CN218710457U

CN218710457U - Portable extracting tool box for oncomelania nucleic acid

Info

Publication number: CN218710457U
Application number: CN202221328649.2U
Authority: CN
Inventors: 周晓蓉; 朱红; 易佳; 孙凌聪; 董小蓉
Original assignee: HUBEI PROVINCIAL CENTER FOR DISEASE CONTROL AND PREVENTION
Current assignee: HUBEI PROVINCIAL CENTER FOR DISEASE CONTROL AND PREVENTION
Priority date: 2022-05-30
Filing date: 2022-05-30
Publication date: 2023-03-24
Anticipated expiration: 2032-05-30

Abstract

The application discloses portable extraction tool holder of oncomelania nucleic acid, inside including of portable extraction tool holder: the device comprises a clamping device, a sampling device, a plurality of centrifuge tubes, a collecting tube, a nucleic acid extraction device and a nucleic acid extraction reagent; wherein at least one of the plurality of centrifuge tubes comprises an inner tube to which an adsorption membrane is attached; the inner tube is configured to absorb a nucleic acid extraction reagent transferred from a nucleic acid extraction device to the adsorption membrane; the collection tube is configured to contain the extracted nucleic acid solution; the volume of the portable extraction tool box is amm x b mm x h mm, wherein a is greater than or equal to 16mm and less than or equal to 26mm, b is greater than or equal to 20mm and less than or equal to 32mm, and h is greater than or equal to 16mm and less than or equal to 28mm. The method can obtain pure nucleic acid solution for subsequent amplification experiments, can detect 50 oncomelania at one time, can enlarge efficiency, can solve the problem that a high-speed centrifuge is not needed in field application, and has an application prospect of field batch screening.

Description

Portable extracting tool box for oncomelania nucleic acid

Technical Field

The application relates to the field of biological detection, in particular to a portable extraction tool box for oncomelania nucleic acid.

Background

Schistosomiasis is a parasitic disease which is caused by the infection of human or animals with schistosomiasis, seriously harms human health and the development of economy and society, and is one of the important public health problems in the world. Oncomelania snail is the intermediate host of schistosomiasis and is the transmission medium of schistosomiasis. While snail distribution monitoring is one of the most important loops in propagation risk monitoring and control. Traditional oncomelania monitoring is based on anatomical microscopic observation of schistosoma japonicum cercaria. But the actual infection in the epidemic environment is often underestimated due to the low sensitivity of the detection method. Nucleic acid-based molecular biology methods (such as PCR and LAMP) have the advantages of high sensitivity and good specificity, and have begun to be applied to the monitoring of infectious oncomelania in schistosomiasis epidemic areas. The nucleic acid extraction of the oncomelania is the first link of molecular biological detection. At present, a common oncomelania nucleic acid extraction method is a microcentrifuge column method, but the method has small detection quantity, needs a high-speed centrifuge and is not suitable for field batch screening.

Disclosure of Invention

The portable extraction tool box for the nucleic acid of the oncomelania aims to provide a pure nucleic acid solution for a subsequent amplification experiment after steps of cracking treatment, filtering, syringe suction, nucleic acid adsorption of a silicon substrate membrane, rinsing of the silicon substrate membrane by a rinsing solution, nucleic acid elution of an eluent and the like. The method can detect 50 oncomelania at one time, not only can enlarge the efficiency, but also can solve the problem that a high-speed centrifuge is not needed in field application, and has an application prospect of field batch screening.

The application discloses portable extraction tool box of oncomelania nucleic acid, portable extraction tool box inside includes: the device comprises a clamping device, a sampling device, a plurality of centrifuge tubes, a collecting tube, a nucleic acid extraction device and a nucleic acid extraction reagent;

wherein at least one of the plurality of centrifuge tubes comprises an inner tube to which an adsorption membrane is attached; the inner tube is configured to absorb a nucleic acid extraction reagent transferred from a nucleic acid extraction device to the adsorption membrane;

the collection tube is configured to contain the extracted nucleic acid solution;

the volume of the portable extraction tool box is a mm multiplied by b mm multiplied by h mm, wherein a is greater than or equal to 16mm and less than or equal to 26mm, b is greater than or equal to 20mm and less than or equal to 32mm, and h is greater than or equal to 16mm and less than or equal to 28mm.

In a preferred embodiment, the grasping device is a forceps.

In a preferred embodiment, the sampling device is a glass plate.

In a preferred example, the nucleic acid extraction device is a syringe, and the nucleic acid extraction reagent is cell lysate, rinsing solution and eluent.

In a preferred embodiment, the adsorption film is a silicon-containing matrix film.

In a preferred embodiment, the test tube rack is further included.

In a preferred embodiment, the fabric further comprises gauze.

In a preferred embodiment, the centrifuge tube has a volume of 50ml.

In a preferred embodiment, the volume of the collection tube is 2.0ml.

The application has at least the following technical effects:

1. the kit can detect the schistosoma japonicum nucleic acid fragment formed by mixing the positive and negative oncomelania, has detection value and is expected to be used for detecting a schistosoma infected intermediate host;

2. the utility model provides a toolbox once can detect 50 oncomelania, greatly improves detection efficiency, need not special instrument and equipment (such as fluorescence detection appearance, electrophoresis apparatus, electrochemistry DNA sensor etc.), need not high speed centrifuge, and the reaction is quick, but the visual observation result has stronger schistosomiasis prevention and cure field application potentiality, has the application prospect at on-the-spot screening in batches.

The present specification describes a number of technical features distributed throughout the various technical aspects, and if all possible combinations of technical features (i.e. technical aspects) of the present specification are listed, the description is made excessively long. In order to avoid this problem, the respective technical features disclosed in the above summary of the invention of the present application, the respective technical features disclosed in the following embodiments and examples, and the respective technical features disclosed in the drawings may be freely combined with each other to constitute various new technical solutions (which should be regarded as having been described in the present specification) unless such a combination of the technical features is technically infeasible. For example, in one example, the feature a + B + C is disclosed, in another example, the feature a + B + D + E is disclosed, and the features C and D are equivalent technical means for the same purpose, and technically only one feature is used, but not simultaneously employed, and the feature E can be technically combined with the feature C, then the solution of a + B + C + D should not be considered as being described because the technology is not feasible, and the solution of a + B + C + E should be considered as being described.

Drawings

Fig. 1 is a schematic view of a portable extraction kit according to the present application.

Description of the reference numerals

1-a gripping device; 2-a sampling device; 3-a test tube rack; 4-gauze; 5-grinding rod; 6-centrifuging the tube; 7-a syringe; 8-collecting tube.

Detailed Description

In the following description, numerous technical details are set forth in order to provide a better understanding of the present application. However, it will be understood by those skilled in the art that the technical solutions claimed in the present application may be implemented without these technical details and with various changes and modifications based on the following embodiments.

The following outlines some of the innovative points of the embodiments of the present application:

the utility model provides a portable instrument box that draws of oncomelania nucleic acid, including tweezers, glass board, test-tube rack, a plurality of centrifuging tube, collecting pipe, nucleic acid extraction element and nucleic acid extraction reagent. Wherein, centrifuging tube includes an inner tube, and the bottom is attached to the adsorption film. The adsorption film is preferably a silicon-containing matrix film. The centrifuge tube also comprises a pressing ring and a sieve plate. The centrifuge tube had a volume of 50ml. The volume of the collection tube was 2.0ml. The volume of the portable extraction tool box is a mm multiplied by b mm multiplied by h mm, wherein a is larger than or equal to 16mm and smaller than or equal to 26mm, b is larger than or equal to 20mm and smaller than or equal to 32mm, and h is larger than or equal to 16mm and smaller than or equal to 28mm. In a preferred embodiment, the volume of the kit is 24 x 30 x 24mm ³ 。

The nucleic acid extraction reagent comprises:

(1) Tissue/cell lysate: 10mmol/L Tris-HCl (pH 8.0), 100mmol/L EDTA, 5g/L Sodium Dodecyl Sulfate (SDS), 100mg RNase A, proteinase K solution (20 m g/mL).

(2) Rinsing liquid:

0.1mmol/L NaCl

10mmol/LTris-HCl(pH8.0)

1mmol/L EDTA(pH8.0)

20% ethanol (final concentration)

(3) Eluent solution

10mmol/LTris HCl(pH8.0)

1mmol/L EDTA(pH8.0)

To make the objects, technical solutions and advantages of the present application more clear, embodiments of the present application will be described in further detail below with reference to the accompanying drawings.

The embodiment of using the portable extraction tool cartridge of the present application to extract oncomelania nucleic acid is as follows:

(1) Crushing the snails on a glass plate, removing the shell, picking the meat tissues of the snails to a 50ml centrifuge tube by using forceps, and putting 50 tissues of the snails to 1 tube;

(2) Adding 5ml of tissue/cell lysate to soak the snail meat tissue, grinding for 2 minutes by using a grinding rod, and then putting the ground snail meat tissue into a 56 ℃ water bath for cracking for 2 hours;

(3) Filtering the cracked lysate with sterile gauze to a 50ml centrifuge tube, extracting liquid to an inner tube with an adsorption membrane with an injector, standing at room temperature for 1min, pressing the liquid with a sample adding needle, and discarding the liquid;

(4) Extracting 5ml of rinsing liquid into the inner tube of the adsorption membrane by using an injector, standing for 1min at room temperature, pressing the liquid by using a sampling needle, and discarding the liquid; airing for 10 minutes at room temperature until the ethanol is completely volatilized;

(5) 0.5ml of the eluate was aspirated by a syringe, allowed to stand at room temperature for 5min, and the liquid was pressed down into a 2ml collection tube with a sample-adding needle.

It is noted that, in the present patent application, relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, the use of the verb "comprise a" to define an element does not exclude the presence of another, same element in a process, method, article, or apparatus that comprises the element. In the present patent application, if it is mentioned that a certain action is executed according to a certain element, it means that the action is executed according to at least the element, and two cases are included: performing the action based only on the element, and performing the action based on the element and other elements. The expression of a plurality of, a plurality of and the like includes 2, 2 and more than 2, more than 2 and more than 2.

This specification includes combinations of the various embodiments described herein. Separate references to "one embodiment" or a particular embodiment, etc., do not necessarily refer to the same embodiment; however, these embodiments are not mutually exclusive, unless indicated as mutually exclusive or as would be apparent to one of ordinary skill in the art. It should be noted that the term "or" is used in this specification in a non-exclusive sense unless the context clearly dictates otherwise.

All documents mentioned in this application are to be considered as being incorporated in their entirety into the disclosure of this application so as to be subject to modification as necessary. Further, it is understood that various changes or modifications may be made to the present application by those skilled in the art after reading the above disclosure of the present application, and such equivalents are also within the scope of the present application as claimed.

Claims

1. A portable extraction tool kit for oncomelania nucleic acid, comprising, inside: the device comprises a clamping device, a sampling device, a plurality of centrifuge tubes, a collecting tube, a nucleic acid extraction device and a nucleic acid extraction reagent;

2. The portable extraction tool kit of claim 1, wherein said grasping device is a forceps.

3. The portable extraction tool kit of claim 1, wherein said sampling device is a glass plate.

4. The portable extraction kit of claim 1, wherein the nucleic acid extraction device is a syringe.

5. The portable extraction tool kit of claim 1, wherein said adsorbent film is a silicon-containing matrix film.

6. The portable extraction tool kit of claim 1, further comprising a test tube rack.

7. The portable extraction kit of claim 1, further comprising gauze.

8. The portable extraction tool kit of claim 1, wherein the centrifuge tube has a volume of 50ml.

9. The portable extraction tool kit of claim 1, wherein said collection tube has a volume of 2.0ml.