CN107177486A - Streptococcus suis culture inactivation integrated bottle - Google Patents

Streptococcus suis culture inactivation integrated bottle Download PDF

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Publication number
CN107177486A
CN107177486A CN201710474995.9A CN201710474995A CN107177486A CN 107177486 A CN107177486 A CN 107177486A CN 201710474995 A CN201710474995 A CN 201710474995A CN 107177486 A CN107177486 A CN 107177486A
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CN
China
Prior art keywords
bottle
streptococcus suis
valve
integrated
inactivator
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201710474995.9A
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Chinese (zh)
Inventor
丁刚强
梁远雄
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guangxi Assured Source Biotechnology Co Ltd
Original Assignee
Guangxi Assured Source Biotechnology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guangxi Assured Source Biotechnology Co Ltd filed Critical Guangxi Assured Source Biotechnology Co Ltd
Priority to CN201710474995.9A priority Critical patent/CN107177486A/en
Publication of CN107177486A publication Critical patent/CN107177486A/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/08Flask, bottle or test tube
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/38Caps; Covers; Plugs; Pouring means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02Stirrer or mobile mixing elements

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  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a kind of Streptococcus suis culture inactivation integrated bottle, including bottle, the bottle top is provided with bottle cap, the bottle bottom, which is provided with base, the bottle cap, is provided with inactivator residual chamber, and the inactivator residual chamber is connected by pipeline with the bottle, level is provided with valve on the bottle cap, pipeline described in the valve blocks, the inactivator residual chamber top is provided with closure, and the base is provided with agitating device.Streptococcus suis culture inactivation integrated bottle disclosed by the invention can instill inactivator in bacterium solution on the premise of need not opening bottle cap, greatly reduce the contaminated probability of bacterium solution in inactivation process, improve the purity of vaccine, be that raiser adds income.

Description

Streptococcus suis culture inactivation integrated bottle
Technical field
The present invention relates to a kind of Spawn incubation bottle, specially a kind of Streptococcus suis culture inactivation integrated bottle belongs to biological section Skill application field.
Background technology
Streptococcus suis is generally cultivated in the laboratory of large―scale hoggery plant, for making Streptococcus suis inactivated vaccine, Streptococcus suis needs to be used for making vaccine by inactivation after culture knot speed, and universal ablation method is now by blake bottle Bottle cap open, then inactivator is instilled in blake bottle, this process is easily caused the bacterium in environment into blake bottle, shadow The purity of strain in blake bottle is rung, and then influences the effect of vaccine, serious results even in pig death.
The content of the invention
The purpose of the present invention, which is that, provides a kind of Streptococcus suis culture inactivation integrated bottle in order to solve the above problems.
The present invention is achieved through the following technical solutions above-mentioned purpose, and a kind of Streptococcus suis culture inactivates integrated bottle, including Bottle, the bottle top is provided with bottle cap, and the bottle bottom, which is provided with base, the bottle cap, is provided with inactivator residual chamber, institute State inactivator residual chamber to connect with the bottle by pipeline, level is provided with valve on the bottle cap, described in the valve blocks Pipeline, the inactivator residual chamber top is provided with closure, and the base is provided with agitating device.
Preferably, level is provided with valve socket on the bottle cap, and the valve is provided with through hole, the through hole and the pipe Road is corresponding.
Preferably, the left and right sides of pipeline is provided with ground glass on the bottle cap, and the valve socket is frosted mouthful, described Valve exterior surface is provided with frosted, and the diameter of the through hole is less than the diameter of the pipeline.
Preferably, the splash bar device includes motor, rotor and stirring rod, and the rotor is arranged on the motor, institute Stirring rod is stated to be fixed on the rotor.
Preferably, the rotor is to be provided with screw-casing hole in the middle of cylinder, the rotor.
Preferably, the stirring rod is fold-line-shaped, and the stirring rod bottom is linear pattern borded pile.
Preferably, the screw-casing hole matches with the borded pile.
Preferably, the base is provided with battery, and the battery is connected with the motor.
Preferably, the closure is sheet rubber.
The beneficial effects of the invention are as follows:Streptococcus suis culture inactivation integrated bottle disclosed by the invention can need not open bottle Inactivator is instilled in bacterium solution on the premise of lid, the contaminated probability of bacterium solution in inactivation process is greatly reduced, improves vaccine Purity, be that raiser adds income.
Brief description of the drawings
Fig. 1 is overall structure diagram of the invention.
Fig. 2 is the structural representation of rotor of the present invention.
Fig. 3 is the structural representation of stirring rod of the present invention.
Fig. 4 is the structural representation of valve of the present invention.
Fig. 5 is the structural representation of bottle cap of the present invention
In figure:1st, bottle, 2, bottle cap, 3, base, 4, valve, 5, motor, 6, rotor, 7, stirring rod, 8, battery, 9, close Capping, 10, borded pile, 11, screw-casing hole, 12, through hole, 13, inactivator residual chamber, 14, ground glass, 15, pipeline, 16, valve Door socket.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.It is based on Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made Embodiment, belongs to the scope of protection of the invention.
Refer to shown in Fig. 1-5, a kind of Streptococcus suis culture inactivation integrated bottle, including bottle 1, the top of bottle 1 is set There is bottle cap 2, the bottom of bottle 1, which is provided with base 3, the bottle cap 2, is provided with inactivator residual chamber 13, the inactivator residual chamber 13 are connected by pipeline 15 with the bottle 1, and level is provided with valve 4 on the bottle cap 2, and the valve 4 blocks the pipeline 15, The top of inactivator residual chamber 13 is provided with closure 9, and the base 3 is provided with agitating device.
Level is provided with valve socket 16 on bottle cap 2, and the valve 4 is provided with through hole 12, the through hole 12 and the pipeline 15 is corresponding.
The left and right sides of pipeline 15 is provided with ground glass 14 on bottle cap 2, and the valve socket 16 is frosted mouthful, the valve 4 outer surfaces are provided with frosted, and the diameter of the through hole 12 is less than the diameter of the pipeline 15, sanding device can increase valve 4 with The contact area of valve socket 16, strengthens sealing effectiveness, and the diameter of through hole 12 is smaller, can effectively control the addition of inactivator Amount.
Splash bar device includes motor 5, rotor 6 and stirring rod 7, and the rotor 6 is arranged on the motor 5, the stirring Rod 7 is fixed on the rotor 6.
Rotor 6 is cylinder, and screw-casing hole 11 is provided with the middle of the rotor 6.
Stirring rod 7 is fold-line-shaped, and the bottom of stirring rod 7 is linear pattern borded pile 10, and the stirring rod 7 of fold-line-shaped can add Big stirring area, improves inactivation efficiency.
Screw-casing hole 11 matches with the borded pile 10.
Base 3 is provided with battery 8, and the battery 8 is connected with the motor 5, in the feelings supplied without extraneous power supply Under condition, battery 8 can power for motor 5.
Closure 9 is sheet rubber, when inactivating underdosage in inactivator residual chamber 13, is worn inactivator with sterile needle tubing Closure 9 is crossed to be injected into inactivator residual chamber 13.
Embodiment
After bacterium solution culture terminates, changeover valve 4, until through hole 12 and pipeline 15 on valve 4 are point-blank, now Inactivator in inactivator residual chamber 13 is instilled in bottle 1, is switched on power, rotor 6 rotates in the presence of motor 5, stirring rod 7 Inactivator is stirred with bacterium solution under the drive of rotor 6, inactivating efficacy is reached.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power Profit is required rather than described above is limited, it is intended that all in the implication and scope of the equivalency of claim by falling Change is included in the present invention.Any reference in claim should not be considered as to the claim involved by limitation.
Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each embodiment is only wrapped Containing an independent technical scheme, this narrating mode of specification is only that for clarity, those skilled in the art should Using specification as an entirety, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art It may be appreciated other embodiment.

Claims (9)

1. a kind of Streptococcus suis culture inactivation integrated bottle, including bottle (1), it is characterised in that:Bottle (1) top is provided with bottle Cover (2), bottle (1) bottom, which is provided with base (3), the bottle cap (2), is provided with inactivator residual chamber (13), the inactivator Residual chamber (13) is connected by pipeline (15) with the bottle (1), and level is provided with valve (4), the valve on the bottle cap (2) (4) pipeline (15) is blocked, inactivator residual chamber (13) top is provided with closure (9), and the base (3) is provided with and stirred Mix device.
2. Streptococcus suis culture inactivation integrated bottle according to claim 1, it is characterised in that:Level on the bottle cap (2) Provided with valve socket (16), the valve (4) is provided with through hole (12), and the through hole (12) is corresponding with the pipeline (15).
3. the Streptococcus suis culture inactivation integrated bottle according to claim 1 or claim 2, it is characterised in that:The bottle The left and right sides for building pipeline (15) on (2) is provided with ground glass (14), and the valve socket (16) is frosted mouthful, the valve (4) outer surface is provided with frosted, and the diameter of the through hole (12) is less than the diameter of the pipeline (15).
4. Streptococcus suis culture inactivation integrated bottle according to claim 1, it is characterised in that:The splash bar device includes electricity Machine (5), rotor (6) and stirring rod (7), the rotor (6) are arranged on the motor (5), and the stirring rod (7) is fixed on institute State on rotor (6).
5. Streptococcus suis culture inactivation integrated bottle according to claim 4, it is characterised in that:The rotor (6) is cylinder Screw-casing hole (11) are provided with the middle of shape, the rotor (6).
6. Streptococcus suis culture inactivation integrated bottle according to claim 4, it is characterised in that:The stirring rod (7) is folding Linear, stirring rod (7) bottom is linear pattern borded pile (10).
7. the Streptococcus suis culture inactivation integrated bottle according to claim 5 or claim 6, it is characterised in that:The spiral shell Rotation set hole (11) matches with the borded pile (10).
8. the Streptococcus suis culture inactivation integrated bottle according to claim 1 or claim 4, it is characterised in that:The bottom Seat (3) is provided with battery (8), and the battery (8) is connected with the motor (5).
9. the Streptococcus suis culture inactivation integrated bottle according to claim 1 or claim 4, it is characterised in that:It is described close It is sheet rubber to cover (9).
CN201710474995.9A 2017-06-21 2017-06-21 Streptococcus suis culture inactivation integrated bottle Pending CN107177486A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710474995.9A CN107177486A (en) 2017-06-21 2017-06-21 Streptococcus suis culture inactivation integrated bottle

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710474995.9A CN107177486A (en) 2017-06-21 2017-06-21 Streptococcus suis culture inactivation integrated bottle

Publications (1)

Publication Number Publication Date
CN107177486A true CN107177486A (en) 2017-09-19

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710474995.9A Pending CN107177486A (en) 2017-06-21 2017-06-21 Streptococcus suis culture inactivation integrated bottle

Country Status (1)

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CN (1) CN107177486A (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN204644370U (en) * 2015-05-22 2015-09-16 青岛易邦生物工程有限公司 Cell suspension culture device
CN205410651U (en) * 2015-12-08 2016-08-03 广州膳庭坊食品科技有限公司 Take handleless cup of valve
US20160265022A1 (en) * 2013-10-25 2016-09-15 Becton, Dickinson And Company Blood culture bottles with mechanisms for controlled release of substances into culture media

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160265022A1 (en) * 2013-10-25 2016-09-15 Becton, Dickinson And Company Blood culture bottles with mechanisms for controlled release of substances into culture media
CN204644370U (en) * 2015-05-22 2015-09-16 青岛易邦生物工程有限公司 Cell suspension culture device
CN205410651U (en) * 2015-12-08 2016-08-03 广州膳庭坊食品科技有限公司 Take handleless cup of valve

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
张立等: "《规模化猪场盈利模式》", 30 April 2016 *

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Application publication date: 20170919

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