling baffle plate 12, so as to strengthen the combination with the anti-fouling baffle plate 12.

Accordingly, the preparation flow before performing the nucleic acid extraction operation is: simultaneously, the anti-fouling baffle 12, the magnetic rod sleeve 2 and the protective cover 13 which are preset in the anti-fouling baffle 12 are removed, the sealing film 116 arranged on the extraction reagent pore disk 11 is exposed and removed, then the anti-fouling baffle 12, the magnetic rod sleeve 2 and the protective cover 13 are replaced, and then the protective cover 13 is removed, so that the preparation procedure is completed.

Then, the operation flow for performing nucleic acid extraction is as follows: after the magnetic rod sleeve connecting piece 3 arranged on the automatic nucleic acid extraction device is combined with the magnetic rod sleeve 2 arranged in the accommodating hole 126, the magnetic rod sleeve connecting piece 3 sequentially enters the hole groove 113a, the hole groove 113b, the hole groove 113c, the hole groove 113d and the hole groove 113e in the space 123, and the magnetic rod 4 enters the magnetic rod sleeve 2 through the magnetic rod sleeve connecting piece 3 to sequentially move the magnetic beads to the hole grooves, so that the magnetic rod sleeve connecting piece is sequentially mixed with each reagent, and the nucleic acid extraction step is completed. As can be seen from the above, the nucleic acid extraction operation is completed by sequentially moving the magnetic rod sleeve 2 and the magnetic rod 4 between each of the hole slots 113a-113e and contacting with the reagent, so that the magnetic rod sleeve 2, the magnetic rod sleeve connector 3, and the magnetic rod 4 repeat the processes of entering the hole slot, leaving the hole slot, and moving to the next hole slot, and at this time, since the magnetic rod sleeve 2 separated from each of the hole slots 113 is adhered with the sample and the reagent liquid, there is a high possibility that the liquid on the adjacent magnetic rod sleeve 2 will be splashed alternately during the movement, in which case the nucleic acid extraction operation of each hole slot group corresponds to one sample, the liquid cross splash between different hole slot groups represents the cross contamination between different samples, which is the situation that is desired to be avoided during the extraction process; furthermore, when the rod cover 2 is mixed in the hole 113 by up-down vibration to ensure extraction efficiency, aerosol may be generated, and cross infection may be generated by the air flow driven by the movement of the rod cover 2 and the rod 4, which is a condition that is desired to be avoided.

In this case, the anti-fouling barrier 12 is provided to prevent cross-contamination of different samples of different well sets 112 during nucleic acid extraction operations. First, since the contact of the magnetic rod cover 2 with the reagent liquid in the well 113 is mainly concentrated at the end 23 of the magnetic rod cover 2 near the bottom (as shown in fig. 6), when the magnetic rod cover 2 moves away from one well to another, for example, from the well 113a to the well 113b, the liquid-adhered portion is concentrated at the end 23, and accordingly, the arrangement of the anti-fouling baffle 121 helps to provide the function of separating the different well groups 112 when the magnetic rod cover 2 moves between the wells 113, and therefore, the range in which the plurality of anti-fouling baffles 121 extend upward from the top edge of the reagent-extracting well 11 is preferable to cover the end 23. In other words, as shown in fig. 1C, the first height H1 of the anti-fouling baffle 12 corresponding to the plurality of anti-fouling baffles 121 is preferably greater than the height of the end 23 of the magnetic rod sleeve 2, for example, the first height H1 may be between 7 mm and 10 mm. Furthermore, the shielding layer 124 is disposed to help reduce the risk of aerosol escaping from the space 123 along with the airflow during the oscillation, that is, when the aerosol escapes upward, the aerosol is blocked by the shielding layer 124 perpendicular to the direction of the anti-fouling baffle 121 and remains in the space 123, particularly the first space 1231, so as to further reduce the probability of cross-contamination between different samples. More specifically, in the anti-fouling barrier 12 of the present embodiment, the anti-fouling barrier 121 provides a horizontal separation and the shielding layer 124 provides a vertical shielding, so that each sample can perform nucleic acid extraction operation in the independent and non-interfering space 123 to the maximum extent under the combined action of the two.

In addition, in order to facilitate visual recognition of the labeling position 115 on the extraction reagent tray 11 for labeling the sample and the labeling text or bar code thereon, the side of the anti-fouling baffle 12 corresponding to the labeling position 115 is provided with a plurality of second height H2 lower than the anti-fouling baffles 121 to expose the labeling position 115 without being blocked by the anti-fouling baffle 12.

On the other hand, the magnetic rod sleeve 2 also has a novel design. Please refer to fig. 1C, fig. 5 and fig. 6. Fig. 6 shows a schematic diagram of a combination of a magnetic rod sleeve and a magnetic rod sleeve connector according to an embodiment of the present disclosure. The magnetic rod sleeve 2 comprises a joint portion 21, an end portion 23 opposite to the joint portion 21, and a pipe body 22 connecting the joint portion 21 and the end portion 23, wherein the joint portion 21 is used for being combined with the magnetic rod sleeve connector 3, and the end portion 23 is used for performing a nucleic acid extraction operation into the hole groove 113. The inner side of the joint portion 21 is provided with a plurality of engaging members 211, for example, a plurality of projections, for example, 3 projections, which are equidistantly distributed on the inner circumferential surface of the joint portion 21, and correspondingly, the magnetic rod sleeve connecting member 3 is provided with engaging portions 31, for example, annular recesses on the rod body of the magnetic rod sleeve connecting member 3, so as to engage corresponding engaging members 211, thereby achieving the combination of the two. And the magnetic rod sleeve connecting piece 3 used for being combined with the magnetic rod sleeve 2 is provided with a channel 32, when the magnetic rod sleeve connecting piece 3 is combined with the magnetic rod sleeve 2, the channel 32 is communicated with the inner space of the magnetic rod sleeve 2, and the magnetic rod 4 can pass through and extend into the magnetic rod sleeve 2.

The magnetic rod sleeve connecting piece 3 and the magnetic rod sleeve 2 are combined in such a way that the automatic nucleic acid extraction device moves the magnetic rod sleeve connecting piece 3 to be aligned above the magnetic rod sleeve 2 pre-arranged in the anti-fouling baffle 12, then moves downwards and enters the joint part 21, at this time, because the magnetic rod sleeve 2 is made of elastic materials, such as, but not limited to, plastic, the front end of the magnetic rod sleeve connecting piece 3 can enter the joint part 21 and continuously move downwards due to the elasticity of the materials until the clamping part 31 corresponds to the position of the clamping piece 211, and at this time, the clamping piece 211 is restored to the original position due to the elasticity of the materials and clamped into the clamping part 31, so that the combination between the two parts is completed. Wherein, the clamping part 31 is arranged on the magnetic rod sleeve connecting piece 3 in a surrounding way, so the combination between the magnetic rod sleeve connecting piece 3 and the magnetic rod sleeve 2 has no directivity. Under the structure, when the magnetic rod sleeve 2 oscillates up and down in the hole groove 113 to perform liquid mixing operation, the magnetic rod 4 is separated from the magnetic rod sleeve 2, and the automatic nucleic acid extraction device can control the action of the magnetic rod sleeve 2 through the magnetic rod sleeve connecting piece 3; when transferring the magnetic beads to different hole slots 113, the automatic nucleic acid extraction device passes the magnetic rod 4 arranged on the magnetic rod frame through the channel 32 of the magnetic rod sleeve connecting piece 3 and stretches into the end 23 of the magnetic rod sleeve 2, thereby magnetically attracting the magnetic beads and transferring the magnetic rod sleeve 2 and the magnetic beads adsorbed on the magnetic rod sleeve 2 to the next hole slot 113.

When the magnetic rod sleeve 2 is separated from the magnetic rod sleeve connector 3 after the nucleic acid extraction operation is completed, the magnetic rod 4 is only required to be further forced downwards to abut against the magnetic rod sleeve 2 by the elasticity of the material of the magnetic rod sleeve 2, so that the clamping part 211 is separated from the clamping part 31 by the force application, the separation operation between the magnetic rod sleeve 2 and the magnetic rod sleeve connector 3 is completed, and then the magnetic rod 4 is reset upwards. Therefore, through the design of the magnetic rod sleeve 2, the combination or separation of the magnetic rod sleeve connecting piece 3 and the magnetic rod 4 can be completed together through an automatic nucleic acid extraction device, and no human participation is needed.

Further, referring to fig. 1C, 3 and 6, the magnetic rod sleeve 2 is also provided with a special design corresponding to the anti-fouling baffle 12, based on the magnetic rod sleeve 2 being preset in the anti-fouling baffle 12, and the magnetic rod sleeve 2 passing through the anti-fouling baffle 12 to enter the extraction reagent hole tray 11 during the nucleic acid extraction operation. First, as shown in fig. 1C, the sum of the lengths of the tube body 22 and the end 23 of the magnetic rod sleeve 2 is approximately equal to the sum of the height of the hole groove 113 and the distance between the shielding plate 124 and the top edge of the extraction reagent hole tray 111 (i.e., the third height H3 of the first space 1231), that is, the position of the joint portion 21 is higher than the shielding plate 124. Furthermore, the diameter of the end 23 of the magnetic rod sleeve 2 and the diameter of the tube body 22 are smaller than the distance D between the two parallel sides of the through hole 125 surrounded by the shielding plate 124, and the diameter of the joint 21 is larger than the distance D, in other words, the tube body 22 and the end 23 can enter the first space 1231 and the hole slot 113 through the through hole 125, and the joint 21 cannot be left in the second space 1232 above the shielding plate 124 through the through hole 125. More specifically, the structure of the magnetic rod sleeve 2 has the following characteristics that one is that the length is further lengthened compared with the magnetic rod sleeve preset in the extraction reagent hole tray 11, and the other is that the structure has a smaller structural step with a larger upper end joint part 21, a lower end pipe body 22 and an end part 23. The advantage of this extended length and structural step is that the probability of contamination of the front end of the bar magnet sleeve connector 3 coupled to the coupling portion 21 can be further reduced. Because the magnetic rod sleeve connecting piece 3 is a member which is arranged on the automatic nucleic acid extraction device and needs to be reused, the magnetic rod sleeve connecting piece is not easy to clean, so that the magnetic rod sleeve connecting piece 3 is isolated outside the nucleic acid extraction operation space by maintaining the joint part 21 of the magnetic rod sleeve 2 above the shielding layer plate 124, the probability that the magnetic rod sleeve connecting piece 3 is polluted by the escaped aerosol can be reduced to the greatest extent, and the labor for cleaning the magnetic rod sleeve connecting piece 3 is reduced.

The structural step may also expose the joint portion 21 outside the accommodating hole 126 when the magnetic rod sleeve 2 is disposed in the accommodating hole 126, that is, the diameter of the joint portion 21 is larger than the diameter of the accommodating hole 126. This helps to align all the bar magnet sleeves 2 in a straight line and on the same plane, and improves the success rate of automatic taking of the bar magnet sleeves 2 by the automated nucleic acid extraction device.

In addition, the outer periphery of the joint portion 21 may also be provided with a plurality of protruding pieces 212, for example, a plurality of ribs parallel to the long axis direction of the bar magnet sleeve 2, to further ensure that the joint portion 21 can be maintained above the shielding layer plate 124, and that the bar magnet sleeve 2 can be more stably disposed on the plane where the accommodation hole 126 is formed.

In yet another aspect, the extraction reagent well plate 11 of the present disclosure further provides a structural design that helps to optimize the nucleic acid extraction process. Referring to fig. 1C, each well 113 in the extraction reagent well plate 11 includes an inlet 1131, a receiving space 1132, and a bottom portion 1133, wherein the bottom portion 1133 has a smaller aperture than other portions of the well 113, i.e. the cross section of the bottom portion 1133 in a long axis direction of the well 113 is smaller than that of other portions, and has a structure with a wide top and a narrow bottom, which is advantageous in that when the magnetic rod cover 2 oscillates up and down in the well 113 to cause the reagent liquid to disturb up and down in the well, the larger aperture/cross section helps to reduce splashing of the liquid, and thus reduces the probability of cross contamination between different well groups 112.

In some embodiments, the bottom portion 1133 may have different structural design variations, for example, the cross-sectional shape of the bottom portion 1133 may have different shapes such as a circle or a square, and/or the appearance of the bottom portion 1133 may be shaped like a U, a cone, a spindle, or the like, and by the cooperation between the two, the bottom portion 1133 may be changed to suit various requirements, for example, to emphasize the mixing effect, or to emphasize the cooperation with the shape of the magnetic rod sleeve 2.

In addition, as can be seen from FIG. 1C, the depth of the well 113e (i.e., the last well of the well group 112) is smaller than the depths of the other wells 113a-113d, and this design is because, first, the pipetting is performed by using a pipette with a small volume and a short length, such as a micropipette, after the nucleic acid extraction is completed, so that the smaller well depth contributes to the easier access of the liquid by the pipette and increases the convenience of operation. Furthermore, the last step of the nucleic acid extraction operation is to elute the extracted nucleic acid, and the liquid in the well 113e is used for analysis later, so the design of smaller Kong Caoshen degrees can reduce the splash range when the liquid is swabbed and splashed to adhere to the well wall, thereby being beneficial to improving the concentration of the redissolved nucleic acid liquid and facilitating the subsequent analysis operation.

In summary, the nucleic acid extraction tray assembly of the present disclosure achieves the effect of preventing cross contamination between different samples by providing the anti-fouling baffle on the extraction reagent tray, wherein the anti-fouling baffle provides the nucleic acid extraction operation space above the extraction reagent tray and corresponding to each sample of the extraction reagent tray, and also provides the shielding layer plate for preventing the aerosol from escaping in each space, so that the magnetic rod sleeve performs the up-and-down oscillation action and the liquid and/or aerosol splashes which may cause cross contamination and are generated by the operations such as moving between different wells during the nucleic acid extraction process by the magnetic bead method can be limited therein, thereby greatly reducing the probability of cross contamination.

In addition, the structure of the magnetic rod sleeve is also improved, so that the probability of pollution to the magnetic rod sleeve connecting piece can be reduced, the cleaning labor is reduced, and the combination and separation of the magnetic rod sleeve and the magnetic rod sleeve connecting piece can be completed by the automatic nucleic acid extraction device, so that the steps of manual participation are effectively reduced, and the efficiency of an automatic operation flow is improved.

It should be noted that the foregoing description of the preferred embodiments is provided for illustration only, and the scope of the present utility model is not limited to the embodiments described herein, but is defined by the claims. And that the present utility model may be modified in various ways by those skilled in the art without departing from the scope of the claims.

Claims (16)

1. A nucleic acid extraction tray assembly comprising:

an extraction reagent well plate having a plurality of well groups for performing nucleic acid extraction operations on a plurality of samples, wherein each well group comprises a plurality of well slots arranged in a straight line, and each well slot accommodates a reagent required for the nucleic acid extraction operations; and

an anti-fouling baffle, set up in this extraction reagent hole dish top, contain:

the accommodating holes are used for accommodating a magnetic rod sleeve respectively;

a plurality of anti-fouling baffles to separate a plurality of spaces above the extraction reagent well plate corresponding to the plurality of well groups, respectively; and

a plurality of shielding laminates which are correspondingly arranged in each space in the direction vertical to the plurality of antifouling baffles,

wherein an end portion of the magnetic rod cover to which the reagent is attached moves within the space during the nucleic acid extraction operation, and a joint portion of the magnetic rod cover with respect to the end portion moves above the shielding plate.

2. The nucleic acid extraction tray as claimed in claim 1, wherein the shielding plate is constructed such that a cross section of the space perpendicular to the anti-fouling plate is narrowed inward to form a through hole, and the through hole is for the end of the magnetic rod sleeve to pass through and move during the nucleic acid extraction operation.

3. The nucleic acid extraction tray assembly of claim 1, wherein the shielding plate divides the space into a first space located below and a second space located above, and the engaging portion of the magnetic rod sleeve moves in the second space during the nucleic acid extraction operation.

4. The nucleic acid extraction tray as claimed in claim 1, wherein the plurality of anti-fouling baffles are respectively corresponding to walls of the wells between each adjacent well group of the plurality of well groups to form the plurality of spaces.

5. The nucleic acid extraction tray of claim 1, wherein the anti-fouling barrier has a first height corresponding to the plurality of anti-fouling baffles, and the anti-fouling barrier has a second height on one side of the plurality of anti-fouling baffles, and the second height is less than the first height.

6. The nucleic acid extraction tray of claim 1, wherein the engaging portion of the magnetic rod sleeve is configured to be combined with a magnetic rod sleeve connecting piece, and a plurality of engaging members are disposed on an inner side of the engaging portion to engage with an engaging portion of the magnetic rod sleeve connecting piece.

7. The nucleic acid extraction disk assembly as claimed in claim 1, wherein a plurality of protruding members are provided on an outer side of the engaging portion of the magnetic rod sleeve.

8. The nucleic acid extraction tray of claim 1, wherein the magnetic rod sleeve further comprises a tube connecting the junction and the end, and the diameter of the junction is greater than the diameter of the tube and the diameter of the end.

9. The nucleic acid extraction tray assembly of claim 8, wherein the sum of the lengths of the tube and the end portion is equal to the sum of the height of the well and the distance between the shielding plate and the top edge of the extraction reagent well tray.

10. The nucleic acid extraction tray assembly of claim 1, wherein the anti-fouling shield further comprises a plurality of protection sleeves respectively disposed under each of the receiving holes to protect the magnetic rod sleeve received in the receiving hole.

11. The nucleic acid extraction tray of claim 10, wherein the extraction reagent well tray further comprises at least one through slot for correspondingly disposing the plurality of protective sleeves and the magnetic rod sleeve respectively disposed in the plurality of protective sleeves.

12. The nucleic acid extraction tray of claim 1, wherein the extraction reagent well tray has a plurality of first fasteners and the anti-fouling shield has a plurality of second fasteners, and the anti-fouling shield is secured over the extraction reagent well tray by the plurality of first fasteners and the plurality of second fasteners being engaged with each other.

13. The nucleic acid extraction disk assembly of claim 1, wherein the depth of the well for performing an elution step is less than the depth of the other wells of the plurality of wells.

14. The nucleic acid extraction tray as claimed in claim 1, wherein the well has an inlet, a receiving space, and a bottom portion, and a cross section of the bottom portion in a long axis direction of the well is smaller than a cross section of other portions of the well.

15. The nucleic acid extraction disk assembly of claim 1, further comprising a protective cover over the anti-fouling shield.

16. The nucleic acid extraction tray assembly of claim 1, further comprising at least one sealing membrane for sealing the plurality of wells of the extraction reagent well tray.