CN112538427B - Intestinal lymphocyte culture apparatus - Google Patents
Intestinal lymphocyte culture apparatus Download PDFInfo
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- CN112538427B CN112538427B CN202011279751.3A CN202011279751A CN112538427B CN 112538427 B CN112538427 B CN 112538427B CN 202011279751 A CN202011279751 A CN 202011279751A CN 112538427 B CN112538427 B CN 112538427B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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Abstract
The invention relates to the technical field of cell culture equipment, in particular to an intestinal lymphocyte culture device; comprises a cylinder body, a covering mechanism and a sealing mechanism. The top of the cylinder body is provided with a bell mouth, and one side of the bell mouth is provided with a sealing hole communicated with the outside; the covering mechanism is detachably sleeved on the top of the bell mouth in a sealing manner, and a cavity is arranged between the top of the covering mechanism and the bell mouth; the sealing mechanism comprises a sealing plate, a pushing piece and a spring, the sealing plate is slidably inserted into the bell mouth, one end of the pushing piece is slidably and hermetically inserted into the sealing hole and fixedly connected with the sealing plate, the spring is sleeved on the pushing piece, two ends of the spring are fixedly connected with the barrel and the pushing piece respectively, and the barrel is sealed by the sealing plate in a natural state. The invention aims to solve the problem that intestinal lymphocytes cultured in a culture bottle are polluted because the culture bottle needs to be opened in the conventional culture device.
Description
Technical Field
The invention relates to the technical field of cell culture equipment, in particular to an intestinal lymphocyte culture device.
Background
The intestinal tract is the most complex mucosal immune organ of the body, is an important organ for identifying self and abnormal self, and is also a main door for pathogen invasion, so that the intestinal tract mucosal immune system forms the first immune barrier for resisting pathogen invasion.
The mucosal immune system includes lymphocyte, T cell, K cell immunity, etc. Among them, lymphocytes in the intestinal tract are the largest lymphocyte population in vivo. The main function of lymphocytes is cytolytic activity, a mechanism by which the host responds rapidly to pathogen invasion. I.e., lymphocytes, are the first line "soldiers" to fight external infection and monitor cellular variation in vivo.
At present, devices such as culture bottles and culture dishes are commonly used for culturing intestinal lymphocytes, and when workers culture the intestinal lymphocytes, the intestinal lymphocytes need to be shaken up, and medicines or liquid medicines such as colchicine and the like need to be added. However, when intestinal lymphocytes are cultured, at present, medicines or liquid medicines are added by opening a culture bottle, and a large amount of intestinal lymphocytes in a culture device are in contact with the outside, so that the intestinal lymphocytes cultured in the culture bottle are polluted.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides an intestinal lymphocyte culture device, which aims to solve the problem that intestinal lymphocytes cultured in a culture bottle are polluted because the culture bottle needs to be opened in the conventional culture device.
In order to achieve the purpose, the invention adopts the following technical scheme: an intestinal lymphocyte culture apparatus comprising:
the top of the cylinder is provided with a bell mouth, and one side of the bell mouth is provided with a sealing hole communicated with the outside;
the covering mechanism is detachably sleeved on the top of the bell mouth in a sealing manner, and a cavity is arranged between the top of the covering mechanism and the bell mouth; and
the sealing mechanism comprises a sealing plate, a pushing piece and a spring, wherein the sealing plate is slidably inserted into the horn mouth and can seal the narrow mouth of the horn mouth, one end part of the pushing piece is slidably inserted into the sealing hole in a sealing mode and is fixedly connected with the sealing plate, the spring is sleeved on the pushing piece, two ends of the spring are fixedly connected with the barrel body and the pushing piece respectively, and the sealing plate seals the barrel body in a natural state.
When the technical scheme is adopted, when intestinal lymphocytes are cultured in the cylinder body, the sealing plate seals the narrow opening at the bottom of the horn mouth, and the covering mechanism is hermetically sealed at the top of the cylinder body; when medicine or liquid medicine are added into the barrel, the cover is opened to close the mechanism, medicine or liquid medicine is added into the cavity, the cover is closed to close the mechanism, the pushing piece is pushed again, the spring stretches to accumulate force, the pushing piece pushes the sealing plate to move upwards, and then the sealing plate is separated from the narrow opening of the horn mouth, so that the medicine or the liquid medicine falling into the horn mouth enters the barrel, and after the spring is reset, the sealing plate seals the barrel. Thereby avoiding the liquid medicine in the cylinder body from contacting with the outside, and reducing the pollution of intestinal tract lymphocytes cultured in the culture bottle.
Preferably, the mechanism that closes includes casing and medical rubber stopper, go up casing seal install in the flaring department of horn mouth, go up the top of casing with form between the horn mouth the cavity, the top of going up the casing is equipped with the opening, medical rubber stopper fixed mounting is on last casing, and is located the top of cavity, and can seal the opening.
Pass the opening through the syringe needle and pierce medical rubber stopper, and then be convenient for add liquid medicine or medicine in the cavity, and then avoid opening the closure mechanism, and then can further avoid intestinal lymphocyte to receive the pollution.
Preferably, the pushing member comprises a pushing rod and a supporting rod, the pushing rod is slidably and hermetically mounted in the sealing hole, the axial direction of the pushing rod is parallel to the height direction of the barrel, two ends of the supporting rod are respectively fixedly connected with the sealing plate and the top of the pushing rod, and the spring is sleeved on the pushing rod.
Promote the catch bar for the catch bar rises, and the spring holds power, makes the bracing piece drive closing plate upward movement, and then breaks away from the slot of horn mouth, loosens the spring, and the spring resets the back, makes the closing plate seal up the barrel.
Preferably, the device further comprises a rotating mechanism, the rotating mechanism is rotatably sleeved on the barrel, the top of the rotating mechanism is located at the bottom of the push rod, and the rotating mechanism can push the push rod to move axially.
The arrangement of the rotating mechanism can better push the push rod.
Preferably, the rotating mechanism comprises a sleeve barrel, a ball nut and a threaded barrel, the sleeve barrel is rotatably sleeved at the bottom of the barrel, a gap exists between the inner wall of the sleeve barrel and the barrel, the ball nut is fixedly connected with the inner wall of the sleeve barrel, the threaded barrel is axially slidably sleeved on the barrel and is slidably inserted into the gap, the top of the threaded barrel is located at the bottom of the push rod, the threaded barrel can push the push rod to move axially, and the threaded barrel is in threaded connection with the ball nut.
The sleeve barrel is rotated to drive the ball nut to rotate, so that the threaded barrel moves upwards or downwards, and the push rod can be better pushed through the threaded barrel.
Preferably, the lymphocyte culture device further comprises a stirring mechanism, wherein one end part of the stirring mechanism is rotatably arranged at the bottom of the cylinder body, and the stirring mechanism can stir the lymphocytes in the cylinder body.
Through the setting of rabbling mechanism, be convenient for stir the intestinal lymphocyte in the barrel.
Preferably, the stirring mechanism comprises a rotating disc, a stirring column and a rotating column, the rotating disc is rotatably and hermetically mounted at the bottom of the cylinder body, the stirring column is fixedly mounted at the top of the rotating disc, and the rotating column hermetically penetrates through the bottom of the cylinder body and is fixedly connected with the sleeve barrel.
When rotating the cover bucket, the cover bucket can also drive and rotate the post, rotates the post and then drives the rolling disc and rotates to stir lymphocyte through rolling disc and puddler.
The beneficial effect that this scheme produced is:
1. through the horn mouth of barrel, the setting of closing mechanism, closing plate, impeller and spring, open the mechanism that closes of lid, add medicine or liquid medicine in to the cavity, the impeller drives the closing plate upward movement, and then breaks away from the slot of horn mouth for fall into the medicine or the liquid medicine in the horn mouth and enter into the barrel. Thereby avoiding the liquid medicine in the cylinder body from contacting with the outside, and reducing the pollution of intestinal lymphocytes cultured in the culture bottle.
2. Through the setting of cover bucket, ball nut, screw thread bucket, rolling disc, stirring post and rotation post, rotate the cover bucket, the cover bucket not only can drive ball nut and rotate for screw thread bucket promotes catch bar upward movement, and then is convenient for realize in liquid medicine or the medicine adds the barrel, the cover bucket can drive the rotation post moreover and rotate, thereby realizes shaking evenly lymphocyte and the liquid medicine or the medicine of adding.
Drawings
In order to more clearly illustrate the embodiments of the present invention, the drawings, which are required to be used in the embodiments, will be briefly described below. The elements or parts are not necessarily drawn to scale in all figures.
FIG. 1 is a front view of an intestinal lymphocyte culture apparatus according to the present invention;
FIG. 2 isbase:Sub>A schematic view in the direction A-A in FIG. 1;
FIG. 3 is a schematic view in the direction B-B of FIG. 1;
FIG. 4 is a top view of an intestinal lymphocyte culture apparatus according to the present invention.
Reference numerals:
1-cylinder, 11-bell mouth;
2-a covering mechanism, 21-an upper shell, 22-a medical rubber plug and 23-an opening;
3-sealing mechanism, 31-sealing plate, 32-pushing piece, 321-pushing rod, 322-supporting rod and 33-spring;
4-rotating mechanism, 41-barrel set, 42-ball nut, 43-screw barrel;
5-stirring mechanism, 51-rotating disk, 52-stirring column and 53-rotating column.
Detailed Description
Embodiments of the present invention will be described in detail below with reference to the accompanying drawings. The following examples are only for illustrating the technical solutions of the present invention more clearly, and therefore are only used as examples, and the protection scope of the present invention is not limited thereby.
Referring to fig. 1-4, an intestinal lymphocyte culture apparatus includes a cylinder 1, a covering mechanism 2 and a sealing mechanism 3.
Specifically, as shown in fig. 1, the top of the cylinder 1 is in the shape of a bell mouth 11, and one side of the bell mouth 11 is provided with a sealing hole communicated with the outside. In this embodiment, the left and right sides of the bottom of the bell mouth 11 are both provided with sealing holes. So that the sealing plate 31 can better seal the bell mouth 11.
Specifically, the covering mechanism 2 is sealed and installed at the top of the bell mouth 11 through bolt fixing, and a cavity is arranged between the top of the covering mechanism 2 and the bell mouth 11. Wherein, closing mechanism 2 includes upper housing 21 and medical rubber stopper 22, and upper housing 21 seal installation forms the cavity in the top of the flaring of horn mouth 11 between upper housing 21's top and the horn mouth 11, and upper housing 21's top is equipped with opening 23, and medical rubber stopper 22 fixed mounting is on upper housing 21 to be located the top of cavity, and can seal opening 23. The needle head penetrates through the opening 23 and pierces the medical rubber plug 22, so that liquid medicine or powder solid medicine and the like can be conveniently added into the chamber, the covering mechanism 2 is prevented from being opened, the intestinal lymphocyte is prevented from contacting with the outside air, and the pollution is reduced.
Specifically, as shown in fig. 1 and fig. 2, the sealing mechanism 3 includes a sealing plate 31, a pushing member 32 and a spring 33, the sealing plate 31 is inserted into the bell mouth 11 in a vertically slidable manner and can seal the narrow mouth of the bell mouth 11, one end of the pushing member 32 is inserted into the sealing hole in a slidable and sealed manner and is fixedly connected with the sealing plate 31, the spring 33 is sleeved on the pushing member 32, two ends of the spring 33 are fixedly connected with the cylinder 1 and the pushing member 32, respectively, and in a natural state, the sealing plate 31 seals the cylinder 1.
In this embodiment, the pushing element 32 includes a pushing rod 321 and a supporting rod 322, the pushing rod 321 is slidably and hermetically mounted in the sealing hole up and down, an axial direction of the pushing rod 321 is parallel to a height direction of the cylinder 1, two ends of the supporting rod 322 are respectively fixedly connected to the sealing plate 31 and the top of the pushing rod 321, and the spring 33 is sleeved on the pushing rod 321. Wherein, still can set up tubbiness board in the cavity, the cover plate of the bucket back-off is in the outside of impeller 32 and spring 33 for the bung hole and the sealing hole sealing connection of cover plate of the bucket not only are used for protecting spring 33, and the cover plate of the bucket further seals the sealing hole. Through pushing the push rod 321, the push rod 321 rises, the spring 33 accumulates force, the support rod 322 drives the sealing plate 31 to move upwards, and then the sealing plate 31 is separated from the narrow opening of the bell mouth 11, the spring 33 is loosened, and the sealing plate 31 seals the cylinder body 1 after the spring 33 is reset.
In order to facilitate the use of the intestinal lymphocyte culture apparatus, in this embodiment, as shown in fig. 1 and 3, a rotation mechanism 4 and a stirring mechanism 5 are further included.
Wherein, the rotating mechanism 4 is rotatably sleeved on the barrel 1, the top of the rotating mechanism 4 is positioned at the bottom of the push rod 321, and the rotating mechanism 4 can push the push rod 321 to move axially. The rotating mechanism 4 is arranged to push the push rod 321 better. In this embodiment, the rotating mechanism 4 includes a sleeve barrel 41, a ball nut 42, and a threaded barrel 43. The sleeve barrel 41 is rotatably sleeved at the bottom of the barrel body 1, a gap exists between the inner wall of the sleeve barrel 41 and the barrel body 1, the ball nut 42 is fixedly connected with the inner wall of the sleeve barrel 41, the threaded barrel 43 is axially slidably sleeved on the barrel body 1 and can be slidably inserted into the gap, the top of the threaded barrel 43 is positioned at the bottom of the push rod 321, the threaded barrel 43 can push the push rod 321 to axially move, and the threaded barrel 43 is in threaded connection with the ball nut 42. The sleeve barrel 41 is rotated, the sleeve barrel 41 drives the ball nut 42 to rotate, and then the screw barrel 43 moves up or down, so that the push rod 321 can be better pushed by the screw barrel 43.
Wherein, one end of the stirring mechanism 5 is rotatably installed at the bottom of the cylinder 1, and the stirring mechanism 5 can stir the lymphocyte in the cylinder 1. In this embodiment, the stirring mechanism includes a rotating disc 51, a stirring column 52 and a rotating column 53, the rotating disc 51 is rotatably and hermetically mounted at the bottom of the cylinder body 1, the stirring column 52 is fixedly mounted at the top of the rotating disc 51, and the rotating column 53 is hermetically passed through the bottom of the cylinder body 1 and is fixedly connected with the sleeve barrel 41. When the sleeve barrel 41 is rotated, the sleeve barrel 41 can also drive the rotating column 53 to rotate, and the rotating column 53 further drives the rotating disc 51 to rotate, so that the lymphocytes are stirred through the rotating disc 51 and the stirring rod. Meanwhile, after the rotating disc 51 is connected through the rotating column 53, the axial movement of the sleeve barrel 41 is limited, and the threaded barrel 43 can be driven to move up and down more stably during rotation.
When the intestinal lymphocyte culture device is used, when intestinal lymphocytes are cultured in the cylinder body 1, the sealing plate 31 seals the narrow opening at the bottom of the bell mouth 11, and then the intestinal lymphocytes in the cylinder body 1 are cultured in a sealing manner. When medicine or liquid medicine needs to be added into the barrel body 1, the medical rubber plug 22 is penetrated through the opening 23 by an external needle, so that the liquid medicine or the medicine can be conveniently added into the chamber, and the intestinal lymphocytes are reduced from being exposed to the outside.
Meanwhile, the sleeve barrel 41 is rotated, the sleeve barrel 41 drives the ball nut 42 to rotate, the ball nut 42 drives the threaded barrel 43 to move upwards, the threaded barrel 43 pushes the push rod 321 to move upwards, the spring 33 is lengthened to accumulate force, the support rod 322 drives the sealing plate 31 to move upwards, the sealing plate 31 is separated from the narrow opening of the bell-mouth 11, the cavity is communicated with the barrel body 1, and the medicine or the liquid medicine falling into the cavity falls into the barrel body 1; after the liquid medicine or the medicine is added, the sleeve barrel 41 is rotated reversely, the spring 33 is reset, and the sealing plate 31 is driven to seal the barrel body 1; thereby reducing the contact time of intestinal tract lymphocytes in the cylinder body 1 with the outside and further avoiding the pollution of the intestinal tract lymphocytes.
And, when rotating cover bucket 41, cover bucket 41 can also drive and rotate post 53 and rotate, rotates post 53 and then drives rolling disc 51 and rotate to stir through rolling disc 51 and puddler intestinal lymphocyte in to barrel 1 and liquid medicine or medicine and shake evenly, thereby make the cultivation of intestinal lymphocyte more abundant.
Above technical scheme closes mechanism 2, closing plate 31, impeller 32 and spring 33's setting through horn mouth 11, the lid of barrel 1, opens closing mechanism 2, adds medicine or liquid medicine in to the cavity, and impeller 32 drives closing plate 31 upward movement, and then breaks away from the slot of horn mouth 11 for medicine or liquid medicine that fall into in horn mouth 11 enter into in the barrel 1. Thereby avoiding the liquid medicine in the cylinder body 1 from contacting with the outside, and reducing the pollution of intestinal lymphocytes cultured in the culture bottle.
Through the setting of cover bucket 41, ball nut 42, screw thread bucket 43, rolling disc 51, stirring post 52 and rotation post 53, rotate cover bucket 41, cover bucket 41 not only can drive ball nut 42 and rotate for screw thread bucket 43 promotes catch bar 321 upward movement, and then be convenient for realize in liquid medicine or the medicine adds barrel 1, and cover bucket 41 can drive rotation post 53 and rotate in addition, thereby the realization stirs the mixture to lymphocyte and liquid medicine or the medicine of adding.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention, and they should be construed as being included in the following claims and description.
Claims (2)
1. An intestinal lymphocyte culture device, comprising:
the top of the cylinder is provided with a bell mouth, and one side of the bell mouth is provided with a sealing hole communicated with the outside;
the covering mechanism is detachably sleeved on the top of the bell mouth in a sealing manner, and a cavity is arranged between the top of the covering mechanism and the bell mouth;
the sealing mechanism comprises a sealing plate, a pushing piece and a spring, the sealing plate is slidably inserted into the horn mouth and can seal the narrow mouth of the horn mouth, one end part of the pushing piece is slidably inserted into the sealing hole in a sealing manner and is fixedly connected with the sealing plate, the spring is sleeved on the pushing piece, two ends of the spring are fixedly connected with the barrel body and the pushing piece respectively, and the sealing plate seals the barrel body in a natural state; the pushing piece comprises a pushing rod and a supporting rod, the pushing rod is slidably and hermetically arranged in the sealing hole, the axial direction of the pushing rod is parallel to the height direction of the barrel, two ends of the supporting rod are respectively fixedly connected with the sealing plate and the top of the pushing rod, and the spring is sleeved on the pushing rod;
the rotating mechanism is rotatably sleeved on the barrel, the top of the rotating mechanism is positioned at the bottom of the push rod, and the rotating mechanism can push the push rod to move axially; the rotating mechanism comprises a sleeve barrel, a ball nut and a threaded barrel, the sleeve barrel is rotatably sleeved at the bottom of the barrel, a gap is formed between the inner wall of the sleeve barrel and the barrel, the ball nut is fixedly connected with the inner wall of the sleeve barrel, the threaded barrel is axially slidably sleeved on the barrel and is slidably inserted into the gap, the top of the threaded barrel is positioned at the bottom of the push rod, the threaded barrel can push the push rod to axially move, and the threaded barrel is in threaded connection with the ball nut; and
one end of the stirring mechanism is rotatably arranged at the bottom of the cylinder body, and the stirring mechanism can stir the lymphocytes in the cylinder body; the stirring mechanism comprises a rotating disc, a stirring column and a rotating column, the rotating disc is rotatably and hermetically arranged at the bottom of the barrel body, the stirring column is fixedly arranged at the top of the rotating disc, and the rotating column penetrates through the bottom of the barrel body in a sealing manner and is fixedly connected with the sleeve barrel.
2. The intestinal tract lymphocyte culture device according to claim 1, wherein the covering mechanism comprises an upper shell and a medical rubber plug, the upper shell is hermetically installed at the flaring part of the bell mouth, the chamber is formed between the top of the upper shell and the bell mouth, the top of the upper shell is provided with an opening, and the medical rubber plug is fixedly installed on the upper shell, is positioned at the top of the chamber and can seal the opening.
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| CN202011279751.3A CN112538427B (en) | 2020-11-16 | 2020-11-16 | Intestinal lymphocyte culture apparatus |
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| CN202011279751.3A CN112538427B (en) | 2020-11-16 | 2020-11-16 | Intestinal lymphocyte culture apparatus |
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| CN112538427B true CN112538427B (en) | 2023-04-07 |
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| CN205954029U (en) * | 2016-08-24 | 2017-02-15 | 北京华诺奥美基因生物科技有限公司 | Lymphocyte blake bottle |
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