CN211505220U - Full-automatic multi-wavelength angle excitation fluorescence luminosity detection analyzer - Google Patents
Full-automatic multi-wavelength angle excitation fluorescence luminosity detection analyzer Download PDFInfo
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- CN211505220U CN211505220U CN201921695970.2U CN201921695970U CN211505220U CN 211505220 U CN211505220 U CN 211505220U CN 201921695970 U CN201921695970 U CN 201921695970U CN 211505220 U CN211505220 U CN 211505220U
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Abstract
The utility model discloses a full-automatic multi-wavelength angle excitation fluorescence luminosity detection analysis appearance, which comprises a housin, one side of casing is equipped with LCD, LCD and LCD drive and interface circuit connection, be equipped with sample automatic acquisition transport module in the casing, sample automatic acquisition transport module is connected with fluorescence luminosity detection module, one side of fluorescence luminosity detection module is equipped with excitation light source module, excitation light source module is connected with fluorescence luminosity detection module, and power module is connected with each module, one side of power module is equipped with system control and data analysis processing module. The full-automatic multi-wavelength angle excitation fluorescence photometric detection analyzer realizes automation of processes from collection and conveying of a detection sample to selection of excitation light/emission light wavelength, fluorescence detection, analysis and processing of concentration of a substance to be detected and the like, so that a fluorescence photometric analysis method is more convenient, standard and diversified in high efficiency.
Description
Technical Field
The utility model relates to a trace analysis detects technical field, particularly, relates to a full-automatic multi-wavelength angle arouses fluorescence luminosity detection analysis appearance.
Background
The fluorescence photometry is a method for qualitatively or quantitatively analyzing a substance by using the property that the substance emits a characteristic spectrum of a relatively long wavelength after absorbing light energy of a relatively short wavelength. The composition and structure of different substances are different, the absorbed ultraviolet-visible light wavelength and the emitted light wavelength are also different, the same substance has a specific excitation spectrum and a specific fluorescence spectrum, and the shape and the position of the excitation spectrum and the fluorescence spectrum of an unknown substance can be compared with the spectrum of a standard substance, so that the qualitative analysis can be carried out on the unknown substance. Under the irradiation of exciting light with specific wavelength and strength, the concentration of the matter is different, and the emitted fluorescence strength is different, so that the fluorescence strength of the matter can be measured and quantified. The fluorescence spectrophotometry has higher detection sensitivity and is a better method for detecting and analyzing trace substances. Has wide application in health inspection, environment and food analysis, medicine analysis, biochemistry, clinical detection and other aspects.
Due to the few developed application projects in clinical detection and the like, the automatic development level is low, the specificity is strong, and the technical advantages are far from being fully shown.
SUMMERY OF THE UTILITY MODEL
To the above-mentioned technical problem among the correlation technique, the utility model provides a full-automatic multi-wavelength angle arouses fluorescence luminosity detection analysis appearance can overcome the above-mentioned not enough of prior art.
In order to achieve the technical purpose, the technical scheme of the utility model is realized as follows:
a full-automatic multi-wavelength angle excitation fluorescence luminosity detection analyzer comprises a shell, wherein a liquid crystal display is arranged on one side of the shell and is connected with a liquid crystal display driving and interface circuit, a sample automatic collection and conveying module is arranged in the shell and is connected with a fluorescence luminosity detection module, an excitation light source module is arranged on one side of the fluorescence luminosity detection module and is connected with the fluorescence luminosity detection module, a power supply module is arranged on one side of the excitation light source module and is connected with the sample automatic collection and conveying module, the fluorescence luminosity detection module, the excitation light source module and the liquid crystal display driving and interface circuit, a system control and data analysis processing module is arranged on one side of the power supply module and is respectively connected with the sample automatic collection and conveying module, the, The fluorescence luminosity detection module is in serial port communication connection, a pure water bottle is arranged on one side of the system control and data analysis processing module, and a cleaning liquid bottle is arranged on one side of the pure water bottle;
the automatic sample collecting and conveying module comprises a single-tube sample rack Z-axis linear module, a sampling needle Y-axis linear module is arranged on one side of the single-tube sample rack Z-axis linear module, the sampling needle Y-axis linear module is respectively connected with a plurality of electromagnetic valves through pipelines, the electromagnetic valves are respectively connected with a plunger pump and a waste discharge pump through pipelines, the plunger pump and the waste discharge pump are respectively connected with a flow sensor through the electromagnetic valves and the pipelines, and the single-tube sample rack Z-axis linear module, the sampling needle Y-axis linear module, the plunger pump, the electromagnetic valves, the flow sensor and the waste discharge pump are all connected with a sample collecting and conveying information control processing system;
the fluorescence luminosity detection module comprises an excitation light irradiation light path, an emission light detection light path and a fluorescence luminosity detection information control processing system, wherein an excitation/detection window is arranged at the top end of the emission light detection light path, and a PMT detector is arranged at the tail end of the emission light detection light path; the device comprises an excitation/detection window, a PMT detector, a transmission photoelectric filter wheel, a plano-convex lens II, a plano-convex lens III, a collimating mirror with an optical fiber interface, an excitation photoelectric filter wheel, a plano-convex lens I and a guide groove, wherein the transmission photoelectric filter wheel is arranged between the excitation/detection window and the PMT detector, the plano-convex lens II is arranged between the excitation/detection window and the transmission photoelectric filter wheel, the plano-convex lens III is arranged between the transmission photoelectric filter wheel and the PMT detector, the collimating mirror with the optical fiber interface is arranged at the tail end of an excitation light irradiation light path, the excitation photoelectric filter wheel is arranged between the excitation/detection window and the collimating mirror. The emission photoelectric filter wheel is connected with the first driving motor, the excitation photoelectric filter wheel is connected with the second driving motor, and the first driving motor, the second driving motor and the PMT detector are all connected with the fluorescence luminosity detection information control processing system.
Further, the single-tube sample rack Z-axis (or X-axis) linear module 202 and the Y-axis sampling needle linear module 201 are laterally installed on the same substrate, and after the single-tube sample rack horizontally moves to the sample collection position, the vertical center of the sample tube in the sample rack coincides with the Y-axis vertical movement track of the sampling needle.
Furthermore, be equipped with the flow detection pond on the guide way, the flow detection pond is including detecting the pond, it is connected with liquid inflow end, outflow end respectively to detect the pond, liquid inflow end, outflow end pass through the pipeline with sample automatic acquisition transport module connects.
Further, the diameter of the detection cell is 5-12 mm.
Furthermore, the thickness of the detection cell is 0.1-1 mm.
Further, the emission light path is vertically arranged, and the excitation light path is obliquely arranged.
Furthermore, the sample collecting and conveying information control and processing system and the fluorescence luminosity detection information control and processing system are single-chip microcomputer systems.
Furthermore, the first driving motor and the second driving motor are both stepping motors.
Furthermore, a plurality of filters with different wavelengths are installed in the exciting light electric filter wheel, and the filters are led into the light source through the optical fiber for the optical fiber interface of the collimating mirror.
Further, a plurality of filters with different wavelengths are installed in the transmitting electrokinetic filter wheel.
The utility model has the advantages that: the utility model discloses a full-automatic multi-wavelength angle arouses fluorescence luminosity detection analysis appearance's sample automatic acquisition transport module, fluorescence luminosity detection module mutual independence each other, realize the sample contact through the mobile detection pond, realize the operation flow contact through system control and data analysis processing module (information processing control system), be a real modular structure, the collection from detecting the sample has been realized, carry, to excitation light/emission optical wavelength selection, fluorescence detection, the automation of processes such as the material concentration analysis processing that awaits measuring, it is more convenient to make fluorescence luminosity analytic method obtain, more standard, more manifold high efficiency is used. The automatic sample collecting and conveying module, the fluorescence luminosity detection module and the flow detection pool can be respectively and independently matched with other systems for use to form various products.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
Fig. 1 is a schematic plan view of a fully automatic multi-wavelength angle-excitation fluorescence photometric detection analyzer according to an embodiment of the present invention;
fig. 2 is a schematic structural diagram of an automatic sample collection and conveying module according to an embodiment of the present invention;
FIG. 2-1 is a schematic view of a single-tube sample holder and a sampling needle in a sample placement position;
FIG. 2-2 is a schematic view of a single tube sample holder and sampling needle position at a sample collection site;
fig. 3 is a schematic structural diagram of a flow detection cell according to an embodiment of the present invention;
FIG. 4 is a schematic structural diagram of a fluorescence photometric detection module according to an embodiment of the present invention;
FIG. 4-1 is a schematic view of a fluorescence photometric detection module according to an embodiment of the present invention;
in the figure: 1. an automatic sample collection and conveying module, 2, a flow detection pool, 3, a fluorescence luminosity detection module, 4, an excitation light source module, 5, a power supply module, 6, a system control and data analysis processing module, 7, a pure water bottle, 8, a cleaning liquid bottle, 9, a liquid crystal display driving and interface circuit, 10, a liquid crystal display, 11, a shell, 12, a sample collection and conveying information control and processing system, 201, a sampling needle Y-axis linear module (called a "sampling needle module" for short), 202, a sample rack Z-axis linear module (called a "sample rack module" for short), 203, a flow sensor, 204, a waste discharge pump, 205, a plunger pump, 206, an electromagnetic valve, 211, a sampling needle module lead screw motor, 212, a sampling needle sliding support, 213, a sampling needle, 214, a sampling needle guiding and cleaning support, 215, a sample tube, 216, a single-tube sample rack, 217 and a sample rack guide rail, 218. a sampling needle module guide rail base 219, a sample automatic collection and conveying module substrate 301, a liquid inflow end 302, an outflow end 303, a detection cell 401, an excitation/detection window 402, a plano-convex lens I, a plano-convex lens II, a plano-convex lens 403, an excitation light electric filter wheel 404, a collimating mirror 405, a plano-convex lens III, a PMT detector 406, a PMT detector 407, an emission light electric filter wheel 408, a plano-convex lens II, a plano-convex lens 409, a guide groove 411, an excitation light path inclined circular cavity 412, an excitation light filter wheel cavity 413, an excitation light filter wheel and driving motor mounting table 414, an excitation light filter wheel motor cavity 415, a driving motor II, a 416, an emission light path vertical circular cavity 417, an emission light filter wheel cavity 418, an emission light filter wheel and driving motor mounting table 419, an emission light filter wheel motor cavity 420 and a driving motor I.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art all belong to the protection scope of the present invention.
As shown in fig. 1-4, according to the embodiment of the present invention, the full-automatic multi-wavelength angle-excited fluorescence luminosity detection analyzer comprises a housing 11, one side of the housing 11 is provided with a liquid crystal display 10, the liquid crystal display 10 is connected with a liquid crystal display driver and an interface circuit 9, a sample automatic collection and transportation module 1 is arranged in the housing 11, the sample automatic collection and transportation module 1 is connected with a fluorescence luminosity detection module 3 (i.e. an automatic multi-wavelength angle-excited fluorescence luminosity detection module), one side of the fluorescence luminosity detection module 3 is provided with an excitation light source module 4, the excitation light source module 4 is connected with the fluorescence luminosity detection module 3, one side of the excitation light source module 4 is provided with a power supply module 5, the power supply module 5 is connected with the sample automatic collection and transportation module 1, the fluorescence luminosity detection module 3, the excitation light, The liquid crystal display driving and interface circuit 9 is connected, a system control and data analysis processing module 6 is arranged on one side of the power supply module 5, the system control and data analysis processing module 6 is respectively in serial port communication connection with the sample automatic acquisition and conveying module 1 and the fluorescence luminosity detection module 3, a pure water bottle 7 is arranged on one side of the system control and data analysis processing module 6, and a cleaning liquid bottle 8 is arranged on one side of the pure water bottle 7. The full-automatic multi-wavelength angle excitation fluorescence luminosity detection analyzer is controlled by a system, automatically collects and transmits a preprocessed detected sample to a fluorescence luminosity detection window, and automatically selects corresponding excitation light wavelength and emission light wavelength to perform fluorescence luminosity detection analysis according to the requirement of a substance to be detected in the detected sample.
The automatic sample collecting and conveying module 1 comprises a single-tube sample rack Z-axis (or X-axis) linear module 202, a sampling needle Y-axis linear module 201 is arranged above one side of the single-tube sample rack Z-axis linear module 202, the single-tube sample rack Z-axis linear module 202 and the sampling needle Y-axis linear module 201 are driven by stepping motors, sampling needles 213 are respectively connected with a plurality of electromagnetic valves 206 through pipelines, the electromagnetic valves 206 are respectively connected with a plunger pump 205 and a waste discharge pump 204 through pipelines, the plunger pump 205 and the waste discharge pump 204 are respectively connected with a flow sensor 203 through the electromagnetic valves 206 and the pipelines, and the single-tube sample rack Z-axis linear module 202, the sampling needle Y-axis linear module 201, the plunger pump 205, the electromagnetic valves 206, the flow sensor 203 and the waste discharge pump 204 are all connected with a sample collecting and conveying information control processing system 12. The plunger pump 205 is used to deliver a liquid.
The sampling needle Y-axis linear module 201 drives a sampling needle sliding support 212 by a sampling needle module screw rod motor 211, makes Y-axis linear movement along a sampling needle module guide rail base 218, and leads the sampling needle to a cleaning support 214 through the sampling needle guide, so that a stainless steel sampling needle 213 only moves linearly in the Y-axis direction; in the embodiment, a motor arranged on the inner side of a substrate 219 of an automatic sample collection and conveying module is used for driving a Z-axis (or X-axis) linear module 202 of a single-tube sample rack through gear and rack transmission, namely, a rack arranged on the inner side of a single-tube sample rack 216 is driven through a gear, the rotary motion of a motor is changed into the linear motion of the sample rack, and the linear motion of the sample rack in the Z-axis (or X-axis) direction is realized through the guide of a sample rack guide rail 217; the sampling needle Y-axis linear module 201 and the single-tube sample rack Z-axis linear module 202 are both arranged on the outer side of the sample automatic acquisition and conveying module substrate 219, and after the single-tube sample rack Z-axis linear module 202 moves to a sample acquisition position (shown in figure 2-2), the vertical center of a sample tube 215 placed in the sample rack 216 coincides with the Y-axis vertical movement track of the sampling needle 213.
The automatic sample collecting and conveying module 1 completes the necessary functions of collecting and conveying samples, cleaning the inside and outside of a sampling needle, cleaning a flow detection pool and a sample conveying pipeline, discharging waste liquid and other liquid samples under the instruction of a sample collecting and conveying information control processing system 12, and the main working process is as follows: the user (or the automatic manipulator) is immediately off the machine after the sample tube 215 is placed/replaced in the sample frame of the sample taking and placing position (figure 2-1), the motor drives the sample frame to move linearly to the sample collecting position (figure 2-2) right below the sampling needle, the Y-axis linear module drives the sampling needle 213 to suck a sample into the sample tube 215, the collected sample is automatically conveyed to the flow detection pool, the detection module is informed to detect the sample, and then the sampling needle and the sample frame are reset to the sample taking and placing position, and the sampling needle, the flow detection pool and the sample conveying pipeline are automatically cleaned.
In addition, the automatic sample collecting and conveying module has a strong fault self-diagnosis function. The sample frame moving mechanism, the sampling needle linear module and the plunger pump are all provided with position sensors, so that the moving parts can be monitored in real time; the liquid level sensors such as cleaning and waste liquid are equipped, and the relevant liquid level conditions are monitored in real time: insufficient liquid such as cleaning liquid and the like or excessive waste liquid; the flow sensor 203 is arranged to monitor the functional state of the liquid path in real time, and can indirectly detect the related components (such as an electromagnetic valve, a waste discharge pump and the like) of liquid conveying. When some fault is detected, the module gives an alarm and suspends the operation until the fault is treated and corrected.
Different from the sample conveying and collecting mechanism of a common analysis instrument, the embodiment provides a unitized design in which the single-tube sample rack Z-axis (or X-axis) linear module 202 and the Y-axis sampling needle linear module 201 are laterally installed on the sample automatic collecting and conveying module substrate 219, so that the sample collecting mechanism of the sample automatic collecting and conveying module has better mechanical stability, flexibility and convenience. Not only can use an open sample tube, but also can puncture and sample directly from a closed sample tube with a puncture cap, and is safe and convenient. Can be used as a basic structure for automatically collecting various liquid samples. The matching of the Z-axis (or X-axis) movable sample rack and the Y-axis sampling needle linear module can be one-to-one matching, also can be one-to-two or one-to-three matching, and the detection analysis products with different detection items, methods or functions can be conveniently integrated into an integrated full-automatic multifunctional detection analysis product as long as the detection samples are the same.
The automatic sample collecting and conveying module has a key-operation-free self-starting function: the sample tube in-place detector is arranged, and the module automatically starts to work after a new sample tube is put on the Z-axis (or X-axis) moving sample rack. The design provides a simple and reliable modularized matching for a higher-level full-automatic detection and analysis system which realizes multi-sample complex analysis by taking and placing samples from a full-automatic sample pretreatment module through a three-dimensional manipulator.
The sample collection and transmission information control processing system is a single chip microcomputer system with independent and perfect functions, and can independently realize the functions of the sample automatic collection and transmission module, and the sample automatic collection and transmission module is provided with a level signal control interface for the detection module except a reserved 485/232 communication interface: after the sample is collected and conveyed in place, the high level is output to inform the detection module to start working, the work is finished, the detection module outputs the high level, and the module finishes waste discharge, cleaning, self-checking and other works immediately. Different detection modules can be simply matched without software matching.
The fluorescence luminosity detection module 3 comprises an excitation light irradiation light path, an emission light detection light path and a fluorescence luminosity detection information control processing system which are arranged on the same side of the circular excitation/detection window 401, wherein the emission light path is vertically arranged, and the excitation light path is obliquely arranged. The circular excitation/detection window 401 is vertically concentric to the top of the emission light detection optical path, at the end of which a PMT (photomultiplier tube) detector 406 is disposed; the excitation/detection window 401 with install between the PMT detector 406 and emit light electronic filter wheel 407, excitation/detection window 401 with install planoconvex lens two 408 between the electronic filter wheel 407 of emitting light, emit light electronic filter wheel 407 with install planoconvex lens three 405 between the PMT detector 406, the collimating mirror 404 of taking the fiber interface is installed to the end that the excitation light shines the light path, excitation/detection window 401 with be equipped with excitation light electronic filter wheel 403 between the collimating mirror 404 of taking the fiber interface, excitation light electronic filter wheel 403 with be equipped with planoconvex lens 402 between the excitation/detection window 401, be equipped with guide way 409 on the excitation/detection window 401.
The fluorescence luminosity detection module 3 adopts an integrated 6-cavity structure (as shown in figure 4-1), and except for a light source, all optical devices, filter wheels, a driving mechanism and a fluorescence luminosity detection information control processing system are respectively assembled in different cavity grids. The fixed optical device of the emitted light path is arranged in a vertical round cavity 416 right below the excitation/detection window, the fixed optical device of the excitation light path is arranged in an inclined round cavity 411 below the side of the excitation/detection window, and 2 round cavities are arranged on the same side of the excitation/detection window; an optical filter switching groove is formed in the middle of the inclined circular cavity 411, the excitation light filter wheel and a driving mechanism thereof are installed on an installation surface 413 perpendicular to the circular cavity 411 and are divided into an upper cavity lattice and a lower cavity lattice through the installation surface 413, the upper cavity lattice and the lower cavity lattice are 412 and 414 respectively, the optical filter wheel is installed in the cavity lattice 412, a motor 415 is installed in the cavity lattice 414, and an optical filter in the excitation light filter wheel is driven by the motor 415 to be automatically switched into an optical path of the circular cavity 411; the middle part of the vertical round cavity 416 is provided with a filter switching groove, the emission light filter wheel and a driving mechanism thereof are arranged on an installation surface 418 which is vertical to the round cavity 416, the emission light filter wheel is divided into an upper cavity grid and a lower cavity grid which are 417 and 419 respectively through the installation surface 418, the filter wheel is arranged in the cavity grid 417, a motor 420 is arranged in the cavity grid 419, and the filter in the emission light filter wheel is driven by the motor 420 to be automatically switched into the light path of the round cavity 416; the information control processing and detection circuitry is mounted in a housing 419 that is isolated from the optics. The structure design is easier to realize the modularization of the optical detection functional unit, and the stray light is easier to eliminate.
The excitation light electric filter wheel and the emission light electric filter wheel are driven by a stepping motor, a plurality of optical filters with different wavelengths are pre-installed in the 2 optical filter wheels, and the 2 optical filter wheels respectively rotate to appointed switching positions under the instruction control of a fluorescence luminosity detection information control processing system, so that the combined selection of different excitation light and emission light wavelengths is realized.
The light source is led into a collimating mirror through an optical fiber for collimation, after the single color of the optical filter is selected by an exciting light electric optical filter wheel 403, the light is converged into parallel light through a plano-convex lens I402, and the parallel light is incident to a sample to be detected (not shown in figure 4) in a sample detection container on an exciting/detecting window 401 at a certain angle. Under the instruction of a fluorescence luminosity detection information control processing system (not shown in figure 4), after a sample to be detected is irradiated by excitation light with specific wavelength and certain light intensity, emission light with specific wavelength different from the excitation light is generated, the emission light intensity is highly related to the concentration of characteristic substances in the sample to be detected, and under the condition of certain light source intensity, trace detection on different substances can be conveniently, quickly and accurately realized through the combined selection of different excitation light and emission light wavelengths.
The optical fiber interface collimating lens is used at the input end of the light source, the selection and the allocation of the light source are very flexible, even the combination of various light sources such as a bandwidth light source and a grating monochromatic light source can be realized, and the selection of the wavelength of the exciting light breaks through the limit of the number of pre-installed optical filters in the optical filter wheel. The fluorescence luminosity detection module can also realize chemiluminescence sample detection by presetting a light shielding sheet in the excitation light filter wheel and presetting a wide-band light-passing sheet combination in the emission light filter wheel.
The guide slot 409 can be selected by the application of the class 2 detection container: one is a fixed type, that is, the detection container (such as the aforementioned flow detection cell 2) is fixed and positioned to the excitation/detection window through the guide groove, and the sample in the detection container is replaced in a liquid flow conveying manner; the other type is a mobile type, and a movable detection container (such as a customized glass slide, a detection board card and the like) carrying a sample is movably placed on an excitation/detection window through a mechanism matched with a guide groove and the detection container. In any case, the center of the sample detection area of the detection container coincides with the center of the detection window.
The flow detection pool 2 is a detection container specially matched with automatic collection and conveying (distribution) of a liquid sample, the flow detection pool 2 comprises a detection pool 303, the detection pool 303 is respectively connected with a liquid inflow end 301 and a liquid outflow end 302, and the liquid inflow end 301 and the liquid outflow end 302 are respectively connected with the automatic collection and conveying module 1 of the sample through pipelines.
The flow detection cell 2 is made of quartz glass, and is only limited by double-sided light transmission of the detection cell, the carved detection cell 303 is applicable to both the diameter of 5-12mm and the thickness of 0.1-1mm, the design gives consideration to the requirements of miniaturization, low sample volume and high sensitivity of trace substance detection of an optical detection system, the requirements of selection and processing of devices of a sampling module and a detection module can be met under the condition of high precision, and the whole system can be well balanced between high performance and relatively low manufacturing cost and use cost. The flow detection cell with the design is suitable for detection containers of various low-viscosity liquid samples, is not limited to be applied to the device, and can also be used in other devices for luminescence analysis, scattered light analysis, transmitted light detection, image analysis and the like.
The PMT detector can also be replaced by a detection unit taking a CCD image sensor as a core, and the application field of the fluorescence luminosity detection module can be further expanded. If only the fluorescence photometric detection is considered, the structure that the emission light path is vertical and the excitation light path is inclined on the same side below the excitation/detection window is only one of the preferred structures, and the structure can also be a light path structure with other angles on the same side below the excitation/detection window.
The sample collection, transmission, information control and processing system 12 and the fluorescence luminosity detection information control and processing system are single-chip microcomputer systems with independent and complete functions, the functions of the modules can be independently realized, information exchange, instruction receiving or detection data uploading are realized through 485/232 communication serial ports, the system control and data analysis and processing module 6 coordinates operation flows among the modules, and a sample is collected and transmitted into a flow detection pool on a fluorescence luminosity detection window, so that detection and analysis of different substances to be detected are fully automatically completed by a fluorescence luminosity method. To sum up, with the aid of the above technical scheme, the utility model discloses a full-automatic multi-wavelength angle arouses fluorescence luminosity detection analysis appearance's sample automatic acquisition transport module, fluorescence luminosity detection module mutual independence each other, realize the sample contact through the flow detection pond, realize the operation flow contact through information processing control system, are a real modular structure, and sample automatic acquisition transport module, fluorescence luminosity detection module, flow detection pond all can be independent separately and other system coordination use, form the concrete product of multiple different. On one hand, the automation and standardization degree of the fluorescence photometry detection is greatly improved, on the other hand, various different substances are conveniently measured on the same instrument through different exciting light/emitting light wavelength combinations, and the popularization of the fluorescence photometry is facilitated.
The above description is only a preferred embodiment of the present invention, and should not be taken as limiting the invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A full-automatic multi-wavelength angle excitation fluorescence luminosity detection analyzer is characterized by comprising a shell (11), wherein one side of the shell (11) is provided with a liquid crystal display (10), the liquid crystal display (10) is connected with a liquid crystal display driving and interface circuit (9), a sample automatic acquisition and conveying module (1) is arranged in the shell (11), the sample automatic acquisition and conveying module (1) is connected with a fluorescence luminosity detection module (3), one side of the fluorescence luminosity detection module (3) is provided with an excitation light source module (4), the excitation light source module (4) is connected with the fluorescence luminosity detection module (3) through an optical fiber, a power supply module (5) is connected with the sample automatic acquisition and conveying module (1), the fluorescence luminosity detection module (3), the excitation light source module (4) and the liquid crystal display driving and interface circuit (9), a system control and data analysis processing module (6) is arranged on one side of the power supply module (5), the system control and data analysis processing module (6) is respectively in serial port communication connection with the automatic sample collection and conveying module (1) and the fluorescence luminosity detection module (3), a pure water bottle (7) is arranged on one side of the system control and data analysis processing module (6), and a cleaning liquid bottle (8) is arranged on one side of the pure water bottle (7);
the automatic sample collecting and conveying module (1) comprises a single-tube sample rack Z-axis linear module (202), a sampling needle Y-axis linear module (201) is arranged on one side of the single-tube sample rack Z-axis linear module (202), the sampling needle Y-axis linear module (201) is connected with a plurality of electromagnetic valves (206) through pipelines respectively, the electromagnetic valves (206) are connected with a plunger pump (205) and a waste discharge pump (204) through pipelines respectively, the plunger pump (205) and the waste discharge pump (204) are connected with a flow sensor (203) through the electromagnetic valves (206) and the pipelines respectively, and the single-tube sample rack Z-axis linear module (202), the sampling needle Y-axis linear module (201), the plunger pump (205), the electromagnetic valves (206), the flow sensor (203) and the waste discharge pump (204) are connected with a sample collecting and conveying information control processing system (12);
the fluorescence luminosity detection module (3) comprises an excitation light irradiation light path, an emission light detection light path and a fluorescence luminosity detection information control processing system, wherein an excitation/detection window (401) is arranged at the top end of the emission light detection light path, and a PMT detector (406) is arranged at the tail end of the emission light detection light path; a light emitting electro-kinetic filter wheel (407) is mounted between the excitation/detection window (401) and the PMT detector (406), a plano-convex lens II (408) is arranged between the excitation/detection window (401) and the emitted light electrodynamic filter wheel (407), a plano-convex lens III (405) is arranged between the emission light electro-dynamic filter wheel (407) and the PMT detector (406), the tail end of the excitation light irradiation light path is provided with a collimating lens (404) with an optical fiber interface, an excitation light electric filter wheel (403) is arranged between the excitation/detection window (401) and the collimating mirror (404) with the optical fiber interface, a plano-convex lens I (402) is arranged between the excitation light electric filter wheel (403) and the excitation/detection window (401), a guide groove (409) is arranged on the excitation/detection window (401); the emission light electric filter wheel (407) is connected with a first driving motor (420), the excitation light electric filter wheel (403) is connected with a second driving motor (415), and the first driving motor (420), the second driving motor (415) and the PMT detector (406) are all connected with the fluorescence luminosity detection information control processing system.
2. The fully automatic multi-wavelength angle-excitation fluorescence photometric detection analyzer according to claim 1 wherein the single tube sample holder Z-axis linear module 202 and the Y-axis sampling needle linear module 201 are mounted on the sample automatic collection and transport module substrate (219), and after the single tube sample holder moves horizontally to the sample collection position, the vertical center of the sample tube in the sample holder coincides with the Y-axis vertical movement track of the sampling needle.
3. The full-automatic multi-wavelength angle-excitation fluorescence photometric detection analyzer according to claim 1, wherein the guide groove (409) is provided with a flow detection cell (2), the flow detection cell (2) comprises a detection cell (303), the detection cell (303) is respectively connected to the liquid inflow end (301) and the liquid outflow end (302), and the liquid inflow end (301) and the liquid outflow end (302) are respectively connected to the sample automatic collection and transportation module (1) through a pipeline.
4. The fully automated multi-wavelength angle-excitation fluorometric detection analyzer of claim 3, wherein the diameter of the detection cell (303) is 5-12mm and the thickness of the detection cell (303) is 0.1-1 mm.
5. The fully automated multi-wavelength angle-excitation fluorometric detection analyzer of claim 1, wherein the emission light detection optical path is vertically disposed and the excitation light illumination optical path is obliquely disposed.
6. The fully automatic multi-wavelength angle-excitation fluorescence photometric detection analyzer according to claim 1 wherein the sample collection and transportation information control processing system (12) and the fluorescence photometric detection information control processing system are single chip microcomputer systems.
7. The fully automatic multi-wavelength angle-excitation fluorophotometric detection analyzer according to claim 1, wherein the first drive motor (420) and the second drive motor (415) are both stepper motors.
8. The full-automatic multi-wavelength angle-excitation fluorescence photometric detection analyzer according to claim 1, wherein a plurality of filters of different wavelengths are installed in the excitation electro-optical filter wheel (403), and are guided to the light source by an optical fiber through an optical fiber interface of the collimating mirror.
9. The fully automatic multi-wavelength angle-excitation fluorometric detection analyzer of claim 1, wherein a plurality of filters of different wavelengths are mounted in the emission light electro-dynamic filter wheel (407).
10. The fully automatic multi-wavelength angle-excitation fluorescence photometric detection analyzer according to claim 1 wherein the fluorescence photometric detection module (3) employs an integrated 6-cell structure for assembling optics, excitation light motorized filter wheel (403), emission light motorized filter wheel (407), driving motor one (420), driving motor two (415), and fluorescence photometric detection information control processing system.
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| CN201921695970.2U CN211505220U (en) | 2019-10-11 | 2019-10-11 | Full-automatic multi-wavelength angle excitation fluorescence luminosity detection analyzer |
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| CN110726704A (en) * | 2019-10-11 | 2020-01-24 | 北京茂泽科技有限公司 | Full-automatic multi-wavelength angle excitation fluorescence luminosity detection analyzer |
| CN110726704B (en) * | 2019-10-11 | 2024-04-12 | 北京茂泽科技有限公司 | Full-automatic multi-wavelength angle excitation fluorescence photometry detection analyzer |
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