CN2407012Y

CN2407012Y - Disposable box for testing escherichia coli

Info

Publication number: CN2407012Y
Application number: CN 99255591
Authority: CN
Inventors: 高晓春; 郭少林
Original assignee: Individual
Current assignee: Individual
Priority date: 1999-12-08
Filing date: 1999-12-08
Publication date: 2000-11-22
Anticipated expiration: 2009-12-08

Abstract

The utility model relates to a rectangular box body with a cover. A partition board is arranged in the box body, one side of the partition board is provided with a reaction plate frame and a small transparent bottle, and the other side of the partition board is placed with a small bottle and a dropper respectively held with thyme phenolphthalein reagent and eosine methylene agar, wherein, a plate hole is arranged in the reaction plate frame, and the small transparent bottle is arranged in the plate hole; the small transparent bottle is composed of a bottle body, a rubber ring is sheathed out of the bottle body, and the inner part of the bottle body is provided with a culture medium. A test paper bracket is arranged in a bottle cap, test paper is placed on the test paper bracket, and the bottle cap is covered on a bottle mouth of the small transparent bottle.

Description

Disposable coliform detection kit

The utility model is a kind of disposable coliform detection kit, belongs to the Micro biological Tests articles for use.

At present, China's detection coliform ordinary method is the test tube fermentation method.This kind method complex operation, detection time is long, the substratum consumption is big, is difficult for prolonged preservation, used apparatus is many, with after need scrub in a large number, elapsed time is long, waste of manpower and material resources.

The purpose of this utility model is: a kind of disposable coliform detection kit is provided, this detection kit is simple to operate, easy to use, assay is accurate, quick, with low cost, with after need not scrub, easy to carry, be easy to prolonged preservation, can save human and material resources and financial resources.

A kind of disposable coliform detection kit of the utility model, it has rectangle box body with cover, in box body, be provided with dividing plate 15, side at dividing plate 15 is equipped with reaction grillage 3 and transparent bottle 4, and opposite side is being put the bottle that thymolphthalein reagent 11 is housed, the bottle and the dropper 13 of eosin methylene blue agar 12 respectively; Wherein:

This reaction grillage 3 is provided with plate hole 2, inserts transparent bottle 4 in plate hole 2;

This transparent bottle 4 is made up of bottle 5 and bottle cap 6, be with cushion rubber 7 in the outside of bottle 5, substratum 9 be housed, in bottle cap 6, be provided with test paper carriage 8 in the inside of bottle 5, putting on this test paper carriage 8 has test paper 10, and these bottle cap 6 cover caps are on the bottleneck of transparent bottle 4.

The utility model has the advantages that: simple to operate, easy to use when detecting coliform, detected result is accurate, quick, and cheap, is easy to carry about with one and prolonged preservation, saves human and material resources and financial resources, has very strong practical value.

The utlity model has following accompanying drawing:

The disposable coliform detection kit of Fig. 1 the utility model schematic perspective view.

Fig. 2 A, B the utility model reaction grillage and Sptting plate schematic perspective view.

Fig. 3 A, the transparent bottle exploded view of B the utility model, assembly drawing.

Fig. 4 A, B, C the utility model reagent and dropper synoptic diagram.

Number in the figure is as follows:

1 detection kit, 2 plate holes, 3 anti-grillages

4 transparent bottle 5 bottles 6 bottle caps

7 cushion rubbers, 8 test paper carriages, 9 substratum

10 test paper, 11 thymolphthalein reagent, 12 eosin methylene blue agars

13 droppers, 14 Sptting plates, 15 dividing plates

Introduce embodiment below in conjunction with accompanying drawing:

See also shown in Fig. 1 and Fig. 2 A, the B, a kind of disposable coliform detection kit of the utility model, this detection kit 1 is a rectangle box body with cover, in box body, be provided with dividing plate 15, side at dividing plate 15 is equipped with reaction grillage 3, on reaction grillage 3, be provided with several plate holes 2, be inserted with transparent bottle 4 in each plate hole 2, form a Sptting plate 14;

See also shown in Figure 3, this transparent bottle 4 has bottle 5 and bottle cap 6, in the place of the upper end of each bottle 5 bottle mouth position less than bottle 5 one circles, be with a cushion rubber 7, bottle cap 6 is covered on bottleneck, plays airtight effect, substratum 9 is housed in the bottle 5, in bottle cap 6 above a protruding circular edge as test paper carriage 8, this test paper carriage 8 tops holder has test paper 10, and each transparent bottle 4 all can take off from reaction grillage 3.

See also shown in Figure 4ly, at the opposite side of detection kit 1 internal partition 15, putting has the bottle that thymolphthalein reagent 11 is housed, the bottle and the dropper 13 of eosin methylene blue agar 12.

When check is used, sample to be checked is diluted, get three extent of dilution then, a certain amount of sample diluting liquid in each extent of dilution is added in three transparent bottles 4, and with behind the dropper 13 sucking-off thymolphthalein reagent 11, drop in respectively on the test paper 10 of each bottle cap 6, then bottle cap 6 upright both lids on bottle 5 were sealed, be placed on and cultivate in 36 ℃ of incubators after 18-24 hour, observe liquid and the interior test paper change in color of bottle cap in the bottle 5.If the liquid yellowing is then positive for producing acid, it is negative for producing acid that liquid is red-purple; Look is the aerogenesis positive if test paper bleaches, and it is the aerogenesis feminine gender that test paper is blue look.On the flat board of eosin methylene blue agar 12, be seated in 36 ℃ of incubators after separation and Culture 18-24 hour producing the streak inoculation of sour aerogenesis male liquid, observe colonial morphology and microscopy, be intense violet color as bacterium colony, metalluster is arranged or be red-purple, microscopy is that the Gram-negative bactacin then can be confirmed as coliform-positive.Then, look into coliform group count (MPN) key, can report coliform numerical value (being the MPN value) according to coliform-positive bottle number.

Claims

1, a kind of disposable coliform detection kit, it is characterized in that: this detection kit is a rectangle box body with cover, in box body, be provided with dividing plate (15), side at dividing plate (15) is equipped with reaction grillage (3) and transparent bottle (4), and opposite side is being put the bottle that thymolphthalein reagent (11) are housed, the bottle and the dropper (13) of eosin methylene blue agar (12) respectively; Wherein:

This reaction grillage (3) is provided with plate hole (2), inserts transparent bottle (4) in plate hole (2);

This transparent bottle (4) is made up of bottle (5) and bottle cap (6), be with cushion rubber (7) in the outside of bottle (5), in the inside of bottle (5) substratum (9) is housed, in bottle cap (6), be provided with test paper carriage (8), this test paper carriage (8) is gone up to put test paper (10), and this bottle cap (6) cover cap is on the bottleneck of transparent bottle (4).