CN108456636A - A kind of infull detection kit of AIDS immunity function restructuring - Google Patents
A kind of infull detection kit of AIDS immunity function restructuring Download PDFInfo
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- CN108456636A CN108456636A CN201810294174.1A CN201810294174A CN108456636A CN 108456636 A CN108456636 A CN 108456636A CN 201810294174 A CN201810294174 A CN 201810294174A CN 108456636 A CN108456636 A CN 108456636A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/702—Specific hybridization probes for retroviruses
- C12Q1/703—Viruses associated with AIDS
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- Genetics & Genomics (AREA)
- AIDS & HIV (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a kind of infull detection kits of AIDS immunity function restructuring, including box cover and box body, box cover is connected to by articulated manner on box body, multiple lattices are separated by partition board in box cover, respectively positive reference substance lattice, detect instrument lattice, negative controls lattice, detection reagent lattice, clean tube lattice and test strip lattice, multiple cover boards are corresponded with lattice, it is offered in the top inner wall of each lattice and the matched erection slot of cover board, it is provided with rack in detection reagent lattice, test tube placed cavity is offered in rack, the bottom of test tube placed cavity is fixedly installed placing groove, clamping device for being gripped to detection reagent test tube is installed in the upper inside wall of test tube placed cavity.The configuration of the present invention is simple, it is rationally distributed, storage can be separated for the AIDS immunity function restructuring various articles that full inspection is not surveyed, and pick and place conveniently, effectively increase the working efficiency of doctor.
Description
Technical field
The present invention relates to a kind of AIDS detection kit, specifically a kind of AIDS immunity function restructuring not test by full inspection
Agent box.
Background technology
Human immunodeficiency virus is to lead to a kind of pathogenic microorganism of chronic lethal disease AIDS.Due to AIDS
With infectiousness and high lethal, therefore have become one of the principal disease of emphasis prevention and control in world wide.It ends from first case
Sick case has been grown since 1981 report AIDS case for the first time, and up to the present, AIDS has resulted in more than 2,005 million peoples
It is dead.China is since 1985 find AIDS case for the first time, and the prevalence of AIDS is in tendency of quickly climbing.In recent years, in
The infection of state's AIDS also increases comparatively fast with number of the infected.China's AIDS present situation is stationed in China the data that mechanism shows according to the United Nations,
About 840,000 people of China's AIDS virus the infected at present, in addition 240,000 people to have passed away, sum should be in 1,000,000 people or so.Root
Predicted according to the World Health Organization, although although at present China's AIDS virus carrier account for total population ratio it is very low, infection
Total number of persons occupies the 2nd in Asia, and the 14th is occupied in the whole world.It is that world population radix is maximum in view of China, population is the most intensive
Country, once the extensive prevalence of AIDS will become a public health disaster.The currently used diagnostic means of diagnosis of HIV
Including viral diagnosis and serodiagnosis, the viral diagnosis technology common method of HIV infection is co-cultivation method, that is, is used normal
Human peripheral blood detaches mononuclearcell, after adding PHA to stimulate and cultivate, patient's mononuclearcell is added and carries out examining for AIDS
It is disconnected.
AIDS infection risk covers general population, therefore AIDS detection and prevention are a kind of important topic classes.Currently,
Doctor generally requires to use many detection articles for use when not being detected AIDS immunity function restructuring entirely.Common reagent
Box cannot store various required articles, inconvenient for use, and side does not facilitate the placement and fixation of blood samples test tube, gives the inspection of doctor
Surveying operation makes troubles.
Invention content
The purpose of the present invention is to provide a kind of infull detection kits of AIDS immunity function restructuring, to solve the above-mentioned back of the body
The problem of being proposed in scape technology.
To achieve the above object, the present invention provides the following technical solutions：
A kind of infull detection kit of AIDS immunity function restructuring, including box cover and box body, the box cover are one whole
Body lid, box cover are connected to by articulated manner on box body, and multiple lattices are separated by partition board in the box cover, respectively positive
Reference substance lattice, detection instrument lattice, negative controls lattice, detection reagent lattice, clean tube lattice and test strip
Lattice, six lattices are arranged in front two rows, each three lattices of front two rows, and multiple cover boards are corresponded with lattice, each
It is offered in the top inner wall of lattice and the matched erection slot of cover board；It is provided with rack in the detection reagent lattice,
Offer test tube placed cavity in rack, the bottom of test tube placed cavity is fixedly installed placing groove, the test tube placed cavity it is upper
Clamping device for being gripped to detection reagent test tube is installed, the clamping device includes supporting sleeve on portion's inner wall
Cylinder, arc fixture block, spring, sliding block and compression bar, wherein stop sleeve are fixedly mounted in the upper inside wall of test tube placed cavity, sliding block
Level sliding be set to stop sleeve in, spring is additionally provided in stop sleeve, the side of sliding block is connected with spring, sliding block it is another
Side is connected with compression bar, and arc fixture block is fixedly mounted on the other end of compression bar.
As a further solution of the present invention：It is provided with handle on the end outer wall of the box cover.
As a further solution of the present invention：It is both provided with label box at the top of the cover board.
As a further solution of the present invention：The label box is made of transparent plastic.
As a further solution of the present invention：The concave side surface of the arc fixture block is provided with buffering spacer.
As a further solution of the present invention：The buffering spacer is made of rubber material.
As a further solution of the present invention：The box cover is fixed on by electronic password lock on box body.
As a further solution of the present invention：The detection reagent further includes paramagnetic particle method nucleic acid extracting reagent and HIV nucleic acid
Amplifing reagent, wherein paramagnetic particle method nucleic acid extraction kit include cracking in conjunction with liquid, rinsing liquid A, rinsing liquid B, rinsing liquid C, elution
Liquid, magnetic bead liquid；HIV nucleic acid amplification kits include RT-PCR reaction solutions, enzyme mixation, HIV- internal standards, HIV qualitative reference product 1-
4, negative quality-control product, critical positive quality control product, strong positive quality-control product.
As a further solution of the present invention：Cracking combines liquid group as containing ten in the paramagnetic particle method nucleic acid extracting reagent
Sodium dialkyl sulfate 0.8-2.0%, guanidinium isothiocyanate 3.5-5.5mol/L, 1-10mM/LEDTA (PH7.5)；The paramagnetic particle method core
Rinsing liquid A groups, which become, in sour extracts kit contains TritonX-1000.5-2.0ml/100ml, lithium chloride 0.5-1mol/L；Institute
Stating rinsing liquid B groups in paramagnetic particle method nucleic acid extraction kit becomes chloride containing potassium 0.1-0.3mol/L, 60-80% ethyl alcohol；The magnetic
Rinsing liquid C groups, which become, in pearl method nucleic acid extraction kit contains sodium chloride 0.1-0.3mol/L, 60-80% ethyl alcohol；The magnetic bead
Eluent group, which becomes, in method nucleic acid extraction kit contains 10mol/LTris.HCl (PH8.3), 0.01-0.05%
Prolin300；Magnetic bead liquid group becomes the super suitable nano magnetic of a diameter of 1 μm of silica in the paramagnetic particle method nucleic acid extraction kit
Pearl, a concentration of 10-40mg/ml.
Compared with prior art, the beneficial effects of the invention are as follows：
The configuration of the present invention is simple, it is rationally distributed, first in positive reference substance lattice, detection instrument lattice, negative controls point
Lattice, clean tube lattice and test strip lattice memory put corresponding article, when work, detection instrument are used to acquire blood
To test tube, sample blood is detected using the detection reagent in detection reagent lattice, convenient pair of the clamping device of setting
Detection reagent test tube grips, and can play good buffering effect, securely and reliably；In addition, positive reference substance lattice
Negative controls in interior positive reference substance and negative controls lattice facilitate carry out Comparative result, to facilitate doctor to the state of an illness
Analysis.
Description of the drawings
Fig. 1 is the structural schematic diagram of the infull detection kit of AIDS immunity function restructuring.
Fig. 2 is the sectional view of Section A-A in Fig. 1.
Fig. 3 is the structural schematic diagram of clamping device in the infull detection kit of AIDS immunity function restructuring.
Fig. 4 is the vertical view of lattice closing lid in the infull detection kit of AIDS immunity function restructuring.
In figure：1- box covers, 2- box bodys, 3- handles, 4- cover boards, 5- label boxes, 6- electronic password locks, 7- set up slot, and 8- is put
Set frame, 9- stop sleevees, 10- arc fixture blocks, 11- test tube placed cavities, 12- placing grooves, 13- springs, 14- sliding blocks, 15- compression bars,
16- buffering spacers.
Specific implementation mode
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation describes, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Please refer to Fig.1-4, in the embodiment of the present invention, a kind of infull detection kit of AIDS immunity function restructuring, including
Box cover 1 and box body 2, the box cover 1 are an integral cap, and box cover 1 is connected to by articulated manner on box body 2, the end of box cover 1
Handle 3 is provided on outer wall, the box cover 1 is interior to be separated into multiple lattices, respectively positive reference substance lattice, detection by partition board
Instrument lattice, negative controls lattice, detection reagent lattice, clean tube lattice and test strip lattice, six lattice rows
Front two rows, each three lattices of front two rows are arranged into, multiple cover boards 4 are corresponded with lattice, the top inner wall of each lattice
On offer and be respectively provided with that there are one label box 5, label boxes 5 with 4 matched erection slot 7 of cover board, the top of each cover board 4
It is made of transparent plastic, for placing label, label can mark the article stored in lattice.
It is provided with rack 8 in the detection reagent lattice, test tube placed cavity 11 is offered in rack 8, test tube is placed
The bottom of chamber 11 is fixedly installed placing groove 12, is equipped with for detection reagent in the upper inside wall of the test tube placed cavity 11
The clamping device that test tube is gripped, the clamping device include stop sleeve 9, arc fixture block 10, spring 13, sliding block 14
With compression bar 15, wherein stop sleeve 9 is fixedly mounted in the upper inside wall of test tube placed cavity 11, and the horizontal sliding of sliding block 14 is set to branch
In support set cylinder 9, spring 13 is additionally provided in stop sleeve 9, the side of sliding block 14 is connected with spring 13, the other side of sliding block 14 with
Compression bar 15 is connected, and arc fixture block 10 is fixedly mounted on the other end of compression bar 15；To avoid arc fixture block 10 from being tried with detection reagent
It is had skidded between pipe, it is preferred that the concave side surface of the arc fixture block 10 is provided with buffering spacer 16.
In the embodiment of the present invention, the buffering spacer 16 is made of rubber material.
In the embodiment of the present invention, the box cover 1 is fixed on by electronic password lock 6 on box body 2, and non-permission personnel is avoided to beat
Kit is opened, securely and reliably.
The detection reagent includes PCR reaction reagents, Tag primer Tag, LTR gene-specific primer, GAG gene specifics
Property primer, RNaseP internal control target genes primer, Taq DNA polymerase and PCR reaction solution.
The detection reagent further includes paramagnetic particle method nucleic acid extracting reagent and HIV nucleic acid amplification agents, wherein paramagnetic particle method nucleic acid
Extracts kit includes cracking in conjunction with liquid, rinsing liquid A, rinsing liquid B, rinsing liquid C, eluent, magnetic bead liquid；HIV nucleic acid amplifications try
Agent box includes RT-PCR reaction solutions, enzyme mixation, HIV- internal standards, HIV qualitative reference product 1-4, negative quality-control product, critical positive matter
Control product, strong positive quality-control product.
Cracking combines liquid group to become containing lauryl sodium sulfate 0.8-2.0% in the paramagnetic particle method nucleic acid extracting reagent,
Guanidinium isothiocyanate 3.5-5.5mol/L, 1-10mM/LEDTA (PH7.5)；Rinsing liquid A in the paramagnetic particle method nucleic acid extraction kit
Group, which becomes, contains TritonX-1000.5-2.0ml/100ml, lithium chloride 0.5-1mol/L；The paramagnetic particle method nucleic acid extracting reagent
Rinsing liquid B groups become chloride containing potassium 0.1-0.3mol/L, 60-80% ethyl alcohol in box；In the paramagnetic particle method nucleic acid extraction kit
Rinsing liquid C groups, which become, contains sodium chloride 0.1-0.3mol/L, 60-80% ethyl alcohol；It is washed in the paramagnetic particle method nucleic acid extraction kit
De- liquid group, which becomes, contains 10mol/LTris.HCl (PH8.3), 0.01-0.05%Prolin300；The paramagnetic particle method nucleic acid extraction
Magnetic bead liquid group becomes the super suitable nanometer magnetic bead of a diameter of 1 μm of silica, a concentration of 10-40mg/ml in kit.
The configuration of the present invention is simple, it is rationally distributed, first in positive reference substance lattice, detection instrument lattice, negative controls point
Lattice, clean tube lattice and test strip lattice memory put corresponding article, when work, detection instrument are used to acquire blood
To test tube, sample blood is detected using the detection reagent in detection reagent lattice, convenient pair of the clamping device of setting
Detection reagent test tube grips, and can play good buffering effect, securely and reliably；In addition, positive reference substance lattice
Negative controls in interior positive reference substance and negative controls lattice facilitate carry out Comparative result, to facilitate doctor to the state of an illness
Analysis.
It is obvious to a person skilled in the art that invention is not limited to the details of the above exemplary embodiments, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power
Profit requires rather than above description limits, it is intended that all by what is fallen within the meaning and scope of the equivalent requirements of the claims
Variation is included within the present invention.Any reference signs in the claims should not be construed as limiting the involved claims.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiment being appreciated that.
Claims (9)
1. a kind of infull detection kit of AIDS immunity function restructuring, including box cover (1) and box body (2), which is characterized in that institute
It is an integral cap to state box cover (1), and box cover (1) is connected to by articulated manner on box body (2), in the box cover (1) by every
Plate is separated into multiple lattices, respectively positive reference substance lattice, detection instrument lattice, negative controls lattice, detection reagent point
Lattice, clean tube lattice and test strip lattice, six lattices are arranged in front two rows, and each three lattices of front two rows are more
A cover board (4) corresponds with lattice, is offered in the top inner wall of each lattice and the matched erection slot of cover board (4)
(7)；It is provided with rack (8) in the detection reagent lattice, offers test tube placed cavity (11) in rack (8), test tube is put
The bottom for setting chamber (11) is fixedly installed placing groove (12), be equipped in the upper inside wall of the test tube placed cavity (11) for pair
The clamping device that detection reagent test tube is gripped, the clamping device include stop sleeve (9), arc fixture block (10),
Spring (13), sliding block (14) and compression bar (15), wherein stop sleeve (9) are fixedly mounted on the upper inside wall of test tube placed cavity (11)
On, the horizontal sliding of sliding block (14) is set in stop sleeve (9), is additionally provided with spring (13) in stop sleeve (9), and the one of sliding block (14)
Side is connected with spring (13), and the other side of sliding block (14) is connected with compression bar (15), and arc fixture block (10) is fixedly mounted on pressure
The other end of bar (15).
2. the infull detection kit of a kind of AIDS immunity function restructuring according to claim 1, which is characterized in that described
Handle (3) is provided on the end outer wall of box cover (1).
3. the infull detection kit of a kind of AIDS immunity function restructuring according to claim 1, which is characterized in that described
Label box (5) is both provided at the top of cover board (4).
4. the infull detection kit of a kind of AIDS immunity function restructuring according to claim 3, which is characterized in that described
Label box (5) is made of transparent plastic.
5. the infull detection kit of a kind of AIDS immunity function restructuring according to claim 1, which is characterized in that described
The concave side surface of arc fixture block (10) is provided with buffering spacer (16).
6. the infull detection kit of a kind of AIDS immunity function restructuring according to claim 5, which is characterized in that described
Buffering spacer (16) is made of rubber material.
7. the infull detection kit of a kind of AIDS immunity function restructuring according to claim 1, which is characterized in that described
Box cover (1) is fixed on by electronic password lock (6) on box body (2).
8. the infull detection kit of a kind of AIDS immunity function restructuring according to claim 1, which is characterized in that described
Detection reagent further includes paramagnetic particle method nucleic acid extracting reagent and HIV nucleic acid amplification agents, wherein paramagnetic particle method nucleic acid extraction kit packet
Cracking is included in conjunction with liquid, rinsing liquid A, rinsing liquid B, rinsing liquid C, eluent, magnetic bead liquid；HIV nucleic acid amplification kits include RT-
PCR reaction solution, enzyme mixation, HIV- internal standards, HIV qualitative reference product 1-4, negative quality-control product, critical positive quality control product, strong positive
Quality-control product.
9. the infull detection kit of a kind of AIDS immunity function restructuring according to claim 8, which is characterized in that described
Cracking combines liquid group to become containing lauryl sodium sulfate 0.8-2.0%, guanidinium isothiocyanate in paramagnetic particle method nucleic acid extracting reagent
3.5-5.5mol/L 1-10mM/LEDTA (PH7.5)；Rinsing liquid A groups become and contain in the paramagnetic particle method nucleic acid extraction kit
TritonX-1000.5-2.0ml/100ml, lithium chloride 0.5-1mol/L；Rinsing liquid B in the paramagnetic particle method nucleic acid extraction kit
Group becomes chloride containing potassium 0.1-0.3mol/L, 60-80% ethyl alcohol；Rinsing liquid C is formed in the paramagnetic particle method nucleic acid extraction kit
To contain sodium chloride 0.1-0.3mol/L, 60-80% ethyl alcohol；Eluent group becomes and contains in the paramagnetic particle method nucleic acid extraction kit
There are 10mol/LTris.HCl (PH8.3), 0.01-0.05%Prolin300；Magnetic bead in the paramagnetic particle method nucleic acid extraction kit
Liquid group becomes the super suitable nanometer magnetic bead of a diameter of 1 μm of silica, a concentration of 10-40mg/m.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810294174.1A CN108456636A (en) | 2018-04-04 | 2018-04-04 | A kind of infull detection kit of AIDS immunity function restructuring |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810294174.1A CN108456636A (en) | 2018-04-04 | 2018-04-04 | A kind of infull detection kit of AIDS immunity function restructuring |
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| Publication Number | Publication Date |
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| CN108456636A true CN108456636A (en) | 2018-08-28 |
Family
ID=63234556
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810294174.1A Pending CN108456636A (en) | 2018-04-04 | 2018-04-04 | A kind of infull detection kit of AIDS immunity function restructuring |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111647503A (en) * | 2020-07-09 | 2020-09-11 | 浙江爱津生物技术有限公司 | Magnetic bead method nucleic acid detection kit |
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| CN205672962U (en) * | 2016-06-23 | 2016-11-09 | 张海艳 | Socket unit for test tube rack |
| CN107326102A (en) * | 2017-08-01 | 2017-11-07 | 广西中医药大学附属瑞康医院（广西中西医结合医院） | A kind of AIDS kit |
| CN206715990U (en) * | 2017-05-08 | 2017-12-08 | 四川科泰世纪环保工程有限公司 | A kind of antibody kit rack of HIV rooms |
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| CN110152758A (en) * | 2018-03-30 | 2019-08-23 | 苏州朝霞生物科技有限公司 | A kind of biotechnology exploitation test tube holder device |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001042496A2 (en) * | 1999-12-10 | 2001-06-14 | Pyrosequencing Ab | A method of assessing the amount of nucleic acid in a sample |
| US20050154189A1 (en) * | 2000-06-23 | 2005-07-14 | Maxygen Inc | Novel co-stimulatory molecules |
| CN204964509U (en) * | 2015-10-09 | 2016-01-13 | 中国人民解放军济南军区联勤部疾病预防控制中心 | AIDS detects and prevention kit |
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| CN206715990U (en) * | 2017-05-08 | 2017-12-08 | 四川科泰世纪环保工程有限公司 | A kind of antibody kit rack of HIV rooms |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111647503A (en) * | 2020-07-09 | 2020-09-11 | 浙江爱津生物技术有限公司 | Magnetic bead method nucleic acid detection kit |
| CN111647503B (en) * | 2020-07-09 | 2021-05-25 | 浙江爱津生物技术有限公司 | Magnetic bead method nucleic acid detection kit |
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Application publication date: 20180828 |