CN108085254A - Tissue engineering product culture apparatus - Google Patents
Tissue engineering product culture apparatus Download PDFInfo
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- CN108085254A CN108085254A CN201810132109.9A CN201810132109A CN108085254A CN 108085254 A CN108085254 A CN 108085254A CN 201810132109 A CN201810132109 A CN 201810132109A CN 108085254 A CN108085254 A CN 108085254A
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- 210000001519 tissue Anatomy 0.000 claims description 48
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- 238000009423 ventilation Methods 0.000 claims description 8
- 230000002572 peristaltic effect Effects 0.000 claims description 6
- 238000012549 training Methods 0.000 claims description 4
- 238000005086 pumping Methods 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 2
- 239000000463 material Substances 0.000 abstract description 20
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- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/02—Filters
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
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Abstract
The invention discloses a kind of tissue engineering product culture apparatus, including:Culture solution holding vessel, culture solution circulation line and biochemical culture storehouse;For culture solution holding vessel for storing culture solution, biochemical culture storehouse is used for cultured tissue engineering product;Culture solution holding vessel is connected by culture solution circulation line with biochemical culture storehouse with to biochemical culture storehouse circulation conveying culture solution;Wherein, biochemical culture storehouse and culture solution circulation line are separably attached.So as to after the completion of tissue engineering product culture, directly biochemical culture storehouse is removed and is transported to medical institutions, without by cultured tissue engineering product by taking out and repacking in reactor.It solves the problems, such as directly to be easy to cause pollution in culture and transport by the organization material of bioreactor for constructing in the prior art.
Description
Technical field
The present invention relates to organizational projects and regenerative medicine field, are filled in particular to a kind of tissue engineering product culture
It puts.
Background technology
Various wounds, infection and the treatment of organ defect caused by disease are still one of difficult medical problem, regenerative medicine and group
The structure " safe and effective histoorgan graft substitute " that develops into for knitting engineering technology brings new hope.
Tissue-engineering graft constructed mainly by material and cellularity, wherein material include artificial bone, blood vessel, nerve, skin,
Internal organ etc. can be widely used for repairing tissue defect;Cell using it is wide have mescenchymal stem cell, candidate stem cell and
Artificial multipotential stem cell etc..Material provides stent for cell adherence and migration, and cell assigns material biological activity, and the two is mutually auxiliary
It coordinates, it is indispensable.
External structure and transport are the most essential steps that clinic is moved towards from laboratory of tissue engineering product.External structure is
Autologous patient cell and material is compound in vitro in advance and maintain a period of time, it, can so that cell adapted material microenvironment
It is survived with stablizing, multiplication.
In order to improve cell viability, at present mostly using bioreactor for constructing material, but bioreactor construction is complicated, behaviour
It is low to make cumbersome and security.In addition, it is transported by the graft after bioreactor culture from laboratory to the when need of operating room
To pass through multiple steps, in particular it is required that taking out graft-packaging (keep sample detection)-transport-use way out of bioreactor
In, step is more, easily pollutes.
The content of the invention
It is a primary object of the present invention to provide a kind of tissue engineering product culture apparatus, at least to solve in the prior art
The problem of pollution is directly be easy to cause in culture and transport by the organization material of bioreactor culture.
To achieve these goals, the present invention provides a kind of tissue engineering product culture apparatus, including:Culture solution stores
Tank, culture solution circulation line and biochemical culture storehouse;For storing culture solution, biochemical culture storehouse is used to cultivate group culture solution holding vessel
Knit engineering product;Culture solution holding vessel connects to cycle to biochemical culture storehouse with biochemical culture storehouse by culture solution circulation line defeated
Send culture solution;Wherein, biochemical culture storehouse and culture solution circulation line are separably attached.
Further, culture solution circulation line includes:Liquid pushing tube, first port and the culture solution holding vessel of liquid pushing tube connect
It is logical, the second port of liquid pushing tube connected from the bottom in biochemical culture storehouse with biochemical culture storehouse with by the bottom in biochemical culture storehouse to
Culture solution is conveyed in biochemical culture storehouse;Peristaltic pump is arranged on liquid pushing tube, to apply pumping pressure into liquid pushing tube;Wherein, send
The first valve is provided in liquid pipe, the first valve is used to control the conducting and disconnection of liquid pushing tube.
Further, culture solution circulation line further includes:Liquid back pipe, the first port of liquid back pipe by biochemical culture storehouse top
Portion is connected with biochemical culture storehouse, and the second port of liquid back pipe connects the culture solution so that in biochemical culture storehouse with culture solution holding vessel
It is back in culture solution holding vessel;Wherein, liquid back pipe is provided with the second valve, the second valve be used to control liquid back pipe conducting and
It disconnects.
Further, the first valve and the second valve are double-deck valve.
Further, the first valve is positioned close to one end of the second port of liquid pushing tube;Second valve is positioned close to
One end of the first port of liquid back pipe.
Further, tissue engineering product culture apparatus further includes:Nutrient canal is worn along the short transverse in biochemical culture storehouse
In biochemical culture storehouse, nutrient canal has the feed liquor port being located at outside biochemical culture storehouse and liquid returning end mouth;Wherein, liquid pushing tube
Second port connects to convey culture solution into biochemical culture storehouse with the feed liquor port of nutrient canal, and the liquid returning end mouth of nutrient canal is with returning
The first port of liquid pipe is connected so that the culture solution in biochemical culture storehouse is back in culture solution holding vessel.
Further, liquid drain hole is offered on nutrient canal, liquid drain hole is multiple;Wherein, nutrient canal passes through liquid drain hole and life
The culture solution changed in culture storehouse carries out cycling exchange.
Further, ventilation pipe, first port and the culture solution holding vessel of pipe of taking a breath are provided on culture solution holding vessel
Inside connects, and sterilised membrane filter is provided in the second port for pipe of taking a breath, and sterilised membrane filter is used to that extraneous air to be made after filtering to lead to
Ventilation pipe is crossed to enter in culture solution holding vessel.
Further, feed inlet is provided on biochemical culture storehouse, feed inlet is double-deck Self-enclosing.
Further, connecting component is provided on biochemical culture storehouse, connecting component is fishing without hook or buckle, and connecting component is used for
Biochemical culture storehouse is fixedly connected on consersion unit.
Using the tissue engineering product culture apparatus of technical solution of the present invention, including:Culture solution holding vessel, culture solution Xun Huan
Pipeline and biochemical culture storehouse;For culture solution holding vessel for storing culture solution, biochemical culture storehouse is used for cultured tissue engineering product;Training
Nutrient solution holding vessel is connected by culture solution circulation line with biochemical culture storehouse with to biochemical culture storehouse circulation conveying culture solution;Its
In, biochemical culture storehouse and culture solution circulation line are separably attached.Thus after the completion of tissue engineering product culture, directly will
Biochemical culture storehouse removes and is transported to medical institutions, without by cultured tissue engineering product by reactor take out and again
Packaging.It solves and dirt is directly be easy to cause in culture and transport by the organization material of bioreactor for constructing in the prior art
The problem of dye.
Description of the drawings
Attached drawing described herein is used for providing a further understanding of the present invention, forms the part of the application, this hair
Bright schematic description and description does not constitute improper limitations of the present invention for explaining the present invention.In the accompanying drawings:
Fig. 1 is optional a kind of structure diagram of tissue engineering product culture apparatus according to embodiments of the present invention.
Wherein, above-mentioned attached drawing is marked including the following drawings:
10th, culture solution holding vessel;11st, ventilation pipe;12nd, sterilised membrane filter;20th, culture solution circulation line;21st, liquid pushing tube;22、
Liquid back pipe;23rd, the first valve;24th, the second valve;25th, peristaltic pump;30th, biochemical culture storehouse;31st, feed inlet;32nd, connecting component;
40th, nutrient canal;41st, feed liquor port;42nd, liquid returning end mouth;43rd, liquid drain hole.
Specific embodiment
In order to which those skilled in the art is made to more fully understand the present invention program, below in conjunction in the embodiment of the present invention
The technical solution in the embodiment of the present invention is clearly and completely described in attached drawing, it is clear that described embodiment is only
The embodiment of a part of the invention, instead of all the embodiments.Based on the embodiments of the present invention, ordinary skill people
Member's all other embodiments obtained without making creative work should all belong to the model that the present invention protects
It encloses.
Tissue engineering product culture apparatus according to embodiments of the present invention, as shown in Figure 1, including culture solution holding vessel 10,
Culture solution circulation line 20 and biochemical culture storehouse 30;For storing culture solution, biochemical culture storehouse 30 is used for culture solution holding vessel 10
Cultured tissue engineering product;Culture solution holding vessel 10 is connected by culture solution circulation line 20 with biochemical culture storehouse 30 with to biochemistry
Cultivate 30 circulation conveying culture solution of storehouse;Wherein, biochemical culture storehouse 30 and culture solution circulation line 20 are separably attached.
Using the tissue engineering product culture apparatus of the embodiment of the present invention, cycled including culture solution holding vessel 10, culture solution
Pipeline 20 and biochemical culture storehouse 30;For culture solution holding vessel 10 for storing culture solution, biochemical culture storehouse 30 is used for cultured tissue work
Journey product;Culture solution holding vessel 10 connects to follow to biochemical culture storehouse 30 with biochemical culture storehouse 30 by culture solution circulation line 20
Ring conveys culture solution;Wherein, biochemical culture storehouse 30 and culture solution circulation line 20 are separably attached.So as to be produced in organizational project
After the completion of product culture, directly biochemical culture storehouse 30 is removed and is transported to medical institutions, without cultured organizational project is produced
Product are by taking out and repacking in reactor.It solves and is directly existed in the prior art by the organization material of bioreactor for constructing
The problem of pollution being be easy to cause when culture and transport.
When it is implemented, it is necessary to constantly supplement carbon dioxide in culture solution in biological tissue's incubation.
Therefore, ventilation pipe 11 is provided on culture solution holding vessel 10, first port and the culture solution holding vessel 10 of pipe 11 of taking a breath it is interior
Portion connects, and is provided with sterilised membrane filter 12 in the second port for pipe 11 of taking a breath, sterilised membrane filter 12 can be by the bacterium in extraneous air
With in the culture solution that is passed through after contaminant filter in culture solution holding vessel 10 by pipe 11 of taking a breath, so as to be constantly infused for culture solution
Carbon dioxide.Culture solution holding vessel 10 is placed in entire reaction process in cell incubator, is carried out by sterilised membrane filter 12
Gas exchanges meet the needs of cell survival, growth are to carbon dioxide.
Further, feed inlet 31 is provided on biochemical culture storehouse 30, feed inlet 31 is double-deck Self-enclosing.Pass through feed inlet
31 can be put into tissue engineering product to be cultivated in biochemical culture storehouse 30.After being put into tissue engineering product, by bilayer certainly
Biochemical culture storehouse 30 can be sealed to seal tissue engineering product up for safekeeping and ensure it in vitro in building process in sterile by sealing
Environment.The tissue engineering product in biochemical culture storehouse 30 is put into using culture solution as medium, is subjected to rotation, concussion, pulsed power
Learning stimulates, and strengthens cell function and takes exercise, improves its survival rate so as to increase product efficiency.
The tissue engineering product of structure includes, using 3D printing technique, being designed according to conditions of patients in biochemical culture storehouse 30
Individuation material, such as the bone tissue of defect, blood vessel, tracheae, nerve, muscle, skin, internal organ, to realize precisely medical treatment.It can be with
The appropriate biochemical culture storehouse 30 of size is selected according to the size of the tissue engineering product of structure, to save culture solution and cost.
Culture solution circulation line 20 includes:Liquid pushing tube 21 and liquid back pipe 22, first port and the culture solution of liquid pushing tube 21 store up
Tank 10 is deposited to connect.Specifically, the first port of liquid pushing tube 21 enters culture solution holding vessel 10 by the top of culture solution holding vessel 10
Inside simultaneously extends to the bottom of culture solution holding vessel 10, so that the first port of liquid pushing tube 21 can be located at culture solution
Liquid level is once.The second port of liquid pushing tube 21 is connected by the bottom in biochemical culture storehouse 30 with biochemical culture storehouse 30;In liquid pushing tube 21
On be provided with peristaltic pump 25, peristaltic pump 25 applies pumping pressure into liquid pushing tube 21 so as to by the culture solution of culture solution holding vessel 10
It is pumped by liquid pushing tube 21 to biochemical culture storehouse 30.The flow direction and flow velocity of culture solution can be adjusted by peristaltic pump 25.
The first port of liquid back pipe 22 is connected by the top in biochemical culture storehouse 30 with biochemical culture storehouse 30, and the of liquid back pipe 22
Two-port netwerk is connected with culture solution holding vessel 10.Specifically, the second port of liquid back pipe 22 by culture solution holding vessel 10 top into
Enter inside culture solution holding vessel 10, the second port of liquid back pipe 22 is located at the upper space of culture solution holding vessel 10.It pumps to life
Change culture solution in culture storehouse 30 with the rising of liquid level and finally overflowed from liquid back pipe 22 and be back to culture solution holding vessel 10
It realizes the circulation conveying of culture solution, the culture solution for incorporating carbon dioxide is constantly delivered in biochemical culture storehouse 30.
The first valve 23 is provided on liquid pushing tube 21, the first valve 23 is used to control the conducting and disconnection of liquid pushing tube 21;It returns
Liquid pipe 22 is provided with the second valve 24, and the second valve 24 is used to control the conducting and disconnection of liquid back pipe 22;First valve 23 is set
In one end of the second port close to liquid pushing tube 21;Second valve 24 is positioned close to one end of the first port of liquid back pipe 22.
So as to be turned off the first valve 23 and the second valve 24 after tissue cultures are completed, make to form confined air in biochemical culture storehouse 30
Between, then liquid pushing tube 21 and liquid back pipe 22 are cut close to the one side of culture solution holding vessel 10 from the first valve 23 and the second valve 24
It is disconnected, biochemical culture storehouse 30 is made to be separated with culture solution circulation line 20, you can biochemical culture storehouse 30 is directly transported to operating room,
During this, the tissue engineering product of culture is in always in space closed in biochemical culture storehouse 30, will not cause bacterium sense
Dye and pollution ensure normal survival, breeding and the vigor of biological tissue, the final success rate for ensureing operation.
Further, in order to strengthen the sealing effect to biochemical culture storehouse 30, optionally, the first valve 23 and the second valve
24 be double-deck valve.
In order to ensure circulating effect of the culture solution in biochemical culture storehouse 30, increase carbon dioxide in biochemical culture storehouse 30
Internal circulating load, further, tissue engineering product tissue engineering product culture apparatus further includes nutrient canal 40, and nutrient canal 40 is along life
The short transverse for changing culture storehouse 30 is threaded through in biochemical culture storehouse 30.Specifically, nutrient canal 40 is removably mounted on biochemical training
It supports in storehouse 30.
Nutrient canal 40 has the feed liquor port 41 being located at outside biochemical culture storehouse 30 and liquid returning end mouth 42;Feed liquor port 41
In the bottom in biochemical culture storehouse 30, liquid returning end mouth 42 is located at the top in biochemical culture storehouse 30;The second port of liquid pushing tube 21 and battalion
The feed liquor port 41 for supporting pipe 40 connects, and liquid drain hole 43 is offered on nutrient canal 40, and liquid drain hole 43 is multiple, and culture solution passes through feed liquor
Cycling is carried out after the entrance of port 41 nutrient canal 40 with the culture solution in biochemical culture storehouse 30 by liquid drain hole 43 to exchange.Nutrient canal 40
Liquid returning end mouth 42 connected with the first port of liquid back pipe 22 so that culture solution of the biochemical culture storehouse 30 after cycling is back to
In culture solution holding vessel 10.By setting nutrient canal 40 that can cultivate big section tissue engineering product, nutrient canal 40 is trained through biochemical
30 center of storehouse is supported, culture solution is supplied from inside, improves Cell viability comprehensively
The tissue engineering product tissue engineering product culture apparatus of the present embodiment has the function of part biological reactor, also
It can be cultivated with reference to other consersion units, specifically, connecting component 32 is provided on the outer wall in biochemical culture storehouse 30, even
Relay part 32 is fishing without hook or buckle, and optionally, thread gluing or buckle are provided with two pairs, can be by biochemical culture by thread gluing or buckle
Storehouse 30 is fixedly connected in bioreactor or other 3D culture devices, realizes the mechanical stimulations such as rotation, concussion, pulse, simulation
Internal mechanical environment realizes 3D cultures, greatly facilitates survival of the target cell in material and lapses to, so as to enhance organizational project
The effective performance of product, and easy to operate, safety.
Optionally, culture solution holding vessel 10, culture solution circulation line 20 and biochemical culture storehouse 30 can be used such as polyurethane, gather
The materials such as ethylene, polypropylene, silicon rubber, polyvinyl chloride, sulfone, polyamide are made.Liquid pushing tube 21 in culture solution circulation line 20
It is medical disposable material with liquid back pipe 22 and biochemical culture storehouse 30, it is directly discarded after use, cross-infection can be reduced.Training
Other accessories and culture solution holding vessel 10 in nutrient solution circulation line 20 can be reused after high pressure or irradiation sterilization.
The external structure transport integrated apparatus of the present invention, can complete direct closing valve in structure, cut off nutrient canal
Transport extremely operation unit afterwards.Special Self-enclosing and valve is disposed as double-deck series connection, safer and more effective.Reduce intermediate link
Integrated apparatus, can necessarily promote regeneration and the development of translational medicine, establish solid foundation to clinical practice for scientific research.
Tissue engineering product granted at present is more, is related to multiple subject necks such as orthopaedics, skin, breathing, angiocarpy, digestion
Domain, but most materials are free of cell, and bioactivity is low.In addition, the defect location of few patients, shape differ, common material is difficult
Wound notch is agreed with perfection, generally requires the trim materials on operating table, increases operating time and difficulty.The tissue of the present invention
Engineering product culture apparatus is directly applied after not only being pre-loaded with finished-product material culture, can also temporarily be loaded according to conditions of patients
Customized personalized material is designed, realizes precisely medical treatment.
Domestic granted stem cell and regenerative medicine GMP laboratories are less at present, and have one between laboratory and operating room
Set a distance.How the completion of tissue engineering product external structure is transported to operating room, is always that translational medicine field is urgently to be resolved hurrily
Problem.
The tissue engineering product external structure transport integrated apparatus of the present invention is easy to operate, convenient, can be in very great Cheng
The validity and security of tissue engineering product are improved on degree, solves product external structure-transport traditional technology complex steps,
The problem of easy to pollute and cell survival rate is low.
It the above is only the preferred embodiment of the present invention, it is noted that come for those skilled in the art
It says, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications also should be regarded as
Protection scope of the present invention.
Claims (10)
1. a kind of tissue engineering product culture apparatus, which is characterized in that including:
Culture solution holding vessel (10), culture solution circulation line (20) and biochemical culture storehouse (30);
The culture solution holding vessel (10) for storing culture solution, for cultured tissue engineering produce by the biochemical culture storehouse (30)
Product;The culture solution holding vessel (10) connected by the culture solution circulation line (20) with the biochemical culture storehouse (30) with to
Biochemical culture storehouse (30) the circulation conveying culture solution;
Wherein, the biochemical culture storehouse (30) and the culture solution circulation line (20) are separably attached.
2. tissue engineering product culture apparatus according to claim 1, which is characterized in that the culture solution circulation line
(20) include:
Liquid pushing tube (21), the first port of the liquid pushing tube (21) are connected with the culture solution holding vessel (10), the liquid pushing tube
(21) second port is connected with the biochemical culture storehouse (30) with by the life by the bottom of the biochemical culture storehouse (30)
The bottom for changing culture storehouse (30) conveys culture solution into the biochemical culture storehouse (30);
Peristaltic pump (25) is arranged on the liquid pushing tube (21), to apply pumping pressure into the liquid pushing tube (21);
Wherein, the first valve (23) is provided on the liquid pushing tube (21), first valve (23) is used to control the liquor charging
Manage the conducting and disconnection of (21).
3. tissue engineering product culture apparatus according to claim 2, which is characterized in that the culture solution circulation line
(20) further include:
Liquid back pipe (22), the first port of the liquid back pipe (22) are trained by the top of the biochemical culture storehouse (30) with the biochemistry
Storehouse (30) connection is supported, the second port of the liquid back pipe (22) is connected with the culture solution holding vessel (10) so that the biochemical training
The culture solution in storehouse (30) is supported to be back in the culture solution holding vessel (10);
Wherein, the liquid back pipe (22) is provided with the second valve (24), and second valve (24) is used to control the liquid back pipe
(22) conducting and disconnection.
4. tissue engineering product culture apparatus according to claim 3, which is characterized in that first valve (23) and institute
It is double-deck valve to state the second valve (24).
5. tissue engineering product culture apparatus according to claim 3, which is characterized in that first valve (23) is set
In one end of the second port close to the liquid pushing tube (21);Second valve (24) is positioned close to the liquid back pipe (22)
First port one end.
6. tissue engineering product culture apparatus according to claim 3, which is characterized in that the tissue engineering product culture
Device further includes:
Nutrient canal (40) is threaded through along the short transverse of the biochemical culture storehouse (30) in the biochemical culture storehouse (30), described
Nutrient canal (40), which has, is located at the external feed liquor port (41) of the biochemical culture storehouse (30) and liquid returning end mouth (42);
Wherein, the second port of the liquid pushing tube (21) is connected with the feed liquor port (41) of the nutrient canal (40) with to institute
State conveying culture solution in biochemical culture storehouse (30), the liquid returning end mouth (42) and the liquid back pipe (22) of the nutrient canal (40)
First port connect so that the culture solution in the biochemical culture storehouse (30) is back in the culture solution holding vessel (10).
7. tissue engineering product culture apparatus according to claim 6, which is characterized in that opened up on the nutrient canal (40)
There is liquid drain hole (43), the liquid drain hole (43) is multiple;
Wherein, the nutrient canal (40) is followed by the liquid drain hole (43) and the culture solution in the biochemical culture storehouse (30)
Ring exchanges.
8. tissue engineering product culture apparatus according to claim 1, which is characterized in that the culture solution holding vessel (10)
On be provided with ventilation pipe (11), it is described ventilation pipe (11) first port connected with the inside of the culture solution holding vessel (10),
Sterilised membrane filter (12) is provided in the second port of the ventilation pipe (11), the sterilised membrane filter (12) is used to pass through extraneous air
(11) are managed after filtering by the ventilation to enter in the culture solution holding vessel (10).
9. tissue engineering product culture apparatus according to claim 1, which is characterized in that on the biochemical culture storehouse (30)
Feed inlet (31) is provided with, the feed inlet (31) is double-deck Self-enclosing.
10. tissue engineering product culture apparatus according to claim 1, which is characterized in that the biochemical culture storehouse (30)
On be provided with connecting component (32), the connecting component (32) is fishing without hook or buckle, the connecting component (32) be used for will described in
Biochemical culture storehouse (30) is fixedly connected on consersion unit.
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