CN115521863B - Nucleic acid analysis equipment - Google Patents
Nucleic acid analysis equipment Download PDFInfo
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- CN115521863B CN115521863B CN202211344337.5A CN202211344337A CN115521863B CN 115521863 B CN115521863 B CN 115521863B CN 202211344337 A CN202211344337 A CN 202211344337A CN 115521863 B CN115521863 B CN 115521863B
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- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 54
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 54
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 54
- 238000004458 analytical method Methods 0.000 title claims abstract description 20
- 239000007788 liquid Substances 0.000 claims description 9
- 239000003153 chemical reaction reagent Substances 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 6
- 239000003292 glue Substances 0.000 claims description 4
- 238000003466 welding Methods 0.000 claims description 2
- 238000006073 displacement reaction Methods 0.000 abstract description 5
- 238000000605 extraction Methods 0.000 description 18
- 230000003287 optical effect Effects 0.000 description 4
- 238000001514 detection method Methods 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 210000003437 trachea Anatomy 0.000 description 2
- 208000035473 Communicable disease Diseases 0.000 description 1
- 208000026350 Inborn Genetic disease Diseases 0.000 description 1
- 102000011931 Nucleoproteins Human genes 0.000 description 1
- 108010061100 Nucleoproteins Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 208000016361 genetic disease Diseases 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
- C12M33/07—Dosage or metering devices therefore
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a nucleic acid analysis apparatus. A workbench is arranged on the frame, and a cross beam, a longitudinal beam and a pulley are arranged above the workbench; the pulley is connected with the quantitative nucleic acid pipettor through the lifting cylinder; the quantitative nucleic acid pipette comprises a pipetting assembly and a sleeve assembly; the pipetting assembly comprises a movable plate, a motor is fixed on the upper side surface of the movable plate, and a screw is fixed at the output end of the motor; the connecting disc is in threaded connection with the screw rod; a plurality of sliding rods are fixed on the connecting disc, and the sliding rods downwards slide through the moving plate; the lower side of the moving plate is fixed with a sucker; the sleeve assembly comprises a sliding seat, and a supporting round table is slidably arranged on the sliding seat; a pipetting cylinder is arranged in the supporting round table mounting seat hole; a piston rod is arranged in the pipetting cylinder in a matched manner. According to the invention, the relative displacement of the pipetting cylinder and the piston rod is controlled, so that the purpose of accurately and quantitatively sucking samples is achieved; and the combination of the pipetting component, the sleeve component and the analyzer realizes automatic control and improves the operation precision and the operation efficiency.
Description
Technical Field
The invention relates to the technical field of nucleic acid detection and analysis equipment, in particular to nucleic acid analysis equipment.
Background
Nucleic acid is a biological macromolecular compound polymerized from many nucleotides, and is one of the most basic substances for life. Nucleic acids are widely found in all animal and plant cells and microorganisms, and nucleic acids in organisms are often combined with proteins to form nucleoproteins. In the medical field, nucleic acid analysis plays an important role in diagnosing infectious diseases or genetic diseases at the gene level, and is now applied not only to the medical field but also to various fields such as agriculture and food fields. In general, nucleic acid analysis is performed by purifying nucleic acid derived from a sample, amplifying the purified nucleic acid, and detecting the amplified nucleic acid. The use of an extraction device to place a sample into a kit is required during nucleic acid analysis.
Chinese patent discloses a nucleic acid extraction device (CN 217459379U) for nucleic acid extraction appearance, including the extraction tube, the bottom intercommunication of extraction tube has the pipette, the extraction tube is close to top one side and is fixed with the handle, wherein, the handle top rotates and is connected with presses the handle, press being connected with the gasbag between handle and the handle, the internally mounted of gasbag has the spring, the gasbag is close to below one side and is linked together with the extraction tube through the trachea. This extraction element passes through the handle and drives the extraction tube and remove, later presses and presses the handle, presses the handle and drives the gasbag compression, and the extraction tube is inside with the interior air discharge of gasbag, makes the piston move down, later drives in the liquid suction head of extraction tube inserts the nucleic acid solution through the handle, loosens and presses the handle, and the spring promotes the gasbag and swells, makes the gasbag pass through the trachea and drives the piston and upwards remove, inhales the inside of extraction tube with the nucleic acid solution, through handle and the gasbag suction structure that sets up, can make the extraction tube keep stable, is convenient for draw the nucleic acid solution.
The defects of the prior art are as follows: the air bag is pushed to bulge by the spring, and the air bag sucks out the gas in the air pipe, so that the piston moves upwards to suck the nucleic acid solution into the extraction pipe; the sucking process is not easy to control, the extraction amount is not accurate, and the method is not suitable for quantitative analysis of nucleic acid; the spring always provides the elasticity of the stretching air bag, the deformation of the air bag is large, the initial state of the extraction pipe is not easy to control, and the extraction is not easy to control and accurate; the manual extraction mode is adopted to extract the nucleic acid, and the spring control mode is difficult to combine with automatic equipment, so that the operation efficiency is low.
Disclosure of Invention
In view of the above-described technical shortcomings, an object of the present invention is to provide a nucleic acid analysis apparatus. The nucleic acid analysis equipment realizes the connection of a suction tube and the analyzer by improving the quantitative nucleic acid pipette to be a detachable pipetting component and a sleeve component, and completes the automatic control of the equipment.
The invention adopts the following technical scheme: the nucleic acid analysis equipment comprises a rack, wherein a workbench is arranged on the rack, and a reagent box is placed in a clamping groove on the workbench; a pair of cross beams are arranged above the workbench, a longitudinal beam is slidably arranged between the two cross beams, and a first pulley is slidably arranged on the longitudinal beam; a first lifting cylinder is fixed under the first pulley, and the lower end of the first lifting cylinder is connected with a quantitative nucleic acid pipette;
the quantitative nucleic acid pipette comprises a pipetting assembly and a sleeve assembly;
the pipetting assembly comprises a horizontal moving plate, a motor is fixed on the upper side surface of the moving plate, and a screw rod perpendicular to the moving plate is fixed at the output end of the motor; a connecting disc is arranged above the motor and is in threaded connection with the screw rod; a plurality of sliding rods are fixed on the connecting disc, and the sliding rods downwards slide through the moving plate; a sucker is fixed on the lower side of the moving plate;
the sleeve assembly comprises a sliding seat, and a supporting round table is slidably arranged on the sliding seat; the supporting round table is provided with a mounting seat hole which penetrates through the supporting round table up and down; the upper end of the mounting seat hole is provided with a liquid transferring cylinder, the lower end of the mounting seat hole is provided with a flexible rubber sleeve, and the flexible rubber sleeve is sleeved at the lower end of the liquid transferring cylinder; a piston rod is arranged in the pipetting cylinder in a matched manner; the sucking disc is in suction connection with the upper end of the piston rod, and the lower end of the sliding rod is propped against the upper end of the pipetting cylinder.
Preferably: the upper surface welding of the one end that the movable plate kept away from the motor is fixed with the flange pole, and the flange pole is perpendicular with the movable plate, and the flange pole upper end has the ring flange.
Preferably: three sliding rods are arranged on the connecting disc and are uniformly distributed around the screw rod at the output end of the motor; the first limiting snap ring used for limiting the relative position of the screw rod and the connecting disc is arranged on the screw rod, and the second limiting snap ring used for limiting the relative position of the slide rod and the movable plate is arranged on the slide rod.
Preferably: the first limiting snap ring and the second limiting snap ring are shaft retainer rings, circumferential positioning grooves are formed in the screw rod and the sliding rod, and the first limiting snap ring and the second limiting snap ring are arranged in the corresponding positioning grooves of the screw rod and the sliding rod.
Preferably: the sliding sleeve is arranged in the through hole of the moving plate in a sliding manner, and the sliding rod is slidably arranged in the corresponding sliding sleeve.
Preferably: the sliding seat is provided with a T-shaped sliding groove, and the bottom of the T-shaped sliding groove is provided with an avoiding sinking groove which is in the same direction as the T-shaped sliding groove; the lower end of the supporting round table is provided with a step matched with the T-shaped chute.
Preferably: the mounting seat hole of the supporting round table sequentially comprises an inner spigot, a conical section and a straight cylindrical section from top to bottom; the lower end of the liquid transferring cylinder is matched with the inner spigot and the conical section, the upper end of the liquid transferring cylinder is provided with an annular table, and the annular table is propped against the lower end of the sliding rod.
Preferably: the soft rubber sleeve is arranged in the straight cylindrical section of the mounting seat hole, and the lower end of the soft rubber sleeve extends out of the mounting seat hole; the upper end of the soft rubber sleeve is provided with a rim protruding towards the periphery; the clamping groove is formed in the inner wall of the straight cylindrical section, and the edge of the soft rubber sleeve is clamped in the clamping groove.
Preferably: an annular groove is formed in the middle of the piston rod, a friction ring is arranged in the annular groove of the piston rod, and the friction ring is in interference fit with the inner wall of the pipetting cylinder; the upper end of the piston rod is provided with a disc matched with the sucker.
The invention has the beneficial effects that: the miniature motor is adopted to drive the screw rod to rotate, then the connecting disc and the sliding rod push the liquid-moving cylinder downwards, the liquid-moving cylinder and the piston rod relatively move so as to absorb samples, the displacement of the sliding rod can be controlled through the limiting snap ring on the screw rod in the moving process, and the relative displacement of the liquid-moving cylinder and the piston rod is controlled so as to achieve the aim of accurately and quantitatively absorbing the samples; the sucking disc of the pipetting assembly is sucked with the piston rod of the sleeve assembly, and then is combined with the analyzer, so that automatic control is realized, and the operation precision and the operation efficiency are improved; the friction ring is added on the piston rod, so that the friction force between the piston rod and the pipetting cylinder is increased, and the stability of the relative position of the piston rod and the pipetting cylinder is maintained; the sleeve assembly is fixed on the supporting round table and sealed through the soft rubber sleeve, so that pollution is avoided, and when the sleeve assembly is combined with the analyzer, the sleeve assembly moves upwards to automatically fall off the soft rubber sleeve; the support round platform is slidably mounted on the sliding seat, so that the support round platform is convenient to detach and mount, can be recycled, and reduces cost.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a front view of a nucleic acid analyzing apparatus of the present invention.
FIG. 2 is a perspective view (first view) of a quantitative nucleic acid pipette portion of the present invention.
FIG. 3 is a perspective view of a quantitative nucleic acid pipette portion of the present invention (second view, the slide is omitted).
FIG. 4 is a partial top view of a quantitative nucleic acid pipette of the present invention.
Fig. 5 is a view in the direction A-A of fig. 4.
FIG. 6 is a diagram showing the initial state of a quantitative nucleic acid pipette according to the present invention.
Fig. 7 is a schematic view of the pipette cartridge of fig. 6 disengaged from the supporting circular table.
Fig. 8 is a schematic view of the pipette of fig. 6 in relative motion with the piston rod.
Reference numerals illustrate: 1. a moving plate; 2. a motor; 3. a screw; 4. a connecting disc; 5. a slide bar; 6. a suction cup; 7. a slide; 701. t-shaped sliding groove; 702. avoiding the sinking groove; 8. supporting the round table; 801. a mounting seat hole; 8011. an inner spigot; 8012. a conical section; 8013. a straight cylindrical section; 802. a step; 803. a clamping groove; 9. a pipetting cylinder; 901. an annular table; 10. a soft rubber sleeve; 11. a piston rod; 1101. a disc; 12. a flange rod; 1201. a flange plate; 13. a first limit snap ring; 14. the second limiting snap ring; 15. a sliding sleeve; 16. a friction ring; 17. a frame; 18. a work table; 19. a kit; 20. a cross beam; 21. a longitudinal beam; 22. a first sled; 23. a first lifting cylinder; 24. a second sled; 25. a second lifting cylinder; 26. an optical detector; 27. quantitative nucleic acid pipettor.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1, a nucleic acid analyzer is provided with a table 18 on a frame 17, a reagent kit 19 is placed in a clamping groove on the table 18, and an air pump system matched with the reagent kit 19 is installed under the table 18. A pair of cross beams 20 are arranged above the workbench 18, two longitudinal beams 21 are slidably arranged between the two cross beams 20, and electric vehicles for controlling the longitudinal beams 21 to move along the cross beams 20 are arranged at the end parts of the longitudinal beams 21. A first pulley 22 is slidably arranged on one of the longitudinal beams 21, a first lifting cylinder 23 is fixed below the first pulley 22, and a quantitative nucleic acid pipette 27 is connected to the lower end of the first lifting cylinder 23. A second pulley 24 is slidably arranged on the second longitudinal beam 21, a second lifting cylinder 25 is fixed below the second pulley 24, and an optical detector 26 is connected to the lower end of the second lifting cylinder 25. The quantitative nucleic acid pipettor 27 and the optical detector 26 can move in the three-dimensional space through the longitudinal beam 21, the transverse beam 20 and the lifting cylinder, so that samples can be conveniently extracted and data detection can be conveniently carried out.
Referring to FIGS. 2 to 6, the quantitative nucleic acid pipettor 27 includes a pipetting assembly and a cannula assembly;
the pipetting assembly in this embodiment comprises a movable plate 1, a flange rod 12 is welded and fixed on the upper surface of one end of the movable plate 1, the flange rod 12 is perpendicular to the movable plate 1, a flange 1201 is arranged at the upper end of the flange rod 12, and the flange 1201 is connected with the lower end of a first lifting cylinder 23 through bolts. The upper surface of the other end of the movable plate 1 is fixed with a miniature motor 2. The output end of the motor 2 is vertically upwards, and a screw 3 vertical to the movable plate 1 is fixed at the output end of the motor 2. The screw 3 is provided with a circumferential positioning groove, the first limiting snap ring 13 adopts a shaft retainer ring, and the first limiting snap ring 13 is arranged in the positioning groove on the screw 3. The first limiting snap ring 13 is located below the connecting disc 4, and the first limiting snap ring 13 is used for limiting the relative position of the screw 3 and the connecting disc 4.
The center of the connecting disc 4 is provided with a threaded hole, and the screw 3 is penetrated in the threaded hole of the connecting disc 4 through threaded connection. Three slide bars 5 are fixed on the lower surface of the connecting disc 4, and the three slide bars 5 are uniformly distributed around the screw 3 at the output end of the motor 2. The movable plate 1 is provided with a through hole matched with the slide rod 5, a sliding sleeve 15 is fixedly arranged in the through hole of the movable plate 1, and the slide rod 5 is slidably arranged in the corresponding sliding sleeve 15. The slide bar 5 is provided with a circumferential positioning groove, the second limiting snap ring 14 is a shaft retainer ring, and the second limiting snap ring 14 is arranged in the positioning groove of the slide bar 5. The second limiting snap ring 14 is located below the moving plate 1, and the second limiting snap ring 14 is used for limiting the relative position of the sliding rod 5 and the moving plate 1. In the embodiment, the micro motor is adopted to drive the screw rod to rotate, then the connecting disc and the sliding rod push the liquid-moving barrel downwards, the liquid-moving barrel and the piston rod move relatively so as to absorb samples, the displacement of the sliding rod can be controlled through the limiting clamping ring on the screw rod in the moving process, and then the relative displacement of the liquid-moving barrel and the piston rod is controlled, so that the aim of accurately and quantitatively absorbing the samples is fulfilled.
Referring to fig. 1 in combination with fig. 5 to 8, the sleeve assembly comprises a slide 7, the slide 7 being fixed to a table 18. The slide seat 7 is provided with a T-shaped chute 701, and the bottom of the T-shaped chute 701 is provided with an avoiding sinking groove 702 which is in the same direction as the T-shaped chute 701. The lower end of the supporting round table 8 is provided with a step 802 matched with the T-shaped sliding groove 701, the supporting round table 8 can slide along the T-shaped sliding groove 701 of the sliding seat 7, and the T-shaped sliding groove can limit the supporting round table 8 to slide out of the sliding seat 7. The supporting round table 8 slides in from one end of the sliding seat 7 and slides out from the other end.
The supporting round table 8 is provided with a mounting seat hole 801 which vertically penetrates through the supporting round table 8, and the mounting seat hole 801 sequentially comprises an inner spigot 8011, a conical section 8012 and a straight cylindrical section 8013 from top to bottom. The lower end of the pipetting cylinder 9 is mounted in cooperation with the inner spigot 8011 and the conical section 8012. The soft rubber sleeve 10 is sleeved at the lower end of the pipetting cylinder 9. The flexible rubber sleeve 10 is of a conical structure with an open upper end, and the upper end of the flexible rubber sleeve 10 is provided with a rim 1001 protruding towards the periphery. The inner wall of the straight barrel section 8013 is provided with a clamping groove 803. When the device is used, the liquid transferring cylinder 9 and the flexible rubber sleeve 10 are downwards inserted into the mounting seat hole 801 until the edge 1001 of the flexible rubber sleeve 10 is clamped in the clamping groove 803, and the lower end of the liquid transferring cylinder 9 is matched with the inner spigot 8011 and the conical section 8012 in place. In the embodiment, the sleeve assembly is fixed on the supporting circular table and is sealed through the soft rubber sleeve, so that pollution is avoided, and when the sleeve assembly is combined with the analyzer, the sleeve assembly moves upwards to automatically fall off the soft rubber sleeve. The lower end of the flexible glue sleeve 10 extends out of the mounting seat hole 801, and the avoidance sinking groove 702 of the sliding seat 7 can prevent interference with the flexible glue sleeve 10. After the pipetting cylinder 9 on the support round table 8 is used, the flexible rubber sleeve 10 can be removed from the lower part of the support round table 8. In the embodiment, the supporting round table is slidably mounted on the sliding seat, so that the supporting round table is convenient to detach and mount, can be recycled, and reduces cost.
The upper end of the pipetting cylinder 9 has an annular table 901. An annular groove is formed in the middle of the piston rod 11, a friction ring 16 is arranged in the annular groove of the piston rod 11, and the friction ring 16 is in interference fit with the inner wall of the pipetting cylinder 9. The friction ring is added on the piston rod, so that the friction force between the piston rod and the pipetting cylinder can be increased, and the stability of the relative position of the piston rod and the pipetting cylinder is maintained. The upper end of the piston rod 11 has a disc 1101. The sucker 6 is fixed on the lower side of the movable plate 1, and when in use, the sucker 6 is opposite to and attracted with the disc 1101 at the upper end of the piston rod 11; at this time, the annular table 901 at the upper end of the pipetting cylinder 9 is opposed to the lower end of the slide bar 5, and a certain gap is left between the annular table 901 and the slide bar 5, thereby avoiding interference between the suction cup 6 and the disk 1101. In the embodiment, the sucker of the pipetting assembly is sucked with the piston rod of the sleeve assembly, so that the pipetting assembly is combined with the analyzer, automatic control is realized, and the operation precision and the operation efficiency are improved.
In the use process, the water-soluble fiber is prepared,
providing a sleeve assembly: the pipetting cylinder 9 is put into the mounting seat hole 801 of the supporting round table 8 in advance, the flexible rubber sleeve 10 with sealing function at the lower end of the pipetting cylinder 9 is clamped in the clamping groove 803, the supporting round table 8 sequentially slides into the sliding seat 7, and the pipetting cylinder 9 is in a standby state;
the reagent box 19 is matched and placed in a clamping groove on the workbench 18;
the pipetting component of the quantitative nucleic acid pipettor 27 is controlled to move above the sleeve component, the first lifting cylinder 23 descends, and the sucker 6 is opposite to and sucked with the disc 1101 at the upper end of the piston rod 11; then the first lifting cylinder 23 ascends to drive the piston rod 11 and the pipetting cylinder 9 to move upwards, and the pipetting cylinder 9 is separated from the flexible glue sleeve 10;
controlling a pipetting cylinder 9 of the quantitative nucleic acid pipettor 27 to a sample, driving a screw 3 to rotate by a motor 2, and then pushing the pipetting cylinder 9 downwards by a connecting disc 4 and a sliding rod 5, and enabling the pipetting cylinder 9 to move relative to a piston rod 11 so as to suck the sample;
controlling a pipetting cylinder 9 of a quantitative nucleic acid pipettor 27 to reach a reagent box 19, driving a screw rod 3 to reversely rotate by a motor 2, and resetting a connecting disc 4 and a sliding rod 5; then, the lower end step of the pipetting cylinder 9 is propped against the periphery of a reaction hole in the kit 19, the first lifting cylinder 23 descends to push the piston rod 11 to move downwards relative to the pipetting cylinder 9, a sample is extruded, and then the pipetting cylinder 9 is removed;
the air pump system works to control the reaction of the sample in the kit 19, and finally the detection is carried out by the optical detector 26, and information is transmitted to the upper computer.
The preferred embodiments of the invention disclosed above are intended only to assist in the explanation of the invention. The preferred embodiments are not exhaustive or to limit the invention to the precise form disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best understand and utilize the invention. The invention is limited only by the claims and the full scope and equivalents thereof.
Claims (9)
1. The nucleic acid analysis equipment comprises a rack (17), wherein a workbench (18) is arranged on the rack (17), and a reagent box (19) is placed in a clamping groove on the workbench (18); the method is characterized in that: a pair of cross beams (20) are arranged above the workbench (18), longitudinal beams (21) are slidably arranged between the two cross beams (20), and a first pulley (22) is slidably arranged on the longitudinal beams (21); a first lifting cylinder (23) is fixed below the first pulley (22), and the lower end of the first lifting cylinder (23) is connected with a quantitative nucleic acid pipette (27);
the quantitative nucleic acid pipette (27) comprises a pipetting assembly and a cannula assembly;
the pipetting assembly comprises a horizontal moving plate (1), a motor (2) is fixed on the upper side surface of the moving plate (1), and a screw rod (3) perpendicular to the moving plate (1) is fixed at the output end of the motor (2); a connecting disc (4) is arranged above the motor (2), and the connecting disc (4) is in threaded connection with the screw rod (3); a plurality of sliding rods (5) are fixed on the connecting disc (4), and the sliding rods (5) downwards slide through the moving plate (1); a sucker (6) is fixed on the lower side of the moving plate (1);
the sleeve assembly comprises a sliding seat (7), and a supporting round table (8) is slidably arranged on the sliding seat (7); a mounting seat hole (801) which vertically penetrates through the supporting round table (8) is formed in the supporting round table (8); a pipetting cylinder (9) is arranged at the upper end of the mounting seat hole (801), a flexible rubber sleeve (10) is arranged at the lower end of the mounting seat hole (801), and the flexible rubber sleeve (10) is sleeved at the lower end of the pipetting cylinder (9); a piston rod (11) is arranged in the pipetting cylinder (9) in a matched manner; the sucking disc (6) is in suction connection with the upper end of the piston rod (11), and the lower end of the sliding rod (5) is propped against the upper end of the pipetting cylinder (9).
2. The nucleic acid analysis apparatus according to claim 1, wherein: the upper surface welding of the one end that removes board (1) and keep away from motor (2) is fixed with flange pole (12), and flange pole (12) are perpendicular with removal board (1), and flange dish (1201) are had to flange pole (12) upper end.
3. The nucleic acid analysis apparatus according to claim 1, wherein: three sliding rods (5) are arranged on the connecting disc (4), and the three sliding rods (5) are uniformly distributed around a screw rod (3) at the output end of the motor (2); the screw rod (3) is provided with a first limiting snap ring (13) for limiting the relative position of the screw rod (3) and the connecting disc (4), and the slide rod (5) is provided with a second limiting snap ring (14) for limiting the relative position of the slide rod (5) and the movable plate (1).
4. A nucleic acid analysis apparatus according to claim 3, wherein: the first limiting clamp ring (13) and the second limiting clamp ring (14) are shaft retainer rings, circumferential positioning grooves are formed in the screw rod (3) and the sliding rod (5), and the first limiting clamp ring (13) and the second limiting clamp ring (14) are arranged in the corresponding positioning grooves of the screw rod (3) and the sliding rod (5).
5. The nucleic acid analysis apparatus according to claim 1, wherein: the sliding sleeve (15) is fixedly installed in the through hole of the moving plate (1), and the sliding rod (5) is slidably installed in the corresponding sliding sleeve (15).
6. The nucleic acid analysis apparatus according to claim 1, wherein: the slide seat (7) is provided with a T-shaped chute (701), and the bottom of the T-shaped chute (701) is provided with an avoiding sinking groove (702) which is in the same direction as the T-shaped chute (701); the lower end of the supporting round table (8) is provided with a step (802) matched with the T-shaped chute (701).
7. The nucleic acid analysis apparatus according to claim 1, wherein: the mounting seat hole (801) of the supporting round table (8) sequentially comprises an inner spigot (8011), a conical section (8012) and a straight cylindrical section (8013) from top to bottom; the lower end of the liquid transferring tube (9) is matched with the inner spigot (8011) and the conical section (8012), the upper end of the liquid transferring tube (9) is provided with an annular table (901), and the annular table (901) is propped against the lower end of the sliding rod (5).
8. The nucleic acid analysis apparatus according to claim 7, wherein: the soft rubber sleeve (10) is arranged in the straight cylindrical section (8013) of the mounting seat hole (801), and the lower end of the soft rubber sleeve (10) extends out of the mounting seat hole (801); the soft rubber sleeve (10) is of a conical structure with an open upper end, and the upper end of the soft rubber sleeve (10) is provided with a rim (1001) protruding towards the periphery; the inner wall of the straight cylindrical section (8013) is provided with a clamping groove (803), and the edge (1001) of the flexible glue sleeve (10) is clamped in the clamping groove (803).
9. The nucleic acid analysis apparatus according to claim 1, wherein: an annular groove is formed in the middle of the piston rod (11), a friction ring (16) is arranged in the annular groove of the piston rod (11), and the friction ring (16) is in interference fit with the inner wall of the pipetting cylinder (9); the upper end of the piston rod (11) is provided with a disc (1101) matched with the sucker (6).
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| CN202211344337.5A CN115521863B (en) | 2022-10-31 | 2022-10-31 | Nucleic acid analysis equipment |
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| CN202211344337.5A CN115521863B (en) | 2022-10-31 | 2022-10-31 | Nucleic acid analysis equipment |
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| CN115521863B true CN115521863B (en) | 2023-12-05 |
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| CN103586095A (en) * | 2013-11-26 | 2014-02-19 | 东南大学 | Multichannel pipettor |
| CN103691498A (en) * | 2014-01-06 | 2014-04-02 | 广州市刑事科学技术研究所 | Liquid-moving machine and method of moving liquid by same |
| CN110628569A (en) * | 2019-10-16 | 2019-12-31 | 宁波美康盛德生物科技有限公司 | Full-automatic nucleic acid extraction system |
| CN111514952A (en) * | 2020-05-14 | 2020-08-11 | 广东体必康生物科技有限公司 | Pipetting device and method capable of self-checking air tightness and accurately extracting |
| CN214681808U (en) * | 2020-11-27 | 2021-11-12 | 泰兴市求精仪器厂 | Integrated quantitative micro blood sampling pipette |
| CN114836309A (en) * | 2022-04-15 | 2022-08-02 | 上海仁度生物科技股份有限公司 | Full-automatic nucleic acid detection and analysis equipment and system |
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|---|---|---|---|---|
| US6793891B2 (en) * | 2002-04-08 | 2004-09-21 | Felxi Yiu | Pipettor and externally sealed pipette tip |
| CN110913989A (en) * | 2017-07-14 | 2020-03-24 | 迈恩医疗解决方案有限公司 | Automatic pipetting device for transferring samples and/or reagents and method for transferring liquid samples and/or reagents |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103586095A (en) * | 2013-11-26 | 2014-02-19 | 东南大学 | Multichannel pipettor |
| CN103691498A (en) * | 2014-01-06 | 2014-04-02 | 广州市刑事科学技术研究所 | Liquid-moving machine and method of moving liquid by same |
| CN110628569A (en) * | 2019-10-16 | 2019-12-31 | 宁波美康盛德生物科技有限公司 | Full-automatic nucleic acid extraction system |
| CN111514952A (en) * | 2020-05-14 | 2020-08-11 | 广东体必康生物科技有限公司 | Pipetting device and method capable of self-checking air tightness and accurately extracting |
| CN214681808U (en) * | 2020-11-27 | 2021-11-12 | 泰兴市求精仪器厂 | Integrated quantitative micro blood sampling pipette |
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