CN206192994U - Test device is avoided to soil springtail - Google Patents
Test device is avoided to soil springtail Download PDFInfo
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- CN206192994U CN206192994U CN201621298308.XU CN201621298308U CN206192994U CN 206192994 U CN206192994 U CN 206192994U CN 201621298308 U CN201621298308 U CN 201621298308U CN 206192994 U CN206192994 U CN 206192994U
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- soil
- culture apparatus
- springtail
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Abstract
The utility model discloses a test device is avoided to soil springtail, including culture apparatus's lid, distinguish the baffle of handling soil and contrast soil, culture apparatus and collection device, the culture apparatus bottom is equipped with foraminiferous sieve and not foraminiferous bottom plate. Be equipped with axisymmetric rail groove on culture apparatus and the collection device internal surface respectively, the baffle passes through the rail groove and pegs graft with culture apparatus and collection device. The problem of present springtail avoidance test water float glass process complex operation, insert back that the position of baffle uncertainly causes that error, the easy loss of springtail cause that data are invalid again, the springtail causes error etc. From baffle one side to in addition on one side of along with the gap is solved.
Description
Technical field
The utility model belongs to soil animal avoidance test engineering device technique field, and in particular to a kind of soil-natural TL avoids examination
Experiment device.
Background technology
With the deterioration of earth environment, people are to environmental problem pay attention to day by day, and product of the poisonous and harmful substance in soil
Tire out the stability and sustainability that can influence soil ecosystem.Due to the limitation of Soil Contamination Evaluation methods and techniques, for a long time
Since the aspects such as concentration and chemical form of the detection polluter in soil are concentrated on to the research of soil quality assessment, for
Interaction between pollutant, soil and biological three then rarely has consideration, and reason is to be difficult to assessment pollutant to soil ring
The bio-toxicity effect in border.Over nearly 20 years, ecological risk assessment gradually rises, and is with ecology, toxicology, biology and chemistry
Theoretical foundation, the sci tech evaluation such as integrated use mathematics and computer triggers unfavorable under one or more stress factors
The process of ecological effect possibility.Terrestrial invertebrate is widely distributed in soil, is the important composition of soil ecosystem
Part.Need that the sensitiveness and tolerance and assessment endpoint of pollutant are selected to close according to species in actual risk assessment
Suitable sensitive species.Springtail is more sensitive to soil pollution, tests to evaluate soil pollution life by the eco-toxicity to springtail
The certain Research foundation of state risk, but the indexs such as the death rate, breeding potential are had focused largely on, belong to chronic toxicity test.Cause
This, with simple and quick, sensitivity is high and avoidance the features such as favorable reproducibility is tested in terms of contaminated soil ecological risk assessment
It is widely used.It is long less than 1mm that the white symbol of the small volume of springtail, such as 9-12d jumps larva body.Traditional springtail avoidance test method
It is that the operation of ISO directive/guides combines water float glass process, comprises the concrete steps that and utilize cylindrical container, insertion plastic septum is divided into the two of equalization
Part a, part adds treatment soil 30g, another part to add control soil 30g, then take out dividing plate, added in midline
The white symbol of 9-12d jumps 20, and in 20 ± 1 DEG C of temperature, illumination 400-800Lux, round the clock than being 16h:Culture 48 is small under the conditions of 8h
When, dividing plate is turned back to again, a certain amount of water then is separately added on both sides, springtail is suspended on the water surface (water float glass process), respectively
Two-part springtail is counted, data invalid when loss springtail number is more than 20%.Because the volume of springtail is minimum, use
Traditional apparatus and method are tested, when dividing plate is turned back to again, it is impossible to which guarantee turns back to dividing plate with same position, may lead
Control soil is caused to be assigned in treatment soil, the springtail in control soil is also assigned in treatment soil therewith, causes error；Jump
Worm easily loses, and causes experimental data inaccurate or invalid；Secondly, springtail easily passes through plastic septum from treatment soil part
The gap at edge enters into control treatment, increases test error；In addition, being operated using water float glass process comparatively laborious.So, design one
It is springtail avoidance test problem demanding prompt solution that set can overcome the device of disadvantages mentioned above and set up relevant art method.
Directly using water float glass process the drawbacks of：Different types of soil occurs white or brown bubble after the stirring that adds water
There are some fine suspension matter (organic matter) in foam, and soil, and because of springtail small volume, white in itself, it is easy to
Hidden in foam, so that it cannot count or count inaccurate.
Utility model content
The technical problem of solution：The utility model proposes a kind of soil-natural TL avoidance test device, current springtail is solved
Avoidance test water float glass process is cumbersome, turn back to the position of dividing plate again does not know to cause the easy loss of error, springtail to cause data
The problems such as invalid, springtail causes error from dividing plate side with gap to other side.
Technical scheme：A kind of soil-natural TL avoidance test device, including dividing plate and set gradually from top to bottom culture dress
Put lid, culture apparatus and collection device；
The culture apparatus includes sieve plate, base plate and pin-and-hole, and sieve plate level is located at the bottom of culture apparatus, the sieve plate edge
Radially it is divided into two；Base plate is radially divided into two located at the lower section of sieve plate, and the pin-and-hole is outside the bottom of culture apparatus
Side；
The top outside of the collection device is provided with alignment pin, and the alignment pin is connected with pin-and-hole dismounting；
Axisymmetric track slot is respectively equipped with the culture apparatus and collection device inner surface, dividing plate is by track slot and culture
Device and collection device grafting.
Preferably, the pin-and-hole is respectively arranged on the outside of culture apparatus bottom track slot.
Preferably, the sieve plate is two semicircle PVC plastic flitch, contains on described each semicircle and be uniformly distributed interval
It is 3 × 3mm, the aperture of a diameter of 2mm, when two semicircle PVC plastic flitch of the sieve plate close up rounded, in its junction
Upper surface form groove, and the two ends of groove connect with track slot respectively；The edge of the semicircle PVC plastic flitch is provided with circular arc
The handle of shape.
Preferably, the base plate is two semicircle PVC plastic flitch, two semicircle PVC plastic flitch of the base plate
When closing up rounded, groove is formed in the upper surface of its junction, and the two ends of groove connect with track slot respectively；The semicircle
The edge of PVC plastic flitch is provided with the handle of arc-shaped.
Preferably, the alignment pin is respectively arranged on the outside of collection device track slot, and it is protruding upward corresponding with pin-and-hole.
Preferably, the dividing plate is the PVC plastic flitch that bottom is fined away.
Beneficial effect：The utility model is quickly connected using the alignment pin of dividing plate, the pin-and-hole of culture apparatus and collection device
Culture apparatus and collection device, can avoid grogs from being scattered, and cause error.
The utility model is inserted dividing plate using track slot, exactly will treatment soil and control soil point when turning back to again
Open, it is to avoid when turning back to dividing plate will treatment soil assign to control soil in or will control soil assign to treatment soil in.
The utility model utilizes sieve plate and base plate, on the one hand will not fall out soil when culture, the opposing party
Soil is driven to be leaked under space when face can prevent dividing plate from twitching；Culture extracts base plate out after finishing, and the baking of worm equipment is dried utilizing
Can make springtail that collection device is entered into from the hole of sieve plate when worm.
When two semicircle PVC plastic flitch of sieve plate described in the utility model and base plate close up rounded, in its junction
Upper surface formed groove, culture complete turn back to dividing plate again when can be separated by partition, make dividing plate along track slot insert receive
Acquisition means bottom.
The utility model is provided with dividing plate, and treatment soil and control soil are separated into two parts, makes experiment more accurate.
The utility model carries out baking worm using worm equipment is dried, and increases the probability of data validity.
The utility model uses sieve plate and base plate, can disassembly and assembly rapidly, it is convenient test after clean culture device.
Brief description of the drawings
Fig. 1 is the structural representation of soil-natural TL avoidance test device；
Fig. 2 is the structural representation of culture apparatus in soil-natural TL avoidance test device；
Fig. 3 is the top view of culture apparatus in soil-natural TL avoidance test device；
Fig. 4 is the bottom schematic view of culture apparatus in soil-natural TL avoidance test device；
Fig. 5 is the side view of culture apparatus in soil-natural TL avoidance test device；
Fig. 6 is the structural representation of sieve plate in soil-natural TL avoidance test device；
Fig. 7 is the structural representation of base plate in soil-natural TL avoidance test device；
Fig. 8 is the structural representation of collection device in soil-natural TL avoidance test device；
Fig. 9 is the structural representation of soil-natural TL avoidance test device median septum.
In figure, each digitized representation implication is as follows：1. culture apparatus lid；2. dividing plate；3. track slot；4. culture apparatus；5a. is sieved
Plate；5b. base plates；6. collection device；7a. pin-and-holes A；7b. pin-and-holes B；8a. handles A；8b. handles B；9a. annular arc grooves A；
9b. annular arc grooves B；10a. alignment pins A；10b. alignment pins B；11a. handles C；11b. handles D.
Specific embodiment
The utility model is described in detail with reference to the accompanying drawings and detailed description.
Embodiment 1
As shown in figure 1, including culture apparatus lid 1, the dividing plate 2 of differentiation treatment soil and control soil and by pin-and-hole
The annular culture apparatus 4 and collection device 6 quickly connected with alignment pin.
It is respectively equipped with axisymmetric track slot 3 on culture apparatus 4 and the inner surface of collection device 6, the depth of the track slot 3 2mm is wide
2mm。
Culture apparatus 4 includes sieve plate 5a, base plate 5b and pin-and-hole, and the pin-and-hole includes pin-and-hole A7a and pin-and-hole B7b.The training
It is the transparent hollow cylinder of internal diameter 55mm, thick 5mm, 55mm high to support device 4, and the bottom of the culture apparatus 4 is located at track slot
Outside is respectively symmetrically and is provided with pin-and-hole A 7a and pin-and-hole B 7b, the pin-and-hole A 7a and pin-and-hole B 7b depth 2mm, width 3mm, height
1mm。
As shown in figure 5, the bottom of the culture apparatus 4, two diameter 59mm are respectively equipped with track slot vertical direction, height
The annular arc groove A 9a and annular arc groove B 9b of 1mm, two grooves, at a distance of 5mm, are 1mm apart from bottom level, and specification is
1.5mm wide, sieve plate 5a and base plate 5b are corresponded in inserting in the annular arc groove A 9a and annular arc groove B 9b respectively.
The extraction plate 5a of the culture apparatus 4 is PVC plastic flitch, and two semicircles of thick 1mm, a diameter of 58mm are described every
Containing the aperture for being uniformly distributed diameter 2mm on individual semicircle, at intervals of 3mm × 3mm, and diameter 69mm is added in semicircular edge, it is thick
The circular arc of 1mm is spent as handle, the handle there are two, is respectively handle A8a and handle B 8b, can conveniently pull out semicircle
Base plate.When the semicircle PVC plastic flitch of two of the sieve plate closes up rounded, groove is formed in the upper surface of its junction, and
The two ends of groove connect with track slot respectively.
The bottom base panel 5b of the culture apparatus 4 is PVC plastic flitch, and two semicircles of thick 1mm, a diameter of 58mm are described every
Individual semicircular edge adds diameter 69mm, and used as handle, the handle has two to the circular arc of thickness 1mm, be respectively handle C 11a and
Handle D 11b, can conveniently pull out semicircle base plate.When the semicircle PVC plastic flitch of two of the base plate closes up rounded,
The upper surface of its junction forms groove, and the two ends of groove connect with track slot respectively.
The treatment soil and the dividing plate 2 of blank soil distinguished is PVC plastic flitch, thickness 1mm, 110mm long, width
58mm.The bottom of the dividing plate 2 is fined away, in convenient insertion soil.
The collection device 6 is internal diameter 55mm, thick 5mm, the upper opening of 55mm high and hollow transparent cylinder.
Along track slot outside on the collection device, there is alignment pin protruding upward at two ends respectively, and the alignment pin includes alignment pin A 10a
Diameter 2mm is with alignment pin B 10b, the alignment pin A 10a and alignment pin B 10b, the cylinder of 1mm high can be corresponded to
In pin-and-hole A 7a and pin-and-hole the B 7b of insertion culture apparatus 4.
Above-mentioned treatment soil and control soil were the soil of 2mm sieves, and adjusted soil moisture content according to ISO directive/guides.
The specific steps of the utility model operation：When beginning is cultivated, by sieve plate 5a and base plate 5b not with holes with holes
The bottom of culture apparatus 4 is inserted, the dividing plate 2 that will distinguish treatment soil and control soil inserts culture apparatus 4 along track slot 3, then
Wet soil (the 50% of field capacity) each 30g for the treatment of soil and control soil is weighed in two locellus respectively, dividing plate 2 is extracted out,
Closure base plate 5b, culture apparatus lid 1 is covered, be put into constant incubator 20 ± 1 DEG C, illumination 400-800Lux, compare round the clock
16h:8h is cultivated 48 hours, and culture apparatus lid 1 is taken down, and 20mL deionized waters is poured into collection device 6, and fill collecting
Put 6 to be connected by the way that pin-and-hole A 7a and pin-and-hole B 7b and alignment pin A 10a and alignment pin B 10b are corresponding with culture apparatus 4, by dividing plate
2 turn back to again, after dividing plate 2 is inserted into the bottom of collection device 6 along track slot 3, extract base plate 5b not with holes out and observe base plate
5b both sides springtail number, whole device is put into baking worm equipment and dries worm, the springtail in culture apparatus 4 is baked out collection device 6
In, culture apparatus 4 is then taken down, and dividing plate 2 is stayed in collection device 6, several melted inks are added in two locellus in left and right, so
The springtail number in treatment soil and control soil is counted respectively using water float glass process afterwards, is added and processed on base plate 5b soil and control soil
Springtail number in earth, according to the formula of rate of avoidance, calculates rate of avoidance of the springtail to the contaminated soil.
The cleaning of device：Experiment finish after, remove the sieve plate 5a of the bottom of which has holes of culture apparatus 4, will distinguish treatment soil and
Dividing plate 2, culture apparatus 4, collection device 6 and the base plate 5b for compareing soil are cleaned, and are air-dried in the cool so as to next profit
With.
Four kinds of different types of soil are chosen, contrast avoidance test directive/guide operation combines water float glass process, directive/guide operation and combines and dry
The operation of worm unit combines water float glass process and device operation combines the extraction efficiency and rate of avoidance for drying worm four kinds of methods of equipment
(directive/guide is ISO directive/guides).
The utility model of table 1 is contrasted with existing conventional apparatus and method extraction efficiency
Directive/guide combination water float glass process extracts springtail number between 8-16 heads, device combination water float glass process extraction springtail number between
11-16 heads, the two is more or less the same, because when water float glass process extracts springtail, gently being disturbed in the soil body with blade, will may jump
Worm kills, or foam on the water surface and floods springtail, it is impossible to clear to count.
It is 16-20 heads that directive/guide combines baking worm equipment and extracts springtail number, and device combination is dried worm equipment extraction springtail number and is
19-20 heads, extract number and are generally higher than directive/guide and combine and dry worm equipment, because directive/guide is combined dries worm equipment, after having cultivated, need
The soil of the right and left is transferred out, springtail may be caused to lose during transfer.
The extraction springtail number that device combines baking worm equipment is most, and utilizes grooved tracks, can will quickly compare soil
Earth and treatment soil are separated, it is to avoid cause springtail to lose using water float glass process, and also avoiding can not be complete by control soil and treatment soil
Separately produce error entirely.Device combines baking worm equipment and can improve experimental accuracy, the error produced during reduction.
The utility model is starting point to improve the degree of accuracy of springtail avoidance test and the difficulty of reduction operation, there is provided one
Plant soil-natural TL avoidance test device.Main design thought：1st, claim soil in left and right locellus, extract dividing plate out, after the completion of culture,
When turning back to dividing plate again, ensure that dividing plate will process soil and control soil is completely separable using track slot, it is to avoid treatment soil
Earth and control soil can not be distinguished completely, cause experimental error.2nd, drawable type base plate and sieve plate are utilized respectively, are kept away in culture
Exempt from soil to leak down from sieve plate, drive soil to be leaked under space when preventing dividing plate from twitching, while will not be with holes after culture is finished
Base plate extract out, using dry worm equipment dry worm when allow springtail from the hole of sieve plate enter collection device.3rd, using baking
Worm equipment separates soil and springtail, then carries out take pictures counting or the floating directly counting of water, can avoid directly entering water-filling float glass process
Foam is produced in container, springtail quantity is reduced, experimental error is caused.4th, can be reduced using the utility model apparatus and method
The troublesome operation of conventional method, makes experiment simple, and improves experiment accuracy rate.
Claims (6)
1. a kind of soil-natural TL avoidance test device, it is characterised in that：The soil-natural TL avoidance test device includes dividing plate (2)
Culture apparatus lid (1), culture apparatus (4) and the collection device (6) for setting gradually from top to bottom；
The culture apparatus (4) includes sieve plate (5a), base plate (5b) and pin-and-hole, and sieve plate (5a) level is located at culture apparatus (4)
Bottom, the sieve plate (5a) is radially divided into two；Base plate (5b) is radially divided into two located at the lower section of sieve plate (5a),
Basolateral of the pin-and-hole located at culture apparatus (4)；
The top outside of the collection device (6) is provided with alignment pin, and the alignment pin is connected with pin-and-hole dismounting；
Axisymmetric track slot (3) is respectively equipped with the culture apparatus (4) and collection device (6) inner surface, dividing plate (2) is by rail
Groove (3) and culture apparatus (4) and collection device (6) grafting.
2. soil-natural TL avoidance test device according to claim 1, it is characterised in that：The pin-and-hole is respectively arranged on culture
The outside of device (4) bottom track slot.
3. soil-natural TL avoidance test device according to claim 1, it is characterised in that：The sieve plate (5a) is two and half
Circular PVC plastic flitch, contains on described each semicircle and is uniformly distributed at intervals of 3 × 3mm, the aperture of a diameter of 2mm, the sieve plate
When two of (5a) semicircle PVC plastic flitch close up rounded, groove is formed in the upper surface of its junction, and groove two ends
Connect with track slot respectively；The edge of the semicircle PVC plastic flitch is provided with the handle of arc-shaped.
4. soil-natural TL avoidance test device according to claim 1, it is characterised in that：The base plate (5b) is two and half
Circular PVC plastic flitch, when two semicircle PVC plastic flitch of the base plate (5b) close up rounded, in the upper table of its junction
Face forms groove, and the two ends of groove connect with track slot respectively；The edge of the semicircle PVC plastic flitch is provided with the handle of arc-shaped
Hand.
5. soil-natural TL avoidance test device according to claim 1, it is characterised in that：The alignment pin is respectively arranged on receipts
The outside of acquisition means (6) top track slot, and it is protruding upward corresponding with pin-and-hole.
6. soil-natural TL avoidance test device according to claim 1, it is characterised in that：The dividing plate (2) is to cut bottom
The PVC plastic flitch of point.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621298308.XU CN206192994U (en) | 2016-11-29 | 2016-11-29 | Test device is avoided to soil springtail |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621298308.XU CN206192994U (en) | 2016-11-29 | 2016-11-29 | Test device is avoided to soil springtail |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206192994U true CN206192994U (en) | 2017-05-24 |
Family
ID=58725504
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621298308.XU Expired - Fee Related CN206192994U (en) | 2016-11-29 | 2016-11-29 | Test device is avoided to soil springtail |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206192994U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106706880A (en) * | 2016-11-29 | 2017-05-24 | 中国科学院南京土壤研究所 | Springtail avoidance test device and application thereof |
| CN114594236A (en) * | 2022-03-15 | 2022-06-07 | 西北农林科技大学 | Simple and rapid investigation method for soil springtail of edible fungi |
-
2016
- 2016-11-29 CN CN201621298308.XU patent/CN206192994U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106706880A (en) * | 2016-11-29 | 2017-05-24 | 中国科学院南京土壤研究所 | Springtail avoidance test device and application thereof |
| CN114594236A (en) * | 2022-03-15 | 2022-06-07 | 西北农林科技大学 | Simple and rapid investigation method for soil springtail of edible fungi |
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| Date | Code | Title | Description |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170524 Termination date: 20171129 |