CN219533170U

CN219533170U - Mycoplasma detecting tube

Info

Publication number: CN219533170U
Application number: CN202320370393.XU
Authority: CN
Inventors: 何志昂; 姜维; 吴庆军
Original assignee: Dakewe Bio Engineering Co ltd
Current assignee: Dakewe Bio Engineering Co ltd
Priority date: 2023-02-20
Filing date: 2023-02-20
Publication date: 2023-08-15
Anticipated expiration: 2033-02-20

Abstract

The utility model discloses a mycoplasma detection tube, the top of a filter funnel is matched with a tube body, the top surface of the filter funnel is provided with a filter membrane, the bottom surface of the filter funnel is provided with an ultrafiltration membrane, negative pressure is formed in the filter funnel, negative pressure is formed in the tube body above the filter membrane, the tube body is provided with a diluent above the filter membrane, mycoplasma culture medium balls are fixedly arranged in the filter funnel, a plurality of mycoplasma detection primary antibodies are freely arranged in the filter funnel, and the appearance of each mycoplasma detection primary antibody is different in color. The vertical below that sets up at filter funnel of detection strip is equipped with a plurality of detection holes on the detection strip, sets up different kind mycoplasma detection secondary antibody on every detection hole. The puncture structure is arranged at the top of the detection strip. One end of the sampling tube is communicated with the inside of the tube body, and the other end of the sampling tube is provided with a sealing element. The utility model has simple structure, simple and convenient detection steps and accurate detection result, can detect various mycoplasma simultaneously, and improves the detection efficiency.

Description

Mycoplasma detecting tube

Technical Field

The utility model relates to the technical field of microbial culture, in particular to a mycoplasma detection tube.

Background

Mycoplasma is the smallest microorganism that survives extracellularly, a prokaryotic cell type of microorganism lacking a cell wall, is highly polymorphic, and has a variety of forms such as sphere, rod, wire, branch, etc. It is different from bacteria and also different from viruses. Mycoplasma widely exist in human and animal bodies, are mostly non-pathogenic, and are mainly mycoplasma pneumoniae, ureaplasma urealyticum, mycoplasma hominis, mycoplasma genitalium and the like. Mycoplasma can cause urogenital tract infection, mycoplasma pneumonia and the like, and the examination of whether a human body is infected by mycoplasma is an examination method which provides basis for clinical diagnosis and treatment of mycoplasma infection patients.

The biological samples for mycoplasma detection are typically urine, sputum, genital secretions, etc. The detection method comprises a culture method, enzyme-linked immunity, PCR, staining microscopic examination and the like. There are some problems in using the above detection method: 1. the consumable swab is not a closed environment during sampling, and the stored sample is easy to contact with other mycoplasma and bacteria contained in the air, so that the detection accuracy is easy to influence; 2. after sampling, the sample liquid is not diluted and filtered; 3. some detection methods can only detect one mycoplasma at a time, and if multiple mycoplasma are required to be detected, repeated detection is required, so that time and labor are consumed.

Disclosure of Invention

The present utility model aims to solve at least one of the technical problems existing in the prior art. Therefore, the utility model provides the mycoplasma detection tube, which can ensure the detection accuracy, detect a plurality of groups of mycoplasma and save the detection time.

The mycoplasma detection tube comprises a tube body, a filter funnel, a detection strip, a puncture structure and a tube cover. The top of filter funnel with body adaptation, the top surface of filter funnel is equipped with the filter membrane, the bottom surface of filter funnel is equipped with the milipore filter, form negative pressure in the filter funnel, the filter membrane is above form negative pressure in the body, the body in the top of filter membrane is equipped with the diluent, filter funnel internal fixation is equipped with mycoplasma culture medium ball, the free multiple mycoplasma that is equipped with in the filter funnel detects the primary antibody, every the mycoplasma detects the outward appearance colour of primary antibody different. The test strip is vertically arranged below the filter funnel, a plurality of test holes are formed in the test strip, and different mycoplasma test secondary antibodies are arranged on each test hole. The puncture structure is arranged at the top of the detection strip and is used for puncturing the side wall of the filter funnel. The pipe cover is arranged at the top of the pipe body, a suction pipe is arranged on the pipe cover, one end of the suction pipe is communicated with the inside of the pipe body, and the other end of the suction pipe is provided with a sealing element.

The mycoplasma detection tube provided by the embodiment of the utility model has at least the following beneficial effects:

by adopting the utility model of the embodiment, as the negative pressure environment is formed in the tube body above the filter funnel and the filter membrane, and the end part of the sampling tube is provided with the sealing element, the sampling tube can be ensured to be in a closed environment, other mycoplasma and bacteria in the air are prevented from entering the mycoplasma detection tube, the influence of external factors on the detection result is reduced, and the detection accuracy is ensured. During detection, a sealing element of the sampling tube is removed, the sampling tube is stretched into sample liquid to absorb the sample liquid under negative pressure, the sample liquid is mixed with and dissolved with impurities such as sputum through diluent, bacteria and impurities in the sample are removed through centrifugation of a filter membrane, and the detection accuracy is further improved; and centrifuging to remove most of liquid by using an ultrafiltration membrane, concentrating mycoplasma, providing nutrition for the mycoplasma by using a mycoplasma culture medium ball in a filter funnel, maintaining the biological activity of the mycoplasma, combining mycoplasma detection primary antibodies with different colors with corresponding mycoplasma detection secondary antibodies, performing immunoprecipitation aggregation in detection holes corresponding to detection strips to form color spots, and indicating the detection result. The utility model can realize aseptic sampling of mycoplasma, effectively filter bacteria and other microorganisms in sample liquid, concentrate sample volume, improve and maintain the concentration and biological activity of mycoplasma to be detected, and simultaneously identify various mycoplasma in the sample to be detected, and has the characteristics of simple structure, simple and convenient detection steps, accurate detection result and high detection efficiency.

According to some embodiments of the utility model, a waste liquid pipe is arranged below the filter funnel, the top of the waste liquid pipe is matched with the bottom of the filter funnel, the waste liquid pipe is communicated with the filter funnel, and negative pressure is formed in the waste liquid pipe.

According to some embodiments of the utility model, the piercing structure is provided with a pressing handle having a piercing tip at one end near the filter funnel and the other end extending outside the tube.

According to some embodiments of the utility model, a sample pad is provided at the upper end of the test strip, the sample pad is connected to the piercing tip, and a water absorbing pad is provided below the test hole of the test strip.

According to some embodiments of the utility model, the upper surface of the tube body is provided with an annular groove, in which the suction tube can be inserted.

According to some embodiments of the utility model, the mycoplasma culture medium pellet comprises a blue sphere and a layer of red mycoplasma solid medium attached to the outer surface of the blue sphere.

According to some embodiments of the utility model, the pore size on the filter is 0.45um.

According to some embodiments of the utility model, the ultrafiltration membrane has a pore size of 0.05um.

According to some embodiments of the utility model, the diluent is 0.1% dtt in PBS buffer.

According to some embodiments of the utility model, the mycoplasma culture medium pellet comprises 60% Difco PPLO broth (60%), horse serum (20%), 10% 15%yeast extreat solution,0.5% glucose, 0.1% L-arginine, 0.002% phenol red or bromophenol blue, 5% agarose, 0.5% urea.

Additional aspects and advantages of the utility model will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the utility model.

Drawings

The utility model is further described with reference to the accompanying drawings and examples, in which:

FIG. 1 is a first view of a Mycoplasma detection tube according to an embodiment of the present utility model;

FIG. 2 is a second view of a Mycoplasma detection tube according to an embodiment of the present utility model;

FIG. 3 is a schematic diagram showing the structure of a strip of a Mycoplasma detection tube according to an embodiment of the present utility model;

reference numerals:

101. a tube body; 102. a filter funnel; 103. a test strip; 104. a tube cover; 105. a filter membrane; 106. a dilution liquid; 107. an ultrafiltration membrane; 108. a mycoplasma culture medium pellet; 109. detecting the first antibody by mycoplasma; 110. a detection hole; 111. concentrating the liquid; 112. detecting the second antibody by mycoplasma; 113. a waste liquid pipe; 114. pressing a handle; 115. puncturing the tip; 116. a sample pad; 117. a water absorbing pad; 118. an annular groove; 119. a sampling tube; 120. and (3) a sample liquid.

Detailed Description

Embodiments of the present utility model are described in detail below, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to like or similar elements or elements having like or similar functions throughout. The embodiments described below by referring to the drawings are illustrative only and are not to be construed as limiting the utility model.

In the description of the present utility model, it should be understood that the direction or positional relationship indicated with respect to the description of the orientation, such as up, down, etc., is based on the direction or positional relationship shown in the drawings, is merely for convenience of describing the present utility model and simplifying the description, and does not indicate or imply that the apparatus or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present utility model.

In the description of the present utility model, a number means one or more, a number means two or more, and greater than, less than, exceeding, etc. are understood to not include the present number, and above, below, within, etc. are understood to include the present number. The description of the first and second is for the purpose of distinguishing between technical features only and should not be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated or implicitly indicating the precedence of the technical features indicated.

In the description of the present utility model, unless explicitly defined otherwise, terms such as arrangement, installation, connection, etc. should be construed broadly and the specific meaning of the terms in the present utility model can be reasonably determined by a person skilled in the art in combination with the specific contents of the technical scheme.

In addition, the technical solutions of the embodiments of the present utility model may be combined with each other, but it is necessary to be based on the fact that those skilled in the art can implement the technical solutions, and when the technical solutions are contradictory or cannot be implemented, the combination of the technical solutions should be considered as not existing, and not falling within the scope of protection claimed by the present utility model.

Referring to fig. 1 to 3, a mycoplasma detection tube according to an embodiment of the present utility model includes a tube body 101 and a filter funnel 102. Test strip 103, lancing structure, and cap 104. The top of filter funnel 102 and body 101 adaptation, the top surface of filter funnel 102 is equipped with filter membrane 105, the top of filter membrane 105 is equipped with diluent 106, the bottom surface of filter funnel 102 is equipped with milipore filter 107, be equipped with fixed mycoplasma culture medium ball 108 and free mycoplasma detection primary antibody 109 in the filter funnel 102, form the negative pressure in the body 101 more than filter funnel 102 and filter membrane 105, be equipped with fixed mycoplasma culture medium ball 108 and the mycoplasma detection primary antibody 109 of multiple different outward appearance colours in the filtration structure. The detection strip 103 is vertically arranged below the filter funnel 102, a plurality of detection holes 110 are correspondingly formed in the detection strip 103, and different mycoplasma detection secondary antibodies are arranged in each detection hole 110. A piercing structure is provided on top of the test strip 103 for piercing the sidewall of the filter funnel 102. The tube cover 104 is arranged at the top of the tube body 101, a suction tube 119 is arranged on the tube cover 104, one end of the suction tube 119 is communicated with the interior of the tube body 101, and the other end is provided with a sealing element.

By applying the utility model of the embodiment, as the negative pressure environment is formed in the tube body 101 above the filter funnel 102 and the filter membrane 105, and the end part of the sampling tube 119 is provided with the sealing element, the sampling tube can be ensured to be in a closed environment, other mycoplasma and bacteria in the air are prevented from entering the mycoplasma detection tube, the influence of external factors on the detection result is reduced, and the detection accuracy is ensured. During detection, the sealing element of the sampling tube 119 is removed, the sampling tube 119 is stretched into the sample liquid 120 to absorb the sample liquid 120 under negative pressure, the sample liquid 120 is mixed with and dissolved with impurities such as sputum through the diluent 106, bacteria and impurities in the sample are removed through the centrifugation of the filter membrane 105, and the detection accuracy is further improved; and then most of liquid is removed through centrifugation of an ultrafiltration membrane 107, mycoplasma is concentrated, a mycoplasma culture medium ball 108 in a filter funnel 102 provides nutrition for the mycoplasma and maintains biological activity of the mycoplasma, and after the mycoplasma detection primary antibodies 109 with different colors are combined with the mycoplasma detection secondary antibodies with corresponding types, the mycoplasma detection primary antibodies are subjected to immunoprecipitation aggregation in detection holes 110 corresponding to the detection strips 103 to form color spots with corresponding colors, and the detection results are indicated. The utility model can realize aseptic sampling of mycoplasma, effectively filter microorganisms such as bacteria in the sample liquid 120, concentrate the sample volume, improve and maintain the concentration and biological activity of mycoplasma to be detected, and simultaneously identify various mycoplasma in the sample to be detected, and has the characteristics of simple structure, simple and convenient detection steps, accurate detection result and high detection efficiency.

It will be appreciated that the tube may be incubated at a constant temperature for more than 24 hours at 37℃to further increase the concentration of mycoplasma.

Referring to fig. 1 and 3, it can be understood that a waste liquid pipe 113 is disposed below the filter funnel 102, the top of the waste liquid pipe 113 is adapted to the bottom of the filter funnel 102, and a negative pressure is formed in the waste liquid pipe 113. During the detection, impurities such as bacteria in the sample liquid 120 are filtered by the filter membrane 105, and most of the liquid in the sample liquid 120 flows to the waste liquid pipe 113 through the ultrafiltration membrane 107.

Referring to fig. 1-3, it will be appreciated that the piercing structure includes a crimping handle 114, the crimping handle 114 having a piercing tip 115 at one end adjacent the filter funnel 102 and extending outside the tube body 101 at the other end. Piercing tip 115, upon depression of handle 114, pierces the sidewall of filter funnel 102, such that mycoplasma-containing concentrate 111 in filter funnel 102 flows down test strip 103 and then through test aperture 110.

It will be appreciated that piercing tip 115 is made of a metallic material that is not susceptible to corrosion.

Referring to fig. 3, it can be appreciated that the sample pad 116 is disposed at the upper end of the test strip 103, the sample pad 116 is connected to the piercing tip 115, and the test strip 103 is disposed with a water absorbing pad 117 below the test hole 110. The concentrated solution 111 flows along the tip to the sample pad 116, and then flows through the detection hole 110 by chromatography of the water absorbing pad 117, and the sample pad 116 plays a role of drainage to prevent the concentrated solution 111 from dripping outside the detection strip 103.

Referring to fig. 1, it will be appreciated that the upper surface of the tube body 101 is provided with an annular groove 118, and that the suction tube 119 can be inserted into the annular groove 118.

It will be appreciated that the seal of sampling tube 119 is a frangible seal and that sample fluid 120 is aspirated upon removal of the frangible seal.

It will be appreciated that mycoplasma medium pellet 108 comprises a blue sphere and a layer of red mycoplasma solid medium attached to the outer surface of the blue sphere. If a large amount of mycoplasma grows normally, the red medium on the outer surface of the mycoplasma culture medium ball 108 falls off the skin to generate the internal blue color, so that a detector can primarily judge the growth of mycoplasma in the sample liquid 120.

It is understood that the pore size on the filter 105 is 0.45um.

It is understood that the pore size of the ultrafiltration membrane 107 is 0.05um.

It is understood that the diluent 106 is 0.1% DTT in PBS buffer.

It is understood that mycoplasma medium pellet 108 contains 60% Difco PPLO broth (60%), horse serum (20%), 10% 15%yeast extreat solution,0.5% glucose, 0.1% L-arginine, 0.002% phenol red or bromophenol blue, 5% agarose, 0.5% urea.

In the description of the present specification, reference to the terms "one embodiment," "some embodiments," "illustrative embodiments," "examples," "specific examples," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the utility model. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiments or examples. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

While embodiments of the present utility model have been shown and described, it will be understood by those of ordinary skill in the art that: many changes, modifications, substitutions and variations may be made to the embodiments without departing from the spirit and principles of the utility model, the scope of which is defined by the claims and their equivalents.

Claims

1. A mycoplasma detection tube, comprising:

the pipe body is provided with a plurality of holes,

the filter funnel, the top of filter funnel with body adaptation, the top surface of filter funnel is equipped with the filter membrane, the bottom surface of filter funnel is equipped with the milipore filter, form negative pressure in the filter funnel, form negative pressure in the body above the filter membrane, the body is equipped with the diluent in the top of filter membrane, filter funnel internal fixation is equipped with mycoplasma culture medium ball, the free multiple mycoplasma that is equipped with in the filter funnel detects first antibody, and every mycoplasma detects the colour of the outward appearance of first antibody different;

the detection strip is vertically arranged below the filter funnel, a plurality of detection holes are formed in the detection strip, and different mycoplasma detection secondary antibodies are arranged on each detection hole;

the puncture structure is arranged at the top of the detection strip and is used for puncturing the side wall of the filter funnel;

and the tube cover is arranged at the top of the tube body, a suction tube is arranged on the tube cover, one end of the suction tube is communicated with the inside of the tube body, and the other end of the suction tube is provided with a sealing element.

2. The mycoplasma detection tube of claim 1, wherein: the filter funnel is equipped with the waste liquid pipe below, the top of waste liquid pipe with filter funnel's bottom adaptation, the waste liquid pipe with filter funnel intercommunication, form the negative pressure in the waste liquid pipe.

3. The mycoplasma detection tube of claim 1, wherein: the puncture structure is provided with a pressing handle, one end of the pressing handle, which is close to the filter funnel, is provided with a puncture tip, and the other end of the pressing handle extends to the outside of the pipe body.

4. A mycoplasma detection tube according to claim 3, wherein: the upper end of the detection strip is provided with a sample pad, the sample pad is connected with the puncture tip, and the detection strip is provided with a water absorption pad below the detection hole.

5. The mycoplasma detection tube of claim 1, wherein: the upper surface of the tube body is provided with an annular groove, and the sampling tube can be embedded into the annular groove.

6. The mycoplasma detection tube of claim 1, wherein: the mycoplasma culture medium ball comprises a blue sphere and a layer of red mycoplasma solid culture medium attached to the outer surface of the blue sphere.

7. The mycoplasma detection tube of claim 1, wherein: the pore size on the filter membrane was 0.45um.

8. The mycoplasma detection tube of claim 1, wherein: the aperture of the ultrafiltration membrane is 0.05um.

9. The mycoplasma detection tube of claim 1, wherein: the dilution was 0.1% dtt in PBS buffer.