CN111796088A

CN111796088A - Multifunctional full-automatic colloidal gold detector

Info

Publication number: CN111796088A
Application number: CN202010688425.1A
Authority: CN
Inventors: 刘雄杰; 沈海东; 王慧; 李源; 朱晨阳; 赵立秀; 陈任远; 钱峰; 程娟
Original assignee: Jiangsu Bioperfectus Technologies Co ltd
Current assignee: Jiangsu Bioperfectus Technologies Co ltd
Priority date: 2020-07-16
Filing date: 2020-07-16
Publication date: 2020-10-20

Abstract

The invention provides a multifunctional full-automatic colloidal gold detector which comprises a feeding mechanism, an X-axis sampling mechanism, a TIP (TIP in TIP) conveying mechanism, an incubation mechanism, a detector mounting table and a control device, wherein a sample tube is mounted on the feeding mechanism, the feeding mechanism can add a diluent into the sample tube and uniformly mix the diluent to form a standby sample, a reagent card is placed on the incubation mechanism, and a TIP head is arranged on the TIP conveying mechanism; the X-axis sample adding mechanism is provided with a TIP needle rod, the X-axis sample adding mechanism can install a TIP head on the TIP needle rod, take out a sample from the sample tube through the TIP head and add the sample to the reagent card, and the reagent card added with the sample is conveyed to the incubation bin to finish incubation and then finish detection in the incubation mechanism.

Description

Multifunctional full-automatic colloidal gold detector

Technical Field

The invention relates to the technical field of medical equipment, in particular to a multifunctional full-automatic colloidal gold detector.

Background

The colloidal gold method is performed by chloroauric acid (HAuCl)₄) Under the action of reducing agent such as white phosphorus, ascorbic acid, sodium citrate, tannic acid, etc., gold particles with a certain size can be polymerized, and become a stable colloidal state due to electrostatic interaction to form a hydrophobic gel solution with negative charge, and become a stable colloidal state due to electrostatic interaction, so the gold colloid is called gold colloid. Colloidal gold is also an ideal immune marker in the immunoelectron microscopy technology.

Most of the existing colloidal gold detection equipment are automatic equipment, but the function is single, the whole detection process of the whole colloidal gold comprises the steps of dropwise adding a diluent into a sample, uniformly mixing the sample, adding the sample into a reagent card, incubating the reagent card, detecting the reagent card and the like, only the last step of detecting the reagent card is usually detected as an instrument, other steps are manually made, the detection result is influenced when the manual workload is huge in a comprehensive large hospital or in a season with high infectious diseases, the detection result is influenced when an operation error occurs in any previous link, the manual operation step is avoided as much as possible to achieve the accuracy of detection, the risk of the operation error can be reduced, and meanwhile, the workload of doctors is greatly reduced, and the working efficiency is improved.

Patent document CN209542625U discloses a colloidal gold detector, which comprises a sample introduction unit, an image collector, a screen display unit, a data processing unit and a control unit; the sample introduction unit comprises a colloidal gold detection card, a detection card fixer and a servo stepping motor; the detection card fixer is of a hollow frame structure, the colloidal gold detection card can be fixed on the detection card fixer, and a bulge is arranged at the bottom of the colloidal gold detection card; the servo stepping motor rotates and drives the colloidal gold detection card to move below the image collector, a detection clamping groove is also arranged below the image collector, a groove which is matched with the protrusion on the colloidal gold detection card in the direction is arranged on the detection clamping groove, a trigger sensor is arranged in the groove, and the sensor is connected with the data processing unit and the control unit; the control unit is connected with and controls the servo stepping motor and the image collector, but the design has no design on links such as sample dropwise adding diluent, sample mixing, sample adding to a reagent card, reagent card incubation and the like, and the detection efficiency and the detection quality can not be guaranteed in actual detection.

Disclosure of Invention

Aiming at the defects in the prior art, the invention aims to provide a multifunctional full-automatic colloidal gold detector.

The invention provides a multifunctional full-automatic colloidal gold detector which comprises a feeding mechanism, an X-axis sampling mechanism, a TIP (TIP in process) conveying mechanism, an incubation mechanism, a detector mounting table and a control device, wherein the feeding mechanism is arranged on the X-axis sampling mechanism;

the feeding mechanism, the X-axis sample adding mechanism, the TIP conveying mechanism and the incubation mechanism are all arranged on a detector mounting table;

the feeding mechanism comprises a sample rack, a sample tube is arranged on the sample rack, and the feeding mechanism can add diluent into the sample tube and uniformly mix the diluent to form a sample for standby under the control of the control device;

the incubation mechanism comprises a reagent card and an incubation bin;

the TIP transmission mechanism is provided with a TIP head;

the X-axis sample adding mechanism is provided with a TIP needle rod, under the control of the control device, the X-axis sample adding mechanism can install a TIP head on the TIP needle rod, take out a sample from the sample tube through the TIP head and add the sample to the reagent card, and transport the reagent card added with the sample to an incubation bin to finish detection in the incubation mechanism after incubation is finished.

Preferably, the feeding mechanism comprises a bearing frame, a first driving device, a second driving device, a liquid adding device, a squeezing device and a discharging device;

the loading plate is mounted on the loading frame and sequentially comprises a first material feeding position, a liquid feeding extrusion position and a discharging position along the length direction;

the first driving device and the discharging device are respectively arranged on a first material feeding position and a first material discharging position, and the liquid adding device and the extruding device are respectively arranged on a liquid adding extruding position;

the second driving device is installed on the bearing frame and is provided with a transmission carrier and a second motor, a first through hole is formed in the middle of the bearing plate along the length direction of the bearing plate, a bearing space is formed in the upper end of the transmission carrier and is installed on the bearing plate, the lower end of the transmission carrier penetrates through the first through hole to extend to the lower portion of the bearing plate and is in driving connection with the second motor, and the sample frame can be installed in the bearing space in a matched mode;

the first feeding position is connected with a sample rack storage area, and the sample rack placed in the sample rack storage area can be conveyed to the bearing space under the driving of the first driving device;

under the driving of the second motor, the transmission carrier can drive the sample rack to move from the first feeding position to the discharging position through the liquid adding extrusion position, and the sample rack moving to the discharging position can be pushed out to leave the discharging position under the driving of the discharging device;

the sample rack comprises a sample rack base and a sample tube bearing body;

the sample tube carrier is connected with the sample frame base through a plurality of connecting ribs;

the utility model discloses a sample frame, including sample frame base, sample pipe bearing carrier, mounting groove, the mounting groove of a plurality of indents is set up to the equidistance in proper order on the sample frame base, the equidistance has set up the space of placing of a plurality of cylindrics on the sample pipe bearing carrier in proper order, place space and mounting groove one-to-one from top to bottom, install in the mounting groove through placing the space when the sample pipe is placed, wherein, the one end setting of splice bar is between two adjacent mounting grooves, the other end setting of splice bar is between two adjacent spaces of placing, the width of splice bar is.

Preferably, the second driving device further comprises a first driving lead screw, a first bearing seat and a second bearing seat;

the utility model discloses a bearing plate, including bearing plate, first bearing frame, second bearing frame, transmission carrier, first drive lead screw, second drive lead screw, first bearing frame, second bearing frame, first drive lead screw, second motor and second drive lead screw, first bearing frame, second bearing frame and second motor drive are connected to the drive mode of first drive lead screw includes following any kind of structural style:

-using a synchronous belt drive;

-using a coupling for transmission;

-using a gear drive;

the first driving device comprises a first motor, a protection plate, a first belt wheel, a first synchronous belt, a second belt wheel, a transmission part and a driving push plate;

the driving push plate is arranged above the sample rack storage area, the second belt wheel is arranged on the side surface of the sample rack storage area, and the transmission part is arranged on the first synchronous belt and connected with the driving push plate;

one end of the first synchronous belt is sleeved on the second belt wheel, the other end of the first synchronous belt is sleeved on the first belt wheel, and the first belt wheel is in driving connection with the first motor;

when the first motor rotates, the first belt wheel can be driven to rotate and drive the first synchronous belt to move so as to drive the transmission part to drive the driving push plate to push the sample rack placed on the sample rack storage area into the bearing space;

the protection plate is characterized in that the first motor and the first belt wheel are respectively arranged on two sides of the protection plate, the transmission part and the driving push plate are respectively arranged on two sides of the protection plate, the protection plate is provided with a second through hole, and the transmission part is connected with the driving push plate penetrating through the second through hole.

Preferably, the liquid adding device comprises a liquid adding motor and a sample adding assembly, a sample clamping arm, a peristaltic pump, a stepping motor and a sample adding needle are arranged on the sample adding assembly, and the peristaltic pump is in driving connection with the stepping motor;

when the sample frame moves to the liquid adding extrusion position, the sample clamping arm clamping the sample adding needle can move to the upper part of the sample frame under the driving of the liquid adding motor and is connected with the sample adding needle through the peristaltic pump to complete liquid adding to the sample tube arranged on the bearing frame;

the extrusion device comprises a clamping head, a fixing plate and a spring;

the clamping head and the fixing plate are both arranged on the liquid adding extrusion position;

the number of the clamping heads is four, two of the clamping heads are arranged on one side of the first through hole, the other two of the clamping heads are arranged on the other side of the first through hole, the other two of the clamping heads are connected with the fixing plate, and springs are arranged in the other two of the clamping heads;

the clamping heads on the two sides correspond to each other in pairs, a narrow channel is formed between every two corresponding clamping heads, and the width of the narrow channel is smaller than the diameter of the sample tube;

the discharging device comprises a discharging motor, a discharging push rod, a second driving screw rod, a discharging base and a discharging guide rod;

the discharging base is installed on a discharging position, the discharging motor is installed on the discharging base, two discharging through holes are respectively formed in the discharging base and are respectively arranged on two sides of the discharging motor, one ends of the two discharging guide rods are respectively installed on the discharging push rod, and the other ends of the two discharging guide rods are respectively installed in the two discharging through holes and can slide relative to the discharging through holes;

one end of the second driving screw rod is installed on the discharging push rod, the other end of the second driving screw rod is connected with the discharging motor, when the discharging motor runs, the second driving screw rod can be driven to drive the discharging push rod to move along the length direction of the second driving screw rod, and meanwhile, the two discharging guide rods move along with the discharging push rod and slide in the two discharging through holes.

Preferably, the incubation mechanism further comprises a reagent card transport mechanism and a stage;

the object stage is provided with a supporting plate, the supporting plate sequentially comprises a discarding position, a second feeding position, a detection position, a sampling position and an incubation position towards the direction close to the incubation bin, and the incubation position extends into the incubation bin;

the reagent card conveying mechanism comprises a conveying hook which is arranged on the supporting plate and can move between the discarding position and the incubation position under the driving of the reagent card conveying mechanism;

the reagent card is placed on the lifting plate, the lifting plate can move between a first position and a second position under the driving of the first power assembly, and the first power assembly is driven by a motor or an air cylinder;

when the lifting plate drives the reagent card to move to the first position, the conveying hook moves to the second feeding position under the driving of the reagent card conveying mechanism, the lifting plate moves to the second position under the driving of the first power assembly at the moment, the reagent card enters the working range of the conveying hook, and the conveying hook can drive the reagent card to sequentially pass through the detection position, the sampling position and enter the incubation position;

the device comprises an incubation bin, wherein the incubation bin is internally provided with two incubation sub-bins, the two incubation sub-bins are respectively arranged on two sides of the interior of the incubation bin at intervals along the width direction of a supporting plate, the incubation sub-bins comprise a plurality of incubation layers, the incubation layers are arranged in the incubation sub bins at equal intervals along the vertical direction, and a conveying hook entering the incubation positions can drive a reagent card which is already incubated on another incubation layer to move to a detection position and then finally convey the detected reagent card to a discarding position to be discarded;

the incubation bin comprises an incubation bin housing and an incubation motor;

the incubation bin is mounted inside the incubation bin housing;

a first sliding rail is arranged on the inner wall of the incubation bin shell along the height direction, a sliding block is arranged on the incubation bin, and the sliding block is installed on the first sliding rail in a matching mode;

the incubation motor is arranged above the incubation bin shell and provided with an incubation driving rod;

the incubation driving rod penetrates through the top of the incubation bin shell, extends into the incubation bin shell and is connected with the incubation bin, an incubation position through hole is formed in the incubation position, and when the incubation motor operates, the incubation driving rod can be driven to move upwards or downwards so as to drive the incubation bin to move upwards or downwards through the incubation position through hole under the guidance of the first sliding rail;

a darkroom and a camera are arranged on the detection position;

the camera is arranged on the supporting plate through the darkroom;

the darkroom is arranged on the support plate, and a third gap is formed between the darkroom and the support plate; be provided with on the darkroom and shoot the through-hole, the camera can shoot the reagent card of placing on the detection position through shooting the through-hole.

Preferably, the reagent card transfer mechanism further comprises a transfer guide rod, a transmission carrier and a second power assembly;

the object stage also comprises a third supporting leg and a fourth supporting leg, and the third supporting leg and the fourth supporting leg are respectively arranged at two ends of the lifting plate;

two ends of the transmission guide rod are respectively arranged on the third supporting leg and the fourth supporting leg and are arranged below the lifting plate;

the transmission carrier is provided with a transmission carrier through hole, the lower end of the transmission carrier is sleeved on the transmission guide rod through the transmission carrier through hole and can slide relative to the transmission guide rod, and the upper end of the transmission carrier is connected with the transmission hook;

the second power assembly can drive the transmission carrier to slide along the direction of the transmission guide rod and simultaneously drive the transmission hook to slide on the lifting plate;

the second power assembly comprises a transmission motor, a third synchronous belt, a first driving synchronous wheel, a fourth synchronous belt, a driven synchronous wheel, an upper computer and a second driving synchronous wheel;

one end of the third synchronous belt is connected with the conveying motor, the other end of the third synchronous belt is sleeved on the first driving synchronous wheel, one end of the fourth synchronous belt is sleeved on the second driving synchronous wheel, the other end of the fourth synchronous belt is sleeved on the driven synchronous wheel, and one side of the fourth synchronous belt is connected with the transmission carrier;

the first driving synchronizing wheel and the second driving synchronizing wheel are respectively arranged at two ends of the connecting shaft, and the connecting shaft is rotatably arranged on the support;

when the conveying motor rotates, the third synchronous belt can be driven to drive the first driving synchronous wheel to rotate, then the second driving synchronous wheel can be driven to synchronously rotate and drive the other end of the fourth synchronous belt to move around the driven synchronous wheel, the transmission carrier moves along with the fourth synchronous belt at the moment, an encoder is arranged on the conveying motor, and the encoder is electrically connected with an upper computer.

Preferably, a rectangular hole is formed in the middle of the supporting plate along the length direction, and the upper end of the transmission carrier penetrates through the rectangular hole to be connected with the conveying hook and can drive the conveying hook to move along the direction of the rectangular hole;

the conveying hook is of an I-shaped structure; the left side and the right side of the conveying hook form a first accommodating space and a second accommodating space respectively, and when two reagent cards are placed into the first accommodating space and the second accommodating space respectively, the conveying hook can push the reagent cards to move between the discarding position and the incubation position when moving;

the discarding position is provided with two discarding through holes which respectively correspond to the first accommodating space and the second accommodating space in the vertical direction.

Preferably, the X-axis sample loading mechanism comprises a sample loading supporting shell and a sample loading moving assembly;

a second sliding rail is arranged on the sample adding supporting shell;

the sample adding movement assembly comprises an X-direction movement bracket, an X-direction motor, a fourth synchronous belt, an X-direction driving wheel and an X-direction driven wheel;

the second slide rail, the X-direction motor, the X-direction driving wheel and the X-direction driven wheel are all arranged on the sample adding supporting shell;

two ends of the fourth synchronous belt are respectively sleeved on the X-direction driving wheel and the X-direction driven wheel;

the X-direction moving support is mounted on the second slide rail and can slide on the second slide rail, and the X-direction moving support is connected with the fourth synchronous belt;

x is just installed respectively in the both sides of application of sample support housing to motor, X to the action wheel X can order about X to the action wheel and rotate and order about fourth hold-in range and drive X to the direction slip of motion support along second slide rail length to the motor.

Preferably, the sample adding movement assembly comprises a plunger pump, a Z-direction motor, a third driving screw rod, a third slide rail and a Z-direction movement bracket;

the Z-direction motor and the third slide rail are both arranged on the X-direction moving support, and the Z-direction moving support is arranged on the third slide rail and can slide along the third slide rail;

one end of the third driving screw rod is connected with the Z-direction motor, the other end of the third driving screw rod is connected with the Z-direction moving support, and when the Z-direction motor rotates, the third driving screw rod rotates to drive the Z-direction moving support to vertically move upwards or downwards along the third sliding rail;

the TIP needle bar is arranged on the Z-direction movement bracket, and the plunger pump is connected with the TIP needle bar through a pipeline.

Preferably, the TIP conveying mechanism comprises a TIP cassette, a TIP cassette conveying motor, a TIP cassette conveying driving wheel, a TIP cassette conveying driven wheel, a TIP cassette conveying support platform, a TIP cassette conveying linkage frame and a TIP cassette synchronous belt;

the TIP box conveying linkage frame is arranged below the TIP box supporting plate, two ends of the TIP box conveying linkage frame extend outwards and upwards and are respectively connected with two ends of the TIP box sliding plate;

the TIP box transmission linkage frame is connected with the TIP box synchronous belt;

the TIP box conveying motor, the TIP box conveying driving wheel and the TIP box conveying driven wheel are all arranged on the TIP box conveying supporting table, and two ends of the TIP box synchronous belt are respectively sleeved on the TIP box conveying driving wheel and the TIP box conveying driven wheel;

when the TIP box conveying motor rotates, the TIP box conveying driving wheel can be driven to rotate and drive the TIP box synchronous belt to move, and then the TIP box conveying linkage frame is driven to be in driving connection with the TIP box sliding plate, and the TIP box moves on the TIP box supporting plate.

Compared with the prior art, the invention has the following beneficial effects:

1. the invention carries out automatic design aiming at sample pretreatment, sample adding, reagent card incubation and reagent card detection, integrates into a full-automatic device, a doctor only needs to put the sample and the reagent card into the device for subsequent full-automatic treatment, thereby greatly reducing the operation error and the workload of the doctor, automatically feeding the sample, automatically adding diluent, automatically mixing the sample, automatically discharging the sample in a test tube rack, improving the detection accuracy and greatly improving the detection efficiency.

2. The TIP conveying mechanism can automatically convey the TIP box to the designated position, so that the X-axis sample adding mechanism can conveniently take the TIP head, and the automation level of the device is improved.

3. The incubation mechanism can automatically grab the reagent card, the reagent card enters the sample adding position to be dropwise added with the sample, then the sample is incubated in the incubation bin, the incubation is finished, the detection mechanism is used for photographing the detection result, and finally the detected reagent card is discarded, so that the detection efficiency is greatly improved.

4. The X-axis sample adding mechanism can realize transverse movement in the X direction and vertical up-and-down movement in the Z direction to realize the installation of the TIP head and the action of sucking a sample, then dropwise add a sample solution into the detection reagent card for incubation, and finally discard the TIP head.

5. According to the invention, the mixing operation is realized by the mixing mechanism, the accuracy and consistency of sample pretreatment are ensured, the diluent is added by adopting the peristaltic pump to be matched with the stepping motor, the liquid adding precision is improved, the mixing quality and precision are improved, the actual requirements are met, the practicability is strong, and the market promotion prospect is great.

6. The invention adopts extrusion and blending to mix evenly, so that the sample tube is deformed through two narrow zones, the cotton swab is extruded by the deformed tube wall to achieve the function similar to that of pinching by hand, and the extrusion position is fixed and the extrusion equipment is not changed, thereby ensuring that the extrusion effect can be consistent every time, and greatly improving the quality of mixing evenly.

7. The invention designs the first material feeding position, the liquid adding extrusion position and the material discharging position and combines a plurality of motors to continuously carry out the whole blending operation, thereby not only ensuring the blending quality, but also improving the efficiency.

Drawings

Other features, objects and advantages of the invention will become more apparent upon reading of the detailed description of non-limiting embodiments with reference to the following drawings:

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic structural view of an X-axis sample application mechanism;

FIG. 3 is a schematic diagram of the TIP transport mechanism;

FIG. 4 is a schematic structural view of a feeding mechanism;

FIG. 5 is a schematic view of a structure of a sample rack;

FIG. 6 is a schematic structural diagram of a first driving device;

FIG. 7 is a schematic structural diagram of a second driving device;

FIG. 8 is a schematic view of the liquid adding device;

FIG. 9 is a schematic view of the pressing apparatus;

FIG. 10 is a schematic structural view of a discharging device;

FIG. 11 is a schematic view of the structure of the incubation mechanism;

FIG. 12 is a schematic view of the discard and feed locations;

FIG. 13 is a schematic diagram of the structure of an incubation silo;

FIG. 14 is a schematic diagram of the structure of the reagent card transport mechanism.

The figures show that:

carriage 1 sample tube carrier 33 incubation motor 69

Incubation chamber 70 for carrying plate 2 at first feed position 34

Sample rack storage area 3 liquid adding extrusion position 35 incubation driving rod 71

Sample rack 4 discharge level 36 TIP head 72

Stage 41 TIP needle bar 73 of transmission carrier 5

First motor 6 abandons position 42 application of sample and supports casing 74

Second motor 7 second feed position 43 plunger pump 75

First drive screw 8 detection position 44 second slide rail 76

45Z-direction motor 77 for sample loading position of first bearing block 9

Second bearing 10 incubation site 46X motion mount 78

Third drive screw 79 for the first support leg 11 transfer hook 47

Third slide rail 80 of second supporting leg 12 lifting plate 48

First power assembly 49X-direction motor 81 of protective plate 13

First pulley 14 support plate 50 fourth timing belt 82

The first synchronous belt 15 is provided with a reagent card 51X direction driving wheel 83

The second pulley 16 carries the motor 52X to the driven pulley 84

Third synchronous belt 53Z-direction movement bracket 85 of transmission piece 17

First driving synchronizing wheel 54 TIP box 86 of drive pusher plate 18

Fourth timing belt 55 TIP cassette transport motor 87 of priming motor 19

First through hole 20 driven synchronizing wheel 56 TIP cassette transfer capstan 88

Second through hole 21 transmission guide rod 57 TIP cassette transmission follower 89

The clamping head 22 drives the carrier 58 TIP cassette transport support table 90

Fixing plate 23 support 59 TIP box transfer linkage 91

Sample addition needle 24 incubation feed bin 60 TIP cassette timing belt 93

Second driving synchronizing wheel 61 TIP box sliding plate 92 of discharging motor 25

Third support leg 62 feed mechanism 100 of discharging push rod 26

Second drive lead screw 27, fourth supporting leg 63 and X-axis sample adding mechanism 200

First limiting plate 64 TIP conveying mechanism 300 of discharging base 28

Second limiting plate 65 incubation mechanism 400 of ejection of compact guide bar 29

Sample clamping arm 30 darkroom 66 detector mounting table 500

Sample tube 31 camera 67

Sample rack base 32 incubation bin housing 68

Detailed Description

The present invention will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any way. It should be noted that it would be obvious to those skilled in the art that various changes and modifications can be made without departing from the spirit of the invention. All falling within the scope of the present invention.

The invention provides a multifunctional full-automatic colloidal gold detector, which comprises a feeding mechanism 100, an X-axis sample adding mechanism 200, a TIP (TIP in phase) conveying mechanism 300, an incubation mechanism 400, a detector mounting table 500 and a control device, wherein the feeding mechanism 100, the X-axis sample adding mechanism 200, the TIP conveying mechanism 300 and the incubation mechanism 400 are all mounted on the detector mounting table 500, a sample rack 4 is mounted on the feeding mechanism 100, a sample tube 31 is mounted on the sample rack 4, under the control of the control device, the feeding mechanism 100 can add a diluent into the sample tube 31 and uniformly mix the diluent to form a standby sample, the incubation mechanism 400 comprises an incubation bin 60, a reagent card 51 is placed on the incubation mechanism 400, and a TIP head 72 is arranged on the TIP conveying mechanism 300; the X-axis sample adding mechanism 200 is provided with a TIP needle rod 73, under the control of the control device, the X-axis sample adding mechanism 200 can mount a TIP head 72 on the TIP needle rod 73 and can take out a sample from the sample tube 31 through the TIP head 72 to be added to the reagent card 51, and under the control of the control device, the reagent card 51 added with the sample is conveyed to the incubation bin 60, and after incubation is completed, detection is completed in the incubation mechanism 400.

Specifically, as shown in fig. 1 and 4, the feeding mechanism 100 includes a bearing frame 1, a first driving device, a second driving device, a liquid adding device, an extruding device and a discharging device, the bearing frame 1 is provided with a bearing plate 2, the bearing plate 2 sequentially includes a first liquid feeding position 34, a liquid adding extruding position 35 and a discharging position 36 along a length direction, the first driving device and the discharging device are respectively installed on the first liquid feeding position 34 and the discharging position 36, the liquid adding device and the extruding device are respectively installed on the liquid adding extruding position 35, the second driving device is installed on the bearing frame 1 and the second driving device is provided with a transmission carrier 5 and a second motor 7, a first through hole 20 is provided in the middle of the bearing plate 2 along the length direction of the bearing plate 2, the upper end of the transmission carrier 5 is provided with a bearing space and is installed on the bearing plate 2, the lower end of the transmission carrier 5 passes through the first through hole 20, extends to the lower part of the bearing plate 2 and is in driving connection with the second motor 7, wherein the sample rack 4 can be installed in the bearing space in a matching way, and the sample tube 31 is installed on the sample rack 4; the first feeding position 34 is connected with a sample rack storage area 3, and a sample rack 4 placed in the sample rack storage area 3 can enter the bearing space under the driving of a first driving device; under the drive of the second motor 7, the transmission carrier 5 can drive the sample rack 4 to move from the first feeding position 34 to the discharging position 36 through the filling extrusion position 35, and the sample rack 4 moved to the discharging position 36 can leave the discharging position 36 under the drive of the discharging device. The liquid adding device can replace the work of adding the diluent by a doctor, the extruding device replaces manual mixing, only the feeding part and the discharging part are used for manually adding and taking away the sample rack 4 for the doctor, and the working efficiency is greatly improved.

Further, as shown in fig. 1 and 6, in practical application, the sample rack storage area 3 can be used for placing a plurality of sample racks 4 side by side at a time, and the driving push plate 18 can move by the width of only one sample rack 4 at a time under the driving of the first motor 6 when the blending mechanism moves, so that one sample rack 4 is pushed into the bearing space at a time, thereby improving the efficiency of blending operation.

Specifically, as shown in fig. 1, 4 and 7, the bearing frame 1 includes a first supporting leg 11 and a second supporting leg 12, the first supporting leg 11 and the second supporting leg 12 are respectively installed at two ends of the bearing plate 2, the first supporting leg 11 and the second supporting leg 12 play a supporting role, and meanwhile, the area enclosed by the first supporting leg 11 and the second supporting leg 12 and the bearing plate 2 provides a placing space for the second driving device.

Further, as shown in fig. 1, 4 and 7, the second driving device further includes a first driving lead screw 8, a first bearing seat 9 and a second bearing seat 10; the utility model discloses a bearing frame, including bearing frame 2, first bearing frame 9, second bearing frame 10, the lower extreme of transmission carrier 5 is provided with the internal thread hole, be provided with on the first drive lead screw 8 with internal thread hole assorted external screw thread, the one end of first drive lead screw 8 is installed on first bearing frame 9, the other end of first drive lead screw 8 passes internal thread hole, second bearing frame 10 in proper order and is connected with the drive of second motor 7, and the external screw thread setting of first drive lead screw 8 is in the middle part, the position of bearing frame is connected at both ends respectively with bearing inner race interference fit.

Specifically, in a preferred example, as shown in fig. 7, the second motor 7 and the first driving screw 8 adopt a synchronous belt transmission, for example, a matching manner of a second synchronous belt and a pulley is adopted to realize transmission; in a variation, the second motor 7 and the first driving screw 8 are driven by a coupling, for example, the second motor 7 is connected with a matched speed reducer and then connected with the first driving screw 8 by the coupling to realize driving; in another variation, the second motor 7 and the first driving screw 8 are in gear transmission, for example, the second motor 7 is connected with a gear set, a gear engaged with the gear set is arranged on the first driving screw 8, and the transmission is realized through the gear transmission connection mode.

Specifically, as shown in fig. 1, 4 and 6, the first driving device includes a first motor 6, a first pulley 14, a first synchronous belt 15, a second pulley 16, a transmission member 17 and a driving push plate 18; the driving push plate 18 is arranged above the sample rack storage area 3, the second belt wheel 16 is arranged on the side surface of the sample rack storage area 3, and the transmission piece 17 is arranged on the first synchronous belt 15 and connected with the driving push plate 18; one end of the first synchronous belt 15 is sleeved on the second belt wheel 16, the other end of the first synchronous belt 15 is sleeved on the first belt wheel 14, and the first belt wheel 14 is in driving connection with the first motor 6; when the first motor 6 rotates, the first belt wheel 14 can be driven to rotate, and because the first synchronous belt 15 is in a tightening state between the first belt wheel 14 and the second belt wheel 16, the first belt wheel 14 rotates to drive the first synchronous belt 15 to make an oblong track motion, and then the transmission member 17 can be driven to drive the driving push plate 18 to push the sample rack 4 placed on the sample rack storage area 3 into the bearing space.

Specifically, as shown in fig. 1, 4, and 6, the first driving device further includes a protection plate 13, wherein the first motor 6 and the first pulley 14 are respectively installed on two sides of the protection plate 13, the transmission member 17 and the driving push plate 18 are respectively installed on two sides of the protection plate 13, the protection plate 13 is provided with a second through hole 21, and the transmission member 17 is connected to the driving push plate 18 passing through the second through hole 21.

Specifically, as shown in fig. 1, fig. 4, and fig. 8, the liquid adding device includes liquid adding motor 19 and application of sample subassembly, the application of sample subassembly includes clamp appearance arm 30, peristaltic pump, step motor and application of sample needle 24, the peristaltic pump is connected with the step motor drive, liquid adding motor 19 can order about clamp appearance arm 30 along the axial rotation of liquid adding motor 19, application of sample needle 24 is by the centre gripping of clamp appearance arm 30, when sample frame 4 moved to liquid adding extrusion position 35, the clamp appearance arm 30 that the centre gripping has application of sample needle 24 can move to the top of sample frame 4 and connect application of sample needle 24 through the peristaltic pump and accomplish the liquid adding to installing in the sample pipe 31 on bearing frame 1 under the order to add liquid motor 19. The invention adopts the peristaltic pump for adding the diluent, the liquid adding amount accurately controls the number of turns of the pump rotation by the stepping motor, and the peristaltic pump is characterized in that the liquid amount of each turn is constant, so the liquid adding accuracy can be controlled to meet the requirement by controlling the number of turns

Specifically, as shown in fig. 1, 4 and 9, the pressing device includes a clamping head 22, a fixing plate 23 and a spring; the clamping head 22 and the fixing plate 23 are both arranged on the liquid adding extrusion position 35; the number of the clamping heads 22 is four, and a flexible structure is adopted, wherein two clamping heads 22 are installed on one side of the first through hole 20, the other two clamping heads 22 are installed on the other side of the first through hole 20, the other two clamping heads 22 are connected with the fixing plate 23, and springs are installed inside the other two clamping heads 22; wherein two liang of correspondences of the tight head 22 of clamp of both sides and per two corresponding tight heads 22 of clamp form narrow passageway between, the width of narrow passageway is less than the diameter of sample pipe 31 and the width of narrow passageway can be adjusted according to actual conditions, because the sample pipe 31 outer wall is softer, can cause the extrusion deformation of sample pipe 31 when the sample pipe passes through narrow passageway, by the cotton swab in the 31 wall extrusion pipe of sample pipe, reach the effect that the hand was held between the fingers, wherein have the spring to make the suitable elasticity of the tight head of clamp in the tight head 22 of one side clamp, guarantee that sample frame 4 can pass through narrow passageway smoothly.

Specifically, as shown in fig. 1, 4, and 10, the discharging device includes a discharging motor 25, a discharging push rod 26, a second driving screw 27, a discharging base 28, and discharging guide rods 29, wherein the discharging base 28 is installed on a discharging position 36, the discharging motor 25 is installed on the discharging base 28, two discharging through holes are respectively formed in the discharging base 28 and are respectively arranged on two sides of the discharging motor 25, the number of the discharging guide rods 29 is two, one end of each of the two discharging guide rods 29 is respectively installed on the discharging push rod 26, and the other end of each of the two discharging guide rods 29 is respectively installed in the two discharging through holes and can slide relative to the discharging through holes; one end of the second driving screw 27 is mounted on the discharging push rod 26, the other end of the second driving screw 27 is connected with the discharging motor 25, when the discharging motor 25 operates, the second driving screw 27 can be driven to drive the discharging push rod 26 to move along the length direction of the second driving screw 27, and meanwhile, the two discharging guide rods 29 move along with the discharging push rod 26 and slide in the two discharging through holes. The discharging position 36 directly drives the discharging push rod 26 to push the sample rack into the sample discharging position by the discharging motor 25.

In particular, as shown in fig. 1, 4 and 5, the sample rack 4 comprises a sample rack base 32 and a sample tube carrier 33, the sample tube carrier 33 is connected with the sample frame base 32 through a plurality of connecting ribs, a plurality of inwards concave mounting grooves are sequentially arranged on the sample frame base 32 at equal intervals, a plurality of cylindrical placing spaces are sequentially arranged on the sample tube carrier 33 at equal intervals, the placing spaces correspond to the mounting grooves one by one, the sample tubes 31 are arranged in the mounting grooves through the placing spaces when placed, wherein one end of the connecting rib is arranged between two adjacent mounting grooves, the other end of the connecting rib is arranged between two adjacent placing spaces, the width of the connecting rib is smaller than the diameter of the sample tube 31, so that the clamping heads 22 on both sides can be pressed against both sides of the sample tube 31 without being blocked by the connecting ribs.

Specifically, as shown in fig. 1 and 11, the incubation mechanism 400 includes a reagent card conveying mechanism, a stage 41 and an incubation bin 60, a support plate 50 is disposed on the stage 41, and the support plate 50 sequentially includes a disposal position 42, a second feeding position 43, a detection position 44, a loading position 45 and an incubation position 46 in a direction close to the incubation bin 60, wherein the incubation position 46 extends to the inside of the incubation bin 60; the reagent card conveying mechanism comprises a conveying hook 47, the conveying hook 47 is mounted on a supporting plate 50 and can move between a discarding position 42 and an incubation position 46 under the driving of the reagent card conveying mechanism, according to the detection sequence of the reagent cards 51, the reagent cards 51 are taken out from the second feeding position 43 to enter a loading position 45, a detection sample is dripped on the loading position 45 and then enters the incubation position 46, the detection sample enters the detection position 44 for photographing detection after being incubated for 10 minutes at a rated temperature, and finally the detection sample enters the discarding position 42 to be discarded.

Further, as shown in fig. 1, 11 and 12, a lifting plate 48 and a first power assembly 49 are disposed on the second feeding position 43, a reagent card 51 is placed on the lifting plate 48 by a first mechanical arm, the number of the lifting plates 48 is two, two lifting plates 48 are disposed on two sides of the conveying hook 47, the lifting plate 48 is driven by the first power assembly 49 to move between a first position and a second position, when the lifting plate 48 drives the reagent card 51 to move to the first position, the conveying hook 47 is driven by the reagent card conveying mechanism to move to the second feeding position 43, the lifting plate 48 is driven by the first power assembly 49 to move to the second position, the reagent card 51 enters the working range of the conveying hook 47, the conveying hook 47 can drive the reagent card 51 to sequentially pass through the detection position 44, the sample feeding position 45 and enter the incubation position 46, in a preferred embodiment, the first power assembly 49 is driven by a motor, and the conveying hook 47 has an i-shaped structure; the left and right sides of the transfer hook 47 form a first accommodating space and a second accommodating space respectively, when two reagent cards 51 are placed in the first accommodating space and the second accommodating space respectively, the transfer hook 47 can push the reagent cards 51 to move between the discarding position 42 and the incubation position 46 when moving; in one variation, the first power assembly 49 is driven by a pneumatic cylinder.

Specifically, as shown in fig. 1, 11 and 12, the second material inlet 43 is mainly formed by a lifting mechanism composed of a lifting motor and a lifting plate 48 to lift the reagent card 51, and the main function is to lift the reagent card 51 first, so that the transport hook 47 enters the position shown in fig. 12, and then the reagent card 51 is put down on two sides of the transport hook 47, so that the transport hook 47 can take the reagent card 51 out of the second material inlet 43 and transfer the reagent card to the sample adding position 45, and then the lifting plate 48 is lifted to lift the rest of the reagent card 51 to a first position, and the first position can leave a channel below the lifting plate 48 to facilitate the transport hook 47 to pass the detected reagent card 51 from below the lifting plate 48 into the discarding position 42 for discarding. Or the transfer hook 47 enters the position shown in fig. 1 for the next reagent card 51 removal operation, it should be noted that the cooperation between the transfer hook 47 and the lifting plate 48 must be performed according to the flow path to avoid the collision between the transfer hook 47 and the lifting plate 48. In a preferred embodiment, the lifting device further comprises a detection device, the detection device can detect the movement position of the lifting plate 48, and the detection device can transmit detection information to an upper computer, so that the movement of the conveying motor 52 is controlled, and the collision between the conveying hook 47 and the lifting plate 48 is avoided.

Specifically, as shown in fig. 1, 11 and 13, the incubation bin 60 is internally provided with two incubation sub-bins 70, the incubation sub-bins 70 include two incubation sub-bins and the two incubation sub-bins are respectively arranged at two sides of the incubation bin 70 along the width direction of the support plate 50 at intervals, the incubation sub-bins include a plurality of incubation levels and the incubation levels are arranged in the incubation sub bins at equal intervals in the up-down direction, and the conveying hook 47 entering the incubation position 46 can realize that the reagent card 51 which has been incubated and is completed in the other incubation level is finally conveyed to the discarding position 42 to be discarded after the detection is completed at the detection position 44.

Further, as shown in FIG. 13, the incubation bin 60 includes an incubation bin housing 68 and an incubation motor 69, the incubation chamber 70 is installed inside the incubation chamber housing 68, the inner wall of the incubation chamber housing 68 is provided with a first slide rail along the height direction, the incubation bin 70 is provided with a slide block which is arranged on the first slide rail in a matching way, the incubation motor 69 is mounted above the incubation chamber housing 68 and an incubation drive rod 71 is provided on the incubation motor 69, the incubation drive rod 71 extends through the top of the incubation chamber housing 68 into the interior of the incubation chamber housing 68 and connects to the incubation chamber 70, the incubation position 46 is provided with an incubation position through-hole, when the incubation motor 69 is operated, the incubation driving rod 71 can be driven to move upwards or downwards, and then the incubation bin 70 can be driven to move upwards or downwards through the incubation position through hole under the guidance of the first slide rail.

Furthermore, as shown in fig. 1, 11, and 13, the incubation position 46 is mainly driven by an incubation motor 69 to move up and down by the incubation bin 60, when the transfer hook 47 transfers the reagent card 51 to the sample addition position 45, and the sample is added, the transfer hook 47 directly transfers the reagent card 51 to the incubation bin 60, and then two incubation sub-bins on the incubation bin 60 are lowered one by one, that is, the incubation level drives the reagent card 51 just added with the sample to be lowered one layer, at this time, there are two situations, in one situation, the reagent card 51 with the incubation completed is stored on the upper incubation level, and at this time, the reagent card 51 falls into both sides of the transfer hook 47, so that when the transfer hook 47 is removed from the incubation bin 60, the reagent card 51 with the incubation completed is taken out to the detection position 44, and is taken by the camera, and then the detection position 44 is removed to discard the reagent card 51. In another case, if the previous incubation layer is empty, i.e., no reagent card 51 is stored, the transfer hook 47 moves back to enter the second feeding position 43 to take off one reagent card 51, and then the sample is dropped from the sample feeding position 45 and then conveyed to the incubation layer for incubation, and so on, thereby ensuring the continuity of the incubation operation.

The incubation bin 70 of the invention adopts a lifting structure, so that the whole incubation bin is kept at a constant temperature under a rated temperature, and meanwhile, the left and right incubation sub-bins are respectively arranged in a symmetrical structure, so that the detection flux can be increased. While the first reagent card 51 is loaded and then directly enters the incubation sub-chamber for waiting for 10 minutes for testing, the reagent card transport mechanism can continue to take out the next reagent card 51 from the loading position 2 for loading and then enter the incubation chamber 70. In a preferred embodiment, 15 incubation layers are designed in one incubation sub-bin, so that continuous 15 samples can be ensured to enter for incubation in sequence, and continuous detection is achieved by adopting the work of a pipeline mode.

Specifically, as shown in fig. 1, 11, 13 and 14, the reagent card transfer mechanism comprises a transfer guide rod 57, a transmission carrier 58 and a second power assembly, the stage 41 further comprises a third support leg 62 and a fourth support leg 63, and the third support leg 62 and the fourth support leg 63 are respectively installed at two ends of the lifting plate 48; the two ends of the transmission guide rod 57 are respectively arranged on the third supporting leg 62 and the fourth supporting leg 63 and are arranged below the lifting plate 48; a transmission carrier through hole 59 is formed in the transmission carrier 58, the lower end of the transmission carrier 58 is sleeved on the transmission guide rod 57 through the transmission carrier through hole 59 and can slide relative to the transmission guide rod 57, and the upper end of the transmission carrier 58 is connected with the transmission hook 47; the second power assembly can drive the transmission carrier 58 to slide in the direction of the transmission guide rod 57 and simultaneously drive the transmission hook 47 to slide above or below the lifting plate 48.

Further, as shown in fig. 1, 11, 13 and 14, the second power assembly includes a transmission motor 52, a third timing belt 53, a first driving synchronizing wheel 54, a fourth timing belt 55, a driven synchronizing wheel 56, an upper computer and a second driving synchronizing wheel 61; one end of the third synchronous belt 53 is connected with the transmission motor 52, the other end of the third synchronous belt 53 is sleeved on the first driving synchronous wheel 54, one end of the fourth synchronous belt 55 is sleeved on the second driving synchronous wheel 61, the other end of the fourth synchronous belt 55 is sleeved on the driven synchronous wheel 56, and one side of the fourth synchronous belt 55 is connected with the transmission carrier 58; the first driving synchronous wheel 54 and the second driving synchronous wheel 61 are respectively arranged at two ends of a connecting shaft, and the connecting shaft is rotatably arranged on a support 59; when the transmission motor 52 rotates, the third synchronous belt 53 can be driven to drive the first driving synchronous wheel 54 to rotate, and then the second driving synchronous wheel 61 can be driven to synchronously rotate and drive the other end of the fourth synchronous belt 55 to move around the driven synchronous wheel 56, at this time, the transmission carrier 58 follows the fourth synchronous belt 55 to move.

Specifically, the conveying motor 52 is provided with an encoder, and the encoder is electrically connected with an upper computer. Reagent card transport mechanism mainly drives the synchronizing wheel by conveying motor 52 and drives the hold-in range motion by the synchronizing wheel motion again, and conveying hook 47 is connected with the hold-in range, like this along with the rotation of motor, can remove conveying hook 47 to any point position, when needing to pay attention to, conveying motor 52 is the closed loop step motor who takes the encoder, and the position that conveying hook 47 belongs to all can be obtained by the encoder and send the host computer record to in the database. Due to the fact that various unknown factors cause the power failure of the equipment or the fault of the equipment, the position of the transfer hook 47 can be read from the database, the logical relationship between the second feeding position 43 and the incubation position 46 and the transfer hook 47 in subsequent operation is convenient, and errors can be avoided.

It should be noted that the key to the cooperation between the reagent card transport mechanism and the elevator of the incubation chamber and the cooperation between the reagent card transport mechanism and the elevator of the second feeding position 43 is in the present invention, since the reagent card transport mechanism transports the reagent card 51 in the horizontal direction and the incubation chamber 70 and the second feeding position 43 transport the reagent card 51 in the vertical direction, the phenomena of jamming, bumping, etc. are easily generated at the junction due to improper control. Therefore, the incubation motor 69 is also in signal connection with the upper computer, and the upper computer performs logic judgment through the obtained position of the conveying hook 47 and forms a control relation with the incubation motor 69, so that the reliability of motion connection is ensured. Meanwhile, the program can timely feed back where the fault occurs when the fault occurs accidentally.

Specifically, as shown in fig. 12, a rectangular hole is formed in the middle of the supporting plate 50 along the length direction, and the upper end of the transmission carrier 58 passes through the rectangular hole to be connected with the conveying hook 47 and can drive the conveying hook 47 to move along the direction of the rectangular hole.

Specifically, as shown in fig. 12, the disposal position 42 is provided with two disposal through holes, the two disposal through holes respectively correspond to the first accommodating space and the second accommodating space in the vertical direction, and when the transfer hook 47 drives the detected reagent card 51 to move to the disposal through hole, the reagent card 51 is discarded and recovered after dropping from the disposal through hole.

Specifically, as shown in fig. 11 and 12, a first limit plate 64 and a second limit plate 65 are respectively disposed on two sides of the second material feeding position 43 along the length direction of the support plate 50, the first limit plate 64 and the second limit plate 65 are respectively mounted on the support plate 50, and a first gap and a second gap are respectively disposed between the first limit plate 64 and the support plate 50, wherein the first gap and the second gap are passages for the transfer hook 47 to move on the support plate 50, the second material feeding position 43 is provided with a first lifting hole and a second lifting hole, and the first lifting hole and the second lifting hole are respectively disposed on two sides of the rectangular hole, the number of the lifting plates 48 is two, the two lifting plates 48 can synchronously move between the first position and the second position through the first lifting hole and the second lifting hole respectively, and when the two lifting plates 48 move above the support plate 50, the two lifting plates 48 can move in a space between the first limit plate 64 and the second limit plate 65, the first and second stopper plates 64 and 65 function to restrict and guide the movement of the lift plate 48, and contribute to the stability of the movement of the lift plate 48.

Specifically, as shown in fig. 11 and 13, a camera 67 and a dark room 66 are arranged on the detection position 44, the camera 67 is mounted on the support plate 50 through the dark room 66, the dark room 66 is mounted on the support plate 50, and a third gap is arranged between the camera and the support plate 50, wherein the third gap is a passage for the transfer hook 47 to move on the support plate 50; the camera 67 is capable of photographing the reagent card 51 placed in the camera 66 at the detection position 44 through the photographing through hole formed in the camera 66.

Specifically, as shown in fig. 1 and fig. 2, the X-axis sample loading mechanism 200 includes a sample loading support housing 74, a sample loading moving assembly, and a plunger pump 75; the sample adding supporting shell 74 is provided with a second slide rail 76, and the sample adding movement component comprises an X-direction movement bracket 78, an X-direction motor 81, a fourth synchronous belt 82, an X-direction driving wheel 83 and an X-direction driven wheel 84; the second slide rail 76, the X-direction motor 81, the X-direction driving wheel 83 and the X-direction driven wheel 84 are all arranged on the sample adding supporting shell 74; two ends of the fourth synchronous belt 82 are respectively sleeved on the X-direction driving wheel 83 and the X-direction driven wheel 84; the X-direction moving bracket 78 is mounted on the second slide rail 76 and can slide on the second slide rail 76, and the X-direction moving bracket 78 is connected with the fourth synchronous belt 82; the X-direction motor 81 and the X-direction driving wheel 83 are respectively installed at two sides of the sample-adding supporting housing 74, and the X-direction motor 81 can drive the X-direction driving wheel 83 to rotate to drive the fourth synchronous belt 82 to drive the X-direction moving support 78 to slide along the length direction of the second slide rail 76.

Specifically, as shown in fig. 2, the sample adding movement assembly includes a Z-direction motor 77, a third driving screw 79, a third slide rail 80 and a Z-direction movement bracket 85, the Z-direction motor 77 and the third slide rail 80 are both mounted on the X-direction movement bracket 78, and the Z-direction movement bracket 85 is mounted on the third slide rail 80 and can slide along the third slide rail 80; one end of the third driving screw 79 is connected with the Z-direction motor 77, the other end of the third driving screw 79 is connected with the Z-direction moving bracket 85, and when the Z-direction motor 77 rotates, the third driving screw 79 rotates to drive the Z-direction moving bracket 85 to vertically move upwards or downwards along the third slide rail 80; the TIP needle 73 is mounted on a Z-motion carriage 85, and the plunger pump 75 is connected to the TIP needle 73 via a tube.

Specifically, as shown in fig. 1 and 3, the TIP transport mechanism 300 includes a TIP cassette 86, a TIP cassette transport motor 87, a TIP cassette transport driving pulley 88, a TIP cassette transport driven pulley 89, a TIP cassette transport support table 90, a TIP cassette transport link frame 91, and a TIP cassette timing belt 93; the TIP box conveying support platform 90 is provided with a TIP box support plate, the TIP box 86 is arranged on the TIP box support plate through a TIP box sliding plate 92, the TIP box conveying linkage frame 91 is arranged below the TIP box support plate, two ends of the TIP box conveying linkage frame extend outwards and upwards and are respectively connected with two ends of the TIP box sliding plate 92; the TIP box transmission linkage frame 91 is connected with a TIP box synchronous belt 93; the TIP box transmission motor 87, the TIP box transmission driving wheel 88 and the TIP box transmission driven wheel 89 are all arranged on the TIP box transmission support platform 90, and two ends of the TIP box synchronous belt 93 are respectively sleeved on the TIP box transmission driving wheel 88 and the TIP box transmission driven wheel 89; when the TIP box conveying motor 87 rotates, the TIP box conveying driving wheel 88 can be driven to rotate and drive the TIP box synchronous belt 93 to move, and then the TIP box conveying linkage frame 91 is driven to drive the TIP box sliding plate 92 and the TIP box 86 to move on the TIP box supporting plate.

The working principle of the invention is as follows:

firstly, placing a plurality of sample racks 4 in a sample rack storage area 3, controlling a first motor 6 to start to drive a driving push plate 18 to push the sample rack 4 closest to a first through hole 20 to a bearing space on a transmission carrier 5 at a first feeding position 34 by a control device, controlling a second motor 7 to start by the control device, and driving a first driving screw 8 to rotate by the second motor 7 so as to enable the transmission carrier 5 to move to a liquid adding extrusion position 35; the control device controls the liquid adding motor 19 to start and drives the sample clamping arm 30 to rotate along the axial direction of the liquid adding motor 19, so that the sample adding needle 24 arranged on the sample clamping arm 30 moves to the position right above the opening part of the sample tube 31, and at the moment, the sample tube 31 is added with diluent through a peristaltic pump connected with the sample adding needle 24; after the sample tube 31 is added with the diluent, the control device controls the second motor 7 to drive the transmission carrier 5 to continue to move and pass through a narrow channel formed by the two groups of clamping heads 22, so that the sample in the sample tube 31 is extruded and uniformly mixed and then moves to the discharging position 36, and then the second motor 7 stops rotating; the control device controls the discharging motor 25 to start and drives the second driving screw 27 to drive the discharging push rod 26 to move along the length direction of the second driving screw 27, so as to push the sample rack into the sample outlet position.

Secondly, the control device controls the rotation of the TIP box conveying motor 87 so as to drive the TIP box conveying driving wheel 88 to rotate and drive the TIP box synchronous belt 93 to move, the TIP box synchronous belt 93 is connected with the TIP box conveying linkage frame 91 so as to drive the TIP box conveying linkage frame 91 to drive the TIP box sliding plate 92 and the TIP box 86 to move on the TIP box supporting plate, and when the TIP box conveying motor 87 moves to the lower part of the Z-direction moving support 85, the control device controls the TIP box conveying motor 87 to stop rotating.

Thirdly, the control device controls the first power assembly 49 to lift the reagent card 51 on the second feeding position 43, the conveying hook 47 enters the second feeding position 43 from the bottom of the reagent card 51, the reagent card 51 descends to enable the reagent card 51 to enter the working range of the feeding hook 7, then the feeding hook 7 pushes the reagent card 51 to pass through the detection position 44 to enter the sample feeding position 45, at the moment, the control device controls the X-direction motor 81 to drive the motion bracket 78 to move until the TIP needle rod 73 moves to be right above one TIP head 72 on the TIP box 86, at the moment, the control device controls the Z-direction motor 77 to start and drive the TIP needle rod 73 to move close to the TIP head 72 until the TIP head 72 is mounted on the TIP needle rod 73, after the Z-direction motion bracket 85 drives the TIP head 72 to move upwards to a certain height, the control device controls the X-direction motor 81 to drive the motion bracket 78 to move to be right above the sample rack 4, at the moment, the control device controls the X-direction motor 81 to, until the TIP head 72 extends into the sample tube 31 to suck the sample into the TIP needle rod 73 for storage, the control device controls the Z-direction moving bracket 85 to drive the TIP head 72 to move upwards to a certain height, and then controls the X-direction motor 81 to drive the moving bracket 78 to move towards the direction close to the sample adding position 45.

Finally, when the TIP head 72 moves above the sample application site 45, the control device controls the plunger pump 75 to apply the sample to the reagent card 51 moved to the sample application site 45. After loading of sample has been completed transfer hook 47 continues to transfer reagent card 51 to the first incubation level of incubation magazine 60, incubation magazine 60 lowers transfer hook 47 out of the lowered second incubation level into a loading position to continue to bring second reagent card 51 into loading position 45 for loading into the second incubation level of incubation magazine 60, and so on. When the first incubation position is finished, the conveying hook 47 takes the reagent card 51 out of the incubation bin 60, enters the detection position 44 for photo detection, and then directly enters the discarding position 42 to discard the reagent card 51 from the second gap, the first gap and the lifting plate 48 on the two sides of the second feeding position 43, and the used TIP head 72 is discarded and then a new TIP head 72 is installed for use.

The invention completely integrates the functions of sample pretreatment, automatic sample adding, automatic incubation, automatic detection and the like, replaces the manual work to realize the automation of the whole flow, and reduces the influence of human factors.

In the description of the present application, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience in describing the present application and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present application.

The foregoing description of specific embodiments of the present invention has been presented. It is to be understood that the present invention is not limited to the specific embodiments described above, and that various changes or modifications may be made by one skilled in the art within the scope of the appended claims without departing from the spirit of the invention. The embodiments and features of the embodiments of the present application may be combined with each other arbitrarily without conflict.

Claims

1. A multifunctional full-automatic colloidal gold detector is characterized by comprising a feeding mechanism (100), an X-axis sample adding mechanism (200), a TIP conveying mechanism (300), an incubation mechanism (400), a detector mounting table (500) and a control device;

the feeding mechanism (100), the X-axis sample adding mechanism (200), the TIP conveying mechanism (300) and the incubation mechanism (400) are all arranged on a detector mounting table (500);

the feeding mechanism (100) comprises a sample rack (4), a sample tube (31) is mounted on the sample rack (4), and under the control of the control device, the feeding mechanism (100) can add a diluent into the sample tube (31) and uniformly mix the diluent to form a standby sample;

the incubation mechanism (400) comprises a reagent card (51) and an incubation magazine (60);

the TIP transmission mechanism (300) is provided with a TIP head (72);

the X-axis sample adding mechanism (200) is provided with a TIP needle rod (73), under the control of the control device, the X-axis sample adding mechanism (200) can install a TIP head (72) on the TIP needle rod (73), can take out a sample from the sample tube (31) through the TIP head (72) and add the sample to the reagent card (51), and conveys the reagent card (51) with the sample added to the incubation bin (60) to finish detection in the incubation mechanism (400) after incubation is finished.

2. The multifunctional full-automatic colloidal gold detector according to claim 1, wherein the feeding mechanism (100) comprises a bearing frame (1), a first driving device, a second driving device, a liquid adding device, a squeezing device and a discharging device;

the loading plate (2) is mounted on the loading frame (1), and the loading plate (2) sequentially comprises a first feeding position (34), a liquid feeding extrusion position (35) and a discharging position (36) along the length direction;

the first driving device and the discharging device are respectively arranged on a first feeding position (34) and a discharging position (36), and the liquid adding device and the extruding device are respectively arranged on a liquid adding extruding position (35);

the second driving device is arranged on the bearing frame (1) and is provided with a transmission carrier (5) and a second motor (7), a first through hole (20) is formed in the middle of the bearing plate (2) along the length direction of the bearing plate (2), a bearing space is formed in the upper end of the transmission carrier (5) and is arranged on the bearing plate (2), the lower end of the transmission carrier (5) penetrates through the first through hole (20) to extend to the lower side of the bearing plate (2) and is in driving connection with the second motor (7), and the sample frame (4) can be installed in the bearing space in a matched mode;

the first feeding position (34) is connected with a sample rack storage area (3), and a sample rack (4) placed in the sample rack storage area (3) can be conveyed to the bearing space under the driving of the first driving device;

under the driving of a second motor (7), the transmission carrier (5) can drive the sample rack (4) to move to a discharging position (36) from a first feeding position (34) through a liquid adding extrusion position (35), and the sample rack (4) moving to the discharging position (36) can be pushed out to leave the discharging position (36) under the driving of a discharging device;

the sample rack (4) comprises a sample rack base (32) and a sample tube carrier (33);

the sample tube carrier (33) is connected with the sample frame base (32) through a plurality of connecting ribs;

the utility model discloses a sample frame, including sample frame base (32), sample pipe bearing body (33), sample frame base (32) are gone up the equidistance in proper order and are provided with the mounting groove of a plurality of indents, sample pipe bearing body (33) are gone up the equidistance in proper order and are provided with the space of placing of a plurality of cylindrics, place space and mounting groove one-to-one from top to bottom, install in the mounting groove through placing the space when placing sample pipe (31), wherein, the one end setting of splice bar is between two adjacent mounting grooves, the other end setting of splice bar is between two adjacent spaces of placing, the width of splice bar is less than the.

3. The multifunctional full-automatic colloidal gold tester according to claim 2, wherein the second driving device further comprises a first driving lead screw (8), a first bearing seat (9) and a second bearing seat (10);

the utility model discloses a bearing plate, including bearing board (2), first bearing frame (9), second bearing frame (10), the lower extreme of transmission carrier (5) is provided with the internal thread hole, be provided with on first drive lead screw (8) with internal thread hole assorted external screw thread, the one end of first drive lead screw (8) is installed on first bearing frame (9), the other end of first drive lead screw (8) passes internal thread hole, second bearing frame (10) in proper order and is connected with second motor (7) drive, second motor (7) and the drive mode of first drive lead screw (8) include any one of following structural style:

-using a synchronous belt drive;

-using a coupling for transmission;

-using a gear drive;

the first driving device comprises a first motor (6), a protection plate (13), a first belt wheel (14), a first synchronous belt (15), a second belt wheel (16), a transmission piece (17) and a driving push plate (18);

the driving push plate (18) is arranged on the sample rack storage area (3), the second belt wheel (16) is arranged on the side surface of the sample rack storage area (3), and the transmission piece (17) is arranged on the first synchronous belt (15) and connected with the driving push plate (18);

one end of the first synchronous belt (15) is sleeved on the second belt wheel (16), the other end of the first synchronous belt (15) is sleeved on the first belt wheel (14), and the first belt wheel (14) is in driving connection with the first motor (6);

when the first motor (6) rotates, the first belt wheel (14) can be driven to rotate and drive the first synchronous belt (15) to move so as to drive the transmission piece (17) to drive the driving push plate (18) to push the sample rack (4) placed on the sample rack storage area (3) into the bearing space;

the utility model discloses a protection plate, including protection plate (13), first motor (6), first band pulley (14), driving medium (17), drive push pedal (18) are installed respectively in the both sides of protection plate (13), be provided with second through-hole (21) on protection plate (13), driving medium (17) are connected with drive push pedal (18) that pass second through-hole (21).

4. The multifunctional full-automatic colloidal gold detector according to claim 2, wherein the liquid adding device comprises a liquid adding motor (19) and a sample adding component, a sample clamping arm (30), a peristaltic pump, a stepping motor and a sample adding needle (24) are arranged on the sample adding component, and the peristaltic pump is in driving connection with the stepping motor;

when the sample rack (4) moves to the liquid adding extrusion position (35), the sample clamping arm (30) clamping the sample adding needle (24) can move to the upper part of the sample rack (4) under the driving of the liquid adding motor (19) and is connected with the sample adding needle (24) through the peristaltic pump to complete liquid adding to the sample tube (31) arranged on the bearing frame (1);

the extrusion device comprises a clamping head (22), a fixing plate (23) and a spring;

the clamping head (22) and the fixing plate (23) are both arranged on the liquid adding extrusion position (35);

the number of the clamping heads (22) is four, wherein two clamping heads (22) are arranged on one side of the first through hole (20), the other two clamping heads (22) are arranged on the other side of the first through hole (20), the other two clamping heads (22) are connected with the fixing plate (23), and springs are arranged inside the other two clamping heads (22);

the clamping heads (22) on the two sides correspond to each other in pairs, a narrow channel is formed between every two corresponding clamping heads (22), and the width of the narrow channel is smaller than the diameter of the sample tube (31);

the discharging device comprises a discharging motor (25), a discharging push rod (26), a second driving screw rod (27), a discharging base (28) and a discharging guide rod (29);

the discharging base (28) is installed on a discharging position (36), the discharging motor (25) is installed on the discharging base (28), two discharging through holes are respectively formed in the discharging base (28) and are respectively arranged on two sides of the discharging motor (25), one ends of the two discharging guide rods (29) are respectively installed on the discharging push rod (26), and the other ends of the two discharging guide rods (29) are respectively installed in the two discharging through holes and can slide relative to the discharging through holes;

one end of the second driving lead screw (27) is installed on the discharging push rod (26), the other end of the second driving lead screw (27) is connected with the discharging motor (25), when the discharging motor (25) runs, the second driving lead screw (27) can be driven to drive the discharging push rod (26) to move along the length direction of the second driving lead screw (27), and meanwhile, the two discharging guide rods (29) move along with the discharging push rod (26) and slide in the two discharging through holes.

5. The multi-functional fully-automatic colloidal gold meter according to claim 1, wherein the incubation mechanism (400) further comprises a reagent card transfer mechanism and a stage (41);

the object stage (41) is provided with a supporting plate (50), the supporting plate (50) sequentially comprises a discarding position (42), a second feeding position (43), a detecting position (44), a sample adding position (45) and an incubation position (46) towards the direction close to the incubation bin (60), wherein the incubation position (46) extends into the incubation bin (60);

the reagent card transfer mechanism comprises a transfer hook (47), the transfer hook (47) is mounted on a support plate (50) and can move between a disposal position (42) and an incubation position (46) under the driving of the reagent card transfer mechanism;

a lifting plate (48) and a first power assembly (49) are arranged on the second feeding position (43), the reagent card (51) is placed on the lifting plate (48), the lifting plate (48) can move between a first position and a second position under the driving of the first power assembly (49), and the first power assembly (49) is driven by a motor or a cylinder;

when the lifting plate (48) drives the reagent card (51) to move to the first position, the conveying hook (47) is driven by the reagent card conveying mechanism to move to the second feeding position (43), at the moment, the lifting plate (48) is driven by the first power assembly (49) to move to the second position, the reagent card (51) enters the working range of the conveying hook (47), and the conveying hook (47) can drive the reagent card (51) to sequentially pass through the detection position (44), the feeding position (45) and enter the incubation position (46);

the incubation bin (60) is internally provided with an incubation bin (70), the incubation bin (70) comprises two incubation sub-bins which are respectively arranged on two sides of the inside of the incubation bin (70) at intervals along the width direction of the supporting plate (50), the incubation sub-bins comprise a plurality of incubation layers which are arranged in the incubation sub-bins at equal intervals along the up-down direction, and a conveying hook (47) entering the incubation position (46) can drive a reagent card (51) which is already incubated on the other incubation layer to move to the detection position (44) and finally convey the detected reagent card (51) to the discarding position (42) for discarding after the detection is completed;

the incubation bin (60) comprises an incubation bin housing (68) and an incubation motor (69);

the incubation well (70) is mounted inside an incubation well housing (68);

a first sliding rail is arranged on the inner wall of the incubation bin shell (68) along the height direction, a sliding block is arranged on the incubation bin (70), and the sliding block is installed on the first sliding rail in a matching mode;

the incubation motor (69) is arranged above the incubation chamber shell (68) and an incubation driving rod (71) is arranged on the incubation motor (69);

the incubation driving rod (71) penetrates through the top of the incubation chamber shell (68), extends into the incubation chamber shell (68) and is connected with the incubation chamber (70), an incubation position through hole is formed in the incubation position (46), when the incubation motor (69) is operated, the incubation driving rod (71) can be driven to move upwards or downwards, and then the incubation chamber (70) can be driven to move upwards or downwards through the incubation position through hole under the guidance of the first sliding rail;

a darkroom (66) and a camera (67) are arranged on the detection position (44);

the camera (67) is mounted on the support plate (50) through a darkroom (66);

the darkroom (66) is arranged on the supporting plate (50) and a third gap is arranged between the darkroom and the supporting plate (50); the darkroom (66) is provided with a shooting through hole, and the camera (67) can shoot the reagent card (51) placed on the detection position (44) through the shooting through hole.

6. The multi-functional fully-automatic colloidal gold measuring instrument according to claim 5, wherein said reagent card transport mechanism further comprises a transport guide rod (57), a transmission carrier (58), and a second power assembly;

the object stage (41) further comprises a third supporting leg (62) and a fourth supporting leg (63), wherein the third supporting leg (62) and the fourth supporting leg (63) are respectively installed at two ends of the lifting plate (48);

the two ends of the transmission guide rod (57) are respectively arranged on a third supporting leg (62) and a fourth supporting leg (63) and are arranged below the lifting plate (48);

a transmission carrier through hole (59) is formed in the transmission carrier (58), the lower end of the transmission carrier (58) is sleeved on the transmission guide rod (57) through the transmission carrier through hole (59) and can slide relative to the transmission guide rod (57), and the upper end of the transmission carrier (58) is connected with the transmission hook (47);

the second power assembly can drive the transmission carrier (58) to slide along the direction of the transmission guide rod (57) and simultaneously drive the transmission hook (47) to slide on the lifting plate (48);

the second power assembly comprises a transmission motor (52), a third synchronous belt (53), a first driving synchronous wheel (54), a fourth synchronous belt (55), a driven synchronous wheel (56), an upper computer and a second driving synchronous wheel (61);

one end of the third synchronous belt (53) is connected with the conveying motor (52), the other end of the third synchronous belt (53) is sleeved on the first driving synchronous wheel (54), one end of the fourth synchronous belt (55) is sleeved on the second driving synchronous wheel (61), the other end of the fourth synchronous belt (55) is sleeved on the driven synchronous wheel (56), and one side of the fourth synchronous belt (55) is connected with the transmission carrier (58);

the first driving synchronizing wheel (54) and the second driving synchronizing wheel (61) are respectively arranged at two ends of the connecting shaft, and the connecting shaft is rotatably arranged on the support (59);

when conveying motor (52) rotated, can drive third hold-in range (53) drive first initiative synchronizing wheel (54) and rotate, and then can drive second initiative synchronizing wheel (61) synchronous revolution and drive the other end of fourth hold-in range (55) around driven synchronizing wheel (56) motion, and fourth hold-in range (55) motion is followed in transmission carrier (58) this moment, wherein be provided with the encoder on conveying motor (52), the encoder is connected with host computer electricity.

7. The multifunctional full-automatic colloidal gold detecting instrument according to claim 6, wherein the middle part of the supporting plate (50) is provided with a rectangular hole along the length direction, the upper end of the transmission carrier (58) passes through the rectangular hole to be connected with the conveying hook (47) and can drive the conveying hook (47) to move along the direction of the rectangular hole;

the conveying hook (47) is of an I-shaped structure; the left side and the right side of the conveying hook (47) form a first accommodating space and a second accommodating space respectively, when two reagent cards (51) are placed into the first accommodating space and the second accommodating space respectively, the conveying hook (47) can push the reagent cards (51) to move between the discarding position (42) and the incubation position (46) when moving;

two discarding through holes are formed in the discarding position (42), and correspond to the first accommodating space and the second accommodating space in the vertical direction respectively.

8. The multifunctional full-automatic colloidal gold detector according to claim 1, wherein the X-axis sample loading mechanism (200) comprises a sample loading support housing (74) and a sample loading moving assembly;

a second slide rail (76) is arranged on the sample adding support shell (74);

the sample adding movement assembly comprises an X-direction movement bracket (78), an X-direction motor (81), a fourth synchronous belt (82), an X-direction driving wheel (83) and an X-direction driven wheel (84);

the second slide rail (76), the X-direction motor (81), the X-direction driving wheel (83) and the X-direction driven wheel (84) are all arranged on the sample adding supporting shell (74);

two ends of the fourth synchronous belt (82) are respectively sleeved on the X-direction driving wheel (83) and the X-direction driven wheel (84);

the X-direction moving support (78) is mounted on a second sliding rail (76) and can slide on the second sliding rail (76), and the X-direction moving support (78) is connected with a fourth synchronous belt (82);

x is to motor (81), X to action wheel (83) install respectively in the both sides of application of sample support casing (74) and X can drive X to action wheel (83) and rotate and drive fourth hold-in range (82) and drive X to the direction slip of motion support (78) along second slide rail (76) length to motor (81).

9. The multifunctional full-automatic colloidal gold detector according to claim 8, wherein the sample-adding moving assembly comprises a plunger pump (75), a Z-direction motor (77), a third driving screw (79), a third slide rail (80) and a Z-direction moving bracket (85);

the Z-direction motor (77) and the third slide rail (80) are both arranged on the X-direction moving support (78), and the Z-direction moving support (85) is arranged on the third slide rail (80) and can slide along the third slide rail (80);

one end of the third driving screw rod (79) is connected with the Z-direction motor (77), the other end of the third driving screw rod (79) is connected with the Z-direction moving support (85), and when the Z-direction motor (77) rotates, the third driving screw rod (79) rotates to drive the Z-direction moving support (85) to vertically move upwards or downwards along the third sliding rail (80);

the TIP needle rod (73) is arranged on a Z-direction movement bracket (85), and the plunger pump (75) is connected with the TIP needle rod (73) through a pipeline.

10. The multi-functional full-automatic colloidal gold meter according to claim 1, wherein the TIP transport mechanism (300) comprises a TIP cassette (86), a TIP cassette transport motor (87), a TIP cassette transport driving wheel (88), a TIP cassette transport driven wheel (89), a TIP cassette transport support table (90), a TIP cassette transport linkage frame (91) and a TIP cassette synchronous belt (93);

the TIP box conveying support platform (90) is provided with a TIP box support plate, the TIP box (86) is installed on the TIP box support plate through a TIP box sliding plate (92), the TIP box conveying linkage frame (91) is installed below the TIP box support plate, two ends of the TIP box conveying linkage frame extend outwards and upwards and are respectively connected with two ends of the TIP box sliding plate (92);

the TIP box transmission linkage frame (91) is connected with a TIP box synchronous belt (93);

the TIP box conveying motor (87), the TIP box conveying driving wheel (88) and the TIP box conveying driven wheel (89) are all arranged on the TIP box conveying support platform (90), and two ends of the TIP box synchronous belt (93) are respectively sleeved on the TIP box conveying driving wheel (88) and the TIP box conveying driven wheel (89);

when the TIP box transmission motor (87) rotates, the TIP box transmission driving wheel (88) can be driven to rotate and drive the TIP box synchronous belt (93) to move, and then the TIP box transmission linkage frame (91) is driven to drive the TIP box sliding plate (92) and the TIP box (86) to move on the TIP box supporting plate.