CN109959782B - Pretreatment device and method for chicken extracting solution - Google Patents
Pretreatment device and method for chicken extracting solution Download PDFInfo
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- CN109959782B CN109959782B CN201910218476.5A CN201910218476A CN109959782B CN 109959782 B CN109959782 B CN 109959782B CN 201910218476 A CN201910218476 A CN 201910218476A CN 109959782 B CN109959782 B CN 109959782B
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- 241000287828 Gallus gallus Species 0.000 title claims abstract description 24
- 238000000034 method Methods 0.000 title description 7
- 239000007788 liquid Substances 0.000 claims abstract description 534
- 238000007885 magnetic separation Methods 0.000 claims abstract description 43
- 239000011324 bead Substances 0.000 claims abstract description 20
- 238000002203 pretreatment Methods 0.000 claims abstract description 7
- 230000003287 optical effect Effects 0.000 claims description 73
- 239000000243 solution Substances 0.000 claims description 66
- 239000003153 chemical reaction reagent Substances 0.000 claims description 40
- 238000001514 detection method Methods 0.000 claims description 23
- 238000002347 injection Methods 0.000 claims description 20
- 239000007924 injection Substances 0.000 claims description 20
- 238000007599 discharging Methods 0.000 claims description 16
- 239000002096 quantum dot Substances 0.000 claims description 9
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 7
- 229910052802 copper Inorganic materials 0.000 claims description 7
- 239000010949 copper Substances 0.000 claims description 7
- 230000001174 ascending effect Effects 0.000 claims description 3
- 238000012840 feeding operation Methods 0.000 claims description 3
- 230000000149 penetrating effect Effects 0.000 claims description 3
- 230000000630 rising effect Effects 0.000 claims 1
- 238000001179 sorption measurement Methods 0.000 abstract description 2
- 239000000523 sample Substances 0.000 description 80
- 238000002965 ELISA Methods 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 239000012472 biological sample Substances 0.000 description 2
- SPFYMRJSYKOXGV-UHFFFAOYSA-N Baytril Chemical compound C1CN(CC)CCN1C(C(=C1)F)=CC2=C1C(=O)C(C(O)=O)=CN2C1CC1 SPFYMRJSYKOXGV-UHFFFAOYSA-N 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 229960000740 enrofloxacin Drugs 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 239000006249 magnetic particle Substances 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
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Abstract
The invention discloses a pretreatment device and a pretreatment method for a chicken extracting solution. The liquid transfer gun comprises a rack, a liquid transfer gun module, a liquid feeding module, a sample feeding module, a liquid withdrawing module, a first liquid suction and discharge module, a second liquid suction and discharge module, a collecting tank and a magnetic separation module, wherein the liquid transfer gun module is arranged in the middle of the rack, the liquid transfer gun module is arranged around the liquid transfer gun module and positioned at the bottom of the rack, the liquid feeding module, the sample feeding module and the collecting tank are respectively arranged on the rack through the rack, the liquid feeding module and the sample feeding module are oppositely arranged, the liquid feeding module and the collecting tank are oppositely arranged, and the magnetic separation module is arranged on one side, close to the liquid feeding module, of the sample feeding; the liquid feeding module is arranged above the upper liquid module and positioned at the top of the rack, the liquid feeding module is arranged above the sample feeding module and positioned at the top of the rack, and the liquid feeding module is arranged above the sample feeding module and positioned at the top of the rack. The invention can improve the mixing speed, the magnetic bead adsorption efficiency and the automation efficiency of the sample to be detected.
Description
Technical Field
The invention belongs to the field of pretreatment of biological sample detection, and particularly relates to a pretreatment device and method for a chicken extracting solution.
Background
In the method for detecting the antibiotic content in the chicken sample (GB/T-21312-2007), according to a national standard pretreatment method, the chicken tissue is smashed and subjected to ultrasonic centrifugation to obtain the chicken extract. And performing high performance liquid chromatography/mass spectrometry detection on the chicken extracting solution. This method of sample presentation detection is time-consuming and expensive, and enzyme-linked immunosorbent assay (ELISA) -based kits are popular in the market. However, ELISA requires a large amount of reagents and is complex to operate, and therefore, a set of automatic equipment is developed for the pretreatment method of the chicken extracting solution, and the problem can be effectively solved.
Disclosure of Invention
In order to solve the problems in the background art, the invention provides a pretreatment device and a pretreatment method for chicken extract, which are mainly used in a pretreatment stage of enrofloxacin antibiotic detection, and a biological sample after treatment can be sent to detection.
The technical scheme adopted by the invention is as follows:
pretreatment device for chicken extracting solution
The liquid transfer gun comprises a rack, a liquid transfer gun module, a liquid feeding module, a sample feeding module, a liquid withdrawing module, a first liquid sucking and discharging module, a second liquid sucking and discharging module, a collecting tank and a magnetic separation module, wherein the liquid transfer gun module is arranged in the middle of the rack; the liquid feeding module is arranged above the upper liquid module and positioned at the top of the rack, the liquid feeding module is arranged above the sample feeding module and positioned at the top of the rack, the liquid feeding module is arranged at the top of the rack, and the liquid discharging module is arranged at the top of the rack.
The liquid-transfering gun module comprises a liquid-transfering gun, a liquid-transfering gun seat, a liquid-transfering gun tray, a liquid-transfering gun module bottom plate, a motor connecting plate, an angular contact ball bearing seat, a fourth rotating motor, a rotating shaft, a photoelectric sensor and a photoelectric sensor detection plate; a bottom plate of the liquid-transferring gun module is fixed at the center of the bottom of the frame, a motor connecting plate is fixed above the bottom plate of the liquid-transferring gun module through two supporting plates, a fourth rotating motor is installed on the lower surface of the motor connecting plate, and an output shaft of the fourth rotating motor penetrates out of the motor connecting plate upwards to be connected with the coupler; an angular contact ball bearing seat is fixed above the upper surface of the motor connecting plate through two copper columns, a rotating shaft is mounted in the middle of the angular contact ball bearing seat through an angular contact ball bearing, a liquid-transfering gun plate is mounted at the upper end of the rotating shaft, the lower end of the rotating shaft is connected with an output shaft of a fourth rotating motor through a coupler, and a photoelectric sensor is mounted on one side, close to the copper columns, of the upper surface of the angular contact ball bearing seat through a connecting plate; four liquid-transferring gun seats are circumferentially and uniformly distributed on the upper end surface of the liquid-transferring gun plate close to the edge, each liquid-transferring gun seat is provided with a liquid-transferring gun with the lower end extending out of the liquid-transferring gun plate, a photoelectric sensor detection plate is fixed at the position of the lower end surface of the liquid-transferring gun plate corresponding to each liquid-transferring gun seat, and the photoelectric sensor detection plate is used for positioning the liquid-transferring gun seat after being contacted with a photoelectric sensor positioned below the photoelectric sensor detection plate; the liquid-transfering gun plate rotates around the center of the rotating shaft under the drive of the fourth rotating motor.
The gun head feeding module comprises a first moving motor 2.1, a first top plate, a first bottom plate, a first connecting plate, a first sliding block, a first optical axis, a first inverted L-shaped connecting plate, a gun barrel, a first rotating motor, a gun head feeding module connecting plate, a gun head disc and a gun disc seat; the liquid transferring gun comprises a liquid transferring gun module, a liquid transferring gun module and a liquid transferring gun module, wherein the liquid transferring gun module is fixed on a machine frame through a vertically arranged liquid transferring gun module connecting plate, the side surface of the first connecting plate is attached to the side surface of the liquid transferring gun module, the upper end surface and the lower end surface of the first connecting plate are respectively provided with a first top plate and a first bottom plate, a first optical axis is connected between the first top plate and the first bottom plate, the upper end surface and the lower end surface of the first bottom plate are provided with a first moving motor, the output shaft of the first moving motor penetrates out of the first bottom plate upwards to be connected with a first sliding block sleeved on the first optical axis, the vertical plate of the first inverted L, a gun head disc is fixed on the upper end face of the gun disc seat, a plurality of through holes are uniformly distributed on the end face of the gun head disc along the edge in the circumferential direction and used for placing a gun tube, a first baffle is fixed on the upper end face of the gun head disc and positioned between two of the through holes, and the upper end of the first baffle is used for being in contact with the upper end of a first initial positioning block to realize the initial positioning of the gun head disc; the first sliding block is driven by the first moving motor to move up and down along the first optical axis, so that the gun head disc connected with the first inverted L-shaped connecting plate is driven to move up and down, and the gun head disc rotates around the central shaft under the driving of the first rotating motor.
The upper liquid module comprises a second moving motor, a second top plate, a second initial positioning block, a second connecting plate, a second sliding block, a second optical axis, a second inverted L-shaped connecting plate, a second rotating motor, a first rotating disc, an upper liquid module connecting plate and a bottle seat; the upper liquid module is fixed on the frame through an upper liquid module connecting plate which is vertically arranged, the side surface of a second connecting plate is jointed with the side surface of the upper liquid module connecting plate facing the liquid-transfering gun module, a second top plate and a second bottom plate are respectively arranged on the upper end surface and the lower end surface of the second connecting plate, a second optical axis is connected between the second top plate and the second bottom plate, a second initial positioning block is arranged on the upper end surface of the second top plate, a second moving motor is arranged on the lower end surface of the second bottom plate, the output shaft of the second moving motor upwards penetrates through the second bottom plate to be connected with a second sliding block sleeved on the second optical axis, the vertical plate of the second inverted L-shaped connecting plate is connected with the side surface of the second sliding block far away from the second connecting plate, a second rotating motor is arranged on the lower end surface of the horizontal plate of the second inverted L-shaped connecting plate, the output shaft of the second rotating motor upwards sequentially penetrates, four bottle seats which are symmetrically arranged in pairs are fixed on the upper end face of the first rotating disc, and a reagent bottle is arranged in each bottle seat; a stop block is arranged at the upper part of each bottle seat and is used for contacting the upper end of a second initial positioning block to realize the initial positioning of the first rotary table; the second slider reciprocates along the second optical axis at the drive of second moving motor to drive and reciprocate with the first carousel of second shape of falling L connecting plate, first carousel is rotatory around the center pin under the drive of second rotating electrical machines.
The sample introduction module comprises a third moving motor, a third top plate, a third bottom plate, a third connecting plate, a third sliding block, a third optical axis, a third inverted L-shaped connecting plate, a third rotating motor, a third flange, a sample introduction module connecting plate, a second rotating disc, an open test tube 4.12, an open test tube rack 4.5, a second test tube 4.6, a second test tube rack and a third initial positioning block; the sample introduction module is fixed on the frame through a sample introduction module connecting plate which is vertically arranged, the side surface of a third connecting plate is jointed with the side surface of the sample introduction module connecting plate facing to the liquid-transfering gun module, the upper end surface and the lower end surface of the third connecting plate are respectively provided with a third top plate and a third bottom plate, a third optical axis is connected between the third top plate and the third bottom plate, the upper end surface of the third top plate is provided with a third initial positioning block, the lower end surface of the third bottom plate is provided with a third moving motor, the output shaft of the third moving motor penetrates upwards through the third bottom plate and is connected with a third sliding block sleeved on the third optical axis, the vertical plate of the third inverted L-shaped connecting plate is connected with the side surface of the third sliding block far away from the third connecting plate, the lower end surface of the horizontal plate of the third inverted L-shaped connecting plate is provided with a third rotating motor, the output shaft of the, a second test tube seat is fixed on one side of the upper end face of the second rotary table, a second test tube is placed in the middle of the second test tube seat, and a stop block is arranged on one side, away from the open test tube rack, of the upper portion of the second test tube seat and used for contacting with the upper end of a third initial positioning block to achieve initial positioning of the second rotary table; an opening test tube rack is mounted on the other side of the upper end face of the second rotary table, a cylindrical through hole for placing an opening test tube is formed in the upper portion of the opening test tube rack, a through groove penetrating through the opening test tube rack from front to back is formed in the lower portion of the opening test tube rack, and the lower end of the opening test tube extends out of the through hole and then is placed in the through groove; the third slider reciprocates along the third optical axis under the drive of third moving motor to drive and reciprocate with the second carousel of third shape of falling L connecting plate, the second carousel is rotatory around the center pin under the drive of third rotating electrical machines.
The magnetic separation module comprises a fourth moving motor, a front plate, a rear plate, a fourth connecting plate, a fourth sliding block, a fourth optical axis, a fourth L-shaped connecting plate, a magnet and a magnet fixing frame; the magnetic separation module is fixed on the rack through a fourth connecting plate which is horizontally arranged, a front plate and a rear plate are respectively fixed at the front end and the rear end of the fourth connecting plate, a fourth optical axis is connected between the front plate and the rear plate, a fourth moving motor is installed at one side of the front plate, which is far away from the pipette module, an output shaft of the fourth moving motor sequentially penetrates through the front plate to be connected with a fourth slider, the lower end face of a horizontal plate of a fourth L-shaped connecting plate is connected with the upper end face of the fourth slider, two magnet fixing frames which are arranged in a V shape are fixed on the side face, which is close to the pipette module, of a vertical plate of the fourth L-shaped connecting plate, and a magnet sleeve arranged at the front end; the fourth slider slides along the fourth optical axis under fourth moving motor's drive to the magnet mount that drives and link to each other with fourth L shape connecting plate removes towards opening test-tube rack's logical groove, makes two nested magnets of magnet mount front end remove to the outer wall both sides that are located logical inslot opening test tube.
The first liquid suction and discharge module and the second liquid suction and discharge module are both liquid suction and discharge modules, and each liquid suction and discharge module comprises a fifth moving motor, a fifth top plate, a fifth bottom plate, a fifth connecting plate, a liquid suction and discharge module connecting plate, a fifth sliding block, a fifth optical axis, a fifth L-shaped connecting plate and an ejector rod; the liquid suction and discharge module is fixed on the rack through a vertically arranged liquid suction and discharge module connecting plate, the side surface of a fifth connecting plate is attached to the side surface of the liquid suction and discharge module connecting plate facing the liquid-transfering gun module, a fifth top plate and a fifth bottom plate are respectively installed on the upper end surface and the lower end surface of the fifth connecting plate, a fifth optical axis is connected between the fifth top plate and the fifth bottom plate, a fifth moving motor is installed on the upper end surface of the fifth top plate, an output shaft of the fifth moving motor penetrates out of the fifth top plate downwards to be connected with a fifth sliding block sleeved on the fifth optical axis, a vertical plate of a fifth L-shaped connecting plate is connected with the side surface of the fifth sliding block close to the liquid-transfering gun module, and an ejector rod; the fifth sliding block slides along the fifth optical axis under the driving of a fifth moving motor, so that the ejector rod connected with the fifth L-shaped connecting plate is driven to move downwards to push the top end of the liquid-transfering gun for sucking or outputting liquid.
The gun head withdrawing module comprises a sixth moving motor, a sixth top plate, a sixth bottom plate, a sixth sliding block, a sixth optical axis, a sixth connecting plate, a seventh connecting plate, a gun head withdrawing connecting plate and an execution plate; the gun head withdrawing module is fixed on the rack through a vertically arranged gun head withdrawing connecting plate, the side surface of a sixth connecting plate is attached to the side surface of the gun head withdrawing connecting plate facing the liquid transferring gun module, a sixth top plate and a sixth bottom plate are respectively installed on the upper end surface and the lower end surface of the sixth connecting plate, a sixth optical axis is connected between the sixth top plate and the sixth bottom plate, a sixth moving motor is installed on the upper end surface of the sixth top plate, an output shaft of the sixth moving motor downwards penetrates through the sixth top plate to be connected with a sixth sliding block sleeved on the sixth optical axis, and an execution plate is connected with the side surface, close to the liquid transferring gun module, of the sixth sliding block through a; the sixth sliding block slides along a sixth optical axis under the driving of a sixth moving motor, so that the execution plate connected with the seventh connecting plate is driven to move downwards to push the push plate of the liquid transferring gun, and the gun barrel connected with the push plate is pushed to drop into the collecting tank below.
The four liquid-transfering guns are respectively a first liquid-transfering gun, a second liquid-transfering gun, a third liquid-transfering gun and a fourth liquid-transfering gun, one of the gun tubes in the gun head module is just aligned below the first liquid-transfering gun, one of the bottle seats in the liquid module is just aligned below the second liquid-transfering gun, an opening test tube (4.12) of the sample injection module is just aligned below the third liquid-transfering gun, and a collection tank is just aligned below the fourth liquid-transfering gun.
The center of the ejector rod of the first liquid suction and discharge module is positioned right above the second liquid transfer gun, and the center of the ejector rod of the second liquid suction and discharge module is positioned right above the third liquid transfer gun; and the center of an execution plate of the gun head withdrawing module is aligned with a push plate of a fourth pipetting gun right below the execution plate.
The end faces of two opposite poles of the magnet are isolated from the outside through a magnetic sleeve and are used for preventing strong attraction generated by mutual pairing of N-S poles; the left and right end faces of the magnet are not isolated from the outside through the magnetic sleeves.
Secondly, pretreatment method for chicken extracting solution by adopting device
The method comprises the following steps:
s1: the gun barrel is placed on the gun head disc, the first to fourth reagent bottles are placed in the four bottle seats respectively, magnetic bead solution is filled in the first reagent bottle, PBS-T solution is filled in the second reagent bottle, PBS-BSA is filled in the third reagent bottle, and quantum dot solution is filled in the fourth reagent bottle. Putting the chicken extract solution into an open test tube in advance, putting the open test tube into an open test tube rack, putting a second test tube into a second test tube rack, returning each module to an initial position, and adjusting the range required by the liquid-transfering gun;
s2: loading a gun barrel; the gun head module is lifted up to enable the gun barrel of the gun head disc to be sleeved on the liquid transferring gun, and then the gun head module is lifted down to ensure that the descending distance is longer than the length of the gun barrel;
s3: rotating the rotating shaft by 90 degrees to enable the first liquid-transfering gun to rotate above the upper liquid module, enabling the fourth liquid-transfering gun to rotate above the gun head entering module, enabling the gun head disc to rotate by the gun head entering module to enable the gun barrel to face the fourth liquid-transfering gun and ascend, and enabling the fourth liquid-transfering gun to finish the operation of assembling the gun barrel in the step S2;
s4: liquid feeding operation: the upper liquid module ascends, a first reagent bottle is immersed under the barrel of the first liquid-transferring gun, the first liquid-absorbing and discharging module presses the piston of the first liquid-transferring gun downwards through the ejector rod, then the ejector rod ascends, and at the moment, the first liquid-transferring gun absorbs the magnetic bead solution with the required measuring range;
s5: the rotating shaft rotates by 90 degrees, so that the first liquid-transferring gun rotates to the position above the sample feeding module, the fourth liquid-transferring gun rotates to the position above the upper liquid module, the third liquid-transferring gun rotates to the position above the liquid feeding head module, the liquid feeding head module rotates the gun head disc to enable the gun barrel to be over against the third liquid-transferring gun and ascend, the third liquid-transferring gun completes S2 gun tube loading operation, and at the moment, the fourth liquid-transferring gun does not absorb any liquid;
s6: the second liquid suction and discharge module compresses a piston of the first liquid transfer gun through the ejector rod, so that the magnetic bead solution in the first liquid transfer gun is output to the open test tube;
s7: rotating the rotating shaft by 90 degrees to enable the first liquid transferring gun to rotate to the position below the gun head withdrawing module, rotating the fourth liquid transferring gun to the position above the open test tube, rotating the third liquid transferring gun to the position above the upper liquid module, rotating the first rotating shaft of the upper liquid module by 90 degrees, aligning the second reagent bottle to the third liquid transferring gun, and completing the liquid operation in the step S4, wherein the third liquid transferring gun absorbs the PBS-T solution with the required range; the sample introduction module ascends to enable a gun tube of the fourth pipetting gun to contact the bottom of the open test tube, and the ejector rod of the second pipetting module is controlled to ascend and descend continuously to enable liquid in the fourth pipetting gun to be sucked and output continuously, so that the magnetic bead solution and the chicken extracting solution in the open test tube are mixed uniformly; after uniform mixing, the sample injection module is lowered to an initial position; moving the execution plate of the gun head withdrawing module downwards to push the push plate of the first liquid transferring gun to move so as to drive the gun barrel to fall into the collecting tank below;
s8: rotating a second turntable of the sample introduction module, transferring the open test tube rack to a magnet fixing rack aligned with the magnetic separation module, controlling the two magnet fixing racks of the magnetic separation module to move forwards to two sides of the outer wall of the open test tube, after standing, concentrating magnetic beads in the open test tube on the wall of the test tube, moving the magnet fixing racks backwards, and then rotating the second turntable of the sample introduction module by 90 degrees to an initial position;
s9: controlling the sample introduction module to ascend to enable a barrel of a fourth liquid transfer gun to contact the bottom of the open test tube, controlling a mandril of the second liquid suction and discharge module to descend and ascend, and sucking the separated clear liquid in the open test tube into the barrel of the fourth liquid transfer gun;
s10: the rotating shaft rotates by 90 degrees, the third liquid transferring gun is transferred to the position above the sample feeding module, the fourth liquid transferring gun is transferred to the position below the gun head withdrawing module, and the second liquid transferring gun is transferred to the position above the upper liquid module; the first liquid-transfering gun is transferred to the upper part of the gun head feeding module, the gun tube loading operation of the step S2 is completed, and at the moment, the second liquid-transfering gun does not absorb any liquid; the second liquid suction and discharge module compresses a piston of the third liquid transfer gun through the ejector rod, so that the PBS-T solution in the third liquid transfer gun is discharged to the open test tube; moving the execution plate of the gun head withdrawing module downwards to push the push plate of the fourth liquid transferring gun 1.4, so that the gun barrel falls into the collecting tank below;
s11: the rotating shaft rotates by 90 degrees, so that the third liquid transferring gun is transferred to the position below the gun head withdrawing module, and the second liquid transferring gun is transferred to the position above the sample feeding module; the fourth liquid transferring gun is transferred to the position above the gun head feeding module, and the first liquid transferring gun is transferred to the position above the upper liquid module; at this time, the first pipette does not absorb any liquid; the sample introduction module ascends to enable a gun tube of the second pipette to contact the bottom of the open test tube, and liquid in the open test tube is continuously sucked and discharged by controlling the ejector rod of the second pipette module to continuously ascend and descend, so that the PBS-T solution and the magnetic beads after magnetic separation are uniformly mixed; moving the execution plate of the gun head withdrawing module downwards to push a push plate of a third liquid transferring gun, so that the gun barrel falls into a collecting tank below; the gun head feeding module rotates and raises the gun head disc, so that the fourth liquid-transferring gun completes the gun tube loading operation of the step S2;
s12: after the liquid in the open test tube is uniformly mixed, performing the magnetic separation operation of step S8;
s13: the rotating shaft rotates by 90 degrees, so that the first liquid transferring gun is transferred to the upper part of the sample introduction module, the fourth liquid transferring gun is transferred to the upper part of the upper liquid module, the third liquid transferring gun is transferred to the upper part of the gun head entering module 2, and the second liquid transferring gun is transferred to the lower part of the gun head withdrawing module;
controlling the sample introduction module to ascend to enable the barrel of the first liquid transfer gun to contact the bottom of the open test tube, compressing the first liquid transfer gun by controlling the ejector rod of the second liquid suction and discharge module and then ascending to enable separated clear liquid to be sucked into the barrel of the first liquid transfer gun; an execution plate of the gun head withdrawing module moves downwards to push a push plate of a second liquid transferring gun to move, so that a gun barrel falls into a collecting tank below; the gun head feeding module rotates the gun head disc to enable the third liquid-transferring gun to face an unused gun barrel, and the gun head disc is lifted to enable the third liquid-transferring gun to complete gun tube loading operation in the step S2;
the first rotating disc of the liquid feeding module rotates by 90 degrees, so that the gun tube of the fourth liquid transferring gun is aligned to the third reagent bottle, the first liquid sucking and discharging module compresses the fourth liquid transferring gun through the ejector rod, the ejector rod rises, and the fourth liquid transferring gun absorbs PBS-BSA liquid with the required range;
s14: the rotating shaft rotates by 90 degrees, so that the first liquid transferring gun is transferred to the position below the gun head withdrawing module, the second liquid transferring gun is transferred to the position above the gun head feeding module, the third liquid transferring gun is transferred to the position above the upper liquid module, and the fourth liquid transferring gun is transferred to the position above the sample feeding module;
an execution plate of the gun head withdrawing module moves downwards to push a push plate of the first liquid transferring gun to move downwards, so that a gun barrel is pushed to fall into a collecting tank below; the gun head feeding module rotates the gun head disc to enable the second liquid-transferring gun to face an unused gun barrel, and the gun head disc is lifted to enable the second liquid-transferring gun to complete gun tube loading operation in the step S2; the sample introduction module ascends to enable the barrel of the fourth liquid transfer gun to contact the bottom of the open test tube, the ejector rod of the second liquid suction and discharge module is controlled to ascend and descend continuously, liquid in the open test tube is sucked and discharged continuously, the PBS-BSA solution in the open test tube and the solution after magnetic separation in the step S13 are mixed uniformly, and the sample introduction module descends to an initial position;
the first rotating disc of the liquid feeding module rotates by 90 degrees, so that the reagent bottle No. four is aligned to the third liquid transferring gun, the first liquid sucking and discharging module compresses a piston of the third liquid transferring gun downwards through the ejector rod, the ejector rod rises, and the third liquid transferring gun absorbs the quantum dot solution with the required measuring range;
s15: the rotating shaft rotates by 90 degrees, the first liquid transferring gun is transferred to the position above the gun head feeding module, the second liquid transferring gun is transferred to the position above the upper liquid module, the third liquid transferring gun 1.3 is transferred to the position above the sample feeding module, and the fourth liquid transferring gun is transferred to the position below the gun head withdrawing module;
the gun head feeding module rotates the gun head disc to enable the first liquid-transferring gun to face an unused gun barrel, and raises the gun head disc to enable the first liquid-transferring gun to finish the gun tube loading operation in the step S2; the execution plate of the gun head withdrawing module moves downwards to push the push plate of the fourth liquid transferring gun to move, so that the gun barrel below the push plate is pushed to fall into the collecting tank;
controlling the sample introduction module to ascend so that a gun barrel of the third liquid transfer gun contacts the bottom of the open test tube, controlling the ejector rod of the second liquid suction and discharge module to continuously ascend and descend, so that liquid in the open test tube is continuously sucked and discharged, uniformly mixing the quantum dot solution, the PBS-BSA solution and the solution subjected to the magnetic separation in the step S13 in the open test tube, and descending the sample introduction module to an initial position;
s16: after the liquid in the open test tube is uniformly mixed, carrying out S8 magnetic separation operation;
s17: the rotating shaft rotates by 90 degrees, so that the first liquid transferring gun is transferred to the upper part of the upper liquid module, the second liquid transferring gun is transferred to the sample introduction module, the third liquid transferring gun is transferred to the lower part of the gun head withdrawing module, and the fourth liquid transferring gun is transferred to the upper part of the gun head entering module;
controlling the sample introduction module to ascend to enable a gun tube of the second liquid transfer gun to contact the bottom of the open test tube, and controlling an ejector rod of the second liquid suction and discharge module to compress the second liquid transfer gun downwards and then descend, so that the ejector rod ascends to enable separated clear liquid to be sucked into the second liquid transfer gun;
moving the execution plate of the gun head withdrawing module downwards to push a push plate of a third liquid transferring gun, so that a gun barrel below the push plate is pushed to fall into the collecting tank; the gun head feeding module rotates the gun head disc to enable the fourth liquid-transferring gun to face an unused gun barrel, and the gun head disc is lifted to enable the fourth liquid-transferring gun to complete gun tube loading operation in the step S2;
the third reagent bottle is aligned to the first liquid transferring gun by rotating the first rotary disc of the upper liquid module, the first liquid sucking and discharging module downwards compresses a piston of the first liquid transferring gun through a mandril, the mandril ascends, and the first liquid transferring gun absorbs PBS-BSA liquid with a required measuring range;
s18: the rotating shaft rotates by 90 degrees, so that the second liquid transferring gun is transferred to the position below the gun head withdrawing module, the first liquid transferring gun is transferred to the position above the sample feeding module, the third liquid transferring gun is transferred to the gun head feeding module, and the fourth liquid transferring gun is transferred to the position above the upper liquid module 3;
the sample introduction module ascends to enable a gun tube of the first liquid transfer gun to contact the bottom of the open test tube, the ejector rod of the second liquid suction and discharge module is controlled to ascend and descend continuously, liquid in the open test tube is sucked and discharged continuously, the PBS-BSA solution and the solution after magnetic separation in the step S18 are mixed uniformly, and the sample introduction module descends to an initial position;
moving the execution plate of the gun head withdrawing module downwards, and pushing the push plate of the second liquid transferring gun to enable the gun barrel connected with the push plate to fall into the collecting tank;
s19: the rotating shaft rotates by 90 degrees, so that the first liquid transferring gun is transferred to the position below the gun head withdrawing module, the second liquid transferring gun is transferred to the position above the gun head advancing module, the third liquid transferring gun is transferred to the position above the upper liquid module, and the fourth liquid transferring gun 1.4 is transferred to the position above the sample introducing module; the sampling module ascends to enable a gun tube of the fourth pipetting gun to contact the bottom of the open test tube, the ejector rod of the second liquid suction and discharge module is controlled to compress the fourth pipetting gun downwards, then the ejector rod ascends, and at the moment, the fourth pipetting gun absorbs liquid in the open test tube with the required measuring range;
a second rotating disc of the sample introduction module rotates 180 degrees, so that a second test tube rack is aligned to a fourth liquid transfer gun, and then the second liquid suction and discharge module is compressed to the fourth liquid transfer gun through a mandril to discharge liquid in the liquid transfer gun;
a second rotating disc of the sample injection module rotates by 180 degrees, and a second test tube 4.6 is taken out for subsequent detection of the microplate reader/spectrometer;
s20: the rotating shaft rotates by 90 degrees, so that the fourth liquid gun is rotated to the position below the gun head withdrawing module, the third liquid transferring gun is rotated to the position above the sample feeding module, the second liquid transferring gun is rotated to the position above the upper liquid module, and the first liquid transferring gun is rotated to the position above the gun head feeding module;
s21: and resetting each module to the initial position, and ending.
The initial position of each module is: the initial position of the pipette head module is that the upper end of a first baffle plate and the upper end of a first initial positioning block are contacted and superposed, the initial position of the upper liquid module is that a stop block arranged on the upper part of a bottle seat is contacted with the upper end of a second initial positioning block, the initial position of the sample feeding module is that the stop block on the upper part of a second test tube seat is contacted with the upper end of a third initial positioning block, the initial position of the pipette head module is that a photoelectric sensor 1.5 photosensitive center aligns with one photoelectric sensor detection plate, the initial position of the pipette head module is that a seventh slide block moves to the upper end face of a sixth top plate, and the initial position of the pipette head module is that a fifth slide block moves.
The invention has the beneficial effects that:
the invention realizes the automation of the mixing and magnetic separation of the magnetic particles at the nanometer level; and the automatic pretreatment of the detection sample is realized, and the mixing speed, the magnetic bead adsorption efficiency and the automation efficiency of the sample to be detected can be improved.
Drawings
Fig. 1 is a perspective view of the device of the present invention.
Fig. 2 is a front view of the device of the present invention.
Fig. 3 is a side view of fig. 2.
Fig. 4 is a top view of fig. 2.
FIG. 5 is a front view of a pipette module of the present invention.
Fig. 6 is a side view of fig. 5.
Fig. 7 is a top view of fig. 5.
FIG. 8 is a front view of a sample introduction module of the present invention
FIG. 9 is a side view of FIG. 8
FIG. 10 is a front view of a liquid module of the present invention
FIG. 11 is a side view of FIG. 10
FIG. 12 is a top view of FIG. 10
FIG. 13 is a perspective view of the magnetic separation module of the present invention in an operational state
FIG. 14 is a front view of the magnetic separation module of the present invention in an operating state
FIG. 15 is a perspective view of a tip retracting module of the present invention
FIG. 16 is a perspective view of a liquid suction and discharge module according to the present invention
FIG. 17 is a front view of a lance tip module of the present invention
FIG. 18 is a side view of FIG. 17
FIG. 19 is a schematic view of the construction of the pipette of this invention
In the figure: 1, a liquid-transfering gun module; 2, a gun head feeding module; 3, a liquid module; 4, a sample introduction module; 5, withdrawing the gun head module; 6 a first liquid suction and discharge module; 7 a second liquid suction and discharge module; 8, a collection tank; 9 a magnetic separation module;
1.1 a first pipette; 1.2 second pipetting gun; 1.3 a third pipetting gun; 1.4 fourth pipetting gun; 1.5 a photosensor; 1.6 photoelectric sensor detecting board; 1.7 copper columns; 1.8 supporting plates; 1.9 liquid-transfering gun module bottom plate; 1.10 a fourth rotating electrical machine; 1.11 angular contact ball bearing seats; 1.12 pipette plate; 1.13 rotating shaft; 1.14 pipette tip holder; 1.15 motor connecting plate; 1.16 shaft couplings; 1.17 angular contact bearing seat
2.1 a first moving motor; 2.2 front panel; 2.3 first slide block; 2.4 a first optical axis; 2.5 a first connecting plate; 2.6 a first primary locating block; 2.7 a bottom plate; 2.8 gun barrel; 2.9 a first flange; 2.10 a first rotating electrical machine; 2.11 gun head disk; 2.12 connecting plates of the feeding gun head module; 2.13 first L-shaped connecting plate; 2.14 gun plate seat
3.1 reagent bottle No. one; 3.2 reagent bottle II; 3.3 reagent bottle III; 3.4 reagent bottle No. four; 3.5 a second rotating electrical machine; 3.6 second L-shaped connecting plate; 3.7 second flange; 3.8 a second primary positioning block; 3.9 a second optical axis; 3.10 second slide block; 3.11 second moving motor; 3.12 liquid module connecting plates are arranged; 3.13 front panel; 3.14 a first carousel; 3.15 second connecting plate; 3.16 bottom plate; 3.17 bottle base
4.1 a third moving motor; 4.2 a third rotating electrical machine; 4.3 third flange; 4.4 second carousel; 4.5 opening the test tube rack; 4.6 second test tube; 4.7 a third primary locating block; 4.8 second test tube rack; 4.9 third optical axis; 4.10 third connecting plate; 4.11 third slide block; 4.12 open test tubes; 4.13 sample module connecting plate; 4.14 third L-shaped connecting plate; 4.15 front panel; 4.16 rear panel
5.1 a seventh moving motor; 5.2 eighth connecting plate; 5.3 execution board; 5.4 seventh optical axis; 5.5 a bottom plate; 5.6 a seventh connecting plate; 5.7 withdrawing the connecting plate of the gun head; 5.8 seventh slide; 5.9 front Panel
6.1 a fifth moving motor; 6.2 front panel; 6.3 a fifth L-shaped connecting plate; 6.4 first ejector pin; 6.5 fifth optical axis; 6.6 a bottom plate; 6.7 a fifth connecting plate; 6.8 first suction/discharge connection plate; 6.9 fifth slider
9.1 a fourth moving motor; 9.2 front panel; 9.3 a fourth connecting plate; 9.4 a bottom plate; 9.5 a magnet fixing frame; 9.6 fourth L connecting plate; 9.7 fourth slide block; 9.8 fourth optical axis; 9.9 magnet
Position Q1; q2 position number two; q3: a spring; q4: pushing the plate; q5: pipette bottom Q6: piston
Detailed Description
The invention is further illustrated by the following figures and examples.
As shown in fig. 1, 2 and 3, the invention comprises a frame, a pipette module 1, a pipette head module 2, an upper liquid module 3, a sample introduction module 4, a pipette head withdrawing module 5, a first liquid suction and discharge module 6, a second liquid suction and discharge module 7, a collection tank 8 and a magnetic separation module 9, wherein the pipette module 1 is installed in the middle of the frame, the pipette head module 2, the upper liquid module 3, the sample introduction module 4 and the collection tank 8 are respectively installed at the bottom of the frame and around the pipette module 1 through the frame, the pipette head module 2 and the sample introduction module 4 are arranged oppositely, the upper liquid module 3 and the collection tank 8 are arranged oppositely, and the magnetic separation module 9 is installed at one side of the sample introduction module 4 close to the upper liquid module 3 through the frame; go up liquid module 3 top and be located the frame top and install first liquid absorption and drainage module 6, advance kind module 4 top and be located the frame top and install second liquid absorption and drainage module 7, be located the frame top and install and move back rifle head module 5.
As shown in fig. 5 and 6, the pipetting gun module 1 comprises a pipetting gun, a pipetting gun seat 1.14, a pipetting gun tray 1.12, a pipetting gun module bottom plate 1.9, a motor connecting plate 1.15, an angular contact ball bearing seat 1.11, a fourth rotating motor 1.10, a rotating shaft 1.13, a photoelectric sensor 1.5 and a photoelectric sensor detection plate 1.6; a bottom plate 1.9 of the liquid-transferring gun module is fixed at the center of the bottom of the frame, a motor connecting plate 1.15 is fixed above the bottom plate 1.9 of the liquid-transferring gun module through two supporting plates 1.8, a fourth rotating motor 1.10 is arranged on the lower surface of the motor connecting plate 1.15, and an output shaft of the fourth rotating motor 1.10 penetrates out of the motor connecting plate 1.15 upwards and is connected with a coupler 1.16; an angular contact ball bearing seat 1.11 is fixed above the upper surface of a motor connecting plate 1.15 through two copper columns 1.7, a rotating shaft 1.13 is installed in the middle of the angular contact ball bearing seat 1.11 through an angular contact ball bearing 1.17, a liquid-transferring gun plate 1.12 is installed at the upper end of the rotating shaft 1.13, the lower end of the rotating shaft 1.13 is connected with an output shaft of a fourth rotating motor 1.10 through a coupler 1.16, and a photoelectric sensor 1.5 is installed on one side, close to the copper columns 1.7, of the upper surface of the angular contact ball bearing seat 1.11 through a connecting plate; four liquid-transfering gun seats 1.14 are circumferentially and uniformly distributed at the position, close to the edge, of the upper end face of the liquid-transfering gun tray 1.12, each liquid-transfering gun seat 1.14 is provided with a liquid-transfering gun of which the lower end extends out of the liquid-transfering gun tray 1.12, the position of the lower end face of the liquid-transfering gun tray 1.12 corresponding to each liquid-transfering gun seat 1.14 is fixedly provided with a photoelectric sensor detection plate 1.6, and the photoelectric sensor detection plate 1.6 is used for realizing the positioning of the liquid-transfering gun seats 1.14 after contacting with a photoelectric sensor 1.5 positioned below the photoelectric sensor; the pipette tip tray 1.12 is driven by the fourth rotating motor 1.10 to rotate around the center of the rotating shaft 1.13.
As shown in fig. 7, the four pipette guns are respectively a first pipette gun 1.1, a second pipette gun 1.2, a third pipette gun 1.3 and a fourth pipette gun 1.4, one barrel 2.8 of the pipette head module 2 is aligned under the first pipette gun 1.1, one bottle seat 3.17 of the upper liquid module 3 is aligned under the second pipette gun 1.2, an opening test tube 4.12 of the sample injection module 4 is aligned under the third pipette gun 1.3, and a collection tank 8 is aligned under the fourth pipette gun 1.4.
As shown in fig. 8 and 9, the sample module 4 includes a third moving motor 4.1, a third top plate 4.16, a third bottom plate 4.15, a third connecting plate 4.10, a third slider 4.11, a third optical axis 4.9, a third inverted L-shaped connecting plate 4.14, a third rotating motor 4.2, a third flange 4.3, a sample module connecting plate 4.13, a second rotating disk 4.4, an open test tube 4.12, an open test tube rack 4.5, a second test tube 4.6, a second test tube rack 4.8, and a third initial positioning block 4.7; the sample introduction module 4 is fixed on the frame through a sample introduction module connecting plate 4.13 which is vertically arranged, the side surface of a third connecting plate 4.10 is attached to the side surface of the sample introduction module connecting plate 4.13 facing the liquid transfer gun module 1, the upper end surface and the lower end surface of the third connecting plate 4.10 are respectively provided with a third top plate 4.16 and a third bottom plate 4.15, a third optical axis 4.9 is connected between the third top plate 4.16 and the third bottom plate 4.15, the upper end surface of the third top plate 4.16 is provided with a third initial positioning block 4.7, the lower end surface of the third bottom plate 4.15 is provided with a third moving motor 4.1, the output shaft of the third moving motor 4.1 upwards penetrates through the third bottom plate 4.15 to be connected with a third sliding block 4.11 sleeved on the third optical axis 4.9, the vertical plate of a third inverted L-shaped connecting plate 4.14 is connected with the side surface of the third sliding block 4.11 far away from the third connecting plate 4.10, the lower end surface of the horizontal plate of the third inverted L-shaped connecting plate 4.14 is provided with a third rotating motor 4.2, and the horizontal plate, The rear part of the third flange 4.3 is connected with a second rotary table 4.4, one side of the upper end face of the second rotary table 4.4 is fixedly provided with a second test tube rack 4.8, a second test tube 4.6 is placed in the middle of the second test tube rack 4.8, and one side of the upper part of the second test tube rack 4.8, which is far away from the opening test tube rack 4.5, is provided with a stop block for contacting with the upper end of a third initial positioning block 4.7 to realize the initial positioning of the second rotary table 4.4; an open test tube rack 4.5 is installed on the other side of the upper end face of the second rotary table 4.4, a cylindrical through hole for placing an open test tube 4.12 is formed in the upper portion of the open test tube rack 4.5, a through groove penetrating through the lower portion of the open test tube rack 4.5 in the front-back direction is formed in the lower portion of the open test tube rack 4.12, and the lower end of the open test tube 4.12 extends out of the through hole and; the third sliding block 4.11 is driven by the third moving motor 4.1 to move up and down along the third optical axis 4.9, so as to drive the second rotating disc 4.4 connected with the third inverted-L-shaped connecting plate 4.14 to move up and down, and the second rotating disc 4.4 is driven by the third rotating motor 4.2 to rotate around the central shaft.
As shown in fig. 10 and 11, the upper liquid module 3 includes a second moving motor 3.11, a second top plate 3.16, a second initial positioning block 3.8, a second connecting plate 3.15, a second slider 3.10, a second optical axis 3.9, a second inverse L-shaped connecting plate 3.6, a second rotating motor 3.5, a first rotating disk 3.14, an upper liquid module connecting plate 3.12, and a bottle holder 3.17; the upper liquid module 3 is fixed on the rack through an upper liquid module connecting plate 3.12 which is vertically arranged, the side surface of a second connecting plate 3.15 is attached to the side surface of the upper liquid module connecting plate 3.12 facing the liquid-moving gun module 1, a second top plate 3.16 and a second bottom plate 3.13 are respectively installed on the upper end surface and the lower end surface of the second connecting plate 3.15, a second optical axis 3.9 is connected between the second top plate 3.16 and the second bottom plate 3.13, a second initial positioning block 3.8 is installed on the upper end surface of the second top plate 3.16, a second moving motor 3.11 is installed on the lower end surface of the second bottom plate 3.13, an output shaft of the second moving motor 3.11 upwards penetrates through the second bottom plate 3.13 to be connected with a second sliding block 3.10 sleeved on the second optical axis 3.9, a vertical plate of the second inverted L-shaped connecting plate 3.6 is connected with the side surface of the second sliding block 3.10 far away from the second connecting plate 3.15, a second rotating motor 3.5 is installed on the lower end surface of a horizontal plate of the second inverted L-shaped connecting plate 3.6, and a horizontal plate of the second rotating, The rear part of the second flange 3.7 is connected with a first rotating disc 3.14, four bottle seats 3.17 which are symmetrically arranged in pairs are fixed on the upper end surface of the first rotating disc 3.14, and reagent bottles are arranged in each bottle seat 3.17; a stop block is arranged at the upper part of each bottle seat 3.17 and is used for contacting the upper end of the second initial positioning block 3.8 to realize the initial positioning of the first rotary disc 3.14; the second sliding block 3.10 is driven by the second moving motor 3.11 to move up and down along the second optical axis 3.9, so as to drive the first rotating disc 3.14 connected with the second inverted-L-shaped connecting plate 3.6 to move up and down, and the first rotating disc 3.14 is driven by the second rotating motor 3.5 to rotate around the central shaft.
As shown in fig. 13, the magnetic separation module 9 includes a fourth moving motor 9.1, a front plate 9.2, a rear plate 9.4, a fourth connecting plate 9.3, a fourth slider 9.7, a fourth optical axis 9.8, a fourth L-shaped connecting plate 9.6, a magnet 9.9, and a magnet fixing frame 9.5; the magnetic separation module 9 is fixed on the rack through a fourth connecting plate 9.3 which is horizontally arranged, a front plate 9.2 and a rear plate 9.4 are respectively fixed at the front end and the rear end of the fourth connecting plate 9.3, a fourth optical axis 9.8 is connected between the front plate 9.2 and the rear plate 9.4, a fourth moving motor 9.1 is installed at one side of the front plate 9.2 far away from the pipette module 1, an output shaft of the fourth moving motor 9.1 sequentially penetrates through the front plate 9.2 to be connected with a fourth slider 9.7, the lower end face of a horizontal plate of the fourth L-shaped connecting plate 9.6 is connected with the upper end face of the fourth slider 9.7, two magnet fixing frames 9.5 which are arranged in a V shape are fixed on the side face of a vertical plate of the fourth L-shaped connecting plate 9.6 close to the pipette module 1, and a magnet 9.9 is nested in a magnetic sleeve arranged at the front end of each magnet fixing frame 9; fourth slider 9.7 slides along fourth optical axis 9.8 under the drive of fourth mobile motor 9.1 to the magnet mount 9.5 that the drive links to each other with fourth L shape connecting plate 9.6 moves towards opening test-tube rack 4.5's logical groove, makes two magnet mount 9.5 front end nested magnet 9.9 remove to the outer wall both sides that are located logical inslot opening test tube 4.12.
As shown in fig. 14, the end faces of the two poles of the magnet 9.9 opposite to each other are isolated from the outside by the magnetic sleeve, so as to prevent the strong attraction force generated by the mutual N-S poles; the left end face and the right end face of the magnet 9.9 are not isolated from the outside through the magnetic sleeves.
As shown in fig. 15, the gun head withdrawing module 5 includes a sixth moving motor 5.1, a sixth top plate 5.9, a sixth bottom plate 5.5, a seventh slider 5.8, a sixth optical axis 5.4, a sixth connecting plate 5.6, a seventh connecting plate 5.2, a gun head withdrawing connecting plate 5.7, and an execution plate 5.3; the gun head withdrawing module 5 is fixed on the rack through a vertically arranged gun head withdrawing connecting plate 5.7, the side surface of a sixth connecting plate 5.6 is attached to the side surface of the gun head withdrawing connecting plate 5.7 facing the liquid transferring gun module 1, the upper end surface and the lower end surface of the sixth connecting plate 5.6 are respectively provided with a sixth top plate 5.9 and a sixth bottom plate 5.5, a sixth optical axis 5.4 is connected between the sixth top plate 5.9 and the sixth bottom plate 5.5, the upper end surface of the sixth top plate 5.9 is provided with a sixth moving motor 5.1, the output shaft of the sixth moving motor 5.1 penetrates downwards through the sixth top plate 5.9 to be connected with a seventh sliding block 5.8 sleeved on the sixth optical axis 5.4, and the execution plate 5.3 is connected with the side surface of the seventh sliding block 5.8 close to the liquid transferring gun module 1 through a seventh connecting plate 5.; the seventh slider 5.8 slides along the sixth optical axis 5.4 under the drive of the sixth mobile motor 5.1, thereby driving the execution plate 5.3 connected with the seventh connecting plate 5.2 to move downwards for pushing the push plate of the pipette, and further pushing the barrel 2.8 connected with the push plate to drop into the collecting tank 8 below.
As shown in fig. 4, the first liquid suction and discharge module 6 and the second liquid suction and discharge module 7 are both liquid suction and discharge modules, and as shown in fig. 16, each liquid suction and discharge module includes a fifth moving motor 6.1, a fifth top plate 6.2, a fifth bottom plate 6.6, a fifth connecting plate 6.7, a liquid suction and discharge module connecting plate 6.8, a fifth slider 6.9, a fifth optical axis 6.5, a fifth L-shaped connecting plate 6.3, and a push rod 6.4; the liquid suction and drainage module is fixed on the rack through a vertically arranged liquid suction and drainage module connecting plate 6.8, the side surface of a fifth connecting plate 6.7 is attached to the side surface of the liquid suction and drainage module connecting plate 6.8 facing the liquid transfer gun module 1, a fifth top plate 6.2 and a fifth bottom plate 6.6 are respectively installed on the upper end surface and the lower end surface of the fifth connecting plate 6.7, a fifth optical axis 6.5 is connected between the fifth top plate 6.2 and the fifth bottom plate 6.6, a fifth moving motor 6.1 is installed on the upper end surface of the fifth top plate 6.2, an output shaft of the fifth moving motor 6.1 penetrates downwards through the fifth top plate 6.2 to be connected with a fifth sliding block 6.9 sleeved on the fifth optical axis 6.5, a vertical plate of a fifth L-shaped connecting plate 6.3 is connected with the side surface, close to the liquid transfer gun module 1, of the fifth sliding block 6.9, and a mandril 6.4 is installed on the lower; the fifth slide block 6.9 is driven by the fifth moving motor 6.1 to slide along the fifth optical axis 6.5, so as to drive the ejector rod 6.4 connected with the fifth L-shaped connecting plate 6.3 to move downwards for pushing the top end of the pipette, and sucking or outputting liquid.
As shown in fig. 17 and 18, the gun head entering module 2 includes a first moving motor 2.1, a first top plate 2.7, a first bottom plate 2.2, a first connecting plate 2.5, a first slider 2.3, a first optical axis 2.4, a first inverted L-shaped connecting plate 2.13, a gun barrel 2.8, a first rotating motor 2.10, a gun head entering module connecting plate 2.12, a gun head disc 2.11 and a gun disc seat 2.14; the liquid transferring gun comprises a liquid transferring gun module 1, a liquid transferring gun module 2, a first connecting plate 2.5, a first top plate 2.7, a first bottom plate 2.2, a first optical axis 2.4, a first initial positioning block 2.6, a first movable motor 2.1, a first sliding block 2.3, a first inverted L-shaped connecting plate 2.13, a first rotating motor 2.10, a first inverted L-shaped connecting plate 2.13, a second rotating motor 2.13, a second sliding block 2.3, a first inverted L-shaped connecting plate 2.13, a second rotating motor 2.10, a second rotating motor 2.13, a second rotating motor 2.2.2, a second rotating motor and a second rotating motor, wherein the first rotating motor 2.5, the second rotating motor 2.7 and, The rear end of the first flange 2.9 is connected with a gun plate seat 2.14, a gun head plate 2.11 is fixed on the upper end face of the gun plate seat 2.14, a plurality of through holes are uniformly distributed on the end face of the gun head plate 2.11 along the edge circumference and used for placing a gun tube 2.8, a first baffle plate 2.15 is fixed on the upper end face of the gun head plate 2.11 and positioned between the two through holes, and the upper end of the first baffle plate 2.15 is used for contacting with the upper end of a first initial positioning block 2.6 to realize the initial positioning of the gun head plate 2.11; the first slide block 2.3 is driven by the first moving motor 2.1 to move up and down along the first optical axis 2.4, so as to drive the gun head disc 2.11 connected with the first inverted-L-shaped connecting plate 2.13 to move up and down, and the gun head disc 2.11 is driven by the first rotating motor 2.10 to rotate around the central shaft.
As shown in fig. 19, which is a structural diagram of a pipette gun, the pipette gun used in the present invention is a common professional fluid apparatus known in the art, and the working principle thereof is as follows: after the specific value of the measuring range is set, the spring Q3 expands and contracts to exhaust part of air, the liquid is sucked by atmospheric pressure, and the piston Q6 pushes the exhausted liquid. The piston of the pipette has two working positions Q1 and Q2. When pushed to the first position Q1, the gun barrel is used for exhausting air and absorbing liquid, and when pushed to the second position Q2, the gun barrel is used for exhausting liquid in the gun barrel. The barrel may be inserted into the pipette bottom Q5 in alignment and may be pushed out by pushing the pusher plate Q4.
The specific implementation method of the invention comprises the following steps:
s1: a gun barrel 2.8 is placed on a gun head plate 2.11, a first reagent bottle to a fourth reagent bottle shown in fig. 12 are respectively placed in four bottle seats 3.17, a magnetic bead solution is filled in the first reagent bottle 3.1, a PBS-T solution (containing Tween) is filled in the second reagent bottle 3.2, a PBS-BSA (bovine serum albumin) is filled in the third reagent bottle 3.3, and a quantum dot solution is filled in the fourth reagent bottle 3.4. Putting the chicken extract solution into an open test tube 4.12 in advance, putting the open test tube 4.12 into an open test tube rack 4.5, putting a second test tube 4.6 into a second test tube rack 4.8, returning each module to an initial position, and adjusting the range required by the liquid-transfering gun;
the initial positions of the modules are: the initial position of the pipette head module 2 is that the upper end of a first baffle 2.15 contacts and coincides with the upper end of a first initial positioning block 2.6, the initial position of the upper liquid module 3 is that a stop block arranged on the upper part of a bottle seat 3.17 contacts with the upper end of a second initial positioning block 3.8, the initial position of the sample injection module 4 is that the stop block on the upper part of a second test tube rack 4.8 contacts with the upper end of a third initial positioning block 4.7, the initial position of the pipette head module 1 is that a photosensitive center of a photoelectric sensor 1.5 aligns with one of the photoelectric sensor detection plates 1.6, the initial position of the pipette head module 5 is that a seventh slide block 5.8 moves to the upper end surface of a sixth top plate 5.9, and the initial position of the pipette head module is that a fifth slide block 6.9 moves to the upper end.
S2: barrel loading: the gun head module 2 is lifted up to enable the gun barrel 2.8 of the gun head disc 2.11 to be sleeved on the pipette, then the gun head module 2 is lifted down, and the descending distance is ensured to be larger than the length of the gun barrel 2.8;
s3: the rotating shaft 1.13 rotates by 90 degrees, so that the first liquid transfer gun 1.1 rotates to be above the upper liquid module 3, the fourth liquid transfer gun 1.4 rotates to be above the gun head entering module 2, the gun head entering module 2 rotates the gun head disc 2.11 to enable the gun barrel 2.8 to face the fourth liquid transfer gun 1.4 and ascend, and the fourth liquid transfer gun 1.4 finishes the operation of the gun barrel 2.8 in the step S2;
s4: liquid feeding operation: the upper liquid module 3 ascends, a first reagent bottle 3.1 is immersed under a barrel 2.8 of a first liquid-transferring gun 1.1, the first liquid-absorbing and discharging module 6 drives a top rod 6.4 to descend, a piston of the first liquid-transferring gun 1.1 is compressed to a first position Q1, then the top rod 6.4 ascends, and at the moment, the first liquid-transferring gun 1.1 absorbs the magnetic bead solution with the required measuring range;
s5: the rotating shaft 1.13 rotates by 90 degrees, so that the first liquid transfer gun 1.1 rotates to the position above the sample injection module 4, the fourth liquid transfer gun 1.4 rotates to the position above the upper liquid module 3, the third liquid transfer gun 1.3 rotates to the position above the gun head entering module 2, the gun head entering module 2 rotates a gun head disc 2.11, so that a gun barrel 2.8 is over against the third liquid transfer gun 1.3 and ascends, the third liquid transfer gun 1.3 completes S2 gun tube loading operation, and at the moment, the fourth liquid transfer gun 1.4 does not absorb any liquid;
s6: the second liquid suction and discharge module 7 compresses the piston of the first liquid transfer gun 1.1 through the mandril 6.4, so that the magnetic bead solution in the first liquid transfer gun 1.1 is output to the open test tube 4.12;
s7: rotating the rotating shaft 1.13 by 90 degrees, so that the first pipetting gun 1.1 rotates to the position below the gun head withdrawing module 5, the fourth pipetting gun 1.4 rotates to the position above the open test tube 4.12, the third pipetting gun 1.3 rotates to the position above the upper liquid module 3, the first rotary disc 3.14 of the upper liquid module 3 rotates by 90 degrees, the second reagent bottle 3.2 is aligned with the third pipetting gun 1.3, the liquid operation in the step S4 is completed, and at this time, the third pipetting gun 1.3 absorbs the PBS-T solution with the required measuring range; the sample introduction module 4 ascends to enable the gun barrel 2.8 of the fourth pipette 1.4 to contact the bottom of the open test tube 4.12, the ejector rod 6.4 of the second pipette module 7 is controlled to ascend and descend continuously, so that liquid in the fourth pipette 1.4 is sucked and output continuously, and the magnetic bead solution and the chicken extracting solution in the open test tube 4.12 are mixed uniformly; after uniform mixing, the sample injection module 4 is lowered to the initial position; an execution plate 5.3 of the gun head withdrawing module 5 moves downwards to push a push plate of the first liquid transferring gun 1.1 to move, so that a gun barrel 2.8 is driven to fall into a collecting tank 8 below;
s8: magnetic separation operation: rotating a second rotating disc 4.4 of the sample introduction module 4, rotating the open test tube rack 4.5 to a magnet fixing frame 9.5 aligned with the magnetic separation module 9, controlling two magnet fixing frames 9.5 of the magnetic separation module 9 to move forwards to two sides of the outer wall of the open test tube 4.12, after standing, collecting magnetic beads in the open test tube 4.12 on the wall of the test tube, moving the magnet fixing frame 9.5 backwards, and rotating the second rotating disc 4.4 of the sample introduction module 4 for 90 degrees to an initial position;
s9: controlling the sample introduction module 4 to ascend, so that a barrel 2.8 of a fourth pipette 1.4 contacts the bottom of an open test tube 4.12, controlling a mandril 6.4 of a second liquid suction and discharge module 7 to descend and ascend, and sucking the separated clear liquid in the open test tube 4.12 into the barrel 2.8 of the fourth pipette 1.4;
s10: the rotating shaft 1.13 rotates by 90 degrees, the third liquid transferring gun 1.3 is transferred to the upper part of the sample feeding module 4, the fourth liquid transferring gun 1.1 is transferred to the lower part of the gun head withdrawing module 5, and the second liquid transferring gun 1.2 is transferred to the upper part of the upper liquid module 3; the first liquid transfer gun 1.1 is transferred to the position above the gun head entering module 2, the gun tube loading operation in the step S2 is completed, and at the moment, the second liquid transfer gun 1.2 does not absorb any liquid; the second liquid suction and discharge module 7 drives the ejector rod 6.4 to descend, and a piston of the third liquid transfer gun 1.3 is compressed to the second position, so that PBS-T solution in the third liquid transfer gun 1.3 is discharged to the open test tube 4.12; an execution plate 5.3 of the gun head withdrawing module 5 moves downwards to push a push plate of a fourth liquid transferring gun 1.4, so that a gun barrel 2.8 falls into a collecting tank 8 below;
s11: the rotating shaft 1.13 rotates for 90 degrees, so that the third liquid transferring gun 1.3 is transferred to the position below the gun head withdrawing module 5, and the second liquid transferring gun 1.2 is transferred to the position above the sample feeding module 4; the fourth liquid transferring gun 1.4 is transferred to the upper part of the gun head entering module 2, and the first liquid transferring gun 1.1 is transferred to the upper part of the upper liquid module 3; the first pipetting gun 1.1 does not absorb any liquid at this point; the sample introduction module 4 ascends to enable a gun barrel 2.8 of the second pipette 1.2 to contact the bottom of the open test tube 4.12, and liquid in the open test tube 4.12 is continuously sucked and discharged by controlling the mandril 6.4 of the second pipette module 7 to continuously ascend and descend, so that the PBS-T solution and the magnetic beads after magnetic separation are uniformly mixed; an execution plate 5.3 of the gun head withdrawing module 5 moves downwards to push a push plate of a third liquid transferring gun 1.3, so that a gun barrel 2.8 falls into a collecting tank 8 below; the gun head entering module 2 rotates and raises the gun head disc 2.11, so that the fourth liquid-transfering gun 1.4 finishes the gun tube loading operation of the step S2;
s12: after the liquid in the open test tube 4.12 is uniformly mixed, performing the magnetic separation operation of step S8;
s13: the rotating shaft 1.13 rotates by 90 degrees, so that the first liquid transferring gun 1.1 is transferred to the position above the sample feeding module 4, the fourth liquid transferring gun 1.4 is transferred to the position above the upper liquid module 3, the third liquid transferring gun 1.3 is transferred to the position above the gun head entering module 2, and the second liquid transferring gun 1.2 is transferred to the position below the gun head withdrawing module 5;
controlling the sample introduction module 4 to ascend, so that the barrel 2.8 of the first pipette 1.1 is in contact with the bottom of the open test tube 4.12, compressing the first pipette 1.1 by controlling the ejector rod 6.4 of the second pipette module 7, and then ascending, so that separated clear liquid is sucked into the barrel 2.8 of the first pipette 1.1; an execution plate 5.3 of the gun head withdrawing module 5 moves downwards to push a push plate of a second liquid transferring gun 1.2 to move, so that a gun barrel 2.8 falls into a collecting tank 8 below; the gun head entering module 2 rotates the gun head disc 2.11 to enable the third liquid-transfering gun 1.3 to face the unused gun barrel 2.8, and raises the gun head disc 2.11 to enable the third liquid-transfering gun 1.3 to complete the gun tube loading operation of the step S2;
the first rotary disc 3.14 of the upper liquid module 3 rotates 90 degrees, so that the gun tube 2.8 of the fourth liquid-transferring gun 1.4 is aligned with the reagent bottle 3.3 No. three, the first liquid-absorbing and discharging module 6 drives the first ejector rod 6.4 to descend, the piston of the fourth liquid-transferring gun 1.4 is compressed to a first position Q1, then the ejector rod 6.4 ascends, and at the moment, the fourth liquid-transferring gun 1.4 absorbs PBS-BSA liquid with a required measuring range;
s14: the rotating shaft 1.13 rotates by 90 degrees, so that the first liquid transferring gun 1.1 is transferred to the position below the gun head withdrawing module 5, the second liquid transferring gun 1.2 is transferred to the position above the gun head entering module 2, the third liquid transferring gun 1.3 is transferred to the position above the upper liquid module 3, and the fourth liquid transferring gun 1.4 is transferred to the position above the sample feeding module 4;
an execution plate 5.3 of the gun head withdrawing module 5 moves downwards to push a push plate of the first liquid transferring gun 1.1 to move downwards, so that a gun barrel 2.8 is pushed to fall into a collecting tank 8 below; the gun head entering module 2 rotates the gun head disc 2.11 to enable the second liquid-transferring gun 1.2 to face the unused gun barrel 2.8, and raises the gun head disc 2.11 to enable the second liquid-transferring gun 1.2 to complete the gun tube loading operation of the step S2; the sample introduction module 4 ascends to enable the gun tube 2.8 of the fourth pipette 1.4 to contact the bottom of the open test tube 4.12, the ejector rod 6.4 of the second pipette module 7 is controlled to ascend and descend continuously, so that liquid in the open test tube 4.12 is sucked and discharged continuously, the PBS-BSA solution in the open test tube 4.12 and the solution after magnetic separation in the step S13 are mixed uniformly, and the sample introduction module 4 descends to an initial position;
the first rotating disc 3.14 of the upper liquid module 3 rotates by 90 degrees, so that the reagent bottle No. four 3.4 is aligned with the third liquid transferring gun 1.3, the first liquid sucking and discharging module 6 drives the ejector rod 6.4 to descend, the piston of the third liquid transferring gun 1.3 is compressed to a first position Q1, then the ejector rod 6.4 ascends, and at the moment, the third liquid transferring gun 1.3 absorbs the quantum dot solution with the required measuring range;
s15: the rotating shaft 1.13 rotates by 90 degrees, the first liquid transferring gun 1.1 is transferred to the position above the gun head entering module 2, the second liquid transferring gun 1.2 is transferred to the position above the upper liquid module 3, the third liquid transferring gun 1.3 is transferred to the position above the sample introduction module 4, and the fourth liquid transferring gun 1.4 is transferred to the position below the gun head withdrawing module 5;
the gun head feeding module 2 rotates the gun head disc 2.11 to enable the first liquid-transferring gun 1.1 to face the unused gun barrel 2.8, and raises the gun head disc 2.11 to enable the first liquid-transferring gun 1.1 to complete the gun tube loading operation of the step S2; an execution plate 5.3 of the gun head withdrawing module 5 moves downwards to push a push plate of a fourth liquid transferring gun 1.4 to move, so that a gun barrel 2.8 below the push plate is pushed to fall into a collecting tank 8;
and controlling the sample injection module 4 to ascend to enable the barrel 2.8 of the third pipette 1.3 to contact the bottom of the open test tube 4.12, controlling the ejector rod 6.4 of the second pipette module 7 to ascend and descend continuously to enable liquid in the open test tube 4.12 to be sucked and discharged continuously, so that the quantum dot solution, the PBS-BSA solution and the solution after the magnetic separation in the step S13 in the open test tube 4.12 are mixed uniformly, and the sample injection module 4 descends to an initial position.
S16: after the liquids in the open test tube 4.12 are mixed uniformly, the magnetic separation operation of step S8 is performed.
S17: the rotating shaft 1.13 rotates by 90 degrees, so that the first liquid transferring gun 1.1 is transferred to the upper part of the upper liquid module 3, the second liquid transferring gun 1.2 is transferred to the sample introduction module 4, the third liquid transferring gun 1.3 is transferred to the lower part of the gun head withdrawing module 5, and the fourth liquid transferring gun 1.4 is transferred to the upper part of the gun head entering module 2.
Controlling the sample introduction module 4 to ascend, so that a barrel 2.8 of the second pipette 1.2 contacts the bottom of the open test tube 4.12, the first pipette module 6 drives the ejector rod 6.4 to descend, a piston of the second pipette is compressed to a first position Q1, and then the ejector rod 6.4 ascends, so that separated clear liquid is sucked into the second pipette 1.2;
an execution plate 5.3 of the gun head withdrawing module 5 moves downwards to push a push plate of a third liquid transferring gun 1.3, so that a gun barrel 2.8 below the push plate is pushed to fall into a collecting tank 8; the gun head entering module 2 rotates the gun head disc 2.11 to enable the fourth liquid-transferring gun 1.4 to face the unused gun barrel 2.8, and raises the gun head disc 2.11 to enable the fourth liquid-transferring gun 1.4 to complete the gun tube loading operation of the step S2;
the third reagent bottle 3.3 is aligned to the first liquid transferring gun 1.1 by rotating the first rotary disc 3.14 of the upper liquid module 3, the first liquid absorbing and discharging module 6 downwards compresses the piston of the first liquid transferring gun 1.1 through the ejector rod 6.4, the ejector rod 6.4 ascends, and the first liquid transferring gun 1.1 absorbs PBS-BSA liquid with the required measuring range;
s18: the rotating shaft 1.13 rotates by 90 degrees, so that the second liquid transferring gun 1.2 is transferred to the position below the gun head withdrawing module 5, the first liquid transferring gun 1.1 is transferred to the position above the sample feeding module 4, the third liquid transferring gun 1.3 is transferred to the position above the gun head feeding module 2, and the fourth liquid transferring gun 1.4 is transferred to the position above the upper liquid module 3.
The sample introduction module 4 ascends to enable the gun barrel 2.8 of the first liquid transfer gun 1.1 to contact the bottom of the open test tube 4.12, the ejector rod 6.4 of the second liquid suction and discharge module 7 is controlled to ascend and descend continuously, liquid in the open test tube 4.12 is sucked and discharged continuously, the PBS-BSA solution and the solution after magnetic separation in the step S18 are mixed uniformly, and the sample introduction module 4 descends to an initial position;
an execution plate 5.3 of the gun head withdrawing module 5 moves downwards, and a gun barrel 2.8 connected with a push plate is dropped into a collection tank 8 by pushing the push plate of the second pipetting gun 1.2;
s19: rotating the rotating shaft by 1.13 degrees to 90 degrees, so that the first liquid transferring gun 1.1 is transferred to the position below the gun head withdrawing module, the second liquid transferring gun 1.2 is transferred to the position above the gun head entering module 2, the third liquid transferring gun 1.3 is transferred to the position above the upper liquid module 3, and the fourth liquid transferring gun 1.4 is transferred to the position above the sample feeding module; the sample introduction module 4 ascends to enable the gun barrel 2.8 of the fourth pipette 1.4 to contact the bottom of the open test tube 4.12, the ejector rod 6.4 of the second pipette module 7 is controlled to descend, the piston of the fourth pipette 1.4 is compressed to a first position Q1, then the ejector rod 6.4 ascends, and at the moment, the fourth pipette 1.4 absorbs the liquid in the open test tube 4.12 with the required measuring range;
the second rotating disc 4.4 of the sample introduction module 4 rotates 180 degrees, so that the second test tube rack 4.8 is aligned to the fourth pipetting gun 1.4, then the second liquid suction and discharge module 7 compresses the mandril 6.4 to the second position Q2 of the fourth pipetting gun 1.4, and liquid in the pipetting gun is discharged;
a second rotating disc 4.4 of the sample introduction module 4 rotates 180 degrees, and a second test tube 4.6 is taken out for subsequent detection of the microplate reader/spectrometer;
s20: rotating the rotating shaft by 1.13 for 90 degrees to enable the fourth liquid gun 1.4 to be rotated to the position below the gun head withdrawing module 5, the third liquid transferring gun 1.3 to the position above the sample feeding module 4, the second liquid transferring gun 1.2 to the position above the upper liquid module 3, and the first liquid transferring gun 1.1 to the position above the gun head feeding module 2;
s21: and resetting each module to the initial position, and ending.
Claims (6)
1. A pretreatment device for chicken extract is characterized by comprising a frame, a liquid-transfering gun module (1), a gun head feeding module (2), an upper liquid module (3), a sample feeding module (4) and a gun head withdrawing module (5), the liquid suction and drainage device comprises a first liquid suction and drainage module (6), a second liquid suction and drainage module (7), a collecting tank (8) and a magnetic separation module (9), wherein a liquid transfer gun module (1) is installed in the middle of a rack, a liquid inlet gun head module (2), an upper liquid module (3), a sample introduction module (4) and the collecting tank (8) are installed on the periphery of the liquid transfer gun module (1) and positioned at the bottom of the rack through the rack respectively, the liquid inlet gun head module (2) and the sample introduction module (4) are arranged oppositely, the upper liquid module (3) and the collecting tank (8) are arranged oppositely, and the magnetic separation module (9) is installed on one side, close to the upper liquid module (3), of the sample; a first liquid suction and discharge module (6) is arranged above the upper liquid module (3) and positioned at the top of the rack, a second liquid suction and discharge module (7) is arranged above the sample injection module (4) and positioned at the top of the rack, and a gun head withdrawing module (5) is arranged at the top of the rack;
the liquid-transfering gun module (1) comprises a liquid-transfering gun, a liquid-transfering gun seat (1.14), a liquid-transfering gun tray (1.12), a liquid-transfering gun module bottom plate (1.9), a motor connecting plate (1.15), an angular contact ball bearing seat (1.11), a fourth rotating motor (1.10), a rotating shaft (1.13), a photoelectric sensor (1.5) and a photoelectric sensor detection plate (1.6); a bottom plate (1.9) of a liquid-transferring gun module is fixed at the center of the bottom of the rack, a motor connecting plate (1.15) is fixed above the bottom plate (1.9) of the liquid-transferring gun module through two supporting plates (1.8), a fourth rotating motor (1.10) is installed on the lower surface of the motor connecting plate (1.15), and an output shaft of the fourth rotating motor (1.10) penetrates out of the motor connecting plate (1.15) upwards and is connected with a coupler (1.16); an angular contact ball bearing seat (1.11) is fixed above the upper surface of a motor connecting plate (1.15) through two copper columns (1.7), a rotating shaft (1.13) is installed in the middle of the angular contact ball bearing seat (1.11) through an angular contact ball bearing (1.17), a liquid-transferring gun plate (1.12) is installed at the upper end of the rotating shaft (1.13), the lower end of the rotating shaft (1.13) is connected with an output shaft of a fourth rotating motor (1.10) through a coupler (1.16), and a photoelectric sensor (1.5) is installed on one side, close to the copper columns (1.7), of the upper surface of the angular contact ball bearing seat (1.11) through a connecting plate; the upper end surface of the liquid transfer gun tray (1.12) is circumferentially and uniformly provided with four liquid transfer gun seats (1.14) close to the edge, each liquid transfer gun seat (1.14) is provided with a liquid transfer gun with the lower end extending out of the liquid transfer gun tray (1.12), the lower end surface of the liquid transfer gun tray (1.12) corresponding to each liquid transfer gun seat (1.14) is fixedly provided with a photoelectric sensor detection plate (1.6), and the photoelectric sensor detection plate (1.6) is used for positioning the liquid transfer gun seat (1.14) after being contacted with a photoelectric sensor (1.5) positioned below the photoelectric sensor detection plate; the liquid-transfering gun plate (1.12) rotates around the center of the rotating shaft (1.13) under the drive of the fourth rotating motor (1.10);
the gun head feeding module (2) comprises a first moving motor (2.1), a first top plate (2.7), a first bottom plate (2.2), a first connecting plate (2.5), a first sliding block (2.3), a first optical axis (2.4), a first inverted L-shaped connecting plate (2.13), a gun barrel (2.8), a first rotating motor (2.10), a gun head feeding module connecting plate (2.12), a gun head disc (2.11) and a gun disc seat (2.14); the liquid transferring gun comprises a liquid transferring gun module (1), a liquid transferring gun module (2), a first connecting plate (2.5), a first top plate (2.7), a first bottom plate (2.2), a first optical axis (2.4), a first initial positioning block (2.6), a first moving motor (2.1), a first inverted-L-shaped connecting plate (2.13), a first connecting plate (2.2), a second connecting plate (2.12), a first rotating motor (2.10) is installed on the lower end face of a horizontal plate of a first inverted L-shaped connecting plate (2.13), an output shaft of the first rotating motor (2.10) upwards sequentially penetrates through the horizontal plane of the first inverted L-shaped connecting plate (2.13) and a first flange (2.9) and then is connected with a gun plate seat (2.14), a gun head plate (2.11) is fixed on the upper end face of the gun plate seat (2.14), a plurality of through holes are circumferentially and uniformly distributed on the end face of the gun head plate (2.11) along the edge and used for placing gun tubes (2.8), a first baffle plate (2.15) is fixed on the upper end face of the gun head plate (2.11) and positioned between the two through holes, and the upper end of the first baffle plate (2.15) is used for contacting with the upper end of a first initial positioning block (2.6) to realize initial positioning of the gun head plate (2.; the first sliding block (2.3) is driven by the first moving motor (2.1) to move up and down along the first optical axis (2.4), so that a gun head disc (2.11) connected with the first inverted L-shaped connecting plate (2.13) is driven to move up and down, and the gun head disc (2.11) is driven by the first rotating motor (2.10) to rotate around a central shaft;
the upper liquid module (3) comprises a second moving motor (3.11), a second top plate (3.16), a second initial positioning block (3.8), a second connecting plate (3.15), a second sliding block (3.10), a second optical axis (3.9), a second inverted L-shaped connecting plate (3.6), a second rotating motor (3.5), a first rotating disc (3.14), an upper liquid module connecting plate (3.12) and a bottle seat (3.17); the upper liquid module (3) is fixed on the frame through an upper liquid module connecting plate (3.12) which is vertically arranged, the side surface of a second connecting plate (3.15) is attached to the side surface of the upper liquid module connecting plate (3.12) facing the liquid-moving gun module (1), a second top plate (3.16) and a second bottom plate (3.13) are respectively installed on the upper end surface and the lower end surface of the second connecting plate (3.15), a second optical axis (3.9) is connected between the second top plate (3.16) and the second bottom plate (3.13), a second initial positioning block (3.8) is installed on the upper end surface of the second top plate (3.16), a second moving motor (3.11) is installed on the lower end surface of the second bottom plate (3.13), the output shaft of the second moving motor (3.11) penetrates through the second bottom plate (3.13) upwards to be connected with a second sliding block (3.10) sleeved on the second optical axis (3.9), and the vertical connecting plate of a second inverted L-shaped connecting plate (3.6) is connected with the side surface of the second sliding block (3.10) far away from the second connecting, a second rotating motor (3.5) is installed on the lower end face of a horizontal plate of the second inverted L-shaped connecting plate (3.6), an output shaft of the second rotating motor (3.5) upwards sequentially penetrates through the horizontal plane of the second inverted L-shaped connecting plate (3.6) and the second flange (3.7) and then is connected with the first rotary table (3.14), four bottle seats (3.17) which are symmetrically arranged in pairs are fixed on the upper end face of the first rotary table (3.14), and reagent bottles are installed in each bottle seat (3.17); a stop block is arranged at the upper part of each bottle seat (3.17) and is used for contacting the upper end of the second initial positioning block (3.8) to realize the initial positioning of the first rotary table (3.14); the second sliding block (3.10) is driven by a second moving motor (3.11) to move up and down along a second optical axis (3.9), so that a first rotating disc (3.14) of the second inverted-L-shaped connecting plate (3.6) is driven to move up and down, and the first rotating disc (3.14) is driven by a second rotating motor (3.5) to rotate around a central shaft;
the sample introduction module (4) comprises a third moving motor (4.1), a third top plate (4.16), a third bottom plate (4.15), a third connecting plate (4.10), a third sliding block (4.11), a third optical axis (4.9), a third inverted L-shaped connecting plate (4.14), a third rotating motor (4.2), a third flange (4.3), a sample introduction module connecting plate (4.13), a second rotating disc (4.4), an open test tube (4.12), an open test tube rack (4.5), a second test tube (4.6), a second test tube rack (4.8) and a third initial positioning block (4.7); the sample introduction module (4) is fixed on the frame through a sample introduction module connecting plate (4.13) which is vertically arranged, the side surface of a third connecting plate (4.10) is attached to the side surface of the sample introduction module connecting plate (4.13) facing the liquid transfer gun module (1), the upper end surface and the lower end surface of the third connecting plate (4.10) are respectively provided with a third top plate (4.16) and a third bottom plate (4.15), a third optical axis (4.9) is connected between the third top plate (4.16) and the third bottom plate (4.15), the upper end surface of the third top plate (4.16) is provided with a third initial positioning block (4.7), the lower end surface of the third bottom plate (4.15) is provided with a third moving motor (4.1), the output shaft of the third moving motor (4.1) penetrates through the third bottom plate (4.15) upwards to be connected with a third sliding block (4.11) sleeved on the third optical axis (4.9), the vertical plate of the third inverted L-shaped connecting plate (4.14) is connected with the side surface of the third sliding block (4.11), a third rotating motor (4.2) is installed on the lower end face of a horizontal plate of a third inverted L-shaped connecting plate (4.14), an output shaft of the third rotating motor (4.2) upwards sequentially penetrates through the horizontal plane of the third inverted L-shaped connecting plate (4.14), a third flange (4.3) is connected with a second rotary table (4.4), a second test tube rack (4.8) is fixed on one side of the upper end face of the second rotary table (4.4), a second test tube (4.6) is placed in the middle of the second test tube rack (4.8), a stop block is arranged on one side, far away from the opening test tube rack (4.5), of the upper portion of the second test tube rack (4.8) and is used for contacting with the upper end of a third initial positioning block (4.7) to achieve initial positioning of the second rotary table (4.4); an open test tube rack (4.5) is mounted on the other side of the upper end face of the second rotary table (4.4), a cylindrical through hole for placing an open test tube (4.12) is formed in the upper portion of the open test tube rack (4.5), a through groove penetrating through the front portion and the rear portion is formed in the lower portion of the open test tube rack (4.5), and the lower end of the open test tube (4.12) extends out of the through hole and is placed in the through groove; the third sliding block (4.11) is driven by a third moving motor (4.1) to move up and down along a third optical axis (4.9), so that a second rotating disc (4.4) of a third inverted L-shaped connecting plate (4.14) is driven to move up and down, and the second rotating disc (4.4) is driven by a third rotating motor (4.2) to rotate around a central shaft;
the magnetic separation module (9) comprises a fourth moving motor (9.1), a front plate (9.2), a rear plate (9.4), a fourth connecting plate (9.3), a fourth sliding block (9.7), a fourth optical axis (9.8), a fourth L-shaped connecting plate (9.6), a magnet (9.9) and a magnet fixing frame (9.5); the magnetic separation module (9) is fixed on the rack through a fourth connecting plate (9.3) which is horizontally arranged, a front plate (9.2) and a rear plate (9.4) are respectively fixed at the front end and the rear end of the fourth connecting plate (9.3), a fourth optical axis (9.8) is connected between the front plate (9.2) and the rear plate (9.4), a fourth moving motor (9.1) is installed at one side, far away from the liquid-transfering gun module (1), of the front plate (9.2), an output shaft of the fourth moving motor (9.1) sequentially penetrates through the front plate (9.2) to be connected with a fourth sliding block (9.7), the lower end face of a horizontal plate of the fourth L-shaped connecting plate (9.6) is connected with the upper end face of the fourth sliding block (9.7), two magnet fixing frames (9.5) which are arranged in a V shape are fixed on the side face, close to the liquid-transfering gun module (1), of a vertical plate of the fourth L-shaped connecting plate (9.6), and magnets (9.5) are nested in a magnet sleeve (9.; the fourth sliding block (9.7) slides along a fourth optical axis (9.8) under the driving of a fourth moving motor (9.1), so that a magnet fixing frame (9.5) connected with a fourth L-shaped connecting plate (9.6) is driven to move towards a through groove of the open test tube rack (4.5), and magnets (9.9) nested at the front ends of the two magnet fixing frames (9.5) move to two sides of the outer wall of an open test tube (4.12) in the through groove;
the first liquid suction and discharge module (6) and the second liquid suction and discharge module (7) are liquid suction and discharge modules, and each liquid suction and discharge module comprises a fifth moving motor (6.1), a fifth top plate (6.2), a fifth bottom plate (6.6), a fifth connecting plate (6.7), a liquid suction and discharge module connecting plate (6.8), a fifth sliding block (6.9), a fifth optical axis (6.5), a fifth L-shaped connecting plate (6.3) and an ejector rod (6.4); the liquid suction and discharge module is fixed on the frame through a vertically arranged liquid suction and discharge module connecting plate (6.8), the side surface of a fifth connecting plate (6.7) is jointed with the side surface of the liquid suction and discharge module connecting plate (6.8) facing the liquid transfer gun module (1), on the fifth connecting plate (6.7), a fifth top plate (6.2) and a fifth bottom plate (6.6) are respectively installed on the lower end face of the upper end face of the fifth L-shaped connecting plate, a fifth optical axis (6.5) is connected between the fifth top plate (6.2) and the fifth bottom plate (6.6), a fifth moving motor (6.1) is installed on the upper end face of the fifth top plate (6.2), an output shaft of the fifth moving motor (6.1) penetrates out of the fifth top plate (6.2) downwards to be connected with a fifth sliding block (6.9) sleeved on the fifth optical axis (6.5), a vertical plate of the fifth L-shaped connecting plate (6.3) is connected with the side face, close to the liquid-transferring gun module (1), of the fifth sliding block (6.9), and an ejector rod (6.4) is installed on the lower end face of a horizontal plate of the fifth L-shaped connecting plate; the fifth sliding block (6.9) is driven by a fifth moving motor (6.1) to slide along a fifth optical axis (6.5), so that a mandril (6.4) connected with the fifth L-shaped connecting plate (6.3) is driven to move downwards to push the top end of the liquid-transferring gun for sucking or outputting liquid;
the gun head withdrawing module (5) comprises a sixth moving motor (5.1), a sixth top plate (5.9), a sixth bottom plate (5.5), a seventh sliding block (5.8), a sixth optical axis (5.4), a sixth connecting plate (5.6), a seventh connecting plate (5.2), a gun head withdrawing connecting plate (5.7) and an execution plate (5.3); the gun head withdrawing module (5) is fixed on the rack through a vertically arranged gun head withdrawing connecting plate (5.7), the side face of a sixth connecting plate (5.6) is attached to the side face, facing the liquid transferring gun module (1), of the gun head withdrawing connecting plate (5.7), the upper end face and the lower end face of the sixth connecting plate (5.6) are respectively provided with a sixth top plate (5.9) and a sixth bottom plate (5.5), a sixth optical axis (5.4) is connected between the sixth top plate (5.9) and the sixth bottom plate (5.5), the upper end face of the sixth top plate (5.9) is provided with a sixth moving motor (5.1), an output shaft of the sixth moving motor (5.1) penetrates out of the sixth top plate (5.9) downwards to be connected with a seventh sliding block (5.8) sleeved on the sixth optical axis (5.4), and the execution plate (5.3) is connected with the side face, close to the liquid transferring gun module (1), of the seventh sliding block (5.8), through a seventh connecting plate (5.2); the seventh sliding block (5.8) slides along a sixth optical axis (5.4) under the driving of a sixth moving motor (5.1), so that an execution plate (5.3) connected with the seventh connecting plate (5.2) is driven to move downwards to push a push plate of the liquid-transfering gun, and a gun barrel (2.8) connected with the push plate is pushed to drop into a collecting tank (8) below.
2. The pretreatment device for chicken extract according to claim 1, wherein the four pipette guns are respectively a first pipette gun (1.1), a second pipette gun (1.2), a third pipette gun (1.3) and a fourth pipette gun (1.4), one of the gun barrels (2.8) of the head module (2) is aligned below the first pipette gun (1.1), one of the bottle holders (3.17) of the upper liquid module (3) is aligned below the second pipette gun (1.2), an open test tube (4.12) of the sample injection module (4) is aligned below the third pipette gun (1.3), and a collection tank (8) is aligned below the fourth pipette gun (1.4).
3. The pretreatment device for chicken extracting solution according to claim 1, wherein the center of the top rod (6.4) of the first liquid suction and discharge module (6) is located right above the second liquid transfer gun (1.2), and the center of the top rod (6.4) of the second liquid suction and discharge module (7) is located right above the third liquid transfer gun (1.3); the center of an execution plate (5.3) of the gun head withdrawing module (5) is aligned with a push plate of a fourth pipetting gun (1.4) right below.
4. The pretreatment device for chicken extracting solution according to claim 1, wherein the end faces of two poles of the magnet (9.9) opposite to each other are isolated from the outside by a magnetic sleeve, so as to prevent strong attraction force generated by the mutual pairing of N-S poles; the left and right end faces of the magnet (9.9) are not isolated from the outside through the magnetic sleeves.
5. A pretreatment method for chicken extract by using the device of any one of claims 1 to 4, characterized by comprising the following steps
S1: a gun barrel (2.8) is placed on a gun head disc (2.11), a first reagent bottle to a fourth reagent bottle are respectively placed in four bottle seats (3.17), a magnetic bead solution is filled in the first reagent bottle (3.1), a PBS-T solution is filled in the second reagent bottle (3.2), a PBS-BSA solution is filled in the third reagent bottle (3.3), and a quantum dot solution is filled in the fourth reagent bottle (3.4); putting the chicken extract solution into an open test tube (4.12) in advance, putting the open test tube (4.12) into an open test tube rack (4.5), putting a second test tube (4.6) into a second test tube rack (4.8), returning each module to the initial position, and adjusting the range required by the liquid-transfering gun;
s2: loading a gun barrel; the gun head module (2) is lifted to enable the gun barrel (2.8) of the gun head disc (2.11) to be sleeved on the liquid transferring gun, and then the gun head module (2) is lifted to a lower position, wherein the descending distance is ensured to be larger than the length of the gun barrel (2.8);
s3: the rotating shaft (1.13) rotates by 90 degrees, so that the first liquid transfer gun (1.1) rotates to the upper part of the upper liquid module (3), the fourth liquid transfer gun (1.4) rotates to the upper part of the gun head entering module (2), the gun head entering module (2) rotates the gun head disc (2.11) to enable the gun barrel (2.8) to face the fourth liquid transfer gun (1.4) and ascend, and the fourth liquid transfer gun (1.4) completes the operation of assembling the gun barrel (2.8) in the step S2;
s4: liquid feeding operation: the upper liquid module (3) ascends, a first reagent bottle (3.1) is immersed under a barrel (2.8) of a first liquid transferring gun (1.1), the first liquid absorbing and discharging module (6) presses a piston of the first liquid transferring gun (1.1) downwards through a top rod (6.4), then the top rod (6.4) ascends, and at the moment, the first liquid transferring gun (1.1) absorbs the magnetic bead solution with the required measuring range;
s5: the rotating shaft (1.13) rotates by 90 degrees, so that the first liquid transferring gun (1.1) rotates to the position above the sample feeding module (4), the fourth liquid transferring gun (1.4) rotates to the position above the upper liquid module (3), the third liquid transferring gun (1.3) rotates to the position above the liquid feeding head module (2), the liquid feeding head module (2) rotates the gun head disc (2.11) to enable the gun barrel (2.8) to face the third liquid transferring gun (1.3) and ascend, the third liquid transferring gun (1.3) completes S2 gun tube loading operation, and at the moment, the fourth liquid transferring gun (1.4) does not absorb any liquid;
s6: the second liquid suction and discharge module (7) compresses the piston of the first liquid transferring gun (1.1) through the ejector rod (6.4), so that the magnetic bead solution in the first liquid transferring gun (1.1) is output to the open test tube (4.12);
s7: rotating the rotating shaft (1.13) by 90 degrees to enable the first liquid transferring gun (1.1) to rotate to the position below the gun head withdrawing module (5), enabling the fourth liquid transferring gun (1.4) to rotate to the position above the open test tube (4.12), enabling the third liquid transferring gun (1.3) to rotate to the position above the upper liquid module (3), enabling the first rotary disc (3.14) of the upper liquid module (3) to rotate by 90 degrees, aligning the second reagent bottle (3.2) with the third liquid transferring gun (1.3), and completing the liquid operation in the step S4, wherein the third liquid transferring gun (1.3) absorbs PBS-T solution with the required range; the sample introduction module (4) ascends to enable a gun barrel (2.8) of the fourth liquid transfer gun (1.4) to contact the bottom of the open test tube (4.12), and a mandril (6.4) of the second liquid suction and discharge module (7) is controlled to continuously ascend and descend to enable liquid in the fourth liquid transfer gun (1.4) to be continuously sucked and output, so that the magnetic bead solution and the chicken extracting solution in the open test tube (4.12) are uniformly mixed; after uniform mixing, the sample injection module (4) descends to an initial position; an execution plate (5.3) of the gun head withdrawing module (5) moves downwards to push a push plate of the first liquid transferring gun (1.1) to move, and a gun barrel (2.8) is driven to fall into a collecting tank (8) below;
s8: magnetic separation operation, namely rotating a second rotating disc (4.4) of the sample injection module (4), rotating the open test tube rack (4.5) to a magnet fixing frame (9.5) aligned with the magnetic separation module (9), controlling two magnet fixing frames (9.5) of the magnetic separation module (9) to move forwards to two sides of the outer wall of the open test tube (4.12), standing, enabling magnetic beads in the open test tube (4.12) to be enriched on the wall of the test tube, moving the magnet fixing frame (9.5) backwards, and rotating the second rotating disc (4.4) of the sample injection module (4) for 90 degrees to an initial position;
s9: controlling the sample introduction module (4) to ascend, enabling a gun barrel (2.8) of the fourth pipette (1.4) to contact the bottom of the open test tube (4.12), controlling a mandril (6.4) of the second liquid suction and discharge module (7) to descend and then ascend, and sucking the separated clear liquid in the open test tube (4.12) into the gun barrel (2.8) of the fourth pipette (1.4);
s10: the rotating shaft (1.13) rotates by 90 degrees, the third liquid transferring gun (1.3) is transferred to the upper part of the sample introduction module (4), the fourth liquid transferring gun (1.1) is transferred to the lower part of the gun head withdrawing module (5), and the second liquid transferring gun (1.2) is transferred to the upper part of the upper liquid module (3); the first liquid transferring gun (1.1) is transferred to the position above the gun head entering module (2) to finish the gun tube loading operation of the step S2, and the second liquid transferring gun (1.2) does not absorb any liquid; the second liquid suction and discharge module (7) compresses a piston of the third liquid transferring gun (1.3) through a mandril (7.4) so that the PBS-T solution in the third liquid transferring gun (1.3) is discharged to an open test tube (4.12); an execution plate (5.3) of the gun head withdrawing module (5) moves downwards to push a push plate of a fourth liquid transferring gun (1.4), so that a gun barrel (2.8) falls into a collecting tank (8) below;
s11: the rotating shaft (1.13) rotates by 90 degrees, so that the third liquid transferring gun (1.3) is transferred to the position below the gun head withdrawing module (5), and the second liquid transferring gun (1.2) is transferred to the position above the sample feeding module (4); the fourth liquid transferring gun (1.4) is transferred to the position above the gun head entering module (2), and the first liquid transferring gun (1.1) is transferred to the position above the upper liquid module (3); the first pipetting gun (1.1) does not absorb any liquid at this time; the sample introduction module (4) rises to enable a gun tube (2.8) of the second pipette (1.2) to contact the bottom of the open test tube (4.12), and liquid in the open test tube (4.12) is continuously sucked and discharged by controlling the continuous rising and falling of a mandril (6.4) of the second liquid suction and discharge module (7), so that the PBS-T solution and the magnetic beads after magnetic separation are uniformly mixed; an execution plate (5.3) of the gun head withdrawing module (5) moves downwards to push a push plate of a third liquid transferring gun (1.3), so that a gun barrel (2.8) falls into a collecting tank (8) below; the gun head feeding module (2) rotates and lifts the gun head disc (2.11) to enable the fourth liquid transferring gun (1.4) to complete gun tube loading operation in the step S2;
s12: after the liquid in the open test tube (4.12) is uniformly mixed, performing the magnetic separation operation of step S8;
s13: the rotating shaft (1.13) rotates by 90 degrees, so that the first liquid transferring gun (1.1) is transferred to the upper part of the sample introduction module (4), the fourth liquid transferring gun (1.4) is transferred to the upper part of the upper liquid module (3), the third liquid transferring gun (1.3) is transferred to the upper part of the gun head entering module (2), and the second liquid transferring gun (1.2) is transferred to the lower part of the gun head withdrawing module (5);
controlling the sample introduction module (4) to ascend, enabling a barrel (2.8) of the first liquid transfer gun (1.1) to be in contact with the bottom of the open test tube (4.12), compressing the first liquid transfer gun (1.1) by controlling a mandril (6.4) of the second liquid suction and discharge module (7), and then ascending, and enabling separated clear liquid to be sucked into the barrel (2.8) of the first liquid transfer gun (1.1); an execution plate (5.3) of the gun head withdrawing module (5) moves downwards to push a push plate of a second liquid transferring gun (1.2) to move, so that a gun barrel (2.8) falls into a collecting tank (8) below; the gun head feeding module (2) rotates the gun head disc (2.11) to enable the third liquid transferring gun (1.3) to face an unused gun barrel (2.8), and the gun head disc (2.11) is lifted to enable the third liquid transferring gun (1.3) to complete gun tube loading operation in the step S2;
the first rotating disc (3.14) of the upper liquid module (3) rotates by 90 degrees, so that the gun tube (2.8) of the fourth liquid transferring gun (1.4) is aligned to the third reagent bottle (3.3), after the piston of the fourth liquid transferring gun (1.4) is downwards compressed by the first liquid absorbing and discharging module (6) through the ejector rod (6.4), the ejector rod (6.4) rises, and at the moment, the fourth liquid transferring gun (1.4) absorbs PBS-BSA liquid with the required measuring range;
s14: the rotating shaft (1.13) rotates by 90 degrees, so that the first liquid transferring gun (1.1) is transferred to the position below the gun head withdrawing module (5), the second liquid transferring gun (1.2) is transferred to the position above the gun head feeding module (2), the third liquid transferring gun (1.3) is transferred to the position above the upper liquid module (3), and the fourth liquid transferring gun (1.4) is transferred to the position above the sample feeding module (4);
an execution plate (5.3) of the gun head withdrawing module (5) moves downwards to push a push plate of the first liquid transferring gun (1.1) to move downwards, so that a gun barrel (2.8) is pushed to fall into a collecting tank (8) below; the gun head feeding module (2) rotates the gun head disc (2.11) to enable the second liquid-transferring gun (1.2) to face an unused gun barrel (2.8), and the gun head disc (2.11) is lifted to enable the second liquid-transferring gun (1.2) to complete gun tube loading operation in the step S2; the sample injection module (4) ascends to enable a gun barrel (2.8) of the fourth liquid transfer gun (1.4) to contact the bottom of the open test tube (4.12), a mandril (6.4) of the second liquid suction and discharge module (7) is controlled to ascend and descend continuously, liquid in the open test tube (4.12) is sucked and discharged continuously, the PBS-BSA solution in the open test tube (4.12) and the solution after magnetic separation in the step S13 are mixed uniformly, and the sample injection module (4) descends to an initial position;
the first rotating disc (3.14) of the upper liquid module (3) rotates by 90 degrees, so that a reagent bottle (3.4) of the fourth number is aligned to the third liquid transferring gun (1.3), after the first liquid absorbing and discharging module (6) downwards compresses the piston of the third liquid transferring gun (1.3) through the ejector rod (6.4), the ejector rod (6.4) ascends, and at the moment, the third liquid transferring gun (1.3) absorbs the quantum dot solution with the required measuring range;
s15: the rotating shaft (1.13) rotates by 90 degrees, the first liquid transferring gun (1.1) is transferred to the position above the gun head entering module (2), the second liquid transferring gun (1.2) is transferred to the position above the upper liquid module (3), the third liquid transferring gun (1.3) is transferred to the position above the sample introduction module (4), and the fourth liquid transferring gun (1.4) is transferred to the position below the gun head withdrawing module (5); the gun head feeding module (2) rotates the gun head disc (2.11) to enable the first liquid-transferring gun (1.1) to face an unused gun barrel (2.8), and the gun head disc (2.11) is lifted to enable the first liquid-transferring gun (1.1) to complete gun tube loading operation of the step S2; an execution plate (5.3) of the gun head withdrawing module (5) moves downwards to push a push plate of a fourth liquid transferring gun (1.4) to move, so that a gun barrel (2.8) below the push plate is pushed to fall into a collecting tank (8);
controlling the sample injection module (4) to ascend to enable a gun barrel (2.8) of the third liquid transfer gun (1.3) to be in contact with the bottom of the open test tube (4.12), controlling a mandril (6.4) of the second liquid suction and discharge module (7) to ascend and descend continuously to enable liquid in the open test tube (4.12) to be sucked and discharged continuously, mixing the quantum dot solution, the PBS-BSA solution and the solution subjected to the magnetic separation in the step S13 uniformly in the open test tube (4.12), and descending the sample injection module (4) to an initial position;
s16: after the liquid in the open test tube (4.12) is uniformly mixed, carrying out step S8 magnetic separation operation;
s17: the rotating shaft (1.13) rotates by 90 degrees, so that the first liquid transferring gun (1.1) is transferred to the upper part of the upper liquid module (3), the second liquid transferring gun (1.2) is transferred to the sample introduction module (4), the third liquid transferring gun (1.3) is transferred to the lower part of the gun head withdrawing module (5), and the fourth liquid transferring gun (1.4) is transferred to the upper part of the gun head entering module (2);
controlling the sample introduction module (4) to ascend to enable a gun tube (2.8) of the second liquid transferring gun (1.2) to contact the bottom of the open test tube (4.12), and controlling a mandril (6.4) of the second liquid sucking and discharging module (7) to compress the second liquid transferring gun (1.2) downwards and descend, and then enabling the mandril (6.4) to ascend to enable separated clear liquid to be sucked into the second liquid transferring gun (1.2);
an execution plate (5.3) of the gun head withdrawing module (5) moves downwards to push a push plate of a third liquid transferring gun (1.3), so that a gun barrel (2.8) below the push plate is pushed to drop into a collecting tank (8); the gun head feeding module (2) rotates the gun head disc (2.11) to enable the fourth liquid-transferring gun (1.4) to face the unused gun barrel (2.8), and the gun head disc (2.11) is lifted to enable the fourth liquid-transferring gun (1.4) to complete the gun tube loading operation of the step S2;
the third reagent bottle (3.3) is aligned to the first liquid transferring gun (1.1) by rotating the first rotary disc (3.14) of the upper liquid module (3), the first liquid absorbing and discharging module (6) downwards compresses a piston of the first liquid transferring gun (1.1) through a push rod (6.4), the push rod (6.4) rises, and at the moment, the first liquid transferring gun (1.1) absorbs PBS-BSA liquid with a required measuring range;
s18: the rotating shaft (1.13) rotates by 90 degrees, so that the second liquid transferring gun (1.2) is transferred to the position below the gun head withdrawing module (5), the first liquid transferring gun (1.1) is transferred to the position above the sample feeding module (4), the third liquid transferring gun (1.3) is transferred to the position above the gun head feeding module (2), and the fourth liquid transferring gun (1.4) is transferred to the position above the upper liquid module (3);
the sample injection module (4) ascends to enable a gun tube (2.8) of the first liquid transfer gun (1.1) to contact the bottom of the open test tube (4.12), a mandril (6.4) of the second liquid suction and discharge module (7) is controlled to ascend and descend continuously, liquid in the open test tube (4.12) is sucked and discharged continuously, the PBS-BSA solution and the solution after magnetic separation in the step S18 are mixed uniformly, and the sample injection module (4) descends to an initial position;
an execution plate (5.3) of the gun head withdrawing module (5) moves downwards, and a gun barrel (2.8) connected with a push plate is dropped into a collection tank (8) by pushing the push plate of the second liquid transferring gun (1.2);
s19: the rotating shaft (1.13) rotates by 90 degrees, so that the first liquid transferring gun (1.1) is transferred to the position below the gun head withdrawing module, the second liquid transferring gun (1.2) is transferred to the position above the gun head feeding module (2), the third liquid transferring gun (1.3) is transferred to the position above the upper liquid module (3), and the fourth liquid transferring gun (1.4) is transferred to the position above the sample feeding module; the sample introduction module (4) ascends to enable a gun tube (2.8) of the fourth pipette (1.4) to contact the bottom of the open test tube (4.12), the ejector rod (6.4) of the second liquid suction and discharge module (7) is controlled to compress the fourth pipette (1.4) downwards, then the ejector rod (6.4) ascends, and at the moment, the fourth pipette (1.4) absorbs liquid in the open test tube (4.12) with the required measuring range;
a second rotating disc (4.4) of the sample introduction module (4) rotates 180 degrees, so that a second test tube rack (4.8) is aligned to a fourth liquid transfer gun (1.4), and then a second liquid suction and discharge module (7) is compressed to the fourth liquid transfer gun (1.4) through a mandril (6.4) to discharge liquid in the liquid transfer gun;
a second rotating disc (4.4) of the sample introduction module (4) rotates for 180 degrees, and a second test tube (4.6) is taken out for subsequent detection of the microplate reader/spectrometer;
s20: the rotating shaft (1.13) rotates by 90 degrees, so that the fourth liquid gun (1.4) is rotated to the position below the gun head withdrawing module (5), the third liquid transferring gun (1.3) is rotated to the position above the sample feeding module (4), the second liquid transferring gun (1.2) is rotated to the position above the upper liquid module (3), and the first liquid transferring gun (1.1) is rotated to the position above the gun head feeding module (2);
s21: and resetting each module to the initial position, and ending.
6. The pretreatment method for chicken extract as claimed in claim 5, wherein the initial positions of the modules are: the initial position of the pipette head module (2) is that the upper end of a first baffle (2.15) and the upper end of a first initial positioning block (2.6) are contacted and superposed, the initial position of the upper liquid module (3) is that a stop block arranged on the upper part of a bottle seat (3.17) is contacted with the upper end of a second initial positioning block (3.8), the initial position of the sample feeding module (4) is that the stop block on the upper part of a second test tube rack (4.8) is contacted with the upper end of a third initial positioning block (4.7), the initial position of the pipette gun module (1) is that a photosensitive center of a photoelectric sensor (1.5) is aligned with one photoelectric sensor detection plate (1.6), the initial position of the pipette head module (5) is that a seventh slide block (5.8) moves to the upper end face of a sixth top plate (5.9), and the initial position of the pipette module is that a fifth slide block (6.9) moves to the upper end face.
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