CN207294809U - A kind of neural stem cell culture flask - Google Patents
A kind of neural stem cell culture flask Download PDFInfo
- Publication number
- CN207294809U CN207294809U CN201721041906.3U CN201721041906U CN207294809U CN 207294809 U CN207294809 U CN 207294809U CN 201721041906 U CN201721041906 U CN 201721041906U CN 207294809 U CN207294809 U CN 207294809U
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- bottle
- neural stem
- stem cell
- blake bottle
- culture flask
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Abstract
The utility model discloses a kind of neural stem cell culture flask, it is related to cell biology, the neural stem cell culture flask includes blake bottle, bottle cap, side cover and permeability pipeline, bottleneck, inlet port and outlet port are respectively arranged with the top of blake bottle, blake bottle is internally provided with the permeability pipeline in infiltration cell culturing space for gases and liquids, bottle cap includes lid and head cover, and lid portion inside is provided with PTFE tissue culture films, and the inner wall of the blake bottle is provided with albumin coated layer.The utility model is optimized and improves to the material inside neural stem cell culture flask and shape, by being passed through gas or liquid into permeability pipeline, gas and liquid are entered through permeability pipeline in the cell culturing space of bottle, so as to supplement the gas and nutriment needed for cell, solve the problems such as constant and easy to pollute caused by traditional liquid feeding aerating, while improve the quantity of culture cell.
Description
Technical field
It the utility model is related to cell biology, more particularly to a kind of neural stem cell culture flask.
Background technology
Neural stem cell is a kind of stem cell being present in the tissue such as embryo or adult brain, spinal cord, is a kind of to have point
Split the mother cell of potential and self-renewal capacity, can be divided into by not reciprocity divisional mode neuron, astroglia,
The various types of cells of the nerve fibers such as oligodendroglia, also can transdifferentiation haematoblast and Skeletal Muscle Cell.Current science skill
Art can cultivate amplification neural stem cell in vitro, and neural stem cell is in vitro in incubation generally in suspended state,
It is not adherent, and have and to form the characteristic of nerve ball.The neural stem cell of in vitro culture can be used for life science, drug screening to survey
The fields such as examination, clinical application research.
And current Tissue Culture Flask is all there is a technical bottleneck, i.e., Tissue Culture Flask once cultivate the amount of cell without
Method meets the requirement of experiment, it is therefore desirable to which more than one Tissue Culture Flask carries out cell culture, causes medicine and Medical treatment device
The increase of cost in tool R＆D process, the waste of resource.Meanwhile these devices there are it is troublesome in poeration, cell density is small, condition
(such as gas componant and concentration and nutrient solution concentration) is not easy to control, cell or tissue production concentration is relatively low and is easy to loss, dirt
The shortcomings of dye.
Utility model content
In view of this, the utility model is optimized and changes to the material inside neural stem cell culture flask and shape
It is good, by being passed through gas or liquid into permeability pipeline so that gas and liquid can enter bottle through permeability pipeline
Cell culturing space in, so as to supplement the gas and nutriment needed for cell, solve traditional liquid feeding aerating institute
Bring it is constant and easy to pollute the problems such as, while improve culture cell quantity.
The utility model is solved the above problems by following technological means：
A kind of neural stem cell culture flask, it is characterised in that including blake bottle, bottle cap, side cover and permeability pipeline, its
In：Bottleneck, inlet port and outlet port are respectively arranged with the top of blake bottle, bottleneck is connected through a screw thread the bottle being adapted with bottleneck
Lid, inlet port and outlet port are connected through a screw thread the side cover being adapted with feed inlet, discharge port, are offered on the side cover respectively
Mounting hole, blake bottle is internally provided with the permeability pipeline in infiltration cell culturing space for gases and liquids, described penetrating
Property pipeline include feed end and discharge end, feed end is connected with the mounting hole of feed inlet, and the mounting hole of discharge end and discharge port connects
It is logical；The bottle cap includes lid and head cover, and the side of head cover connects lid by shaft, and the opposite side of head cover is connected by latching
Lid, lid portion inside are provided with PTFE tissue culture films, and the PTFE tissue cultures film is less than the netted knot of microporous bodies of 0.15um for aperture
Structure；The inner wall of the blake bottle is provided with albumin coated layer, and the albumin coated layer is with physical absorption or covalent cross-linking
Mode is attached on the inner wall of blake bottle, and the albumin coated layer is human serum albumin coated layer.
Further, the bottom of the blake bottle is provided with erecting bed, and the cell of annular is fixed with the inner cavity of blake bottle
Adherent dielectric layer, cell attachment dielectric layer are separated with the inner wall of blake bottle, and cell attachment dielectric layer is non-woven fabrics plicated layer, nonwoven
Cloth plicated layer from erecting bed be fixed on the inner cavity of bottle to.
Further, the permeability pipeline is made of the permselective membrane with loose structure, and selectivity is logical
The aperture in permeable membrane hole is less than or equal to 2.5 microns.
Further, the thickness of non-woven fabrics used in the non-woven fabrics plicated layer is 1-1.5mm, and is by polyester fiber
What braiding formed, a diameter of 15-18 μm of the polyester fiber.
Further, the two sides of the inner wall of the bottle and non-woven fabrics plicated layer is surface-treated by vacuum plasma.
A kind of neural stem cell culture flask of the utility model has the advantages that：
The utility model discloses a kind of neural stem cell culture flask, it is related to cell biology, the neural stem cell
Blake bottle includes blake bottle, bottle cap, side cover and permeability pipeline, is respectively arranged with bottleneck, feed inlet at the top of blake bottle and goes out
Material mouth, blake bottle are internally provided with the permeability pipeline in infiltration cell culturing space for gases and liquids, and bottle cap includes lid
Body and head cover, lid portion inside are provided with PTFE tissue culture films, and the inner wall of the blake bottle is provided with albumin coated layer.This practicality is new
Type is optimized and improves to the material inside neural stem cell culture flask and shape, by being passed through gas into permeability pipeline
Body or liquid so that gas and liquid can pass through permeability pipeline and enter in the cell culturing space of bottle, so as to mend
The gas and nutriment needed for cell are filled, solves the problems such as constant and easy to pollute caused by traditional liquid feeding aerating, together
When improve culture cell quantity.
Compared with the flat cell culture container of tradition, the inner wall surface of blake bottle, which adds one layer of albumin coated layer, to be reduced
Absorption of the culture vessel inner wall to neural stem cell can be stood, so that the residual of culture vessel can found by greatly reducing neural stem cell
Stay rate, be conducive to prevent neural stem cell it is adherent, maintain neural stem cell suspended state, more easily form nerve ball.Together
When, due to adding cell attachment dielectric layer in the inner cavity of the bottle of Tissue Culture Flask, adding can in Tissue Culture Flask
The surface area of cell attachment culture is carried out, therefore, in cell cultivation process, cell density adherent in Tissue Culture Flask increases
Add, so as to improve the amount that Tissue Culture Flask once cultivates cell.
Brief description of the drawings
, below will be to attached needed in embodiment in order to illustrate more clearly of the technical solution of the utility model
Figure is briefly described, it should be apparent that, drawings in the following description are only some embodiments of the utility model, for
For those of ordinary skill in the art, without creative efforts, other can also be obtained according to these attached drawings
Attached drawing.
Fig. 1 is a kind of structure diagram of neural stem cell culture flask of the utility model；
Fig. 2 is a kind of Bottle cap structure schematic diagram of neural stem cell culture flask of the utility model.
The title of each number designation is respectively in figure：1- blake bottles, 2- bottlenecks, 3- bottle caps, 4- feed inlets, 5- discharge ports,
6- side covers, 7- permeabilities pipeline, 8- erecting beds, 9- cell attachments dielectric layer, 10- albumin coated layer, 31- lids, 32- tops
Lid, 33- shafts, 34- lock, 35-PTFE tissue culture films.
Embodiment
In the description of the utility model, it is to be understood that term " " center ", " on ", " under ", "front", "rear",
The orientation or position relationship of the instruction such as "left", "right", " vertical ", " level ", " top ", " bottom ", " interior ", " outer " is based on attached drawing institutes
The orientation or position relationship shown, is for only for ease of description the utility model and simplifies description, rather than indicates or imply and is signified
Device or element must have specific orientation, with specific azimuth configuration and operation, therefore it is not intended that to this practicality
New limitation.Term " first ", " second " be only used for description purpose, and it is not intended that instruction or imply relative importance or
Person implies the quantity for indicating indicated technical characteristic.Thus, " first " is defined, the feature of " second " can be expressed or hidden
Include one or more this feature containing ground.In the description of the utility model, unless otherwise indicated, " multiple " are meant that
Two or more.
The utility model is described in detail below with reference to attached drawing.
As shown in Figure 1 and Figure 2, a kind of neural stem cell culture flask, including blake bottle 1, bottle cap 3, side cover 6 and permeability pipe
Road 7, wherein：The top of blake bottle 1 is respectively arranged with bottleneck 2, feed inlet 4 and discharge port 5, and bottleneck 2 is connected through a screw thread and bottle
The bottle caps 3 that mouth 2 is adapted, feed inlet 4 and discharge port 5 are connected through a screw thread the side being adapted with feed inlet 4, discharge port 5 respectively
Lid 6, mounting hole is offered on the side cover 6, and blake bottle 1 is internally provided with infiltration cell culturing space for gases and liquids
Permeability pipeline 7, the permeability pipeline 7 includes feed end and discharge end, and feed end is connected with the mounting hole of feed inlet 4,
Discharge end is connected with the mounting hole of discharge port 5.
Specifically, in cell cultivation process, gas and/or battalion are passed through by continuing into permeability pipeline 7 or being interrupted
Material etc. is supported, so as to the gas and the concentration of nutriment being replenished in time in bottle, to maintain cell growth required
Condition of culture, solves gas in prior art blake bottle 1 and nutrient concentrations regulation and control are constant, complicated easy to pollute etc. to ask
Topic.
As shown in Fig. 2, bottle cap 3 includes lid 31 and head cover, the side of head cover 32 connects lid 31, head cover by shaft 33
32 opposite side is internally provided with PTFE tissue cultures film 35, the PTFE tissue cultures film 35 by the connection lid 31 of lock 34, lid 31
It is less than the microporous bodies net structure of 0.15um for aperture.
Specifically, PTFE tissue cultures film is under conditions of air exchange is ensured, while can be to avoid bacterium and cell entry
In bottle, achieve the purpose that to prevent pollution.
The inner wall of the blake bottle 1 is provided with albumin coated layer 10, the albumin coated layer 10 with physical absorption or
The mode of covalent cross-linking is attached on the inner wall of blake bottle 1, and the albumin coated layer 10 is human serum albumin coated layer 10.
The present embodiment can be manufactured or realized by the following means：(1) mould and macromolecule polymer material polyphenyl are used
Ethene makes 3 one-tenth shapes as shown in Figure 1, 2 of body and bottle cap；(2) the 3% human serum albumin solution prepared with DPBS is poured into
In bottle, human serum albumin is set to be substantially adhered on inner wall, to form coated layer, then use ultra-pure water overnight in 40 DEG C of incubator
Wash, dry.
Specifically, the bottom of the blake bottle 1 is provided with erecting bed 8, the cell of annular is fixed with the inner cavity of blake bottle 1
Adherent dielectric layer 9, cell attachment dielectric layer 9 are separated with the inner wall of blake bottle 1, and cell attachment dielectric layer 9 is non-woven fabrics plicated layer,
Non-woven fabrics plicated layer from erecting bed 8 be fixed on the inner cavity of bottle to.
Specifically, the permeability pipeline 7 is the selective permeation made of the permselective membrane with loose structure
The aperture of fenestra is less than or equal to 2.5 microns.
Specifically, the thickness of non-woven fabrics used in the non-woven fabrics plicated layer is 1-1.5mm, and compiled by polyester fiber
Knit, a diameter of 15-18 μm of the polyester fiber.
Specifically, the inner wall of the bottle and the two sides of non-woven fabrics plicated layer are surface-treated by vacuum plasma.
Specifically, it is using the reason for non-woven fabrics plicated layer：The permeability and permeability of non-woven fabrics are all fine,
It can ensure the inner and outer wall in the non-woven fabrics plicated layer, and supporting needed for cell growth adherent on the inner wall of bottle
The supply of material and/or oxygen can obtain good satisfaction, and survival rate of the cell in incubation has obtained significant raising.
A kind of neural stem cell culture flask 1 of the utility model has the advantages that：
The utility model discloses a kind of neural stem cell culture flask 1, it is related to cell biology, the neural stem cell
Blake bottle 1 includes blake bottle 1, bottle cap 3, side cover 6 and permeability pipeline 7, and the top of blake bottle 1 is respectively arranged with bottleneck 2, charging
Mouth 4 and discharge port 5, blake bottle 1 are internally provided with the permeability pipeline 7 in infiltration cell culturing space for gases and liquids,
Bottle cap 3 includes lid 31 and head cover 32, and lid 31 is internally provided with PTFE tissue culture films, and the inner wall of the blake bottle 1 is provided with white
Albumen coated layer 10.The utility model is optimized and improves to the material inside neural stem cell culture flask 1 and shape, leads to
Cross into permeability pipeline 7 and be passed through gas or liquid so that gas and liquid can pass through permeability pipeline 7 and enter the thin of bottle
In born of the same parents' culture space, so as to supplement the gas and nutriment needed for cell, solve traditional liquid feeding aerating and brought
It is constant and easy to pollute the problems such as, while improve culture cell quantity.
Compared with the flat cell culture container of tradition, the inner wall surface of blake bottle 1 is added one layer of albumin coated layer 10 and is subtracted
The absorption that can found culture vessel inner wall to neural stem cell is lacked, so that culture vessel can found by greatly reducing neural stem cell
Residual rate, be conducive to prevent neural stem cell it is adherent, maintain neural stem cell suspended state, more easily formed nerve
Ball.Simultaneously as adding cell attachment dielectric layer 9 in the inner cavity of the bottle of Tissue Culture Flask 1, Tissue Culture Flask is added
The surface area of cell attachment culture can be carried out in 1, therefore, in cell cultivation process, cell is adherent in Tissue Culture Flask 1
Density increase, so as to improve the amount that Tissue Culture Flask 1 once cultivates cell.
Finally it should be noted that：Above example is only to illustrate the technical solution of the utility model, rather than its limitations；
Although the utility model is described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that：
It can still modify the technical solution described in foregoing embodiments, or which part technical characteristic is carried out etc.
With replacement；And these modifications or replacement, the essence of appropriate technical solution is departed from various embodiments of the utility model technology
The spirit and scope of scheme.
Claims (5)
- A kind of 1. neural stem cell culture flask, it is characterised in that including blake bottle, bottle cap, side cover and permeability pipeline, wherein： Bottleneck, inlet port and outlet port are respectively arranged with the top of blake bottle, bottleneck is connected through a screw thread the bottle cap being adapted with bottleneck, Inlet port and outlet port are connected through a screw thread the side cover being adapted with feed inlet, discharge port respectively, and peace is offered on the side cover Hole is filled, blake bottle is internally provided with the permeability pipeline in infiltration cell culturing space for gases and liquids, the permeability Pipeline includes feed end and discharge end, and feed end is connected with the mounting hole of feed inlet, and discharge end is connected with the mounting hole of discharge port； The bottle cap includes lid and head cover, and the side of head cover connects lid by shaft, and the opposite side of head cover is by latching connection cover Body, lid portion inside are provided with PTFE tissue culture films, and the PTFE tissue cultures film is less than the microporous bodies net structure of 0.15um for aperture； The inner wall of the blake bottle is provided with albumin coated layer, and the albumin coated layer is in a manner of physical absorption or covalent cross-linking It is attached on the inner wall of blake bottle, the albumin coated layer is human serum albumin coated layer.
- 2. neural stem cell culture flask as claimed in claim 1, it is characterised in that the bottom of the blake bottle is provided with installation Platform, the cell attachment dielectric layer of annular is fixed with the inner cavity of blake bottle, and cell attachment dielectric layer is separated with the inner wall of blake bottle, Cell attachment dielectric layer is non-woven fabrics plicated layer, non-woven fabrics plicated layer from erecting bed be fixed on the inner cavity of bottle to.
- 3. neural stem cell culture flask as claimed in claim 2, it is characterised in that the permeability pipeline is by with more Made of the permselective membrane of pore structure, the aperture of selective permeation fenestra is less than or equal to 2 .5 microns.
- 4. neural stem cell culture flask as claimed in claim 3, it is characterised in that used in the non-woven fabrics plicated layer The thickness of non-woven fabrics is 1-1.5mm, and is formed by polyester fiber braiding, a diameter of 15-18 μm of the polyester fiber.
- 5. neural stem cell culture flask as claimed in claim 4, it is characterised in that：The inner wall and non-woven fabrics fold of the bottle The two sides of layer is surface-treated by vacuum plasma.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721041906.3U CN207294809U (en) | 2017-08-20 | 2017-08-20 | A kind of neural stem cell culture flask |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721041906.3U CN207294809U (en) | 2017-08-20 | 2017-08-20 | A kind of neural stem cell culture flask |
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| Publication Number | Publication Date |
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| CN207294809U true CN207294809U (en) | 2018-05-01 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721041906.3U Expired - Fee Related CN207294809U (en) | 2017-08-20 | 2017-08-20 | A kind of neural stem cell culture flask |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108085254A (en) * | 2018-02-09 | 2018-05-29 | 中国人民解放军第四军医大学 | Tissue engineering product culture apparatus |
| CN110283725A (en) * | 2019-07-02 | 2019-09-27 | 英诺维尔智能科技（苏州）有限公司 | A kind of novel multi-layer attached cell culture vessel structure |
| CN116179342A (en) * | 2022-11-15 | 2023-05-30 | 东营凤起生物科技发展有限公司 | Neural stem cell expansion device and method thereof |
-
2017
- 2017-08-20 CN CN201721041906.3U patent/CN207294809U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108085254A (en) * | 2018-02-09 | 2018-05-29 | 中国人民解放军第四军医大学 | Tissue engineering product culture apparatus |
| CN110283725A (en) * | 2019-07-02 | 2019-09-27 | 英诺维尔智能科技（苏州）有限公司 | A kind of novel multi-layer attached cell culture vessel structure |
| CN116179342A (en) * | 2022-11-15 | 2023-05-30 | 东营凤起生物科技发展有限公司 | Neural stem cell expansion device and method thereof |
| CN116179342B (en) * | 2022-11-15 | 2023-12-19 | 东营凤起生物科技发展有限公司 | Neural stem cell expansion device and method thereof |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180501 Termination date: 20210820 |
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| CF01 | Termination of patent right due to non-payment of annual fee |