CN105543079B - A kind of tower photosynthetic bacteria continuously produces hydrogen reaction system and its production hydrogen methods - Google Patents
A kind of tower photosynthetic bacteria continuously produces hydrogen reaction system and its production hydrogen methods Download PDFInfo
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Abstract
The invention discloses a kind of tower photosynthetic bacterias continuously to produce hydrogen reaction system and its production hydrogen methods, it is therefore intended that solves the problems, such as that illuminance attenuation is excessive caused by hydrogen-manufacturing reactor volume is big in the prior art, temperature difference is big, produces that hydrogen process conditions are inaccurate, hydrogen generation efficiency is low.System includes material feeding box, feed pump, several hollow board-like production hydrogen reactors, support column, LED light strip, gas-liquid separating box, expansion box and waste liquid collecting box.It is system reasonable design, simple and practicable the present invention relates to the technical field of biomass photosynthetic-hydrogen-production, it can realize that illuminance and temperature are consistent with requirement of experiment value inside hydrogen-manufacturing reactor, are improved and are produced hydrogen process conditions accuracy and hydrogen generation efficiency.
Description
Technical field
The present invention relates to the technical fields of biomass photosynthetic-hydrogen-production, and in particular to it is anti-that a kind of tower photosynthetic bacteria continuously produces hydrogen
Answer system and production hydrogen methods.
Background technology
The energy is the motive power for industrializing high speed development, is that the central factor of social economy's fast development and strategy is supported to provide
Source.With the swift and violent growth of social resources demand, available non-renewable resources are fewer and fewer on the earth, and environment is also more next
It is poorer, so as to develop the new energy ease up solution environmental pollution become it is instantly social in the urgent need to address the problem of.Hydrogen Energy is because of its energy
Metric density height such as safety and environmental protection, is readily transported, is renewable at many characteristics, is known as " following energy ".
The market of China's hydrogen manufacturing at present is almost monopolized by the hydrogen manufacturing of fossil fuel physical-chemical process and water electrolysis method hydrogen manufacturing, biology system
Hydrogen is still in the starting stage.The research of biological hydrogen production focuses mostly on excellent in pretreatment of raw material, high-efficiency strain enrichment, production hydrogen process conditions
Change and the design of production hydrogen reactor etc..Illumination is in hydrogen production with photosynthetic bacteria reaction solution as transmission distance increase attenuation is existing
As apparent, temperature also can be increasing with experimental setup temperature difference with the increase of hydrogen production bioreactor in reaction solution, this is just limited
The popularization of production with photosynthetic bacteria hydrogen production device, and then the serious large-scale development for constraining photosynthetic-hydrogen-production are made.
Invention content
In view of the deficiencies of the prior art, the present invention is intended to provide a kind of tower photosynthetic bacteria continuously produce hydrogen reaction system and its
Hydrogen methods are produced, by using multiple hollow board-like production hydrogen reactors, and biomass straw enzymolysis liquid and photosynthetic bacteria are made to mix shape
Into reaction solution flow circuit successively from bottom to top so that the hydrogen generated in every layer of production hydrogen reactor drives reaction solution flowing,
Concentration, temperature in each production hydrogen reactor is uniform；And lighting device is configured in each production hydrogen reactor, and it is anti-to be responsible for each production hydrogen
The illumination of the production hydrogen in device is answered, avoids illumination in hydrogen production with photosynthetic bacteria reaction solution as transmission distance increase relaxation phenomenon is apparent
The problem of.
To achieve these goals, the present invention adopts the following technical scheme that：
A kind of tower photosynthetic bacteria continuously produces hydrogen reaction system, the hollow board-like production hydrogen reactor including multiple light transmissions；Institute
There is production hydrogen reactor to be vertically corresponding in turn to arrangement in tower-like；Pass through center tube phase between the adjacent production hydrogen reactor of each two
Connection, the top of center tube are communicated in the bottom centre position of production hydrogen reactor being located above, and end, which is then communicated in, to be located at
The top center of the production hydrogen reactor of lower section；The top surface of each production hydrogen reactor is equipped with lighting device；Positioned at the bottom
Production hydrogen reactor bottom is communicated at least one feed pump, and the feed pump is then communicated at least one material feeding box；Positioned at most
At least one gas-liquid separating box is communicated at the top of the production hydrogen reactor of top layer, is communicated at least at the top of the gas-liquid separating box
One expansion box, and the side of gas-liquid separating box is then communicated in waste liquid collecting box.
Further, the lighting device each set by production hydrogen reactor top surface includes using center tube as a system in the center of circle
Row concentric circles LED light strip.
Further, the LED light strip is in equidistant concentric arrays.
Further, the bottom surface of the production hydrogen reactor is circular flat, and top surface then gradually increases oblique for ecto-entad
Face.
Further, the production hydrogen reactor is mainly made of organic glass.
Further, the feed pump is constant flow pump.
Further, at least two support columns are further included, each hydrogen reactor that produces is all connected to the support column.
The production hydrogen methods that hydrogen reaction system is continuously produced using above-mentioned tower photosynthetic bacteria are included the following steps：
S1 adds in photosynthetic bacteria and biomass straw enzymolysis liquid in material feeding box by a certain percentage, is mixed to get reaction solution,
Then power on, then feed pump and LED light strip are opened；Biomass straw enzymolysis liquid is to utilize cellulase by biomass straw
The supernatant obtained after being digested, wherein containing reduced sugar；
Reaction solution in S2 material feeding box initially enters the production hydrogen reactor positioned at the bottom under the action of feed pump, and from
Under up flow and full of each production hydrogen reactor, each photosynthetic bacteria produced in hydrogen reactor in reaction solution is anti-in accordingly production hydrogen
The illumination of the LED light strip set by device is answered biomass straw enzymolysis liquid to be utilized to carry out photosynthetic hydrogen production under acting on；
S3 each produces the hydrogen generated in hydrogen reactor under buoyancy, from outer lateral intermediate flow, and from lower part to
Upper flow is moved with the movement of upper strata reaction solution ecto-entad, lower floor's reaction solution in movable property hydrogen reactor, from inside to outside so as to real
Now produce self stirring of reaction solution in hydrogen reactor；
The hydrogen generated in each production hydrogen reactors of S4 produces hydrogen reactor simultaneously by each from bottom to top under buoyancy
Enter expansion box into gas-liquid separating box, and by gas-liquid separating box；Reaction solution also under the action of the feed pump after pass through by
Gas-liquid separating box is entered, and waste liquid collecting box is flowed into from gas-liquid separating box side positioned at the production hydrogen reactor of top.
Further, the photosynthetic bacteria that step S1 is added in, to be inoculated with the photosynthetic bacteria in logarithmic phase later stage, with
30% bacterial load and 70% growth medium are cultivated under the conditions of intensity of illumination is 3000lx, temperature is 30 DEG C
It obtains.
Further, in step S1, the feed pump uses constant flow pump, by adjusting the flow velocity of feed pump, makes system
Hydraulic retention time is 30-36h so that the photosynthetic bacteria in growth period is continuously replenished, discharges the photosynthetic bacteria of decline, it is ensured that
It produces photosynthetic bacteria in hydrogen reactor and keeps best physiological status.
The beneficial effects of the present invention are：
1st, it is independent using multiple hollow board-like production hydrogen reactors and from top to bottom hierarchal arrangement, each production hydrogen reactor progress
Produce hydrogen so that while hydrogen output is maintained, it is uneven to avoid the large-scale internal temperature produced present in hydrogen reactor, illuminance
The problem of；
2nd, by the setting and connection of material feeding box, feed pump, production hydrogen reactor and gas-liquid separating box, waste liquid collecting box etc.,
Realize continuous production hydrogen；
3rd, a lighting device is configured for each production hydrogen reactor, avoids in large-scale production hydrogen reactor illumination photosynthetic
As transmission distance increases the problem of relaxation phenomenon is apparent in Hydrogen Production by Bacteria reaction solution；
The 4th, lighting device is further designed as to a series of concentric circles LED light strip using center tube as the center of circle so that production
Hydrogen reactor bilateral illumination, lighting are uniform；
5th, hydrogen ecto-entad under buoyancy, floats from bottom to top, drives upper strata in hollow board-like production hydrogen reactor
Reaction solution ecto-entad moves, and lower floor's reaction solution moves from inside to outside, self is stirred so as to fulfill reaction solution in production hydrogen reactor,
So that photosynthetic bacteria and biomass straw enzymolysis liquid are sufficiently mixed, it ensure that reacting liquid temperature is uniform in plate-type reactor, concentration
Unanimously, it promotes biomass straw enzymolysis liquid and photosynthetic bacteria comes into full contact with；Meanwhile reaction efficiency is improved, it is not provided with stirring
Device reduces energy consumption；
6th, by being further used as feed pump using constant flow pump so that device water can be made by adjusting constant current flow rate pump
The power residence time is 30-36h, and the photosynthetic bacteria in growth period is continuously replenished, discharges the photosynthetic bacteria of decline, it is ensured that reactor
Middle photosynthetic bacteria keeps best physiological status；
7th, production hydrogen reactor center and extraneous distance are shortened, production hydrogen reactor surface area is increased, accelerates
Heat is transmitted, so as to which production hydrogen reactor internal temperature be made preferably to be consistent with ambient temperature, it is ensured that temperature in production hydrogen reactor
It is consistent with experimental design value, improve experiment accuracy；
8th, simple in structure, easy to operate, adaptability to raw material is strong, available for feces of livestock and poultry, agricultural crop straw, industrial and agricultural wastewater
The like waste recycling recycles.
Description of the drawings
Fig. 1 is the overall structure diagram of the embodiment of the present invention；
Fig. 2 is the overlooking the structure diagram of the production hydrogen reactor in Fig. 1；
Fig. 3 is the front schematic cross-section of the production hydrogen reactor in Fig. 1.
Specific embodiment
Below with reference to attached drawing, the invention will be further described, it should be noted that the present embodiment is with this technology side
Premised on case, detailed embodiment and specific operating process are given, but protection scope of the present invention is not limited to this reality
Apply example.
As shown in Figs. 1-3, a kind of tower photosynthetic bacteria continuously produces hydrogen reaction system, the hollow board-like production including multiple light transmissions
Hydrogen reactor, in the present embodiment, using four hollow board-like production hydrogen reactors.It is all to produce the hydrogen reactors vertically row of being corresponding in turn to
Row are in tower-like, are followed successively by the first production hydrogen reactor 7, second production hydrogen reactor 6,5 and of third production hydrogen reactor in Fig. 1 from lower to upper
4th production hydrogen reactor 4.
It is connected between the adjacent production hydrogen reactor of each two by center tube 13, the top of center tube 13 is communicated in
The bottom centre position of production hydrogen reactor being located above, end are then communicated in the top center of underlying production hydrogen reactor
Position.
The top surface of each production hydrogen reactor is equipped with lighting device 3.
Preferably, the lighting device 3 each set by production hydrogen reactor top surface includes with center tube 13 being the center of circle
A series of concentric circles LED light strip.Further, as a preferred embodiment, LED light strip is carried out in a manner of equidistant concentric circles
Arrangement.
In the present embodiment, as shown in Figure 2 and Figure 3, the LED light strip includes inside LED light strip 33 and outside LED light strip
32, diameter is respectively 125mm and 250mm, can continue to increase LED light strip, and straight with diameter 375mm, 500mm etc. as needed
Diameter is configured.In addition, interior outside LED light strip 33 and 32 can be supported, and pass through LED light strip power transmission line by fulcrum 31
8 are electrically connected.
Feed pump 10 is communicated in by woven hose positioned at the first production 7 bottom of hydrogen reactor of the bottom, and the feed pump
10 are communicated in material feeding box 11 by pipeline.Gas is communicated in by pipeline positioned at the top of the 4th production hydrogen reactor 4 of top
Liquid separator box 2, the top of the gas-liquid separating box 2 is communicated in expansion box 1 by appendix, and the side of gas-liquid separating box 2 is then
Waste liquid collecting box 12 is communicated in by pipeline.
As a kind of embodiment, production hydrogen reactor brim height is 100mm, there is the spacing of 10mm between production hydrogen reactor.
And center tube height is 120mm, a diameter of 40mm.
Preferably, the bottom surface of the production hydrogen reactor is circular flat, and top surface is then gradually increased for ecto-entad
Inclined-plane.As a kind of embodiment, the diameter of the circular flat is set as 1000mm.
Preferably, the production hydrogen reactor is mainly made of organic glass.
Preferably, the side of the gas-liquid separating box 2 is then communicated in the waste liquid collecting box 12 by pipeline
Top.
Preferably, the feed pump 10 is constant flow pump.
Preferably, support column 9 is further included, each hydrogen reactor that produces is all connected to the support column 9.
The production hydrogen methods that hydrogen reactor is continuously produced using above-mentioned tower photosynthetic bacteria are included the following steps：
S1 adds in photosynthetic bacteria and biomass straw enzymolysis liquid in material feeding box by a certain percentage, is mixed to get reaction solution,
Then power on, then feed pump and LED light strip are opened；Biomass straw enzymolysis liquid is by the straw utilization cellulose of biomass
The supernatant that enzyme obtains after being digested, wherein containing reduced sugar.Biomass straw can be by straw under the action of cellulase
Cellulose and hemicellulose in stalk are converted into reduced sugar, and photosynthetic bacteria can utilize reduction under the illumination effect of LED light strip
Sugar carries out photosynthetic hydrogen production.
Reaction solution in S2 material feeding box initially enters the production hydrogen reactor positioned at the bottom under the action of feed pump, and from
Under up flow and full of each production hydrogen reactor, each photosynthetic bacteria produced in hydrogen reactor in reaction solution is anti-in accordingly production hydrogen
The illumination of the LED light strip set by device is answered to act on the lower reduced sugar progress photosynthetic hydrogen production using in biomass straw enzymolysis liquid；
S3 each produces the hydrogen generated in hydrogen reactor under buoyancy, from outer lateral intermediate flow, and from lower part to
Upper flow is moved with the movement of upper strata reaction solution ecto-entad, lower floor's reaction solution in movable property hydrogen reactor, from inside to outside so as to real
Now produce self stirring of reaction solution in hydrogen reactor；
The hydrogen generated in each production hydrogen reactors of S4 produces hydrogen reactor simultaneously by each from bottom to top under buoyancy
Enter expansion box into gas-liquid separating box, and by gas-liquid separating box；Reaction solution also under the action of the feed pump after pass through by
Gas-liquid separating box is entered, and waste liquid collecting box is flowed to from gas-liquid separating box side positioned at the production hydrogen reactor of top.
Further, the photosynthetic bacteria that step S1 is added in, to be inoculated with the photosynthetic bacteria in logarithmic phase later stage, with
30% bacterial load and 70% growth medium are cultivated under the conditions of intensity of illumination is 3000lx, temperature is 30 DEG C
It obtains.
In the present embodiment, photosynthetic bacteria is using the Ministry of Agriculture of Agricultural University Of He'nan regenerative resource new material and equipment emphasis
The HAU-M1 photosynthetic hydrogen production bacteriums of field domestication that laboratory provides, identified, the strain is by Rhodospirillum rubrum
(Rhodospirillum rubrum) 27%, capsula Rhodopseudomonas (R.capsulata) 25%, Rhodopseudomonas palustris
(R.pulastris) 28%, hydrogenlike silicon ion (Rhodobacter sphaeroides) 9%, Rhodobacter capsulatus
(Rhodobacter capsulatus) 11% etc..
Further, in step S1, the feed pump uses constant flow pump, by adjusting the flow velocity of feed pump, makes system
Hydraulic retention time is 30-36h so that the photosynthetic bacteria in growth period is continuously replenished, discharges the photosynthetic bacteria of decline, it is ensured that
It produces photosynthetic bacteria in hydrogen reactor and keeps best physiological status.
For those skilled in the art, it can be made various corresponding according to above technical solution and design
Change and deform, and all these change and deformation should be construed as being included within the protection domain of the claims in the present invention.
Claims (9)
1. a kind of production hydrogen methods that hydrogen reaction system is continuously produced using tower photosynthetic bacteria, which is characterized in that described tower photosynthetic
Bacterium continuously produces the hollow board-like production hydrogen reactor that hydrogen reaction system includes multiple light transmissions；It is all to produce hydrogen reactors vertically successively
Corresponding arrangement is in tower-like；It is connected between the adjacent production hydrogen reactor of each two by center tube, the top of center tube connects
The bottom centre position of production hydrogen reactor being located above is passed through, end is then communicated in the top of underlying production hydrogen reactor
Center；The top surface of each production hydrogen reactor is equipped with lighting device；Positioned at the production hydrogen reactor bottom of the bottom be communicated in
A few feed pump, and the feed pump is then communicated at least one material feeding box；Positioned at the top of the production hydrogen reactor of top
At least one gas-liquid separating box is communicated in, is communicated at least one expansion box at the top of the gas-liquid separating box, and gas-liquid separation
The side of case is then communicated in waste liquid collecting box；
The production hydrogen methods include the following steps：
S1 adds in photosynthetic bacteria and biomass straw enzymolysis liquid in material feeding box by a certain percentage, is mixed to get reaction solution, then
Power on, then feed pump and LED light strip are opened；Biomass straw enzymolysis liquid is to be carried out biomass straw using cellulase
The supernatant obtained after enzymolysis, wherein containing reduced sugar；
Reaction solution in S2 material feeding box initially enters the production hydrogen reactor positioned at the bottom under the action of feed pump, and from lower past
It is upper to flow and full of each production hydrogen reactor, each produce the photosynthetic bacteria in hydrogen reactor in reaction solution in accordingly production hydrogen reactor
The illumination effect of set LED light strip is lower to carry out photosynthetic hydrogen production using the reduced sugar in biomass straw enzymolysis liquid；
S3 each produces the hydrogen generated in hydrogen reactor under buoyancy, from outer lateral intermediate flow, and from lower to upper part
Flowing, with reaction solution ecto-entad movement in upper strata in movable property hydrogen reactor, lower floor's reaction solution moves from inside to outside, so as to fulfill production
Self stirring of reaction solution in hydrogen reactor；
The hydrogen generated in each production hydrogen reactors of S4 under buoyancy passes through each production hydrogen reactor and enters from bottom to top
In gas-liquid separating box, and enter expansion box by gas-liquid separating box；Reaction solution also under the action of the feed pump after pass through by being located at most
The production hydrogen reactor of top layer enters gas-liquid separating box, and is flowed into waste liquid collecting box from gas-liquid separating box side.
2. production hydrogen methods according to claim 1, which is characterized in that the photosynthetic bacteria that step S1 is added in is with logarithm
The photosynthetic bacteria in later stage phase is inoculated with, and with 30% bacterial load and 70% growth medium, is in intensity of illumination
3000lx, temperature are cultivated under the conditions of being 30 DEG C.
3. production hydrogen methods according to claim 1, which is characterized in that in step S1, the feed pump uses constant flow pump, leads to
The flow velocity of adjustment feed pump is crossed, the Hydraulic retention time for making system is 30-36h so that is continuously replenished photosynthetic in growth period
Bacterium discharges the photosynthetic bacteria of decline, it is ensured that photosynthetic bacteria keeps best physiological status in production hydrogen reactor.
4. production hydrogen methods according to claim 1, which is characterized in that the lighting device set by each production hydrogen reactor top surface
Include using center tube as a series of concentric circles LED light strip in the center of circle.
5. production hydrogen methods according to claim 4, which is characterized in that the LED light strip is in equidistant concentric arrays.
6. production hydrogen methods according to claim 1, which is characterized in that the bottom surface of the production hydrogen reactor is circular flat,
The inclined-plane that top surface is then gradually increased for ecto-entad.
7. production hydrogen methods according to claim 1, which is characterized in that the production hydrogen reactor is mainly by organic glass system
Into.
8. production hydrogen methods according to claim 1, which is characterized in that the feed pump is constant flow pump.
9. production hydrogen methods according to claim 1, which is characterized in that further include at least two support columns, each production hydrogen is anti-
Device is answered to be all connected to the support column.
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| CN108179100B (en) * | 2018-03-26 | 2021-06-08 | 山东百沃生物科技有限公司 | Photosynthetic bacteria screening and culturing method |
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|---|---|---|---|---|
| CN101538587A (en) * | 2009-03-23 | 2009-09-23 | 河南农业大学 | Method for continuously producing hydrogen with photosynthetic bacteria and apparatus thereof |
| CN102304468A (en) * | 2011-08-12 | 2012-01-04 | 河南农业大学 | Device for enhancing hydrogen production activity of photosynthetic bacteria |
| CN103627626A (en) * | 2013-11-05 | 2014-03-12 | 河南农业大学 | Novel optical channel photosynthetic biological hydrogen production device |
| WO2015193214A1 (en) * | 2014-06-16 | 2015-12-23 | Bayer Technology Services Gmbh | Reaction chamber for a chemical reactor, and chemical reactor constructed therefrom |
| CN205241702U (en) * | 2015-12-25 | 2016-05-18 | 河南农业大学 | H -H reaction system is produced in succession to tower photosynthetic bacteria |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101538587A (en) * | 2009-03-23 | 2009-09-23 | 河南农业大学 | Method for continuously producing hydrogen with photosynthetic bacteria and apparatus thereof |
| CN102304468A (en) * | 2011-08-12 | 2012-01-04 | 河南农业大学 | Device for enhancing hydrogen production activity of photosynthetic bacteria |
| CN103627626A (en) * | 2013-11-05 | 2014-03-12 | 河南农业大学 | Novel optical channel photosynthetic biological hydrogen production device |
| WO2015193214A1 (en) * | 2014-06-16 | 2015-12-23 | Bayer Technology Services Gmbh | Reaction chamber for a chemical reactor, and chemical reactor constructed therefrom |
| CN205241702U (en) * | 2015-12-25 | 2016-05-18 | 河南农业大学 | H -H reaction system is produced in succession to tower photosynthetic bacteria |
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