CN111647503B - Magnetic bead method nucleic acid detection kit - Google Patents
Magnetic bead method nucleic acid detection kit Download PDFInfo
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- CN111647503B CN111647503B CN202010654730.9A CN202010654730A CN111647503B CN 111647503 B CN111647503 B CN 111647503B CN 202010654730 A CN202010654730 A CN 202010654730A CN 111647503 B CN111647503 B CN 111647503B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/42—Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2547/00—Reactions characterised by the features used to prevent contamination
- C12Q2547/10—Reactions characterised by the features used to prevent contamination the purpose being preventing contamination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/149—Particles, e.g. beads
Abstract
The utility model provides a magnetic bead method nucleic acid detect kit, including reaction mechanism, extract the mechanism, detection mechanism, this device simple structure, the detachable design of supporting component and spacing subassembly can select the use quantity according to measuring quantity, avoid causing the waste, and the supporting component takes effect to extracting the mechanism in the testing process, avoid the magnetic bead to think the contact because of magnetic action, cause cross infection, spacing subassembly is when the magnetic bead gets into the detection box, the control magnetic bead is changeed in droing, avoid being taken away the pollution reagent because of magnetic action magnetic bead.
Description
Technical Field
The invention belongs to the field of nucleic acid detection, and particularly relates to a nucleic acid detection kit by a magnetic bead method.
Background
The magnetic bead method nucleic acid detection kit plays an important role in the nucleic acid detection process, is one of indispensable tools in nucleic acid extraction and detection processes, and the traditional nucleic acid detection kit has a simple structure, does not have an effective isolation protection device for magnetic beads after nucleic acid extraction, easily makes the magnetic beads collide and contact under the action of magnetic force to cause cross infection, and when the detection kit is put into after extraction, the magnetic beads are not easy to fall off under the action of magnetic bars.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention provides the magnetic bead method nucleic acid detection kit which avoids magnetic bead contact in the nucleic acid detection process and is convenient for magnetic bead separation.
In order to achieve the purpose, the invention adopts the following technical scheme: the utility model provides a magnetic bead method nucleic acid detect kit, includes reaction mechanism and draws mechanism and detection mechanism, draw the mechanism including draw the board, can dismantle six magnetic stripe spaces that set up on drawing the board, fix and set up five stoppers of drawing the board lower extreme, can dismantle the spacing spout that sets up on the stopper, fix the first compression spring that sets up at spacing spout lower extreme, the fixed supporting component who sets up at first compression spring lower extreme, six magnetic stripe spaces evenly set up on drawing the board, five stoppers evenly set up between per two magnetic stripe spaces.
The support assembly comprises a support box fixedly arranged at the lower end of the first compression spring, two support rods rotatably arranged on the support box, a torsional spring fixedly arranged in the support rods, a sliding groove fixedly arranged at the lower end of the support box, a sliding rod capable of sliding back and forth arranged on the sliding groove, a guide plate fixedly arranged at the upper end of the sliding rod, a cotton rope fixedly arranged between the guide plate and the support rods, and a support plate fixedly arranged at the lower end of the sliding rod, wherein the two support rods are bilaterally symmetrical relative to the support box, one end of the torsional spring is fixedly connected with the support rods, the other end of the torsional spring is fixedly connected with the support box, the sliding rod penetrates through the sliding groove, one end of the sliding rod is arranged in the support box.
The supporting component further comprises two second compression springs fixedly arranged between the upper end face of the supporting plate and the inner wall of the upper side of the sliding groove, and the two second compression springs are bilaterally symmetrical relative to the sliding rod.
The reaction mechanism comprises a shell and a plurality of reaction boxes which are detachably arranged in the shell; the reaction box comprises a reaction cavity, a reaction space arranged in the reaction cavity, and two high baffles and two low baffles which are fixedly arranged at the upper end of the reaction cavity, wherein the two high baffles and the two low baffles are fixedly connected in sequence.
The detection mechanism comprises a frame, a detection cavity arranged in the frame in a detachable mode, a detection space arranged in the detection cavity, and a limiting assembly arranged on the detection cavity in a detachable mode.
The limiting assembly comprises a U-shaped groove, a connecting rod which is rotatably arranged on the U-shaped groove, a third compression spring which is fixedly arranged on the lower end face of the connecting rod, an arc plate which is fixedly arranged at the other end of the third compression spring, a spherical block which is rotatably arranged in the arc plate, and a limiting block which is fixedly arranged on the spherical block, wherein the arc plate surrounds two thirds of the spherical block.
In addition, the invention also provides a using method of the nucleic acid detection kit by the magnetic bead method, which comprises the following steps:
the reaction box that will correspond quantity according to detection quantity before nucleic acid draws places in the shell, avoids the waste that whole box used to cause the kit, and high baffle is along the digital direction during the placement, and low baffle is placed along the alphabetical direction, and the follow-up magnetic bead of being convenient for draws, puts into the reaction space of reaction cavity with detector blood sample in, puts into reaction space with the magnetic bead again, waits for the magnetic bead to adsorb nucleic acid.
After adsorption, according to the nucleic acid detection quantity, selecting a corresponding quantity of magnetic stripe spaces to be placed on an extraction plate, simultaneously placing a support component at the lower end of the extraction plate, sliding a limit chute into the limit block along the track of the limit block during placement, fixing the support component at the lower end of the extraction plate, enabling the support component to be located between the two magnetic stripe spaces, in an initial state, enabling the two support rods to be in a horizontal state under the action of a torsional spring, forming rigid support for the magnetic stripe spaces at the two sides of the support component, enabling an extraction mechanism to be more stable, placing a magnetic rod into the magnetic stripe spaces during extraction of magnetic beads, placing the extraction mechanism in a reaction space of a reaction cavity, enabling an opening of the chute to be placed above a low baffle, enabling the lower end face of the support plate to be in contact with the upper end face of the low baffle during descending of the extraction mechanism, enabling the support plate to move upwards in the chute through, the slide bar shifts up the in-process and drives the deflector and shifts up, the pulling force that the deflector produced through the cotton rope is acted on the bracing piece, make the bracing piece take place the angle transformation downwards, the torsional spring holds power, two bracing pieces change soft support into to the rigid support in magnetic stripe space, make whole extraction mechanism move more smoothly in reaction space, avoid extracting the mechanism and move down the in-process and take place to scratch with the frame inner wall, whole supporting component takes place to move up between two magnetic stripe spaces simultaneously, compress first compression spring, when the magnetic bar contacts with the magnetic bead, the magnetic bead is attracted by the magnetic bar, shift up and extract the mechanism, take out the magnetic bead from reaction space.
The extraction mechanism moves up the in-process, first compression spring resets and drives the supporting component and descend and restore to the initial position, two second compression springs reset simultaneously, it moves down to promote the backup pad, the backup pad moves down the in-process and drives the deflector through the slide bar and move down, make the cotton rope reset, the torsional spring that holds power simultaneously resets, make two bracing pieces rotate back the initial position, form rigid support again to the magnetic stripe space of the supporting component left and right sides, avoid two magnetic stripe spaces inter attraction under the magnetic bar magnetic force effect, make the magnetic bead contact, cause cross infection, the supporting component's that descends spout extends to magnetic stripe space lower extreme simultaneously, form the baffler to absorbent magnetic bead under the magnetic stripe space, make the magnetic bead be in independent space, the condition of having avoided the magnetic bead contact once more takes place.
After extraction, magnetic beads are placed in front of the detection mechanism, a corresponding number of detection cavities are placed in the frame according to different detection quantities, waste of the detection box is avoided, meanwhile, U-shaped grooves of the two limiting assemblies are placed at the upper ends of the detection cavities, limiting blocks of the two limiting assemblies are respectively contacted with the inner walls of the left side and the right side of the detection space, in the process of placing the magnetic beads, the magnetic beads are firstly contacted with the upper end face of a connecting rod, the connecting rod is compressed on the U-shaped grooves to rotate downwards along with the downward movement of the magnetic beads, a third compression spring is compressed by the rotation of the connecting rod to generate elastic potential energy, the arc-shaped plate is acted by the third compression spring to generate angle transformation on the spherical block to apply pressure to the limiting blocks, friction between the limiting blocks and the detection space is increased, the rotating angle of the connecting rod is larger and larger along with the downward movement depth of, move up the magnetic stripe space, spacing angle because of the connecting rod forms produces the hindrance to the magnetic bead, make the magnetic stripe space break away from outside the detection space more easily, the magnetic bead drops in the detection space, avoid the magnetic stripe space to move up the in-process and take the magnetic bead out, cause unnecessary trouble, the magnetic stripe space removes the back, third compression spring resets, promote the connecting rod and take place ascending angle in "U" type groove and turn to, make two connecting rods keep level, avoid magnetic bead and external too much contact, cause the pollution.
In summary, the invention has the following advantages: this device simple structure, the detachable design of supporting component and spacing subassembly can select the use quantity according to the detection quantity, avoids causing the waste, and supporting component takes effect to extracting the mechanism in the testing process, avoids the magnetic bead to think the contact because of magnetic force action, causes cross infection, and spacing subassembly is when the magnetic bead gets into the detection box, and the control magnetic bead is changeed in droing, avoids being taken away out the contaminated reagent because of magnetic force action magnetic bead.
Drawings
FIG. 1 is a diagram of a reaction mechanism embodiment of the present invention;
FIG. 2 is a schematic view of the reaction cassette of FIG. 1;
FIG. 3 is a diagram of an embodiment of an extraction mechanism of the present invention;
FIG. 4 is an enlarged schematic view taken at A in FIG. 3;
FIG. 5 is an enlarged view of the point B in FIG. 3;
FIG. 6 is a view of an embodiment of the detecting mechanism of the present invention;
FIG. 7 is a schematic view showing the construction of the cartridge of FIG. 6;
FIG. 8 is an enlarged view of the point C in FIG. 7;
FIG. 9 is an enlarged view of FIG. 7 at D;
Detailed Description
As shown in FIGS. 1-9, a nucleic acid detecting kit by magnetic bead method comprises a reaction mechanism, an extraction mechanism and a detection mechanism, wherein the extraction mechanism comprises an extraction plate 14, six magnetic stripe spaces 15 detachably arranged on the extraction plate 14, five limit blocks 18 fixedly arranged at the lower end of the extraction plate 14, limit chutes 19 detachably arranged on the limit blocks 18, first compression springs 20 fixedly arranged at the lower ends of the limit chutes 19, and support members fixedly arranged at the lower ends of the first compression springs 20, wherein the six magnetic stripe spaces 15 are uniformly arranged on the extraction plate 14, the five limit blocks 18 are uniformly arranged between every two magnetic stripe spaces 15, according to the nucleic acid detection quantity, a corresponding quantity of magnetic stripe spaces 15 are selected to be arranged on the extraction plate 14, the support members are arranged at the lower end of the extraction plate 14, and the limit chutes 19 slide into the limit blocks 18 along the tracks of the limit blocks 18 during arrangement, the support member is fixed to the lower end of the extracting plate 14 such that the support member is located between the two magnetic stripe spaces 15.
The support component comprises a support box 22 fixedly arranged at the lower end of a first compression spring 20, two support rods 16, a torsion spring 21, a sliding groove 17, a sliding rod 25, a guide plate 24, a cord 23 and a support plate 26, wherein the two support rods 16 are rotatably arranged on the support box 22, the torsion spring 21 is fixedly arranged in the support rods 16, the sliding groove 17 is fixedly arranged at the lower end of the support box 22, the sliding rod 25 is arranged on the sliding groove 17 in a reciprocating sliding manner, the guide plate 24 is fixedly arranged at the upper end of the sliding rod 25, the cord 23 is fixedly arranged between the guide plate 24 and the support rods 16, the support plate 26 is fixedly arranged at the lower end of the sliding rod 25, the two support rods 16 are bilaterally symmetrical relative to the support box 22, one end of the torsion spring 21 is fixedly connected with the support rod 16, the other end of the torsion spring is fixedly connected with the support box 22, the sliding rod 25 penetrates through, the supporting plate 26 can slide back and forth in the sliding groove 17, in an initial state, the two supporting rods 16 are in a horizontal state under the action of the torsion spring 21, hard supports are formed on the magnetic strip spaces 15 on the two sides of the supporting component, so that the extraction mechanism is more stable, when magnetic beads are extracted, the magnetic bars are placed in the magnetic strip spaces 15, the extraction mechanism is placed in the reaction space 38 of the reaction cavity 11, the opening of the sliding groove 17 is placed above the low baffle 13, in the descending process of the extraction mechanism, the lower end face of the supporting plate 26 is in contact with the upper end face of the low baffle 13, along with the increase of the descending depth, the supporting plate 26 moves upwards in the sliding groove 17 through the sliding rod 25, the second compression spring 27 is compressed to generate elastic potential energy, in the upwards moving process of the sliding rod 25, the guide plate 24 is driven to move upwards, the guide plate 24 acts on the supporting rods 16 through the tension force generated by, the hard support of the two support rods 16 to the magnetic stripe space 15 is changed into soft support, so that the whole extraction mechanism moves more smoothly in the reaction space 38, the phenomenon that the extraction mechanism scratches the inner wall of the frame 28 in the moving-down process is avoided, meanwhile, the whole support assembly moves upwards between the two magnetic stripe spaces 15, the first compression spring 20 is compressed, when the magnetic rod is contacted with the magnetic bead, the magnetic bead is attracted by the magnetic rod, the magnetic bead is moved upwards to the extraction mechanism to be taken out of the reaction space 38, in the moving-up process of the extraction mechanism, the first compression spring 20 resets to drive the support assembly to descend to the initial position, meanwhile, the two second compression springs 27 reset to push the support plate 26 to move downwards, in the moving-down process of the support plate 26, the guide plate 24 is driven by the slide rod 25 to move downwards to reset the thread rope 23, simultaneously, the force-accumulating torsion spring 21 resets to enable the two support rods 16 to rotate back to the initial position, and form the magnetic, avoid two magnetic stripe spaces 15 inter attraction under bar magnet magnetic force effect, make the magnetic bead contact, cause cross infection, the supporting component's that descends simultaneously spout 17 extends to the 15 lower extremes in magnetic stripe space, forms the baffler to the adsorbed magnetic bead under the magnetic stripe space 15, makes the magnetic bead be in independent space, has avoided the condition of magnetic bead contact to take place once more.
The support assembly further comprises two second compression springs 27 fixedly arranged between the upper end surface of the support plate 26 and the upper inner wall of the slide groove 17, and the two second compression springs 27 are bilaterally symmetrical about the slide rod 25.
The reaction mechanism comprises a shell 10 and a plurality of reaction cartridges which are detachably arranged in the shell 10; the reaction box comprises a reaction cavity 11, a reaction space 38, two high baffles 12 and two low baffles 13, wherein the reaction space 38 is arranged in the reaction cavity 11, the two high baffles 12 and the two low baffles 13 are fixedly arranged at the upper end of the reaction cavity 11, the two high baffles 12 and the two low baffles 13 are fixedly connected in sequence, the reaction boxes with corresponding quantities are placed in the shell 10 according to the detection quantity before nucleic acid extraction, the waste of the kit caused by the use of the whole box is avoided, the high baffles 12 are placed along the digital direction when the whole box is placed, the low baffles 13 are placed along the letter direction, the subsequent magnetic beads are conveniently extracted, a blood sample of a detector is placed in the reaction space 38 of the reaction cavity 11, then the magnetic beads are placed in the reaction space 38, and the magnetic beads are waited for adsorbing the nucleic acids.
Detection mechanism includes frame 28, detection cavity 29, detection space 37, spacing subassembly, detection cavity 29 can dismantle the setting in frame 28, detection space 37 sets up in detection cavity 29, spacing subassembly can dismantle the setting on detection cavity 29, draws the back, places the magnetic bead before detection mechanism, can place the detection cavity 29 of corresponding quantity in frame 28 according to the detection quantity of difference equally, avoids causing the waste of detection box.
The limiting components comprise a U-shaped groove 30, a connecting rod 33, a third compression spring 32, an arc-shaped plate 34, a spherical block 35 and a limiting block 36, the connecting rod 33 is rotatably arranged on the U-shaped groove 30, the third compression spring 32 is fixedly arranged on the lower end face of the connecting rod 33, the arc-shaped plate 34 is fixedly arranged at the other end of the third compression spring 32, the spherical block 35 is rotatably arranged in the arc-shaped plate 34, the limiting block 36 is fixedly arranged on the spherical block 35, the arc-shaped plate 34 surrounds two thirds of the spherical block 35, the U-shaped grooves 30 of the two limiting components are arranged at the upper end of the detection cavity 29, the limiting blocks 36 of the two limiting components are respectively contacted with the inner walls of the left side and the right side of the detection space 37, in the process of placing the magnetic beads, the magnetic beads are firstly contacted with the upper end face of the connecting rod 33, and along with the downward movement of the magnetic beads, the connecting rod 33 is, the third compression spring 32 is compressed by the rotation of the connecting rod 33 to generate elastic potential energy, the third compression spring 32 acts on the arc-shaped plate 34 to generate angle transformation on the spherical block 35 to apply pressure on the limiting block 36, the friction between the limiting block 36 and the detection space 37 is increased, the rotating angle of the connecting rod 33 is increased along with the downward movement of the magnetic beads, after the magnetic beads completely enter the detection space 37, the magnetic bead space 15 is moved upwards, the magnetic bead space 15 is more easily separated from the detection space 37 due to the obstruction of the limiting angle formed by the connecting rod 33, the magnetic bead falls into the detection space 37, the magnetic bead is prevented from being brought out in the upward movement process of the magnetic bead space 15, unnecessary troubles are avoided, after the magnetic bead space 15 is removed, the third compression spring 32 is reset to push the connecting rod 33 to generate upward angle rotation on the U-shaped groove 30, the two connecting rods 33 are kept level, and the magnetic beads are prevented from being excessively contacted, causing pollution.
The working principle is as follows: place the reaction box that will correspond quantity according to detection quantity before nucleic acid draws in shell 10, avoid the full box to use the waste that causes the kit, high baffle 12 is along the digital direction during the placement, and low baffle 13 is placed along the alphabetical direction, and follow-up magnetic bead of being convenient for draws, puts into reaction space 38 of reaction cavity 11 with the person's of detection blood sample, puts into reaction space 38 with the magnetic bead again, waits for the magnetic bead to adsorb nucleic acid.
After adsorption, according to the number of nucleic acid detection, selecting a corresponding number of magnetic stripe spaces 15 to be placed on the extraction plate 14, simultaneously placing a support component at the lower end of the extraction plate 14, sliding a limit sliding groove 19 into a limit block 18 along the track of the limit block 18 during placement, fixing the support component at the lower end of the extraction plate 14 to enable the support component to be positioned between the two magnetic stripe spaces 15, in an initial state, enabling the two support rods 16 to be in a horizontal state under the action of a torsion spring 21 to form rigid support for the magnetic stripe spaces 15 at the two sides of the support component to enable the extraction mechanism to be more stable, placing the magnetic rods into the magnetic stripe spaces 15 during extraction of magnetic beads, placing the extraction mechanism in a reaction space 38 of the reaction cavity 11 to enable the opening of the sliding groove 17 to be placed above the low baffle 13, in the descending process of the extraction mechanism, enabling the lower end face of the support plate 26 to be in contact with the upper end face of the low baffle 13, the second compression spring 27 is compressed to generate elastic potential energy, the slide rod 25 drives the guide plate 24 to move upwards in the moving-up process, the guide plate 24 acts on the support rod 16 through the pulling force generated by the cotton rope 23, the support rod 16 is enabled to change downwards in angle, the torsion spring 21 stores force, the two support rods 16 change the hard support of the magnetic stripe space 15 into the soft support, the whole extraction mechanism can move more smoothly in the reaction space 38, the scraping between the extraction mechanism and the inner wall of the frame 28 in the moving-down process is avoided, meanwhile, the whole support assembly moves upwards between the two magnetic stripe spaces 15, the first compression spring 20 is compressed, when the magnetic bar is in contact with the magnetic bead, the magnetic bead is attracted by the magnetic bar, the extraction mechanism moves upwards, and the magnetic bead is taken out from the reaction space 38.
In the upward moving process of the extraction mechanism, the first compression spring 20 resets to drive the supporting component to descend and restore to the initial position, the two second compression springs 27 reset simultaneously, the supporting plate 26 is pushed to move downwards, the guide plate 24 is driven to move downwards through the sliding rod 25 in the downward moving process of the supporting plate 26, the cotton rope 23 resets, the torsion spring 21 which stores power simultaneously resets, the two supporting rods 16 rotate back to the initial position, the magnetic strip spaces 15 on the left side and the right side of the supporting component form rigid supports again, the two magnetic strip spaces 15 are prevented from being mutually attracted under the magnetic force action of the magnetic rods, magnetic beads are enabled to be contacted, cross infection is caused, the sliding grooves 17 of the simultaneously-descending supporting component extend to the lower end of the magnetic strip spaces 15, the magnetic beads adsorbed under the magnetic strip spaces 15 form a blocking plate, the magnetic beads are enabled to be.
After extraction, magnetic beads are placed in front of the detection mechanism, a corresponding number of detection cavities 29 are placed in the frame 28 according to different detection quantities, waste of the detection box is avoided, meanwhile, the U-shaped grooves 30 of the two limiting assemblies are placed at the upper ends of the detection cavities 29, limiting blocks 36 of the two limiting assemblies are respectively contacted with the inner walls of the left side and the right side of a detection space 37, in the process of placing the magnetic beads, the magnetic beads are firstly contacted with the upper end face of a connecting rod 33, the connecting rod 33 is compressed on the U-shaped groove 30 to rotate downwards along with downward movement of the magnetic beads, the third compression spring 32 is compressed by the rotation of the connecting rod 33 to generate elastic potential energy, the arc-shaped plate 34 acts on a ball 35 to generate angular transformation to apply pressure to the limiting blocks 36 so as to increase friction between the limiting blocks 36 and the detection space 37, and the rotating angle of the connecting rod 33 is larger and larger along with the deeper downward movement, after the magnetic bead completely got into detection space 37, go up magnetic stripe space 15, spacing angle because of connecting rod 33 forms produced the hindrance to the magnetic bead, make magnetic stripe space 15 break away from outside detection space 37 more easily, the magnetic bead drops in detection space 37, avoid magnetic stripe space 15 to move up the in-process and take the magnetic bead out of, cause unnecessary trouble, magnetic stripe space 15 removes the back, third compression spring 32 resets, promote connecting rod 33 and take place ascending angle and transfer on "U" type groove 30, make two connecting rods 33 keep level, avoid magnetic bead and external too much contact, cause the pollution.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are given by way of illustration of the principles of the present invention, and that various changes and modifications may be made without departing from the spirit and scope of the invention as defined by the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (4)
1. The utility model provides a magnetic bead method nucleic acid detect reagent box, includes reaction mechanism and draws mechanism and detection mechanism, its characterized in that: the extraction mechanism comprises an extraction plate (14), six magnetic stripe spaces (15) detachably arranged on the extraction plate (14), five first limiting blocks (18) fixedly arranged at the lower end of the extraction plate (14), a limiting sliding groove (19) detachably arranged on the first limiting block (18), a first compression spring (20) fixedly arranged at the lower end of the limiting sliding groove (19) and a support assembly fixedly arranged at the lower end of the first compression spring (20), wherein the six magnetic stripe spaces (15) are uniformly arranged on the extraction plate (14), and the five first limiting blocks (18) are uniformly arranged between every two magnetic stripe spaces (15); the reaction mechanism comprises a shell (10) and a plurality of reaction boxes which are detachably arranged in the shell (10); the reaction box comprises a reaction cavity (11), a reaction space (38) arranged in the reaction cavity (11), and two high baffles (12) and two low baffles (13) which are fixedly arranged at the upper end of the reaction cavity (11), wherein the two high baffles (12) and the two low baffles (13) are sequentially and fixedly connected; the detection mechanism comprises a frame (28), a detection cavity (29) detachably arranged in the frame (28), a detection space (37) arranged in the detection cavity (29) and a limiting assembly detachably arranged on the detection cavity (29).
2. The kit for detecting nucleic acid by using a magnetic bead method according to claim 1, wherein: the support assembly comprises a support box (22) fixedly arranged at the lower end of a first compression spring (20), two support rods (16) rotatably arranged on the support box (22), a torsion spring (21) fixedly arranged in the support rods (16), a sliding chute (17) fixedly arranged at the lower end of the support box (22), a sliding rod (25) arranged on the sliding chute (17) in a reciprocating sliding manner, a guide plate (24) fixedly arranged at the upper end of the sliding rod (25), a thread rope (23) fixedly arranged between the guide plate (24) and the support rods (16) and a support plate (26) fixedly arranged at the lower end of the sliding rod (25), the two support rods (16) are bilaterally symmetrical relative to the support box (22), one end of the torsion spring (21) is fixedly connected with the support rods (16), the other end of the torsion spring is fixedly connected with the support box (22), the sliding rod (25) penetrates through the sliding chute (17), and one end of the sliding rod is arranged in, the other end is arranged in the sliding groove (17), and the supporting plate (26) can slide in the sliding groove (17) in a reciprocating way.
3. The kit for detecting nucleic acid by using a magnetic bead method according to claim 2, wherein: the supporting component also comprises two second compression springs (27) fixedly arranged between the upper end surface of the supporting plate (26) and the inner wall of the upper side of the sliding groove (17), and the two second compression springs (27) are bilaterally symmetrical relative to the sliding rod (25).
4. The kit for detecting nucleic acid by using a magnetic bead method according to claim 1, wherein: the limiting assembly comprises a U-shaped groove (30), a connecting rod (33) rotatably arranged on the U-shaped groove (30), a third compression spring (32) fixedly arranged on the lower end face of the connecting rod (33), an arc plate (34) fixedly arranged at the other end of the third compression spring (32), a spherical block (35) rotatably arranged in the arc plate (34) and a second limiting block (36) fixedly arranged on the spherical block (35), wherein the arc plate (34) surrounds two-thirds of the spherical block (35).
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010654730.9A CN111647503B (en) | 2020-07-09 | 2020-07-09 | Magnetic bead method nucleic acid detection kit |
| GB2105151.1A GB2596895A (en) | 2020-07-09 | 2021-04-12 | A nucleic acid detection kit by magnetic beads methods |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010654730.9A CN111647503B (en) | 2020-07-09 | 2020-07-09 | Magnetic bead method nucleic acid detection kit |
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| Publication Number | Publication Date |
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| CN111647503A CN111647503A (en) | 2020-09-11 |
| CN111647503B true CN111647503B (en) | 2021-05-25 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010654730.9A Active CN111647503B (en) | 2020-07-09 | 2020-07-09 | Magnetic bead method nucleic acid detection kit |
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| CN (1) | CN111647503B (en) |
| GB (1) | GB2596895A (en) |
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Also Published As
| Publication number | Publication date |
|---|---|
| GB202105151D0 (en) | 2021-05-26 |
| GB2596895A (en) | 2022-01-12 |
| CN111647503A (en) | 2020-09-11 |
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