CN106896050B - Blood cell counting plate - Google Patents
Blood cell counting plate Download PDFInfo
- Publication number
- CN106896050B CN106896050B CN201710228099.4A CN201710228099A CN106896050B CN 106896050 B CN106896050 B CN 106896050B CN 201710228099 A CN201710228099 A CN 201710228099A CN 106896050 B CN106896050 B CN 106896050B
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- China
- Prior art keywords
- liquid
- counting
- plate
- sample liquid
- separation sheet
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The patent discloses a blood cell counting plate in the field of biological equipment; the counting device comprises a bottom plate and a cover plate, wherein a counting pool is arranged on the upper surface of the bottom plate; the bottom plate is also provided with a liquid separation sheet which can move on the upper surface of the bottom plate. According to the scheme, the liquid separation sheet is arranged, when the liquid separation sheet is used, redundant sample liquid in the counting tank can be pushed out of the counting tank, so that the actual volume of the sample liquid in the counting tank is consistent with the volume of the counting tank, the condition that the actual volume of the sample liquid in the counting tank is larger than or smaller than the volume of the counting tank is avoided, and the counting precision of the sample liquid is improved.
Description
Technical Field
The application relates to the field of biological equipment, in particular to a blood cell counting plate.
Background
The blood cell counting plate is used for microscopic counting of red and white blood cells in human body, and is also commonly used for counting the number of microorganisms such as bacteria, fungi, yeast and the like, and is a common biological tool. Especially in the disease diagnosis and treatment process, the blood cell counting plate is often required to detect the cells in the blood of a patient, and important basis can be provided for the diagnosis or the observation of the diagnosis and treatment effect of clinical diseases through counting and classifying the cells.
The currently commonly used blood cell counting plates are a Niu Bao counting plate, a Fast-read10 quantitative sediment analysis plate and the like, and the Fast-read10 quantitative sediment analysis plate is taken as an example, wherein 10 large square grids are arranged on the counting plate, the volume of each large square grid is 0.1 mu l, and each plate can be applied to cell counting of 10 specimens. However, when the number of cells is large, the number of cells in the large square is difficult for the worker to count, and the result deviation is large. Based on the situation, the improvement of the Fast-read10 quantitative sediment analysis plate is mainly to divide each large square into 9 middle squares uniformly, divide the middle square in each large square into 9 small squares, count the cells in the 9 small squares under a high-power mirror, and further calculate the number of cells in each liter of sample liquid.
However, in the process of injecting the sample liquid into the square lattice on the counting plate, because the injection mode mainly adopts manual instillation, the instilled sample liquid always overflows the square lattice or does not fill the square lattice, and the sample liquid is calculated according to the volume of each large square lattice during counting, so that the counting of cells in the sample liquid is deviated, the diagnosis of diseases is wrong, and the health of patients is seriously harmed.
Disclosure of Invention
The present application is directed to a blood cell counting plate, and solves the problem of deviation in cell count caused by uncertainty in the injection amount of sample liquid in the square of the counting plate in the prior art.
The blood cell counting plate comprises a bottom plate and a cover plate, wherein a counting pool is arranged on the upper surface of the bottom plate; the bottom plate is also provided with a liquid separation sheet which can move on the upper surface of the bottom plate.
The working principle of the scheme and the beneficial effects thereof are that: the bottom plate is provided with a liquid separation sheet which can move on the upper surface of the bottom plate; when in use, after the sample liquid to be detected is injected into the counting pool on the bottom plate, the sample liquid overflows for counting Chi Shaoxu; then make the separating plate remove on the bottom plate, at separating plate removal in-process, the separating plate promotes unnecessary sample liquid in the counting cell and moves forward, until outside pushing out the counting cell with unnecessary sample liquid to guaranteed that the sample liquid volume in the counting cell is certain, avoided the actual volume of sample liquid in the counting cell to be greater than or less than the condition of counting cell volume, thereby guaranteed the accuracy of sample liquid cell count. When counting, the cover plate is placed above the counting pool, and the microscope is used for counting cells, so that the microscope is prevented from being in direct contact with the sample liquid due to the blocking of the cover plate, and the sanitation and the detection accuracy of the microscope are ensured.
Further, a plurality of counting tanks are arranged. Through setting up a plurality of counting ponds, can detect a plurality of different grade type sample liquid on same blood cell count board to the detection efficiency of sample liquid has been improved.
Furthermore, crisscross liquid guide grooves are arranged between the adjacent counting tanks. Through setting up the drain groove for unnecessary sample liquid that the separation liquid piece was released can be through the drain groove discharge, avoids sample liquid to pile up on the bottom plate, causes the pollution.
Further, the cover plate is provided with a buckling edge, and buckling grooves matched with the buckling edge are formed in the periphery of the counting pool. The cover plate is provided with the buckling edge and is matched with the buckling groove, and when cell counting is carried out, the buckling edge is tightly matched with the buckling groove, so that the cover plate is tightly attached to the upper part of the counting pool, shaking of the cover plate is avoided, and the counting accuracy is improved.
Further, the buckling groove is communicated with the liquid guide groove. The catching groove is communicated with the liquid guide groove, and in the pushing process of the liquid separation sheet, redundant sample liquid is pushed out of the counting tank by the liquid separation sheet, flows into the catching groove, and flows out of the blood cell counting plate through the liquid guide groove communicated with the catching groove, so that the flow of the redundant sample liquid on the upper end face of the bottom plate is reduced, and the cleaning of the counting plate is facilitated.
Further, through holes are formed in the liquid separation sheets. Through the arrangement of the through holes, sample liquid can be injected into the counting tank through the through holes, and as the diameter of the through holes is much smaller than that of the counting tank, the liquid separation sheet is tightly attached to the bottom plate, and after the counting tank is filled with the sample liquid injected into the counting tank, the sample liquid can be quickly found through the through holes, so that the waste of the sample liquid is avoided; and the content of redundant sample liquid in the counting tank is reduced, so that the counting plate is kept clean.
Further, the side wall of the liquid separation sheet is provided with a convex rib extending upwards. After the liquid separating sheet pushes the excessive sample liquid in the counting pool to flow out, when the excessive sample liquid is excessive, the sample liquid is accumulated at the front end of the liquid separating sheet and is higher than the upper end surface of the liquid separating sheet, and the convex edges are arranged, so that the sample liquid is difficult to rise above the liquid separating sheet and always flows in front of the liquid separating sheet; thereby avoiding the upper end surface of the liquid separation sheet from being polluted.
Further, the side wall of the liquid separation sheet is provided with a liquid absorption sheet, and the bottom surface of the liquid absorption sheet is higher than the bottom surface of the liquid separation sheet. Through setting up the imbibition piece, when the excessive sample liquid of spacer bar promotion flows, when the sample liquid in spacer bar place ahead piles up a certain amount, the imbibition piece absorbs unnecessary sample liquid, makes sample liquid content reduce to reduce the pollution of sample liquid to the bottom plate. The bottom surface of the liquid absorption sheet is higher than the bottom surface of the liquid separation sheet, so that the bottom surface of the liquid absorption sheet is always higher than the counting tank, the situation that the liquid absorption sheet absorbs the sample liquid in the counting tank is avoided, the sample liquid in the counting tank is ensured to be certain, and the counting precision of the sample liquid is improved.
Drawings
FIG. 1 is a schematic diagram of a blood cell counting plate according to the present application;
FIG. 2 is a schematic diagram of another embodiment of a blood cell count plate according to the present application.
Detailed Description
The application is described in further detail below by way of specific embodiments:
reference numerals in the drawings of the specification include: the liquid-absorbing device comprises a bottom plate 1, a counting cell 2, a counting grid 3, a liquid guide groove 4, a buckling groove 5, a supporting column 6, a connecting rod 7, a liquid-separating sheet 8, a through hole 9, a convex rib 10, a liquid-absorbing sheet 11 and a gasket 12.
Example 1:
the blood cell counting plate shown in figure 1 comprises a bottom plate 1 and a cover plate with a buckling edge, wherein the upper surface of the bottom plate 1 is provided with four counting cells 2, and the bottom plate 1 is provided with buckling grooves 5 which are matched with the buckling edge and encircle the counting cells 2; each counting cell 2 is equally divided into eight counting cells 3 with the volume of 0.1 mm; two liquid guide tanks 4 which are crisscrossed are arranged between the adjacent counting tanks 2, wherein one liquid guide tank 4 penetrates through two ends of the bottom plate 1; the liquid guide groove 4 is communicated with the buckling groove 5, and the depth of the liquid guide groove 4 is larger than that of the buckling groove 5; the liquid guide groove 4 is connected with a support column 6 in a sliding way, and the support column 6 is formed by
The L-shaped first support column and the L-shaped second support column are formed; the two sides of the support column 6 are connected with telescopic connecting rods 7, the other ends of the connecting rods 7 are connected with liquid separation sheets 8, and the lower surfaces of the liquid separation sheets 8 are attached to the upper surface of the bottom plate 1; the liquid separation sheet 8 is provided with 6 through holes 9; one side of the liquid separation sheet 8, which is close to the counting cell 2, and the other side of the liquid separation sheet are provided with convex ribs 10 which extend upwards; the ribs 10 are provided with liquid absorbing sheets 11, and the bottom surfaces of the liquid absorbing sheets 11 are higher than the bottom surfaces of the liquid separating sheets 8.
During the use, remove support column 6, make the through-hole 9 on the support column 6 be located the top of count check 3, pour into the sample liquid into in the count check 3 through-hole 9, after the sample liquid fills up count pond 2, the sample liquid can gather in through-hole 9, stop the injection of sample liquid this moment, promote support column 6, support column 6 removes and can promote unnecessary sample liquid in the count pond 2 forward, owing to be equipped with bead 10, unnecessary sample liquid can't enter into the upper surface of separating liquid piece 8, liquid absorbing piece 11 also can absorb the sample liquid rather than the contact simultaneously, thereby unnecessary sample liquid on the reduction bottom plate 1 keeps the cleanness of counter plate. Meanwhile, the sample liquid which is not absorbed by the liquid absorbing sheet 11 flows out of the counting plate through the buckling groove 5 and the liquid guiding groove 4; after the liquid separation sheet 8 is pushed on the bottom plate 1, the redundant sample liquid in each counting grid 3 is pushed away by the liquid separation sheet 8, so that the sample liquid volume in each counting grid 3 is ensured to be 0.1 mm; then the cover plate is buckled in the buckling groove 5, and a microscope is used for counting cells in the sample liquid, so that the technical precision is ensured. Meanwhile, as the connecting rod 7 is telescopic, the liquid separation sheet 8 can extend out of the bottom plate 1 for cleaning through extending the supporting rod; is convenient for the next use and avoids pollution.
Example 2:
a blood cell counting plate as shown in fig. 2 is different from the embodiment 1 in that two liquid guiding tanks 4 penetrate through two ends of a bottom plate 1, a supporting column 6 is a cylinder, and one end of the supporting column 6 is connected to the junction of the two liquid guiding tanks 4; the connecting rod 7 is rotatably connected to the supporting column 6, and a gasket 12 is arranged between the connecting rod 7 and the bottom plate 1; the liquid separation sheet 8 is in a long strip shape, and the length of the liquid separation sheet 8 is longer than the longest diagonal line of the counting cell 2.
The counter plates in the embodiment 1 and the embodiment 2 can be increased or decreased according to the actual requirements when being manufactured, and the setting of the counter pool 2 and the counter grid 3 can be increased or decreased; the volume of the counter 3 may also be varied.
The difference between the blood cell count plate of example 2 and example 1 is that the spacer 8 needs to be rotated when the excess sample solution is removed by the spacer of example 2.
The foregoing is merely exemplary embodiments of the present application, and specific structures and features that are well known in the art are not described in detail herein. It should be noted that modifications and improvements can be made by those skilled in the art without departing from the structure of the present application, and these should also be considered as the scope of the present application, which does not affect the effect of the implementation of the present application and the utility of the patent. The protection scope of the present application is subject to the content of the claims, and the description of the specific embodiments and the like in the specification can be used for explaining the content of the claims.
Claims (2)
1. A blood cell counting plate comprises a bottom plate and a cover plate, wherein a counting pool is arranged on the upper surface of the bottom plate; the device is characterized in that the bottom plate is also provided with a liquid separation sheet which can move on the upper surface of the bottom plate; the counting pond is provided with a plurality of counting ponds; a crisscrossed liquid guide groove is arranged between the adjacent counting tanks; the cover plate is provided with a buckling edge, and buckling grooves matched with the buckling edge are formed in the periphery of the counting pool; the buckling groove is communicated with the liquid guide groove; the liquid separation sheet is provided with a through hole; the liquid guide groove is slidably connected with a support column, and the support column consists of an L-shaped first support column and a L-shaped second support column; the two sides of the support column are connected with telescopic connecting rods, the liquid separation sheet is connected with the other ends of the connecting rods, and the lower surface of the liquid separation sheet is attached to the upper surface of the bottom plate; one side of the liquid separation sheet, which is close to the counting tank, and the other side of the liquid separation sheet are provided with convex edges extending upwards; when the liquid-proof plate is used, the support column is moved, the through hole on the liquid-proof plate is positioned above the counting pool, sample liquid is injected into the counting pool through the through hole, after the counting pool is filled with the sample liquid, the sample liquid can be gathered in the through hole, the injection of the sample liquid is stopped at the moment, the support column is pushed, the support column is moved to push the redundant sample liquid in the counting pool forwards, and the redundant sample liquid cannot enter the upper surface of the liquid-proof plate due to the convex edges, so that the redundant sample liquid on the bottom plate is reduced, and the redundant sample liquid pushed away by the liquid-proof plate flows out of the counting plate through the buckling grooves and the liquid guide groove; after the liquid separation sheet is pushed on the bottom plate, the redundant sample liquid in the counting grid is pushed away by the liquid separation sheet, so that the sample liquid volume in each counting cell is ensured to be consistent.
2. A blood cell count plate according to claim 1 wherein: the side wall of the liquid separation sheet is provided with a liquid absorption sheet, and the bottom surface of the liquid absorption sheet is higher than the bottom surface of the liquid separation sheet.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710228099.4A CN106896050B (en) | 2017-04-10 | 2017-04-10 | Blood cell counting plate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710228099.4A CN106896050B (en) | 2017-04-10 | 2017-04-10 | Blood cell counting plate |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106896050A CN106896050A (en) | 2017-06-27 |
| CN106896050B true CN106896050B (en) | 2023-08-29 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710228099.4A Active CN106896050B (en) | 2017-04-10 | 2017-04-10 | Blood cell counting plate |
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| CN (1) | CN106896050B (en) |
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| WO2001074490A2 (en) * | 2000-04-03 | 2001-10-11 | Ecole Polytechnique Federale De Lausanne (Epfl) | A device for dispensing accurately-controlled small doses of liquid |
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| US4065000A (en) * | 1976-06-03 | 1977-12-27 | Gary Murton | Pharmaceutical tablet and capsule counter |
| US4726237A (en) * | 1985-06-19 | 1988-02-23 | Sequoia-Turner Corporation | Fluid metering apparatus and method |
| CN2175433Y (en) * | 1993-11-09 | 1994-08-24 | 金华钧 | Counting means for microscope |
| US5876604A (en) * | 1996-10-24 | 1999-03-02 | Compact Membrane Systems, Inc | Method of gasifying or degasifying a liquid |
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| CN2478109Y (en) * | 2001-03-09 | 2002-02-20 | 赵翀 | Immunity pick-up plate |
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| CN106896050A (en) | 2017-06-27 |
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