CN101050419B - Production apparatus for culturing tiny alga and production method - Google Patents

Production apparatus for culturing tiny alga and production method Download PDF

Info

Publication number
CN101050419B
CN101050419B CN 200710107942 CN200710107942A CN101050419B CN 101050419 B CN101050419 B CN 101050419B CN 200710107942 CN200710107942 CN 200710107942 CN 200710107942 A CN200710107942 A CN 200710107942A CN 101050419 B CN101050419 B CN 101050419B
Authority
CN
China
Prior art keywords
microalgae
reactor
production
culture
co2
Prior art date
Application number
CN 200710107942
Other languages
Chinese (zh)
Other versions
CN101050419A (en
Inventor
王逢旦
Original Assignee
王逢旦
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 王逢旦 filed Critical 王逢旦
Priority to CN 200710107942 priority Critical patent/CN101050419B/en
Publication of CN101050419A publication Critical patent/CN101050419A/en
Application granted granted Critical
Publication of CN101050419B publication Critical patent/CN101050419B/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/06Tubular
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/22Transparent or translucent parts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02Stirrer or mobile mixing elements
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/16Vibrating; Shaking; Tilting
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06Nozzles; Sprayers; Spargers; Diffusers
    • C12M29/08Air lift
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00Means for providing, directing, scattering or concentrating light
    • C12M31/10Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M39/00Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms

Abstract

This invention relates to a production apparatus for culturing microalgae. The production apparatus comprises more than one transparent tubular culture reactor units aligned in parallel or alternately parallel. The reactor units are fixed on the base by flanges to constitute the production apparatus. A static mixer, a luminescent device and an electromagnetic vibrator are set at the central axis of each reactor unit. CO2 micro-nozzles are set on the central hollow tube of the static mixer, the diversion blade and the bottom of the reactor unit. The production apparatus has such advantages as low investment, easy operation, special structure, adequate utilization of sunlight, and continuous production, can be widely used in microalgae production.

Description

一种培养微藻的生产装置及其生产方法 A method of culturing microalgae production apparatus and production method of

技术领域。 Technology.

[0001] 本发明涉及微藻培育工程领域,特别是涉及培养微藻的生产装置及其生产方法。 [0001] The present invention relates to microalgae cultivation engineering, particularly to the production apparatus and production method for culturing microalgae. 背景技术 Background technique

[0002] 微藻类自古至今一直生殖在淡水、海水或咸湖水中,特别是例如众所周知螺旋藻是上世纪六十年代在乍得湖地区发现至今,得到迅速发展,只因为该微藻含丰富的蛋白质和营养成份,被成为浓缩营养宝库,被联合国粮农组织(FAO)推荐为21世纪最理想完美的食品,世界各国纷纷竞相生产,目前世界各国产量接近万吨。 [0002] microalgae since ancient times, has been reproductive in freshwater, seawater or salty water, particularly well-known example is the 1960's Spirulina found so far in the area of ​​Lake Chad, have developed rapidly, simply because the microalgae rich in protein and nutrients, known as the treasure house of nutrition concentrated, the United Nations FAO (FAO) recommended as the perfect food in the 21st century the best countries in the world have been competing to produce the current world production of nearly tons.

[0003] 随着世界各国工业生产迅速发展,能源消耗不断增加,人类生存环境日趋恶化,工业CO2的排放量远远超过地球上植物所吸收消耗的CO2,造成气候变暖,严重威胁到人类生存环境。 [0003] With the rapid development of world industrial production, energy consumption is increasing, the deterioration of the human environment, industrial CO2 emissions far exceed the CO2 absorbed by plants on Earth consumed, resulting in global warming, a serious threat to the survival of mankind surroundings. 然而不可再生的能源如天然气、石油及煤炭资源日趋减少,近年来石油价格飞涨, 不得不告诫人们必须寻找可再生的新能源成为世界各国注意焦点,其中含富油微藻引起人们特别关注。 However, non-renewable energy sources such as natural gas, oil and coal resources dwindling in recent years, soaring oil prices, had warned people must find new renewable energy has become the focus of world attention, which contains oil-rich microalgae causing particular concern. 根据有关调查研究表明,目前使用的石油、天然气正是源自史前的生物物质(包括海藻),在高热高压条件下形成的,自然界的生物物质的分解过程特别是持续长达数百万年之久。 According to the research shows that the current use of oil, natural gas is derived from prehistoric biomass (including algae), the decomposition process, the nature of the biological material formed under high pressure conditions, especially high heat lasts up to millions of years Long. 我们今天使用的很多石油都起源于200万年前石炭纪,例如北海海底获取的石油沉积物,部分是由一种已经分解的被称为球石藻的海藻组成。 Many oil we use today originated 200 million years ago Carboniferous, such as North Sea oil deposits acquired, partly from seaweed is called Coccolithophores an already decomposed components. 今天培养微藻,能在数天内实现,有可能成为未来世界各国开发新能源填补即将耗尽的石油及天然气资源。 Today cultivation of microalgae, can be achieved in a few days, the world is likely to become the future development of new energy sources to fill the oil and gas resources will soon run out.

[0004] 目前,世界上培育的微藻(如螺旋藻等)的生产方式仍采用开放式_露天大池生产系统进行生产,尽管生产简单,投资低,生产管理技术含量低,但这种生产方式存在诸多缺陷,例如易于生物染污,即来自水源、大气、环境、操作等造成的污染,并受气候条件严重制约,使微藻生产的产品质量及稳定生产带来极其不利影响,特别光照少多雨地区以及具有严冬季节长的地区无法全年正常生产,直接限制了微藻的培养与开发。 [0004] Currently, the world cultivated microalgae (such as spirulina) mode of production is still an open open-air pool _ production system for production, although production is simple, low investment, low-tech production management, but this mode of production there are many defects, such as ease of biological stain, which have extremely adverse impact on product quality and stable production from the pollution caused by water, air, environment, operation, and is subject to weather conditions severely restrict the production of microalgae, particularly less light wet areas as well as the long winter season the region has not normal annual production, directly limit the cultivation and development of microalgae. 进入上世纪八十年代以来,世界各国的微藻生产开发商(螺旋藻)及生产工程专家们为了克服上述制约生产发展的因素,开发多种封闭式培育微藻(如螺旋藻)的装置和方法,例如管道式结构光生物反应器(TorzillO等人,1993)及CN1475558A公开微藻(螺旋藻)的反应器系统,采用人工光照培育的双塔结构,这些装置主要用来生产食品级螺旋藻,投资大,耗能高,成本高,不易长期工业化生产,至于对含富油微藻的培育装置及生产方法尚未见有报道。 Since the beginning of the 1980's, the developer of the world production of microalgae (spirulina) and production engineering experts in order to overcome the above-mentioned factors restricting the development of production, development of a variety of closed-end device cultivate microalgae (Spirulina) and methods, such as pipe structure photobioreactors (TorzillO et al., 1993) and CN1475558A discloses microalgae (Spirulina) reactor system, using a double column configuration artificial light cultivation, which means mainly used to produce food-grade Spirulina , investment, high energy consumption, high cost, difficult long-term industrial production, as the cultivation apparatus and process for producing the oil-rich microalgae has not been reported.

发明内容 SUMMARY

[0005] 本发明的目的在于克服上述现在技术存在缺陷及不足,开发一种投资低,操作简单,结构独特,能充分利用太阳光,又能全天候生产的培养微藻的生产装置及其生产方法。 [0005] The object of the present invention to overcome the above-described operation is simple, unique structure, can make full use of sunlight, but also weather production apparatus and production method for producing microalgae culture now technical defects and deficiencies, the development of a low investment, .

[0006] 本发明提供的培育(或称育养)微藻的生产装置,其特征在于包括至少一个(透明管式培养)反应器单元装置(简称反应器)1,平行或交替平行间隔排列,例如包括直立或倾斜平行或交替平行间隔排列,分别通过法兰11固定在地面底座10上构成系列生产装置;每个透明管式培养反应器单元装置由透明材料制成管式或圆筒体,直径25〜100cm,高2〜6米,优选2〜4米,反应器顶端中心设有排氧出气口7及上端部进水口13,底端中心设有CO2进气口6,下底部设有微藻培养液放出口8 ;在管式培养反应器单元装置的中心轴线区设有静态混合器2、光照装置3、以及电磁振荡器9,所述静态混合器为中空管叶片式静态混合器或螺带式静态混合器,设在反应器中心轴线区,它由中心空管与交替设置4-12个导流叶片4 (视反应器高度而定)组成,导流叶片为中空导流叶片, [0006] Cultivation of the present invention provides (or upbringing) microalgae production apparatus, characterized by comprising at least a (transparent tube culture) reactor unit of the device (the reactor) 1, parallel or alternating parallel spaced, includes, for example upright or inclined parallel or alternating parallel spaced, respectively, by a flange 11 fixed to the ground base 10 constituting the range of production means; culture of each transparent tubular reactor unit means a tubular or cylindrical body made of a transparent material, diameter 25~100cm, 2 ~ 6 meters high, preferably 2 ~ 4 meters, the center of the top of the reactor is provided with an oxygen discharge outlet 7 and the upper portion of the water inlet 13, the bottom end of the central air inlet 6 is provided with CO2, under a bottom microalgae culture liquid discharge outlet 8; culturing the central axis of the tubular zone of the reactor unit of the device is provided, and the illumination device 3, and an electromagnetic oscillator 9, the static mixer is a hollow tube-type static mixing vane static mixer ribbon or a static mixer, is provided in the region of the central axis of the reactor, which (as the height of the reactor) by a central hollow tube provided with alternate guide vanes 4 composed of 4-12, a hollow guide vane guide blade, 导流叶片上设有若干个CO2出口微喷嘴(孔),CO2可通过微喷嘴连续向反应器内输送CO2,通过嵌合与反应器上下法兰相接固定在反应器中心轴线区中央。 CO2 outlet provided with a plurality of micro nozzles (holes) on the guide vanes, CO2 CO2 continuously conveyed into the reactor through a micro nozzle, fixed to the contact zone of the reactor central axis through the center of the vertical reactor fitted with a flange. 中心空管上、导流叶片上及反应器底部上设有CO2出口微喷嘴,CO2通过中心空管、反应器底上及导流叶片上微喷嘴连续供给反应器内微藻所需的CO2 ;上端部位处设有氧气和CO2通道孔12 ;光照装置设在静态混合器中心空管中心与反应器顶法兰嵌合相连;电磁振荡器设在反应器底部静态混合器周围。 Air traffic control center, provided with guide vanes and the bottom of the reactor outlet micronozzles CO2, CO2 air through the central tube, the bottom of the reactor and the nozzle guide vane micro-reactor is continuously fed to the desired CO2 microalgae; provided at the upper portion of the oxygen and CO2 passage hole 12; illumination means provided in the air traffic control center of the central static mixer reactor with a top flange connected to the fitting; an electromagnetic oscillator provided in the bottom of the reactor around the static mixer.

[0007] 本发明提供的培养微藻的生产方法,包括在培养微藻的生产装置或培养微藻管式反应器单元装置中,装入接种微藻液为反应器容积1/100至1/4,其余为水,如果需要还可加入微藻培养过程中加快生长速度的有机和无机营养物质、微量元素及促进含油微藻快速生长的激素,连续通入来自电厂或其它CO2源,使微藻处于磁场及微振动下,由于电厂CO2源为热CO2源,可用来调节培养温度,培养温度为25〜45°C,培养时间1-10天,充分利用太阳光使微藻在阳光下迅速繁殖,如果需要夜间连续生产可采用光照装置。 [0007] The present invention provides a method for producing microalgae culture, comprising a tubular reactor unit microalgae culture device of microalgae culture or production means, is charged with a liquid inoculum microalgae 1/100 to 1 reactor volume / 4, the balance being water, may also be added if desired to speed up the process of microalgae culture of organic and inorganic nutrients, trace elements and hormones promote the rapid growth of the oleaginous microalgae growth rate, was continuously fed from power plants or other source of CO2, so that the micro- micro-algae in the magnetic field and vibration, since the CO2 source is a thermal power plant CO2 source can be used to adjust the temperature of the culture, the culture temperature of 25~45 ° C, culture time 10 days, full use of sunlight in the sun quickly microalgae reproduction, may be employed if desired nighttime illumination apparatus for continuous production. 视微藻培养液浓度不定期收集微藻,即使反应器中培养过的微藻液排出3/4至99%经培养液放出口采集,经离心去水或经喷雾干燥等制成微藻粉或经高压澎胀破微藻壁提油成分。 From time to time depending on the concentration of microalgae culture was collected microalgae, even if the culture reactor through the liquid discharge microalgae 3/4 to 99% discharge outlet broth was collected, centrifuged to water or the like made of spray-dried microalgae or by high pressure burst Peng microalgal oil component mentioned wall.

[0008] 按照本发明提供的培养微藻的生产装置及培养微藻的生产方法中,所述微藻包括来自淡水微藻,咸湖水微藻或海水微藻等,例如绿藻、蓝藻、硅藻、红球藻、雨生红球藻、紫球藻等,优选为含油脂高的微藻如绿藻、红球藻、雨生红球藻、硅藻或蓝球藻等。 [0008] The production of microalgae culture apparatus according to the present invention provides methods of culturing and production of microalgae, said microalgae comprising microalgae from fresh, salt lake water or seawater microalgae and other algae, such as green algae, cyanobacteria, silicon algae, Haematococcus algae, Haematococcus alga, Porphyridium the like, preferably with high fat microalgae such as green algae, Haematococcus, Haematococcus algae, diatoms, or basketball algae. 接种微藻液通常为反应器容积的1/100至1/4,其中微藻液可以无需每次外来接种液,只需每次采集培养过的微藻液排放反应器内藻液的3/4至99/100,剩于1/100至1/4为下次培养液中的接种微藻液。 Microalgae were inoculated reactor volume is usually 1/100 to 1/4, wherein the microalgae liquid may each alien without inoculum, cultured algal only liquid 3 within each acquisition discharge reactor liquid alginate / 4 to 99/100, 1/100 to 1/4 of the remaining inoculum liquid Save microalgae culture solution.

[0009] 本发明微藻培养中所需CO2气体来自电厂或其它CO2源排出经处理除去气体中所含不利于微藻繁殖的杂质气体和微粒(包括粉尘)物质,(即符合微藻生长繁殖所要求的CO2气体),因为来自电厂的CO2资源丰富,价廉,而且是热的CO2,可通过热CO2或加热CO2来调节培养温度,培养温度一般25〜45°C .根据不同微藻采用不同的培养温度。 [0009] The present invention is microalgae culture in the desired plant or other CO2 gas from the CO2 source discharge treated to remove impurities detrimental to the microalgae reproduce gases and particles (including dusts) species contained in the gas, (i.e., in line with the growth and reproduction of microalgae CO2 gas desired), since the plant resources from the CO2 rich, inexpensive, and hot CO2, the culture temperature may be adjusted by heat or heating CO2 CO2, the culture temperature is usually 25~45 ° C. depending on the use of microalgae different culture temperatures.

[0010] 所述透光材料是采用透光性好的玻璃,加强玻璃如高硼硅加强玻璃,有机玻璃,透明性聚合物材料如透明性聚乙烯、聚丙烯、聚氯乙烯、聚酯、聚碳酸酯或一氧化碳与脂肪不饱和化合物的共聚物等。 [0010] The light transmissive material is a good use of the light-transmitting glass, such as borosilicate glass reinforcing strengthening glass, plexiglass, transparent polymeric material such as a transparent polyethylene, polypropylene, polyvinyl chloride, polyester, polycarbonate or a copolymer of carbon monoxide with an unsaturated aliphatic compound and the like.

[0011 ] 在所述培养微藻的生产装置中,管式培养微藻反应器单元装置可以独立单独生产微藻或者多个例如平行间隔排列或交替平行间隔排列或向阳光倾斜式排列构成系列生产线,各个单元装置的氧气排气口都可连接在一个管线上,作回收或放空处理。 [0011] In the production of microalgae culture apparatus, a tubular reactor unit microalgae culture device of microalgae can be produced separately, or independently, for example, a plurality of parallel spaced or spaced alternating parallel or inclined to the sun, arranged in series production line of formula oxygen exhaust port means of each unit may be connected in a line, for venting or recycling process. 同时这种排列可充分利用自然太阳光使培养微藻反应器单元装置全方位都处于太阳光照射之下,能充分利用太阳光。 While this arrangement may make full use of natural sunlight reactor unit microalgae culture apparatus are under full sunlight, can make full use of sunlight. 与此同时,在太阳光不足的天气及晚间可采用光照装置同样能以自然太阳光一样光照使微藻快速繁殖。 At the same time, the lack of sunlight weather and evening light fixtures can be equally able to natural sunlight as light rapid propagation of microalgae. 微藻在正常生长条件下,每1-5小时即可繁殖一代,微藻可以在太阳光照射下,于水中吸收CO2就能迅速繁殖,为了使微藻繁殖更迅速,还可以在水中加入适量有机及无机物质(含氮、ρ、κ)、微量元素及富油含量的生长激素(都是市售产品)。 Microalgae under normal growth conditions, each breeding generation to 1-5 hours, microalgae may be the sunlight, absorption of CO2 in the water can be rapidly, in order to make more rapid propagation of microalgae, can also be added in an appropriate amount of water organic and trace element content of the oil-rich growth hormone inorganic substances (nitrogen, ρ, κ) and (all commercially available product). 在反应器单元装置内设静态混合器,例如在中心轴线区设有如图1、2所示的导流叶片(或简称叶片)式静态混合器如浆式或螺带式导流叶片,其中导流叶片交替排列在中心空管上(以嵌合或点焊连接),分布于整个反应器空间中,导流叶片为中空导流叶片,并在导流叶片上设有微喷嘴(孔),CO2可通过此孔不断向培养液中补充CO2,使CO2在整个反应器内浓度均一,或者当反应器单元装置直径在SiTlOOcm,可在中心轴线区光照装置周围空间设3-6个带有导流叶片的静态混合器(图1所示的类型静态混合器)。 In the reactor unit of the device equipped with a static mixer, for example, it is provided in the region of the central axis of the guide vane in FIG (or simply, blades) represented by the formula 1 or a static mixer, such as paddle ribbon guide vanes, wherein the guide blades are alternately arranged on the central air flow tube (connected to chimeric or spot welding), distributed throughout the reactor space, the hollow guide vanes of the guide vane, and a micro-nozzles (holes) in the guide vane, CO2 can be continuously added through the hole CO2 into the culture broth, so that the CO2 concentration in the uniform throughout the reactor, when the reactor unit of the device or diameter SiTlOOcm, may be provided with 3-6 light guide means in the space around the central axis of the region the static mixer vanes (type static mixer shown in FIG. 1). 在静态混合器的中心空管上或导流叶片上及反应器底部上设有CO2单向出口(微)喷嘴,CO2经中心空管、导流叶片及底部微喷嘴进入反应器,上升过程中受导流叶片作用于整个反应器空间中,使CO2与微藻液均勻充分混合分布,使整个反应器中CO2分布浓度接近相同。 ATC on the central static mixer provided with unidirectional CO2 or outlet guide vanes and the bottom of the reactor (micro) nozzles, CO2 through the central air duct, the guide vane and the bottom of the nozzle into the micro-reactor, rising process by acting on the guide vane throughout the reactor space, so CO2 microalgae was uniformly mixed distribution, so that the concentration of CO2 distribution approximately the same throughout the reactor. 并以零剪切湍动。 Zero shear and turbulence. 在底部设电磁振荡器或石英磁性振荡器使反应器内的微藻培养液中的微藻处于磁场及微振动中,极大有利于微藻的接触流动与繁殖以及消除微藻有可能附于壁上。 An electromagnetic oscillator provided in the bottom of the magnetic oscillator or quartz in the reactor microalgae culture solution in algae and micro-vibration field, greatly favor the flow in contact with the propagation of microalgae and algae may be appended to eliminate wall. 所述光照装置设置在静态混合器中心空管管内,发出类似于自然太阳光源和最适宜微藻生长,使整个反应器空间、频率和波长全方位处于类太阳光照下,微藻能吸收到几乎相同于太阳光的或更有益光照能量, 在相同光照条件下繁殖:由于它处于中心空管内,避免了光照装置直接接触微藻,产生受局部过热不利于生产繁殖或死亡。 The illumination means disposed in the central hollow pipe static mixer, similar to natural sun light and emit the most suitable algae growth, the entire reaction space, frequency and wavelength is in full sun-light, absorbs almost microalgae identical to sunlight or more beneficial light energy propagated under the same lighting conditions: Since it is the central empty tube, avoiding direct contact with the light emitting devices microalgae, to produce local overheating is not conducive to the production by reproduction or death. 光照装置与中心空管之间存在CO2通道空间,可被CO2带走产生的热量。 CO2 passage space exists between the illumination means and the air traffic control center, which may be taken away heat generated CO2.

[0012] 本发明提供的培养微藻的生产装置及方法中,还设有配套高压喷枪水清洗设备及消毒设备,在生产过程中万一产生反应器沾壁与结污或污染,可采用高压喷枪水清洗设备或进行消毒,保证微藻生长。 [0012] Production of microalgae culture apparatus and method of the present invention is provided, is also provided with supporting high-pressure water spray gun cleaning equipment and disinfection equipment, in case of generation reactor wall fouling soiled or contaminated, high pressure may be employed in the production process cleaning lance device or sterilized water, to ensure the growth of microalgae.

[0013] 本发明提供的培养微藻的生产装置及其培养微藻的生产方法的特点为: [0013] Production of microalgae culture apparatus of the present invention provides a method for producing microalgae culture and its characteristics are as follows:

[0014] 1.培养微藻的生产装置结构独特,操作简单方便,占地面积小,能充分利用太阳光,节省电能,降低生产成本。 Unique [0014] 1. Production of microalgae culture apparatus structure, simple operation, small footprint, can make full use of sunlight, to save energy, reduce production costs.

[0015] 2.在生产装置中由于采用静态混合器及电磁振荡器,培养微藻液处于磁场及微振动条件下均勻全方位以相同条件下迅速繁殖,没有死角。 [0015] In the production apparatus 2. As a result of a static mixer and electromagnetic oscillator, microalgae culture solution in a uniform full multiply rapidly in the magnetic field under the same conditions and the micro-vibration conditions, it is not dead.

[0016] 3.采用封闭结构以及能全天候生产完全控制外来环境生物异物的染污,确保微藻能正常迅速繁殖生长。 [0016] 3. The use of all-weather enclosed structure and full control over the production of bio-stained exotic environment of foreign bodies, to ensure that algae can multiply rapidly growing normally.

[0017] 4.采用电厂的CO2气源和其它CO2源,变废为宝,通过静态混合器中心空管上设置的CO2喷嘴及导流叶片上喷嘴以及底部微喷嘴使CO2在反应中分布均勻,上下浓度均一,并通过此热CO2源能有效调节培养温度确保全天候的培养温度。 [0017] 4. The use of plants and other CO2 source of CO2 gas source, recycling Po, and the nozzle guide vane CO2 through the central static mixer disposed ATC nozzle and a bottom nozzle so that the micro-distribution in the reaction CO2 , uniform concentration and down, and by this the heat source of CO2 can effectively regulate the temperature of the culture to ensure weather cultivation temperature.

[0018] 5.本发明的生产装置由于科学合理设计,不易产生的结痴和沾壁,万一出现此现象,可打开上法兰用高压水喷枪极易清洗及进行彻底消毒。 [0018] The production apparatus of the present invention, since the scientific and rational design, less likely to stick to the wall and crusting, if this phenomenon occurs, the flange can be opened with a high-pressure water lance easy to clean and disinfect thoroughly.

[0019] 6.本发明的生产装置及方法既可以以培养微藻反应器单元装置进行单独生产,又能多个或上百单元装置进行集约化系列生产,保养维修简单。 [0019] 6. The producing apparatus and method of the present invention may be in a cell culture reactor microalgae production apparatus separately, but a plurality of units or devices intensification hundred series production, maintenance simple.

[0020] 7.只要进行一次接种微藻,后续生产无需再接种微藻 [0020] 7. microalgae as long as vaccination, revaccination without the subsequent production of microalgae

附图说明 BRIEF DESCRIPTION

[0021] 图1为培养微藻反应器单元装置的结构示意图 [0021] FIG. 1 is a reactor unit for culturing microalgae structural diagram of apparatus

[0022] 图2为静态混合器螺带导流叶片结构示意图 [0022] FIG. 2 is a schematic view of guide vane structure with a static mixer spiro

[0023] 图3为静态混合器浆式导流叶片结构示意图 [0023] FIG. 3 is a schematic view of the structure of a paddle guide vane static mixer

5具体实施方式 5 DETAILED DESCRIPTION

[0024] 本发明用下列具体实施方案结合附图进一步说明本发明;但本发明的保护范围并不限于下列具体实施方案。 [0024] The present invention is further illustrated by the following in conjunction with the accompanying drawings of the present invention a particular embodiment; however, the scope of the present invention is not limited to the following specific embodiments.

[0025] 实施例1 [0025] Example 1

[0026] 培养微藻的生产装置为培养微藻反应器单元装置,反应器采用透明聚乙烯圆筒形带法兰的反应器1,真径80cm,高2. 5米,通过法兰11固定在地面底座10上。 [0026] culturing microalgae production means is means for culturing microalgae reactor unit, the reactor transparent polyethylene flanged cylindrical reactor 1, the true diameter of 80cm, 2. 5 meters high, fixed by a flange 11 the base 10 on the ground. 在反应器中心轴线区设有如图1所示的浆式导流叶片静态混合器2,浆式导流叶片4为8片,为中空式, 设有微喷嘴,交替嵌合在中心空管上,中心空管中设有光照装置3,CO2出口喷嘴(单问)5, 交替设在中心空管、叶片及底部上,中心空管顶上部设有氧气及CO2通道口12与顶部氧气出口7相通,底部为CO2进气口6,静态混合器中心空管底部反应器内设电磁振荡器9,底部一侧设培养液放出口8及上部进水口13。 In the reactor zone with the center axis 1 in a static mixer paddle guide vanes 2, 4 is a paddle guide vanes 8, a hollow type, provided with micro nozzles are alternately fitted in a central hollow tube , a central hollow tube is provided with illumination means. 3, CO2 outlet nozzles (mono Q) 5, are alternately arranged in the air traffic control center, and bottom leaf, the top portion of the central tube is provided with air oxygen and CO2 passage opening 12 and the top of the oxygen outlet 7 communicating, CO2 bottom inlet port 6, the static mixer ATC center bottom of the reactor equipped with an electromagnetic oscillator 9, the bottom side of the discharge outlet 8 provided broth and the upper inlet 13. 在培养开始前,通过反应器顶部法兰加入反应器容积1/10接种红球藻液,杀菌水9/10,密封法兰,启动电磁振荡器,使反应器内培养液处于微振动及电磁场下,来自电厂经处理过的CO2由底部CO2进气口6进入中心空管经CO2出口喷嘴进入反应器培养液中,通过CO2进入量调节温度在30〜35°C,恒定后连续通入CO2,在培养过程中产生氧气及未被微藻吸收的CO2经通道12从排氧出气口排放,培养3天后(晚间打开光照装置)测定微藻液浓度附合要求后,放出90%微藻培养液,留下1/10培养液作为下次培养接种微藻培养液,放出培养液经过滤除水再经喷雾干燥得藻粉。 Before beginning of the culture, inoculation 1/10 volume Haematococcus was fed through the reactor top flange of the reactor, 9/10 sterilizing water, the sealing flange, and drives the electromagnetic oscillator, the culture liquid in the reactor is in micro-vibration and electromagnetic fields next, the treated plants from CO2 from the bottom of CO2 into the air inlet 6 into the central hollow tube through the outlet nozzle into the reactor CO2 broth by adjusting the amount of CO2 entering temperature of 30~35 ° C, CO2 was continuously fed after constant generating oxygen and CO2 is not absorbed by the microalgae culture in the passage 12 during the discharge oxygen from exhaust gas outlet, the culture (open evening illumination means) measured microalgae concentration meets the requirement, 90% released after 3 days of culture the microalgae liquid, leaving the next 1/10 of the culture broth was inoculated as microalgae culture liquid, culture liquid discharged through the filtered water and then spray-dried to obtain algal flour.

Claims (5)

  1. 一种培养微藻的生产装置,其特征在于包括至少一个透明管式培养反应器单元装置(1),平行或交替平行间隔排列,分别通过法兰(11)固定在地面底座(10)上构成系列生产装置;每个管式培养反应单元装置为透明材料制成的管式或圆筒体,直径25~100cm,高2‑6米,反应器顶端中心设有排氧出气口(7)及上端部进水口(13),底端中心设CO2进气口(6),下底部设微藻培养液放出口(8);在单元装置的中心轴线区设静态混合器(2),光照装置(3)以及电磁振荡器(9),所述静态混合器由中心空管与交替设置4~12个导流叶片(4)组成,通过嵌合与反应器上下法兰(11)相接固定在反应器中心轴线区中央;中心空管、导流叶片及反应器底上均匀设有若干CO2出口微喷嘴及上端部处设有氧气和CO2通道(12);光照装置设在静态混合器中心空管中心与反应器顶法兰嵌合相连,电磁振荡器设在反 Production means for culturing microalgae, comprising culturing at least one transparent tubular reactor unit means (1), parallel or alternating parallel spaced, are fixed to the ground constituting the base (10) through the flange (11) series production plant; a tubular reactor or a cylinder unit of the device is made of transparent material to each culture tube, the diameter of 25 ~ 100cm, 2-6 meters high, the center of the top of the reactor is provided with an oxygen discharge outlet (7) and an upper inlet portion (13), a bottom end centers CO2 intake port (6), provided at the bottom of the microalgae culture liquid discharge outlet (8); provided a static mixer (2), the central axis of the illumination device unit of the device region (3) and an electromagnetic oscillator (9), the static mixer by a central hollow tube provided with alternate guide vanes 4 to 12 (4), with the fixed contact by a vertical reactor fitted with a flange (11) in the central region of the central axis of the reactor; uniformly provided on the central empty tube, the guide vane and the bottom of the reactor and a plurality of micronozzles CO2 outlet provided at the upper portion of the oxygen and CO2 passage (12); illumination means provided in the center of a static mixer ATC center fitted with a flange connected to the top of the reactor, an electromagnetic oscillator provided in trans 器底部静态混合器周围。 Around the bottom of the static mixer device.
  2. 2.根据权利要求1的培养微藻的生产装置,其特生在于所述透明材料为玻璃,有机玻璃或透明性聚合物;所述导流叶片为中空导流叶片,在叶片上设若干个微喷嘴。 2. The production of microalgae culture apparatus according to claim 1, wherein Bendtsen which the transparent material is glass, organic glass or a transparent polymer; said hollow guide vane guide vanes disposed in the plurality of blades micronozzles.
  3. 3. 一种用权利要求1的生产装置培养微藻的生产方法,其特征在于包括在培养微藻的生产装置中,装入接种微藻液为反应器容积百分之一至四分之一,其余为水或含有有机或无机营养物质、微量元素及激素的水;连续通入来自电厂或其它CO2源,使微藻培养液处于磁场及微振动下,培养温度于25〜45°C,培养时间1-10天,白天直接用自然太阳光,晚间使用光照装置供给微藻所需光能量,达到培养浓度后排出微藻液四分之三至99%经培养液放出口采集,经离心去水或喷雾干燥得藻粉。 A production method for producing a device as claimed in claim 1 for culturing microalgae, comprising the production of microalgae culture apparatus, the volume of liquid algae inoculum was charged to one quarter of one percent of the reactor the remainder being water or an organic or inorganic nutrients, trace elements and hormones water; continuously fed from a power plant or other source of CO2, microalgae and the culture solution in a micro-vibrating magnetic field, culture temperature at 25~45 ° C, 1-10 days culture, the direct use of natural sunlight during the day, night illumination apparatus using the light energy required for the supply of microalgae, the culture reached after discharge microalgae concentration was 99% over three-quarters of the discharge outlet broth collected by centrifugation to water or spray drying algae powder.
  4. 4.根据权利要求3的培养微藻的生产方法,其特征在于所述微藻为绿藻、蓝藻、硅藻、 红球藻、雨生红球藻或紫球藻。 4. The method for producing microalgae culture according to claim 3, wherein the microalgae is a green algae, blue-green algae, diatoms, Haematococcus, Haematococcus algae or Porphyridium.
  5. 5.根据权利要求4的培养微藻的生产方法,其特征在于所述微藻为红球藻或雨生红球藻。 The culturing microalgae production process according to claim 4, wherein the microalgae is pluvialis Haematococcus or rain.
CN 200710107942 2007-05-18 2007-05-18 Production apparatus for culturing tiny alga and production method CN101050419B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 200710107942 CN101050419B (en) 2007-05-18 2007-05-18 Production apparatus for culturing tiny alga and production method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 200710107942 CN101050419B (en) 2007-05-18 2007-05-18 Production apparatus for culturing tiny alga and production method

Publications (2)

Publication Number Publication Date
CN101050419A CN101050419A (en) 2007-10-10
CN101050419B true CN101050419B (en) 2010-12-29

Family

ID=38782019

Family Applications (1)

Application Number Title Priority Date Filing Date
CN 200710107942 CN101050419B (en) 2007-05-18 2007-05-18 Production apparatus for culturing tiny alga and production method

Country Status (1)

Country Link
CN (1) CN101050419B (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0919934A2 (en) * 2008-10-24 2015-08-11 Bioprocess H20 Llc Container, method and system for cultivating microorganisms, and method for cultivating free oxygen during cultivation of microorganisms
CN101760418B (en) * 2008-12-17 2014-01-01 新奥科技发展有限公司 Connecting system for parallel pipe type photobioreactor
CN101665765B (en) 2009-09-23 2012-05-30 王逢旦 Continuous production device for culturing Microalgae by closed piston flows and production method thereof
KR101382989B1 (en) * 2011-09-02 2014-04-08 현대자동차주식회사 Photo-bioreactor for culturing micro algae
CN102517218B (en) * 2011-12-28 2013-05-29 广东海洋大学 Method for culturing microalgae on sea surface
CN102657071B (en) * 2012-05-29 2013-09-04 中国水产科学研究院渔业机械仪器研究所 Method for culturing phytoplankton with predominant chlorophyta
CN102943028B (en) * 2012-11-26 2015-04-08 中国科学院广州能源研究所 High-pressure micro-jet carbon supplementing system for microalgae cultivation of raceway pond
CN103070096B (en) * 2012-12-13 2014-04-02 浙江海洋学院 Umbrella-shaped juvenile fish protection device
MD4372C1 (en) * 2014-03-04 2016-04-30 Государственный Университет Молд0 Installation for cultivation of microalgae
CN106861480B (en) * 2015-12-10 2019-10-29 中国石化工程建设有限公司 Static mixer
CN106861479B (en) * 2015-12-10 2019-10-29 中国石化工程建设有限公司 Static mixer
CN106675992B (en) * 2017-01-17 2019-02-19 杭州元点生物科技有限公司 A kind of stirring system of control microdisk electrode circulation

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN201046966Y (en) 2007-05-18 2008-04-16 王逢旦 Production device for culturing microalgae

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN201046966Y (en) 2007-05-18 2008-04-16 王逢旦 Production device for culturing microalgae

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
刘娟妮等.微藻培养中光生物反应器的研究进展.食品科学第27卷 第12期.2006,27(12),第772-777页,尤其第2节.

Also Published As

Publication number Publication date
CN101050419A (en) 2007-10-10

Similar Documents

Publication Publication Date Title
Wang et al. Closed photobioreactors for production of microalgal biomasses
Ozkan et al. Reduction of water and energy requirement of algae cultivation using an algae biofilm photobioreactor
AU2007313669B2 (en) Closed photobioreactor system for production of ethanol
JP4165715B2 (en) Multiple photobioreactor and photosynthetic microorganism culture method using the same
US8268601B2 (en) Continuous-batch hybrid process for production of oil and other useful products from photosynthetic microbes
Watanabe et al. Photosynthetic performance of a helical tubular photobioreactor incorporating the cyanobacterium Spirulina platensis
Sato et al. Invention of outdoor closed type photobioreactor for microalgae
Pulz Photobioreactors: production systems for phototrophic microorganisms
Morita et al. Investigation of photobioreactor design for enhancing the photosynthetic productivity of microalgae
US20110070632A1 (en) Photo bioreactor and cultivation system for improved productivity of photoautotrophic cell cultures
CN101838606B (en) Airlift loop bioreactor through microalgae photoautotrophic-photoheterotrophic coupling for carbon emission reduction in sewage treatment
US20130109008A1 (en) Method and apparatus for growing photosynthetic organisms
AU2006324198B2 (en) A carbon supply device for cultivating miro algae in large and its application method and use
Miyamoto et al. Vertical tubular reactor for microalgae cultivation
DK2501795T3 (en) Accordion-bioreactor
CN101549932B (en) Integrated production method of organic sewage/waste residue treatment, microalgae culturing and oil refining
CN101368193B (en) Process for preparing fine algae cultivation coupling biological diesel oil refining
CN1201826A (en) Spirulina light illuminating tower-tray optic bio-reactor culturing system and its controlling method
CN100587054C (en) Novel multi-section flat type photo-bioreactor
CN102766578A (en) Cultivating and producing method for haematococcus pluvialis
US8323958B2 (en) Closed photobioreactor system for continued daily in situ production of ethanol from genetically enhanced photosynthetic organisms with means for separation and removal of ethanol
CN102365356A (en) Photosynthetic reactor for cultivating microorganisms, and method for cultivating microorganisms
CN101280271A (en) Production unit for microalgae industrialization and method for producing microalgae
Muller-Feuga et al. Comparison of artificial light photobioreactors and other production systems using Porphyridium cruentum
CN1317379C (en) Pipeline photobioreactor for scale culture of microalgae

Legal Events

Date Code Title Description
C06 Publication
C10 Request of examination as to substance
C14 Granted
C17 Cessation of patent right