CN101643700B - Algae growth system device with two-step photobiologic reactors - Google Patents
Algae growth system device with two-step photobiologic reactors Download PDFInfo
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Abstract
The invention relates to an algae growth system device with two-step photobiologic reactors, which comprises a primary photobiologic reactor, a secondary photobiologic reactor, an algae-liquid separator and a detection control system, wherein the algae-liquid separator is arranged between the primary photobiologic reactor and the secondary photobiologic reactor; after being filtered by a filter at the filter opening of the algae-liquid separator, an algae-liquid transmission pipeline is connected with a mixed algae-liquid storage tank; and the algae-liquid transmission pipeline is connected with the sealed end of the secondary photobiologic reactor. Since the primary photobiologic reactor and the secondary photobiologic reactor are independent, the device can effectively avoid the contradictions between the algae growth and the enriched lipid in the photobiologic reactor to provide convenience for respectively using special culture solutions capable of promoting the algae growth and the lipid enrichment, and can respectively maximize the output of the algae containing proteins and carbohydrates and the output of the algae containing lipids in different photobiologic reactors.
Description
Technical field
A kind of algae growth system device with two-step photobiologic reactors; Belong to the algae bio technical field; Relate to a kind of marine alga that produces high yield of biomass and high biomass content, and can carry out the duct type bioreactor marine alga growth system device that marine alga is cultivated continuously.
Background technology
Along with global economy is fast-developing, energy-output ratio increases day by day in the worldwide, and the resource of traditional energy is very limited, and biomass energy more and more receives people's concern as a kind of substitute energy.Research shows that a lot of marine alga oleaginousness are all 20%～50%, and the oleaginousness of some algae (dry mass) can reach 77%, can surpass more than 30 times of ground oil crops (comparing with the soil of unit surface) with marine alga production biofuel.Because algal grown is to utilize carbonic acid gas as carbon source, the carbonic acid gas of algae in can mass consumption factory boiler waste gas in process of growth, the residuum that algae is extracted behind the algal oil is rich in fat and protein, also can be used as animal-feed.Therefore, utilizing industriallization algae production equipment, obtain the needed algae raw material of scale operation biofuel, is that present various countries scientist generally acknowledges the final plan that solves energy substitution.
Nitrogen is the staple that constitutes plant protein, and growth and development of plants is played an important role, and is and one of the closest nutritive element of output.Discover: nitrogen concentration has bigger influence to algal grown and lipid content in the nutritive medium, improves nitrogen concentration and can promote algal grown to increase the increase of protein and glucide, reduces nitrogen concentration and can promote the intravital lipid content of algae to improve.Under the condition that nitrogenous source lacks, dry weight, chlorophyll and the protein contnt of grape algae are obviously descended, but carrotenoid, hydrocarbon polymer and lipid increase, nitrogen hunger can make the content of lipid increase by 1.6 times.Through prolonging the incubation time of frustule under the nitrogen stress state, make the content of triglyceride of frustule improve, can obtain the higher product liquid of lipid content.Research also confirms to chlorella grease enrichment culture, and the average growth velocity of chlorella of adding nitrogenous source is all greater than the algae liquid that does not add nitrogenous source, and under the situation of not adding nitrogenous source, the fat content of marine alga is all maximum, and has reached 45%.The natural mechanism that changes the algae lipid metabolism is the emergency reaction that produces in default of nitrogenous source; Though lack the generation that nitrogen can suppress alga cells growth cycle and nearly all cellular constituent; But make the algae of nitrogen hunger in cell, accumulate lipoid substance, lipid synthetic ratio is still very high.
Alga cells accumulation is greasy, and what are closely related with culture condition, and it is the greasy key factor of high yield that sufficient and other nutritive ingredients of carbon source lack.Algae produces lipid can be divided into two stages, i.e. algae protein matter and glucide propagation phase and lipid accumulation phase, two stage C/N ratios require different.The effect of nitrogenous source is to promote the cell growth, therefore cultivates and requires low ratio of carbon to ammonium early stage, can obtain a large amount of marine algas, and the produce oil stage requires high C/N ratio, makes marine alga accumulate more adipose.
The closed conduit bioreactor of various countries seaweed bio diesel oil company employing is now produced the marine alga that directly is used to produce algal oil, obtains high yield for promoting marine alga to grow on the one hand, needs to increase the nitrogen concentration in the bioreactor algae liquid; On the other hand for improving the alginic cell oleaginousness; Need reduce the nitrogen concentration in the algae liquid again; Force marine alga under the state of nitrogen hunger with cell paste in more protein convert lipid into glucide, want the interior nutrient solution of a bioreactor to satisfy these two kinds of different requirements simultaneously like this and just have contradiction.
Be two contradictions of marine alga growth and lipid-rich in the solution bioreactor, the U.S. has the investigator to propose bioreactor is divided into two portions, obtains the culture scheme of different seaweed biomass component contents respectively.U.S. Ramus; Joseph, " Algae, the biopolymer production " patent (patent No.: 4236349) of S.1980 obtaining the authorization in year; Its principle is marine alga to be cultivated be divided into two stages; The marine alga that fs cultivates is accomplished in first bioreactor, in this bioreactor, can constantly supply nitrogenous nutritive substance, to improve seaweed biomass output.After marine alga reaches results density; The marine alga of a part can be transformed into subordinate phase continuation cultivation in the bioreactor; In the bioreactor of subordinate phase,, make the marine alga quickening speed of growth and nitrogen element in the mass consumption algae liquid through increasing light-receiving area and the light income (sunshine or artificial lighting) of bioreactor; Thereby the environment of making a nitrogen stress makes marine alga get into the old and feeble stage, can increase the output of alginic cell high polymers like this.Be transferred to the individual bioreactor of subordinate phase by the marine alga that extracts in the fs bioreactor; The marine alga of in the fs bioreactor, cultivating like this will produce the higher XC polymer of lipid content in the subordinate phase bioreactor.
Patent principles such as the employing patent No. 4236349 are cultivated marine alga and existed weak point to be: the marine alga that the bioreactor of fs is cultivated is sent into the bioreactor of subordinate phase; Be through increasing bioreactor light-receiving area (changing structure of reactor or augmenting response device surface-area) and the light income way of (comprise and increase artificial lighting), making the marine alga of cultivation higher than the alginic cell inner lipid content of fs cultivation.Owing to get into the filtering separation of the algae liquid filtered device of subordinate phase bioreactor; Marine alga density is identical with the bioreactor of fs with the contained nitrogen concentration of algae liquid in the algae liquid; Though increased the light income of the unit surface of subordinate phase bioreactor; The marine alga speed of growth is accelerated, but nitrogenous source needs long time course in the marine alga consumption algae liquid, has therefore reduced the efficient and the production efficiency of alginic cell lipid conversion.
Present conventional way can only adopt the concentration of nitrogen element in the control bioreactor nutrient solution; Balanced growth and the demand that transforms two aspects; Consequently marine alga all can not reach mxm. at the protein contnt and the lipid content of transformation stage of growth phase, has had a strong impact on output and quality.In addition, need in same bioreactor pipeline, regulate the nitrogen element balance concentration in the marine alga nutrient solution, the difficulty of its control techniques difficulty and actually operating technology is also very high, is difficult to the realization in suitability for industrialized production.
Summary of the invention
The object of the present invention is to provide a kind of algae growth system device with two-step photobiologic reactors; The marine alga of cultivation is grown in the reactor drum of two kinds of different algae liquid respectively; The marine alga that the bioreactor of fs is cultivated is in containing the high concentration of nitrogen source; The protein contnt accumulation that is exceedingly fast improves the output of marine alga; Subordinate phase is through the filtering separation of strainer; Make marine alga break away from the former algae liquid that contains the nitrogen source high density; The entering lipid content carries out the process of height conversion, and raising obtains to be rich in the efficient of lipid high yield marine alga rapidly, thereby obtains the higher XC polymer of lipid content.
The said algae growth system device with two-step photobiologic reactors of the present invention, form by primary light bio-reactor A1, secondary light bio-reactor A2, algae liquid/gas separator A3 and detection system A4:
Primary light bio-reactor A1 is made up of one or above transparent tube 1, closed head 2, pipe connecting 3, dioxide gas input channel 4, mixed algae liquid input channel 5, PH regulator solution input channel 6, algae liquid recycle pump 7, pipe blow-through 8,9, vent valve 10 and algae liquid discharge line 11;
Secondary light bio-reactor A2 is made up of one or above transparent tube 12, closed head 13, pipe connecting 14, dioxide gas input channel 15, pregnant solution input channel 16, PH regulator solution input channel 17, algae liquid recycle pump 18, pipe blow-through 19,20, vent valve 21, pregnant solution transport pipe 26 and pregnant solution transferpump 27;
Algae liquid transport pipe 24, marine alga liquid transport pipe 25 were formed after algae liquid/gas separator A3 was filtered by liquid pump 22, strainer 23, strainer;
Detecting system A4 is made up of mixed algae liquid storage tank 28, dioxide gas storage tank 29, PH regulator solution storage tank 30, pregnant solution storage tank 31 and detection control apparatus 32;
Wherein between said primary light bio-reactor A1 and secondary light bio-reactor A2, be connected to a cover algae liquid/gas separator A3, algae liquid/gas separator A3 is made up of liquor pump 22 and strainer 23.Strainer is installed in the exit of strainer 23 filters back algae liquid transport pipe 24 and marine alga liquid transport pipe 25; Strainer filters back algae liquid transport pipe 24 and is connected with mixed algae liquid basin 28, and marine alga liquid transport pipe 25 is connected with the closed head 13 of secondary light bio-reactor A2.
Adopt the algae growth system device with two-step photobiologic reactors of the said structure of the present invention; In the fs of cultivating marine alga; Use can promote the marine alga growth nutrient solution of growth fast; Accelerate the speed of marine alga growth, make marine alga be exponential form, improved the content of seaweed biomass output and intracellular protein and glucide in the growth of primary light bio-reactor A1; Before the marine alga that fs primary light bio-reactor A1 is reached results density is sent into the secondary light bio-reactor A2 of subordinate phase; Increase an algae liquid/gas separator A3; With most of original growth nutrient solution in the strainer 23 first elimination algae liquid; Again marine alga is after filtering sent among the secondary light bio-reactor A2 of subordinate phase; It is different with the used nutrient solution of fs primary light bio-reactor A1 in subordinate phase secondary light bio-reactor A2, to cultivate the employed nutrient solution of marine alga, and being does not have nitrogenous source can accelerate the lipid-rich nutrient solution that alginic cell internal protein and glucide are converted into lipid.The marine alga that gets into subordinate phase secondary light bio-reactor A2 is under the effect of sunlight and pregnant solution; Consume the nitrogenous source in the algae liquid rapidly; Under the growth conditions that nitrogen lacks, marine alga just can be accelerated the speed that material transforms in the alginic cell and improved the content that lipid transforms.
In the fs of marine alga growth; Need not consider that nitrogen concentration in the algae liquid or other material element can exert an influence to the subordinate phase that the marine alga lipid transforms, therefore can use the growth nutrient solution that promotes the marine alga growth and improve Sargassum protein matter and carbohydrate content.Through the algae liquid/gas separator, the contained growth nutrient solution of marine alga of entering subordinate phase secondary light bio-reactor seldom shortens the time course of nitrogenous source in the marine alga consumption algae liquid greatly.Simultaneously, do not have the pregnant solution of nitrogenous source can improve the lipid transformation efficiency and the conversion rate of alginic cell internal protein and glucide again, thereby in the unit time, improved seaweed biomass output and lipid content.
Adopt the algae growth system device with two-step photobiologic reactors of the said structure of the present invention; Because primary light bio-reactor A1 and secondary light bio-reactor A2 are relatively independent; Can avoid the contradiction of growth of marine alga in the bioreactor and lipid-rich effectively; Help using respectively the special culture solution that can promote marine alga growth or lipid enrichment, in different bioreactors, can realize that the marine alga of rich in proteins and glucide and the marine alga output that is rich in lipid reach maximum respectively.
Bioreactor marine alga growth system device unit is the unit of the most basic production marine alga; Can be arbitrarily made with needed module number; Form a growing system module with a plurality of growing systems unit symmetrical or that be parallel to each other; To reach the industrial scale of marine alga output according to the marine alga output of each growing system module, marine alga oil length and expection, can confirm that growing system needs the total quantity of module, and control easily.Be applicable to the mode of the suitability for industrialized production marine alga of any scale, and need do not do any change bioreactor marine alga growth system device unit.Because the marine alga growth was isolated mutually with lipid two different stages of conversion; Can carry out independent regulation and control to the employed nutrient solution of bioreactor respectively; The marine alga that makes rich in proteins and glucide reaches peak respectively with the marine alga output that is rich in lipid, thereby avoids the contradiction of marine alga growth and lipid-rich in the bioreactor effectively.In different bioreactors, not only can make the marine alga ramp; Be exceedingly fast and improve the interior high protein of alginic cell and the content of glucide; And can accelerate the speed that transforms to lipid and improve transformation efficiency; Thereby obtain enough needed lipids of scale operation biofuel, realize industrialized high yield and continuous production.
Description of drawings
Fig. 1 is the connection synoptic diagram of the said algae growth system device with two-step photobiologic reactors of the present invention;
Fig. 2 is the growing system unit combination synoptic diagram of the said algae growth system device with two-step photobiologic reactors of the present invention;
Fig. 3 is the growing system module diagram that 10 growing system unit of the said algae growth system device with two-step photobiologic reactors of the present invention are formed.
Embodiment
The structure annexation and the mode of production below in conjunction with the said algae growth system device with two-step photobiologic reactors of detailed description of the drawings the present invention:
As shown in Figure 1, the said algae growth system device with two-step photobiologic reactors of the present invention is made up of primary light bio-reactor A1, secondary light bio-reactor A2, algae liquid/gas separator A3 and detection system A4:
Primary light bio-reactor A1 is made up of one or above transparent tube 1, closed head 2, pipe connecting 3, dioxide gas input channel 4, mixed algae liquid input channel 5, PH regulator solution input channel 6, algae liquid recycle pump 7, pipe blow-through 8,9, vent valve 10 and algae liquid discharge line 11;
Secondary light bio-reactor A2 is made up of one or above transparent tube 12, closed head 13, pipe connecting 14, dioxide gas input channel 15, pregnant solution input channel 16, PH regulator solution input channel 17, algae liquid recycle pump 18, pipe blow-through 19,20, vent valve 21, pregnant solution transport pipe 26 and pregnant solution transferpump 27;
Algae liquid transport pipe 24, marine alga liquid transport pipe 25 were formed after algae liquid/gas separator A3 was filtered by liquid pump 22, strainer 23, strainer;
Detecting system A4 is made up of mixed algae liquid storage tank 28, dioxide gas storage tank 29, PH regulator solution storage tank 30, pregnant solution storage tank 31 and detection control apparatus 32;
Wherein between said primary light bio-reactor A1 and secondary light bio-reactor A2, be connected to a cover algae liquid/gas separator A3; Algae liquid/gas separator A3 is made up of liquor pump 22 and strainer 23; Strainer is installed in the exit of strainer 23 filters back algae liquid transport pipe 24 and marine alga liquid transport pipe 25; Strainer filters back algae liquid transport pipe 24 and is connected with mixed algae liquid basin 28, and marine alga liquid transport pipe 25 is connected with the closed head 13 of secondary light bio-reactor A2.
Among the figure: A1-primary light bio-reactor A2-secondary light bio-reactor
A3-algae liquid/gas separator A4-detects system
A5-algae liquid means for breaking walls
1-primary reactor transparent pipeline 2-closed head
3-pipe connecting 4-carbonic acid gas input channel
5-mixed algae liquid input channel 6-PH regulator solution input channel
7-algae liquid recycle liquid pump 8-pipe blow-through
9-pipe blow-through 10-snifting valve
11-algae liquid discharge line 12-primary reactor transparent pipeline
13-closed head 14-pipe connecting
15-carbonic acid gas input channel 16-pregnant solution input channel
17-PH regulator solution input channel 18-algae liquid recycle liquid pump
19-pipe blow-through 20-pipe blow-through
21-snifting valve 22-liquid pump
23-strainer 24-strainer filters back algae liquid input channel
25-marine alga liquid transport pipe 26-pregnant solution transport pipe
27-pregnant solution transferpump 28-mixed algae liquid storage tank
29-carbon dioxide storage tank 30-PH regulator solution storage tank
31-pregnant solution storage tank 32-detection control apparatus
As shown in Figure 2; The unitary connection synoptic diagram of a growing system of the said algae growth system device with two-step photobiologic reactors of the present invention detects system 32 and controls primary light bio-reactor A1, secondary light bio-reactor A2, algae liquid/gas separator A3, mixed algae liquid storage tank 28, dioxide gas storage tank 29, PH regulator solution storage tank 30 and pregnant solution storage tank 31 simultaneously.Wherein: the mixed algae liquid of mixed algae liquid storage tank device 28 is through mixed algae liquid input channel 5 mixed algae liquid to be directly inputted in the primary light bio-reactor A1; The pregnant solution of pregnant solution tank arrangement 31 is through pregnant solution input channel 16 pregnant solution to be sent in the secondary light bio-reactor A2; The dioxide gas of dioxide gas storage tank 29 is to send in primary light bio-reactor A1 and the secondary light bio-reactor A2 through input channel 4,15 respectively; The PH regulator solution of PH regulator solution tank arrangement 30 is sent in primary light bio-reactor A1 and the secondary light bio-reactor A2 through input channel 6,17 respectively; The algae liquid of primary reactor A1 is imported among the secondary reactor A2 through marine alga liquid transport pipe 25 after algae liquid/gas separator A3 filters; Algae liquid after strainer 23 filters algae liquid input channel 24 after strainer filters carries back-mixing to close liquid storage tank 28, detects 32 pairs of unitary working ordeies of growing system of system and detects and control.
As shown in Figure 3; In the actual production place; The said algae growth system device with two-step photobiologic reactors of the present invention can be made up of the growing system unit more than 2 groups, provides the growing system module diagram of being made up of 10 growing system unit here.In practical large-scale is produced; In order to improve the light income of bioreactor; The preferably all orthogonal thereto layout of said primary light bio-reactor A1 and secondary light bio-reactor A2; And the quantity ratio of primary light bio-reactor A1 and the transparent tube 1,12 of secondary light bio-reactor A2 is preferably 2: 1, and all mixed algae liquid storage tanks 28, dioxide gas storage tank 29, PH regulator solution storage tank 30 and pregnant solution storage tank 31 can directly be access to through transmission pipeline and respectively organize in the growing system unit.
The said a kind of algae growth system device with two-step photobiologic reactors of the present invention; Can be used for the cultured continuously of any suitability for industrialized production scale to marine alga; Not only can use and promote the marine alga growth nutrient solution of growth fast; Make marine alga ramp in primary light bio-reactor A1, improve the yield of biomass of marine alga, and promoting alginic cell internal protein and glucide high-level efficiency to be converted under the pregnant solution effect of lipid; Make the marine alga of secondary light bio-reactor A2 accelerate intracellular protein and be converted into the speed and raising lipid transformation efficiency of lipid, thereby can obtain to be rich in the seaweed biomass of lipid high yield with glucide.
The said two-step approach bioreactor of the present invention marine alga growing system is by forming with lower unit:
1, primary light bio-reactor
With the pipeline 1 of the identical transparent material making of diameter shown in Figure 1, and closed head 2; Pipe connecting 3; Liquid input tube road 5,6,8,9 and gas input channel 4; Algae liquid recycle pump 7; It is a primary light bio-reactor A1 that vent valve 10 is formed two U type pipelines perpendicular to the ground with algae liquid discharge line 11.
The mixed algae liquid that contains nutritive medium and marine alga in the mixed algae liquid storage tank 28 is pumped in the primary light bio-reactor A1 transparent pipeline 1 through pipeline 5, and the various parameters of marine alga growth detect and control in 32 pairs of reactor drums of detection control apparatus.According to the control needs; Import CO2 gas from CO2 storage tank 29 to primary light bio-reactor A1 transparent pipeline 1 through pipeline 4; Import PH regulator solution from PH regulator solution storage tank 30 to primary light bio-reactor A1 transparent pipeline 1 through pipeline 6, make the algae liquid in the primary light bio-reactor A1 transparent pipeline 1 keep best CO2 concentration and PH concentration.Under the effect of sunlight and nutritive medium, the marine alga speed of growth is exponential form.The effect of primary light bio-reactor A1 is the algae liquid that obtains high-density rich in proteins and glucide marine alga; When the marine alga of primary light bio-reactor A1 reaches results density; Quantitatively take out a certain amount of marine alga liquid from primary light bio-reactor A1 at every turn, pump into the mixed algae liquid of equivalent simultaneously.
2, algae liquid/gas separator
Form by liquid pump 22, strainer 23, strainer filtration back algae liquid transport pipe 24 and marine alga liquid transport pipe 25 with algae liquid/gas separator A3 shown in Figure 1; Strainer can adopt various forms of strainers; Strainer major function: through algae liquid discharge line 11; Liquid pump 22 is transported to strainer 23 with a certain amount of algae liquid in the primary light bio-reactor A1; 23 pairs of algae liquid of strainer filter, and algae liquid is after filtering sent mixed algae liquid storage tank 28 back to through pipeline 24, and the marine alga that obtains is after filtering sent into secondary light bio-reactor A2 through marine alga liquid transport pipe 25.Adopt full-automatic filter, can not use liquid pump.
3, secondary light bio-reactor
The pipeline made from the transparent material identical shown in Figure 1 12, and closed head 13 with primary light bio-reactor A1 transparent pipeline 1 diameter; Pipe connecting 14; Liquid input tube road 16,17,19,20 and gas input channel 15; Algae liquid recycle pump 18; The U type transparent pipeline that vent valve 21, enrichment algae liquid transport pipe 26 and enrichment algae liquid transferpump 27 compositions and ground level are placed is as a secondary light bio-reactor A2.
When the marine alga density in the elementary bioreactor A1 transparent pipeline 1 reaches results density; Algae liquid with some amount in the primary light bio-reactor A1 transparent pipeline 1; Liquid pump 22 through algae liquid/gas separator A3 is sent into algae liquid filter 23; Most of algae liquid is removed in strainer filter 23; Marine alga after filtering is sent in the secondary light bio-reactor A2 transparent pipeline 12 through marine alga liquid transport pipe 25, in secondary light bio-reactor A2 transparent pipeline 12, pumped into the pregnant solution that promotes the marine alga lipid to transform nonnitrogenous source from pregnant solution storage tank 31 through pipeline 16 simultaneously.Detection control apparatus 32 is according to the control needs; Import PH regulator solution from PH regulator solution storage tank 30 to secondary light bio-reactor A2 transparent pipeline 12 through pipeline 17; Import CO2 gases from CO2 storage tank 29 to secondary light bio-reactor A2 transparent pipeline 12 through pipeline 15, make the algae liquid in the secondary light bio-reactor A2 transparent pipeline 12 keep best PH concentration and CO2 concentration.Transparent pipeline 12 parallel lay-ups of secondary light bio-reactor A2, light receiving surface is long-pending greater than primary light bio-reactor A1.Therefore, the marine alga speed of growth of secondary light bio-reactor A2 is faster than primary light bio-reactor A1's, can accelerate to consume the nitrogenous source in the algae liquid.Under the effect of sunlight and pregnant solution, the lipid that pregnant solution impels marine alga to accelerate intracellular protein and glucide transforms, and coerces alginic cell and reaches higher oleaginousness (comparing with the marine alga of primary light bio-reactor A1).When primary light bio-reactor A1 algae liquid reaches results density once more; Algae liquid with secondary light bio-reactor A2 is transported to algae liquid means for breaking walls A5 through enrichment algae liquid transport pipe 26 and enrichment algae liquid transferpump 27 earlier; Again the algae liquid pump of primary light bio-reactor A1 is gone into algae liquid filter 23, set up the continuous production of marine alga with this.Adopt different algae kind, nutritive medium and pregnant solution, seaweed biomass output, the speed that is converted into lipid and transformation efficiency are inequality.
Vent valve 10 is installed on the closed head of primary light bio-reactor A1, secondary light bio-reactor A2, gas in the automatic discharge tube of ability when gas reaches setup pressure value in the reactor drum transparent pipeline.
4, detect system
Form by mixed algae liquid storage tank 28, CO2 storage tank 29, PH regulator solution storage tank 30, pregnant solution storage tank 31, detection control apparatus 32 with detection system A4 shown in Figure 1.
4.1 detection control apparatus 32 is according to the detected result to the various growth parameter(s)s of marine alga; Process of growth to marine alga in the reactor drum is controlled; According to the needs of cultivating marine alga; With the mixed algae liquid of mixed algae liquid storage tank 28, the CO2 of CO2 storage tank 29, the PH regulator solution of PH regulator solution storage tank 30, the pregnant solution of pregnant solution storage tank 31, send into primary light bio-reactor A1 of unit and secondary light bio-reactor A2 respectively through pipeline, and control best input automatically.
4.2 the algae liquid recycle liquid pump 7 and 18 that bioreactor is outside; Respectively with primary light bio-reactor and secondary light bio-reactor U type pipe ends closed head on pipeline interconnect; Algae liquid in the bioreactor transparent pipeline flows at bioreactor pipeline internal recycle under the effect of algae liquid recycle liquid pump.
4.3 detection control apparatus at different growth phases, to the parameter of CO2 requirement, is controlled the CO2 input according to algae liquid in the bioreactor transparent pipeline automatically.CO2 gas is followed algae liquid input reactor transparent pipeline and is formed micro-bubble, in the moving process of algae flow, fully realizes gaseous interchange with marine alga, effectively replenishes the CO2 that alginic cell photosynthesis consumes.Because the O2 molecular weight is 32; The CO2 molecular weight is 44; Alginic cell photosynthesis is resolved the top that the O2 that discharges can accumulate in pipeline; For avoiding the oxidative damage effect of too high dissolved oxygen, after gaseous tension reaches set(ting)value in each U type pipeline, discharge automatically through vent valve 10 or 21 to frustule.
4.4 detection control apparatus according to the pH value optimum parameter of marine alga in the bioreactor at different growth phase needs, is controlled the input of pH value regulator solution automatically.In the pH value regulator solution input reactor transparent pipeline, in the moving process of algae flow, mix rapidly, spread, effectively regulate the pH value of algae liquid, make algae liquid keep the PH concentration of the most suitable marine alga growth.
4.5 marine alga growth parameter(s) detection control apparatus 32 comprises: TP, PH transmitter, algae liquid density sensor, CO2 transmitter, PO2 transmitter, gas pressure sensor, conductivity sensor etc., and with control marine alga various kinds of sensors and the gear that relevant parameter detecting uses of growing.Transmitter detects the various growth parameter(s)s of marine alga, and gear is controlled the growing system state automatically.
5, growing system
Primary light bio-reactor A1, algae liquid/gas separator A3, a secondary light bio-reactor A2 and detect system A4 and form a growing system unit, with symmetrically or a plurality of growing systems unit that is parallel to each other form a growing system module.To reach the industrial scale of marine alga output according to the marine alga output of each growing system module, marine alga oil length and expection, can confirm that growing system needs the total quantity of module.
6, the way that adopts increase primary light bio-reactor A1 and secondary light bio-reactor A2 to receive the optical transparency pipe diameter and prolong the pipeline total length realizes enlarging pipe reactor volume purpose.According to cultivating the different needs of marine alga output, the transparent pipeline diameter be 20cm to 40cm or more than, each U type transparent pipeline total length 5m to 100m or more than.
7, pipe blow-through 8,9 is connected with outside scavenging pump, utilizes the algae hydraulic pressure official post cleaning part at cleaning part two ends to produce motion, can clean forward or backwards transparent pipeline 1.Pipe blow-through 19,20 is connected with outside scavenging pump, utilizes the algae hydraulic pressure official post cleaning part at cleaning part two ends in the pipe to produce motion, can clean forward or backwards transparent pipeline 12.
8, bioreactor marine alga growth system device should be based upon the competent place of outdoor solar ray, makes full use of nature sunlight, and the photosynthesis that increases marine alga improves marine alga output, and the reduction scale is cultivated the cost of marine alga.
Adopt the principle of work of the algae growth system device with two-step photobiologic reactors of the said structure of the present invention:
Through pipeline 5 mixed algae liquid is sent into respectively in the primary light bio-reactor A1 transparent pipeline 1, under the effect of recycle pump 7, algae liquid is answering organ pipe road internal recycle to flow.CO2 gas is through pipeline 4 input reactors, and the PH regulator solution is through pipeline 6 input reactors, and marine alga is growth fast in bioreactor.When the marine alga of growth reactor reaches results density; Liquid pump 22 is sent a part of marine alga liquid of primary light bio-reactor A1 into strainer 23 and is filtered; The marine alga that obtains after the filtration is sent in the secondary light bio-reactor A2 transparent pipeline 12 through pipeline 25; Liquid pump arrives primary light bio-reactor A1 through the mixed algae liquid that pipeline 5 pumps into equivalent simultaneously, and the algae liquid after strainer 23 filters is sent mixed solution storage tank 28 back to through pipeline 24.
Send into the pregnant solution that promotes that the marine alga lipid transforms to secondary light bio-reactor A2 transparent pipeline 12 through pipeline 16; CO2 gas is through pipeline 15 input reactors; The PH regulator solution is through pipeline 17 input reactors; Marine alga in the transparent pipeline 12 is accelerated lipid and transforms under sunlight and pregnant solution effect; Marine alga reaches the oil length of expection, and liquid pump 27 is delivered to algae liquid means for breaking walls A5 with the algae liquid in the secondary light bio-reactor A1 transparent pipeline 12 through pipeline 26, and liquid pump 22 is sent the algae liquid that reaches results density in the primary light bio-reactor A1 transparent pipeline 1 into strainer 24 again; Marine alga after strainer filters is sent in the secondary light bio-reactor A2 transparent pipeline 12, circulates with this.
Through algae liquid/gas separator A3; The marine alga of sending into secondary light bio-reactor A2 is by the most of growth nutrient solution of elimination; Since marine alga in the secondary light bio-reactor A2 speed of growth greater than primary light bio-reactor A1; Under the situation of not using efficiently concentrating liquid, reduce nutrient solution nitrogen concentration (comparing) with growth nutrient solution, adopt two-step approach still can obtain higher being rich in lipid marine alga output and can realizing continuous production than single reaction vessel.
The marine alga that the present invention cultivates comprises all algae in the environment such as coming from fresh water, salt water and seawater, as: other algae such as flat algae, chlorella, blue-green algae, green alga, grid algae, haematococcus pulvialis, Porphyridium cruentum, snow algae or diatom.The algae of cultivating can be used for producing hydrocarbon polymer, glucide, protein and high value element product, and is used for aquaculture bait and carries out special bioactive ingredients exploitation.
The transparent pipeline of bioreactor of the present invention adopts the light transmissive material that comprises any thickness of pipe, color and luster, quality, as: glass and plastics (polymethylmethacrylate (PMMA), PS (PS), polycarbonate (PC), styrene-acrylonitrile (AS), styrene-methylmethacrylate copolymer (MS), poly(4-methyl-1-pentene) (TPX), gather-4-methyl-1-pentene (PMP) and transparent polyamide (PA) etc.).The transparent pipeline diameter from 20cm to 40cm or more than, each U type transparent pipeline total length from 5m to 100m or more than.
Material of the present invention comprises respectively: water; Algae liquid; CO2 gas; The nutritive substance that inorganics and humatite matter are formed, the regulator solution that algae liquid pH value is regulated and other help nutritive medium that marine alga growth and lipid transform and growth liquid etc.
Algae growth system device with two-step photobiologic reactors of the present invention can be the growing system module that the growing system unit more than 2 is formed, and the growing system module more than 2 can be formed needed growing system.To reach the industrial scale of marine alga output according to the marine alga output of each growing system module, marine alga oil length and expection, can confirm that bioreactor marine alga growing system needs the total quantity of module.
The algae growth system device with two-step photobiologic reactors of the said structure of the present invention; The pipeline of primary light bio-reactor A1 adopts vertical placement of U type transparent pipeline to compare with U type transparent pipeline horizontal positioned; U type pipe is vertically placed: under the identical situation of land area; Number of tubes, light application time and the light-receiving area of U type pipe upper parallel is identical with the U type pipe of horizontal positioned; Though U type pipe lower pipeline can receive the influence of upper pipe shade, and certain growth efficiency is also arranged, bioreactor volume and the efficient of producing marine alga have been improved.
The algae growth system device with two-step photobiologic reactors of the said structure of the present invention; Utilize the outside liquid pump of bioreactor, make the bioreactor marine alga growth system device that the generation of algae liquid circulates in the reactor drum, because reactor drum transparent pipeline inside does not have obstacle; Relatively independent between each bioreactor marine alga growth system device unit; Can adopt cleaning head that inwall is cleaned, washing unit is simple in structure, and failure rate is low.
Utilize the production technology of closed photo bioreactor large scale culturing marine alga; It is the Development Trend of seaweed bio technology; Algae growth system device with two-step photobiologic reactors is that design realizes continuous production and obtains to be rich in a kind of effective industrial installation of lipid high yield marine alga.
Claims (2)
1. algae growth system device with two-step photobiologic reactors, by primary light bio-reactor (A1), secondary light bio-reactor (A2), algae liquid/gas separator (A3) with detect system (A4) and form:
Primary light bio-reactor (A1) is made up of transparent tube (1), closed head (2), pipe connecting (3), dioxide gas input channel (4), mixed algae liquid input channel (5), PH regulator solution input channel (6), algae liquid recycle pump (7), two pipe blow-throughs (8,9), vent valve (10) and algae liquid discharge line (11);
Secondary light bio-reactor (A2) is made up of transparent tube (12), closed head (13), pipe connecting (14), dioxide gas input channel (15), pregnant solution input channel (16), PH regulator solution input channel (17), algae liquid recycle pump (18), two pipe blow-throughs (19,20), vent valve (21), pregnant solution transport pipe (26) and pregnant solution transferpump (27);
Algae liquid transport pipe (24), marine alga liquid transport pipe (25) were formed after algae liquid/gas separator (A3) was filtered by liquor pump (22), strainer (23), strainer;
Detecting system (A4) is made up of mixed algae liquid storage tank (28), dioxide gas storage tank (29), PH regulator solution storage tank (30), pregnant solution storage tank (31) and detection control apparatus (32);
It is characterized in that: be connected to a cover algae liquid/gas separator (A3) between primary light bio-reactor (A1) and the secondary light bio-reactor (A2); Algae liquid/gas separator (A3) is made up of liquor pump (22) and strainer (23); Strainer is installed in the exit of strainer (23) filters back algae liquid transport pipe (24) and marine alga liquid transport pipe (25); Strainer filters back algae liquid transport pipe (24) and is connected with mixed algae liquid basin (28), and marine alga liquid transport pipe (25) is connected with the closed head (13) of secondary light bio-reactor (A2).
2. according to the said two-step approach bioreactor of claim 1 marine alga growing apparatus, it is characterized in that the quantity of said primary light bio-reactor (A1) transparent tube (1): the quantity of the transparent tube (12) of secondary light bio-reactor (A2) is 2: 1.
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| US20120156669A1 (en) | 2010-05-20 | 2012-06-21 | Pond Biofuels Inc. | Biomass Production |
| US11512278B2 (en) | 2010-05-20 | 2022-11-29 | Pond Technologies Inc. | Biomass production |
| AU2011256085A1 (en) * | 2010-05-20 | 2013-01-10 | Pond Biofuels Inc. | Biomass production |
| CN103627620A (en) * | 2012-08-28 | 2014-03-12 | 西门子公司 | Method and apparatus for producing lipids through microalgae culture |
| CN105254020B (en) * | 2015-11-17 | 2018-04-03 | 江西师范大学 | Method for quickly separating algae liquid in wastewater of microalgae treatment |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4087936A (en) * | 1976-12-13 | 1978-05-09 | Mobil Oil Corporation | Process for production of alga biopolymer and biomass |
| CN1096052A (en) * | 1993-05-31 | 1994-12-07 | 国家研究发展公司 | A kind of method of producing exsiccant algae bio amount from Spirullina |
| CN101280328A (en) * | 2008-05-27 | 2008-10-08 | 清华大学 | Method for producing biodiesel by autotrophic culture and heterotrophic culture of chlorella |
| WO2009105733A2 (en) * | 2008-02-23 | 2009-08-27 | James Weifu Lee | Designer organisms for photobiological butanol production from carbon dioxide and water |
-
2009
- 2009-08-28 CN CN 200910042208 patent/CN101643700B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4087936A (en) * | 1976-12-13 | 1978-05-09 | Mobil Oil Corporation | Process for production of alga biopolymer and biomass |
| CN1096052A (en) * | 1993-05-31 | 1994-12-07 | 国家研究发展公司 | A kind of method of producing exsiccant algae bio amount from Spirullina |
| WO2009105733A2 (en) * | 2008-02-23 | 2009-08-27 | James Weifu Lee | Designer organisms for photobiological butanol production from carbon dioxide and water |
| CN101280328A (en) * | 2008-05-27 | 2008-10-08 | 清华大学 | Method for producing biodiesel by autotrophic culture and heterotrophic culture of chlorella |
Non-Patent Citations (1)
| Title |
|---|
| 黄冠华等.两步培养法提高蛋白核小球藻的油脂含量.《华南理工大学学报(自然科学版)》.2008,第36卷(第12期),97-101. * |
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