CN2775659Y - Plastic chip - Google Patents

Plastic chip Download PDF

Info

Publication number
CN2775659Y
CN2775659Y CN 200520039760 CN200520039760U CN2775659Y CN 2775659 Y CN2775659 Y CN 2775659Y CN 200520039760 CN200520039760 CN 200520039760 CN 200520039760 U CN200520039760 U CN 200520039760U CN 2775659 Y CN2775659 Y CN 2775659Y
Authority
CN
Grant status
Grant
Patent type
Prior art keywords
chip
jig
lower
plastic
reaction plate
Prior art date
Application number
CN 200520039760
Other languages
Chinese (zh)
Inventor
穆海东
Original Assignee
穆海东
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Grant date

Links

Abstract

The utility model relates to a plastic chip, which relates to the fields of biological chip technology and clinical diagnosis detection technology in vitro, and can be applied to clinical disease detection of the parallelization of multiple indexes and multiple persons. The utility model is composed of an upper fixture, a lower fixture, a chip strip and a reaction plate, wherein the chip strip adopts plastics which have affinity to bioactive samples as materials, and can be detached and free combined. After the chip strip is fixed by the upper and the lower fixtures, a biological sample dot matrix is formed on the chip strip with a high-speed spotting apparatus, and then the utility model is buckled on the reaction plate filled with serum or samples and reaction liquid to be detected to carry out reaction. Compared with the prior art, the utility model has the advantages of low background signal value, convenience for manufacture and use and low cost.

Description

说明书一种塑料芯片 A plastic chip specification

技术领域 FIELD

本实用新型涉及生物芯片技术和体外临床诊断检测技术领域,更确切的说,涉及一种适合于多指标、多人份并行检测的生物芯片。 The present invention relates to a bio-chip technology and in vitro clinical diagnostic tests, and more specifically, relates to a method suitable for multi-index, than parts biochip sensed in parallel.

背景技术 Background technique

生物芯片的概念源于半导体、计算机芯片,主要是指包被在固相载体上的DNA微阵列、寡核苷酸微阵列和蛋白质微阵列。 Concept biochip from a semiconductor, computer chips, mainly refers to a DNA microarray coated on a solid phase support, oligonucleotide microarrays and protein microarrays. 目前常见的生物芯片分为三大类:即基因芯片、蛋白芯片、芯片实验室。 Current common biochip divided into three categories:,, namely laboratory chip protein chip gene chip. 生物芯片主要特点是高通量、微型化和自动化,能够在很短时间内分析大量的生物分子,快速准确地获取样品中的生物信息,检测效率是传统检测手段的成百上千倍。 Biochip main feature is a high-throughput, miniaturization and automation, in a very short period of time can analyze large amounts of biological molecules, biological information quickly and accurately obtain the sample, the detection efficiency is hundreds of times faster than traditional detection methods.

目前国内外生物芯片常用的固相载体有玻片和尼龙膜两大类。 At home and abroad biochip conventional solid carriers are two major categories of slides and a nylon film. 玻片主要用于基因芯片,具有廉价、检测本底低的优点,但是在点样前需要对玻片表面进行复杂的化学处理,难以加工、易碎。 Mainly for microarray slides, having a low cost, the advantage of low background detection, but requires complicated chemical treatment before a glass slide spotted difficult to work, brittle. 尼龙膜虽然对生物分子吸附性好,有利于保持生物分子天然活性,但是通常背景信号值高,目前主要依赖进口,价格昂贵。 While the nylon membrane adsorption of biomolecules is good, it is conducive to maintaining the natural activity of biological molecules, but are usually high background signal value, mainly rely on imports, the price is expensive.

因此本领域迫切需要开发一种以背景信号值低、廉价、易使用的材料作为固相载体的生物芯片。 Thus there is an urgent need to develop a low background signal values, inexpensive, easy to use the material as a solid support of the biochip. 塑料是一种理想的制备生物芯片的材料。 Plastic is a desirable material for biochip preparation. 塑料对蛋白的吸附能力适中,显著克服了尼龙膜背景信号值高的缺点。 Moderate protein adsorption capacity of plastic significantly overcome the high background signal values ​​nylon membrane disadvantages. 塑料还容易加工成各种形状、不易碎,价格低廉,并且在点样前只需简单处理就能使用。 Plastic is also easily processed into a variety of shapes, unbreakable, inexpensive, and can be used simply treated before spotting. 因此我们以塑料为材料开发了一种塑料芯片,除具备上述制作、使用方便等优点外,芯片条还可以自由拆卸、组合,使得临床检测的指标和人份数都可以自由组合,更适应临床检测的实际需要。 Therefore, we developed a plastic material is a plastic chip, in addition to having the above production, ease of use, the article can freely detachable chip, a combination of such indicators, and human clinical testing parts may be freely combined, more suit the clinical actually be detected.

发明内容 SUMMARY

本实用新型的目的在于针对上述问题,提供一种可应用于临床的多指标、多人份并行化疾病检测生物芯片。 The present invention aims to solve these problems, there is provided a multi-index which can be applied clinically, than parts parallelization disease detection biochip. 提供一种低背景、低成本、操作简便的芯片系统。 To provide a low background, low cost, easy-to-chip system.

本实用新型提供了一种塑料生物芯片,其特征在于,它含有以下组件:——芯片条(1),所述芯片条具有凸起的水平端面以及便于上、下夹具夹紧固定的底座;—上夹具(2),所述上夹具为一四边有框底部密封盒,同时四边具有便于与下夹具咬合的突起或孔槽;—下夹具(3),所述下夹具具有与芯片凸起水平端面相对应的孔,同时四边具有便于与上夹具咬合的突起或孔槽;—反应板(4),所述反应板具有与芯片条凸起端面相对应的密封孔,孔内可以添加反应液便于芯片条端面接触进行生化反应;其中,各个组件是相互分离的,且装配在一起时,所述的芯片条(1)、上下夹具(2、3)及反应板(4)的各部位相互对应,结合紧密。 The present invention provides a plastic biochip, characterized in that it comprises the following components: - Article chip (1), said chip having a horizontal bar and the end surface of the projection to facilitate upper and lower clamping fixture fixed base; - a jig (2), the four sides of the jig is a sealed box with a bottom frame, while having four sides to facilitate engagement with the lower jig protrusion or slot holes; - the clamp (3), said chip having a convex lower clamp since the horizontal end surface of the corresponding hole, and having four sides to facilitate engagement with the projection on the clamp grooves or holes; - the reaction plate (4), the reaction plate having a hole and a sealing end face of the chip corresponding to the protrusion section, may be added to the hole the reaction liquid facilitates contacting the chip strip ends biochemical reaction; wherein various components are separated from each other, and are assembled together, the chip strip (1), upper and lower jaws (2, 3) and reaction plate (4) each parts correspond to each other tightly bound.

在一优选例中,芯片条(1)、上夹具(2)、下夹具(3)、反应板(4)的形状、大小、孔阵列的位置都是一一对应,能完全吻合的。 In a preferred embodiment, the chip strip (1), the clamp (2), the lower clamp (3), the reaction plate (4) the position of the shape, size, array of apertures is one to one, completely consistent.

在另一优选例中,芯片条(1)的材料选用聚苯乙烯材料或其他具有对生物活性样本(DNA、蛋白质等)吸附性的材料。 In another preferred embodiment, the chip (1) The selection of materials of polystyrene or other materials having biological activity adsorbent samples (DNA, proteins, etc.).

在另一优选例中,芯片条(1)可以方便从上下夹具(2、3)中拆卸,自由组合所需要的检测人份。 In another preferred embodiment, the die bar (1) can be easily detached from the upper and lower jaws (2, 3), consisting of a combination of parts needed to detect human.

在另一优选例中,下夹具(3)与反应板(4)所对应的孔的数目为48、96、128、384。 In another preferred embodiment, the number of the lower clamp (3) and reaction plate (4) corresponding to the holes is 48,96,128,384.

在另一优选例中,所述的芯片条(1)形状、大小与下夹具(3)孔的大小形状一致,外型基本为矩形、六边形、八边形或圆形。 In another preferred embodiment, the chip (1) The shape, size, and lower jig (3) uniform size and shape of the aperture, a substantially rectangular shape, hexagonal, octagonal or circular.

在另一优选例中,所述的芯片条(1)与上下夹具(2、3)装配体完成后,可以倒扣在反应板(4)里进行免疫、杂交、生化等各种反应。 In another preferred embodiment, the chip strip (1) and the upper and lower jaws (2,3) after the assembly is completed, the reaction may be upside down plate (4) in a variety of immunological reactions, hybridization, and biochemistry.

在另一优选例中,所述的上、下夹具(2、3)与反应板(4)的材料选自下组:塑料、橡胶或金属及所有可以使用的材料。 In another preferred embodiment, the upper and lower clamps (2,3) and reaction plate (4) a material selected from the group: plastic, rubber or metallic materials, and all can be used.

附图说明 BRIEF DESCRIPTION

附图是本实用新型的一种实例的部件分解图。 BRIEF member is a new example of an exploded view of the present invention.

具体实施方案如图所示,本实用新型的生物芯片包括上夹具2、下夹具3、芯片条1、反应板4四个组件,各组件相互独立,此外,还含有任选的固定方式(凸起或孔槽等),用于将上述组件固定组合在一起。 Fixing the specific embodiments shown in FIG embodiment, the present invention includes a biochip jig 2, the jig 3, the chip 1 of the reaction plate 4 four components, each independently of each other, in addition, optionally further comprising (convex since holes or slots, etc.), for the above-described components secured together.

下而结合具体实施例,进一步阐述本实用新型。 Incorporated at specific embodiments, further illustrate the present invention.

实施例(1)将芯片条1放入下夹具对应孔内,将上、下夹具2、3合紧,固定芯片条1。 Example (1) 1 of the chip into the jig hole corresponding to the upper and lower clamps 2,3 engagement for fixing a chip strip.

(2)用高速点样仪在芯片条1上端面构建生物样品(DNA、蛋白质等)点阵。 (2) Construction of a high speed end face spotter biological samples (DNA, proteins, etc.) on the chip 1 of the lattice.

(3)在反应板4孔中依次加入待检测血清样品及相关反应液,将合紧的上下夹具及芯片条装配体倒扣入反应板进行孵育、反应。 (3) a serum sample to be tested were added and the reaction solution related well reaction plate 4, the upper and lower clamp is securely closed and the assembly flip-chip article into the reaction plate was incubated reaction.

(4)按常规洗涤方式进行冲洗。 (4) rinsing a conventional washing method.

(5)将洗涤完的上、下夹具2、3和芯片条1装配体放入检测仪进行检测。 (5) After the upper washing, the lower jig 2, 3 and detecting chip strips in a detector assembly.

应理解,在阅读了本实用新型的上述讲授内容之后,本领域技术人员可以对本实用新型作各种改动或修改,这些等价形式同样落于本申请所附权利要求书所限定的范围。 It should be understood, after reading the disclosure of the present teachings, those skilled in the art of the present invention can make various changes or modifications of these equivalents also fall within the scope of the appended claims of the present application as defined.

Claims (6)

  1. 1.一种塑料芯片,其特征在于,它含有以下组件:—芯片条(1),所述芯片条具有凸起的水平端面以及便于上、下夹具夹紧固定的底座;—上夹具(2),所述上夹具为一四边有框底部密封盒,同时四边具有便于与下夹具咬合的突起或孔槽;—下夹具(3),所述下夹具具有与芯片凸起水平端面相对应的孔,同时四边具有便于与上夹具咬合的突起或孔槽;—反应板(4),所述反应板具有与芯片条凸起端面相对应的密封孔,孔内可以添加反应液便于芯片条端面接触进行生化反应;其中,各个组件是相互分离的,且装配在一起时,所述的芯片条(1)、上下夹具(2、3)及反应板(4)的各部位相互对应,结合紧密。 1. A plastic chip, characterized in that it comprises the following components: - Article chip (1), said chip having an upper strip end surface and a horizontal projection convenience, the jig clamping the fixed base; - a jig (2 ), the four sides of the jig is a sealed box with a bottom frame, while having four sides to facilitate engagement with the lower jig protrusion or slot holes; - the clamp (3), the lower jig having a horizontal projection of the chip corresponding to the end face holes, while four sides of the jig to facilitate engagement with the projection or slot holes; - the reaction plate (4), the reaction plate having a hole and a sealing end face of the chip corresponding to the projection section, the hole may be added to facilitate the reaction solution chip article contacting the end face biochemical reaction; wherein the individual components are separated from each other, and when assembled, the chip strip (1), upper and lower jaws (2, 3) and reaction plate (4) of the various parts correspond to each other, with close.
  2. 2.根据权利要求1所述的塑料芯片,其特征在于,芯片条(1)、上夹具(2)、下夹具(3)、反应板(4)的形状、大小、孔阵列的位置都是一一对应,能完全吻合的。 The plastic chip according to claim 1, characterized in that the chip strip (1), the clamp (2), the lower clamp (3), the shape of the position of reaction plate (4), the size of the aperture array are correspondence, completely consistent.
  3. 3.根据权利要求1所述的塑料芯片,其特征在于,芯片条(1)的材料选用聚苯乙烯材料或其他具有对生物活性样本吸附性的材料,生物活性样本可以是DNA、蛋白质。 3. The plastic chip according to claim 1, characterized in that the chip (1) The selection of materials of polystyrene or other materials having adsorptivity on the biological activity of the sample, the sample may be a biological activity of DNA, proteins.
  4. 4.根据权利要求1所述的塑料芯片,其特征在于,下夹具(3)与反应板(4)所对应的孔的数目为48、96、128、384。 Plastic chip according to claim 1, wherein the number of the lower clamp (3) and reaction plate (4) corresponding to the holes is 48,96,128,384.
  5. 5.根据权利要求1所述的塑料芯片,其特征在于,所述的芯片条(1)形状、大小与下夹具(3)孔的大小形状一致,外型基本为矩形、六边形、八边形或圆形。 The plastic chip according to claim 1, characterized in that, consistent with the size and shape of the chip (1) The shape, size, and lower jig (3) hole shape substantially rectangular, hexagonal, eight polygonal or circular.
  6. 6.根据权利要求1所述的塑料芯片,其特征在于,所述的上、下夹具(2、3)与反应板(4)的材料选自下组:塑料、橡胶或金属。 The plastic chip according to claim 1, characterized in that, on the material of the lower clamp (2, 3) and reaction plate (4) is selected from the group consisting of: plastic, rubber or metal.
CN 200520039760 2005-02-24 2005-02-24 Plastic chip CN2775659Y (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 200520039760 CN2775659Y (en) 2005-02-24 2005-02-24 Plastic chip

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 200520039760 CN2775659Y (en) 2005-02-24 2005-02-24 Plastic chip

Publications (1)

Publication Number Publication Date
CN2775659Y true CN2775659Y (en) 2006-04-26

Family

ID=36749974

Family Applications (1)

Application Number Title Priority Date Filing Date
CN 200520039760 CN2775659Y (en) 2005-02-24 2005-02-24 Plastic chip

Country Status (1)

Country Link
CN (1) CN2775659Y (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104010577A (en) * 2011-11-30 2014-08-27 通用电气医疗集团英国有限公司 Biological sample storage apparatus and method
CN107389933A (en) * 2017-06-14 2017-11-24 杨华卫 Biological chip

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104010577A (en) * 2011-11-30 2014-08-27 通用电气医疗集团英国有限公司 Biological sample storage apparatus and method
CN107389933A (en) * 2017-06-14 2017-11-24 杨华卫 Biological chip

Similar Documents

Publication Publication Date Title
Venkatasubbarao Microarrays–status and prospects
Mezzasoma et al. Antigen microarrays for serodiagnosis of infectious diseases
Ruzicka et al. Peer Reviewed: From Flow Injection to Bead Injection.
US6677131B2 (en) Well frame including connectors for biological fluids
US7682565B2 (en) Assay apparatus and method using microfluidic arrays
US6054100A (en) Apparatus for multi-well microscale synthesis
US20020119470A1 (en) Magnetic bead-based array for genetic detection
US20030044799A1 (en) Analysis using a distributed sample
US20050164264A1 (en) Method and system for rapid biomolecular recognition of amino acids and protein sequencing
Hortin et al. Proteomics: a new diagnostic frontier
US20040141887A1 (en) Apparatus and methods to process substrate surface features
Michael et al. Randomly ordered addressable high-density optical sensor arrays
US6861214B1 (en) Immobilization of biopolymers to aminated substrates by direct adsorption
US20070292837A1 (en) Multiwell Plate
US20040071605A1 (en) Slide-based high-throughput microplate device
US5244788A (en) Method and apparatus for performing determinations of immune rectants in biological fluids
US4822565A (en) Carrier device for immunological assay
US20010036630A1 (en) Purification method and apparatus
WO1999064867A1 (en) Multiple assay method
Templin et al. Protein microarrays and multiplexed sandwich immunoassays: what beats the beads?
US20030040129A1 (en) Binding assays using magnetically immobilized arrays
US20050135974A1 (en) Device for preparing multiple assay samples using multiple array surfaces
US20050254995A1 (en) Devices and methods to immobilize analytes of interest
Kumble Protein microarrays: new tools for pharmaceutical development
WO2001014425A1 (en) Multipurpose diagnostic systems using protein chips

Legal Events

Date Code Title Description
C14 Granted
LIC Patent license contract for exploitation submitted for record

Effective date: 20081021

Free format text: EXCLUSIVE LICENSE; TIME LIMIT OF IMPLEMENTING CONTACT: 2008.8.8 TO 2013.8.8; CHANGE OF CONTRACT

Name of requester: SHANGHAI YULONG BIOLOGICAL TECHNOLOGY CO., LTD.

C17 Cessation of patent right
LICC Enforcement, change and cancellation of record of contracts on the license for exploitation of a patent