CN206014922U - A kind of high flux micro algae growth test device - Google Patents

Abstract

This utility model provides a kind of high flux micro algae growth test device, and device includes cultivating support body, light source, CO₂Or air source of the gas, numeric type gas flowmeter, milliliter level culture bottle, many valve parallel regulators of gas and pneumatic filter；The culture support body is provided with the location structure that several are used for accommodating the milliliter level culture bottle.Microalgae high flux of the present utility model grows test device, it is little which not only remains system, collimation is high, many advantages, such as applied range, also done in terms of high flux, motility, stability again and broken through, realize that 1 cubic meter can at most carry out the high flux of 1200 groups of reaction culture bottles and arrange, greatly save search time.Can achieve the precise control that illumination and supply is carried out to single reaction culture bottle；The risk of pollution can be accurately sampled and can be reduced by syringe in-situ sampling, realized that culture overall process reaction system is constant, lifted the accuracy of result.

Description

A kind of high flux micro algae growth test device

Technical field

This utility model is related to the culture of photosynthetic microorganism and detection technique field, is specifically related to a kind of high flux microalgae Growth test device and method.

Background technology

The culture test of microalgae is a requisite ring in algology research, in microalgae bioenergy, ecotoxicology, In the researchs of various fields such as body eutrophication, water body plankton community structure, general by arrange Normal group with Treatment group (such as adds the poisonous and harmful elements such as heavy metal；Add the high sewage containing N, P amount；Add various dose nutritive salt；Add Different algae kinds；Add different planktons etc.), compare the growing state of two groups of frustules, determine algae biomass, oils and fatss pigment and contain The important evaluation indexes such as amount, photosynthetic efficiency, culture fluid composition, provide data supporting for research conclusion.At present generally general micro- Algae cultural method is as culture vessel with conical flask, controls cultivation temperature and optical density by constant temperature illumination box, realizes Microdisk electrode.During sampling, need to operate in aseptic operating platform, to reduce the probability of bacterial infection.

It is well known that in biotic experiment, generally require to react microorganism or cell growth characteristics, ecotoxicological into Row test, especially for the screening of mutant.In general microorganism or cell are tested, orifice plate, orifice plate can be used Specification have 6,12,24,48,96 holes, the volume in each hole is 16.8ml, 6.9ml, 3.4ml, 1.7ml, 0.36ml successively.? In the growth characteristics test of general biological or cell, toxic reaction test experiments, orifice plate has orthobaric volume, growth area etc. Specification is consistent, can disposably do several groups to tens groups parallel laboratory tests, with higher test flux；And each Kong Pei of orifice plate Foster system is little, the problems such as will not wasting excessive culture medium and biomaterial and expend more incubation time, is consequently adapted to Standardized assay is operated, and makes contrastive experimental data also relatively reliable, objective.

However, conical flask but has a lot of deficiencies with the cultivating system of orifice plate ware：Such as the sources of the gas such as carbon dioxide cannot be supplied, The dispersion of cultivating system cell is uneven, and the speed of growth is slow；Culture space is easily restricted, and for tens of milliliters of systems, unit is empty Between arrange group limited amount, i.e. flux low；Each group light density is easily uneven, and collimation is poor；Sampling is loaded down with trivial details, and easy infection is thin Bacterium, cultivating system are not sufficiently stable etc..Not only sensitivity is not high, is also consumed by plenty of time cost, and it is now right much to meet Scientific research demand in microalgae cell.Such as 103074205 B of Chinese patent Authorization Notice No. CN, although which also reveal that one kind " high-throughput screening method of a kind of electric turntable system and microalgae high flux screening device and microalgae ", but which is substantially still First microalgae is inoculated in microwell plate, then by microwell plate tabular draw-in groove 3, rotates rotating disk, cultivated under illumination, micro- after measurement culture Absorbance is converted into corresponding microalgae cell dry weight concentrations, screens microalgae with this by the absorbance in orifice plate per hole.The practicality is new Though type solves the problems, such as illumination and shaking flask, for the CO needed for microalgae cell growth₂, refer only in text, in inoculation microalgae The CO of 2-50% volume is first encapsulated to before microwell plate₂In microwell plate；But microalgae cell is difficult to tolerate such high concentration CO₂, also cannot discharge the O for hindering photosynthetic reactor in time₂, so cannot also meet test, the requirement of screening.While the disclosure Patent formula in, in each hole, culture amount is too little.Only the algae solution of 0.1ml, is not enough to support such as dry mass, photosynthesis The measurement of a lot of important parameters such as efficiency, culture fluid TOC, TN Organic substance Changing Pattern.Which is mainly based upon the culture of 96 orifice plates System, single Kong Weiyi culture unit, and whole plate are one, and independence is poor.In terms of being mainly manifested in three below, its One, in single hole, carbon dioxide flexibly can not be controlled, and be difficult to arrange gradient between same plate porous；Two, single culture is single Unit is unable to complete independently side culture frontier inspection and surveys operation, once sampling, in culture hole, carbon dioxide will change, even if sealing again Enter carbon dioxide, also not parallel with other holes, it is impossible to carry out parallel control experiment again.Additionally, cultivating in the patent single First shape is immutable, it is impossible to for studying reactor shape for the impact of light utilization ratio.

Except above-mentioned traditional cultural method, some novel photo-biological reactors are also applied to Microalgae biotechnology in recent years Research field.These new reactor skewed popularities are different, the flexible control for laying particular emphasis on condition of culture having, such as flexibly choosing Light source is selected, various sources of the gas and different aeration modes can be input into, can control ambient temperature etc.；The efficient profit for laying particular emphasis on light having With by arranging reflex reflector, the light-receiving area of raising reactor and light reception intensity；Had lays particular emphasis on reactor closure, with One aseptic culture environment is provided.The results for being then designed as more conducively culture having, etc..These new photocatalysis, Bioreactor is with the obvious advantage, for research micro algae growth, reacts helpful, is also more and more used for microalgae biological Research field.

But these bioreactors do not embody the importance of flux, can only generally arrange several groups, more than ten groups Reaction member, and cultivating system is larger.With the fast development of Microalgae biotechnology, microdisk electrode is tested in ecotoxicology, The multiple fields such as body eutrophication, microalgae bioenergy are more widely applied, and the reactor of small throughput can greatly increase and grind The time cost that studies carefully, hinders the fast development of Microalgae biotechnology.And current Photoreactor is done in terms of collimation A lot of work, but have ignored the flexible modulation of single reactor unit, are not enough to support experimentation more complicated and changeable.

Utility model content

Technical purpose of the present utility model is to provide a kind of high flux growth for being suitable to microalgae and test device.Which is not only protected Stay system little, collimation is high, many advantages, such as applied range, also done in terms of high flux, motility, stability again prominent Broken, realize that 1 cubic meter can at most carry out the high flux of 1200 groups of reaction culture bottles and arrange, greatly save search time.Can be real The precise control of illumination and supply is now carried out to single reaction culture bottle, helps carry out increasingly complex changeable experimentation；Logical The risk that syringe in-situ sampling can accurately be sampled and can reduce pollution is crossed, is realized that culture overall process reaction system is constant, is carried Rise the accuracy of result.This utility model can promote Microalgae biotechnology fast-developing so as to be applied to more scientific domains.

For reaching above technical purpose, the technical solution of the utility model is：

A kind of high flux micro algae growth test device, including cultivating support body, light source, CO₂Or air source of the gas, numeric type gas Effusion meter, milliliter level culture bottle, many valve parallel regulators of gas and pneumatic filter；The culture support body is provided with several For accommodating the location structure of the milliliter level culture bottle.

Wherein, the milliliter level culture bottle has porous bottle stopper or porous threaded cap, is plugged with the hollow glass of grade in hole Glass airway and/or silica gel tube.The light source is preferably LED, fluorescent lamp, mercury lamp, electric filament lamp, electricity-saving lamp.

Wherein, can also include that the both sides of a light source and supply regulating box, the light source and supply regulating box have to open Mouth side；The light source that can be emitted beam to two open sides is set in middle part, fixed culture is set in the left and right inwall of open side The supporting construction of support body；Light source and supply regulating box and CO₂Or the connection of air source of the gas, it is provided with source of the gas regulating valve, light source and supply Regulating box is provided with light source intensity adjusting knob.

Wherein, the culture support body includes single porous support body or double staggeredly hole culture support body, can place in each hole Culture bottle less than 100ml.

Wherein, culture support body (the constituting a culture unit) includes single porous support body or double staggeredly hole culturing rack Body, constitutes (material is made every effort to overcome in Asia, 4 parts) by biside plate and upper and lower plate, in front and back both-side opening；Long 50cm, wide 8cm；Upper plate is big Bore dia：3.7cm, totally 10 hole, from left to right between hole interval 1cm (reaction member is spaced 1cm to reduce screening to each other to light Gear), lower plate small-bore 1cm.The culture bottle less than 100ml (such as 50ml, 30ml, 10ml) can be placed in each hole；The culture The tapered bottom in bottom of bottle portion, the tip of its conical lower portion are pointed to the aperture of the lower plate.The specification of single porous support body is long * Wide * is high：0.5*0.2*0.5 rice.Adaptation 50ml, 3.5cm optical path culture bottle, optical path very little, light transmittance are high, micro algae growth speed Hurry up, shorten search time.3.5cm optical path culture bottles front and rear row can staggeredly avoid light source from being blocked illumination by previous row's culture bottle.

Wherein, the porous bottle stopper or porous threaded cap of the milliliter level culture bottle includes at least 3 holes, in one of hole Sealing is cased with micrometer grade hole footpath silica gel tube or the silica gel tube with one section of self-closing flexible pipe, and silica gel tube outer end is arranged sampling end Mouthful, it is preferred that sampling port is the syringe needle of sampling injector, need not open culture bottle and just easily can sample, it is possible to decrease culture The risk that bottle internal medium is contaminated；Grade double glazing airway, many valve shuntings of connection gas is plugged with another hole Actuator；3rd hole is with the bacteriological filter film sealing of nanoscale aperture, it is ensured that prevent while culture bottle inside and outside air pressure balance The pollution of the heterologous organisms such as antibacterial.

Wherein, supervisor one end of many valve parallel regulators of the gas passes through hose connection CO₂Or air source of the gas, outside supervisor Side radial distribution arranges several isocons with separate valve, these isocons and a rubber tube socket, this rubber Leather hose is connected with the grade double glazing airway on milliliter level culture bottle again.The many valve parallel regulators of gas can ensure each The CO of milliliter level culture bottle₂The uniformity of supply, improves the reliability of collimation experiment, and each single culture bottle is controllable Property.

Wherein, the pneumatic filter is arranged on CO₂Or between air source of the gas and many valve parallel regulators of gas.Gas mistake The setting of filter is preferably：The box body of circle is set on the rubber tube of supply, and box body is built with pneumatic filter：Rubber tube one End connection source of the gas, one end is connected to the grade double glazing air supply pipe on culture bottle.The box body can be divided into two and can tear open The lid for dividing, installs a micropore filter paper between two lids.

High flux micro algae growth test device of the present utility model, can also include plate of low-pass optical optical filter, the low pass Optical filter is located at outside milliliter level culture bottle.Preferably, plate of low-pass optical optical filter makes cylindrical shape, is enclosed in the training of milliliter level On the outside of foster bottle.

Technique effect and technical advantage acquired by this utility model be：

(1) high flux property：In existing other common microalgae high flux micro algae growth test devices, each culture unit The cultivating system of (constituting a culture unit when cultivating and filling culture bottle on support body) reaches upgrading, and reaction member generally only has Several (5-6).Each culture reaction system is substantially reduced in this utility model to milliliter level, by cultivating support body, is set again Reaction member spatial classification is put for vertical three row or horizontal double-row etc., single reaction member spacing >=1 centimetre is set, height is can achieve Flux culture is tested.

For example, if the long * width * of reactor entirety specification is high：0.5*0.2*0.5 rice, takes 0.05 cubic metre, for 50ml's Cultivating system, can arrange 60 groups of Duplicate Samples, substantially meet more than 99% experiment demand.If with LED point lamps as light source, 1 stands 20 groups of reactor units can at most be built by square rice, at most can arrange 1200 groups of Duplicate Samples simultaneously.For be less than 50ml such as 30ml, The cultivating system of 10ml, can also arrange more multigroup, and each sample all can individually control to ventilate by many valve parallel regulators of gas Amount, and light quantum density can be adjusted by plate of low-pass optical optical filter, with very strong motility.Compared to mesh in terms of flux Front micro algae culturing device, improves several magnitudes.Applied range, can apply to microalgae bioenergy, ecotoxicology, The various fields such as body eutrophication, water body plankton community structure.

(2) search time shortens：Reaction system is narrowed down to several to tens milliliters, amount of samples is saved；And it is less Optical path, can greatly promote the frustule speed of growth, and search time can also shorten.

(3) flexible modulation：Existing many flux Photoreactors do not have control measures, this reality for single reactor unit With new milliliter level culture bottle specification plurality of optional, shape such as cylinder, square plurality of optional；By many valve parallel regulators of gas Can independent control ventilation to each culture bottle；Light source arbitrarily can be selected, and such as LED or fluorescent tube all mate very much；Logical Cross the actual light quantum density that optical filter can individually control each culture bottle.

(4) collimation reliability is high：Symmetry principle setting is pressed in culture bottle position, each culture bottle light of each culture support body Controllable with gas basically identical.

(5) culture environment stability：Existing Photoreactor sampling mode is that open type and bottom leaky, this utility model are adopted Special porous vice realizes in-situ sampling with reference to syringe, increases system aseptic performance, it is ensured that reaction system is stable.Sterilizing training After foster bottle is used for culture, air filter is added to ensure gnotobasiss using porous vice, a hole is with grade double glazing Pipe provides source of the gas as airway to culture fluid, and another hole insertion micron order aperture silica gel tube is soft with one section of self-closing The silica gel tube of pipe, the termination of silica gel tube are available for being connected a sampling injector or negative pressure bag, using syringe or negative pressure during sampling Bag sampling, it is convenient not only to sample, moreover it is possible to reduce the pollution that open type sampling brings, so as to ensure whole cultivation period culture environment Stable.Wherein the 3rd hole is with the bacteriological filter film sealing of nanoscale aperture, it is ensured that prevent while normal atmosphere (An) in culture bottle The pollution of the heterologous organisms such as antibacterial, is advantageous to the strict experimentation of sterility requirements.

(6) for 103074205 B of patent CN, under same medium, intensity of illumination, initial inoculation concentration, Due to may be flowed into carbon dioxide, rather than encapsulation CO2 enters before the reaction, therefore can provide carbon source and stirring action, maximum Biomass can reach 4.2 and 4.8g/l, improve more than 400%, under the conditions of same optical path, can also be higher.This device is being remained While high flux advantage, with bigger cultivating system (1ml-100ml), more test samples can be provided and be simultaneously used for giving birth to Thing amount, photosynthetic efficiency, culture fluid TOC, TN Organic substance Changing Pattern, zwitterion, Metal Ions Absorption efficiency, circulation training Measure of many indexes such as foster base action effect etc., are not limited to Strain selection, can also be applied to microalgae bioenergy, ecological The various fields such as toxicology, body eutrophication, water body plankton community structure.Also by many valve shuntings of gas in this device Actuator to each culture bottle can independent control ventilation, and each culture bottle be completely self-contained, culture bottle adopt Special porous vice, a hole provide source of the gas using grade double glazing pipe as airway to culture fluid, and another hole insertion is micro- Meter level pore size silica gel pipe, tube head are connected syringe, using syringe sampling, can sample any time, no in incubation There can be any interference to culture environment.It can also be square and other shapes that this device culture bottle can be circle, Ke Yiyong In research reactor shape for the utilization ratio of light.

Description of the drawings

Knots of the Fig. 1 for the high flux micro algae growth test device (without supply regulating box) of this utility model preferred embodiment Structure schematic diagram；

Structural representations of the Fig. 2 for the supply regulating box with light source of embodiment illustrated in fig. 1；

Fig. 3-Fig. 5 is that culture support body of the present utility model is embodied as that single layer arranging, monolayer be double, Multi-layer single-row structure is shown It is intended to；

Structural representations of the Fig. 6 for the milliliter level culture bottle of this utility model embodiment illustrated in fig. 1；

The structure of perforated rubber stoppers of the Fig. 7 for combining on the milliliter level culture bottle of this utility model embodiment illustrated in fig. 1 is shown It is intended to；

Gas many valve parallel regulator structural representations of the Fig. 8 for this utility model embodiment illustrated in fig. 1；

Skies positioned at air inlet pipe and gas many valve parallel regulator between of the Fig. 9 for this utility model embodiment illustrated in fig. 1 The structural representation of air filter；

Figure 10 is that the syringe construction that this utility model embodiment illustrated in fig. 1 is connected with the sampling port of sampling silica gel tube shows It is intended to；

In figure：

11- sources of the gas；12- silica gel catheters；13- air filters；14- numeric type gas flowmeters；The many valve shuntings of 15- gases Actuator；16- expanded rubber plugs：17- double glazing airways；18- glass milliliter level culture bottles；19- cultivates support body；On 191- The macropore of plate；The aperture of 192- lower plates；20- sampling injectors；21- tubular type light sources；

22- supplies regulating box；221- culturing rack support structures；A- cultivates support body upper plate, and B- cultivates support body lower plate；151- Air inlet；152- air-guide main tubes road；153- isocons；154- flow dividers；131- air filters are taken over；132- filters； The interchangeable sampling syringe needles of 201-.

Specific embodiment

Following examples further illustrate content of the present utility model, but should not be construed as to restriction of the present utility model. In the case of without departing substantially from this utility model spirit and essence, modification that this utility model method, step or condition are made or Replace, belong to scope of the present utility model.

If not specializing, the conventional meanses that technological means used are well known to those skilled in the art in embodiment.

In conjunction with shown in Fig. 1-2, a kind of high flux micro algae growth test device, including illumination and supply regulating box 22, in case It is provided with culture support body 12, light source 21, many valve parallel regulators 15, CO of milliliter level culture bottle 18, gas₂Or air source of the gas 11 with And pneumatic filter 13, the pneumatic filter 13 is also connected with numeric type gas flowmeter 14, enters into many valves of gas to monitor The gas flow of parallel regulator 15.It is described as follows：

1) light source and supply regulating box 22 (as shown in Figure 2)：Its both sides has open side；Arranging in middle part can be to two The tubular type such as LED that open side emits beam light source 21, in the support that the left and right inwall of open side can arrange fixed culture support body Structure 221；Light source and supply regulating box 22 and CO₂Or air source of the gas 11 connects, source of the gas regulating valve and gas flowmeter 14 is provided with (such as Fig. 1), light source and supply regulating box 22 are provided with LED lamp tube light intensity regulating button (not shown).Wherein light source and supply regulating box 22 Main Function be and the adjusting knob etc. of installing light source and feeder for fixed light source and feeder.Therefore, light source Structure is not required with supply regulating box.

2) culture support body (is cultivated support body and internal fixed culture bottle constitutes one and cultivates unit) as in Figure 3-5:Single The single or monolayer row porous support body of layer (such as Fig. 3, shown in 4) or Multi-layer single-row culture support body (as shown in Figure 5), are by biside plate Upper and lower with least one set plate A, B constitute (material is made every effort to overcome in Asia, 4 parts), in front and back both-side opening；Long 50cm, wide 8cm；Upper plate A 191 diameter of macropore：3.7cm, totally 10 hole, from left to right between hole interval 1cm (reaction member is spaced 1cm to reduce to each other to light Block), 192 footpath 1cm of lower plate B aperture.Per group of upper Centered macropore 191 of lower opening is interior with aperture 192 can place one little Glass milliliter level culture bottle 18 in 50ml (such as 30ml, 10ml).3.5cm optical path culture bottles, by row porous support body 19 The macropore 191 of a row interlocks/misplaces with the front and rear row of the macropore 191 of previous row afterwards, can avoid light source by previous row's glass milliliter Level culture bottle 18 blocks illumination.

For, shown in Fig. 3, the specification of the porous support body 19 of single monolayer is that long * width * is high：0.5*0.2*0.5 rice.Adaptation 50ml, 3.5cm optical path culture bottle, optical path very little, light transmission rate are high, and micro algae growth speed is fast, can shorten search time.

3) glass milliliter level culture bottle 18 (as shown in Figure 6,7)：Glass milliliter level culture bottle 18, the porous that bottleneck is provided with Rubber stopper or threaded cap 16, it include that at least 3 holes, expanded rubber plug 16 as shown in Figure 7, one of hole interior sealing are cased with One micron order aperture silica gel tube 162 or the silica gel tube 162 of the flexible pipe with the self-closing of one section of energy, the outer end of silica gel tube 162 can For being arranged the syringe needle 201 of a sampling injector 20, the syringe needle 201 is connected all the time with silica gel tube 162, or is only faced in sampling When connect, syringe needle 201 is available for sampling injector 20 to connect；During sampling, draw syringe 20 to form negative pressure, culture bottle 18 need not be opened (as shown in Fig. 1 and Figure 10) just easily can be sampled, and the internal medium of controllable culture bottle 18 is not easily susceptible to pollute；Another hole Grade double glazing airway 161 is plugged with, an isocon 153 for connecting many valve parallel regulators 15 of gas is available for；Porous 3rd hole (Fig. 7 is shown in by schematic diagram) of rubber stopper 16, is with the bacteriological filter film sealing of nanoscale aperture, balances normal in culture bottle The pollution of the heterologous organisms such as antibacterial is prevented while atmospheric pressure.

In other embodiments, micron order aperture silica gel tube is can be replaced with one section of self-closing flexible pipe Silica gel tube 162, in 162 bottom of silica gel tube insertion culture bottle 18；In sampling, filled using other negative pressure bags or similar negative pressure Put closed with silica gel tube 162 dock, the release negative pressure device such as negative pressure bag can achieve automatic sampling, and sampling is directly pulled out after terminating Negative pressure bag, the self-closing hose segment of silica gel tube will close sealing automatically, realize sterile working.

Additionally, culture bottle 18 itself can be fabricated to using the transparent material of extrudable deformation, such as PC PVC etc., can both expire The demand of sufficient light autotrophic microbe, when can sampling again, direct handss hold culture bottle extruding, make sample liquid wash micrometer grade hole open The silica gel tube 162 in footpath, overflows sample liquid.After extruding is unclamped, sample liquid flows back, and 162 tube wall of silica gel tube in micron order aperture is sticked automatically Close closing, it is also possible to realize sterile working.

4) gas many valve parallel regulator (as shown in Figure 8) (provides CO2 gases to micro- with many valve parallel regulators of gas Algae) 152 one end of air-guide main tube road air inlet 151 by hose connection CO2 or air source of the gas (see Fig. 1 upper lefts), inducing QI master The homogeneous radiation shape of both sides axially outside of pipeline 152 is distributed several isocons 153 for being provided with separate valve 154, these points Flow tube and the socket of rubber tube, this rubber tube again with milliliter level culture bottle 18 on grade double glazing airway 161 Connection.The many valve parallel regulators 15 of gas can ensure the uniformity of the CO2 supplies of each milliliter of level culture bottle 18, improve collimation The reliability of experiment, and the controllability of each single culture bottle 18.

5) as shown in figure 9, arranging air filter 13 on the rubber pipeline of supply, which includes that two face each other face phase The leak shape air filter adapter 131 of button, and sandwiched filter 132 again between two air filter adapters 131, institute Filter 132 is stated comprising at least one of which micropore filter paper.

6) plate of low-pass optical optical filter：

Cylindrical shape is made, the outside of milliliter level culture bottle 18 can be enclosed in.

High flux micro algae growth test device of the present utility model, is specific to the culture and test of microalgae and designs. If single reactor unit (is cultivated support body 19 and constitutes a reactor when a milliliter level culture bottle 18 is fixed in each hole Unit) the long * width * height of entirety specification：0.5*0.2*0.5 rice, takes up room 0.05 cubic metre.Using vertical three row, often 10 are arranged Hole, horizontal double-row parallel arrangement mode, for the cultivating system of 50ml, can arrange 60 groups of Duplicate Samples.Reactor is with LED as light Source, 1 cubic metre can at most be built 20 groups of reactor units, at most can arrange 1200 groups of Duplicate Samples simultaneously.And for less than 50ml Such as 30ml, the cultivating system of 10ml can also be more；It is logical that each sample can all pass through the independent control of many valve parallel regulators 15 of gas Tolerance, and light quantum density can be adjusted by plate of low-pass optical optical filter, with very strong motility.

Using high flux micro algae growth test device of the present utility model, different algaes can be set in the experiment of same batch Multiple realities such as cell initial concentration, different ventilations, different optical density gradients, Different Nutrition factor concentration, physical and chemical factor test Research is tested, search time is greatly shortened.High flux micro algae growth test device can also accomplish aseptic culture, wherein special three Cellular type rubber stopper 16 or vice (bottle cap) seal milliliter level culture bottle 18, and a hole is inserted double glazing airway 161 and connects sky Air filter, another hole are connected the syringe needle 201 of syringe with micron order aperture silica gel tube 162, and sampling in this way avoids uncapping The pollution that formula sampling brings, it is ensured that the aseptic of cultivating system, for milliliter level cultivating system, tight sterility requirements are more Seem most important；High flux culture test device of the present utility model also supports that access carbon dioxide, oxygen etc. are various simultaneously Source of the gas.

The technique effect of above-mentioned high flux micro algae growth test device is：

(1) supply of CO2 can be set according to gas flowmeter on demand, the O2 produced in reactor, balance can be discharged Inner and outer air pressure, cultivating system cell disperse evenly；

(2) cultivating system of single culture bottle is little, and in system, micro algae growth speed is fast, and testing efficiency is high, and sensitivity is preferable, Substantial amounts of experimental period can be saved；

(3) illumination condition and controllable Supplying gas condition of average optical are provided, and the collimation of experiment is preferable；

(4) sample tap is carried, and convenient sampling is difficult bacterial infection, and cultivating system is stable；

(5) unit space setting is multigroup, with high flux property；

(6) each culture bottle (each culture bottle), can flexible modulation.

The above is only preferred implementation of the present utility model, it is noted that for the common skill of the art For art personnel, on the premise of without departing from this utility model know-why, some improvements and modifications can also be made, these change Enter and retouch also to should be regarded as protection domain of the present utility model.

Claims (10)

1. a kind of high flux micro algae growth test device, including cultivating support body, light source, CO₂Or air source of the gas, numeric type gas stream Gauge, milliliter level culture bottle, many valve parallel regulators of gas and pneumatic filter；The culture support body is provided with several use In the location structure for accommodating the milliliter level culture bottle.

2. high flux micro algae growth test device according to claim 1, it is characterised in that：The milliliter level culture bottle tool There are porous bottle stopper or porous threaded cap, grade double glazing airway and/or sampling silica gel tube is plugged with hole.

3. high flux micro algae growth test device according to claim 1, it is characterised in that：The light source is LED, glimmering Light lamp, mercury lamp, electric filament lamp, electricity-saving lamp.

4. high flux micro algae growth test device according to claim 1, it is characterised in that：Also include a light source and confession Gas regulating box, its both sides have open side, and the culture support body is assembled in the light source and supply regulating box；In middle part The light source that can be emitted beam to two open sides is set, in the supporting construction that the left and right inwall of open side arranges fixed culture support body； Light source and supply regulating box and CO₂Or air source of the gas connects and be provided with source of the gas regulating valve, light source and supply regulating box are additionally provided with light Source light intensity regulating button.

5. high flux micro algae growth test device according to claim 1, it is characterised in that：The culture support body includes list Row's porous support body or double staggeredly hole culture support body, the location structure for accommodating the milliliter level culture bottle is to be arranged at the culture Several holes on support body, can place the culture bottle less than 100ml in each hole.

6. high flux micro algae growth test device according to claim 5, it is characterised in that：The culture support body includes setting There are lower plate and a upper plate in hole, upper panel aperture is more than lower panel aperture, the tapered bottom in the culture bottle bottom, the point of its conical lower portion End is pointed to the hole of the lower plate.

7. high flux micro algae growth test device according to claim 2, it is characterised in that：The milliliter level culture bottle Porous bottle stopper or porous threaded cap include that at least 3 holes, one of hole interior sealing are cased with micrometer grade hole footpath silica gel tube or carry The silica gel tube of one section of self-closing flexible pipe, the silica gel tube outer end are arranged sampling port；Grade is plugged with another hole hollow Glass airway, many valve parallel regulators of connection gas；3rd hole is with the bacteriological filter film sealing of nanoscale aperture, it is ensured that training The pollution of the heterologous organisms such as antibacterial is prevented while foster bottle inside and outside air pressure balance.

8. high flux micro algae growth test device according to claim 2, it is characterised in that：The many valve shuntings of the gas are adjusted Supervisor one end of section device passes through hose connection CO₂Or air source of the gas, on the outside of supervisor, radial distribution arranges several with independent The isocon of valve, these isocons and the socket of rubber tube, this rubber tube again with milliliter level culture bottle on grade Double glazing airway connects.

9. high flux micro algae growth test device according to claim 1, it is characterised in that：The pneumatic filter is arranged In CO₂Or between air source of the gas and many valve parallel regulators of gas.

10. high flux micro algae growth test device according to claim 1, it is characterised in that：Also include that plate of low-pass optical is filtered Mating plate, the plate of low-pass optical optical filter are located at outside milliliter level culture bottle.