CN221166520U - Nucleic acid extraction appearance and nucleic acid detection all-in-one - Google Patents

Abstract

The application belongs to the field of molecular detection, and particularly relates to a nucleic acid extraction instrument and a nucleic acid detection integrated machine. The consumable carrier is used for placing prepackaged consumables; the magnetic suction module is positioned below the consumable carrier and is provided with a magnetic suction piece which can be close to or far away from the reaction cavity; the pipetting module is used for transferring liquid between the sample carrier and the consumable carrier; the waste liquid removing module comprises a liquid suction pipeline and a liquid suction head arranged on the liquid suction pipeline, the liquid suction pipeline is used for communicating a waste liquid container, the liquid suction head can move relative to the consumable carrier, and waste liquid in the reaction cavity can be sucked to the waste liquid container through negative pressure. The waste liquid removing module is matched with the prepackaged consumable, so that the time for removing waste liquid, adding reagents and replacing consumable can be greatly shortened, the extraction time is shortened, and the nucleic acid extraction efficiency is improved.

Description

Nucleic acid extraction appearance and nucleic acid detection all-in-one

Technical Field

The application relates to the technical field of molecular detection, in particular to a nucleic acid extractor and a nucleic acid detection integrated machine.

Background

The substance of the nucleic acid detection is a viral nucleic acid, and the nucleic acid detection is to find whether a foreign invading viral nucleic acid exists in a respiratory tract sample, blood or feces of a patient to determine whether the patient is infected with the virus.

Nucleic acid detection requires first extracting nucleic acid and then detecting the extracted nucleic acid. At present, magnetic bead method nucleic acid extraction instruments in the market divide a magnetic bead method into an upper magnetic attraction method and a lower magnetic attraction method according to the positions of sucking magnetic beads, wherein the upper magnetic attraction method is to arrange a magnetic attraction component above a reagent strip component, the magnetic attraction component adsorbs the magnetic beads on the magnetic attraction component from the upper part of a reagent bin and transfers the magnetic beads, the upper magnetic attraction method is adopted to extract the magnetic beads for relatively short time, but the magnetic beads have pollution risks in the adsorption and transfer processes; the lower magnetic attraction method is to set a magnetic attraction component at the bottom of the reagent strip component, adsorb magnetic beads at the bottom of the reagent bin by the magnetic attraction component, and remove the washing liquid in the reagent bin from the upper part of the reagent bin, specifically, in the related art, the washing liquid removal flow is generally as follows: the liquid in the reagent bin is sucked through the liquid-transferring gun head, after the liquid is sucked by the liquid-transferring gun head, the liquid-transferring gun head and the liquid adsorbed in the liquid-transferring gun head are driven by the moving mechanism to move to the upper part of the waste liquid tank for collecting the waste liquid, and finally the liquid in the liquid-transferring gun head is poured into the waste liquid tank. Although the down magnetic adsorption method has small pollution possibility, the time for removing waste liquid and adding the reagent is longer in the extraction process, so that the nucleic acid extraction time is relatively longer.

Disclosure of utility model

The embodiment of the application provides a nucleic acid extraction instrument and a nucleic acid detection integrated machine, which aim to ensure anti-pollution capability and simultaneously consider extraction efficiency.

To this end, according to one aspect of the present application, there is provided a nucleic acid extraction instrument including a base plate, and a sample carrier, a consumable carrier, a magnetic attraction module, a pipetting module, and a waste liquid removal module provided on the base plate;

the sample carrier is used for placing a sample tube;

The consumable carrier is used for placing pre-packaging consumable, the pre-packaging consumable comprises a consumable body, a reaction cavity and a reagent cavity, the reaction cavity and the reagent cavity are arranged on the consumable body, and the reagent cavity is filled with sample processing reagent;

The magnetic suction module is positioned below the consumable carrier and is provided with a magnetic suction piece which can be close to or far away from the reaction cavity;

The pipetting module is used for transferring liquid between the sample carrier and the consumable carrier;

The waste liquid removing module comprises a liquid suction pipeline and a liquid suction head arranged on the liquid suction pipeline, the liquid suction pipeline is used for communicating a waste liquid container, the liquid suction head can move relative to the consumable carrier, and waste liquid in the reaction cavity can be sucked to the waste liquid container through negative pressure.

Optionally, the nucleic acid extraction instrument further comprises a carrier base, the carrier base is slidably arranged on the bottom plate, and the sample carrier, the consumable carrier and the magnetic attraction module are arranged on the carrier base.

Optionally, the magnetic attraction module further comprises a driving mechanism and a heating body, the magnetic attraction piece and the heating body are both arranged on the driving mechanism, and the magnetic attraction piece and the heating body can be close to or far away from the reaction cavity under the action of the driving mechanism.

Optionally, the magnetic attraction piece and the heating body are arranged side by side, a plurality of prepackaged consumable materials can be placed side by side on the consumable material carrier, a plurality of magnetic attraction grooves are formed in the magnetic attraction piece at intervals, a plurality of heating grooves are formed in the heating body at intervals, and the prepackaged consumable materials, the magnetic attraction grooves and the heating grooves are respectively in one-to-one correspondence in the arrangement direction of the magnetic attraction piece and the heating body;

The magnetic attraction piece and the heating body can move in the arrangement direction of the magnetic attraction piece and the heating body and in the height direction of the reaction cavity under the action of the driving mechanism.

Optionally, be provided with support frame and drive assembly on the bottom plate, the support frame slides along first direction and sets up on the bottom plate, drive assembly connects in the support frame for drive support frame removes in first direction, and pipetting module and waste liquid remove the module and all set up on the support frame, and pipetting module and waste liquid remove the module along the in-process of first direction removal along with the support frame, can be respectively with sample carrier and consumptive material carrier relative in the direction of height of support frame.

Optionally, the drive assembly sets up in the one side that the bottom plate deviates from the support frame, and drive end of drive assembly is connected with the adaptor, and the adaptor is connected in the one end that the support frame is close to the bottom plate.

Optionally, the pipetting module includes first straight line module and multichannel pipetting module, and first straight line module sets up on the support frame, and multichannel pipetting module sets up on first straight line module and can move in the direction of height of support frame under the effect of first straight line module, and multichannel pipetting module has a plurality of installation heads that are used for detachably to install the rifle head, and a plurality of installation heads set gradually in the second direction, and multichannel pipetting module can provide each rifle head and absorb or spit the effort of solution, and the second direction is all crisscross with the direction of height of first direction and support frame.

Optionally, the waste liquid removing module further comprises a second linear module, the second linear module is arranged on the support frame, the liquid suction pipeline is arranged on the second linear module and can move in the height direction of the support frame under the action of the second linear module, the liquid suction pipeline is provided with a plurality of connectors which are sequentially arranged in the second direction, each connector is detachably connected with one liquid suction head, the liquid suction heads comprise gun heads, and the second direction is intersected with the first direction and the height direction of the support frame.

Optionally, the consumable carrier further has a gun head placement position for placing a gun head.

According to another aspect of the present application, there is provided a nucleic acid detecting integrated machine comprising a PCR detector and the nucleic acid extractor as above, the PCR detector being disposed on a base plate.

The nucleic acid extractor and the nucleic acid detection all-in-one machine provided by the application have the beneficial effects that: compared with the prior art, the nucleic acid extractor has the advantages that the magnetic attraction module is arranged below the consumable carrier, the magnetic attraction piece is utilized to adsorb the magnetic beads in the reaction cavity on the inner wall of the reaction cavity in the process of extracting nucleic acid, then the liquid in the reaction cavity is removed from the upper part of the reaction cavity through the waste liquid removing module, and the magnetic beads are adsorbed on the bottom of the reaction cavity by adopting the magnetic attraction module positioned below the consumable carrier, so that the transfer of the magnetic beads outside the reaction cavity does not exist compared with an upper adsorption extraction method, and the nucleic acid extractor has the advantage of small pollution possibility in the process of extracting nucleic acid; simultaneously, the consumable carrier is used for placing the consumptive material that has packaged in advance sample processing reagent, and the waste liquid removes the module and can directly be with the waste liquid suction to the waste liquid container in the extraction process reaction chamber through the mode of negative pressure suction, has saved steps such as the absorption of waste liquid, transfer and spitting out, and the waste liquid removes the module cooperation and packages the consumptive material in advance, can reduce by a wide margin and remove the waste liquid, add the time of reagent and change the consumptive material, and then shortens extraction time, improves nucleic acid extraction efficiency, guarantees nucleic acid detection all-in-one's detection precision and efficiency.

Drawings

In order to more clearly illustrate the embodiments of the application or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the application, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

Wherein:

FIG. 1 is a schematic diagram showing the overall structure of a nucleic acid extractor according to an embodiment of the present application;

FIG. 2 is a schematic diagram showing the connection among a bottom plate, a sample carrier, a consumable carrier, a magnetic attraction module and a carrier base of a nucleic acid extraction instrument according to an embodiment of the present application;

FIG. 3 is a schematic diagram showing the structure of a magnetic attraction module in a nucleic acid extractor according to an embodiment of the present application;

FIG. 4 is a schematic diagram showing the overall structure of a nucleic acid isolation apparatus according to an embodiment of the present application;

FIG. 5 is a schematic diagram showing the structure of a pipetting module in a nucleic acid extractor according to an embodiment of the application;

FIG. 6 is a schematic view of a pipetting module shown in FIG. 5 from another perspective;

FIG. 7 is a schematic diagram showing a structure of a waste liquid removing module in a nucleic acid extracting apparatus according to an embodiment of the present application;

FIG. 8 is a schematic diagram of another view of an embodiment of the present application;

FIG. 9 is a schematic diagram showing the overall structure of a nucleic acid detecting integrated machine according to an embodiment of the present application.

Description of main reference numerals:

10. A nucleic acid extraction instrument;

20. A PCR detector;

30. A sample tube;

40. Pre-packaging consumable materials; 41. a reaction chamber;

50. a gun head;

100. a bottom plate; 101. a bar-shaped through hole;

200. A sample carrier;

300. A consumable carrier;

400. a carrier base;

500. A magnetic attraction module; 510. a driving mechanism; 511. a first linear module; 512. a second linear module; 520. a magnetic attraction piece; 521. a magnetic attraction groove; 530. a heating body; 531. a heating tank;

600. a support frame; 610. a support plate; 620. a mounting plate;

700. A drive assembly; 710. a motor; 720. a screw rod; 730. a drive nut; 740. a connecting rod; 750. an adapter;

800. A pipetting module; 810. a first linear module; 820. a multichannel pipetting assembly; 821. a mounting head;

900. A waste liquid removal module; 910. a second linear module; 920. a liquid suction pipeline; 921. and (3) a joint.

Detailed Description

In order that the application may be readily understood, a more complete description of the application will be rendered by reference to the appended drawings. Preferred embodiments of the present application are shown in the drawings. This application may, however, be embodied in many other different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

It will be understood that when an element is referred to as being "mounted" or "disposed" on another element, it can be directly on the other element or be indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or be indirectly connected to the other element.

It is to be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are merely for convenience in describing and simplifying the description based on the orientation or positional relationship shown in the drawings, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus are not to be construed as limiting the application.

Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present application, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used herein in the description of the application is for the purpose of describing particular embodiments only and is not intended to be limiting of the application.

It should be further noted that, in the embodiments of the present application, the same reference numerals denote the same components or the same parts, and for the same parts in the embodiments of the present application, reference numerals may be given to only one of the parts or the parts in the drawings, and it should be understood that, for other same parts or parts, the reference numerals are equally applicable.

According to one aspect of the present application, an embodiment of the present application provides a nucleic acid extraction apparatus, as shown in fig. 1, the nucleic acid extraction apparatus 10 includes a base plate 100, and a sample carrier 200, a consumable carrier 300, a magnetic attraction module 500, a pipetting module 800, and a waste liquid removal module 900 provided on the base plate 100.

The sample carrier 200 is used for placing the sample tube 30; the consumable carrier 300 is used for placing a prepackaged consumable 40, the prepackaged consumable 40 comprises a consumable body, a reaction cavity 41 and a reagent cavity, wherein the reaction cavity 41 and the reagent cavity are arranged on the consumable body, and the reagent cavity is filled with a sample processing reagent; the magnetic attraction module 500 is located below the consumable carrier 300, the magnetic attraction module 500 is provided with a magnetic attraction piece 520 which can be close to or far away from the reaction cavity 41, and the magnetic attraction piece 520 is provided with an adsorption state for adsorbing the magnetic beads in the reaction cavity 41 to the inner wall of the reaction cavity and a release state for releasing the adsorption of the magnetic beads in the reaction cavity 41; pipetting module 800 is used to transfer liquid between sample carrier 200 and consumable carrier 300; the waste liquid removing module 900 includes a liquid suction line 920 and a liquid suction head (not shown in the drawing) disposed on the liquid suction line 920, the liquid suction line 920 being for communicating with a waste liquid container (not shown in the drawing), the liquid suction head being movable relative to the consumable carrier 300 and being capable of sucking the waste liquid in the reaction chamber 41 to the waste liquid container by negative pressure.

In the embodiment of the application, the magnetic attraction module 500 is arranged below the consumable carrier 300, the magnetic beads in the reaction cavity 41 can be adsorbed on the inner wall of the reaction cavity 41 in the process of extracting nucleic acid by using the magnetic attraction piece 520, then the liquid in the reaction cavity 41 is removed from the upper side of the reaction cavity 41 by the waste liquid removing module 900, and the magnetic beads are adsorbed on the bottom of the reaction cavity 41 by using the magnetic attraction module 500 positioned below the consumable carrier 300, so that compared with an upper adsorption extraction method, the nucleic acid extraction device 10 has the advantage that the possibility of pollution in the process of extracting nucleic acid is small because the magnetic beads are not transferred outside the reaction cavity 41; meanwhile, the consumable carrier 300 is used for placing the pre-packaged consumable 40, the waste liquid in the reaction cavity 41 can be directly sucked to the waste liquid container by the waste liquid removing module 900 in the extraction process through the negative pressure suction mode, the steps of sucking, transferring, spitting out and the like of the waste liquid are omitted, the waste liquid removing module 900 is matched with the pre-packaged consumable 40 on the consumable carrier 300, the time for adding reagents, replacing consumables and removing the waste liquid can be greatly shortened, the extraction time is further shortened, and the nucleic acid extraction efficiency is improved.

In actual use, the sample tube 30 containing the sample solution is placed on the sample carrier 200, the prepackaged consumable 40 is placed on the consumable carrier 300, and the pipetting module 800 transfers the sample solution in the sample tube 30 into the reaction chamber 41 of the prepackaged consumable 40. The pipetting module 800 transfers the lysate in the corresponding reagent chamber on the pre-packaged consumable 40 into the reaction chamber 41, lysing the cells and releasing the nucleic acids. Then, the pipetting module 800 transfers the magnetic bead suspension in the corresponding reagent chamber on the pre-packaged consumable 40 into the reaction chamber 41, and adsorbs nucleic acid by using the magnetic beads. The magnetic attraction piece 520 of the magnetic attraction module 500 is close to the reaction cavity 41, provides an external magnetic field, adsorbs the magnetic beads adsorbed with the nucleic acid on the inner wall of the reaction cavity 41, and the waste liquid removing module 900 removes the lysate in the reaction cavity 41 through the liquid suction head. Then, the pipetting module 800 transfers the washing liquid in the corresponding reagent cavity on the prepackaged consumable 40 into the reaction cavity 41, firstly removes the external magnetic field to mix the magnetic beads with the washing liquid, washes out impurities, then provides the external magnetic field to adsorb the magnetic beads on the inner wall of the reaction cavity 41, and the waste liquid removing module 900 removes the washing liquid in the reaction cavity 41 through the liquid suction head. Finally, the pipetting module 800 transfers the eluent in the corresponding reagent chamber on the prepackaged consumable 40 into the reaction chamber 41, removes the external magnetic field to mix the magnetic beads and the eluent, separates the combined nucleic acid from the magnetic beads, mixes the combined nucleic acid into the eluent, and obtains the purified nucleic acid by taking the supernatant.

From the above, the pre-packaging consumable 40 is adopted, so that the nucleic acid extractor 10 does not need to replace consumable frequently in the process of extracting nucleic acid, the reagent adding time is shortened, the pre-packaging consumable 40 can complete steps of cracking, washing, eluting and the like, the use is convenient, the waste liquid removing module 900 directly pumps waste liquid (namely cracking liquid and washing liquid after use) in the reaction cavity 41 to the waste liquid container in a negative pressure pumping mode, the steps of sucking, spitting, transferring and the like are omitted, the waste liquid is removed through consumable reagent pre-packaging and direct suction, the reagent adding time, consumable changing time and waste liquid removing time are greatly shortened, the pollution prevention capability is ensured, and meanwhile the extraction efficiency is also ensured.

It should be noted that, the prepackaging consumable 40 is provided with a plurality of reagent cavities, the plurality of reagent cavities are respectively filled with a lysis solution, a magnetic bead suspension, a washing solution and an eluent, the reaction cavity 41 and the plurality of reagent cavities are arranged in a straight line on the consumable body, and the prepackaging consumable 40 can adopt the prior art and is not described herein again.

It is to be understood that, the way of implementing the negative pressure for the liquid suction head on the liquid suction removal module 900 may be implemented by connecting the liquid suction pipe 920 to the liquid suction container having the negative pressure inside, or by providing a vacuum pump between the liquid suction pipe 920 and the liquid suction container, which is not limited herein.

In one embodiment, as shown in fig. 1-2, the nucleic acid extraction apparatus 10 further comprises a carrier base 400, the carrier base 400 is slidably disposed on the bottom plate 100, and the sample carrier 200, the consumable carrier 300, and the magnetic attraction module 500 are disposed on the carrier base 400.

Through the arrangement, the sample carrier 200, the consumable carrier 300 and the magnetic attraction module 500 which are positioned on the carrier base 400 can be driven to move together by pushing and pulling the carrier base 400, so that the sample carrier 200 and the consumable carrier 300 are staggered with the pipetting module 800 and the waste liquid removing module 900 in the vertical direction, and the sample tube 30 and the prepackaged consumable 40 can be placed on the sample carrier 200 and the consumable carrier 300 respectively.

Further, a limiting piece for limiting the sliding limit position of the carrier base 400 is further arranged on the base, a handle is further arranged on the carrier base 400, and the handle is convenient for an operator to apply force when the carrier base 400 is pushed and pulled.

In one embodiment, as shown in fig. 2-3, the magnetic attraction module 500 further includes a driving mechanism 510 and a heating body 530, where the magnetic attraction member 520 and the heating body 530 are disposed on the driving mechanism 510, and the magnetic attraction member 520 and the heating body 530 can approach or depart from the reaction cavity 41 under the action of the driving mechanism 510.

Through setting up like this for this magnetism is inhaled the subassembly and is still possessed the heating function, can be applicable to the condition that needs to heat reactant.

In addition, the magnetic attraction piece 520 and the heating module are arranged on the same driving mechanism 510, and the magnetic attraction piece and the heating body 530 are controlled to move in a three-dimensional space by using one driving mechanism 510 to carry out magnetic attraction operation and heating operation on the reaction cavity 41, so that the number of the driving mechanisms 510 is reduced, the space is reasonably utilized, the magnetic attraction piece 520 and the heating module synchronously move, the synergy of heating and magnetic attraction functions in the magnetic attraction extraction process is ensured, the detection sensitivity is improved, the integral structure of the equipment is optimized, the space occupation rate of the equipment is reduced, and the miniaturization design of the whole nucleic acid extractor 10 is facilitated.

In a specific embodiment, as shown in fig. 2 and 3, the magnetic attraction pieces 520 and the heating body 530 are arranged side by side, a plurality of pre-packaged consumable pieces 40 can be placed side by side on the consumable carrier 300, a plurality of magnetic attraction grooves 521 are arranged on the magnetic attraction pieces 520 at intervals, a plurality of heating grooves 531 are arranged on the heating body 530 at intervals, and the plurality of pre-packaged consumable pieces 40, the plurality of magnetic attraction grooves 521 and the plurality of heating grooves 531 are respectively in one-to-one correspondence with the arrangement direction of the magnetic attraction pieces 520 and the heating body 530; the magnetic attraction member 520 and the heating body 530 are capable of moving in the arrangement direction of the magnetic attraction member 520 and the heating body 530 and in the height direction of the reaction chamber 41 by the driving mechanism 510.

Through the arrangement, the magnetic attraction piece 520 and the heating body 530 in the magnetic attraction module 500 can respectively and simultaneously carry out magnetic attraction and heating operation on a plurality of reaction cavities 41 under the action of the driving mechanism 510, and the multi-human nucleic acid extraction operation can be simultaneously realized by matching the pipetting module 800 with the multi-liquid-suction head waste liquid removal module 900 with the multi-channel pipetting module 820, so that the high-integration design is realized, the size of the whole machine is greatly reduced, and by way of example, 8 sample tubes 30 are placed side by side on the sample carrier 200 in the figure, 8 pre-packaging consumable 40 are placed side by side on the consumable carrier 300, 8 magnetic attraction grooves 521 are shown on the magnetic attraction piece 520, and 8 heating grooves 531 are shown on the heating body 530.

For convenience of description, an X-Y-Z three-dimensional coordinate system is established in the figure, wherein the arrangement direction of the magnetic attraction member 520 and the heating body 530 is the direction in which the X axis is located. The plurality of magnetic attraction grooves 521 are arranged on the magnetic attraction piece 520 at intervals in the direction of the Y axis, the plurality of heating grooves 531 are arranged on the heating body 530 at intervals in the direction of the Y axis, the plurality of pre-packaged consumable materials 40 are arranged on the consumable material carrier 300 side by side in the direction of the Y axis, and the plurality of sample tubes 30 are arranged on the sample carrier 200 side by side in the direction of the Y axis. The height direction of the sample tube 30 and the reaction chamber 41 is the direction in which the Z axis is located.

Specifically, the magnetic attraction member 520 may employ a permanent magnet or an electromagnet, and the heating body 530 may include a heating portion (e.g., an electrothermal film or an electrothermal wire) and a heat conducting portion (e.g., a metal block) connected to each other, and the heating groove 531 is provided on the heat conducting portion. The magnetic attraction groove 521 and the heating groove 531 are both V-shaped grooves, so as to better attach to the reaction cavity 41 with the bottom being V-shaped, and ensure the magnetic attraction and heating effects.

In a more specific embodiment, as shown in fig. 2-3, the driving mechanism 510 includes a first linear module 511 and a second linear module 512, the second linear module 512 is disposed on the first linear module 511, the magnetic attraction member 520 and the heating body 530 are disposed on the second linear module 512, and the magnetic attraction member 520 and the heating body 530 can move in the arrangement direction of the magnetic attraction member 520 and the heating body 530 under the action of the first linear module 511, and move in the height direction of the reaction chamber 41 under the action of the second linear module 910.

It will be appreciated that the mutual switching of the magnetic attraction operation and the heating operation can be achieved by moving in the arrangement direction of the magnetic attraction member 520 and the heating body 530; in the height direction of the reaction chamber 41, up-and-down movement may be achieved to fit or be away from the bottom of the reaction chamber 41. By the arrangement, the driving mechanism 510 only needs to perform driving in two directions, and the structure is simple and the control is convenient.

The first linear module 511 and the second linear module 512 may be synchronous belt linear modules, ball screw linear modules, rack and pinion sliding table modules, or even air cylinders or electric cylinders.

In one embodiment, as shown in fig. 1 and 4, a support frame 600 and a driving assembly 700 are disposed on the bottom plate 100, the support frame 600 is slidably disposed on the bottom plate 100 along a first direction, the driving assembly 700 is connected to the support frame 600 and is used for driving the support frame 600 to move in the first direction, the pipetting module 800 and the waste liquid removing module 900 are both disposed on the support frame 600, and the pipetting module 800 and the waste liquid removing module 900 can be respectively opposite to the sample carrier 200 and the consumable carrier 300 in the height direction of the support frame 600 during the process of moving along the first direction along with the support frame 600.

By disposing the pipetting module 800 and the waste liquid removal module 900 on the same support frame 600, the support frame 600 is driven to move in the first direction by the driving assembly 700. On the one hand, the pipetting module 800 and the waste liquid removing module 900 are simultaneously arranged on the bottom plate 100 by taking the supporting frame 600 as a carrier, and the structure is simple and compact; on the other hand, the driving assembly 700 drives the support frame 600 to move, so that the pipetting module 800 and the waste liquid removing module 900 are driven to be respectively opposite to the sample carrier 200 and the consumable carrier 300 in the height direction of the support frame 600, and the pipetting module 800 and the waste liquid removing module 900 only need to perform corresponding lifting operation, which is beneficial to simplifying the driving parts of the pipetting module 800 and the waste liquid removing module 900, and meanwhile, the situation that the pipetting module 800 and the waste liquid removing module 900 are easy to generate motion interference in the process of independent motion in space can be avoided.

Specifically, the support frame 600 includes two support plates 610 and two mounting plates 620, the two support plates 610 are respectively disposed on opposite sides of the bottom plate 100 in the first direction, the two mounting plates 620 are disposed between the two support plates 610 at intervals in the first direction, the two mounting plates 620 are respectively used as mounting carriers of the pipetting module 800 and the waste liquid removing module 900, and one ends of the two support plates 610, which are close to the bottom plate 100, can be respectively connected with the bottom plate 100 through linear guide rails extending along the first direction, so as to realize that the support frame 600 is slidably disposed on the bottom plate 100 along the first direction.

In the X-Y-Z three-dimensional coordinate system in the figure, the first direction is the direction in which the X axis is located, the second direction in the embodiment described below is the direction in which the Y axis is located, and the plurality of pre-packaged consumables 40 and the plurality of sample tubes 30 are sequentially disposed in the direction in which the Y axis is located, respectively, and the height direction of the support frame 600 is the direction in which the Z axis is located.

In a specific embodiment, as shown in fig. 4, the driving assembly 700 is disposed on a side of the base plate 100 away from the supporting frame 600, the driving end of the driving assembly 700 is connected with the adapter 750, and the adapter 750 is connected to an end of the supporting frame 600 near the base plate 100.

Because sample carrier 200, consumptive material carrier 300, magnetism inhale module 500, pipetting module 800 and waste liquid remove module 900 all set up the top surface at bottom plate 100, set up drive assembly 700 in the bottom plate 100 one side that deviates from support frame 600 (i.e. the bottom surface of bottom plate 100), can avoid drive assembly 700 to cause the interference to the structure that sets up at bottom plate 100 top surface, plan the space simultaneously rationally, optimize the overall structure of equipment, reduce the space occupancy of equipment.

In one implementation, the drive assembly 700 includes a motor 710, a lead screw 720, a drive nut 730, and a connecting rod 740, the lead screw 720 being rotatably disposed about its own axis to the base plate 100, and the axial direction of the lead screw 720 being parallel to the first direction. The motor 710 is fixed to the base plate 100 and is in driving connection with the screw 720 for driving the screw 720 to rotate about its own axis. The transmission nut 730 is in threaded connection with the lead screw 720, so that when the lead screw 720 rotates around the axis of the lead screw 720, the transmission nut 730 can be driven to move along the first direction relative to the bottom plate 100, the connecting rod 740 is fixedly connected with the transmission nut 730, the bottom plate 100 is provided with a strip-shaped through hole 101 penetrating the bottom plate 100 up and down, the length direction of the strip-shaped through hole 101 is parallel to the first direction, the adapter 750 is arranged in the strip-shaped through hole 101, one end of the adapter 750 is connected with the support frame 600, and the other end is connected with the connecting rod 740.

The driving assembly 700 adopts a screw pair formed by the screw 720 and the transmission nut 730 to drive the support frame 600 to move relative to the base, and has simple structure, higher movement precision and convenient control. Of course, in other implementations, other driving structures, such as a belt driving structure, a rack and pinion driving structure, etc., may be used for the driving assembly 700, as long as the driving support 600 can move along the first direction relative to the base, which is not limited herein.

In a specific embodiment, as shown in fig. 1 and 5-6, the pipetting module 800 includes a first linear module 810 and a multi-channel pipetting assembly 820, the first linear module 810 is disposed on the support frame 600, the multi-channel pipetting assembly 820 is disposed on the first linear module 810 and can move in a height direction of the support frame 600 under the action of the first linear module 810, the multi-channel pipetting assembly 820 has a plurality of mounting heads 821 for detachably mounting gun heads (not shown in the drawings), the plurality of mounting heads 821 are sequentially disposed in a second direction, and the multi-channel pipetting assembly 820 can provide a force for each gun head to suck or discharge a solution (that is, generate a negative pressure for sucking a liquid by the gun head or a positive pressure for discharging a liquid by the gun head), and the second direction intersects with the first direction and the height direction of the support frame 600.

When the pipetting module 800 transfers liquid between the sample carrier 200 and the consumable carrier 300, it is necessary to go through the following steps: 1. the driving assembly 700 drives the support 600 to drive the pipetting module 800 to move along the first direction to be opposite to the sample carrier 200 or the consumable carrier 300 in the height direction of the support 600; 2. the first linear module 810 drives the multi-channel pipetting assembly 820 and a gun head (not shown) thereon to descend and insert into the corresponding cavity or sample tube 30 to withdraw fluid; 3. the first linear module 810 drives the multi-channel pipetting assembly 820 and the gun head thereon to be lifted to the corresponding height; 4. the driving assembly 700 drives the support 600 to drive the pipetting module 800 to move along the first direction to be opposite to the sample carrier 200 or the consumable carrier 300 in the height direction of the support 600; 5. the first linear module 810 drives the multi-channel pipetting assembly 820 and the gun head positioned thereon to descend and discharge the liquid in the gun head; 6. the first linear module 810 drives the multi-channel pipetting assembly 820 and the gun head thereon to be lifted to the corresponding height.

Specifically, the first linear module 810 may be a synchronous belt linear module, a ball screw linear module, a rack and pinion sliding module, or even a cylinder or an electric cylinder, which is not limited herein.

Further, pipetting module 800 also includes a gun head removing mechanism coupled to multichannel pipetting assembly 820 and at least partially movable relative to mounting head 821 to disengage the gun head from mounting head 821.

In a specific embodiment, as shown in fig. 1 and fig. 7-8, the waste liquid removing module 900 further includes a second linear module 910, the second linear module 910 is disposed on the support frame 600, the liquid suction pipeline 920 is disposed on the second linear module 910 and can move in the height direction of the support frame 600 under the action of the second linear module 910, the liquid suction pipeline 920 has a plurality of connectors 921 sequentially disposed in the second direction, each connector 921 is detachably connected to one liquid suction head, the liquid suction head includes a gun head, and the second direction intersects with the first direction and the height direction of the support frame 600.

When the waste removal module 900 removes waste within the reaction chamber 41 of the pre-packaged consumable 40, it is necessary to go through the following steps: 1. the driving assembly 700 drives the support frame 600 to drive the waste liquid removing module 900 to move along the first direction to be opposite to the reaction cavity 41 in the height direction of the support frame 600; 2. the second linear module 910 drives the liquid suction pipe 920 and a gun head (not shown) positioned thereon to descend and insert into the reaction chamber 41 to suck the waste liquid into the waste liquid container; 3. the second linear module 910 drives the suction line 920 and the gun head thereon to be lifted to a corresponding height.

Specifically, the second linear module 910 may be a synchronous belt linear module, a ball screw linear module, a rack and pinion sliding module, or even a cylinder or an electric cylinder, which is not limited herein.

Further, the waste removal module 900 also includes a gun head removing mechanism coupled to the pipetting line 920 and at least partially movable relative to the adapter 921 to disengage the gun head from the adapter 921.

It will be appreciated that in this embodiment, the plurality of connectors 921 on the liquid suction line 920 are detachably connected to a gun head, and only the gun head needs to be replaced after each time the waste liquid is removed. Of course, in other embodiments, the liquid suction head may also use a sampling needle, and the sampling needle may be cleaned after the waste liquid is removed each time in cooperation with a sampling needle cleaning mechanism.

In some embodiments, as shown in fig. 1 and 2, consumable carrier 300 also has a gun head placement location for placement of gun head 50. The gun head placement position is used for placing gun heads 50 for use with the pipetting module 800 and the waste removal module 900.

According to another aspect of the present application, there is also provided a nucleic acid detecting integrated machine, as shown in fig. 9, which includes a PCR detector 20 and the nucleic acid extractor 10 of any of the above embodiments, wherein the PCR detector 20 is disposed on a base plate 100.

The nucleic acid detection integrated machine integrates the nucleic acid extractor 10 and the PCR detector 20, can realize nucleic acid extraction and detection, and simultaneously, has the advantages and benefits brought by the nucleic acid extractor 10 due to the adoption of the nucleic acid extractor 10 in the embodiment, and ensures the detection precision and efficiency of nucleic acid extraction detection.

The technical features of the above embodiments may be arbitrarily combined, and all possible combinations of the technical features in the above embodiments are not described for brevity of description, however, as long as there is no contradiction between the combinations of the technical features, they should be considered as the scope of the description.

The foregoing examples illustrate only a few embodiments of the application, which are described in detail and are not to be construed as limiting the scope of the claims. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the application, which are all within the scope of the application. Accordingly, the scope of the application should be assessed as that of the appended claims.

Claims (10)

1. A nucleic acid extraction instrument, characterized by comprising a bottom plate (100), and a sample carrier (200), a consumable carrier (300), a magnetic suction module (500), a pipetting module (800) and a waste liquid removal module (900) which are arranged on the bottom plate (100);

the sample carrier (200) is used for placing a sample tube (30);

The consumable carrier (300) is used for placing pre-packaging consumable (40), the pre-packaging consumable (40) comprises a consumable body, and a reaction cavity (41) and a reagent cavity which are arranged on the consumable body, wherein the reagent cavity is filled with sample processing reagent;

The magnetic attraction module (500) is positioned below the consumable carrier (300), and the magnetic attraction module (500) is provided with a magnetic attraction piece (520) which can be close to or far away from the reaction cavity (41);

The pipetting module (800) is for transferring liquid between the sample carrier (200) and the consumable carrier (300);

The waste liquid removing module (900) comprises a liquid suction pipeline (920) and a liquid suction head arranged on the liquid suction pipeline (920), wherein the liquid suction pipeline (920) is used for communicating with a waste liquid container, the liquid suction head can move relative to the consumable carrier (300), and waste liquid in the reaction cavity (41) can be sucked to the waste liquid container through negative pressure.

2. The nucleic acid extractor of claim 1, wherein the nucleic acid extractor (10) further comprises a carrier base (400), the carrier base (400) being slidably disposed on the base plate (100), the sample carrier (200), the consumable carrier (300), and the magnetic attraction module (500) being disposed on the carrier base (400).

3. The nucleic acid extractor of claim 1, wherein the magnetic attraction module (500) further comprises a driving mechanism (510) and a heating body (530), the magnetic attraction piece (520) and the heating body (530) are both arranged on the driving mechanism (510), and the magnetic attraction piece (520) and the heating body (530) can be close to or far away from the reaction cavity (41) under the action of the driving mechanism (510).

4. The nucleic acid extractor according to claim 3, wherein the magnetic attraction member (520) and the heating body (530) are arranged side by side, a plurality of pre-packaged consumable materials (40) can be placed side by side on the consumable material carrier (300), a plurality of magnetic attraction grooves (521) are arranged on the magnetic attraction member (520) at intervals, a plurality of heating grooves (531) are arranged on the heating body (530) at intervals, and a plurality of pre-packaged consumable materials (40), a plurality of magnetic attraction grooves (521) and a plurality of heating grooves (531) are respectively in one-to-one correspondence in the arrangement direction of the magnetic attraction member (520) and the heating body (530);

The magnetic attraction piece (520) and the heating body (530) can move in the arrangement direction of the magnetic attraction piece (520) and the heating body (530) and in the height direction of the reaction cavity (41) under the action of the driving mechanism (510).

5. The nucleic acid extraction instrument according to claim 1, wherein a support frame (600) and a driving assembly (700) are disposed on the bottom plate (100), the support frame (600) is slidably disposed on the bottom plate (100) along a first direction, the driving assembly (700) is connected to the support frame (600) and is used for driving the support frame (600) to move in the first direction, the pipetting module (800) and the waste liquid removing module (900) are both disposed on the support frame (600), and the pipetting module (800) and the waste liquid removing module (900) can be respectively opposite to the sample carrier (200) and the consumable carrier (300) in a height direction of the support frame (600) in a process of moving along the first direction along with the support frame (600).

6. The nucleic acid extractor of claim 5, characterized in that the driving assembly (700) is disposed on a side of the bottom plate (100) facing away from the support frame (600), the driving end of the driving assembly (700) is connected with an adapter (750), and the adapter (750) is connected to an end of the support frame (600) near the bottom plate (100).

7. The nucleic acid extraction apparatus according to claim 5, characterized in that the pipetting module (800) comprises a first linear module (810) and a multichannel pipetting assembly (820), the first linear module (810) is arranged on the support frame (600), the multichannel pipetting assembly (820) is arranged on the first linear module (810) and can move in the height direction of the support frame (600) under the action of the first linear module (810), the multichannel pipetting assembly (820) is provided with a plurality of mounting heads (821) for detachably mounting gun heads, the mounting heads (821) are sequentially arranged in a second direction, and the multichannel pipetting assembly (820) can provide a force for sucking or discharging a solution by each gun head, and the second direction intersects with both the first direction and the height direction of the support frame (600).

8. The nucleic acid extraction apparatus according to claim 5, wherein the waste liquid removing module (900) further comprises a second linear module (910), the second linear module (910) is disposed on the support frame (600), the liquid suction pipe (920) is disposed on the second linear module (910) and can move in a height direction of the support frame (600) under the action of the second linear module (910), the liquid suction pipe (920) has a plurality of connectors (921) sequentially disposed in a second direction, each connector (921) is detachably connected with one liquid suction head, the liquid suction head comprises a gun head, and the second direction intersects with the first direction and the height direction of the support frame (600).

9. The nucleic acid extractor according to claim 7 or 8, characterized in that the consumable carrier (300) further has a gun head placement position for placing a gun head (50).

10. A nucleic acid detection all-in-one machine, characterized by comprising a PCR detector (20) and the nucleic acid extractor (10) according to any one of claims 1 to 9, the PCR detector (20) being disposed on the base plate (100).