CN113671201B - Automatic partial shipment device of biological sample - Google Patents
Automatic partial shipment device of biological sample Download PDFInfo
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- CN113671201B CN113671201B CN202110873298.7A CN202110873298A CN113671201B CN 113671201 B CN113671201 B CN 113671201B CN 202110873298 A CN202110873298 A CN 202110873298A CN 113671201 B CN113671201 B CN 113671201B
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- 238000005070 sampling Methods 0.000 claims abstract description 134
- 239000002699 waste material Substances 0.000 claims abstract description 61
- 239000007788 liquid Substances 0.000 claims abstract description 59
- 239000000523 sample Substances 0.000 claims abstract description 48
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 47
- 239000010902 straw Substances 0.000 claims abstract description 29
- 238000005192 partition Methods 0.000 claims description 37
- 238000006243 chemical reaction Methods 0.000 claims description 15
- 230000001360 synchronised effect Effects 0.000 claims description 15
- 230000005540 biological transmission Effects 0.000 claims description 12
- 230000000149 penetrating effect Effects 0.000 claims description 5
- 238000005213 imbibition Methods 0.000 claims description 4
- 238000007790 scraping Methods 0.000 claims description 3
- 238000001514 detection method Methods 0.000 abstract description 26
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- 208000015181 infectious disease Diseases 0.000 description 4
- 238000012423 maintenance Methods 0.000 description 4
- 238000003199 nucleic acid amplification method Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 230000017525 heat dissipation Effects 0.000 description 3
- 238000004064 recycling Methods 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/026—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having blocks or racks of reaction cells or cuvettes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
- G01N2035/0401—Sample carriers, cuvettes or reaction vessels
- G01N2035/0403—Sample carriers with closing or sealing means
- G01N2035/0405—Sample carriers with closing or sealing means manipulating closing or opening means, e.g. stoppers, screw caps, lids or covers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
- G01N2035/0439—Rotary sample carriers, i.e. carousels
- G01N2035/0444—Rotary sample carriers, i.e. carousels for cuvettes or reaction vessels
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
- G01N2035/0474—Details of actuating means for conveyors or pipettes
- G01N2035/0482—Transmission
- G01N2035/0484—Belt or chain
Landscapes
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Sampling And Sample Adjustment (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention discloses an automatic split charging device for biological samples, which comprises: a frame; the first working platform comprises a first linear movement mechanism and a sampling tube operating mechanism, wherein the first linear movement mechanism is fixed on the frame and drives the sampling tube operating mechanism to move along an X axis, and the sampling tube operating mechanism is used for placing a sampling tube and clamping or loosening the sampling tube; the second working platform comprises a second linear motion mechanism and a cup separating operation mechanism, the second linear motion mechanism is fixed on the rack and drives the cup separating operation mechanism to move along the X axis, and the cup separating operation mechanism is used for placing a sample box, a reagent tube, a straw box and a waste straw; the cap screwing mechanism is used for screwing the cap body of the sampling tube; the liquid suction mechanism is used for carrying a suction pipe to suck liquid, drip liquid and peel off the waste pipe. The device collects bar code scanning, spiral cover, screw up the lid, pipetting partial shipment, useless tub recovery function in an organic whole, degree of automation is high, pollute little, and detection precision and efficient, and be convenient for transport and put in use, the range of application is wide.
Description
Technical Field
The invention belongs to the technical field of medical instruments, and particularly relates to an automatic split charging device for biological samples.
Background
With the wide application of molecular biology technology in biology, medicine and other related fields, the detection and analysis of nucleic acids are increasingly used in genetic diseases, tumors, infectious diseases and the like. The nucleic acid detection principle is based on real-time fluorescent quantitative polymerase chain reaction (Polymerase Chain Reaction, PCR), a PCR instrument is a basic instrument in genetic engineering and biological experiments, and is mainly used for concentration amplification of DNA, a low concentration sample can be amplified into a high concentration sample, and the original concentration of nucleic acid can be calculated from the data in the amplification process. In modern manufacturing industry, detection technology is widely applied, detection means and methods are increasingly more, and thus, higher requirements are placed on the efficiency of detection equipment, but the existing nucleic acid detectors still have the following defects:
1) The automatic degree is low, the split charging efficiency is low, manual detection is mostly adopted, pipetting, nucleic acid extraction, purification, amplification and detection are all performed manually from a sampling tube, secondary infection risks exist, personnel safety is difficult to ensure, the sampling tubes are usually detected one by one, namely, the sampling tubes are manually moved into an instrument and then manually moved out after detection is finished, a large amount of medical resources are occupied, the detection intensity is difficult to adapt to occasions with high flux, and errors are easy to occur;
2) The output of results is slow, diagnosis and treatment are affected, and the instrument is single in function, large in occupied space, inconvenient to transport and limited in use field;
3) Biological safety is difficult to guarantee, in the process of nucleic acid detection, biological pollution such as aerosol, liquid leakage and the like can be generated in the processes of screwing, pipetting, nucleic acid extraction and amplification and the like of sample liquid, and once pollution occurs, false positive results can be caused, so that personnel safety can be possibly endangered.
Therefore, there is an urgent need to develop an efficient automatic split charging device for biological samples.
Disclosure of Invention
Aiming at the problems, the invention provides an automatic split charging device for biological samples, which integrates the functions of bar code scanning, cap screwing, liquid transferring split charging and waste pipe recycling, can realize full-automatic split charging, reduce manual labor, reduce the pollution of biological samples and the risk of secondary infection of personnel, greatly improve the detection precision and efficiency, has compact structure, convenient operation, convenient transportation, throwing and use and wide application range, and is particularly suitable for occasions with high detection intensity and large flux.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:
the invention provides an automatic split charging device for biological samples, which comprises:
A frame;
the first working platform comprises a first linear motion mechanism and a sampling tube operating mechanism, wherein the first linear motion mechanism is fixed on the frame and drives the sampling tube operating mechanism to move along the X axis, and the sampling tube operating mechanism comprises a first placing platform, a sampling tube positioning mechanism and a sampling tube clamping mechanism, wherein the first linear motion mechanism comprises a first positioning platform, a first positioning platform and a second positioning platform, wherein the first positioning platform is fixedly connected with the first positioning platform:
the first placing platform is connected with the first linear motion mechanism;
the sampling tube positioning mechanism is arranged on the first placing platform and comprises a first cover plate, a clamping plate mechanism, a first bottom frame and a first partition plate, wherein the first cover plate is connected with the first bottom frame, the clamping plate mechanism and the first partition plate are internally arranged in the first bottom frame, a plurality of first through holes are formed in the first cover plate, a plurality of second through holes are formed in the clamping plate mechanism, a plurality of third through holes are formed in the first partition plate, the first through holes, the second through holes and the third through holes are coaxially arranged and are sequentially distributed along the axial direction in an initial state and are used for placing sampling tubes, the sampling tubes are used for accommodating biological samples, a plurality of blade grooves are formed in the inner side of the lower wall of the first bottom frame and correspondingly distributed below the third through holes, blades are internally arranged in the blade grooves, a plurality of first avoidance grooves corresponding to the blades are formed in the first partition plate, and a second avoidance groove is formed in the side wall of the first bottom frame;
The sampling tube clamping mechanism is arranged on the first placing platform and used for driving the clamping plate mechanism to translate along the second avoiding groove so as to clamp or loosen the tube body of the sampling tube in a staggered manner;
the second working platform comprises a second linear motion mechanism and a cup separating operation mechanism, the second linear motion mechanism is fixed on the rack and drives the cup separating operation mechanism to move along the X axis, the cup separating operation mechanism comprises an objective table, a sample box fixing seat, a reagent tube fixing seat, a suction tube box fixing seat and a waste tube collecting bin, the sample box fixing seat, the reagent tube fixing seat, the suction tube box fixing seat and the waste tube collecting bin are all positioned on the objective table and are used for sequentially and correspondingly placing a sample box, at least one reagent tube, a suction tube box and waste suction tubes, the sample box is provided with a plurality of accommodating grooves, the reagent tubes are used for accommodating reaction reagents, and the suction tube box comprises a plurality of suction tubes with downward suction heads;
the spiral cover mechanism is positioned above the first working platform and comprises a third linear motion mechanism, a fourth linear motion mechanism and a spiral cover assembly, wherein the third linear motion mechanism is fixed on the frame and drives the fourth linear motion mechanism to move along the Y axis, the fourth linear motion mechanism drives the spiral cover assembly to move along the Z axis, and the spiral cover assembly is used for screwing the cover body of the sampling tube so as to separate or assemble the tube body and the cover body of the sampling tube;
The liquid suction mechanism is positioned above the second working platform and comprises a fifth linear motion mechanism and a second liquid taking component, wherein the fifth linear motion mechanism is fixed on the frame and drives the second liquid taking component to move along the Y axis, and the second liquid taking component is used for carrying a suction pipe to suck biological samples in sampling pipes or reaction reagents in reagent pipes, dripping the sucked biological samples or reaction reagents into a containing groove of the sample box and peeling waste suction pipes into a waste pipe collecting bin.
Preferably, the frame includes interconnect's lower casing and last casing, and first work platform, second work platform, spiral cover mechanism and imbibition mechanism all are built-in and are put the casing down, and a plurality of lower feet are installed to the diapire of lower casing, and display screen, scram button and first scanner are installed to the lateral wall, and first scanner is used for scanning the bar code on the sample box, and the last casing embeds has air filter mechanism.
Preferably, the second working platform further comprises a waste pipe guiding groove and a scraper, the waste pipe guiding groove is fixed on the frame and located below the waste pipe collecting bin and used for accommodating the suction pipe collected by the waste pipe collecting bin, and the scraper is fixed below the objective table and pushes the suction pipe in the waste pipe guiding groove to be discharged along with synchronous movement of the objective table.
Preferably, the sampling tube clamping mechanism comprises an eighth linear motion mechanism, a first guide rod, a wedge block, a second connecting seat, a third connecting seat, a first rotating shaft, a fourth connecting seat, a second guide rod, a first spring, a fifth connecting seat, a clamping hook, a sixth connecting seat, a third guide rod, a second spring, a sliding rail and a fixing boss, wherein the second connecting seat and the fourth connecting seat are connected with the first placing platform, the first guide rod is fixedly connected with the wedge block and is in sliding connection with the second connecting seat, the eighth linear motion mechanism drives the first guide rod to axially slide along the second connecting seat, one end of the second guide rod is a free end, the other end of the second guide rod is vertically connected with the fourth connecting seat, the fifth connecting seat is in sliding connection with the second guide rod, the first spring sleeve is arranged on the second guide rod, two ends of the first spring sleeve are respectively abutted against the fifth connecting seat and the fourth connecting seat, the third connecting seat is fixedly connected with the fifth connecting seat, the first rotating shaft is fixed on the third connecting seat and is connected with a bearing, the inclined surface of the wedge block is in contact with the bearing, the clamping hook is in two sliding connection with the third connecting seat, the sixth connecting seat is in sliding connection with the second connecting seat, the clamping seat is in sliding connection with the second clamping seat, the second connecting seat is in sliding connection with the third connecting seat is in the vertical connection with the clamping seat, the second connecting seat is in the clamping groove is in the vertical connection with the clamping groove, the clamping groove is in the sliding connection with the second connecting seat, or the second clamping seat is in the vertical connection with the fifth connecting seat is in the clamping groove, and the first connecting seat is in the vertical connection with the clamping seat, and the clamping groove is in the corresponding to the first connecting seat.
Preferably, the first placing platform comprises a first bottom plate which is horizontally arranged, and a first flange, a second flange and a first elastic reset mechanism which are all arranged on the first bottom plate, wherein the first bottom plate is connected with the first linear motion mechanism, the first flanges are two and mutually parallel and form a shape of with the second flange, a plurality of first spring plungers are respectively arranged on opposite sides of the two first flanges, a plurality of positioning bosses are arranged on the second flange, a plurality of first positioning grooves and a plurality of first positioning holes are formed in the side wall of the first bottom frame, the first positioning grooves are in fit limit with the positioning bosses, the first positioning holes are in fit limit with the first spring plungers, and at least one of the first elastic reset mechanism comprises a first fixing seat and at least one second spring plunger, and the clamping plate mechanism is propped against and reset to an initial state by the second spring plungers after being released.
Preferably, the clamping plate mechanism comprises a plurality of clamping plates, each clamping plate is sequentially arranged along the Z axis and is connected with the sixth connecting seat, a plurality of second through holes are formed in the clamping plates, the area where the second through holes of each clamping plate are located is sequentially arranged along the moving direction of the clamping plate mechanism, and the sum of the numbers of the second through holes is equal to the number of the first through holes of the first cover plate.
Preferably, the cap screwing mechanism further comprises a ranging sensor and a second scanner, the cap screwing assembly comprises a square frame, a second partition plate, a third partition plate and a cap screwing driving mechanism, the second partition plate and the third partition plate are horizontally arranged in the square frame, the ranging sensor is connected with the square frame and used for detecting whether a cap body of the sampling tube is unscrewed, the second scanner is connected with a fourth linear motion mechanism and used for scanning a bar code on the sampling tube, the cap screwing driving mechanism comprises a second motor, a first coupler, a key shaft sliding sleeve, a third spring, a key, a first pin shaft and clamping jaws, the second motor is connected with one end of the key shaft through the first coupler, the other end of the key shaft is axially and slidably connected with the key shaft sliding sleeve, the clamping jaws are sleeved on the key shaft sliding sleeve and vertically arranged in a penetrating mode through the first pin shaft, the second motor is fixed on the second partition plate, the key shaft sliding sleeve is slidably connected with the third partition plate, the third spring is sleeved outside the key shaft sliding sleeve and limited between the first pin shaft and the third partition plate, and when the cap is screwed, the cap screwing assembly is aligned with the sampling tube, and the clamping jaws are driven by the second motor to screw the cap body.
Preferably, the clamping jaw comprises a spiral cover head, a plurality of spiral cover clamp pages, a plurality of fourth springs, a plurality of second pin shafts and a plurality of first ejector pins, the spiral cover head is a two-section stepped shaft, the small end shaft section is sleeved in the key shaft sliding sleeve and vertically penetrates through the first pin shafts to be connected, the large end shaft section is annularly provided with a plurality of first through grooves, through holes perpendicular to the axis are formed in two sides of the first through grooves, the spiral cover clamp pages are in one-to-one correspondence with the first through grooves, the second pin shafts penetrating through the through holes are hinged, two ends of the fourth springs respectively correspond to the cover bodies which are propped against the space between the spiral cover clamp pages and the first through grooves and push the spiral cover clamp pages to rotate along the second pin shafts to clamp the sampling tubes, and the first ejector pins are symmetrically arranged on the large end side of the spiral cover head and the tip faces outwards.
Preferably, the second-stage liquid taking assembly comprises a sixth linear motion mechanism, a seventh linear motion mechanism, a suction pipe dismounting mechanism and a limiting plate, wherein:
the sixth linear motion mechanism is connected with the fifth linear motion mechanism and drives the seventh linear motion mechanism to move along the Z axis;
the seventh linear motion mechanism is connected with the sixth linear motion mechanism and drives the suction pipe dismounting mechanism to move along the Z axis;
the suction pipe disassembly and assembly mechanism comprises a ninth connecting seat, a plunger pump, a sleeve, a first guide seat, a second guide seat, a top plate, a disassembly mechanism, a fifth spring and a pressure sensor, wherein the ninth connecting seat is connected with the seventh linear motion mechanism;
And the limiting plate is connected with the fixed part of the sixth linear motion mechanism and abuts against the disassembly seat to limit when the straw disassembly and assembly mechanism moves upwards, so that the straw is quickly disassembled.
Preferably, each linear motion mechanism is a screw nut transmission mechanism or a synchronous belt transmission mechanism.
Compared with the prior art, the invention has the beneficial effects that:
1) The device integrates the functions of bar code scanning, cap screwing, liquid transferring and split charging and waste pipe recycling, can realize full-automatic split charging, reduce manual labor, reduce biological sample pollution and personnel secondary infection risk, greatly improve detection precision and efficiency, has compact structure, convenient operation, small size, convenient transportation and throwing use and wide application range, is especially suitable for occasions with high detection intensity and large flux, such as plate-type luminescence detection, plate-type enzyme immunity detection, PCR experiment pretreatment, mass spectrum detection, blood group detection, antibody screening and the like;
2) The clamping and positioning or loosening of the sampling tube are realized through the sampling tube operating mechanism, equipment damage or pollution caused by carrying the sampling tube by mistake in the clamping jaw back-off process after the screwing cover of the sampling tube is buckled is avoided, and the first placing platform, the sampling tube positioning mechanism and the sampling tube clamping mechanism are all detachably connected, so that the maintenance or the replacement is convenient;
3) The automatic peeling and imbibition of the suction pipe are realized through the secondary liquid taking component, the cooperation operation with each working platform is realized by utilizing the multi-stage motion, the machine is multifunctional, the structure is compact, the working procedure is simple, the whole machine body shape is further reduced, and the working efficiency is improved;
4) The scanner can rapidly and accurately acquire the corresponding information of the sampling tube and the sample box, and the pressure sensor is used for realizing the liquid level following when the suction tube absorbs liquid, so that the liquid absorption amount is ensured, the efficiency is high, and errors are not easy to occur;
5) By adopting the air filtering mechanism, the pollution risk of the biological sample can be reduced, and the detection precision of the biological sample and the heat dissipation capacity of the device can be improved.
Drawings
FIG. 1 is a schematic diagram of an automatic biological sample dispensing device according to the present invention;
FIG. 2 is a schematic diagram showing the internal structure of the automatic biological sample dispensing device according to the present invention;
FIG. 3 is a schematic view of a first working platform according to the present invention;
FIG. 4 is a schematic view of the sample tube handling mechanism of the present invention;
FIG. 5 is a schematic view of a sample tube positioning mechanism according to the present invention;
FIG. 6 is a schematic view of a first bottom frame structure according to the present invention;
FIG. 7 is a schematic view of a first clamping plate according to the present invention;
FIG. 8 is a schematic view of a second clamping plate according to the present invention;
FIG. 9 is a schematic view of a first separator plate according to the present invention;
FIG. 10 is a schematic view of a sample tube clamping mechanism of the present invention;
FIG. 11 is a schematic view of a partial structure of a sample tube clamping mechanism of the present invention;
FIG. 12 is a schematic view of a first view angle structure of a second work platform according to the present invention;
FIG. 13 is a schematic view of a second view angle structure of a second working platform according to the present invention;
FIG. 14 is a schematic view of a cap screwing mechanism according to the present invention;
FIG. 15 is a schematic view of a screw cap assembly according to the present invention;
FIG. 16 is a schematic view of a cap screwing driving mechanism according to the present invention;
FIG. 17 is a schematic view of a capping head according to the present invention;
FIG. 18 is a schematic view of a fifth linear motion mechanism according to the present invention;
FIG. 19 is a schematic view of a first view angle structure of a secondary liquid extraction module according to the present invention;
FIG. 20 is a schematic view of a second view angle structure of the secondary liquid extraction assembly of the present invention;
FIG. 21 is a schematic view of a suction pipe attaching and detaching mechanism according to the present invention;
fig. 22 is a schematic structural view of the dismounting mechanism of the present invention.
Reference numerals illustrate: 1. a frame; 2. a first work platform; 3. a second work platform; 4. a cap screwing mechanism; 5. a liquid suction mechanism; 6. a sampling tube; 7. a straw box; 8. a sample cartridge; 9. a reagent tube; 11. a lower housing; 11a, door panels; 12. a first scanner; 13. a foot margin; 14. a first notch; 15. a display screen; 16. an emergency stop button; 17. an upper housing; 18. an air filtration mechanism; 19. a shutter; 21. a first linear motion mechanism; 22. a sampling tube operating mechanism; 221. a first placement platform; 222. a sampling tube positioning mechanism; 223. a sampling tube clamping mechanism; 221a, a first bottom plate; 221b, a first flange; 221c, a first elastic return mechanism; 221d, a second flange; 222a, a first cover plate; 222b, a first bottom frame; 222c, a first clamping plate; 222d, a second clamping plate; 222e, a first separator; 222f, a first positioning groove; 222g, first positioning holes; 222h, a first avoiding groove; 222i, third through holes; 222j, blade slot; 222k, a second avoidance groove; 222l, handles; 223a, a first motor; 223b, a first lead screw nut mechanism; 223c, a first rail-slide mechanism; 223d, a first limiting mechanism; 223e, a first connection seat; 223f, first guide bar; 223g, wedge block; 223h, a second connecting seat; 223i, a third connecting seat; 223j, a first rotation shaft; 223k, fourth connecting seat; 223l, second guide bar; 223m, a first spring; 223n, fifth connecting seat; 223o, hook; 223p, sixth connecting seat; 223q, a third guide bar; 223r, a second spring; 223s, sliding rails; 223t, fixing the boss; 31. a second linear motion mechanism; 32. a cup separating operation mechanism; 33. a waste pipe guiding groove; 311. a first mounting plate; 312. a second rail-slider mechanism; 313. a first timing belt mechanism; 314. a tensioning mechanism; 315. a seventh connecting seat; 316. a second limiting mechanism; 314a, pulley holders; 314b, eighth connection base; 314c, tensioning screws; 321. a sample box fixing seat; 322. a reagent tube fixing seat; 323. a suction tube box fixing seat; 324. a waste pipe collecting bin; 325. an objective table; 326. a scraper; 41. a third linear motion mechanism; 42. a fourth linear motion mechanism; 43. a cap screwing assembly; 44. a ranging sensor; 45. a second scanner; 431. a block; 432. a second separator; 433. a third separator; 434. a cap screwing driving mechanism; 434a, a second motor; 434b, a first coupling; 434c, a key shaft; 434d, a key shaft sliding sleeve; 434e, a third spring; 434f, keys; 434g, first pin; 434h, capping head; 434i, a spiral cover clip; 434j, fourth spring; 434k, a second pin; 434l, first thimble; 51. a fifth linear motion mechanism; 52. a secondary liquid taking component; 521. a sixth linear motion mechanism; 522. a seventh linear motion mechanism; 523. a straw dismounting mechanism; 524. a limiting plate; 523a, a ninth connection base; 523b, a plunger pump; 523c, a pressure sensor; 523d, cannula; 523e, a first guide seat; 523f, a second guide seat; 523g, top plate; 523h, a dismounting mechanism; 523i, fifth springs; 523j, removing the seat; 523k, fourth guide bar; 523l, a third avoidance hole.
Detailed Description
The following description of the technical solutions in the embodiments of the present application will be made clearly and completely with reference to the drawings in the embodiments of the present application, and it is apparent that the described embodiments are only some embodiments of the present application, not all embodiments. All other embodiments, which can be made by one of ordinary skill in the art without undue burden from the present disclosure, are within the scope of the present disclosure.
It will be understood that when an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used herein in the description of the present application is for the purpose of describing particular embodiments only and is not intended to be limiting of the application.
In this application, referring to fig. 1, the side where the anchor 13 is located is taken as the lower side, the side where the display screen 15 is located is taken as the front, and the side where the first notch 14 is located is the right side, that is, the up-down direction corresponds to the Z axis, the left-right direction corresponds to the X axis, and the front-back direction corresponds to the Y axis. This orientation description is for reference only and is not intended to be limiting.
As shown in fig. 1-22, an automatic biological sample dispensing device comprising:
a frame 1;
first work platform 2, including first linear motion mechanism 21 and sampling tube operating mechanism 22, first linear motion mechanism 21 is fixed in frame 1 to drive sampling tube operating mechanism 22 carries out the X axle and removes, and sampling tube operating mechanism 22 includes first place platform 221, sampling tube positioning mechanism 222 and sampling tube clamping mechanism 223, wherein:
a first placement platform 221 connected to the first linear motion mechanism 21;
the sampling tube positioning mechanism 222 is mounted on the first placing platform 221, and comprises a first cover plate 222a, a clamping plate mechanism, a first bottom frame 222b and a first partition plate 222e, wherein the first cover plate 222a is connected with the first bottom frame 222b, the clamping plate mechanism and the first partition plate 222e are internally arranged under the first bottom frame 222b, a plurality of first through holes are formed in the first cover plate 222a, a plurality of second through holes are formed in the clamping plate mechanism, a plurality of third through holes 222i are formed in the first partition plate 222e, the first through holes, the second through holes and the third through holes are coaxially arranged and are sequentially distributed along the axial direction in an initial state, the sampling tube 6 is used for accommodating biological samples, a plurality of blade grooves 222j are formed in the inner side of the lower wall of the first bottom frame 222b, a plurality of blades are correspondingly distributed under the third through holes 222i and are internally arranged in the blade grooves 222j, a plurality of first avoidance grooves 222h corresponding to the blades are formed in the first partition plate 222e, and a second avoidance groove 222k is formed in the side wall of the first bottom frame 222 b;
The sampling tube clamping mechanism 223 is arranged on the first placing platform 221 and is used for driving the clamping plate mechanism to translate along the second avoiding groove 222k so as to clamp or unclamp the tube body of the sampling tube 6 in a staggered manner;
the second working platform 3 comprises a second linear motion mechanism 31 and a cup separating operation mechanism 32, the second linear motion mechanism 31 is fixed on the frame 1 and drives the cup separating operation mechanism 32 to move along an X axis, the cup separating operation mechanism 32 comprises a carrying table 325, a sample box fixing seat 321, a reagent tube fixing seat 322, a suction tube box fixing seat 323 and a waste tube collecting bin 324, the sample box fixing seat 321, the reagent tube fixing seat 322, the suction tube box fixing seat 323 and the waste tube collecting bin 324 are all positioned on the carrying table 325 and are used for sequentially and correspondingly placing a sample box 8, at least one reagent tube 9, a suction tube box 7 and waste suction tubes, the sample box 8 is provided with a plurality of accommodating grooves, the reagent tubes 9 are used for accommodating reaction reagents, and the suction tube box 7 comprises a plurality of suction tubes with downward suction heads;
the cap screwing mechanism 4 is positioned above the first working platform 2 and comprises a third linear motion mechanism 41, a fourth linear motion mechanism 42 and a cap screwing assembly 43, wherein the third linear motion mechanism 41 is fixed on the frame 1 and drives the fourth linear motion mechanism 42 to move along the Y axis, the cap screwing assembly 43 is driven by the fourth linear motion mechanism 42 to move along the Z axis, and the cap screwing assembly is used for screwing the cap body of the sampling tube 6, so that the separation or assembly of the tube body and the cap body of the sampling tube 6 is realized;
The liquid suction mechanism 5 is located above the second working platform 3, and comprises a fifth linear motion mechanism 51 and a second-stage liquid taking component 52, wherein the fifth linear motion mechanism 51 is fixed on the frame 1 and drives the second-stage liquid taking component 52 to move along the Y axis, and the second-stage liquid taking component 52 is used for carrying a suction pipe to suck biological samples or reaction reagents in the reagent pipe 9 in the sampling pipe 6, dripping the sucked biological samples or reaction reagents into a containing groove of the sample box 8, and peeling a waste suction pipe into the waste pipe collecting bin 324.
The sample box 8, the reagent tubes 9 and the pipette box 7 are all structures in the prior art, for example, the sample box 8 is a 96-well plate, each reagent tube 9 is used for accommodating a reaction reagent, for example, when the reagent is used for detecting nucleic acid, the reaction reagent can be proteinase K, etc., the reagents accommodated by each reagent tube 9 can be the same or different, the adjustment is carried out according to actual requirements, and the pipette box 7 corresponds to a box body in which 96 pipettes are placed. The sample box fixing seat 321, the reagent tube fixing seat 322, the straw box fixing seat 323 and the waste tube collecting bin 324 are sequentially arranged from front to back and can be in any shape, and in the embodiment, the sample box fixing seat 321 is a rectangular frame and is matched with the sample box 8 for limiting. The reagent pipe fixing base 322 is provided with a plurality of mounting holes side by side along the left-right direction, each reagent pipe 9 is placed with the mounting hole matching, the straw box fixing base 323 is including placing seat and four bracing pieces, place the seat and be the rectangle, four bracing piece one end is connected with the four corners of placing the seat, the other end is fixed on objective table 325, the middle part fretwork of placing the seat, straw box 7 hangs and stands on placing the seat, waste pipe collection bin 324 is rectangular bucket, but lower part butt joint recovery mechanism, and waste pipe collection bin 324 still can be the receiver directly.
In one embodiment, the rack 1 includes a lower housing 11 and an upper housing 17 that are connected to each other, the first working platform 2, the second working platform 3, the cap screwing mechanism 4 and the liquid sucking mechanism 5 are all built in the lower housing 11, a plurality of feet 13 are installed on the bottom wall of the lower housing 11, a display screen 15, a scram button 16 and a first scanner 12 are installed on the side wall, the first scanner 12 is used for scanning a bar code on the sample box 8, and an air filtering mechanism 18 is built in the upper housing 17.
In this embodiment, the upper housing 17 is located above the lower housing 11, and is a square housing. The lower shell 11 and the upper shell 17 are built by sectional materials, or can also adopt a welding mode, each surface forms a closed cavity through covering surfaces, the panel can be made of transparent materials, the observation is convenient, the front panel of the lower shell 11 is divided into an upper plate and a door plate 11a, the door plate 11a can be opened or closed, the taking and the maintenance are convenient, the first working platform 2, the second working platform 3, the spiral cover mechanism 4 and the liquid absorbing mechanism 5 are all arranged in the lower shell 11, the bottom wall of the lower shell 11 is provided with a plurality of ground feet 13, the adjustment level is convenient, the running stability is ensured, the ground feet 13 can be any number, preferably four, are respectively and correspondingly distributed at four corners, the side walls are provided with a display screen 15, an emergency stop button 16 and a first scanner 12, the first scanner 12 is used for scanning bar codes on the sample box 8, the portable electronic device can be fixed at any position, such as the first scanner 12 is installed on the inner side of the right wall of the lower shell 11, the display screen 15 is fixed on the upper plate of the front panel of the lower shell 11, the display screen 15 can be of any structure in the prior art, such as a USB interface can be integrated, operation and data storage are facilitated, the upper shell 17 is internally provided with an air filtering mechanism 18, the air filtering mechanism 18 is any filtering mechanism in the prior art, such as a filter screen and a fan, vent openings are formed in the lower shell 11 and the upper shell 17, the filter screen is installed on the vent openings, ventilation and heat dissipation are performed through the fan, air is filtered through the filter screen, the inside of each shell is always in a clean environment, pollution to biological samples is avoided, and improved detection precision is facilitated. The shielding plate 19 is further horizontally arranged on the upper side of the bottom wall of the lower shell 11, and the shielding plate 19 is provided with avoiding grooves corresponding to the first linear motion mechanism 21 and the second linear motion mechanism 31, so that dust prevention is facilitated, and the service life and the attractiveness of the equipment are improved. It should be noted that the frame 1 may also be any structure designed according to practical requirements.
In one embodiment, the second working platform 3 further comprises a waste pipe guiding groove 33 and a scraper 326, wherein the waste pipe guiding groove 33 is fixed on the frame 1 and is positioned below the waste pipe collecting bin 324 and is used for accommodating the suction pipe collected by the waste pipe collecting bin 324, and the scraper 326 is fixed below the stage 325 and moves synchronously with the stage 325 to push the suction pipe in the waste pipe guiding groove 33 to be discharged.
As shown in fig. 12 and 13, the waste pipe guiding groove 33 is fixed on the frame 1, for example, the cross section of the waste pipe guiding groove is U-shaped, and a slope is arranged on one side close to the frame 1, so that the waste pipe guiding groove is convenient to slide down. In this embodiment, the waste pipe guiding groove 33 is fixedly connected with the right wall of the frame 1, and the right wall of the lower housing 11 of the frame 1 is correspondingly provided with the first notch 14, when the scraper 326 moves synchronously with the stage 325, that is, when moving away from the direction of the first notch 14, the waste pipe guiding groove 33 is not blocked by the waste pipe, and when moving towards the direction of the first notch 14, the waste pipe guiding groove 33 is pushed to directly discharge the waste pipe from the first notch 14 to the outside of the device, so that the waste pipe is convenient to collect and carry, the operation is convenient, and the work efficiency is improved. It should be noted that, the structure or position of the waste pipe guiding groove 33 may be adjusted according to actual requirements, for example, the waste pipe may be placed obliquely, so that the waste pipe slides out under the action of its own weight.
In one embodiment, the sampling tube clamping mechanism 223 comprises an eighth linear motion mechanism, a first guide rod 223f, a wedge block 223g, a second connecting seat 223h, a third connecting seat 223i, a first rotating shaft 223j, a fourth connecting seat 223k, a second guide rod 223l, a first spring 223m, a fifth connecting seat 223n, a clamping hook 223o, a sixth connecting seat 223p, a third guide rod 223q, a second spring 223r, a sliding rail 223s and a fixing boss 223t, wherein the second connecting seat 223h and the fourth connecting seat 223k are connected with the first placing platform 221, the first guide rod 223f is fixedly connected with the wedge block 223g and is in sliding connection with the second connecting seat 223h, the eighth linear motion mechanism drives the first guide rod 223f to axially slide along the second connecting seat 223h, one end of the second guide rod 223l is a free end, the other end of the second guide rod is vertically connected with the fourth connecting seat 223k, the fifth connecting seat 223n is in sliding connection with the second guide rod 223l, the first spring 223m is sleeved on the second guide rod 223l, and both ends are respectively abutted against the fifth connecting seat 223n and the fourth connecting seat 223k, the third connecting seat 223i is fixedly connected with the fifth connecting seat 223n, the first rotating shaft 223j is fixed on the third connecting seat 223i and is connected with a bearing, the inclined surface of the wedge-shaped block 223g is contacted with the bearing, the clamping hook 223o is two and is in sliding connection with the third guide rod 223q, the sixth connecting seat 223p is connected with a clamping plate mechanism, the fixed boss 223t is vertically connected with the sixth connecting seat 223p, the third guide rod 223q is connected with the fixed boss 223t and is vertical to the second guide rod 223l, the second springs 223r are two and are sleeved on the third guide rod 223q, both ends of the second springs 223r are respectively abutted against the fixed boss 223t and one clamping hook 223o thereof, the clamping hook 223o is correspondingly and in clamping connection with the clamping groove, the eighth linear motion mechanism drives the wedge-shaped block 223g to move, thereby driving the third connecting seat 223i to drive the clamping plate mechanism to move, the dislocation clamping or loosening of the tube body of the sampling tube 6 is realized.
In an embodiment, the first placement platform 221 includes a first bottom plate 221a disposed horizontally, a first flange 221b, a second flange 221d and a first elastic reset mechanism 221c all mounted on the first bottom plate 221a, the first bottom plate 221a is connected with the first linear motion mechanism 21, the first flanges 221b are two and parallel to each other and form a shape of "with the second flange 221d, opposite sides of the two first flanges 221b are respectively provided with a plurality of first spring plungers, the second flange 221d is provided with a plurality of positioning bosses, a plurality of first positioning slots 222f and a plurality of first positioning holes 222g are formed in a side wall of the first bottom frame 222b, the first positioning slots 222f are matched and limited with the positioning bosses, the first positioning holes 222g are matched and limited with the first spring plungers, at least one of the first elastic reset mechanism 221c includes a first fixing seat and at least one second spring plunger, and the clamping plate mechanism is reset to an initial state by the second spring plungers after being released.
In an embodiment, the clamping plate mechanism includes a plurality of clamping plates, each clamping plate is sequentially disposed along the Z-axis and is connected to the sixth connecting seat 223p, a plurality of second through holes are formed on the clamping plate, the area where the second through holes of each clamping plate are located is sequentially disposed along the moving direction of the clamping plate mechanism, and the sum of the numbers of the second through holes is equal to the number of the first through holes of the first cover plate 222 a.
In this embodiment, the sampling tube clamping mechanism 223, the second flange 221d and the first elastic reset mechanism 221c are all located at the rear side of the sampling tube positioning mechanism 222, the front side of the sampling tube positioning mechanism 222 is provided with a handle 222l, which is convenient for drawing, two first flanges 221b are located at the left and right sides of the sampling tube positioning mechanism 222, and are located at adjacent sides of the sampling tube positioning mechanism 222, three first spring plungers are arranged side by side along the front and rear directions, three first positioning holes 222g are correspondingly formed in the side wall of the first bottom frame 222b, the first positioning holes 222g are matched with the first spring plungers to limit, three triangular positioning bosses are arranged side by side along the left and right directions on one side of the second flange 221d, which is close to the sampling tube positioning mechanism 222, and three first positioning grooves 222f are correspondingly formed in the side wall of the first bottom frame 222b, and are matched with the positioning bosses to limit, which is favorable for ensuring the positioning accuracy of the sampling tube positioning mechanism 222, and preventing lifting.
The two first elastic restoring mechanisms 221c are installed on the second flanges 221d side by side, the clamping plate mechanisms include a first clamping plate 222c and a second clamping plate 222d, the first clamping plate 222c, the second clamping plate 222d and the first partition plate 222e are sequentially arranged from top to bottom, if the sample box 8 is a 96-hole plate, 96 first through holes are formed in the corresponding first cover plate 222a, 96 third through holes are formed in the first partition plate 222e, 48 second through holes are formed in the first clamping plate 222c and the second clamping plate 222d respectively, the first clamping plate 222c and the second clamping plate 222d have the same or similar structures and are arranged in a staggered mode, in this embodiment, the first clamping plate 222c includes a first hole plate and a plurality of first reinforcing ribs, 48 second through holes are distributed on the first hole plate in an array mode, the two sides of each row (the front and back directions are in the Y-axis directions) of the hole positions are respectively provided with first reinforcing ribs, the rear sides of the first hole plate are connected with the sixth connecting seat 223p through screws, the two corners are provided with movement avoidance grooves, and the first reinforcing ribs extend to the first through holes corresponding to the first through holes, namely the first through holes are coaxial through holes and the first through holes are coaxial and extend to the first through holes and the first through holes are corresponding to the first through holes and the first through holes are coaxial through holes and the first through holes are in the first through holes and have initial conditions extend first through holes and first through holes are respectively first through holes. The second clamping plate 222d comprises a second orifice plate and a plurality of second reinforcing ribs, 48 second through holes are distributed on the second orifice plate in an array mode, the second reinforcing ribs are respectively arranged on two sides of each row of holes, in order to provide a movement avoidance space, the rear ends of the other second reinforcing ribs extend to an initial state when the front ends of the second orifice plates abut against the inner side of the front wall of the first bottom frame 222b, the rear ends of the second reinforcing ribs are vertically connected with connecting plates, the connecting plates are connected with sixth connecting seats 223p through screws, the fact that the first orifice plate and the second orifice plate are distributed in a vertically staggered mode is achieved, the first through holes, the second through holes and the third through holes are in one-to-one correspondence, and two second spring plungers are arranged on the first elastic reset mechanisms 221c and abut against the first clamping plate 222c and the second clamping plate 222d respectively. When the tube body of the sampling tube 6 needs to be clamped, the sampling tube clamping mechanism 223 drives the clamping plate mechanism to move forward, and when the clamping plate mechanism is loosened, the motor is reversed and automatically reset to the initial state under the action of the first elastic reset mechanism 221 c. It should be noted that, the positioning boss, the first elastic reset mechanism 221c, the first spring plunger and the second spring plunger may be any number, and the clamping plate mechanism may be further split into any number of clamping plates, so that when the clamping plates are adopted to clamp, the accumulated error is reduced, and a stable clamping force is ensured.
The second connection seat 223h, the first spring 223m, the fifth connection seat 223n, the second guide rod 223l, the hook 223o and the second spring 223r are two, which helps to ensure stability. The first rotating shaft 223j is vertically fixed on the third connecting seat 223i, the lower end of the first rotating shaft is connected with a bearing, the inclined surface of the wedge-shaped block 223g is in contact with the bearing, sliding friction is changed into rolling friction through the bearing, friction force is reduced, and the service life of equipment is prolonged. The handles and the sliding grooves are further connected to the clamping hooks 223o, sliding rails 223s are further arranged on the rear side of the sixth connecting seat 223p, the clamping hooks 223o are guided through the matching of the sliding grooves and the sliding rails 223s, and limiting bosses are further arranged at the two ends of the sliding rails 223s to prevent the clamping hooks 223o from slipping. The engagement connection between the hook 223o and the third connection seat 223i may be any engagement structure in the prior art. The first spring plunger is matched with the first bottom plate 221a for positioning, and the clamping hook 223o is in clamping connection with the third connecting seat 223i, so that the quick assembly disassembly is convenient for replacement or maintenance. The clamping positioning or loosening of the sampling tube is realized through the sampling tube operating mechanism, equipment damage or pollution caused by mistakenly carrying the sampling tube in the clamping jaw retreating process after the screwing cover of the sampling tube is prevented, and the first placing platform, the sampling tube positioning mechanism and the sampling tube clamping mechanism are detachably connected, so that the sampling tube is convenient to maintain or replace.
In an embodiment, the cap screwing mechanism 4 further includes a ranging sensor 44 and a second scanner 45, the cap screwing assembly 43 includes a frame 431, a second partition 432, a third partition 433 and a cap screwing driving mechanism 434, the second partition 432 and the third partition 433 are horizontally disposed in the frame 431, the ranging sensor 44 is connected with the frame 431 and is used for detecting whether the cap body of the sampling tube 6 is unscrewed, the second scanner 45 is connected with the fourth linear motion mechanism 42 and is used for scanning the bar code on the sampling tube 6, the cap screwing driving mechanism 434 includes a second motor 434a, a first coupler 434b, a key shaft 434c, a key shaft sliding sleeve 434d, a third spring 434e, a key 434f, a first pin shaft 434g and a clamping jaw, the second motor 434a is connected with one end of the key shaft 434c through the first coupler 434b, the other end of the key shaft 434c is axially and slidably connected with the key shaft sliding sleeve 434d, the clamping jaw is sleeved on the key shaft sliding sleeve 434d and is vertically and penetratingly connected with the second partition 434a through the first pin shaft 434g, the second motor 434a is fixed on the second partition 434a, the third partition 434d is screwed with the third partition 433 and is screwed with the cap body of the third partition 433 b and is located outside the cap body of the sampling tube 6 when the cap screwing assembly is screwed with the third partition 434 b.
In an embodiment, the clamping jaw includes a rotating cover head 434h, a plurality of rotating cover clamping pages 434i, a plurality of fourth springs 434j, a plurality of second pin shafts 434k and a plurality of first thimble 434l, the rotating cover head 434h is a two-section stepped shaft, the small end shaft section is sleeved in the key shaft sliding sleeve 434d and vertically penetrates through the first pin shaft 434g to be connected, the large end shaft section is annularly provided with a plurality of first through grooves, two sides of the first through grooves are provided with through holes perpendicular to the axis, the rotating cover clamping pages 434i are in one-to-one correspondence with the first through grooves and are hinged by a second pin shaft 434k penetrating through the through holes, two ends of the fourth springs 434j respectively correspond to and abut against the space between the rotating cover clamping pages 434i and the first through grooves and push the rotating cover clamping pages 434i to rotate along the second pin shafts 434k so as to clamp the cover body of the sampling tube 6, and the first thimble 434l is symmetrically arranged on the large end side of the rotating cover head 434h, and the tip faces outwards.
14-17, the opening of the square frame 431 of the cap screwing assembly 43 is downward, and the small end shaft section of the cap screwing head 434h is sleeved in the key shaft sliding sleeve 434d and is perpendicularly and axially connected in a penetrating manner through the first pin shaft 434 g. The key shaft 434c and the key shaft sliding sleeve 434d realize axial sliding connection through the key 434f, the number of the keys 434f can be adjusted according to actual requirements, and it should be noted that the key shaft 434c and the key shaft sliding sleeve 434d can also be respectively a spline shaft and a spline shaft sleeve to realize rotation limit. Four first through grooves are formed in the large end shaft section of the cap screwing head 434h in the circumferential direction, the cap screwing clamp page 434i is installed in the first through grooves and can rotate around the second pin shaft 434k, a chamfer is formed in the inner side of the lower end of the cap screwing clamp page 434i, a cover body of the sampling tube 6 is convenient to plug in, and a plurality of grooves are formed in the inner side to increase friction, so that the cover body is convenient to rotate. In the initial state, the lower end of the cap-rotating clamp 434i is inclined inward by the fourth spring 434j to help clamp the cap of the sampling tube 6.
When the cap is screwed, the third linear motion mechanism 41 and the fourth linear motion mechanism 42 drive the cap screwing assembly 43 to align the sampling tube 6, the cap screwing driving mechanism 434 of the cap screwing assembly 43 aligns the sampling tube 6 to press down, and buffer is carried out through the third spring 434e, so that the blades are inserted into the bottom of the tube body of the sampling tube 6 to stop rotating, the number of the blades can be any, for example, two blades which are arranged in parallel can avoid the crushing of the sampling tube 6 and overflow biological samples, the clamping jaw of the cap screwing driving mechanism 434 clamps the cap body of the sampling tube 6, namely, each cap screwing clamping page 434i clamps the cap body of the sampling tube 6 under the action of the fourth spring 434j, the first thimble 434l is inserted into the cap body of the sampling tube 6 to stop rotating, the second motor 434a drives the clamping jaw to rotate to open the cap body of the sampling tube 6, and the cap screwing assembly 43 carrying the cap body is driven by the fourth linear motion mechanism 42 to move upwards. After the liquid is taken out, the fourth linear motion mechanism 42 drives the cap screwing assembly 43 carrying the cap to move downwards, the second motor 434a rotates reversely to screw the cap of the sampling tube 6, the fourth linear motion mechanism 42 moves upwards and is driven by the third linear motion mechanism 41 to enable the cap screwing assembly 43 to operate on the next sampling tube 6, and liquid taking of all the sampling tubes 6 is completed in a circulating mode in sequence.
In one embodiment, the secondary liquid taking assembly 52 includes a sixth linear motion mechanism 521, a seventh linear motion mechanism 522, a straw removing mechanism 523, and a limiting plate 524, wherein:
a sixth linear motion mechanism 521 connected to the fifth linear motion mechanism 51 and driving the seventh linear motion mechanism 522 to perform Z-axis movement;
a seventh linear motion mechanism 522 connected to the sixth linear motion mechanism 521 and driving the suction pipe attaching/detaching mechanism 523 to perform a Z-axis movement;
the suction pipe disassembly and assembly mechanism 523 comprises a ninth connecting seat 523a, a plunger pump 523b, a sleeve 523d, a first guide seat 523e, a second guide seat 523f, a top plate 523g, a disassembly mechanism 523h, a fifth spring 523i and a pressure sensor, wherein the ninth connecting seat 523a is connected with the seventh linear motion mechanism 522, the plunger pump 523b, the first guide seat 523e and the second guide seat 523f are connected with the ninth connecting seat 523a, the disassembly mechanism 523h comprises a disassembly seat 523j and at least one fourth guide rod 523k which are mutually connected, a third avoidance hole 523l is formed in the disassembly seat 523j, the sleeve 523d penetrates through the third avoidance hole 523l and is parallel to the fourth guide rod 523k, one end of the sleeve 523d is connected with the plunger pump 523b for ventilation, the other end of the sleeve 523d is used for plugging the suction pipe, the pressure sensor 523c is used for detecting the air pressure of the sleeve 523d, the fourth guide rod 523e is respectively connected with the first guide seat 523e and the second guide seat 523f in a sliding manner, the disassembly seat 523j is limited between the first guide seat 523e and the second guide seat 523f, the fifth spring 523j is arranged on one end of the fourth guide seat 523j, and the fourth guide seat 523j is far away from the second guide seat 523f, and the fourth guide seat 523j is arranged on one end of the fourth guide seat 523j is far away from the fourth guide seat 523j, and is arranged on the other end, and is fixedly connected with the other end, and is arranged on the fourth guide seat 523j, and is correspondingly, and is connected with the fourth guide seat is;
The limiting plate 524 is connected to the fixing portion of the sixth linear movement mechanism 521, and abuts against the detaching seat 523j to limit when the suction pipe attaching/detaching mechanism 523 moves upward, thereby realizing quick detachment of the suction pipe.
The fifth linear motion mechanism 51 drives the sixth linear motion mechanism 521 to perform Y-axis motion, the sixth linear motion mechanism 521 drives the seventh linear motion mechanism 522 to perform first-stage Z-axis motion, the seventh linear motion mechanism 522 drives the straw disassembling and assembling mechanism 523 to perform second-stage Z-axis motion, and the cooperation with each working platform is realized through multistage motion. The limiting plate 524 is connected with the fixing portion of the sixth linear movement mechanism 521, and abuts against the disassembling seat 523j to limit when the straw disassembling mechanism 523 moves upwards, and in the process that the seventh linear movement mechanism 522 carries the ninth connecting seat 523a to move upwards, quick disassembly of the straw is realized through the blocking effect of the top plate 523g, an additional power mechanism is not needed, and multiple functions can be realized. And the suction pipe disassembly and assembly mechanism 523 is high in efficiency and not prone to error due to the fact that liquid level following is achieved through the pressure sensor in the liquid suction process. The number of the fourth guide rods 523k may be two, and the detachable seat 523j and the stopper 524 may be any number.
In one embodiment, each linear motion mechanism is a screw nut transmission mechanism or a synchronous belt transmission mechanism.
The screw-nut transmission mechanisms are similar in structure, such as the fourth linear motion mechanism 42, the seventh linear motion mechanism 522 and the eighth linear motion mechanism are screw-nut transmission mechanisms in this embodiment, where the eighth linear motion mechanism includes a first motor 223a, a first screw-nut mechanism 223b, a first rail-slider mechanism 223c and a first connecting seat 223e, the first connecting seat 223e is fixedly connected with a nut of the first screw-nut mechanism 223b and a slider of the first rail-slider mechanism 223c, the first guide rod 223f is connected with the first connecting seat 223e, the first motor 223a is fixed on the first bottom plate 221a through the motor connecting seat, and drives the first screw-nut mechanism 223b to move, so as to drive the first guide rod 223f to move, and the first rail-slider mechanism 223c may include one or more parallel rail-sliders, such as one, for guiding the first connecting seat 223 e. Further, the eighth linear motion mechanism further includes a first limiting mechanism 223d, where the first limiting mechanism 223d includes a plurality of limit switches for detecting a stroke and improving safety, which are well known to those skilled in the art and will not be described herein. The screw nut transmission mechanisms are similar in structure and can be adjusted according to actual requirements.
Each of the synchronous belt transmission mechanisms has a similar structure, such as the first linear motion mechanism 21, the second linear motion mechanism 31, the third linear motion mechanism 41, the fifth linear motion mechanism 51 and the sixth linear motion mechanism 521 are synchronous belt transmission mechanisms, wherein the second linear motion mechanism 31 includes a first mounting plate 311, a second guide rail slider mechanism 312, a first synchronous belt mechanism 313 and a seventh connecting seat 315, the first mounting plate 311 is fixed on the bottom wall of the frame 1, the first synchronous belt mechanism 313 is fixed on the first mounting plate 311, the seventh connecting seat 315 is fixedly connected with the stage 325, the synchronous belt of the first synchronous belt mechanism 313 and the slider of the second guide rail slider mechanism 312, the specific shape can be designed according to the actual requirement, the lower side of the seventh connecting seat 315 is connected with the stage by two rectangular blocks, the upper side is connected with the stage by two rectangular plates distributed vertically side by side, the second guide rail slider mechanism 312 can include one or more parallel guide rail sliders, such as two, and are arranged on two sides of the synchronous belt for guiding the seventh connecting seat 315. Further, the second linear motion mechanism 31 further includes a second limiting mechanism 316 and a tensioning mechanism 314, and the second limiting mechanism 316 includes a plurality of limiting switches for detecting a stroke and improving safety, which are well known to those skilled in the art and will not be described herein. The tensioning mechanism 314 includes an eighth connecting seat 314b and a tensioning screw 314c, the eighth connecting seat 314b is fixed on the first mounting plate 311, the tensioning screw 314c is in threaded connection with the eighth connecting seat 314b and the pulley fixing seat 314a of the first synchronous belt mechanism 313, and the tensioning of the first synchronous belt mechanism 313 is achieved by adjusting the tensioning screw 314c, which is a well known technology of those skilled in the art, and is not described herein, or any tensioning structure in the prior art can be adopted. Each synchronous belt transmission mechanism has a similar structure and can be adjusted according to actual requirements.
It should be noted that each linear motion mechanism may also be a structure for realizing linear motion in the prior art, such as a rack and pinion.
The device integrates the functions of bar code scanning, cap screwing, liquid transferring and split charging and waste pipe recycling, can realize full-automatic split charging, reduce manual labor, reduce biological sample pollution and personnel secondary infection risk, greatly improve detection precision and efficiency, has compact structure, convenient operation, small size, convenient transportation and throwing use and wide application range, is especially suitable for occasions with high detection intensity and large flux, such as plate-type luminescence detection, plate-type enzyme immunity detection, PCR experiment pretreatment, mass spectrum detection, blood group detection, antibody screening and the like; the clamping and positioning or loosening of the sampling tube are realized through the sampling tube operating mechanism, equipment damage or pollution caused by carrying the sampling tube by mistake in the clamping jaw back-off process after the screwing cover of the sampling tube is buckled is avoided, and the first placing platform, the sampling tube positioning mechanism and the sampling tube clamping mechanism are all detachably connected, so that the maintenance or the replacement is convenient; the automatic peeling and imbibition of the suction pipe are realized through the secondary liquid taking component, the cooperation operation with each working platform is realized through the multi-stage motion, the one-machine multi-function is realized, the structure is compact, the working procedure is simple, the whole machine body shape is further reduced, and the working efficiency is improved; the scanner can rapidly and accurately acquire the corresponding information of the sampling tube and the sample box, and the pressure sensor is used for realizing the following of the liquid level when the suction tube absorbs liquid, so that the efficiency is high and the error is not easy to occur; by adopting the air filtering mechanism, the pollution risk of the biological sample can be reduced, and the detection precision of the biological sample and the heat dissipation capacity of the device can be improved.
The working principle of the device is as follows:
the sampling tube 6 containing the biological sample is placed in the hole site corresponding to the sampling tube positioning mechanism 222, the bar code is attached to each sampling tube 6, the sample box 8 is placed on the sample box fixing seat 321, the bar code is attached to the sample box 8, the side where the bar code is located is aligned with the first scanner 12, each reagent tube 9 containing the reaction reagent is placed in the hole site corresponding to the reagent tube fixing seat 322, and the pipette box 7 is placed on the pipette box fixing seat 323.
The first scanner 12 scans the bar code on the sample box 8 by power-on initialization, the first linear motion mechanism 21 drives the sample tube operating mechanism 22 to move right, meanwhile, the third linear motion mechanism 41 and the fourth linear motion mechanism 42 drive the spiral cover assembly 43 to move, so that the spiral cover assembly 43 aligns with the sample tube 6, for example, after aligning with the first sample tube 6 at the left front, the fourth linear motion mechanism 42 drives the spiral cover assembly 43 to move downwards, after clamping the sample tube 6, the whole sample tube 6 is carried to move upwards, and after aligning with the second scanner 45, the spiral cover assembly 43 drives the sample tube 6 to rotate to scan the bar code. After the scanning is completed, the cap screwing assembly 43 drives the sampling tube 6 to move downwards, the ranging sensor 44 detects that the cap of the sampling tube 6 is not unscrewed, the cap screwing driving mechanism 434 is used for pressing the sampling tube 6 downwards, the third spring 434e is used for buffering, the blade is inserted into the bottom of the tube of the sampling tube 6 to stop rotating, the clamping jaw of the cap screwing driving mechanism 434 clamps the cap of the sampling tube 6, namely, each cap screwing clamping page 434i clamps the cap of the sampling tube 6 under the action of the fourth spring 434j, the first thimble 434l is inserted into the cap of the sampling tube 6 to stop rotating, the second motor 434a is used for driving the clamping jaw to rotate to screw the cap to open the cap of the sampling tube 6, and the fourth linear movement mechanism 42 is used for driving the cap screwing assembly 43 carrying the cap to move upwards.
Meanwhile, the fifth linear motion mechanism 51 drives the secondary liquid taking component 52 to move, so that the sleeve 523d is aligned with the suction pipe in the suction pipe box 7, for example, after being aligned with the first suction pipe at the left front, the sixth linear motion mechanism 521 drives the seventh linear motion mechanism 522 to carry the sleeve 523d to move downwards, the plunger pump 523b works to absorb and butt the suction pipe, the sixth linear motion mechanism 521 drives the seventh linear motion mechanism 522 to carry the suction pipe to move upwards, the second linear motion mechanism 31 drives the cup separating operation mechanism 32 to move rightwards, meanwhile, the first linear motion mechanism 21 drives the sampling pipe operation mechanism 22 to move rightwards, the fifth linear motion mechanism 51 drives the secondary liquid taking component 52 to move to align with the sampling pipe 6 after the cover, the seventh linear motion mechanism 522 drives the suction pipe dismounting mechanism 523 to move downwards to absorb the biological sample in the sampling pipe 6, and the pressure sensor 523c is used for detecting the air pressure of the sleeve 523d to realize liquid suction and liquid level following in the liquid suction process, so as to guarantee liquid suction amount. After the liquid suction is completed, the seventh linear motion mechanism 522 drives the straw disassembling and assembling mechanism 523 to move upwards, then the first linear motion mechanism 21 drives the sampling tube operating mechanism 22 to move leftwards, the second linear motion mechanism 31 drives the cup separating operating mechanism 32 to move leftwards, the fourth linear motion mechanism 42 drives the cap screwing assembly 43 carrying the cap body to move downwards, the ranging sensor 44 detects that the cap body of the sampling tube 6 is unscrewed, the eighth linear motion mechanism of the sampling tube clamping mechanism 223 drives the clamping plate mechanism to translate so as to clamp the tube body of the sampling tube 6 in a staggered manner, the second motor 434a reversely rotates to screw the cap body of the sampling tube 6, the fourth linear motion mechanism 42 moves upwards, and the eighth linear motion mechanism drives the clamping plate mechanism to translate so as to release and reset the tube body of the sampling tube 6 to an initial state. Then, the fourth linear motion mechanism 42 is driven by the third linear motion mechanism 41 to enable the cap screwing assembly 43 to operate on the next sampling tube 6, and liquid sampling of all the sampling tubes 6 is completed in a circulating mode.
The fifth linear motion mechanism 51 drives the second-stage liquid taking component 52 to move forward to align with the first containing groove at the left front side in the sample box 8, the sixth linear motion mechanism 521 drives the seventh linear motion mechanism 522 to move downwards with the suction pipe, the plunger pump 523b works to drop the biological sample sucked by the suction pipe into the containing groove, then the sixth linear motion mechanism 521 drives the seventh linear motion mechanism 522 to move upwards with the suction pipe, the fifth linear motion mechanism 51 drives the second-stage liquid taking component 52 to move backwards to align with the waste pipe collecting bin 324, and the limiting plate 524 limits by abutting against the detaching seat 523j under the driving of the seventh linear motion mechanism 522 and realizes the quick detachment of the suction pipe under the blocking effect of the top plate 523 g.
The waste pipe collecting bin 324 collects waste straws, and when the scraping plate 326 moves synchronously with the objective table 325, namely when moving away from the direction of the first notch 14, the waste pipe collecting bin leaves a containing space for facilitating the waste straws to slide into the waste pipe guiding groove 33 without blocking, and when moving towards the direction of the first notch 14, the waste pipe guiding groove 33 is pushed to directly discharge the straws from the first notch 14 to the outside of the device, so that the waste straws can be collected and carried conveniently.
The fifth linear motion mechanism 51 drives the secondary liquid taking component 52 to move forward, so that the sleeve 523d is aligned with the suction pipe in the suction pipe box 7, for example, after being aligned with the second suction pipe at the left front, the sixth linear motion mechanism 521 drives the seventh linear motion mechanism 522 to carry the sleeve 523d to move downward, the plunger pump 523b works to absorb and butt the suction pipe, the sixth linear motion mechanism 521 drives the seventh linear motion mechanism 522 to carry the suction pipe to move upward, the fifth linear motion mechanism 51 drives the secondary liquid taking component 52 to move forward and be aligned with the first reagent pipe 9 at the left, the sixth linear motion mechanism 521 drives the seventh linear motion mechanism 522 to carry the sleeve 523d to move downward, the reaction reagent in the reagent pipe 9 is sucked, and the pressure sensor 523c is used for detecting the air pressure of the sleeve 523d to realize liquid level following while liquid suction in the liquid suction process, so as to ensure the liquid suction amount. After the liquid suction is completed, the seventh linear motion mechanism 522 drives the straw disassembling and assembling mechanism 523 to move upwards, the fifth linear motion mechanism 51 drives the secondary liquid taking component 52 to move forwards to align with the first containing groove at the left front side in the sample box 8, the sixth linear motion mechanism 521 drives the seventh linear motion mechanism 522 to move downwards with the straw, the plunger pump 523b works to drop the biological sample sucked by the straw into the containing groove, then the sixth linear motion mechanism 521 drives the seventh linear motion mechanism 522 to move upwards with the straw, the fifth linear motion mechanism 51 drives the secondary liquid taking component 52 to move backwards to align with the waste pipe collecting bin 324, and the limiting plate 524 is limited by abutting against the disassembling seat 523j under the driving of the seventh linear motion mechanism 522 and realizes the quick disassembly of the straw under the blocking effect of the top plate 523 g. The pipetting mechanism 5 then continues to aspirate the next pipette cycle, each holding tank being adapted to hold a biological sample and a corresponding reagent in one of the sampling tubes 6, the number of aspirates being adjustable according to the type of reagent. The specific steps can be adjusted according to actual demands, for example, each straw can also absorb the reaction reagent and the biological sample at one time and then inject the reaction reagent and the biological sample into the accommodating groove together so as to further improve the efficiency.
The technical features of the above-described embodiments may be arbitrarily combined, and all possible combinations of the technical features in the above-described embodiments are not described for brevity of description, however, as long as there is no contradiction between the combinations of the technical features, they should be considered as the scope of the description.
The above-described embodiments are merely representative of the more specific and detailed embodiments described herein and are not to be construed as limiting the scope of the claims. It should be noted that it would be apparent to those skilled in the art that various modifications and improvements could be made without departing from the spirit of the present application, which would be within the scope of the present application. Accordingly, the scope of protection of the present application is to be determined by the claims appended hereto.
Claims (10)
1. An automatic partial shipment device of biological sample, its characterized in that: the automatic split charging device for biological samples comprises:
a frame (1);
first work platform (2), including first linear motion mechanism (21) and sampling tube operating device (22), first linear motion mechanism (21) is fixed in on frame (1), and drive sampling tube operating device (22) carry out X axle and remove, sampling tube operating device (22) include first place platform (221), sampling tube positioning device (222) and sampling tube clamping device (223), wherein:
The first placing platform (221) is connected with the first linear motion mechanism (21);
the sampling tube positioning mechanism (222) is mounted on the first placing platform (221), and comprises a first cover plate (222 a), a clamping plate mechanism, a first bottom frame (222 b) and a first partition plate (222 e), wherein the first cover plate (222 a) is connected with the first bottom frame (222 b), the clamping plate mechanism and the first partition plate (222 e) are internally arranged in the first bottom frame (222 b), a plurality of first through holes are formed in the first cover plate (222 a), a plurality of second through holes are formed in the clamping plate mechanism, a plurality of third through holes (222 i) are formed in the first partition plate (222 e), the first through holes, the second through holes and the third through holes are coaxially arranged and are sequentially distributed along the axial direction, and are used for placing a sampling tube (6), a plurality of blade grooves (222 j) are formed in the inner side of the lower wall of the first bottom frame (222 b), the blade grooves (222 j) are correspondingly distributed below the first through holes (222 e), and the first blade grooves (222 h) are formed in the first partition plate (222 e), and the first side walls (222 h) are correspondingly formed in the first side walls;
The sampling tube clamping mechanism (223) is arranged on the first placing platform (221) and is used for driving the clamping plate mechanism to translate along the second avoiding groove (222 k), so that the tube body of the sampling tube (6) is clamped or loosened through the radial movement of the second through hole relative to the first through hole or the third through hole;
the second working platform (3) comprises a second linear motion mechanism (31) and a cup separating operation mechanism (32), wherein the second linear motion mechanism (31) is fixed on the rack (1) and drives the cup separating operation mechanism (32) to move along an X axis, the cup separating operation mechanism (32) comprises an objective table (325), a sample box fixing seat (321), a reagent tube fixing seat (322), a suction tube box fixing seat (323) and a waste tube collecting bin (324), the sample box fixing seat (321), the reagent tube fixing seat (322), the suction tube box fixing seat (323) and the waste tube collecting bin (324) are all positioned on the objective table (325) and are used for sequentially and correspondingly placing a sample box (8), at least one reagent tube (9), a suction tube box (7) and waste suction tubes, the suction tube box (8) is provided with a plurality of accommodating grooves, the reagent tube (9) is used for accommodating reaction reagents, and the suction tube box (7) comprises a plurality of downward suction tubes;
The cap screwing mechanism (4) is positioned above the first working platform (2) and comprises a third linear motion mechanism (41), a fourth linear motion mechanism (42) and a cap screwing assembly (43), wherein the third linear motion mechanism (41) is fixed on the frame (1) and drives the fourth linear motion mechanism (42) to move along the Y axis, the cap screwing assembly (43) is driven by the fourth linear motion mechanism (42) to move along the Z axis, and the cap screwing assembly is used for screwing the cap body of the sampling tube (6) to separate or assemble the tube body and the cap body of the sampling tube (6);
the liquid suction mechanism (5) is located above the second working platform (3), and comprises a fifth linear motion mechanism (51) and a second-stage liquid taking component (52), wherein the fifth linear motion mechanism (51) is fixed on the frame (1) and drives the second-stage liquid taking component (52) to move along a Y axis, and the second-stage liquid taking component (52) is used for carrying a straw to suck biological samples in the sampling pipe (6) or reaction reagents in the reagent pipe (9), dripping the sucked biological samples or reaction reagents into a containing groove of the sample box (8) and peeling the waste straw into the waste pipe collecting bin (324).
2. The automated biological sample dispensing apparatus of claim 1, wherein: frame (1) are including interconnect's lower casing (11) and last casing (17), first work platform (2), second work platform (3), spiral cover mechanism (4) and imbibition mechanism (5) are all built-in lower casing (11), a plurality of lower margin (13) are installed to the diapire of lower casing (11), and display screen (15), scram button (16) and first scanner (12) are installed to the lateral wall, first scanner (12) are used for the scanning bar code on sample box (8), upward casing (17) are built-in have air filter mechanism (18).
3. The automated biological sample dispensing apparatus of claim 1, wherein: the second working platform (3) further comprises a waste pipe guiding groove (33) and a scraping plate (326), wherein the waste pipe guiding groove (33) is fixed on the frame (1) and located below the waste pipe collecting bin (324) and used for accommodating the suction pipes collected by the waste pipe collecting bin (324), and the scraping plate (326) is fixed below the objective table (325) and pushes the suction pipes in the waste pipe guiding groove (33) to be discharged along with synchronous movement of the objective table (325).
4. The automated biological sample dispensing apparatus of claim 1, wherein: the sampling tube clamping mechanism (223) comprises an eighth linear motion mechanism, a first guide rod (223 f), a wedge block (223 g), a second connecting seat (223 h), a third connecting seat (223 i), a first rotating shaft (223 j), a fourth connecting seat (223 k), a second guide rod (223 l), a first spring (223 m), a fifth connecting seat (223 n), a clamping hook (223 o), a sixth connecting seat (223 p), a third guide rod (223 q), a second spring (223 r), a sliding rail (223 s) and a fixing boss (223 t), wherein the second connecting seat (223 h) and the fourth connecting seat (223 k) are connected with the first placing platform (221), the first guide rod (223 f) is fixedly connected with the wedge block (223 g) and is in sliding connection with the second connecting seat (223 h), the eighth linear motion mechanism drives the first guide rod (223 f) to axially slide along the second connecting seat (223 h), one end of the guide rod (223 l) is a free end, the other end of the second guide rod (223 l) is vertically connected with the fifth connecting seat (223 l), the fourth guide rod (223 k) is fixedly connected with the fifth connecting seat (223 l) and the fifth connecting seat (223 l) is fixedly connected with the fifth connecting seat (223 i) by the fifth connecting seat (223 i), the first rotating shaft (223 j) is fixed on the third connecting seat (223 i) and is connected with a bearing, the inclined surface of the wedge-shaped block (223 g) is in contact with the bearing, two clamping hooks (223 o) are in sliding connection with the third guide rod (223 q), the sixth connecting seat (223 p) is connected with a clamping plate mechanism, a fixing boss (223 t) is vertically connected with the sixth connecting seat (223 p), the third guide rod (223 q) is connected with the fixing boss (223 t) and is vertical to the second guide rod (223 l), two second springs (223 r) are respectively sleeved on the third guide rod (223 q), two ends of each second spring (223 r) are respectively abutted to the fixing boss (223 t) and one clamping hook (223 o), the third connecting seat (223 i) is provided with a clamping groove, the clamping hooks (223 o) are correspondingly clamped and connected with the clamping groove, and the eighth linear motion mechanism drives the wedge-shaped block (223 g) to drive the third connecting seat (223 i) to move, so that the wedge-shaped block (223 i) can be loosened.
5. The automated biological sample dispensing apparatus of claim 4, wherein: the first placement platform (221) comprises a first bottom plate (221 a) which is horizontally arranged, a first flange (221 b), a second flange (221 d) and a first elastic reset mechanism (221 c), wherein the first flange (221 b) is arranged on the first bottom plate (221 a), the first bottom plate (221 a) is connected with the first linear motion mechanism (21), the first flanges (221 b) are two and mutually parallel, and are mutually parallel to the second flange (221 d) to form a shape, a plurality of first spring plungers are respectively arranged on opposite sides of the two first flanges (221 b), a plurality of positioning bosses are arranged on the second flange (221 d), a plurality of first positioning grooves (222 f) and a plurality of first positioning holes (222 g) are formed in the side wall of the first bottom frame (222 b), the first positioning grooves (222 f) are matched with the positioning bosses to limit, the first positioning holes (222 g) are matched with the first spring plungers to limit, and the first elastic reset mechanism (221 c) at least comprises a first spring plunger and a second spring plunger which is in a released state from the first spring plunger.
6. The automated biological sample dispensing apparatus of claim 5, wherein: the clamping plate mechanism comprises a plurality of clamping plates, each clamping plate is sequentially arranged along the Z axis and is connected with the sixth connecting seat (223 p), a plurality of second through holes are formed in the clamping plates, the area where the second through holes of each clamping plate are located is sequentially arranged along the moving direction of the clamping plate mechanism, and the sum of the numbers of the second through holes is equal to the number of the first through holes of the first cover plate (222 a).
7. The automated biological sample dispensing apparatus of claim 1, wherein: the cap screwing mechanism (4) further comprises a ranging sensor (44) and a second scanner (45), the cap screwing assembly (43) comprises a square frame (431), a second partition plate (432), a third partition plate (433) and a cap screwing driving mechanism (434), the second partition plate (432) and the third partition plate (433) are horizontally arranged in the square frame (431), the ranging sensor (44) is connected with the square frame (431) and used for detecting whether a cap body of the sampling tube (6) is unscrewed, the second scanner (45) is connected with a fourth linear motion mechanism (42) and used for scanning a bar code on the sampling tube (6), the cap screwing driving mechanism (434) comprises a second motor (434 a), a first coupler (434 b), a key shaft (434 c), a key shaft sliding sleeve (434 d), a third spring (434 e), a key shaft (434 f), a first pin shaft (434 g) and clamping jaws, the second motor (a) is connected with the key shaft (434 b) through the first coupler (434 b) and is connected with the key shaft (434 c) through the second coupler (434 c) and is fixedly connected with the key shaft (434 d) through the second coupler (434 d) and is connected with the key shaft (434 d) through the other end (434 d), the key shaft sliding sleeve (434 d) is in sliding connection with the third partition plate (433), the third spring (434 e) is sleeved outside the key shaft sliding sleeve (434 d) and limited between the first pin shaft (434 g) and the third partition plate (433), when the cover is screwed, the cover screwing assembly (43) aligns the sampling tube (6), and drives the clamping jaw to screw the cover body of the sampling tube (6) through the second motor (434 a).
8. The automated biological sample dispensing apparatus of claim 7, wherein: the clamping jaw comprises a rotating cover head (434 h), a plurality of rotating cover clamping pages (434 i), a plurality of fourth springs (434 j), a plurality of second pin shafts (434 k) and a plurality of first ejector pins (434 l), wherein the rotating cover head (434 h) is a two-section stepped shaft, a small end shaft section is sleeved in the key shaft sliding sleeve (434 d) and is vertically penetrated and connected through the first pin shafts (434 g), a plurality of first through grooves are formed in the annular direction of a large end shaft section, through holes perpendicular to an axis are formed in two sides of each first through groove, the rotating cover clamping pages (434 i) are in one-to-one correspondence with the first through grooves, the second pin shafts (434 k) penetrating through the through holes are hinged, and two ends of the fourth springs (434 j) respectively correspondingly abut against the space between the rotating cover clamping pages (434 i) and the first through grooves and push the rotating cover clamping pages (434 i) to rotate along the second pin shafts (434 k) to clamp the tip end of the sampling tube (6), and the rotating cover clamping pages (434 i) are arranged at the tip end sides of the rotating cover clamping heads (434 h) are arranged on the outer sides of the rotating cover body.
9. The automated biological sample dispensing apparatus of claim 1, wherein: the secondary liquid taking assembly (52) comprises a sixth linear motion mechanism (521), a seventh linear motion mechanism (522), a straw dismounting mechanism (523) and a limiting plate (524), wherein:
The sixth linear motion mechanism (521) is connected with the fifth linear motion mechanism (51) and drives the seventh linear motion mechanism (522) to move along the Z axis;
the seventh linear motion mechanism (522) is connected to the sixth linear motion mechanism 521 and drives the suction pipe attaching/detaching mechanism (523) to move in the Z-axis direction;
the suction pipe dismounting mechanism (523) comprises a ninth connecting seat (523 a), a plunger pump (523 b), a sleeve (523 d), a first guide seat (523 e), a second guide seat (523 f), a top plate (523 g), a dismounting mechanism (523 h), a fifth spring (523 i) and a pressure sensor (523 c), wherein the ninth connecting seat (523 a) is connected with a seventh linear motion mechanism (522), the plunger pump (523 b), the first guide seat (523 e) and the second guide seat (523 f) are connected with the ninth connecting seat (523 a), the dismounting mechanism (523 h) comprises a dismounting seat (523 j) and at least one fourth guide rod (523 k) which are connected with each other, a third avoiding hole (523 l) is formed in the dismounting seat (523 j), one end of the sleeve (523 d) is connected with the plunger pump (523 b) in parallel with the fourth guide rod (523 k), the other end of the sleeve (523 d) is used for detecting the pressure sensor (523 j) and the fourth guide seat (523 k) which is connected with the fourth guide seat (523 f) in a limited manner, the fifth spring (523 i) is sleeved on the fourth guide rod (523 k), two ends of the fifth spring respectively lean against the disassembly seat (523 j) and the second guide seat (523 f), and the top plate (523 g) is fixedly connected to one end of the fourth guide rod (523 k) and is positioned at one side, deviating from the disassembly seat (523 j), of the second guide seat (523 f);
The limiting plate (524) is connected with the fixing part of the sixth linear motion mechanism (521), and abuts against the disassembling seat (523 j) to limit when the straw disassembling mechanism (523) moves upwards, so that the straw is quickly disassembled.
10. The automatic biological sample dispensing device according to any one of claims 1 to 9, wherein: each linear motion mechanism is a screw nut transmission mechanism or a synchronous belt transmission mechanism.
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| WO2020151368A1 (en) * | 2019-01-23 | 2020-07-30 | 安图实验仪器(郑州)有限公司 | Fully automatic microorganism identification and drug sensitivity analysis system |
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