CN114703053A - Device for culturing algae at constant temperature and experimental method - Google Patents
Device for culturing algae at constant temperature and experimental method Download PDFInfo
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
Abstract
The invention discloses a device for culturing algae at constant temperature and an experimental method, comprising a water pool, a transparent culture cylinder, water inlet and outlet equipment, a heating, illuminating and gas supplying structure and other structures, and further comprising the experimental method from the first step to the fifth step; the culture device designed by the invention adopts a design concept of water bath temperature control, and has the advantages of controllable temperature, stable heating and uniform heating of algae; the transparent culture cylinder and the mounting structure thereof can reduce the entrance of air pollution particles; the water used by the insulating layer can be recycled by the water in the pool; the design of the invention adopts a main control box to control the illumination intensity, and adopts a timing device to control a lamp tube to control the illumination time to reach the optimal growth condition; the design concept of water bath temperature control is used, the device has the advantages of controllable temperature, stable heating and uniform heating of algae, and redundant water is discharged through a drainage pipeline above the acrylic plate column and enters the water pool again to realize water recycling.
Description
Technical Field
The invention relates to the technical field of algae culture, in particular to a device for culturing algae at constant temperature and an experimental method.
Background
The microalgae is a unicellular organism with high oil content, particularly the chlorella, the oil content can reach 20% of the dry weight of cells, various nutritional ingredients can be used for preparing biodiesel, the biodiesel can be taken as a nutritional supplement, the chlorella has the effects of resisting aging, beautifying and the like, scenedesmus contains rich protein and vitamins, and the scenedesmus is a good feed for fish, and high yield can be obtained if the fish is cultured in an organic sewage oxidation pond. The mass-propagated algae can also be used as feed for poultry. Meanwhile, the microalgae has wide application prospects in the aspects of feeds, foods, medicines and cosmetics. The basis of these applications is the large-scale cultivation of microalgae.
During the cultivation of algae, the growth and propagation speed of algae has certain fluctuation, and the growth mode can be divided into five periods (delay period, exponential growth period, relative growth decline period, stationary period and death period).
Currently, although various methods of culturing algae are required for the purpose of culturing algae, the methods can be classified into closed culture and open culture. The purpose of closed culture is to prevent foreign miscellaneous algae, fungi and other organisms from being mixed into the culture. The culture solution is sealed in a transparent container completely isolated from the outside, and thus the devices for ventilating, stirring, transporting the culture solution, adjusting water temperature, sampling, and the like are also isolated from the outside.The culture container is tubular and pond-shaped, and is made of organic glass or transparent polyethylene plastic into horizontal pipeline, and is erected on the ground vertically or obliquely and exposed to sunlight or artificial light. The culture method has the advantages of high required cost, large material consumption and high required energy. Open cultivation is the cultivation of algae in open containers (e.g., cement ponds, pipes, wooden basins, etc.). Open cultures can be classified into several types: (1) open cycle culture: it is characterized in that the culture solution continuously circularly flows by means of a circulating water pump; (2) open non-circulating culture: it features that the culture liquid does not flow circularly and CO is periodically introduced via small tube2And air into the culture; (3) semi-open circulation culture: semi-open culture means that some parts of culture vessels, tanks, and tanks are closed or covered with plastic cloth, although the culture vessels, tanks, and tanks are still open. This culture method uses a pipe and uses power to flow and introduce the culture solution into the air containing carbon dioxide, but the method is complicated in equipment. Biological contamination, such as bacterial growth, can easily occur in the open culture.
At present, in the device for culturing algae, the mode of culturing by using an incubator is mostly adopted, the incubator is of a sealed opaque structure, the incubator is not suitable for directly observing the growth condition of the algae, an air pump needs to be periodically replaced to keep the internal carbon source of the algae sufficient, meanwhile, the temperature in the incubator can not be directly observed, the temperature condition is difficult to control, the algae culture failure is caused, and therefore the culture device with controllable temperature, simple equipment and less energy consumption is needed.
Disclosure of Invention
The invention aims to provide a device for culturing algae at constant temperature and an experimental method, which are used for providing a stable culture method for algae so as to solve the problems in the prior art in the background technology.
In order to achieve the purpose, the invention adopts the following technical scheme:
a device for culturing algae at constant temperature comprises a water pool and a transparent culture cylinder, wherein a heating rod and a temperature sensor are arranged inside the water pool, a water pumping pump is arranged outside the water pool, the output end of the water pumping pump is fixedly communicated with a water inlet pipeline, and the water inlet pipeline is fixedly communicated with the transparent culture cylinder;
the transparent culture cylinder is of a double-layer cylindrical structure and is provided with two transparent culture cylinders, a lamp tube and an aeration head are arranged in each transparent culture cylinder, and an air inlet pipeline, a drainage pipeline and a water outlet are arranged on the outer side of each transparent culture cylinder; the pond is inside to be provided with temperature sensor and heating rod and the pond outside is provided with the water pump, the water pump passes through the inlet channel and imports water in the transparent culture section of thick bamboo intermediate layer.
Preferably, the temperature of the water in the water tank is monitored by a temperature sensor, the heating time is controlled by a main control box, a water pumping pump is arranged outside the water tank, the flowing circulation of the water in the water tank and the water in an interlayer of a transparent culture cylinder is realized through a water inlet pipeline, the transparent culture cylinder can culture at least 6.5L of algae, and at least 7L of water can be stored in the interlayer outside the transparent culture cylinder.
Preferably, temperature sensor's quantity is seven, and one of them is installed at the inside monitoring temperature in pond, and six evenly distributed are left now on two transparent culture tubes for monitoring the algae temperature and show the temperature by the master control case respectively, transparent culture tube includes two ya keli board posts, and every ya keli board post outside sets up three temperature sensor.
Preferably, the algae cultivation device further comprises an air pump, one end of the air pump is connected with the flowmeter through a hose, the other end of the air pump is connected with the aeration head through an air inlet pipeline so as to control the aeration amount of algae in the transparent culture cylinder, and the lamp tube can control the illumination time of the algae through a timing device.
Preferably, the number of the water outlets is four, and the water outlets are uniformly distributed on the transparent culture cylinder.
Preferably, the lamp tube is arranged in the transparent culture cylinder, one end of the lamp tube is connected with the timing device, while the other end of the lamp tube is connected with the main control box, and the main control box is used for controlling the brightness of the lamp tube.
Preferably, at least six groups of stop valves are distributed on the water inlet pipeline and the water discharge pipeline.
The invention also provides an experimental method of the device for culturing algae at constant temperature, which comprises the following steps:
the method comprises the following steps: turning on a main power supply, filling tap water into the water tank, turning on a main control box, displaying the water temperature measured by a temperature sensor by the main control box, turning on a water pump arranged outside the water tank, conveying the water heated in the water tank into an interlayer of a transparent culture cylinder through a water inlet pipeline, and discharging the water into the water tank through a water discharge pipeline above the transparent culture cylinder to realize the constant-temperature culture of algae;
step two: the stop valve of the left water pumping pipeline is closed, the stop valve of the water pumping pipeline on the right transparent culture cylinder is opened, and water flow passes through the water bath heat preservation layer of the transparent culture cylinder, so that the left algae culture device and the right algae culture device can be used independently;
step three: algae to be cultured is added into the transparent culture cylinder, the temperature of the algae measured by the temperature sensor is displayed by the main control box, and when the temperature of the algae reaches a certain value, the operation of the heating rod is controlled, and the main control box can independently control the operation of the left culture device and the right culture device;
step four: opening an air pump, connecting one end of the air pump with an aeration head through an air inlet pipeline, connecting one end of the air pump with a flowmeter through a hose, and adjusting the flowmeter to control the amount of carbon dioxide required by the growth of algae;
step five: and opening a lamp tube arranged in the transparent culture cylinder, wherein one end of the lamp tube is connected with a timing device, the other end of the lamp tube is connected with the main control box, and the timing device is opened and is used for controlling the lamp tube to be bright for reaching the optimal growth condition of algae.
The invention has the technical effects and advantages that: compared with the prior art, the device for culturing algae at constant temperature and the experimental method provided by the invention have the following advantages:
compared with the prior art, the invention provides the indoor constant-temperature algae cultivation device, the cultivation device designed by the invention adopts the design concept of water bath temperature control, and has the advantages of controllable temperature, stable heating and uniform algae heating; the transparent culture cylinder and the mounting structure thereof can reduce the entrance of air pollution particles; the water used by the insulating layer can be recycled by the water in the pool; the invention adopts the main control box to control the illumination intensity, and adopts the timing device to control the lighting time of the lamp tube, thereby controlling the illumination time of algae and achieving the optimal growth condition;
when the invention is used, the left transparent culture cylinder and the right transparent culture cylinder can be independently used, and the flow of water in the interlayer is controlled by controlling the stop valve on the water pumping pipeline; the heating water in the water tank is input into the interlayer through the water pump, the design concept of water bath temperature control is used, the advantages of controllable temperature, stable heating and uniform heating of algae are achieved, and redundant water is discharged through the drainage pipeline above the acrylic plate column and enters the water tank again to achieve water recycling.
Additional features and advantages of the invention will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. The objectives and other advantages of the invention will be realized and attained by the structure particularly pointed out in the written description and drawings.
Drawings
FIG. 1 is a schematic overall front cut-away configuration of the present invention;
FIG. 2 is a schematic view of the overall top cut-away construction of the present invention;
FIG. 3 is a schematic overall side view cutaway construction of the present invention;
FIG. 4 is a graph showing the temperature results of Scenedesmus cultivation according to the present invention;
FIG. 5 is a graph showing the results of absorbance of Scenedesmus culture according to the present invention;
FIG. 6 is a graph showing the pH results of Scenedesmus cultivation according to the present invention;
FIG. 7 is a graph showing temperature results of culturing chlorella of the present invention;
FIG. 8 is a graph showing the results of absorbance of cultured chlorella of the present invention;
FIG. 9 is a graph showing the pH results of chlorella culture according to the present invention;
in the figure: 1. a pool; 2. a heating rod; 3. a water pump; 4. a flow meter; 5. a transparent culture cylinder; 6. a water outlet; 7. a timing device; 8. a master control box; 9. an air pump; 10. a water inlet pipe; 11. a hose; 12. an aeration head; 13. a temperature sensor; 14. an air intake duct; 15. a lamp tube; 16. a water discharge pipeline; 17. a stop valve; 18. a glass column; 19. and (5) sealing an acrylic plate.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. The specific embodiments described herein are merely illustrative of the invention and do not delimit the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides example 1 as shown in figures 1-3:
a device for culturing algae at constant temperature comprises a water pool 1 and a transparent culture cylinder 5, wherein a heating rod 2 and a temperature sensor 13 are installed inside the water pool 1, a water pumping pump 3 is installed outside the water pool 1, the output end of the water pumping pump 3 is fixedly communicated with a water inlet pipeline 10, and the water inlet pipeline 10 is fixedly communicated with the transparent culture cylinder 5;
the transparent culture cylinder 5 is of a double-layer cylindrical structure and is provided with two parts, a lamp tube 15 and an aeration head 12 are arranged in the transparent culture cylinder 5, and an air inlet pipeline 14, a drainage pipeline 16 and a water outlet 6 are arranged on the outer side of the transparent culture cylinder 5; the inside temperature sensor 13 and the heating rod 2 that are provided with of pond 1 and the 1 outside in pond are provided with water pump 3, water pump 3 passes through inlet channel 10 and imports water in the transparent culture section of thick bamboo 5 intermediate layer.
The inside temperature of pond 1 is monitored by temperature sensor 13 and is controlled the heating time by master control case 8, and the outside sets up water pump 3 and realizes the water flow circulation in the water in pond 1 and the 5 intermediate layers of transparent culture cylinder through inlet channel 10, and is single transparent culture cylinder 5 can cultivate at least 6.5L algae, and the external intermediate layer can store 7L at least water.
The quantity of temperature sensor 13 is seven, and one of them is installed in 1 inside monitoring temperature in pond, and six evenly distributed remain now on two transparent culture tubes 5 for monitoring algae temperature and show the temperature by master control case 8 respectively, transparent culture tube 5 includes two ya keli board posts, and every ya keli board post outside sets up three temperature sensor 13.
The algae cultivation device further comprises an air pump 9, one end of the air pump 9 is connected with the flowmeter 4 through a hose 11, the other end of the air pump 9 is connected with an aeration head 12 through an air inlet pipeline 14 so as to control the aeration amount of algae in the transparent culture cylinder 5, and the lighting tube 15 can control the illumination time of the algae through the timing device 7.
The number of the water outlets 6 is four, and the water outlets are uniformly distributed on the transparent culture cylinder 5; the lamp tube 15 is arranged in the transparent culture cylinder 5, one end of the lamp tube is connected with the timing device 7, the other end of the lamp tube is connected with the main control box 8, and the main control box 8 is used for controlling the brightness of the lamp tube 15; at least six groups of stop valves 17 are distributed on the water inlet pipeline 10 and the water discharge pipeline 16;
specifically, eight groups of glass columns 18 are fixedly mounted at the bottom of the transparent culture cylinder 5, an acrylic plate sealing plate 19 is arranged at the top of the acrylic plate column of the transparent culture cylinder 5, and the glass columns 18 are uniformly distributed below the transparent culture cylinder 5 for fixed mounting; the acrylic plate sealing plate 19 covers the top of the transparent culture cylinder 5, the transparent culture cylinder 5 is isolated from the external space, and air pollution particles can be reduced by the acrylic plate sealing plate 19.
The invention also provides an experimental method of the device for culturing algae at constant temperature, which comprises the following steps:
the method comprises the following steps: a main power supply is turned on, tap water is filled into the water pool 1, a main control box 8 is turned on, the main control box 8 displays the water temperature measured by a temperature sensor 13, a water pumping pump 3 arranged outside the water pool 1 is turned on, the water heated in the water pool 1 is conveyed into an interlayer of a transparent culture cylinder 5 through a water inlet pipeline 10, and the water is discharged into the water pool 1 through a water discharge pipeline 16 above the transparent culture cylinder 5, so that the algae is cultured at constant temperature;
step two: the stop valve 17 of the left water pumping pipeline is closed, the stop valve 17 of the water pumping pipeline on the right transparent culture cylinder 5 is opened, water flow passes through the water bath heat preservation layer of the transparent culture cylinder 5, and the left algae culture device and the right algae culture device can be used independently;
step three: algae to be cultured is added into the transparent culture cylinder 5, the temperature of the algae measured by the temperature sensor 13 is displayed by the main control box 8, when the temperature of the algae reaches a certain value, the operation of the heating rod 2 is controlled, and the main control box 8 can independently control the operation of the left culture device and the right culture device;
step four: opening the air pump 9, connecting one end of the air pump with the aeration head 12 through the air inlet pipeline 14, connecting one end of the air pump with the flowmeter 4 through the hose 11, and adjusting the flowmeter 4 to control the amount of carbon dioxide required by the growth of algae;
step five: and opening a lamp tube 15 arranged in the transparent culture cylinder 5, wherein one end of the lamp tube 15 is connected with a timing device 7, the other end of the lamp tube 15 is connected with a main control box 8, and the timing device 7 is opened and is used for controlling the brightness time of the lamp tube 15 so as to achieve the optimal growth condition of algae.
Referring to fig. 4 to 9, an experimental method of an apparatus for culturing algae at a constant temperature according to example 2 of the present invention, based on example 1, includes the following steps:
the method comprises the following steps: weighing 1.7g BG-11 culture medium, dissolving in 1L pure water, sterilizing at 121 deg.C for 25min to obtain algae culture solution;
step two: pouring tap water into the water pool, submerging the temperature sensor, simultaneously starting the main control box, controlling the water temperature of the water pool to be 20-30 ℃, opening a valve on a water pumping pipeline, starting a power supply of a water pumping pump, inputting the water of the water pool into an interlayer of the transparent culture cylinder 5, simultaneously starting a lamp tube switch, and controlling the illumination time to be 12 hours and the illumination intensity to be 5000 plus 10000Lux by a timing device;
step three: closing a stop valve below the acrylic plate column, closing a water outlet, pouring 5L of scenedesmus into the column, and pouring the scenedesmus into the column according to the proportion of 1: 5, pouring 1L of culture solution, simultaneously turning on a power supply of an air pump, and controlling the aeration degree in the scenedesmus to be 15L/H by controlling a flow meter;
step four: at the same time every day, respectively recording the water temperature and the algae temperature of a water tank, opening a lower-layer water outlet, taking 15ml of scenedesmus, placing the scenedesmus in a beaker, measuring and recording the pH value and the absorbance, and after recording for one week, continuing to perform the following steps according to the ratio of 1: 5 adding culture solution, the result is shown in fig. 4, 5 and 6, the algae temperature can be controlled at 25-30 deg.C and kept relatively stable, and Scenedesmus is in the growth phase first and then in the stable phase.
Step five: as can be seen from FIGS. 7, 8 and 9, chlorella can be cultured and kept stable under the device.
In conclusion, compared with the prior art, the indoor constant-temperature algae cultivation device provided by the invention adopts a water bath temperature control design concept, and has the advantages of controllable temperature, stable heating and uniform algae heating; the transparent culture cylinder and the mounting structure thereof can reduce the entrance of air pollution particles; the water used by the insulating layer can be recycled by the water in the pool; the invention adopts the main control box to control the illumination intensity and adopts the timing device to control the lighting time of the lamp tube, thereby controlling the illumination time of the algae and achieving the optimal growth condition; when the device is used, the left transparent culture cylinder and the right transparent culture cylinder can be independently used, and the flow of water in the interlayer is controlled by controlling the stop valve on the water pumping pipeline; the heating water in the water tank is input into the interlayer through the water pump, the design concept of water bath temperature control is used, the advantages of controllable temperature, stable heating and uniform heating of algae are achieved, and redundant water is discharged through the drainage pipeline above the acrylic plate column and enters the water tank again to achieve water recycling.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.
Claims (8)
1. The device for culturing algae at constant temperature is characterized by comprising a water pool (1) and a transparent culture cylinder (5), wherein a heating rod (2) and a temperature sensor (13) are installed inside the water pool (1), a water pumping pump (3) is installed outside the water pool (1), the output end of the water pumping pump (3) is fixedly communicated with a water inlet pipeline (10), and the water inlet pipeline (10) is fixedly communicated with the transparent culture cylinder (5);
the transparent culture cylinder (5) is of a double-layer cylindrical structure and is provided with two tubes, a lamp tube (15) and an aeration head (12) are arranged in the transparent culture cylinder (5), and an air inlet pipeline (14), a drainage pipeline (16) and a water outlet (6) are arranged on the outer side of the transparent culture cylinder (5); the utility model discloses a transparent culture cylinder, including pond (1), pond (1) inside be provided with temperature sensor (13) and heating rod (2) and pond (1) outside is provided with water-pumping pump (3), water-pumping pump (3) are inputed water in transparent culture cylinder (5) intermediate layer through inlet channel (10).
2. The apparatus for cultivating algae at constant temperature according to claim 1, wherein: the inside temperature of pond (1) is monitored by temperature sensor (13) and by master control case (8) control heating time, and the outside sets up water pump (3) and realizes the water flow circulation in the water in pond (1) and transparent culture cylinder (5) intermediate layer through inlet channel (10), and is single transparent culture cylinder (5) can cultivate at least 6.5L alga, and the external intermediate layer can store at least 7L water.
3. The apparatus for cultivating algae at constant temperature according to claim 2, wherein: the quantity of temperature sensor (13) is seven, and one of them is installed in pond (1) inside monitoring temperature, and six evenly distributed remain now on two transparent culture tubes (5) for monitor algae temperature and show the temperature by master control case (8) respectively, transparent culture tube (5) include two ya keli board posts, and every ya keli board post outside sets up three temperature sensor (13).
4. The apparatus for cultivating algae at constant temperature according to claim 3, wherein: the algae cultivation device is characterized by further comprising an air pump (9), wherein one end of the air pump (9) is connected with the flowmeter (4) through a hose (11), the other end of the air pump is connected with an aeration head (12) through an air inlet pipeline (14) so as to control the aeration quantity of algae in the transparent cultivation cylinder (5), and the lighting time of the algae can be controlled through the timing device (7) through the lamp tube (15).
5. The apparatus for cultivating algae at constant temperature according to claim 4, wherein: the number of the water outlets (6) is four, and the water outlets are uniformly distributed on the transparent culture cylinder (5).
6. An apparatus for cultivating algae at constant temperature according to claim 5, wherein: the lamp tube (15) is arranged inside the transparent culture cylinder (5), one end of the lamp tube is connected with the timing device (7) while the other end of the lamp tube is connected with the main control box (8), and the main control box (8) is used for controlling the brightness of the lamp tube (15).
7. The apparatus for cultivating algae at constant temperature according to claim 6, wherein: at least six groups of stop valves (17) are distributed on the water inlet pipeline (10) and the water discharge pipeline (16).
8. The experimental method for the device for cultivating algae at constant temperature according to claim 7, comprising the following steps:
the method comprises the following steps: a main power supply is turned on, tap water is filled into the water pool (1), a main control box (8) is turned on, the main control box (8) displays the water temperature measured by a temperature sensor (13), a water pumping pump (3) arranged outside the water pool (1) is turned on, the water heated in the water pool (1) is conveyed into an interlayer of a transparent culture cylinder (5) through a water inlet pipeline (10), and the water is discharged into the water pool (1) through a water discharge pipeline (16) above the transparent culture cylinder (5), so that the algae is cultured at constant temperature;
step two: the stop valve (17) of the left water pumping pipeline is closed, the stop valve (17) of the water pumping pipeline on the right transparent culture cylinder (5) is opened, water flow passes through the water bath heat insulation layer of the transparent culture cylinder (5), and the left algae culture device and the right algae culture device can be used independently;
step three: algae to be cultured is added into the transparent culture cylinder (5), the temperature of the algae measured by the temperature sensor (13) is displayed by the main control box (8), when the temperature of the algae reaches a certain value, the operation of the heating rod (2) is controlled, and the main control box (8) can independently control the operation of the left culture device and the right culture device;
step four: an air pump (9) is started, one end of the air pump is connected with an aeration head (12) through an air inlet pipeline (14), the other end of the air pump is connected with a flow meter (4) through a hose (11), and the flow meter (4) is adjusted to control the amount of carbon dioxide required by the growth of algae;
step five: and (3) opening a lamp tube (15) arranged in the transparent culture cylinder (5), connecting one end of the lamp tube (15) with a timing device (7), connecting one end of the lamp tube with a master control box (8), and opening the timing device (7) for controlling the brightness time of the lamp tube (15) so as to achieve the optimal growth condition of algae.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114149924A (en) * | 2021-12-03 | 2022-03-08 | 安徽理工大学 | Floating bead flotation harvesting method for microalgae |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114149924A (en) * | 2021-12-03 | 2022-03-08 | 安徽理工大学 | Floating bead flotation harvesting method for microalgae |
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