CN110628569A

CN110628569A - Full-automatic nucleic acid extraction system

Info

Publication number: CN110628569A
Application number: CN201910982697.XA
Authority: CN
Inventors: 邹继华; 谢召杰; 朱磊; 徐勇
Original assignee: NINGBO MEIKANG SHENGDE BIOTECHNOLOGY CO Ltd
Current assignee: NINGBO MEIKANG SHENGDE BIOTECHNOLOGY CO Ltd; Ningbo Medical System Biotechnology Co Ltd
Priority date: 2019-10-16
Filing date: 2019-10-16
Publication date: 2019-12-31

Abstract

The invention relates to the field of biotechnology equipment, and provides a full-automatic nucleic acid extraction system which comprises a mounting seat, an injector component, an extraction platform component, a magnetic separation component, a kit and a driving component, the injector assembly and the extraction platform assembly are both connected with the mounting seat in a sliding way, the injector assembly is positioned above the extraction platform assembly, the kit is carried on the extraction platform assembly, the driving assembly drives the extraction platform assembly to move back and forth and complete the operation and positioning actions, the magnetic separation component is arranged on the extraction platform component and moves along with the extraction platform component, the driving assembly drives the injector assembly to move up and down and complete actions of puncturing the packaging film, taking/taking off the liquid transfer head or sucking/discharging liquid, and the driving assembly drives the magnetic separation assembly to move up and down and complete actions of magnetic bead separation. The full-automatic nucleic acid extraction system has the advantages of accurate suction and discharge liquid control, short experimental process time and capability of fully ensuring the validity of experimental results.

Description

Full-automatic nucleic acid extraction system

Technical Field

The invention relates to the field of biotechnology equipment, in particular to a full-automatic nucleic acid extraction system.

Background

In the fields of genetic engineering and protein engineering technology research, nucleic acid molecules are the main research objects, and since nucleic acids exist in cells in a state of being bound to proteins, nucleic acid extraction is a basic technology for nucleic acid research as a pretreatment work for nucleic acid analysis. The purpose of nucleic acid extraction is to remove protein, polysaccharide, lipid and other biological macromolecules combined with nucleic acid, and the current main nucleic acid extraction method is a magnetic bead method, wherein magnetic beads, namely magnetic particles for nucleic acid separation, need to have two characteristics of superparamagnetism and surface functional groups. Firstly, superparamagnetism ensures that the aggregation and dispersion of magnetic beads can be controlled by an external magnetic field; secondly, the functional groups on the surface of the magnetic beads react with nucleic acid molecules under certain conditions to enrich the nucleic acid. The extraction of nucleic acid by magnetic bead method mainly comprises three processes: firstly, combining nucleic acid molecules with magnetic beads to form magnetic bead-nucleic acid complexes; secondly, separating the magnetic bead-nucleic acid compound from the solution under the action of an external magnetic field; thirdly, eluting nucleic acid. In the magnetic bead method, the liquid-transfer type nucleic acid extraction is widely used, namely, the extraction and purification of nucleic acid are realized by transferring a reaction solution, and the steps comprise: adding lysis solution into the sample, blowing, beating and mixing uniformly, separating nucleic acid from biomacromolecules such as protein and the like, and then repeatedly performing operations of magnetic bead adsorption, removing lysis solution, adding washing solution to wash the magnetic beads, adsorbing the magnetic beads, removing the washing solution, adding elution buffer solution and the like, thereby obtaining the purified nucleic acid.

In order to reduce experimental errors, improve the repeatability and reliability of the experiment and improve the experimental efficiency so as to meet the requirement of batch extraction of samples which is rapidly developed at present, the traditional manual experiment for nucleic acid extraction is replaced by a full-automatic nucleic acid extractor. For rapid detection with low flux requirement, the small-sized full-automatic nucleic acid extractor can be matched with a packaged kit to rapidly complete extraction and purification of nucleic acid, the equipment has low running cost and convenient operation, has larger requirements and application prospects, and is increasingly applied to laboratories, hospitals and the like. The nucleic acid extraction requires accurate positioning and fine operation in the operation process, the full-automatic nucleic acid extraction instrument is required to be a precise instrument, the matching among all transmission connecting parts is required to be precise and meticulous, and the transmission precision can be obviously reduced by adopting flexible parts such as springs and the like to connect transmission parts in some nucleic acid extraction instruments on the market at present. As continuous technical improvement, the internal structure of the full-automatic nucleic acid extractor needs to be further simplified, the reliability and consistency of transmission are enhanced, the experimental flow time is shortened as much as possible on the premise of improving the system integration level, the process pollution is reduced, and the effectiveness of the experimental result is ensured.

Disclosure of Invention

The invention aims to solve the technical problem of providing a full-automatic nucleic acid extraction system which is composed of a full-automatic nucleic acid extractor and a kit which is packaged in a matched manner, wherein the full-automatic nucleic acid extractor has a simple internal structure, the matching among transmission connecting parts is strict and delicate, the whole full-automatic nucleic acid extractor system has high integration level, short experimental process time and low process pollution probability, and can quickly and effectively complete the extraction and purification of nucleic acid.

The invention provides a full-automatic nucleic acid extraction system, which comprises a mounting seat, an injector assembly, an extraction platform assembly, a magnetic separation assembly, a kit and a driving assembly, wherein the injector assembly and the extraction platform assembly are both mounted on the mounting seat, the injector assembly is positioned above the extraction platform assembly, the kit is carried on the extraction platform assembly, and the driving assembly drives the extraction platform assembly to move back and forth so that the injector assembly is aligned with an operation position on the extraction platform assembly; the magnetic separation assembly is arranged on the extraction platform assembly and moves along with the extraction platform assembly, and the driving assembly drives the magnetic separation assembly to move up and down so that the magnetic separation assembly performs magnetic bead separation on the solution in the reagent kit; the driving component drives the injector component to move up and down, so that the injector component punctures an encapsulation film on the reagent box, or the injector component takes/takes off a liquid-moving head on the extraction platform component, or the injector component sucks/discharges reagents in the reagent box.

Compared with the prior art, the full-automatic nucleic acid extraction system has the following advantages:

1. in the full-automatic nucleic acid extraction system, the injector assembly, the magnetic separation assembly and the extraction platform assembly carrying the kit are fully matched, so that the actions of operation positioning, puncturing the packaging film, taking/removing the pipette head, sucking/discharging the reagent and separating the magnetic beads can be completed, the system integration level is high, the experimental flow time is short, the process pollution probability is low, and the extraction and purification of the nucleic acid can be quickly and effectively completed;

2. in the full-automatic nucleic acid extraction system, each motion module only moves in a single direction, the motion control is simple, the phenomenon that the integral transmission precision is greatly reduced due to the accumulation of motion errors generated by the same module in multiple directions is avoided, the motion amplitude is accurately controlled, and the liquid suction and discharge of an injection assembly can be accurately controlled;

3. in the full-automatic nucleic acid extraction system, the injector assembly, the magnetic separation assembly and the extraction platform assembly carrying the kit are all modularized, and can be expanded according to experimental needs to improve flux, so that the equipment manufacturing difficulty is low, and the operation cost is low.

Preferably, the injector assembly comprises a fixing part, an injector driving part, at least one group of piston rods and an injection sleeve, the injector driving part, the piston rods and the injection sleeve are all arranged on the fixing part, the lower end of each piston rod is provided with a puncture tip, and the injector driving part comprises a piston driving part and an injection sleeve driving part; the injection sleeve driving part drives the injection sleeve to move downwards, so that the injection sleeve is worn on the liquid transferring head on the extraction platform assembly; the piston driving piece drives the piston rod to move downwards, so that the puncture tip punctures an encapsulation film on the kit, or the piston rod pushes a liquid transfer head on the injection sleeve until the liquid transfer head falls off; the piston driving piece drives the piston rod to move up and down, so that the injector sucks/discharges the reagent in the reagent box. By adopting the structure, the injector assembly can complete the actions of puncturing the packaging film, taking/taking off the liquid transferring head and sucking/discharging the reagent, and the lower end of the piston rod is provided with the puncturing tip, so that the puncturing component is reduced, and the cost is saved.

Preferably, the fixing member comprises a first fixing plate, a second fixing plate, a piston rod driving plate and an injection sleeve fixing plate, the first fixing plate and the injection sleeve fixing plate are respectively and fixedly installed at the upper end and the lower end of the second fixing plate, a limiting hole corresponding to the injector is formed in the injection sleeve fixing plate, the injection sleeve is fixed in the limiting hole, the upper end of the piston rod is fixed on the piston rod driving plate, and the lower end of the piston rod penetrates through the injection sleeve fixing plate and is inserted into the injection sleeve; the injection sleeve driving piece drives the second fixing plate to move up and down, so that the injection sleeve fixing plate drives the injection sleeve to move up and down; the piston driving piece drives the piston rod driving plate to move up and down, so that the piston rod moves up and down. By adopting the structure, a single injection sleeve driving part can simultaneously drive a plurality of injection sleeves to move up and down, and a single piston driving part can simultaneously drive a plurality of piston rods to move up and down.

Preferably, the injector assembly comprises a fixing part, and an injector consisting of a puncture needle, an injector driving part, at least one group of piston rods and an injection sleeve, wherein the puncture needle, the injector driving part, the at least one group of piston rods and the injection sleeve are arranged on the fixing part; the puncture driving piece drives the puncture needle to move up and down, so that the puncture needle punctures the packaging film on the kit; the pipetting head taking-off driving part drives the injection sleeve to move up and down, so that the injection sleeve takes off the pipetting head on the extraction platform assembly; the liquid suction and discharge driving part drives the piston rod to move up and down, so that the injector performs suction/discharge actions on the reagent in the reagent box. By adopting the structure, the single puncture needle is adopted, and the reagent is prevented from being polluted.

Preferably, the fixing member comprises a first fixing plate, a second fixing plate, a piston rod driving plate, an injection sleeve driving plate and an injector clamping plate, the first fixing plate and the injector clamping plate are respectively and fixedly arranged at the upper end and the lower end of the second fixing plate, a clamping groove corresponding to the injector is formed in the injector clamping plate, the upper end of the injection sleeve is fixed on the injection sleeve driving plate, the lower part of the injection sleeve is limited in the clamping groove and can slide in the clamping groove, the upper end of the piston rod is fixed on the piston rod driving plate, the lower end of the piston rod penetrates through the injection sleeve driving plate and is inserted into the injection sleeve, and the puncture needle is arranged on the lower; the pipetting head taking-off driving piece drives the injection sleeve driving plate to move up and down so as to enable the injection sleeve to move up and down; the liquid suction and discharge driving part drives the piston rod driving plate to move up and down, so that the piston rod moves up and down; the puncture driving piece drives the second fixing plate to move up and down, so that the syringe clamping plate drives the puncture needle to move up and down. By adopting the structure, the single pipetting head taking and taking-off driving part can simultaneously drive the plurality of injection sleeves to move up and down to complete the action of taking/taking off the pipetting head, the single pipetting driving part can simultaneously drive the plurality of piston rods to move up and down to suck/discharge the reagent, and the single puncture driving part can simultaneously drive the plurality of puncture needles to puncture the packaging film.

Preferably, the magnetic separation assembly comprises a fifth fixing plate, a magnet mounting seat and a magnetic separation driving piece, the magnetic separation assembly is mounted on the extraction platform assembly through the fifth fixing plate, the magnetic separation driving piece is mounted on the fifth fixing plate, and a plurality of magnet blocks are mounted on the magnet mounting seat; the magnetic separation driving part drives the magnet mounting seat to move up and down, so that the magnet block is close to or far away from the magnetic bead solution in the reagent box to perform magnetic bead separation. By adopting the structure, the magnetic separation assembly can simultaneously separate magnetic beads in a plurality of magnetic bead separation cups, and the operation is simple and reliable.

Preferably, the magnetic separation driving part comprises a motor kit and a rotating shaft, at least one cam is installed on the rotating shaft, the motor kit drives the rotating shaft to rotate, the rotating shaft drives the cam to rotate, and the cam drives the magnet installation seat to move up and down. By adopting the structure, the magnet mounting seat can move up and down stably.

Preferably, the magnetic separation driving part comprises a screw motor, a screw nut is correspondingly installed on the magnet installation seat, and the screw nut is in threaded connection with a transmission screw of the screw motor; the screw motor drives the transmission screw to rotate, so that the screw nut drives the magnet mounting seat to move up and down along the transmission screw. With this structure, the structure of the magnetic separation assembly is further simplified.

Preferably, the extraction platform assembly is provided with a product area, a pipetting area, a sample area and a kit area for carrying a kit, the product area is used for placing a final product of nucleic acid extraction, the pipetting area is used for placing a pipetting head, the sample area is used for placing a sample to be tested, the kit is provided with an incubation area, a reagent area and a magnetic bead separation area, the incubation area is used for processing a reaction needing to be heated, the reagent area is used for placing various reagents needed by the test, and the magnetic bead separation area is used for processing the operation of magnetic bead separation. By adopting the structure, the function of each operation area on the extraction platform assembly is clearly distinguished, and when nucleic acid is extracted from a sample, confusion is avoided, and the experiment process is convenient to control.

Preferably, the fully automatic nucleic acid extraction system of the present invention further comprises a control module and a heating rod, wherein the heating rod is located below the incubation region, the control module is connected to the driving assembly and the heating rod, respectively, and the control module is used for controlling the progress of the nucleic acid extraction experiment. By adopting the structure, the full-automatic nucleic acid extraction system can realize automation for extracting nucleic acid from a sample, and avoids manual operation.

Drawings

FIG. 1 is a front view of the internal mechanical structure of a first embodiment of the fully automatic nucleic acid isolation system of the present invention.

FIG. 2 is a rear view of the internal mechanical structure of the first embodiment of the fully automatic nucleic acid isolation system of the present invention.

FIG. 3 is a schematic diagram of an injector assembly in the first embodiment of the fully automatic nucleic acid extraction system of the present invention.

FIG. 4 is a schematic structural diagram of a syringe with a pipetting head according to an embodiment of the fully automatic nucleic acid extraction system of the present invention.

FIG. 5 is a cross-sectional view of a syringe with a pipetting head according to an embodiment of the automatic nucleic acid extracting system of the present invention.

FIG. 6 is a cross-sectional view of a syringe without a pipetting head according to an embodiment of the automatic nucleic acid isolation system of the present invention.

FIG. 7 is a schematic structural diagram of a magnetic separation assembly in a first embodiment of the fully automatic nucleic acid extraction system of the present invention.

FIG. 8 is a schematic view of the internal mechanical structure of a second embodiment of the fully automatic nucleic acid isolation system according to the present invention.

FIG. 9 is a schematic diagram of the injector assembly of the second embodiment of the fully automatic nucleic acid extraction system of the present invention.

FIG. 10 is a left side view of the injector assembly in the second embodiment of the fully automatic nucleic acid extraction system of the present invention.

FIG. 11 is a schematic structural diagram of a magnetic separation assembly in a third embodiment of the fully automatic nucleic acid extraction system of the present invention.

FIG. 12 is a schematic diagram of the structure of the reagent cartridge area of the fully automatic nucleic acid extraction system of the present invention.

As shown in the figure: 1. the injection device comprises an injector assembly, 1-1, a first fixing plate, 1-2, a second fixing plate, 1-3, a piston rod driving plate, 1-4, an injection sleeve fixing plate, 1-5, a sealing nut, 1-6, a piston rod, 1-7, an injection sleeve, 1-8, a first guide shaft, 1-9, a puncture tip, 1-10, a first linear bearing, 1-11, an upper sealing ring, 1-12, a lower sealing ring, 1-13, a first step surface, 1-14, a second step surface, 1-15, a piston rod driving plate, 1-16, an injection sleeve driving plate, 1-17, an injector clamping plate, 1-18, a puncture needle, 2, an extraction platform assembly, 2-1, a product area, 2-2, a pipetting area, 2-4 and a sample area, 2-5 parts of a kit region, 2-51 parts of an incubation region, 2-52 parts of a reagent region, 2-53 parts of a magnetic bead separation region, 3 parts of an injector driving component, 3-1 parts of a first screw rod motor, 3-2 parts of a first guide rail, 3-3 parts of a second guide rail, 3-4 parts of a first induction sheet, 3-5 parts of an electric lead, 3-6 parts of a sliding block, 4 parts of a piston driving component, 4-1 parts of a second screw rod motor, 4-2 parts of a second screw rod nut, 5 parts of a liquid-transferring head, 5-1 parts of a filter core, 6 parts of an extraction platform driving component, 6-1 parts of a third screw rod motor, 6-2 parts of a sliding part, 6-3 parts of a third guide rail, 6-4 parts of a fourth guide rail, 6-5 parts of a first translation plate, 6-6 parts of a second translation plate, 6-7 parts of a stabilizing frame, 6-8 parts of a screw rod bracket, 6-9 parts of a fourth fixing plate, 6-10 parts of a cable protection chain, 7 parts of a mounting seat, 7-1 parts of a first upright post, 7-2 parts of a second upright post, 7-3 parts of a third fixing plate, 7-4 parts of a vertical plate, 7-5 parts of a bottom plate, 7-6 parts of a support leg, 8 parts of a magnetic separation component, 8-1 parts of a motor kit, 8-2 parts of a magnet mounting seat, 8-3 parts of a magnet mounting groove, 8-4 parts of a magnet block, 8-5 parts of a second guide shaft, 8-6 parts of a second linear bearing, 8-7 parts of a translation supporting plate, 8-8 parts of a connecting plate, 8-9 parts of a rotating shaft supporting seat, 8-10 parts of a rotating shaft, 8-11 parts of a first cam, 8-12 parts of a second cam, 8-13 parts of a fifth fixing, 8-14 parts of a second guide shaft mounting plate, 8-15 parts of a motor fixing seat, 8-16 parts of a third optical coupler, 8-17 parts of a third induction sheet, 8-18 parts of a fourth screw motor, 8-19 parts of a fourth screw nut, 8-20 parts of a screw connecting bottom plate, 9 parts of a puncture driving assembly, 10 parts of a pipetting head taking and releasing driving assembly, 10-1 parts of a fifth screw motor, 10-2 parts of a fifth screw nut, 11 parts of a pipetting liquid driving assembly, 11-1 parts of a sixth screw motor, 11-2 parts of a sixth screw nut, 11-3 parts of a sixth fixing plate.

1 ', an injector assembly, 1-1', a first fixing plate, 1-2 ', a second fixing plate, 1-6', a piston rod, 1-7 ', an injection sleeve, 1-8' and a first guide shaft.

Detailed Description

For a better understanding of the present application, various aspects of the present application will be described in more detail with reference to the accompanying drawings. It should be understood that the detailed description is merely illustrative of exemplary embodiments of the present application and does not limit the scope of the present application in any way. Like reference numerals refer to like elements throughout the specification.

In the drawings, the thickness, size, and shape of an object have been slightly exaggerated for convenience of explanation. The figures are purely diagrammatic and not drawn to scale.

It will be further understood that the terms "comprises," "comprising," "includes," "including," "has," "having," "contains," "including," and/or "containing," when used in this specification, specify the presence of stated features, integers, steps, operations, elements, and/or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, and/or groups thereof. Further, when a statement such as "… at least one" appears after the list of listed features, the entire listed feature is modified rather than modifying individual elements in the list.

As shown in FIGS. 1 and 2, the internal mechanical structure of the first embodiment of the fully automatic nucleic acid extracting system of the present invention comprises a syringe assembly 1, a syringe driving assembly 3, a piston driving assembly 4, an extracting platform driving assembly 6, a mounting seat 7, and two sets of extracting platform assemblies 2. The mounting base 7 comprises a first upright post 7-1, a second upright post 7-2, a third fixing plate 7-3, a vertical plate 7-4 and a bottom plate 7-5, the first upright post 7-1 and the second upright post 7-2 are mounted on the bottom plate 7-5, the third fixing plate 7-3 is mounted at the tops of the first upright post 7-1 and the second upright post 7-2, the vertical plate 7-4 is mounted on the first upright post 7-1, the second upright post 7-2 and the third fixing plate 7-3, four support legs 7-6 are further mounted at the bottom of the bottom plate 7-5, and the injector assembly 1 is mounted on the vertical plate 7-4. The extraction platform driving assembly 6 comprises a third screw rod motor 6-1, a sliding part 6-2, a third guide rail 6-3, a fourth guide rail 6-4, a first translation plate 6-5, a second translation plate 6-6, a stabilizing frame 6-7, a screw rod bracket 6-8 and a fourth fixing plate 6-9, wherein the third screw rod motor 6-1, the third guide rail 6-3, the fourth guide rail 6-4 and the screw rod bracket 6-8 are all arranged on a bottom plate 7-5, the first translation plate 6-5 is in sliding connection with the third guide rail 6-3, the second translation plate 6-6 is in sliding connection with the fourth guide rail 6-4, the fourth fixing plate 6-9 is arranged above the first translation plate 6-5 and the second translation plate 6-6, the stabilizing frame 6-7 is supported below the fourth fixing plate 6-9, two groups of extraction platform assemblies 2 are fixedly arranged on a fourth fixing plate 6-9, the other end of a screw rod of a third screw rod motor 6-1 is arranged in a bearing hole of a screw rod bracket 6-8 through a bearing, a sliding part 6-2 is in threaded connection with the screw rod of the third screw rod motor 6-1, the sliding part 6-2 is also fixedly connected with a first translation plate 6-5, two optocouplers (not shown) for detecting the position information of the sliding part 6-2 are also arranged on a bottom plate 7-5, an induction sheet (not shown) is arranged on the side surface of the sliding part 6-2, the two optocouplers respectively correspond to two ends of the screw rod of the third screw rod motor 6-1, and the optocouplers generate an electric signal related to the position of the sliding part 6-2 by collecting the position information of the induction sheet. The injector driving assembly 3 comprises a first screw rod motor 3-1, a first guide rail 3-2, a second guide rail 3-3 and a slide block (not shown in the figure), the first screw rod motor 3-1 is arranged on a third fixing plate 7-3, the first guide rail 3-2 and the second guide rail 3-3 are arranged on a vertical plate 7-4, the slide block is in threaded connection (not shown in the figure) with a screw rod of the first screw rod motor 3-1 and is in sliding connection with the first guide rail 3-2 and the second guide rail 3-3, two optocouplers for detecting the position information of the injector assembly 3 are also arranged on the vertical plate 7-4, the two optocouplers are positioned at the same side of the injector assembly and respectively correspond to the upper end and the lower end of the first guide rail 3-2, a first induction sheet 3-4 is arranged on one side of the second fixing plate 1-2 of the injector assembly 3 corresponding, the optical coupler generates an electric signal about the position of the injector assembly 3 by collecting position information of the first sensing piece 3-4. The third screw motor 6-1 drives the sliding part 6-2 to move back and forth, the sliding part 6-2 drives the first translation plate 6-5 and the second translation plate 6-6 to respectively slide back and forth on the third guide rail 6-3 and the fourth guide rail 6-4, so that the fourth fixing plate 6-9 with the two groups of extraction platform assemblies 2 thereon also move back and forth.

As shown in FIG. 3 to FIG. 6, the first embodiment of the fully automatic nucleic acid extracting system of the present invention comprises a set of syringe assemblies 1, wherein the syringe assemblies 1 comprise a first fixing plate 1-1, a second fixing plate 1-2, a piston rod driving plate 1-3, a syringe sleeve fixing plate 1-4, 16 piston rods 1-6, 16 syringe sleeves 1-7, 2 first guide shafts 1-8, and 2 first linear bearings 1-10; the second fixing plate 1-2 is fixed on a sliding block in the injector driving component 3 and slides up and down along the first guide rail 3-2 and the second guide rail 3-3 along with the sliding block; the first fixing plate 1-1 and the injection sleeve fixing plate 1-4 are respectively and fixedly arranged at the upper end and the lower end of the second fixing plate 1-2, the upper end and the lower end of the first guide shaft 1-8 are respectively and fixedly arranged on the first fixing plate 1-1 and the injection sleeve fixing plate 1-4, the first linear bearing 1-10 is fixed on the piston rod driving plate 1-3, and the first guide shaft 1-8 is in sliding connection with the piston rod driving plate 1-3 through the first linear bearing 1-10; the outer wall of the injection sleeve 1-7 is provided with a first step surface 1-13, the injection sleeve 1-7 is divided into two parts with large cross section areas and small cross section areas by the first step surface 1-13, a limit hole corresponding to the quantity and the position of an injector is arranged on an injection sleeve fixing plate 1-4, the size of the limit hole is between the cross section sizes of the upper part and the lower part of the injection sleeve 1-7, the lower part of the injection sleeve 1-7 passes through the limit hole, the first step surface 1-13 is limited above the limit hole, the outer wall of the lower part of the injection sleeve 1-7 is also provided with an upper ring limit boss and a lower ring limit boss, the side surface of the injection sleeve fixing plate 1-4 corresponding to the position of the limit hole is also provided with a screw hole, a screw is screwed into the screw hole, so that the screw is limited between the upper ring limit boss and the lower ring limit boss, thus, the injection sleeve 1-7 is fixed in the limiting hole of the injection sleeve fixing plate 1-4; the piston rod 1-6 can slide up and down in the injection sleeve 1-7, the upper end of the piston rod 1-6 is provided with a threaded hole, is connected with a bolt or a screw and is fixed on the piston rod driving plate 1-3, and the lower end of the piston rod 1-6 is provided with a puncture tip 1-9 which can be used for puncture; the upper end of the injection sleeve 1-7 is provided with an annular groove, an upper sealing ring 1-11 is arranged in the annular groove, the outer wall of the upper end of the injection sleeve 1-7 is provided with an external thread and is connected with a sealing nut 1-5, the sealing nut 1-5 limits the upper sealing ring 1-11 in the annular groove of the upper end of the injection sleeve 1-7, the outer wall of the lower end of the injection sleeve 1-7 is provided with a second step surface 1-14, another annular groove for limiting a lower sealing ring 1-12 is also arranged below the second step surface 1-14, and the second step surface 1-14 and the lower sealing ring 1-12 jointly limit the pipetting head 5; in order to ensure that the reagent entering the injector is clean, the upper part of the liquid-moving head 5 is also provided with a filter element 5-1. The piston driving assembly 4 is arranged in the injector assembly 1, wherein a second lead screw motor 4-1 is arranged on a first fixing plate 1-1, a second lead screw nut 4-2 is arranged on a piston rod driving plate 1-3, a lead screw of the second lead screw motor 4-1 penetrates through the first fixing plate 1-1 to be in threaded fit with the second lead screw nut 4-2 on the piston rod driving plate 1-3, and the lower end of the lead screw of the second lead screw motor 4-1 extends into a lead screw hole on the injection sleeve fixing plate 1-4. The piston rods 1-6, the injection sleeves 1-7 and the limiting holes on the injection sleeve fixing plates 1-4 in the injector assembly 1 are in one-to-one correspondence, the number of the limiting holes is equal, 8 limiting holes are arranged on two sides of a screw rod of a second screw rod motor 4-1 in a grouping mode, and the first guide shafts 1-8 are also respectively positioned on two sides of a screw rod of the second screw rod motor 4-1.

In the full-automatic nucleic acid extraction system, the extraction platform assembly 2 is arranged into a plurality of operation areas which are longitudinally divided into a product area 2-1, a liquid transfer area 2-2, a sample area 2-4 and a kit area 2-5, each operation area comprises a plurality of operation positions which are linearly and transversely arranged, the extraction platform assembly 2 is positioned right below the injector assembly 1, and the arrangement of the operation positions corresponds to the limiting holes on the injection sleeve fixing plate 1-4 one by one. In this embodiment, the extraction platform assembly 2 is divided into two groups, each group is 8 rows, each row corresponds to a limiting hole on the injection sleeve fixing plate 1-4, and the product area 2-1 of each group contains 8 product positions, and 8 product cups are respectively placed correspondingly; each group of liquid transfer areas 2-2 comprises two rows of liquid transfer levels, each row is provided with 8 liquid transfer levels, and each liquid transfer level is correspondingly provided with 1 liquid transfer head; the sample area 2-4 of each group contains 8 sample sites, and 8 sample tubes are correspondingly placed in the sample sites. The structure of the reagent kit region 2-5 of each group is shown in FIG. 12, and is divided into an incubation region 2-51, a reagent region 2-52, and a magnetic bead separation region 2-53 in sequence from front to back. In this example, the incubation zones 2-51 comprise two rows of 8 heating cups; the reagent zone 2-52 at least comprises 5 rows of reagent cups, each row comprises 8 reagent cups, and 1 row of magnetic bead mixed liquor, 1 row of lysate, 2 rows of cleaning solution and 1 row of eluent are respectively placed in the reagent cups; the bead isolation zones 2-53 comprise two rows of 8 bead isolation cups. In order to ensure that the amount of the reagent in the experiment is sufficient, a plurality of rows of reagent cups can be arranged in the reagent zone 2-52 for placing the reagent, and the same reagent is placed in the reagent cups in the same row, for example, 8 rows are arranged in the reagent zone 2-52 in the embodiment, wherein the reagent cups in the first 4 rows are all provided with the lysate. In order to prevent reagent pollution, the mouth of each reagent cup is sealed with an aluminum plastic film, and a filter element 5-1 is also arranged in the liquid transferring head.

In the full-automatic nucleic acid extraction system of the present invention, a magnetic separation assembly 8 is further installed at the rear side of the extraction platform assembly 2, and as shown in fig. 7, the magnetic separation assembly 8 of the present invention comprises a motor kit 8-1, a magnet mounting seat 8-2, a magnet mounting groove 8-3, a magnet block 8-4, two second guide shafts 8-5, two second linear bearings 8-6, a translation support plate 8-7, a connection plate 8-8, a rotation shaft support seat 8-9, a rotation shaft 8-10, a first cam 8-11, a second cam 8-12, a fifth fixing plate 8-13, a second guide shaft mounting plate 8-14, and a motor fixing seat 8-15. A fifth fixed plate 8-13 is arranged on the first translation plate 6-5 and the second translation plate 6-6, a rotating shaft supporting seat 8-9 is arranged below the fifth fixed plate 8-13, a left rotating shaft bracket and a right rotating shaft bracket on the rotating shaft supporting seat 8-9 are used for installing a rotating shaft 8-10, and two second linear bearings 8-6 are also arranged on the fifth fixed plate 8-13; the rotating shafts 8-10 are installed on the left rotating shaft support and the right rotating shaft support through bearings, two ends of each rotating shaft 8-10 are fixedly connected with a first cam 8-11 and a second cam 8-12 respectively, protruding columns are arranged on the end faces of the first cam 8-11 and the second cam 8-12, corresponding grooves are formed in the translational supporting plates 8-7, the translational supporting plates 8-7 are installed on the first cams 8-11, and the protruding columns are limited in the grooves; a position avoiding notch with the width corresponding to that of the fifth fixing plate 8-13 is formed in the translation supporting plate 8-7, and when the translation supporting plate 8-7 ascends, the position avoiding notch enables the translation supporting plate 8-7 to avoid being abutted against the fifth fixing plate 8-13; the translational supporting plate 8-7 is fixedly connected with the lower part of the magnet mounting seat 8-2 through a connecting plate 8-8, a magnet mounting groove 8-3 is formed in the upper part of the magnet mounting seat 8-2, and magnet blocks 8-4, the number and the positions of which correspond to the reagent cups in the magnetic bead separation zone in the reagent kit, are arranged in the magnet mounting groove 8-3; the magnet mounting seat 8-2 is fixedly connected with a second guide shaft mounting plate 8-14, two second guide shafts 8-5 are mounted on the second guide shaft mounting plate 8-14, and the two second guide shafts 8-5 are respectively inserted into two second linear bearings 8-6; the motor kit 8-1 comprises a motor, a main driving wheel, a belt and a secondary driving wheel, the motor is arranged on a motor fixing seat 8-15, the motor fixing seat 8-15 is arranged in the middle of a fifth fixing plate 8-13, the main driving wheel is arranged on a motor rotating shaft, the secondary driving wheel is arranged in the middle of a rotating shaft 8-10 and is connected with the main driving wheel through the belt, and the fifth fixing plate 8-13 is provided with a clearance groove hole for the transmission belt to pass through; the lower bottom surface of the fifth fixing plate 8-13 is provided with a third optical coupler 8-16, a third induction sheet 8-17 is arranged on the position, corresponding to the third optical coupler 8-16, of the rotating shaft 8-10, the shape of the third induction sheet 8-17 is set according to the motion trail of the convex column, the third induction sheet 8-17 rotates along with the rotation of the rotating shaft 8-10, and the vertical position information of the magnet mounting seat 8-2 can be obtained by collecting the position information of the third induction sheet 8-17 rotating by the third optical coupler 8-16. In other embodiments, the driving wheel, belt and driven wheel in the motor kit 8-1 can be replaced by a driving gear, chain and driven gear.

The motor drives the rotating shaft 8-10 to rotate, the rotating shaft 8-10 drives the first cam 8-11 and the second cam 8-12 to rotate, the first cam 8-11 drives the translational supporting plate 8-7 to move up and down, the translational supporting plate 8-7 drives the magnet mounting seat 8-2 and the second guide shaft mounting plate 8-14 to move up and down, and in the process of moving up and down of the second guide shaft mounting plate 8-14, the two second guide shafts 8-5 respectively move in the two second linear bearings 8-6 to guide the up and down movement of the magnet mounting seat 8-2. In the rotating process of the rotating shaft 8-10, the third optocoupler 8-16 collects position information of the third induction sheet 8-17 to generate an electric signal, so that the position of the magnet mounting seat 8-2 is judged. In other embodiments, the first cam 8-11 and the second cam 8-12 are provided with protruding columns, the two translational support plates are fixedly connected with the magnet mounting seat 8-2, the two translational support plates respectively correspond to the first cam 8-11 and the second cam 8-12 and are provided with grooves, the protruding columns are respectively limited in the grooves of the translational support plates on the corresponding sides, and the two translational support plates simultaneously drive the magnet mounting seat 8-2 to move up and down. In this embodiment, the magnet blocks 8 to 4 are all in the shape of a bar, and the number and the installation positions of the magnet blocks correspond to those of the magnetic bead separation cup.

In the full-automatic nucleic acid extraction system of the present invention, the magnetic separation assembly 8 has another implementation manner, specifically as shown in fig. 11, and includes a magnet mounting seat 8-2, a magnet mounting groove 8-3, a magnet block 8-4, two second guide shafts 8-5, two second linear bearings 8-6, a fifth fixing plate 8-13, a second guide shaft mounting plate 8-14, a third optical coupler 8-16, a third induction sheet 8-17, a fourth lead screw motor 8-18, a fourth lead screw nut 8-19, and a lead screw connection base plate 8-20. A fifth fixing plate 8-13 is arranged on the first translation plate 6-5 and the second translation plate 6-6, a fourth screw motor 8-18 and two second linear bearings 8-6 are arranged on the fifth fixing plate 8-13, and a third optical coupler 8-16 is further arranged on the lower bottom surface of the fifth fixing plate 8-13; a magnet mounting groove 8-3 is formed above the magnet mounting seat 8-2, magnet blocks 8-4 the number and the positions of which correspond to the reagent cups in the magnetic bead separation area in the reagent kit are arranged in the magnet mounting groove 8-3, the magnet blocks 8-4 are all in a strip shape, and the number and the mounting positions of the magnet blocks correspond to the magnetic bead separation cups; a second guide shaft mounting plate 8-14 and a screw rod connecting bottom plate 8-20 are fixedly connected below the magnet mounting seat 8-2; two second guide shafts 8-5 are mounted on the second guide shaft mounting plates 8-14, and the two second guide shafts 8-5 are respectively inserted into the two second linear bearings 8-6; a fourth screw nut 8-19 is arranged on the screw connecting bottom plate 8-20, and the fourth screw nut 8-19 is in threaded fit with a transmission screw of a fourth screw motor 8-18; a third induction sheet 8-17 is arranged at the position, corresponding to the third optocoupler 8-16, on the lead screw connecting bottom plate 8-20, and a position avoiding notch for the third induction sheet 8-17 to rise and pass through is further arranged at the position, corresponding to the third optocoupler 8-16, on the fifth fixing plate 8-13. The fourth screw motor 8-18 drives the transmission screw to rotate, a fourth screw nut 8-19 in threaded fit with the transmission screw is immediately moved up and down along the transmission screw together with the screw connecting bottom plate 8-20, the magnet mounting seat 8-2 fixedly connected with the screw connecting bottom plate 8-20 and the second guide shaft mounting plate 8-14 are also moved up and down together, and the two second guide shafts 8-5 move in the two linear bearings 8-6 respectively to guide the up and down movement of the magnet mounting seat 8-2. In the process that the screw rod is connected with the third induction sheets 8-17 on the bottom plates 8-20 to move up and down, the third optocouplers 8-16 collect position information of the third induction sheets 8-17 to generate electric signals, and therefore the moving position of the magnet mounting seat 8-2 is judged. The embodiment greatly simplifies the structure of the magnetic separation component in the full-automatic nucleic acid extraction system, further reduces the difficulty of equipment manufacture and reduces the probability of equipment failure.

The syringe assembly of the first embodiment of the full-automatic nucleic acid extraction system of the invention has the following working process:

1. and (5) puncturing the aluminum-plastic film. The extraction platform driving assembly 6 drives the extraction platform assembly 2 to move back and forth, after a specified reagent cup is just opposite to the lower part of the injection sleeve 1-7, the first lead screw motor 3-1 drives the sliding block to move downwards along the first guide rail 3-2 and the second guide rail 3-3 to drive the injector assembly 1 to move downwards integrally, so that the lower end of the injection sleeve 1-7 is close to an encapsulation film of the reagent cup, the second lead screw motor 4-1 drives the piston rod driving plate 1-3 to move downwards, the piston rod driving plate 1-3 drives the piston rod 1-6 to move downwards, so that the puncture tip 1-9 of the piston rod 1-6 is exposed out of the injection sleeve 1-7, and aluminum plastic film puncture is carried out on the reagent cup below.

2. The syringe takes the pipette tip. The extraction platform driving assembly 6 drives the extraction platform assembly 2 to move back and forth, after the designated liquid transfer position is opposite to the lower part of the injector, the first screw motor 3-1 drives the slide block to move downwards along the first guide rail 3-2 and the second guide rail 3-3 to drive the injector assembly 1 to move downwards integrally, the injection sleeve fixing plate 1-4 drives the injection sleeve 1-7 to move downwards, so that the lower end of the injection sleeve 1-7 extends into the liquid transfer head 5 on the liquid transfer position and continues to press the liquid transfer head 5 downwards until the liquid transfer head 5 is in close fit with the liquid transfer head, and the liquid transfer head taking operation is completed. The second step surfaces 1-14 at the lower ends of the injection sleeves 1-7 limit the upper end of the liquid transfer head 5, and the lower sealing rings 1-12 are abutted against the inner wall of the liquid transfer head 5, so that the friction force is generated on the inner wall of the liquid transfer head 5 while the sealing effect is achieved, and the liquid transfer head 5 is prevented from sliding downwards.

3. And (4) sucking liquid by using a syringe. The extraction platform driving assembly 6 drives the extraction platform assembly 2 to move back and forth, after a designated punctured reagent cup is opposite to the lower part of a pipetting head connected with an injector, the first lead screw motor 3-1 drives the sliding block to move downwards along the first guide rail 3-2 and the second guide rail 3-3 to drive the injector assembly 1 to move downwards integrally, so that the pipetting head extends into the reagent cup, the second lead screw motor 4-1 drives the piston rod driving plate 1-3 to move upwards, the piston rod driving plate 1-3 drives the piston rod 1-6 to move upwards, the piston rod 1-6 moves upwards relative to the injection sleeve 1-7, negative pressure exists in the injection sleeve 1-7, and the reagent in the reagent cup is sucked into the injector.

4. And (6) discharging liquid by using the syringe. The extraction platform driving assembly 6 drives the extraction platform assembly 2 to move back and forth, after a designated reagent cup is opposite to the lower part of a pipetting head connected with an injector, the first lead screw motor 3-1 drives the sliding block to move downwards along the first guide rail 3-2 and the second guide rail 3-3 to drive the injector assembly 1 to move downwards integrally, so that the pipetting head extends into the reagent cup, the second lead screw motor 4-1 drives the piston rod driving plate 1-3 to move downwards, the piston rod driving plate 1-3 drives the piston rod 1-6 to move downwards, the piston rod 1-6 moves downwards relative to the injection sleeve 1-7, and the reagent in the reagent cup is discharged into the reagent cup.

5. The syringe removes the pipette tip. The extraction platform driving assembly 6 drives the extraction platform assembly 2 to move back and forth, after the designated pipetting position is just opposite to the lower part of the pipetting head 5, the second screw rod motor 4-1 drives the piston rod driving plate 1-3 to move downwards, the piston rod driving plate 1-3 drives the piston rod 1-6 to move downwards, so that the puncture tip 1-9 of the piston rod 1-6 is abutted against the filter element 5-1 in the pipetting head 5 and is continuously pressed downwards until the pipetting head 5 is separated from the injection sleeve 1-7 and falls into the corresponding pipetting position, and the pipetting head removing operation is completed.

As shown in FIG. 8, the internal mechanical structure of the second embodiment of the fully automatic nucleic acid extracting system of the present invention comprises two sets of syringe assemblies 1, two sets of extracting platform assemblies 2, two sets of piercing driving assemblies 9, two sets of pipetting head removing driving assemblies 10, two sets of liquid sucking and discharging driving assemblies 11, one set of extracting platform driving assemblies 6, and a mounting base 7. The structure of the mounting seat 7 in this embodiment is the same as that in the first embodiment, and the extraction platform driving assembly 6 is different from the first embodiment in that a cable protection chain 6-10 is further included, the cable protection chain 6-10 is mounted on the sliding member 6-2, the electrical conductors of the extraction platform driving assembly 6 are all wrapped in the cable protection chain 6-10, the sliding member 6-2 moves back and forth to drive the electrical conductors connected with the extraction platform driving assembly 6 to move together, and the cable protection chain 6-10 plays a role in protecting and guiding the electrical conductors wrapped inside. The puncture driving assembly 9 in this embodiment is similar to the syringe driving assembly 3 in the first embodiment in structure, and also includes a first lead screw motor 3-1, a first guide rail 3-2, a second guide rail 3-3, and a slider 3-6, the first lead screw motor 3-1 is mounted on a third fixing plate 7-3, the first guide rail 3-2 and the second guide rail 3-3 are mounted on a vertical plate 7-4, the slider 3-6 is in threaded connection (not shown) with a lead screw of the first lead screw motor 3-1 and is in sliding connection with the first guide rail 3-2 and the second guide rail 3-3, two opto-couplers for detecting position information of the slider 3-6 are further mounted on the vertical plate 7-4, an induction sheet is mounted on the slider 3-6, the two opto-couplers respectively correspond to the upper and lower ends of the first guide rail 3-2, the optical coupler generates an electric signal related to the position of the sliding block 3-6 by collecting the position information of the induction sheet on the sliding block 3-6.

As shown in FIG. 9 and FIG. 10, the second embodiment of the fully automatic nucleic acid extracting system of the present invention comprises two sets of syringe assemblies 1 ', each set of syringe assemblies 1 ' comprises a first fixing plate 1-1 ', a second fixing plate 1-2 ', a piston rod driving plate 1-15, an injection sheath driving plate 1-16, a syringe clamping plate 1-17, 8 piston rods 1-6 ', 8 injection sheaths 1-7 ', 3 first guide shafts 1-8 ', and 8 puncture needles 1-18; the second fixing plate 1-2' is fixed on a sliding block 3-6 in the injector driving component 3 and slides up and down along the first guide rail 3-2 and the second guide rail 3-3 along with the sliding block 3-6; the first fixing plate 1-1 'and the syringe clamping plate 1-17 are respectively and fixedly arranged at the upper end and the lower end of the second fixing plate 1-2', and the first guide shaft 1-8 'sequentially and vertically penetrates through the piston rod driving plate 1-15 and the injection sleeve driving plate 1-16 from top to bottom and is fixedly arranged between the first fixing plate 1-1' and the syringe clamping plate 1-17; the injection sleeve 1-7 ' is divided into an empty barrel and a nipple, the cross section of the empty barrel is large and is used for the piston rod 1-6 ' to slide on the inner wall of the empty barrel, the cross section of the nipple is small and is used for installing a liquid transfer head, the injector clamping plate 1-17 is provided with clamping grooves corresponding to the number and the positions of the injectors, the size of each clamping groove is slightly larger than the cross section area of the nipple but smaller than the cross section area of the empty barrel, the upper end of the empty barrel is fixed on the lower bottom surface of the injection sleeve driving plate 1-16, the nipple is limited in the clamping grooves and can slide in the clamping grooves, the upper end of the piston rod 1-6 ' is fixed on the lower bottom surface of the piston rod driving plate 1-; the lower bottom surface of the injector clamping plate 1-17 is provided with a plurality of puncture needles 1-18, and the puncture needles correspond to the clamping grooves one by one. A liquid transfer head taking and releasing drive assembly 10 and a liquid suction and discharge drive assembly 11 are arranged in an injector assembly 1 ', wherein a fifth screw motor 10-1 is arranged on a first fixing plate 1-1 ', a fifth screw nut 10-2 is arranged on an injection sleeve driving plate 1-16, and a screw of the fifth screw motor 10-1 passes through the first fixing plate 1-1 ' and a piston rod driving plate 1-15 and is in threaded fit with the fifth screw nut 10-2; a motor hole for inserting and lifting a sixth lead screw motor 11-1 is formed in the first fixing plate 1-1', a piston rod driving plate 1-15 is provided with a sixth lead screw nut 11-2, the sixth lead screw motor 11-1 penetrates through the motor hole and is fixedly connected with an injection sleeve driving plate 1-16 through a sixth fixing plate 11-3, a lead screw of the sixth lead screw motor 11-1 is in threaded fit with the sixth lead screw nut 11-2, and the lower end of the lead screw is limited on the injection sleeve driving plate 1-16. The piston rods 1-6 ', the injection sleeves 1-7 ', the puncture needles 1-18 and the clamping grooves in each group of injector components 1 ' are in one-to-one correspondence, and the number of the piston rods, the injection sleeves, the puncture needles and the clamping grooves is equal, and in the embodiment, the number of the piston rods, the number of the puncture needles and the clamping grooves is 8; the number of the first guide shafts 1 to 8 is 3 in the present embodiment; the seal between the piston rod 1-6 'and the injection sleeve 1-7' is an oil seal.

The structure of the extraction platform assembly 2 in this embodiment is the same as that of the first embodiment, the extraction platform assembly 2 is also arranged into a plurality of operation areas, and the operation areas are sequentially divided into a product area 2-1, a liquid transfer area 2-2, a sample area 2-4 and a reagent kit area 2-5 from front to back, each operation area comprises a plurality of operation positions which are arranged in a straight line, the arrangement of the operation positions corresponds to the clamping grooves one by one, and the structure of the reagent kit is shown in fig. 12. The extraction platform component 2 is also divided into two groups, each group is 8 rows, each row corresponds to the clamping groove on the syringe clamping plate 1-17, and a filter element or a filter screen is also arranged in the pipetting head.

The working process of the injector assembly of the second embodiment of the full-automatic nucleic acid extraction system is as follows:

1. and (5) puncturing the aluminum-plastic film. The extraction platform driving assembly 6 drives the extraction platform assembly 2 to move back and forth, after the appointed reagent cup is just opposite to the lower part of the puncture needle 1-18, the first lead screw motor 3-1 drives the slide block 3-6 to move downwards along the first guide rail 3-2 and the second guide rail 3-3, so as to drive the injector assembly 1' to move downwards integrally, so that the puncture needle 1-18 reaches the upper part of the puncture reagent cup, and the aluminum plastic film puncture is carried out.

2. The syringe takes the pipette tip. The extraction platform driving assembly 6 drives the extraction platform assembly 2 to move back and forth, after the designated pipetting position is just opposite to the lower part of the injector nipple, the fifth screw motor 10-1 drives the injection sleeve driving plate 1-16 to move down, the injection sleeve is pressed down until the injector nipple is tightly matched with the pipetting head on the pipetting position, and the pipetting head fetching operation is completed. In the process, the sixth screw motor 11-1 moves downwards along with the downwards movement of the injection sleeve driving plate 1-16.

3. And (4) sucking liquid by using a syringe. The extraction platform driving assembly 6 drives the extraction platform assembly 2 to move back and forth, after a designated punctured reagent cup is opposite to the lower part of a pipetting head connected with the injector, the fifth screw motor 10-1 drives the injection sleeve driving plate 1-16 to move down to enable the pipetting head to extend into the reagent cup, the sixth screw motor 11-1 drives the piston rod driving plate 1-15 to move up again, at the moment, the injection sleeve driving plate 1-16 keeps still, the piston rod 1-6 ' moves upwards relative to the injection sleeve 1-7 ', negative pressure exists in the injection sleeve 1-7 ', and the reagent in the reagent cup is sucked into the injector.

4. And (6) discharging liquid by using the syringe. The extraction platform driving assembly 6 drives the extraction platform assembly 2 to move back and forth, after a designated reagent cup is opposite to the lower part of a pipetting head connected with an injector, the fifth screw motor 10-1 drives the injection sleeve driving plate 1-16 to move down, so that the pipetting head extends into the reagent cup, the sixth screw motor 11-1 drives the piston rod driving plate 1-15 to move down, at the moment, the injection sleeve driving plate 1-16 keeps still, the piston rod 1-6 'moves downwards relative to the injection sleeve 1-7', and the reagent in the reagent cup is discharged into the reagent cup.

5. The syringe removes the pipette tip. The extraction platform driving component 6 drives the extraction platform component 2 to move back and forth, after the designated pipetting position is just opposite to the lower part of the injector, the fifth screw motor 10-1 drives the injection sleeve driving plate 1-16 to move up, the injector nipple rises and passes through the clamping groove of the injector clamping plate 1-17, and the pipetting head is limited below the injector clamping plate 1-17 by the clamping groove until the pipetting head is separated from the injector nipple and falls into the corresponding pipetting position, so that the pipetting head removing operation is completed. In the process, the sixth screw motor 11-1 also moves upwards along with the upward movement of the injection sleeve driving plate 1-16.

The full-automatic nucleic acid extraction system of the invention also comprises a control module and a heating rod, wherein the heating rod is used for heating the incubation areas 2-51, the control module can be realized by adopting an integrated circuit, the control module is respectively connected with the extraction platform driving component, the magnetic separation component, the heating rod and injector driving component, the piston driving component or the puncture driving component, the pipetting head taking and releasing driving component and the liquid suction and discharge driving component, the control module is also electrically connected with all the optical couplers in the full-automatic nucleic acid extraction system, and the on/off of each module is controlled by a circuit, and the electric signals fed back by the optical couplers are calculated and analyzed, so that the puncturing, pipetting head taking-off and liquid suction and discharge actions of the injector assembly, the left and right translation of the extraction platform assembly and the up and down movement of the magnetic separation assembly are realized, and the control module also controls the heating process of the incubation area 2-51. The working steps of the full-automatic nucleic acid extraction system can be realized by setting control logic in a control system, and the specific nucleic acid extraction working process is as follows:

1. magnetic beads were prepared. Repeatedly sucking and discharging the magnetic bead mixed solution by using a syringe to uniformly mix the magnetic bead mixed solution; and (4) transferring the magnetic bead mixed solution to a first row of magnetic bead separation cups.

2. The cracking temperature is prepared. The heating rod is activated to bring the temperature of the incubation zone 2-51 to the lysis temperature, typically 56 degrees celsius.

3. And (6) cracking the sample. Pipetting the lysate into the first row of heated cups with a syringe; then sucking the sample in the sample test tube and transferring the sample to the first row of heating cups; repeatedly sucking and discharging the mixed solution by using an injector to uniformly mix the mixed solution; the mixture is incubated for about 10 minutes, waiting for the cleavage reaction to complete.

4. And (4) binding nucleic acid. Transferring the mixed solution after the cracking to a first row of magnetic bead separation cups, and repeatedly sucking and discharging the mixed solution by using a syringe to ensure that the magnetic beads are fully combined with the nucleic acid molecules separated by the cracking to form a magnetic bead-nucleic acid compound; the motor suite 8-1 drives the magnet mounting seat to ascend, so that magnetic beads in the magnetic bead separation cup are adsorbed to the cup wall for magnetic bead separation, supernatant in the magnetic bead separation cup is transferred to the original reagent cup for absorbing lysate, the motor suite 8-1 drives the magnet mounting seat to descend, and the magnetic field outside the reagent cup is removed.

5. And (5) washing the magnetic beads. Transferring the first row of cleaning solution to a first row of magnetic bead separation cups, and repeatedly sucking and discharging the solution for about 2 minutes by using an injector, so that impurities adsorbed on the surfaces of the magnetic beads are gradually dissociated in the cleaning solution in the uniform mixing and cleaning process; the motor suite 8-1 drives the magnet mounting seat to ascend, so that magnetic beads in the magnetic bead separation cup are adsorbed to the cup wall for magnetic bead separation, supernatant in the magnetic bead separation cup is transferred to a reagent cup which originally absorbs cleaning liquid, the motor suite 8-1 drives the magnet mounting seat to descend, and the magnetic field outside the reagent cup is removed.

6. And (5) repeatedly washing the magnetic beads. And (4) transferring the cleaning solution of other rows to the magnetic bead separation cup of the first row, and repeating the magnetic bead cleaning step. This step can be repeated several times according to different experimental requirements or reagent system requirements, and correspondingly, the reagent zones 2-52 are provided with a plurality of rows of cleaning solutions.

7. And removing the ethanol. And (3) standing the magnetic beads subjected to magnetic separation for about 5 minutes to fully volatilize ethanol or other volatile liquid on the surfaces of the magnetic beads. An exhaust fan can be additionally arranged in the full-automatic nucleic acid extraction system to accelerate the volatilization of the volatile liquid on the surface of the magnetic beads.

8. And (4) eluting the nucleic acid. Transferring the eluent into a first row of magnetic bead separation cups, and repeatedly sucking and discharging the solution by using an injector to mix uniformly; pipetting the mixture into a second row of heating cups, and starting the heating rod to bring the temperature of the incubation zone 2-51 to the elution temperature, generally 56 ℃; the mixture was incubated for about 10 minutes, waiting for the elution reaction to complete.

9. And (3) transferring nucleic acid. And (3) pipetting the elution mixed liquid in the heating cups of the second row into the magnetic bead separation cups of the second row, driving the magnet mounting seat to ascend by the motor suite 8-1, enabling the magnetic beads in the magnetic bead separation cups to be adsorbed on the cup wall, carrying out magnetic bead separation, replacing a new pipetting head by the injector, pipetting the eluent into the product cup, obtaining a concentrated sample with high purity by the product cup after the nucleic acid extraction is finished, driving the magnet mounting seat to descend by the motor suite 8-1, and removing the magnetic field outside the reagent cup. In the step, the injector is replaced by a new liquid-transferring head, so that the pollution of the reagent in the step on the nucleic acid purification product is avoided.

In order to minimize the loss of magnetic beads, all the magnetic separation processes in the above steps should last at least 30 seconds, so that the supernatant can be extracted. The mechanical structure of the full-automatic nucleic acid extraction system is not limited to the three embodiments, wherein the injector assembly, the magnetic separation assembly and the extraction platform assembly carrying the kit are all modularized, a new full-automatic nucleic acid extraction system can be freely combined and constructed, and the flux can be increased by expanding according to experimental needs, for example, the number of injectors in the injector assembly is increased, the number of extraction platform assemblies is correspondingly increased, the number of operation positions on the extraction platform assembly is consistent with the number of injectors, the size of the magnet mounting seat is increased, and the number and the placement positions of the magnet blocks in the magnet mounting groove are both corresponding to the magnetic bead separation cups in the kit on the extraction platform assembly.

Claims

1. A full-automatic nucleic acid extraction system comprises a mounting seat, an injector assembly, an extraction platform assembly, a magnetic separation assembly, a kit and a driving assembly, wherein the injector assembly and the extraction platform assembly are both mounted on the mounting seat; the magnetic separation assembly is arranged on the extraction platform assembly and moves along with the extraction platform assembly, and the driving assembly drives the magnetic separation assembly to move up and down so that the magnetic separation assembly performs magnetic bead separation on the solution in the reagent kit; the driving component drives the injector component to move up and down, so that the injector component punctures an encapsulation film on the reagent box, or the injector component takes/takes off a liquid-moving head on the extraction platform component, or the injector component sucks/discharges reagents in the reagent box.

2. The system for extracting nucleic acid according to claim 1, wherein the syringe assembly comprises a syringe composed of a fixing member, a syringe driving member, at least one set of piston rod and a syringe sleeve, the syringe driving member, the piston rod and the syringe sleeve are all mounted on the fixing member, the lower end of the piston rod is provided with a puncture tip, and the syringe driving member comprises a piston driving member and a syringe sleeve driving member; the injection sleeve driving part drives the injection sleeve to move downwards, so that the injection sleeve is worn on the liquid transferring head on the extraction platform assembly; the piston driving piece drives the piston rod to move downwards, so that the puncture tip punctures an encapsulation film on the kit, or the piston rod pushes a liquid transfer head on the injection sleeve until the liquid transfer head falls off; the piston driving piece drives the piston rod to move up and down, so that the injector sucks/discharges the reagent in the reagent box.

3. The full-automatic nucleic acid extraction system according to claim 2, wherein the fixing member comprises a first fixing plate (1-1), a second fixing plate (1-2), a piston rod driving plate (1-3) and an injection sleeve fixing plate (1-4), the first fixing plate (1-1) and the injection sleeve fixing plate (1-4) are respectively and fixedly installed at the upper end and the lower end of the second fixing plate (1-2), a limiting hole corresponding to the injector is formed in the injection sleeve fixing plate (1-4), the injection sleeve (1-7) is fixed in the limiting hole, the upper end of the piston rod (1-6) is fixed on the piston rod driving plate (1-3), and the lower end of the piston rod passes through the injection sleeve fixing plate (1-4) and is inserted into the injection sleeve (1-7); the injection sleeve driving piece drives the second fixing plate (1-2) to move up and down, so that the injection sleeve fixing plate (1-4) drives the injection sleeve (1-7) to move up and down; the piston driving piece drives the piston rod driving plate (1-3) to move up and down, so that the piston rod (1-6) moves up and down.

4. The system for extracting nucleic acid according to claim 1, wherein the syringe assembly comprises a fixing member, and a syringe comprising a puncture needle, a syringe driving member, at least one set of piston rods, and a syringe cover, the puncture needle, the syringe driving member, the pipette head removing driving member, and the pipette liquid driving member being mounted on the fixing member; the puncture driving piece drives the puncture needle to move up and down, so that the puncture needle punctures the packaging film on the kit; the pipetting head taking-off driving part drives the injection sleeve to move up and down, so that the injection sleeve takes off the pipetting head on the extraction platform assembly; the liquid suction and discharge driving part drives the piston rod to move up and down, so that the injector performs suction/discharge actions on the reagent in the reagent box.

5. The full-automatic nucleic acid extraction system according to claim 4, wherein the fixing member comprises a first fixing plate (1-1 '), a second fixing plate (1-2'), a piston rod driving plate (1-15), an injection sleeve driving plate (1-16) and an injector clamping plate (1-17), the first fixing plate (1-1 ') and the injector clamping plate (1-17) are respectively and fixedly arranged at the upper end and the lower end of the second fixing plate (1-2'), a clamping groove corresponding to the injector is arranged on the injector clamping plate (1-17), the upper end of the injection sleeve (1-7 ') is fixed on the injection sleeve driving plate (1-16), the lower part of the injection sleeve (1-7') is limited in the clamping groove and can slide in the clamping groove, the upper end of the piston rod (1-6 ') is fixed on the piston rod driving plate (1-15) and the lower end of the piston rod (1-6') passes through the injection sleeve driving plate The plate (1-16) is inserted into an injection sleeve (1-7'), and the puncture needle (1-18) is arranged on the lower bottom surface of the injector clamping plate (1-17); the pipetting head taking-off driving piece drives the injection sleeve driving plates (1-16) to move up and down, so that the injection sleeves (1-7') move up and down; the liquid suction and discharge driving part drives the piston rod driving plates (1-15) to move up and down, so that the piston rods (1-6') move up and down; the puncture driving piece drives the second fixing plate (1-2') to move up and down, so that the syringe clamping plate (1-17) drives the puncture needle (1-18) to move up and down.

6. The fully automatic nucleic acid extraction system according to claim 1, wherein the magnetic separation assembly comprises a fifth fixing plate, a magnet mounting seat and a magnetic separation driving member, the magnetic separation assembly is mounted on the extraction platform assembly through the fifth fixing plate, the magnetic separation driving member is mounted on the fifth fixing plate, and a plurality of magnet blocks are mounted on the magnet mounting seat; the magnetic separation driving part drives the magnet mounting seat to move up and down, so that the magnet block is close to or far away from the magnetic bead solution in the reagent box to perform magnetic bead separation.

7. The automatic nucleic acid extracting system of claim 6, wherein the magnetic separation driving member comprises a motor assembly (8-1) and a rotating shaft (8-10), at least one cam is installed on the rotating shaft (8-10), the motor assembly (8-1) drives the rotating shaft (8-10) to rotate, the rotating shaft (8-10) drives the cam to rotate, and the cam drives the magnet mounting seat (8-2) to move up and down.

8. The full-automatic nucleic acid extraction system according to claim 6, wherein the magnetic separation driving member comprises a screw motor, the magnet mounting seat is correspondingly provided with a screw nut, and the screw nut is in threaded connection with a transmission screw of the screw motor; the screw motor drives the transmission screw to rotate, so that the screw nut drives the magnet mounting seat to move up and down along the transmission screw.

9. The fully automatic nucleic acid extraction system of claim 1, wherein the extraction platform assembly is provided with a product area (2-1), a pipetting area (2-2), a sample area (2-4) and a kit area (2-5) for carrying a kit, the product area (2-1) is used for placing the final product of nucleic acid extraction, the pipetting area (2-2) is used for placing a pipetting head (5), the sample area (2-4) is used for placing the sample to be tested, the kit is provided with an incubation area (2-51), a reagent area (2-52) and a separation area (2-53), the incubation area (2-51) is used for processing the reaction to be heated, and the reagent area (2-52) is used for placing various reagents required by the test, the magnetic bead separation zone (2-53) is used for processing the operation of magnetic bead separation.

10. The fully automated nucleic acid extraction system of claim 9, further comprising a control module and a heating rod, wherein the heating rod is located below the incubation zone (2-51), the control module is connected to the drive assembly and the heating rod, respectively, and the control module is configured to control the progress of the nucleic acid extraction experiment.